高危亚型HPV33和HPV52感染与宫颈病变的关系

目的 探讨人乳头瘤病毒(HPV)高危亚型HPV33和HPV52感染与宫颈病变的关系。方法 选取316例于我院进行病理组织活检联合HPVDNA检测的病例,对其进行HPVDNA提取、PCR扩增、导流杂交,其中对194例HPV33和HPV52亚型阳性患者资料进行分析。结果 194例HPV33和HPV52阳性患者中,宫颈良性病变225例,宫颈上皮内瘤变(CIN)为114例和宫颈恶性肿瘤为55例,利用统计学χ~2检验发现在HPV33和HPV52阳性患者中,CIN和宫颈恶性肿瘤的阳性率明显高于宫颈良性病变的阳性率(p<0.05)。结论 HPV33和HPV52阳性患者患宫颈病变的几率增大,高危型HPV33和HPV52是引起宫颈恶性病变的致病基因型。     

三氯醋酸联合干扰素栓治疗宫颈高危型HPV感染的效果

目的讨论三氯醋酸联合干扰素栓治疗宫颈高危型HPV感染的效果。方法选取我院于2011年4月~2014年3月收治的宫颈高危型HPV感染患者56例,将其随机分为观察组28例和对照组28例,我们对对照组采用三氯醋酸进行治疗,而对于观察组则在使用三氯醋酸治疗的基础上,联合干扰素栓进行治疗,进而观察两组患者治疗后的疗效分析。结果观察组患者的总有效率92.9%明显高于对照组的总有效率82.1%。结论使用三氯醋酸联合干扰素栓治疗宫颈高危型HPV感染疗效显著,且运用此种方法治疗患者的治疗过程中无需麻醉,并且无创伤及出血症状,操作简单,价格低廉,值得在临床上进行广泛的推广使用。     

HPV感染和p53异常表达与宫颈鳞癌的关系

目的研究宫颈鳞癌与p53蛋白表达和HPV感染的关系,探讨宫颈鳞癌的形成机制。方法采用RT-PCR法分别检测p53基因在39例宫颈鳞癌组织和39例正常宫颈黏膜组织中的表达情况以及PCR方法检测HPV在这些组织中的感染情况。结果 HPV在宫颈鳞癌组中阳性率为48.72%(19/39),正常组织中为14.81%(4/27),p53基因表达在宫颈鳞癌组中阳性率为53.85%(21/39),正常组织中为18.52%(5/27),宫颈癌组的HPV感染和p53表达均高于正常组(P0.05)。结论 HPV感染与p53基因的异常表达与宫颈鳞癌发生密切相关,联合检测能提高准确性。     

HPV感染各类宫颈疾病肿瘤标志物检测的临床价值

研究表明,人类乳头状瘤病毒（humanpapiUomavirus,HPV）感染为宫颈癌前病变和浸润性宫颈癌发生的必要条件。故及时监测HPV是预防、诊断和治疗宫颈病变和宫颈癌的必要检查项目。而肿瘤标志物在临床上主要应用于肿瘤的筛查、辅助诊断和预后判断。本文拟对HPV感染各类宫颈疾病患者肿瘤标志物进行测定和比较分析,探讨其与疾病变化的关系。     

宫颈高危型HPV感染对宿主细胞因子IL-18影响的实验研究

目的 检测高危型HPV感染宫颈上皮瘤样病变及宫颈癌患者外周血IL-18的水平变化，分析Th1细胞的细胞因子免疫活动，为肿瘤的免疫治疗提供实验依据。方法 在细胞因子中加入荧光标记的特异性抗细胞因子单克隆抗体，特异性抗原抗体结合，以流式细胞仪分析特异性细胞因子IL-18表达水平。结果 高危型HPV感染宫颈上皮瘤样病变及宫颈癌患者IL—18表达水平较正常对照组降低，差异有统计学意义。结论 高危型HPV感染宫颈上皮瘤样病变及宫颈癌宫颈癌患者IL-18表达降低，这可能是肿瘤细胞发生免疫逃逸，从而导致肿瘤的发生或者转移的原因之一。     

HPV感染和宫颈病变的相关性分析

目的研究北京地区女性HPV感染和宫颈病变关系。方法选择于2014年5月至2018年12月于民航总医院妇产科就诊行阴道镜并取活检的患者,按照宫颈活体病理结果分为无宫颈病变组,LSIL组,HSIL组,宫颈癌组,对其宫颈细胞学、HPV感染和病理结果进行相关性分析。结果共收集2634例患者,无病变组752例,LSIL组1348例,HSIL组497例,宫颈癌组37例。宫颈病变患者共1882例,占71.5%。宫颈鳞状上皮内瘤变及宫颈癌的的高发年龄为30-44岁。HSIL组、LSIL组和宫颈癌组最常见的感染型别均为HPV16型,LSIL组第二三位感染型别依次为HPV 52、HPV58,HSIL组第二三位感染型别依次为HPV 58、HPV52。第四位均为HPV18型;在宫颈癌组中HPV18感染居第二位,HPV52、HPV58、HPV33、HPV68并列位于第四位。结论符合入组条件者发生宫颈病变几率高,应及早行阴道镜检查,重视30-44岁女性的筛查。TCT的阳性结果比阴性结果更有意义,建议TCT与HPV联合筛查减少漏诊率(尤其是30-44岁女性)。除HPV16、18外,建议HPV52、58的感染及时转诊阴道镜检查。     

HPV感染与宫颈癌前病变

宫颈癌是一个由癌前病变逐渐衍变为癌的连续的病理过程。目前认为宫颈癌前病变,即宫颈上皮内瘤变与HPV感染有关。HPVs是一种双链小DNA病毒,由病毒蛋白外壳和核心DNA物质构成。病毒基因组分为早期基因区、晚期基因区及长调控区;其中早期区编码的E6、E7蛋白对于病毒的复制起关键作用。生殖道HPVs在有性活动的人群中普遍存在,在有性生活的女性中,至少有75%将在人生中的某个时间感染HPV。感染HPV后绝大多数人可以自然消退。只有感染了高危亚型、同时又具备其他高危因素的妇女才可能进展为HSIL或宫颈癌。因此对于HPV的感染既要重视,又不必恐惧。目前的治疗主要是针对由HPV引起的宫颈或外生殖器的局部病变。     

多重HPV感染与宫颈病变的临床分析

目的探讨多重人乳头状瘤病毒（HPV）感染与宫颈病变之间的关系。方法采用核酸分子快速导流杂交基因芯片技术,对2008年7月至2010年7月在东莞市太平人民医院就诊且有病理确诊的332例女性患者（实验组）及100例正常妇女（对照组）进行HPV基因分型检测,比较不同宫颈病变类型与HPV多重感染的关系。结果 332例宫颈病变中HPV感染率为78.61%（261/332）,多重感染率为58.13%（168/289）,其中以二重感染为主,最常见的二重感染类型为HPV16、58及HPV52、58,以HPV16型感染多见;多重HPV感染比例随病变级别增加逐渐上升,由对照组的17.86%升高到宫颈鳞癌组的100%,各病变组与对照组相比差异有统计学意义（P〈0.05）。结论 HPV多重感染与宫颈病变的发生有关,可作为宫颈病变早诊早治的有效指标之一。     

保妇康栓联合干扰素治疗宫颈柱状上皮异位合并HPV感染患者的临床疗效分析

目的:研究保妇康栓联合干扰素治疗宫颈柱状上皮异位合并人类乳头瘤病毒(Human papillomavirus,HPV)感染患者的临床疗效.方法:选取我院2018年11月～2021年1月收治的112例宫颈柱状上皮异位合HPV感染患者进行研究,按照随机双盲法将患者划分为对照组(n=56,保妇康栓治疗)和实验组(n=56,保...     

佛山和广州女性宫颈细胞HPV基因分型分析

目的 调查佛山和广州两个地区女性HPV病毒感染情况和分型特点。方法 选取2018年1月1日~12月31日广州和佛山送检医院在我检验中心进行检测的12571例由医院妇产科医生采集宫颈脱落细胞放入专用细胞保存液的取样管中送检标本,采用PCR-膜杂交法进行HPV基因分型检测,并分析其分布特征。结果 12571例标本中HPV阳性者2559例,感染率20.35%,佛山和广州的HPV阳性率分别为:27.63%、17.12%;两地HPV高危型别阳性率排名前5的型别均为:51、52、58、53、16;不同阶段年龄组比较HPV感染率差异有统计学意义,两地均在≤20岁组和＞50组阳性率高;两地女性HPV感染均以单一型别感染为主,单一型别感染占比佛山78.86%、广州72.94%。结论 佛山和广州两地的HPV感染情况大致相同:两地女性人群HPV感染均以单一型别感染为主,感染高危型别集中在51、52、58、53、16五个型别,低龄化人群阳性率高。有必要进行HPV感染的预防和定期筛查工作。     

HPV mRNA检测在宫颈癌诊断中的研究

根据国际癌症研究机构(IARC)报道,仅在2012年全世界就有超过25万女性死于宫颈癌,这是女性相关癌症的第四大死因[1].不管是生物学研究还是流行病学研究都已经证实宫颈癌与人乳头瘤病毒(HPV)感染息息相关[2]l.单独的HPV感染可能会引起大约5％的病毒相关的癌症发生,但是却是宫颈癌发病的全部因素[3].目前已经有120多种不同基因型的HPV,根据其临床表现可分为低风险型和高风险型,然而只有少数的HPV能够导致癌症的发生[4].本文就HPV致宫颈癌的发生和HPV的实验室检查以及HPV mRNA的相关进展做一些简要论述.     

Cervical HPV infection among HIV-infected prostitutes addicted to hard drugs

An investigation into the prevalence of human papilloma virus (HPV) infection, abnormal cervical cytology and the relationship between HIV-and HPV infection was done in a group of intravenously (IV) and non-IV drug-using prostitutes. From July 1991 through May 1992, hard drugaddicted prostitutes attending a sexually-transmitted-disease (STD) clinic in Amsterdam were recruited. A questionnaire was administered to obtain demographic characteristics, and medical and STD history. Apart from routine STD examination, cervical scrapes for cytology and samples for HPV DNA detection by polymerase chain reaction (PCR) were collected. Some of the women included in this study also participated in HIV studies among drug users. Their data on HIV- and immunologic status could be combined. A total of 121 women entered the study; 25 women were HIV-seropositive, 44 women were HIV-negative, and the HIV status of 52 women was unknown. All 25 HIV-positive women had normal Pap smears, two of the 44 HIV-negative women had a Pap smear III A, and in the HIV-unknown group, two women with Pap III A and one with Pap III B were found. Eight of the 25 (32%) HIV-positive women were HPV DNA-positive, three of the 44 (7%) HIV-negative women and 10/52 (19%) of the HIV-unknown group. Logistic regression analysis showed that in the total group, presence or cervical HPV DNA was associated with HIV infection (order ratio [OR] for HIV-positives 7.8, 95% confidence interval [CI] 1.8 to 34.6) and with diagnosis of condylomata acuminata at entry to the study (OR 7.5, 95% CI 1.5 to 36.5). The mean of the calculated minimal duration of HIV infection was 6.5 years for HPV-positive women vs. 4.2 years for the HPV-negative women (P = 0.001, OR 1.8 per year, 95% CI 1.1 to 3.2). The difference of CD4 T-cell counts between HPV-positive and negative women (all HIV-positive) was statistically not significant (557/mm³ vs. 486/mm³). Our data indicate that in this group of hard drug-addicted prostitutes, HIV infection is associated with a higher prevalence of HPV infection but not with a higher rate of abnormal cervical cytology. In the group of HIV-infected women, an association between CD4 T-cell counts and HPV infection was not established.     

300例妇科宫颈病变患者石蜡切片HPV基因分型结果分析

探讨女性生殖道感染人乳头瘤病毒(HPV)和不同宫颈病变的关系及HPV检测在宫颈癌防治方面的应用价值.对阴道镜活检病理诊断的300例不同的宫颈病变患者进行HPV-DNA检测(同时检测5种低危型和18种高危型HPV亚型).结果显示,慢性宫颈炎组HPV阳性87例,阴性48例,阳性率64.5%;子宫颈上皮内瘤变(CIN)组(C...     

Distribution of HPV genotypes in Shanghai women

Objective: To study the Distribution of HPV genotypes in Shanghai women. Design: Cohort study. Setting: Shanghai First Maternity and Infant Hospital affiliated with Tongji University. Population: Patients those attended in the cervical disease diagnosis and treatment center of Shanghai First Maternity and Infant Hospital between January 2011 and December 2014. Methods: HPV GenoArray test kit (HybriBio Ltd) was used to perform HPV genotyping and was also used in DNA amplification and HybriBio’s proprietary flow-through hybridization technique. Results: In this study, total patients analyzed were 4585. Among 4585 sample the HPV positive patients were 1460 i.e. 31.84% in total. On the basis of pathological report normal were 1358, with inflammation 2441, with low grade lesion were 399, high grade lesion were 353, CIN were 19 and cervical carcinoma were 15. Among normal HPV positive were 215 (15.8%), among inflammation HPV positive were 735 (30.11%). HPV positive in low grade lesion were 353 i.e. 59.77%. In high grade lesion 211 were HPV positive among 272 (68.17%). The percentage of HPV positive was 73.68% i.e. 14 out of 19 patient in cervical carcinoma in situ. 13 patient out of 15 i.e. 86.67% of Cervical carcinoma were HPV positive. Among all percentage of HPV positive was high among cervical carcinoma then cervical carcinoma in situ then high grade lesion in decreasing fashion to low grade lesion and in normal. Highest prevalence i.e. 22.67% is of HPV 52 subtype and HPV 16 has second highest prevalence with 17.67% among HPV positive cases. Sensitivity of TCT detection is 71.6%. Specificity of TCT detection is 79.6%. Sensitivity of HPV-DNA detection is 65.2%. Specificity of HPV-DNA detection is 78.2%. Conclusion: HPV is one of major health concern in shanghai having high prevalence rate in comparison to other part of china and other part of world. This has implications for the future cervical cancer burden and the priority to be given to prevent cervical cancer in Shanghai, especially, given the promising efficacy of prophylactic vaccines against HPV52, 16 and 58. This study also shows high sensitivity and specificity of TCT and HPV-DNA detection.     

宫颈癌相关基因表达与HPV感染的相关性研究

目的 探讨宫颈癌凋亡相关Bcl-2、TRAIL及Caspasc-3在宫颈组织中的表达及与人乳头瘤病毒感染(HPV)的相关性.方法 采用免疫组织化学方法对宫颈正常组织(NE)26例,宫颈鳞状细胞癌组织(SCC) 30例进行Bcl-2、TRAIL及Caspasc-3蛋白表达的测定,同时采用PCR技术测定各组患者HP16/18感染情况.结果 ①TRALL在SCC组宫颈活检组织中阳性表达率显著低于NE组(P＜0.05),SCC组中Caspage-3阳性表达率均显著低于NE组(P＜0.05),Bcl-2、HPV16/18在SCC组宫颈活检组织中阳性表达率显著高于NE组,(P＜0.05).②在宫颈组织中,TRALL阳性表达率与组织分化程度具有相关性(P＜0.05),Caspage-3阳性表达率与组织分化程度及临床分期具有相关性(P ＜0.05);Bcl-2阳性表达率与组织分化程度及临床分期具有相关性(P＜ 0.05);HPV16/18阳性表达率和SCC病理特征均无关.③HPV16/18感染及Bcl-2表达与TRALL、Caspage-3表达呈负相关(P＜ 0.05);TRALL与Caspage-3表达呈正相关(P＜0.05),HPV16/18感染与Bcl-2表达呈正相关(P＜0.05).结论 Bcl-2的过度表达,TRAIL及Caspasc-3低表达和HPV16/18感染在宫颈癌发病过程中发挥协同作用.     

HIV合并HPV阳性标本中HPV的型别检测

目的探讨深圳地区人类免疫缺陷病毒（HIV）阳性人群中人乳头瘤病毒（HPV）感染分型情况,为HIV阳性人群中HPV感染的防治提供依据。方法运用荧光PCR方法和反向斑点杂交技术对HPV阳性患者进行HPV分型检测。结果在HIV感染者中。利用反向点杂交分型方法对40例HPV阳性标本进行HPV基因分型。其中单型感染有18例（45．0％）,混合感染有19例（47．5％）,共检出16种HPV型别,其中包括11种高危型（16、18、31、33、35、45、52、56、58、68、73型）和5种低危型（6、11、42、44、54型）。在16种型别中,感染率最高的为16型（25．0％）,其次为52型（17．5％）、58型（15．0％）。结论感染HIV人群中生殖器部位HPV的感染率较高,在HIV阳性人群中检出性病相关的高危HPV16／52／58亚型．对HIV阳性人群中HPV感染的防治有重要指导意义。     

The relationship between the cervical and anal HPV infection in women with cervical intraepithelial neoplasia

BackgroundMore than 90% of cases of anal cancers are caused by high-risk human papillomavirus (HR HPV) infection and a history of cervical intraepithelial neoplasia (CIN) is established as possible risk factor.ObjectivesTo demonstrate relationship between anal and cervical HPV infection in women with different grades of CIN and microinvasive cervical cancer.Study designA total of 272 women were enrolled in the study. The study group included 172 women who underwent conization for high-grade CIN or microinvasive cervical cancer. The control group consisted of 100 women with non-neoplastic gynecologic diseases or biopsy-confirmed CIN 1. All participants completed a questionnaire detailing their medical history and sexual risk factors and were subjected to anal and cervical HPV genotyping using Cobas and Lynear array HPV test.ResultsCervical, anal, and concurrent cervical and anal HPV infections were detected in 82.6%, 48.3% and 42.4% of women in the study group, and in 28.0%, 26.0% and 8.0% of women in the control group, respectively. The prevalence of the HR HPV genotypes was higher in the study group and significantly increased with the severity of cervical lesion. Concurrent infections of the cervix and anus occurred 5.3-fold more often in the study group than in the control group. Any contact with the anus was the only significant risk factor for development of concurrent HPV infection.ConclusionsConcurrent anal and cervical HR HPV infection was found in nearly half of women with CIN 2+. The dominant genotype found in both anatomical locations was HPV 16. Any frequency and any type of contact with the anus were shown as the most important risk factor for concurrent HPV infection.     

HPV分型检测联合TCT检查在宫颈病变筛查中的应用

目的 探究人乳头瘤病毒（HPV）以及液基细胞学（TCT）的联合检测在宫颈病变的筛查中的应用。方法 选取2015 年12月~2016年11月因宫颈异常来我院就诊的2327例妇科疾病患者,对其实施TCT以及HPV DNA检测,对阳性的患者进行阴道镜活检,以患者组织的病理学结果作为最终确诊标准。结果 共计355例患者进行阴道镜检查,其中HPV检测灵敏度为75.72%,准确性为70.43%,特异性为30.22%;TCT检测灵敏度为41.32%,准确性43.40%,特异性为47.87%;二者联合诊断灵敏度为88.21%,准确率75.10%,特异性为43.36%。两种检测方法的阳性检出敏感度相比,差异有统计学意义（P＜0.05）。结论 HPV分型检测联合TCT的检测方式具有相当高的阳性率及灵敏度,能够提高患者宫颈病变的检出率,延长患者的筛查间隔,有利于临床上对于宫颈病变的筛查。