128例原发性不育患者精液解脲脲原体的检测

采用解脲脲原体检测试剂对１２８例原发性不育患者的精液进行解脲脲原体病原学诊断，阳性率为３１．２５％（４０／１２８）。本文结果提示在山西省大同地区，解脲脲原体感染可能是引起男性原发性不育的重要原因之一。     

抗解脲脲原体单克隆抗体的制备

作者用提纯的解脲脲原体抗原免疫BALB/c小鼠,取其脾细胞,与小鼠骨髓瘤细胞FO融合,获得3株稳定分泌抗解脲脲原体单克隆抗体的杂交瘤细胞株。制备了小鼠腹水抗体,并对杂交瘤细胞株进行了鉴定。用ELISA法测定该抗体腹水效价为1:64000～1:2048000,1F9、2B2抗体的Ig类型为IgG1,5H1为IgG2b。建立的3株能稳定分泌抗解脲脲原体的McAb杂交瘤细胞林,经液氮冻存后复苏,其分泌抗体能力不受影响。     

妇科疾病中的解脲脲原体、沙眼衣原体Nesed-PCR检测

目的探讨UU、CT在本地区妇科疾病中的发病率及其致病作用,方法收集535例妇科阴道炎、不孕症、宫颈炎、附件炎等疾病患者的阴道分泌物,应用nested PCR(nPCR)技术检测解脲脲原体(UU)及沙眼衣原体(CT)核酸.结果393人检测解脲脲原体(UU),UU-DNA阳性192例(48.8%);142人检测沙眼衣原体(CT),CT-DNA阳性32例(22.5%).结论解脲脲原体(UU-DNA)、沙眼衣原体(CT-DNA)与妇科疾病的发生关系密切,特别是阴道炎的妇科患者.     

RAPD基因指纹图谱在解脲脲原体分型研究中的应用…

采用随机扩增多态性ＤＮＡ（ＲＡＰＤ）技术，以美国Ｏｐｅｒｏｎ公司生产的ＯＰＬ１￣２０十本脱氧寡核苷酸为引物，对解脲脲原体８和１０血清型标准株基因指纹图谱进行了构建。通过比较发现，两型解脲脲原体ＤＮＡ间存在同源性和多态性。初步研究结果表明，ＲＡＰＤ可用于解脲脲原体分型。     

解脲脲原体感染与先兆流产关系的探讨

解脲脲原体 (Ｕｒｅａｐｌａｓｍａｕｒｅａｌｙｔｉｃｕｍ ,Ｕｕ)是引起生殖道感染的主要支原体 ,解脲脲原体感染可导致不良的妊娠结局[1] 。有关解脲脲原体与自然流产关系的报道[2 -4 ] 己引起了人们的重视 ,为了进一步探讨Ｕｕ与流产的关系 ,本文对10 7例先兆流产患者的宫颈分泌物进行了解脲脲原体的选择培养 ,并与对照组进行了对比分析。资料与方法一、检测对象　检测组为 10 7例早孕期有先兆流产症状(阴道流血、下腹疼痛等 )要求保胎患者 ,年龄 2 1- 42岁 ,平均年龄 (2 8.9± 3.5 )岁 :对照组为 2 8例正常早孕者 ,年龄 2 2- 40岁 ,平均…     

RAPD基因指纹图谱在解脲脲原体分型研究中的应用初探

采用随机扩增多态性DNA(RAPD)技术,以美国Operon公司生产的OPL1～20十聚体脱氧寡核苷酸为引物,对解脲脲原体8和10血清型标准株基因指纹图谱进行了构建。通过比较发现,两型解脲脲原体DNA间存在同源性和多态性。初步研究结果表明,RAPD可用于解脲脲原体分型。     

妇科病人沙眼衣原体,解脲脲原体淋球菌感染的流行病学研究

本文磁式ＰＣＲ科病人的沙眼衣原体、解脲脲原体、解脲脲原体、淋球菌感染率。结果阳性率分别为１５％、２０．９％、３．８％。１２０例阳性病例的男性配偶阳性率分别为１５％、３０％、２．５％。     

宫内解脲脲原体和人型支原体感染与自发早产发生的关系及其对早产儿的影响

目的 检测胎龄23～32周早产儿脐带血中解脲脲原体、人型支原体的感染率和脐带血IL-6阳性率,并判断其与胎膜早破以及早产儿预后的相关性.方法 2009年1月-2010年1月于新乡市中心医院出生的187例早产儿和53例足月儿及其母亲入选本实验,利用PCR技术测定脐带血解脲脲原体和人型支原体,利用放射免疫法测定脐带血IL-6;搜集并记录患儿母亲相关医学信息;对入选早产儿进行预后追踪.结果 自发早产儿与非自发早产儿脐带血IL-6阳性率以及解脲脲原体和/或人型支原体检测阳性率比较,差异有统计学意义(P=0.005,P=0.009);胎膜早破与解脲脲原体和/或人型支原体感染相关(P=0.006).解脲脲原体和/或人型支原体感染与早产儿全身炎症反应综合征(SIRS)和支气管肺发育不良(BPD)的发生相关(P=0.017,P=0.031);而与早产儿呼吸窘迫综合征(RDS)无关(P=0.116).结论 解脲脲原体和/或人型支原体感染是自发早产发生的一个重要原因,并影响早产儿的预后.     

解脲脲原体的实验室检测方法的研究现状

解脲脲原体是一种条件致病菌，多与宿主共存，仅在某些条件下引起机会性感染，感染部位常为男女泌尿道，其中更易致病的为U．urealyticum型。近年来检测解脲脲原体的方法主要有液体培养法、固体培养法、免疫学方法和分子生物学方法等等，这些检测方法各有优劣。本文就临床使用较多的液体培养法、固体培养法和分子生物学方法进行重点分析，希望能给临床提供更多的帮助。     

精索静脉曲张及解脲脲原体感染与不育症关系探讨

本文观察了250例精索静脉曲张伴不育者。患者年龄21－40岁，左侧精索静脉曲张236例，右侧11例，双侧3例。对确诊为精索静脉曲张患者的精液进行常规检测及解脲脲原体培养，结果少精症129例，精子无力症121例，解脲脲原体阳性者132例（52．80％）。我们认为精索静脉曲张引起局部血液循环减缓，代谢产物积聚、温度升高，组织缺氧等变化，导致精子数目减少，活动度减低。同时伴有解脲脲原体感染者，亦可引起精子畸形及其功能下降，由于这些因素从而造成不育症。文中讨论了治疗有关问题。     

继发不育男性泌尿生殖道支原体感染状况与耐药性分析及其临床应用研究

目的探讨继发不育男性患者泌尿生殖系支原体的感染状况及其耐药性。方法采用支原体分离培养鉴定与药敏试剂盒，对继发不育男性患者泌尿生殖系分泌物进行支原体分离培养鉴定与药敏测试。结果572例继发不育男性患者，泌尿生殖系支原体培养阳性278例，阳性率为48．6％，其中Uu占90．3％（251／278）；Mh占1．4％（4／278）；Uu合并Mh占8．3％（23／278）。药敏试验表明：喹诺酮类耐药率最高，四环素类和大环内酯类耐药率最低。结论支原体感染是男性继发不育的重要危险因素，在支原体感染的治疗过程中，我们应根据药敏测试结果选择敏感抗生素。     

MIC 基因多态性与解脲脲原体感染的易感关联性研究

目的:研究MIC 等位基因多态性与解脲脲原体感染易感性之间的关联性。方法:采用PCR-SSP 和PCR-SBT方法对样本MIC 等位基因的多态性进行检测。结果:解脲脲原体患者中检出12 种MICA、5 种MICA-STR 和14 种MICB 等位基因;和对照组相比较,解脲脲原体患者组中MICA* 010 和MICB*009N 等位基因分布频率更高(MICA*010:OR=3.85,95%CI:2.12-6.99,Pc<0.05;MICB*009N:OR=3.22,95% CI:1.33-7.80,Pc<0.05),MICA-A5.1 和MICB*00502 等位基因分布频率更低(MICA-A5.1:OR=0.61,95%CI:0.40-0.94,Pc<0.05;MICB*00502:OR=0.58,95%CI:0.40-0.83,Pc<0.05),差异均具有统计学意义;基因型方面,MICA*010/010、MICA*01201/01201 纯合子分布频率更高(MICA*010/010:OR = 14.84,95%CI:1.90-115.9,Pc<0.05;MICA*01201/01201:OR=10.83,95% CI:1.35-86.79,Pc<0.05),差异均具有统计学意义;解脲脲原体患者中MICB*00502/00502 纯合子分布频率较高,但差异不具有统计学意义(Pc>0.05)。结论:MIC 等位基因多态性与解脲脲原体的易感性间存在关联性。     

原因不明不孕症妇女月经周期与解脲脲原体感染关系的探讨

本文检查了７４例不明原因的不孕症妇女生殖道解脲脲原体感染的情况，其中３９例阳性，正常组５１例１４例阳性，两组比较有显著性差异。同时观察到解脲脲有原体检出率的高低与月经周期有关，排卵前７天至排卵后８天不孕症组的阳性率为６３％，明显高于卵泡早期２３％和黄体晚期２８．５７％。     

Ureaplasma in lung. 2. Association with bronchopulmonary dysplasia in premature newborns

Infants with Ureaplasma urealyticum in the lower respiratory tract are at risk for chronic lung disease (CLD) or bronchopulmonary dysplasia (BPD) but causality has been difficult to prove. The goal of this study was to identify ureaplasma in human neonatal lung tissue using the in situ hybridization (ISH) procedure described in Part 1 (Exp. Mol. Pathol., in press) of this report. By correlating their presence with the histopathologic findings, it may be possible to provide further evidence of the pathogenicity of ureaplasmas and their association with BPD. Lung autopsy tissue from seven infants with positive cultures and seven infants with negative cultures for ureaplasma were included in the study. All culture-positive infants were positive for ureaplasma on ISH and all had histopathologic evidence of BPD. Two of the seven infants with negative cultures were positive for ureaplasma with ISH. Of interest, these two infants were also found to have BPD at autopsy. The other five infants with negative cultures were also negative for ureaplasma on ISH and had no evidence of BPD. This study correlates the presence of U. urealyticum by ISH with the finding of BPD on histopathologic evaluation and provides evidence that it has a role in the development of CLD.     

Hemadsorption by colonies of Ureaplasma urealyticum.

Hemadsorption by colonies of Ureaplasma urealyticum and Mycoplasma pneumoniae differed quantitatively and qualitatively. Using standard methodology, few strains of U. urealyticum hemadsorbed; with a modified method, most strains hemadsorbed, indicating a second type of association. Scanning electron microscopy of tannin-osmium-stained preparations showed guinea pig erythrocytes embedded in ureaplasma colonies and craters left when erythrocytes were dislodged.     

男性不育症患者精液标本的病原学调查

目的了解我院近三年来男性不育症患者精液标本的细菌菌谱分布及衣原体、支原体的感染情况,为临床治疗用药提供依据。方法对2004年～2006年间来我院生殖中心就诊的不同病因的3533例男性不育症患者的精液标本病原体培养的结果进行统计分析,分析其致病菌的分布及耐药情况。结果 2004、2005、2006年的413、1303、1817份男性不育症患者的精液标本中分别有195、650、795份病原体培养阳性。检到细菌分别为145、498、639株,以革兰阳性菌为主,2004、2005、2006年分别占89.7%(130/145)、89.6%(446/498)、93.1%(595/639)。革兰阳性菌中又以表皮葡萄球菌为主,近三年分别占43.1%(56/130)、37.8%(170/450)、31.1%(185/595);分离到的表皮葡萄球菌对万古霉素全部敏感;对苯唑西林耐药率最高。近三年分别有18、124、66份精液标本衣原体金标试验阳性;分别有119、304、408份精液标本检到支原体,检到的支原体以解脲支原体(UU)为主,解脲支原体对氧氟沙星耐药率最高、交沙霉素耐药率最低。结论我院近三年男性不育症患者的精液标本很大部分可分离到细菌及支原体和衣原体,表明感染是引起男性不育的一个重要因素。病原菌主要以革兰阳性菌、解脲支原体为主;致病菌多样化,使得对不育症患者的治疗复杂化。对男性不育症患者的治疗需加强病原菌的检测和其耐药性的分析,进行合理用药,提高不育症患者的生育率。     

Cloning of Ureaplasma urealyticum DNA sequences showing genetic homology with urease genes from gram-negative bacteria

DNA chromosomic sequences from Ureaplasma urealyticum were shown, by DNA hybridization, to possess homology with Providencia stuartii urease genes. The homologous ureaplasma DNA fragment was cloned in Escherichia coli and a DNA probe, designated IC61 probe was specific for only the urease-producing U. urealyticum among the Mollicutes. Genetic homology shown between the IC61 probe and urease genes from Campylobacter pylori, E. coli and P. stuartii suggests that (1) the cloned DNA fragment from U. urealyticum contains urease encoding sequences, and that (2) the urease genes possess conserved sequences among phylogenetically distant prokaryotes.     

解脲支原体感染与早产儿呼吸系统疾病的相关性

目的探讨解脲支原体定植在早产儿肺部疾病中的作用。方法对胎齢小于32w的行机械通气的早产儿的气道吸引物进行培养,查找分离解脲支原体,并对阳性组和阴性组进行对照分析。结果143个病例中有39例(27%)分离培养阳性。阳性者呼吸窘迫综合征发生率明显低于阴性者(P=0.002)。多元回归分析显示,在单胎儿中,只有解脲支原体定植是呼吸窘迫症(RDS)的独立预测因子(OR=0.38;P=0.02),胎龄(OR=0.47;P=0.006)和解脲支原体阳性(OR=3.1;P=0.05)都是慢性肺部疾病(CLD)的独立预测因子,这里的CLD是按新法定义的:纠正胎龄达到36周仍需要氧疗。结论解脲支原体气道内定植能预防早产儿发生RDS,但却使早产儿CLD的发病率增加。     

Ureaplasma in lung. 1. Localization by in situ hybridization in a mouse model

Ureaplasma urealyticum is a common inhabitant of mucosal surfaces but is also associated with a higher incidence of pneumonia and bronchopulmonary dysplasia in preterm infants. Culture and polymerase chain reaction demonstrate high isolation rates of ureaplasma in clinical specimens documenting their presence but do not associate the organism directly with the diseased tissue. In this study, lung tissue samples from newborn mice inoculated intranasally with U. urealyticum were used to develop an in situ hybridization (ISH) test for the organism. In situ hybridization allows the localization of gene expression for visualization within the context of tissue morphology. New techniques which use biotinyl-tyramide based signal amplification have been able to greatly enhance the sensitivity of ISH. Using the Dako GenPoint Catalyzed Signal Amplification system to detect a biotinylated DNA probe specific for an internal nucleotide sequence within the urease gene of U. urealyticum, the organism was detected within the infected murine lung tissues. Electron microscopy was used to verify the presence of the organisms in the positive ISH areas. The ISH procedure developed in this study can be used to analyze the presence of ureaplasma in human neonatal lung tissue with the corresponding histopathology.     

Modified indirect immunofluorescence test for serotyping large numbers of Ureaplasma urealyticum clinical isolates.

A technical modification of the indirect immunofluorescence test for serotyping Ureaplasma urealyticum clinical isolates is described. The use of a tissue culture plate filled with ureaplasma agar made the serotyping easier to perform and proved to be very handy, especially for performance of large series.