宜城人民医院门诊1200例体检妇女HPV感染情况及相关因素分析

目的：检测并分析宜城人民医院妇科门诊行妇科检查妇女宫颈人乳头瘤状病毒(human papilloma virus，HPV)的感染率及高危因素。方法：选取本院妇科门诊进行妇科检查的1200例妇女作为调查对象，采用实时荧光定量PCR法(FQ-PCR)测定1200例妇女宫颈脱落细胞HPV感染率及分型情况，并收集调查对象的一般资料、行为信息学指标，采用单因素和多因素分析HPV感染的高危因素。结果：1200例调查对象，收集到检测样本及调查资料符合要求的共有1184例，其中共计检出HPV阳性感染者104例，阳性感染率为8.78%；HPV16感染阳性率占总感染患者的38.46%，其次为HPV58达到29.81%；多因素结果显示，性伴侣数目>1个(OR=1.721)、性生活频次>4次/月(OR=1.583)、宫颈糜烂(OR=2.081)是中青年妇女发生HPV感染的主要高危因素。结论：中青年妇女HPV感染的主要型别为HPV16，主要危险因素为多性伴侣、性生活较频繁、宫颈糜烂。     

新疆维吾尔族患者HPV感染型别临床分析

目的分析新疆维吾尔族妇女子宫颈HPV感染类型及其分布的规律。方法采用导流杂交基因分型技术对318名妇科维吾尔族就诊患者进行HPV基因分型检测。结果 HPV-DNA检测阳性者占21.70%（69/318）。低危型感染12例（20.69%）,以HPV 6和HPV 1l为主;高危型感染46例（79.31%）,以HPV 16、HPV58和HPV68感染为主。多重感染11例,感染率为3.46%。从年龄分布来看,≤25岁的患者占31.48%,HPV感染率最高,其次为50~55岁年龄段的患者占25.71%。结论 HPV分布存在地域和种族差异,加强对新疆各年龄人群,多种型别HPV的筛查,有助于预防宫颈癌的发生及了解HPV感染的转归,并为新疆地区宫颈癌干预提供理论基础。     

杭州地区性病门诊男性就诊者HPV感染的流行病学调查

目的 了解杭州地区性病（STD）门诊男性就诊者中人乳头瘤病毒（HPV）感染的流行情况。方法 利用HPV黏膜型通用引物MY09／11。PCR方法检测STD门诊年龄在18—70岁男性就诊者阴茎拭子中的HPV DNA，并对阳性PCR产物用限制性长度片段多态性方法和直接测序法确定病毒基因型。结果 在采集的375例阴茎拭子中，81．3％（305例）产生足够的DNA可用于PCR扩增。HPVDNA的阳性率为13．8％（42／305），其中低危型HPV的阳性率为8．5％（26／305），高危型HPV的阳性率为4．3％（13/305），混合型感染的阳性率为1．0％（3/305）。居住地在城市、受教育程度越低、性伴侣的人数越多HPV DNA的检出率越高（P〈0．05）。结论 HPV在男性高危人群的感染并不少见，HPV DNA的检出与就诊者的居住地、教育程度及性伴侣人数有相关性。对男性高危人群HPV的感染应引起重视，积极采取预防和控制措施。     

2016~2018年苏州市育龄妇女HPV感染因素分析

目的 调查苏州市育龄妇女人乳头状瘤病毒（HPV）感染情况及其影响因素。方法 以整群抽样方式选取2016年7月~2018年12月苏州市育龄妇女4275例为研究对象,进行HPV和TCT检查,并填写调查问卷,了解HPV感染情况。结果 4275例孕妇中存在HPV感染者964例（22.55%）;HPV发生与年龄、职业、在苏时间、文化程度、每周性生活频率、性伴侣数、避孕方式、性传播疾病史、CIN、吸烟等因素有关,差异有统计学意义（P＜0.05）;Logistic回归分析显示:文化程度、性生活频率、避孕方式、吸烟、年龄、职业是苏州市育龄妇女HPV发生的独立危险因素（P＜0.05）。结论 苏州市育龄妇女HPV感染率较高,文化程度、性生活频率、避孕方式、吸烟、年龄、职业是其发生的独立危险因素,需加强对育龄期女性HPV感染知识宣教,增强认知,定期体检,早发现、早治疗。     

天津市1 902名患者人乳头瘤病毒感染特征及266名患者治疗情况分析

目的分析天津市1 902名患者的人乳头瘤病毒(human papillomavirus, HPV)感染特征及其中266名患者药物治疗情况, 以期为HPV感染的治疗提供理论参考。方法 2019年10月至2021年5月, 采用聚合酶链反应-反向斑点杂交技术对我院性病门诊1 902例15～86岁的患者进行HPV型别检测, 并对其中266名患者的药物治疗情况进行回顾性分析。结果 1 902名患者中HPV总感染率高达53.84%(1 024/1 902), 其中男性感染率为52.60%(689/1 310), 女性感染率为56.59%(335/592), 男性和女性感染率差异无统计学意义(P>0.05)。男性和女性中感染率最高的5种HPV基因型依次是HPV6(15.27%, 200/1 310和21.96%, 130/592)、HPV16(10.61%, 139/1 310和9.46%, 56/592)、HPV11(9.31%, 122/1 310和8.61%, 51/592)、HPV52(6.79%, 89/1 310和8.95%, 53/592)以及HPV43(5.64%, 87/1 ...     

多重HPV感染与宫颈病变的临床分析

目的探讨多重人乳头状瘤病毒（HPV）感染与宫颈病变之间的关系。方法采用核酸分子快速导流杂交基因芯片技术,对2008年7月至2010年7月在东莞市太平人民医院就诊且有病理确诊的332例女性患者（实验组）及100例正常妇女（对照组）进行HPV基因分型检测,比较不同宫颈病变类型与HPV多重感染的关系。结果 332例宫颈病变中HPV感染率为78.61%（261/332）,多重感染率为58.13%（168/289）,其中以二重感染为主,最常见的二重感染类型为HPV16、58及HPV52、58,以HPV16型感染多见;多重HPV感染比例随病变级别增加逐渐上升,由对照组的17.86%升高到宫颈鳞癌组的100%,各病变组与对照组相比差异有统计学意义（P〈0.05）。结论 HPV多重感染与宫颈病变的发生有关,可作为宫颈病变早诊早治的有效指标之一。     

男性HPV感染对精液参数的影响

目的探讨男性HPV感染对精液的影响。方法运用流式荧光杂交法,检测可育及不育男性的精液质量及其HPV感染情况。结果 638名受试者中HPV阳性共81例(12.70%)。353名不育男性中有64例HPV阳性(18.13%),其中多重感染15例;285名确切可育男性中有HPV阳性17例(5.96%),其中多重感染2例。相对确切可育男性,不育男性的精液有较高的HPV感染率;且HPV阳性精液的精子前向活力及精子形态率下降明显(P0.01)。结论 HPV感染可以降低精液质量,是男性不育的危险因素之一。     

三明地区人乳头瘤病毒（HPV）感染FQ-PCR检测与宫颈病变的关系

目的通过对三明地区妇女感染HPV情况的调查,为三明地区妇女感染HPV的预防提供科学依据。方法采用聚合酶多重核酸扩增荧光检测方法检测女性宫颈口脱落细胞进行13种高危型,5种低危型人乳头瘤状病毒（HPV）检测和分型。结果在129例正常组女性宫颈口脱落细胞标本中,HPV高危型和低危型阳性总检出率为29.5%（38/129）,其中高危型检出率为31%,低危型为69%。421例各种不同宫颈病变总检出率为89.3%（376/421）。其中宫颈炎症高危型检出率52%,低危型为48%;CINⅠ高危型为74%,低危型为26%;CIN Ⅱ高危型为84%,低危型为16%;CIN Ⅲ高危型为92%,低危型为8%;宫颈鳞癌高危型为100%;宫颈腺癌高危型为100%;宫颈湿疣高危型为94%,低危型为6%。结论随着宫颈病变的加重,CIN级别的增加,HPV感染率上升,在较高级别的宫颈病变中,大多为高危型HPV感染。     

义安区体检HPV-DNA筛查结果分析

目的 分析铜陵市义安区高危型HPV-DNA筛查结果,探讨HPV-DNA的临床价值。方法 对2016年3月~2016年5月义安地区1165例21~65岁妇女采用HPV-DNA检测筛查。结果 1165例标本中HPV-DNA阳性率为8.07%,共检测15种基因型,其中HPV52基因型感染率最高为1.89%,HPV11基因型感染率最低为0.09%,HPV单一感染发生率6.70%,二重HPV感染率0.94%,三重HPV感染率0.43%。结论 定期进行HPV-DNA检测,可以预测发生宫颈癌的风险,HPV分型检测对宫颈癌筛查具有重要的临床价值。     

自然流产妇女风疹病毒近期感染的检测

目的为了解自然流产妇女风疹病毒(RV)近期感染状况。方法采用酶联免疫吸附试验(ELISA)对1820名自然流产妇女和128名孕前检查的健康育龄妇女静脉血标本进行了RV-IgM检测。结果在1820名上述自然流产妇女中检测出RV-IgM阳性标本41例,阳性率为1.65%,在128例健康育龄妇女中未检测到RV-IgM阳性标本。结论在自然流产妇女中风疹病毒有较高的近期感染率。     

厦门地区女性人乳头瘤病毒感染状况调查

目的 对厦门地区妇女宫颈人类乳头瘤病毒（human papilloma virus,HPV）亚型进行筛查,以探讨其分布规律.方法 采用凯普医用核酸分子快速杂交仪,对7683名2013年1月-2013年12月到厦门市174医院妇科门诊或病房就诊的女性进行生殖道21种HPV感染基因亚型筛查.结果 7683例样本中,HPV感染者1421例,阳性率18.50％.感染人群主要集中在30～39岁.其中高危型HPV阳性率为16.01％,低危型HPV阳性率为2.49％.单一基因型别者1148例,阳性率为14.94％;双重感染者220例阳性率为2.86％;三重以上感染者53例,阳性率为0.70％;HPV感染阳性率居前6位的基因型分别为HPV-52（3.64％）、HPV-16 （3.33％）、HPV-58（2.98％）、HPV-53（2.16％）、HPV-CP8304（1.91％）和HPV-18（1.09％）.结论 厦门地区妇女HPV感染率高,且以高危型单一基因亚型感染为主;感染基因型别主要以HPV-52、16、58、53、CP8304和18为主,具有一定的地域差异性;52及58型感染率高,对于疫苗的研制和开发有参考意义.     

Prevalence of antibodies to human papillomavirus (HPV) type 16 virus-like particles in relation to cervical HPV infection among college women.

A human papillomavirus type 16 (HPV-16) virus-like particle (VLP)-based enzyme-linked immunosorbent assay (ELISA) was used to measure serum antibody to capsid proteins in 376 sexually active college women who were also screened for the presence of genital HPVs by PCR and interviewed for demographic and behavioral risk factors for HPV infection. The seroprevalence was 46% in women with HPV-16 DNA in the genital tract. The corresponding values for women who harbored other HPV types or no HPV in the genital tract were 30 and 19%, respectively (HPV-16 group versus no-HPV group; odds ratio [OR], 3.7; 95% confidence interval [CI], 1.5 to 8.9). The antibody response was significantly higher among women with a high viral load than among those with a low viral load (median optical density value, 0.838 versus 0.137, P = 0.009). Comparable levels of seroreactivity were observed among women infected with HPV types distantly or closely related genetically to HPV-16. Seroreactivity was significantly associated with an age of 25 to 30 years (OR, 2.3; 95% CI, 1.2 to 4.4), three or more lifetime sexual partners (OR, 2.9; 95% CI, 1.1 to 10), and history of a sexually transmitted disease other than HPV (OR, 3.1; 95% CI, 1.5 to 6.3). The percent seropositivity increased linearly with number of lifetime sexual partners until reaching a plateau at 35% for women with more than six partners (chi for linear trend, P < 0.001). The low sensitivity of HPV-16 VLP-based ELISA may limit the usefulness of the assay as a diagnostic test for HPV-16 infection. However, the assay appears to have adequate specificity and should be useful as an epidemiological marker of HPV-16 infection and sexual behavior.     

渝东南妇科女人乳头瘤病毒感染状况分析

目的:了解渝东南妇女人乳头瘤病毒(HPV)感染的基因型分布特点,为该片区HPV疫苗的研发和宫颈癌的防治提供可靠而科学的依据。方法:采用聚合酶链反应(PCR)和脱氧核糖核酸(DNA)反向点杂交相结合的DNA芯片技术对来自渝东南门诊妇科的5073例就诊者进行HPV感染及分型检测。结果:HPV总感染率为37.5%(1902/5073),其中单纯低危型感染率为6.8%(344/5073),以HPV6,81为主;高危型及混合感染率为30.7%(1558/5073),以其高危型检出频次前五位依次为16(21.9%),52(18.4%),58(17.6%),33(12.5%),18(9.1%)。共检出22种基因型,没检出82型;在各年龄组中,其感染率与年龄段呈U型曲线关系,高危型感染在50岁以上明显上升(X2=15.78,P0.005)。结论:HPV感染率及基因分型有一定的地域差异,该片区感染率较高,高危型及多重感染情况比较严重,基因分型符合亚洲人的特点,但也有各自特点。     

Epidemiology and risk factors for human papillomavirus infection in a diverse sample of low-income young women

BackgroundTwo HPV vaccines prevent infection with HPV-16 and HPV-18, high-risk (cancer-associated) HPV types which together cause approximately 70% of cervical cancers; one vaccine also prevents HPV-6 and HPV-11, which together cause approximately 90% of anogenital warts. Defining type-specific HPV epidemiology in sexually experienced women will help estimate the potential clinical benefits of vaccinating this population.ObjectivesTo examine HPV epidemiology in a diverse sample of sexually experienced women, and to determine factors associated with high-risk HPV and vaccine-type HPV (HPV-6, HPV-11, HPV-16 and HPV-18).Study designCross-sectional study of 13–26-year-old women (N = 409) who completed a questionnaire and provided a cervicovaginal swab. Swabs were genotyped for HPV using PCR amplification. Logistic regression models were used to determine whether participant characteristics, knowledge, and behaviors were associated with high-risk and vaccine-type HPV.ResultsMost women (68.4%) were positive for ≥1 HPV type, 59.5% were positive for ≥1 high-risk type, 33.1% were positive for ≥1 vaccine-type HPV, and 3.5% were positive for both HPV-16 and HPV-18: none was positive for all four vaccine types. In adjusted logistic regression models, Black race (OR 2.03, 95% CI 1.21–3.41) and lifetime number of male sexual partners (OR 4.79, 95% CI 2.04–11.23 for ≥10 partner vs. ≤1 partner) were independently associated with high-risk HPV infection.ConclusionsHPV prevalence was very high in this sample of sexually active young women, but <5% were positive for both HPV-16 and HPV-18, suggesting that vaccination could be beneficial for many individual women who are sexually experienced.     

Serological detection of human papillomavirus type 16 infection in human immunodeficiency virus (HIV)-positive and high-risk HIV-negative women

Serial measurement of antibodies has not been used to provide evidence of active viral replication of human papillomavirus (HPV). Serum specimens from sequential study visits contributed by 642 human immunodeficiency virus (HIV)-positive and 116 HIV-negative participants enrolled in the Women's Interagency HIV Study were used to detect significant rises in HPV type 16 (HPV-16) antibody levels. Factors associated with a significant rise were identified using multivariable logistic regression models with generalized estimating equations. Among HIV-positive women, 8.3% of 1,997 pairs showed antibody rises, compared to 6.1% of 361 pairs among HIV-negative women (P = 0.191). For HIV-positive women, rises were associated with current (odds ratio [OR], 23.4; P < 0.001) or past (OR, 8.9; P < 0.001) HPV-16 infection relative to never being HPV-16 infected and with CD4+ cell counts (OR per 100-cell increase, 0.8; P < 0.001) but not with sexual behavior. For HIV-negative women, rises were associated with past (OR, 10.9; P = 0.033) HPV-16 infection relative to no HPV-16, current cigarette smoking (OR, 5.0; P = 0.029) relative to no smoking history, and having 6 to 10 lifetime sexual partners compared to 0 to 5 partners (OR, 9.9; P = 0.036). Serial measurement of HPV-16 serum antibodies is a useful tool for identifying active HPV-16 viral replication. Rises among HIV-positive women may more often result from reactivation of a latent HPV infection in the context of HIV-induced immunosuppression, while rises among HIV-negative women may more often result from reinfection with HPV.     

太原地区404例女性HPV感染状况及基因分型统计分析

目的探讨太原地区女性人乳头瘤病毒(HPV)感染情况以及基因分型分布及特点。方法采用人乳头瘤病毒基因分型检测试剂盒(PCR技术及导流杂交技术)对太原地区404例女性进行21种HPV亚型分析。结果 404例检测者共检测到HPV阳性患者213例,阳性率为52.7%。21种HPV亚型(除HPV 42、43型外)均有检出,其中检出率较高的高危型有HPV 16(34.4%),HPV 58(13.4%),HPV 53(8.7%);检出率最高的低危型是HPV 6(2.2%)。阳性检出者中单一亚型感染占59.6%,多种亚型感染占40.4%;在多重感染中,伴随着合并感染的亚型数增加,比例逐渐下降。结论利用PCR技术和杂交技术检测女性HPV感染情况,对宫颈癌的早期诊断、预防及疗效观察具有重要的应用价值。     

Human papillomavirus DNA in urine samples of women with or without cervical cancer and their male partners compared with simultaneously collected cervical/penile smear or biopsy specimens.

Infection of specific types of high-risk human papillomaviruses (HPVs) causes cervical cancer in women. Conventional test for genital HPV infection requires collection of scraped cervical cells or biopsy specimens, which involves invasive procedures. Utility of non-invasive urine sampling for detection of HPV in women and their male sexual partners is controversial. The validation of this urine-based HPV DNA test is of immense value not only in screening large population and children but also for HPV vaccine monitoring in adolescents. We examined the frequency of high risk HPV types 16 and 18 in simultaneously collected urine samples and cervical scrapes or biopsy specimens from women with cervical cancer and their single lifetime male sexual partners in order to validate the utility of urine sampling as a reliable non-invasive method for detection of genital HPV infection. Thirty women with invasive cervical cancer and their husbands along with 30 age-matched normal healthy women including their husbands were recruited for the study. Cervical biopsies/scrapes from women subjects and penile scrapes from their husbands and urine samples from all of them were collected before taking biopsy or scrapes. HPV-L1 consensus primer as well as high-risk HPV (HPV 16 and 18) type-specific oligo-primers were used for PCR detection of HPV DNA. The total frequency of HPV in women with cervical cancer was found to be 83% (25/30) while it was only 67% (20/30) in their male partners but there was virtually no difference in results between urine and scrape or tissue biopsy either in women or their male partners. Although healthy women and their husbands showed similar frequency of HPV infection both in urine and scrape samples, there was a significant difference (p=0.05) in the prevalence of high risk HPV type 16 in women with cervical cancer (70%) and their male partners (30%). Similar was the trend between control women and their male partners. The results also showed a very high prevalence of HPV type 16 among Indian women with cervical cancer while its frequency was significantly low in their single lifetime male partners. The case by case matching of HPV positivity and negativity between urine and cervical/penile scrapes or biopsies obtained from women and their male partners demonstrated that the non-invasive urine sampling can be reliably used for screening genital HPV infection in both men and women.     

134例宫颈上皮内瘤变患者HPV分型检测结果分析

目的探讨宫颈上皮内瘤变(CIN)中不同类型人乳头瘤病毒(HPV)的感染及分型情况。方法回顾性研究北京军区总医院妇科门诊134例已经确诊的CIN患者,采用凯普的核酸分子快速导流杂交基因芯片技术对HPV进行分型。结果 1.各级别CIN以HPV高危亚型单一或者多重感染为主;2.在HPV阳性的101例患者中,单一感染者73例(72.28%),双重感染者24例(23.76%),三重感染者3例(2.97%),四重感染者1例(0.99%);3.HPV-16、58感染率最高,分别为30.83%,24.06%。结论 HPV感染以高危型和多重感染为主;随CIN级别的增高,HPV阳性率也是增高的;HPV-16亚型的感染率最高。     

Correlation between human papillomavirus infection and histopathological diagnosis of women in Northeast Brazil

Cervical carcinoma is the fourth leading cause of death among women worldwide. Epidemiological studies claim that human papillomavirus (HPV) infection is a necessary condition for cervical cancer development. Knowledge of the geographic distribution of HPV is important in guiding the introduction of prophylactic vaccines. This study analyzed the prevalence of HPV infection in cervical samples obtained from women with abnormal cervical histopathological diagnosis in Northeast Brazil. The study included an analysis of 211 women whose diagnosis was confirmed for cervical intraepithelial neoplasia type 1 (CIN-1), cervical intraepithelial neoplasia type 2 (CIN-2), cervical intraepithelial neoplasia type 3 (CIN-3), and cancer. The identification of the HPV genotypes was based on the polymerase chain reaction–restriction fragment length polymorphism technique. A total of 42.7% of the samples showed a single HPV infection, while 57.3% showed multiple infections. The most common genotypes detected were HPV-16, HPV-18, and HPV-31. HPV-16, HPV-31, HPV-35, and HPV-18 were the most common types in CIN-1 with a single infection. HPV-16 and HPV-18 were the most often found in CIN-2 with a single infection. HPV-16, HPV-18, and HPV-31 were the most detected in CIN-3 with a single infection. HPV-16 and HPV-31 were the most frequent in cancer with a single infection. Multiple infection with HPV-16 shows a 2.7 times greater risk of CIN-3 (P = .04). Multiple infections for HPV with HPV-16 and excluding the HPV18/31 types, were associated with CIN-3 (P = .01). The results allowed the detection and genotyping of HPV types circulating in the population studied. These findings must be taken into account when devising vaccination strategies against HPV.     

Human papillomavirus genotype spectrum in Czech women: correlation of HPV DNA presence with antibodies against HPV-16, 18, and 33 virus-like particles.

Because the biological spectrum of human papillomavirus (HPV) genotypes present in cervical cancer lesions varies according to the geographical region studied, and because little genotype information is available for Central and Eastern European countries, we studied the endemic HPV-genotype spectrum in cervical samples collected from women visiting gynaecological departments of selected hospitals in the Czech Republic. In a series of 389 samples, 171 (44.0%) were positive for HPV DNA using a consensus-primer polymerase chain reaction (PCR). Genotyping of the HPV PCR products was done using dot-blot hybridisation with type-specific oligonucleotide probes and thermocycle DNA sequencing. Twenty-two different HPV types were detected, HPV-16 being the most prevalent type irrespective of severity of the lesions (55.0%). Multiple HPV types were found in 16.4% of our HPV-DNA-positive samples. The prevalence of HPV infection was 23.0% in women with normal findings and 59.4% in patients with cervical neoplasia, and increased significantly with the severity of the disease: 52.9% in low-grade lesions, 58.0% in high-grade lesions, and 73.5% in cervical carcinomas (P for trend < .00001). In the sera of 191 subjects, 89 with normal findings and 102 with different forms of cervical neoplasia, the prevalence of HPV-specific IgG antibodies was tested by an enzyme-linked immunosorbent assay (ELISA) using virus-like particles (VLPs) of HPV-16, -18, and -33. Antibodies were significantly more prevalent in HPV-DNA-positive than in HPV-DNA-negative women and there was no association with age. In agreement with the results of HPV genotyping, antibodies reactive with HPV-16 VLPs were the most frequent and, moreover, their prevalence increased with the cervical lesion severity. About half of the subjects with smears in which either HPV-16 or HPV-33 DNA had been detected possessed antibodies reactive with homotypic VLPs. With HPV-18-DNA-positive subjects, however, fewer than 25% displayed homotypic antibodies. In general, subjects older than 30 years of age had antibodies reactive to HPV-specific VLPs more often than subjects younger than 30 years of age. In women with benign findings, the seropositivity to HPV-16, -18, and -33 VLPs increased with age, whereas in women with cervical neoplasia the seropositivity decreased with age.