Treatment of Early-stage Adhesive Shoulder Periarthritis with Transcutaneous Electric Stimulation on Acupoints

目的：观察经皮穴位电刺激对粘连前期肩关节周围炎的治疗作用。方法：采用大样本、多中心、随机化等循证医学研究方法，对163例粘连前期肩关节周围炎患者分别进行经皮穴位电刺激治疗和电针治疗，观察经皮穴位电刺激对粘连前期肩周炎的治疗作用及与电针治疗的效应比较。结果与结论：经皮穴位电刺激对粘连前期肩周炎的总有效率达到96．59％，与电针比较无明显差异；该疗法不仅具有明显的止痛效应，对肩关节活动障碍也有明显的改善作用。经皮穴位电刺激为治疗肩周炎有效、简便的疗法。     

Volt-Ampere Characteristics of Acupoint Neiguan （PC 6） on Patients with Hyperthyroidism

Objective： To observe volt-ampere characteristic of Acupoint Neiguan （PC 6） on patients with hyperthyroidism for probing into the actions of acupoints and change of qi and blood of human body as well as the relationship between the action of acupoint and the metabolism of energy. Methods： A self-made high-sensitive apparatus was applied to detect volt-ampere curves of Acupoint Neiguan （PC 6） and its control point on 33 patients with hyperthyroidism and 30 healthy people. Results： In patients, the volt-ampere areas （VAA） of both ampere-increasing scan （AIS） and ampere-decreasing scan （ADS） as well as the inertia areas （IS） of both side Neiguan （PC 6） were significantly smaller than the control points （P〈0.05 or P〈0.01）.     

Clinical Application of Acupoint Neiguan （PC 6）

Acupoint Neiguan （PC 6） is first recorded in Ling Shu （Spritual Pivot）. It is the Luo-Connecting point of the Pericardium Meridian and communicates with acupoint Waiguan （TE 15）; it is also one of eight confluent points, and associates with the Yin Link Vessel. We often use Neiguan （PC 6） alone or as the cardinal acupoint to treat many disorders in clinical practice. It is now reported as follows.     

Effect of Acupuncture at Acupoints Neiguan （PC 6） and Ximen （PC 4） on the Left Cardiac Function in the Patients with Coronary Heart Disease

目的：探讨针刺内关与郄门穴对冠心病患者每博心输出量及左心室排血的峰值流速的影响。方法：60例冠心痛患者分为内关组和郄门组，分别在运针3min后和留针20min后测试每博心输出量，左心室排血的峰值流速。再与针刺前得测试结果相比较。结果：46例患者针刺3min后Q值和VP值较针刺前上升。留针20min后，34例患者Q值和VP值全部上升。结论：针刺内关和郄门可增强左心室壁的收缩能力，使心脏的每博输出量增加，改善冠心病患者的心肌缺血状态。     

Effect of Acupuncture at Acupoint Neiguan （PC 6） on the Myocardial c-fos Gene Expression of Acute Myocardial Ischemia in Rats

目的：探讨电针“内关”改善急性心肌缺血的机制。方法：将96只大鼠随机分为假手术组、心肌缺血模型组、心肌缺血模型加电针组，采用结扎冠状动脉左前降支建立急性心肌缺血实验模型，造模成功后，电针双侧“内关”。用生化方法检测血清心肌酶活性，逆转录聚合酶链反应（RT-PCR）方法检测心肌中c-fos基因的表达。结果：心肌缺血模型组血清心肌酶活性和心肌c-fos mRNA表达显著高于假手术组（P〈0．05），电针后降低，差异有显著性意义（P〈0．05）。结论：电针“内关”改善急性心肌缺血的机制与下调心肌组织c-fos mRNA的表达有关。     

Effects of Hegu （LI 4） and Taichong （LR 3） on Vascular Dilation and Constriction in Migraine Patients

Hegu （LI 4） and Taichong （LR 3） are the Yuan- Primary acupoints of the Large Intestine and Liver Meridian, and are collectively named as Si Guan （four gates） point. Combined use of these two acupoints can harmonize yin and yang, regulate qi and blood, and balance ascending or descending. This paper attempts to study the effects of Si Guan point on the vascular dilation and constriction in migraine patients from the perspective of its feature.     

Effects of Acupuncture at Acupoint Neiguan （PC 6） and Intravenous Injection of Larginine on the Improvement of Acute Myocardial Ischemia

目的：观察和比较针刺“内关”、静脉注射一氧化氯（NO）前体L精氨酸（L-Arg）以及两者联合应用对急性心肌缺血（AMI）大鼠的不同改善效应。方法：采用结扎左冠状动脉前降支建立AMI模型，随机设立对照组、AMI组、LArg治疗组、针刺“内关”治疗组、LArg加针刺“内关”治疗组，观察平均动脉压（MAP）、心率（HR）和心功能多项指标的变化。结果：AMI组以上各项指标较正常对照组明显降低，而各治疗组以上各项指标均有不同程度的改善，其中针药结合治疗组改善最显著。结论：LArg加针刺内关联合应用对AMI有更好的治疗效果。     

Electroacupuncture of Neiguan （PC 6） inhibits enhanced volt- age-gated sodium currents in ischemic ventricular myocytes

OBJECTIVE： To examine the effect of electroacupuncture（EA） at bilateral Neiguan（PC 6） on voltage-gated Na＋currents（INa） and channels（Nav） in ischemic ventricular myocytes.METHODS： EA serum was prepared from six male adult Sprague-Dawley rats that had received EA at bilateral Neiguan（PC 6）. Eighteen ventricular myocytes were prepared from six SD rats using an enzymolysis approach. Myocardial ischemia was mimicked by perfusion of ischemic solution. Whole-cell patch-clamping was used to record three currents evoked from isolated cells. The first current was the control, and recorded in absence of ischemic solution current. The second was the ischemic current,and recorded after perfusion of ischemic solution for 5 min, while the EA current was last, and recorded after perfusion of EA serum for 5 min. Navkinetic curves were fitted using related formulas.RESULTS： Compared with those in controls, in the presence of ischemic solution, peak amplitudes of INasignificantly increased from ﹣40 m V to ＋30 m V,and half-maximal inactivation potentials of Navincreased significantly, while half-maximal activation potentials, slope factors and the recovery time from inactivation to activation of Navwere unchanged. Compared with those in the ischemic solution, in the presence of EA serum, peak ischemic current amplitudes significantly reduced from ﹣40m V to ＋ 40 m V, and half-maximal inactivation potentials were restored, while half-maximal activation potentials, slope factors and the recovery time from inactivation to activation of Navwere unchanged.CONCLUSION： EA at bilateral Neiguan（PC 6） can reduce enhanced INavia restoration of delayed Navinactivation in ischemic ventricular myocytes.     

Influence of Scalp Point-through-point Acupuncture on 200 kDa Neurofilament Protein in Rats with Acute Cerebral Infarction

目的研究头穴透刺对急性脑梗死大鼠神经丝蛋白-200(NF-200)的影响,探讨针刺对脑梗死大鼠神经可塑性影响的机制.方法将健康雄性Wistar大鼠随机分为假手术组(A组)、模型组(B组)、针刺组(C组).通过建立大鼠局灶性脑缺血模型(MCAO),用逆转录-聚合酶链反应(RT-PCR)法,测定以上各组在7 d、14 d、28 d不同时间点NF200 mRNA变化情况.结果头穴透刺组脑组织NF-200的表达与假手术组、造模组相比差异有统计学意义(P＜0.05);而在不同时间窗内头穴透刺组,模型组与假手术组比较差异有统计学意义(P＜0.01).表明头穴透刺可以促进脑组织神经丝蛋白-200的表达.结论头穴透刺能够提高脑缺血后神经功能,促进肢体功能恢复,增加神经丝蛋白-200的表达,发挥对脑组织神经细胞可塑性的调节作用.     

Physical Approaches of Acupuncture,Characterization of the Human Response to Neiguan （PC 6） Puncture by Pulse Signal Analysis

Acupuncture has been recently shown to produce a modification of pulse signals, and more precisely a modification of the power spectrum of these signals. It was shown that not only the main period of the pulse could be changed, but also the harmonicity of the pulse signals was subjected to modification. This result urged the question of a temporal characterization of these signals to complete this frequency analysis, and to search for a transition mechanism at the origin of this modification. The authors propose here a novel approach constructed on a time frequency characterization of pulse signals, based on Morlet wavelet transforms. This allows a temporal survey of the local frequencies （main frequency and its harmonics） of the signal and the measure of their modifications during the acupuncture treatment. The authors not only confirm that the time frequency response may vary during and after the puncture,     

经皮穴位电刺激治疗牙科痛症临床探讨

目的:探讨经皮穴位电刺激在牙科痛症中的作用.方法:采用随机对照法对100例牙科治疗后疼痛患者进行观察,治疗组采用经皮穴位电刺激加西医常规治疗,对照组单纯采用西医常规治疗.结果:治疗组总有效率为96.7%,对照组总有效率为75.0%,两组比较差异有显著性意义(P＜0.05).结论:经皮穴位电刺激法为防止牙科治疗后疼痛的有效方法.     

经皮穴位电刺激在甲状腺肿瘤手术中的应用

目的:探讨经皮穴位电刺激在颈部甲状腺肿瘤手术中减弱应激反应及增强麻醉作用的效果.方法:将60例甲状腺肿瘤手术患者随机分为观察组和对照组各30例,两组病人均采用改良一点法颈丛神经阻滞,观察组加用韩氏穴位神经刺激仪(HANS),选用双侧合谷和曲池穴,术中连续使用HANS至手术结束.结果:观察组血压、心率差、牵拉反应比对照组明显降低,氧饱和度、呼吸频率变化不明显.结论:HANS可以减轻甲状腺肿瘤患者手术时产生的心血管应激反应,同时有强化麻醉的作用.     

电针内麻点和内关穴对胸科手术后镇痛的观察

目的:观察电针内麻点和内关穴对胸部手术后镇痛的有效性及安全性.方法:选取择期行胸部手术患者120例,随机分为电针组(60例)和药物组(60例).电针组使用电针刺激内麻点和内关穴进行手术后镇痛治疗;药物组使用静脉自控镇痛(PCIA)进行手术后镇痛治疗.比较两组患者治疗后视觉模拟量表(VAS)评分、镇痛效果、安全性及血清β-内啡肽含量.结果:两组术后各时点VAS评分与治疗前比较均有降低(均P＜0.0l),且电针组低于药物组(P＜0.01);两组镇痛疗效比较,电针组术后2h优良率为96.7％(58/60),优于药物组的75.0％(45/60,P＜0.01);两组治疗安全性等级比较,电针组高于药物组(P＜0.01);两组患者治疗后血清β-内啡肽含量较治疗前均增高,且电针组高于药物组(P＜0.01).结论:电针刺激内麻点和内关穴对胸科手术后镇痛效果(术后2 h)及安全性均优于PCIA.     

针刺内关穴对心率变异性的分析.

目的：观察了解内关穴对心脏生理的调整效应。方法：采用5分钟心率变异性取样，对24例健康人针刺内关穴前后的心率变异性指标进行对照分析。结果：针刺内关穴时可以使心率波动性增大。心率变异性参数改变。结论：针刺内关穴对健康人的心脏功能起着保护性的调整作用。     

针刺内关穴对窦房结功能的影响

目的:为临床运用内关穴治疗心脏病提供依据。方法:将50例心脏病患者随机分为2组。非阻断组35例,分别于针刺内关穴前后采用食道左房调搏技术测定窦房结恢复时间(SNRT)、窦房传导时间(SACT)、窦房结有效不应期(SNERP)和心率(HR);阻断组15例,用静脉注射心得安和阿托品进行植物神经阻断,分别于阻断前和针刺后测定SNRT、SACT、SNERP和固有心率(IHR)。结果:非阻断组针刺前后比较,SACT、SNERP和HR差异均有统计学意义(均P<0.05),而SNRT差异无统计学意义(P>0.05)。阻断组针刺前后比较,SNRT、SACT和SNERP差异均无统计学意义,而IHR前后差异有统计学意义(P<0.05)。结论:针刺内关穴对窦房结功能有显著作用,其机制可能与针刺内关对窦房结的自主神经具有双相调节作用有关。     

针刺内关穴配合生脉注射液治疗扩张型心肌病所致心律失常疗效观察

目的观察针刺内关穴配合生脉注射液治疗扩张型心肌病所致心律失常的临床疗效。方法将80例扩张型心肌病所致心律失常患者随机分为治疗组和对照组,每组40例。对照组采用常规药物治疗,治疗组在对照组治疗基础上加针刺内关穴配合生脉注射液治疗,观察两组临床疗效。结果治疗1～6个月后,治疗组总有效率为95.0%,对照组为70.0%,两组比较差异具有统计学意义(P<0.05)。结论针刺内关穴配合生脉注射液是一种治疗扩张型心肌病所致心律失常的有效方法。     

艾灸内关穴对冠心病患者心前区皮温的影响

目的探讨心前区皮温与冠心病的发病关系以及艾灸内关穴对冠心病患者心前区皮温的影响。方法对30例健康志愿者(对照组)和30例冠心病患者(治疗组)进行常规胸部A区(心前区)和B区红外线热成像检查,检查后所有受试者给予艾灸内关穴,再对艾灸前后热像图的温度进行对比分析。结果治疗组治疗前A区平均温度与同组B区比较,差异具有统计学意义(P0.05)。治疗组治疗后A区平均温度与同组B区比较,差异具有统计学意义(P0.05)。治疗组治疗后A区平均温度与对照组比较,差异具有统计学意义(P0.05)。结论胸部A区(心前区)皮温在一定程度上反应了冠脉供血状态。艾灸内关穴可以改变胸部A区(心前区)的皮温,具有改善心肌供血的作用。     

电针内关穴为主治疗无症状心肌缺血疗效观察

目的:观察针刺内关穴为主治疗无症状心肌缺血(SMI)的临床疗效.方法:将40例SMI患者随机分为电针组和药物组,每组20例.电针组以手厥阴心包经内关穴为主,结合辨证分型选穴,予电针治疗;药物组口服复方丹参滴丸,比较两组患者总有效率和治疗前后心率、血压、24 h动态心电图的变化情况.结果:电针组总有效率为95.0%(19/20),优于药物组75.0%(15/20,P＜0.05);两组治疗后心率、收缩压和舒张压明显降低(P＜0.01,P＜0.05),电针组较药物组降低明显(均P＜0.05);24 h动态心电图示,两组治疗后SMI段压低持续时间和ST段压低次数均明显减少(P＜0.01,P＜0.05),且电针组优于药物组(P＜0.05).结论:针刺内关穴对SMI具有很好的临床疗效,能减低心率和体循环动脉血压、减轻左室后负荷,优于口服复方丹参滴丸.     

电针内关对内毒素休克大鼠模型肺组织中一氧化氮合酶活性的影响

目的 观察电针内关(PC6)对内毒素休克时肺损伤的保护作用和肺一氧化氮合酶(NOS)表达的影响。方法 SD大鼠尾静脉注射内毒素(LPS)1．5mg／kg、腹腔注射D-氨基半乳糖(D-GaIN)100mg／kg造成内毒素休克模型，用电针PC6和氨基胍(AG)作对照干预处理，用免疫组化方法检测诱生型一氧化氮合酶(iNOS)、内皮型一氧化氮合酶(eNOS)在肺组织内的表达。结果 电针PC6可以使肺内iNOS表达减弱，eNOS表达增强，肺损伤减轻。结论 电针PC6可以减轻内毒素休克造成的肺损伤，这种保护作用可能是通过调节肺组织NOS表达而产生的。