Testing Mycobacterium tuberculosis for drug sensitivity using different methods

Testing 35 Mycobacteria tuberculosis strains isolated from patients for drug sensitivity on the L?wenstein-Yersen, Middlebrook 7 H11 midia and by using the polymerase chain reaction demonstrated a correlations of the results obtained by these methods. In laboratory practice, the use of all possible of guidelines for testing Mycobacteria tuberculosis for drug sensitivity.     

Mycotic pseudoaneurysm caused by Mycobacterium tuberculosis

Mycobacterium tuberculosis can involve any organ of the body, including the arteries. Usually the mycobacteria infects the arterial wall spreading from a contiguous foci. We report a case of tuberculous pseudoaneurysm involving the iliac artery clinically manifested as fever of unknown origin. The diagnosis relied on abdominal CT-scanning. Tuberculous etiology was confirmed postoperatively by microbiologic and microscopic study. The antituberculous therapy was early started, but the patient died three months later as a consequence of a non-infectious abdominal aortic rupture. Clinicians should consider tuberculous etiology when the diagnosis of mycotic pseudoaneurysm is being entertained.     

两种分子药物敏感性试验与表型药物敏感性试验检测结核分枝杆菌耐药性的效能比较

目的：评价基因芯片法和荧光定量PCR探针熔解曲线法直接检测结核病患者临床标本中结核分枝杆菌(MTB)耐药性的价值。方法：选取解放军总医院第八医学中心结核病医学部住院结核病患者临床标本44份(例),以传统药物敏感性试验(简称“药敏试验”)绝对浓度法为对照,应用基因芯片法直接检测临床标本中MTB对利福平、异烟肼的耐药性,以...     

Genitourinary infections caused by mycobacteria other than Mycobacterium tuberculosis

Non-tuberculous mycobacterial infections are usually found in the respiratory tract: infection confined to the genito-urinary system is rare. We report two patients with isolated genito-urinary infections, one due to Mycobacterium chelonae and the other to Mycobacterium avium-intracellulare.     

结核分枝杆菌快速培养药敏方法用于诊断老年矽肺结核

目的评价Bactec-MGIT 960对老年矽肺结核的诊断价值。方法Bactec-MGIT 960对46例老年矽肺结核患者痰标本进行结核杆菌快速培养和药物敏感试验,并与改良7H9法、改良L-J罗氏培养法作比较。结果Bactec-MGIT960、改良7H9法和L-J罗氏培养法培养平均报告时间分别为8.2、24.3、33.6d,培养阳性率分别为69.5%、65.2%、60.8%。Bactec-MGIT 960法药物敏感试验结果与其他检测方法相比,吻合率在90%以上。Bactec-MGIT 960培养、药敏报告时间为24d,较常规方法(J-L罗氏培养法)提前34d出报告。结论Bactec-MGIT 960快速培养结合改良7H9快速药敏试验有助于临床快速诊断。     

肝硬化合并轻微型肝性脑病的临床特点以及相关影响因素分析

[摘要]　目的　观察肝硬化合并轻微型肝性脑病（minimal hepatic encephalopathy, MHE）的临床特点,以及相关临床指标与MHE发生风险的关系。方法?选取87例肝硬化患者,每例患者均进行数字连接试验-A（number connection test-A, NCT-A）测试,测试结果超过正常值上限的患者确定为MHE组,否则为非MHE组。同时收集患者病史及血常规、肝功能、血氨、凝血功能等临床指标,并计算肝功能Child-Pugh评分,通过Logistic回归分析MHE发生的危险因素。结果?87例肝硬化患者中,49例（56.3%）NCT-A异常,诊断为MHE。统计学分析发现MHE组血氨水平明显高于非MHE组（P＜0.05）,但MHE组的血氨水平与NCT-A异常程度无明显相关性。MHE组中,失代偿期肝硬化患者比例、既往发生显性肝性脑病的患者比例、肝功能Child-Pugh评分B&C级患者比例显著高于非MHE组（P均＜0.05）;MHE组年龄偏低,具有更高的TBIL、氨基转移酶水平,而血ALB、CHE、PTA等较非MHE组均显著下降（P均＜0.05）。单因素Logistic回归分析发现,年龄偏低、失代偿期肝硬化、Child-Pugh分级高、血氨水平高、CHE水平低、TBIL水平高、PTA降低均是MHE发生的危险因素（P均 ＜0.05）;多因素Logistic回归分析发现,TBIL水平高是判断MHE发生风险的独立预测因素。结论?肝硬化患者中MHE发生率较高,特别是肝硬化失代偿期患者和肝功能基础较差的患者发生MHE风险显著增加,总胆红素水平高是发生MHE的独立危险因素。     

牛分枝杆菌引起人结核病的概况和控制

牛分枝杆菌是牛结核病的病原体,也可引起人畜共患结核病。虽然由牛分枝杆菌感染引起的人结核病患者例数明显少于由结核分枝杆菌感染引起者,但由于诊断技术的局限性,现有的牛分枝杆菌感染人的流行病学数据并不能准确反映牛分枝杆菌引起的人畜共患结核病的实际流行情况。作者就牛分枝杆菌造成的人畜共患结核病负担、牛分枝杆菌的致病机制、人畜共患结核病对公共卫生的影响,以及控制措施等方面内容进行综述。