RMPP患儿血清IL-2、IL-6、IL-10和TGF-β1检测及分析

目的探讨IL-2、IL-6、IL-10和转化生长因子（TGF）-β1在儿童难治性肺炎支原体肺炎（RMPP）发病中的作用,为临床提供依据。方法选择25例RMPP患儿（RMPP组）,分别于急性期和恢复期采用ELISA法测定血清IL-2、IL-6、IL-10和TGF-β1,并与25例肺炎支原体肺炎（MPP）患儿（MPP组）和15例健康儿童（对照组）对照。结果 RMPP组急性期及恢复期血清IL-2、IL-10水平均显著低于MPP组和对照组,IL-6、TGF-β1水平均显著高于MPP组和对照组（P均〈0.05）;RMPP组和MPP组组内上述各项指标在急性期及恢复期均有显著差异（P均〈0.05）,恢复期MPP组IL-2、IL-6与对照组无显著差异。结论 IL-2、IL-6、IL-10和TGF-β1可能参与了RMPP的发病机制,检测其血清水平可为临床判断MPP病情及预后提供依据。     

结直肠癌患者外周血可溶性IL-2受体和IL-6水平变化与患者生活质量及临床预后的关系

目的探讨结直肠癌患者外周血可溶性白细胞介素-2受体(sIL-2R)和白细胞介素(IL)-6水平变化与患者生活质量和临床预后的关系。方法选择结直肠癌患者86例为结直肠癌组,另选择同期健康体检者47例为对照组,采用酶联免疫吸附法测定两组sIL-2R和IL-6水平,分析sIL-2R和IL-6水平与结直肠癌患者临床资料、术后生活质量及临床预后的关系。结果结直肠癌组外周血sIL-2R和IL-6水平显著高于对照组(P<0.05);外周血sIL-2R和IL-6水平与结直肠癌患者的年龄、性别、肿瘤大小、肿瘤位置、分化程度无关(P>0.05);有淋巴结转移者sIL-2R和IL-6水平显著高于无淋巴结转移者,TNF分期Ⅲ~Ⅳ期者sIL-2R和IL-6水平显著高于Ⅰ~Ⅱ期者(P<0.05);sIL-2R和IL-6高水平组健康状况调查问卷(SF-36)评分、终点事件发生率与sIL-2R和IL-6低水平组比较差异有统计学意义(P<0.05)。结论 sIL-2R和IL-6在结直肠癌患者中呈高水平表达,其表达水平与淋巴结转移情况和临床分期有关,高水平IL-2R和IL-6者术后生活质量较低,临床预后较差。     

阿奇霉素对COPD稳定期患者血清IL-4及IL-10的影响

目的观察阿奇霉素治疗COPD稳定期患者外周血IL-4、IL-10指标的变化。方法选择我院门诊COPD稳定期患者108例,随机分为实验组和对照组(每组54例)。对照组给予常规治疗,实验组在常规治疗上加口服阿奇霉素。两组在治疗前及治疗后3月、6月、9月检测血清IL-4、IL-10水平变化。结果两组患者血清IL-4、IL-10水平在治疗前及治疗后3个月差别无统计学差异(P0.05)。实验组血清IL-4及IL-10在治疗后6月、9月水平均明显高于对照组(P0.05)。结论阿奇霉素具有免疫调节功能,可增加抗炎因子,口服方便、安全、有效,在治疗COPD患者中具有一定临床应用价值。     

食管癌患者血清IL-6、sIL-6R水平的监测及其临床意义

目的研究白细胞介素-6(IL-6)、可溶性IL-6受体(sIL-6R)在食管癌患者血清中的水平变化,以及IL-6与sIL-6R水平在肿瘤临床分期之间的相关关系,并探讨食管癌患者手术前后细胞因子水平的变化。方法用酶联免疫吸附试验(ELISA)方法检测了70例食管癌早期(Ⅰ~Ⅱ)患者、48例食管癌晚期(Ⅲ~Ⅳ)患者和130例对照者血清IL-6、sIL-6R水平,同时对118例食管癌患者手术前后的两项细胞因子指标进行动态观察。结果食管癌早期患者组和食管癌晚期患者组血清IL-6、sIL-6R水平均显著高于正常对照组(P<0.01),晚期患者较早期升高(P<0.01);食管癌转移组手术后血清IL-6、sIL-6R水平比手术前高(P<0.01),无转移组手术后两者水平较手术前明显降低(P<0.01),食管癌患者血清IL-6及sIL-6R水平与肿瘤的恶性化程度有明显相关性。结论sIL-6R在食管癌发病过程中可能起着促进作用,并可以与IL-6起协同作用,两者的免疫失调状态可能与食管癌密切相关;IL-6、sIL-6R水平可作为食管癌病情监测的指标,同时对肿瘤的临床分期、疗效和预后的判断也有一定的帮助。     

血清IFN-γ、IL-4、IL-5及IL-17对儿童哮喘影响及相关性研究

目的探究血清IFN-γ、IL-4、IL-5及IL-17对儿童哮喘影响及相关性。方法分析2013年1月~2014年6月在我院收治的39例哮喘急性期儿童的临床资料,并列为观察组,选取37例在我院进行健康检查的健康儿童作为对照组。结果观察组患者血清IFN-γ、IL-4、IL-5及IL-17水平显著高于对照组,差异具有统计学意义(P0.05);观察组患者VC%、FEV1/FVC%、MMER水平明显低于对照组,差异具有统计学意义(P0.05);观察组患者IFN-γ、IL-4、IL-5、IL-17水平与FEV1/FVC(%)水平呈现显著负相关。结论儿童哮喘发病与Th1、Th2及Th17细胞分泌的IFN-γ、IL-4、IL-5及IL-17有关,其表达水平升高与哮喘的严重程度呈正比。     

支原体肺炎患儿血清炎性细胞因子、免疫球蛋白、补体、hs-CRP水平变化及意义

目的 观察支原体肺炎(MPP)患儿血清炎性细胞因子(IL-2、IL-6)、免疫球蛋白(Ig)和补体、高敏C-反应蛋白(hs-CRP)水平的变化,并探讨其意义.方法 MPP患儿50例(观察组,其中重症28例、轻症22例),健康儿童42例作为对照组.采用ELISA法检测两组血清IL-2、IL-6,用血浆蛋白分析仪速率散射比浊法检测hs-CRP、Ig和补体(C3、C4)水平.结果 与对照组比较,观察组急性期IL-2、IgA水平降低,IL-6、hs-CRP、IgM 、IgG、C3、C4水平升高(P ＜0.05或0.01);恢复期IL-2水平降低,hs-CRP、IgG水平升高(P＜0.05或0.01).与恢复期比较,观察组急性期IL-2水平降低,IL-6、hs-CRP、C3水平升高(P＜0.01).与轻症者比较,重症者IL-2、IgA水平降低,IL-6、hs-CRP、IgM、IgG、C3、C4水平升高(P＜0.05或＜0.01).MPP患儿血清中IL-2与IL-6呈负相关(r=-0.634,P=0.017).结论 MMP急性期、恢复期或重症、轻症患儿血清炎性细胞因子、Ig、补体、hs-CRP水平升高或降低,联合检测上述指标可及时判定MMP病情程度和预后,有助于指导MP患儿的治疗.     

硫酸镁联合雾化吸入布地奈德对急性支气管哮喘患儿血清IL-12、IL-17的影响

目的 探讨硫酸镁联合雾化吸入布地奈德对急性发作支气管哮喘患儿血清白细胞介素-12(IL-12)、白细胞介素-17(IL-17)的影响.方法 86例急性发作支气管哮喘患儿随机分为两组,观察组43例,对照组43例.观察组采用常规治疗加雾化吸入布地奈德和硫酸镁静点滴,对照组采用常规治疗加强的松.治疗7d后测定患儿治疗前后血清的IL-12和IL-17.结果 观察组总有效率为93％,对照组总有效率为83.7％,观察组总有效率高于对照组(P＜0.05).治疗后两组患儿血清IL-12均明显升高,观察组高于对照组(P＜0.05);治疗后两组患儿血清IL-17均降低,观察组低于对照组(P＜0.05).结论 硫酸镁联合雾化吸入布地奈德可以更明显降低血清IL-12和IL-17,进而改善急性发作支气管哮喘患儿病情.     

狼疮性肾炎患者血清IL-6和sIL-2R水平变化的检测

目的探讨狼疮性肾炎(LN)患者血清中白细胞介素(IL)-6和可溶性IL-2受体(sIL-2R)的水平变化及临床意义。方法利用酶联免疫吸附试验(ELISA)检测42例LN患者和40例正常健康人血清IL-6和sIL-2R的水平。结果活动期LN组患者IL-6和sIL-2R水平显著高于静止期LN组患者及正常对照组(P<0.01),静止期LN组患者IL-6和sIL-2R水平显著高于正常对照组,差异有显著性(P<0.01)。结论LN患者血清IL-6和sIL-2R显著升高,可能与LN的发生发展有关,并可作为病情及疗效判断的观察指标。     

老年肺部感染患者IL—6、IL—8和TNF—α测定及其意义

目的探讨老年慢性阻塞性疾病(COPD)患者合并肺部感染时外周血细胞因子白细胞介素6(IL-6)、白细胞介素8(IL-8)、肿瘤坏死因子(TNF-α)和可溶性白细胞介素2受体(sIL-2R)的变化,及其与感染的相互关系.方法采用ABC-HRP方法测IL-6,IL-8.双抗体夹心ABC-ELISA法检测TNF-α,双抗体夹心ELISA检测sIL-2R水平.分别检测了25名正常人(A组)、55例老年COPD合并肺部感染急性期(B组)、21例老年COPD合并肺部感染好转期(C组)患者外周血IL-6、IL-8、TNF-α和sIL-2R的水平.结果B组和C组的血sIL-2R水平均较A组明显升高(P＜0.01).而B组和C组的血sIL-2R水平比较,则无明显差异(P＞0.05).B组的血IL-6水平明显高于A组(P＜0.05).而C组IL-6水平虽高与A组,并且低于B组该值,无统计学上意义(P＞0.05).B组和C组的血IL-8水平均低于A组,但无统计学意义.3组之间TNF-α水平比较,以B组升高最显著,C组次之,分别与对照组比较均有显著差异,P分别＜0.01和0.05.B组与C组比较,也有显著差异(P＜0.05).结论细胞因子IL-6、TNF-α和sIL-2R水平与老年COPD合并肺部感染的严重程度有关,并且3者之间呈正相关.故对其监测,将有利于疾病的分期和活动情况的判断,可作为观察疗效和判断预后的可靠指标.     

白细胞介素-5、可溶性白细胞介素-2受体及一氧化氮与哮喘发病的关系

目的探讨白细胞介素-5(IL-5)、可溶性白细胞介素-2受体(sIL-2R)、一氧化氮(N())及嗜酸性粒细胞(EOS)在哮喘发病中的意义。方法对治疗前后的急性发作期、稳定期哮喘组及对照组血清II-5、sIL-2R和NO水平及EOS计数进行了研究。结果哮喘急性发作组血清IL-5、sIL-2R、NO及EOS水平均明显高于哮喘缓解期组与对照组。哮喘发作期IL-5与EOS呈显著正相关。哮喘急性发作期组治疗后sIL-2R及NO水平较治疗前显著降低，IL-5与EOS水平下降但无统计学意义。结论血清IL-5、sIL-2R、NO及EOS水平升高，与哮喘发作密切相关。     

DOWN-REGULATION OF FIBRINOGEN BIOSYNTHESIS BY IL-4, IL-10 AND IL-13

High levels of fibrinogen are recognized as an important vascular risk factor; however, it is not known if the increase of plasma fibrinogen is directly responsible for this risk, or is only a marker of vascular inflammation. To support this second hypothesis, Oncostatin M (OSM) is a potent stimulator of fibrinogen biosynthesis and induces smooth muscle cell proliferation. In the same way, we analysed whether interleukin-4 (IL-4), interleukin-10 (IL-10) or interleukin-13 (IL-13), which protect vessel walls from monocytes injuries leading to atherosclerosis, could influence fibrinogen biosynthesis. The two levels of regulation of fibrinogen biosynthesis were tested: firstly, the direct effect of these cytokines on fibrinogen production by the hepatoma cell line Hep G2, and secondly their effect on the secretion of hepatocyte stimulating factor (HSF) activity in the supernatant of lipopolysaccharide (LPS)-activated monocytes. IL-4 and IL-13 added to Hep G2 cells down-regulated both the increase of fibrinogen secretion induced by IL-6 and fibrinogen mRNA levels, this effect being more pronounced when Hep G2 were preincubated with the two cytokines before IL-6 addition. The effect of IL-10 was evidenced only on mRNA expression. IL-10 and IL-13 dose-dependently decrease HSF activity secreted by LPS-activated monocytes, whereas IL-4 had no effect. However, the three cytokines decreased HSF activity when monocytes were incubated with the cytokines before LPS activation. The effects of these cytokines on HSF activity are related to variations of IL-6 and OSM secretion. Our data strengthen the hypothesis that the fibrinogen level is a marker of vascular disease, since cytokines which have a protective vascular effect down-regulate fibrinogen production.     

变应性鼻炎和哮喘患者血清IL-9、IL-4、IL-5变化及临床意义

目的探讨血清IL-9、IL-4、IL-5在变应性鼻炎、支气管哮喘（哮喘）、变应性鼻炎合并哮喘中的作用。方法采用双抗体夹心ELISA法检测哮喘组、过敏性鼻炎组、过敏性鼻炎合并哮喘（合并组）患者及查体健康者（对照组）的血清IL-9、IL-4、IL-5。结果与对照组比较,三组患者的血清IL-4、IL-5、IL-9均明显升高（P均〈0．01）;其中血清IL-5合并组高于哮喘组及过敏性鼻炎组（P〈0．01,〈0．05）,过敏性鼻炎组高于哮喘组（P〈0．01）;IL-4合并组高于哮喘组（P〈0．05）。结论IL-4、IL-5、IL-9参与过敏性鼻炎和哮喘的发病,三组患者均表现为Th2型细胞因子过度表达;变应性鼻炎合并哮喘的炎症程度较高,哮喘和鼻炎也有不同炎症反应。     

IL-2I、L-21诱导人外周血单个核细胞及其抗肿瘤作用

目的探讨IL-2I、L-21对人外周血单个核细胞（PBMC）的诱导增殖和表型改变的影响及其体外抗瘤作用。方法取PBMC细胞,调整浓度为1×10^6个/ml,分为四组I,L-2组加入IL-2 100 IU/mlI,L-21组加入IL-21 100IU/ml,联合组加入IL-2 50 IU/ml和IL-21 50 IU/ml,对照组加入0.1 ml生理盐水,台盼蓝染色法计数各组活细胞,流式细胞仪检测各组PBMC表型;以上述四组为效应细胞（E）,以对数生长期的4种肿瘤细胞（人胃癌细胞系M85,胃癌细胞系BGC823,结肠癌细胞系HCT116,结直肠腺癌细胞系HCT8）为靶细胞（T）,稀释靶细胞5×10^3个/孔,E∶T为1∶1和2∶1,MTT法测定细胞毒性（杀伤率）。结果联合组活细胞计数高于IL-2组I、L-21组、对照组,P均〈0.05;联合组I、L-2组和IL-21组CD3^-/CD56^＋、CD3^＋/CD56^＋水平高于对照组,P〈0.05或0.01;联合组对4种肿瘤细胞的杀伤率均高于其余三组I,L-2组I、L-21组高于对照组,P均〈0.05。结论 IL-2联合IL-21的协同刺激作用,可有效地刺激PBMC的增殖和表型改变,对4种消化道肿瘤细胞株均有较强的杀伤力。     

Serum interleukin-6 (IL-6) and interleukin-4 (IL-4) in patients with multiple myeloma (MM)

In this study we determined, in patients with multiple myeloma (MM), serum levels of IL-4 and IL-6 at diagnosis and during the course of the disease, seeking a correlation with disease activity and prognosis. We studied 54 MM patients, 41 of whom responded to chemotherapy whilst 11 were resistant. At diagnosis, IL-6 was increased in 66% of patients (median 35.5 pg/ml) whereas IL-4 was low (median 4 pg/ml) in 75% of patients. In responding patients, IL-4 increased in remission (median 25 pg/ml), whereas IL-6 decreased (median 4 pg/ml). In chemotherapy-resistant patients, IL-6 and IL-4 values remained stable during the course of the disease.     

白细胞介素-1β和白细胞介素-6对妊娠相关血浆蛋白-A表达的影响

目的探讨炎性因子白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-6(interleukin-6,IL-6)对人冠状动脉平滑肌细胞(human coronary artery smooth muscle cell,HCASMC)表达妊娠相关血浆蛋白-A(pregnancy-associated plasma pro-tein-A,PAPP-A)的影响。方法应用20μg/L的IL-1β、10μg/L的IL-6各自刺激HCASMC,共同培养0、2、4、8、243、6 h后收集细胞。应用不同浓度的IL-1β(0、5、20、40μg/L)I、L-6(0、5、10、50μg/L)刺激HCASMC,共同培养6 h后收集细胞。应用实时定量聚合酶链反应的方法检测细胞内PAPP-A基因的表达量。结果在同剂量IL-1βI、L-6刺激下,PAPP-A的表达量在2 h时就开始发生上调,8 h达高峰,而后开始下降;在不同剂量IL-1βI、L-6刺激下,PAPP-A的表达量在实验剂量范围内随着剂量的加大呈上升趋势(IL-1β:r=0.972,P=0.000;IL-6:r=0.941,P=0.000)。结论炎性因子IL-1βI、L-6能促进HCASMC中斑块稳定相关标记物PAPP-A的表达,可能是炎症在急性冠状动脉综合征发生发展中的重要作用机制之一。     

Interleukin (IL)-17 promotes macrophages to produce IL-8, IL-6 and tumour necrosis factor-alpha in aplastic anaemia

Aplastic anaemia (AA) is thought to be an autoimmune-mediated disease with active destruction of haematopoietic cells through a T helper type 1 (Th1) cell response. Interleukin (IL)-17 is a potent proinflammatory cytokine produced by activated memory T cells. Recent studies indicate that IL-17 might be an essential effector cytokine in the T-cell mediated autoimmune process. It can drive the production of tumour necrosis factor-α (TNF-α), IL-1 β, IL-6 and IL-8 by a variety of cells. The present study investigated the genetic and protein expression of IL-17 in patients with AA. The effect of IL-17 on IL-6 and IL-8 production by macrophages was also studied. AA patients showed an elevated expression of IL17A mRNA in bone marrow mononuclear cells and peripheral blood mononuclear cells. Higher IL-17 in bone marrow and peripheral blood plasma was also observed in AA patients compared with normal controls. IL-17 induced the production of IL-6 and IL-8 by macrophages both from patients with AA and normal controls. IL-17 stimulation also resulted in the production of TNF-α. These results suggested that elevated expression of IL-17 and IL-17-induced IL-6, IL-8 and TNF-α may be involved in the mechanisms of AA.     

Prognostic value of serum IL-10 and soluble IL-2 receptor levels in aggressive non-Hodgkin's lymphoma

Summary We investigated the prognostic significance of interleukin-10 (IL-10) and soluble interleuckin-2 receptor (sIl-2r) levles in the pretreatment serum of 105 individuals with newly-diagnosed aggressive non-Hodgkin's lymphoma (NHL). Commercially available enzyem-linked immunoassay kits were used for cytokine and receptor measurements. Detectable levels of IL-10 were found in 42 (40%) patients at diagnosis, with no correlation with clinico-haematological parameters, but in no control samples (P < 0.001).
Pretreatment concentrations of sIL-2r were markedly increased in individuals with NHL when compared to controls (2614 ± 893 U/ml v 219 ± 65U/ml, P < 0.001), patients with stage III/IV presenting higher values than those with stage II disase (3885 ± 1196U/ml v 1732 ± 646U/ml, P < 0.001). No single parameter was associated with the achiveement of complete remission, but the combination of elevated IL-10 and of sIL-2r greater than 3000U/ml selected a subset of patients with a high probability of failing induction therapy (P < 0.001). Lifetable analysis also indicated thatj patients with these characteristics have a significantly shorter event-free survival. In a multivariate analysis the combination of IL-10 with sIL-2r was found to have greater predictive strength than the combination of IL-10 with β₂-micro-globulin. We conclude that IL-10 and sIL-2r measurements can be expected to improve existing methods of risk assignment in aggressive NHL.     

乌梅丸对溃疡性结肠炎大鼠结肠组织白细胞介素8及13含量的影响

[目的]观察乌梅丸对溃疡性结肠炎大鼠结肠组织白细胞介素(IL)-8、IL-13的影响.[方法]84只SD大鼠随机分为正常对照组,模型组,柳氮磺胺吡啶( SASP)组,乌梅丸大、中、小剂量组,每组14只;除正常组外,其他各组大鼠采用2.4-二硝基氯苯(DNCB)加醋酸复合法制备溃疡性结肠炎模型;采用双抗体夹心ELISA法检测各组大鼠结肠组织IL-8、IL-13的含量.[结果]模型组大鼠结肠组织中IL-8含量较正常组显著增高(P＜0.01),乌梅丸各剂量组、SASP组则较模型组明显降低(P＜0.01或P＜0.05);模型组大鼠结肠组织IL-13含量较正常组显著降低(P＜0.01),乌梅丸各剂量组、SASP组较模型组明显升高(P＜0.01或P＜0.05).乌梅丸大剂量组降低IL-8、升高IL-13的效果最显著,其IL-8、IL-13的含量与正常组比较差异无统计学意义.[结论]IL-8、IL-13参与了UC的发生、发展,乌梅丸能通过降低溃疡性结肠炎大鼠结肠组织IL-8的含量、升高IL-13的含量,从而起到治疗UC的作用.     

Dexamethasone plus retinoids decrease IL-6/IL-6 receptor and induce apoptosis in myeloma cells

Interleukin 6 (IL-6) is the most important known growth factor for multiple myeloma, and IL-6 signalling pathways are potential targets for therapy. We hypothesized that interfering with the IL-6 signalling pathway at more than one level would be more effective than a single block in inhibiting proliferation of myeloma cells. Accumulating data support the concept that glucocorticoids down-regulate IL-6, whereas retinoic acid derivatives (RA) down-regulate IL-6R in myeloma. We found that all- trans RA (ATRA), 13- cis -RA and 9- cis -RA each similarly inhibited growth of RPMI 8226 myeloma cells and that addition of dexamethasone (DEX) added to RA growth inhibition. The major effects of retinoids were to reduce the proliferative fraction and induce apoptosis whereas DEX increased the apoptotic fraction. When combined, apoptosis was enhanced. Effects of RA + DEX were also least able to be overcome by exogenous IL-6. RA decreased IL-6R levels and addition of DEX to RA delayed recovery of IL-6R levels compared with RA alone. Since RPMI 8226 cells have undetectable IL-6, we investigated U266B1 cells and found that RA and DEX decreased both IL-6 secretion and IL-6 RNA levels. Mechanistically, IL-6R down-regulation by RA was enhanced by DEX, whereas IL-6 protein and RNA levels were reduced by DEX and by RA. In summary, combinations of RA + DEX were not only more effective in inhibiting myeloma cells growth by the dual mechanisms of decreasing proliferative fraction and increasing apoptotic fraction, but were also less able to be overcome by IL-6.