miR-630对H2O2诱导的人晶状体上皮细胞的增殖、凋亡与氧化损伤的影响

目的 探讨miR-630对H₂O₂诱导的人晶状体上皮细胞的增殖、凋亡与氧化损伤的影响。方法 将HLE-B3细胞分为对照组、H₂O₂组、H₂O₂₊阴性对照组(H₂O_2+miR-NC)和H₂O_2+miR-630抑制剂组(H₂O_2+miR-630 inhibitor)。qRT-PCR检测各组细胞中miR-630的表达水平。细胞集落形成实验和EdU实验分析各组细胞的增殖能力。细胞划痕愈合实验分析各组细胞的迁移能力。Annexin Ⅴ-FITC/PI分析各组细胞凋亡能力。试剂盒检测ROS水平和SOD含量。Western blot法检测各组细胞中Bcl-2和Akt蛋白的表达。结果 与对照组比较,H₂O₂组和H₂O_2+miR-NC组中miR-630的表达显著上调(P=0.000);与H₂O₂组和H₂O_2+miR-NC组比较,低表达miR-630使HLE-B3细胞中miR-630的表达降低。与对照组比较,H₂O₂组和H₂O_2+miR-NC组HLE-B3的增殖能力(P均<0.000)、迁移能力显著下降(P均=0.000),凋亡能力明显提高(P均=0.000);与H₂O₂组和H₂O_2+miR-NC组比较,H₂O_2+miR-630 inhibitor组HLE-B3细胞的增殖能力(P均<0.01)、迁移能力提高(P均<0.05),凋亡能力显著下降(P=0.000)。与对照组比较,H₂O₂组和H₂O_2+miR-NC组中ROS水平上调(P均=0.000)、SOD含量减少(P=0.000)、Bcl-2和Akt蛋白表达水平下调(P均=0.000);与H₂O₂组和H₂O_2+miR-NC组比较,H₂O_2+miR-630 inhibitor组中ROS水平下调(P均<0.01)、SOD含量增加(P<0.05)、Bcl-2和Akt蛋白表达水平上调(P<0.01,P<0.05)。结论 低表达miR-630可能促进H₂O₂诱导的人晶状体上皮细胞的细胞增殖、迁移,抑制细胞凋亡和减少氧化损伤,其可能与Bcl-2和Akt的表达上调相关。     

支气管镜介入治疗结合全身联合用药在儿童难治性支原体肺炎的疗效分析

目的 探讨支气管镜结合全身联合用药治疗儿童难治性肺炎支原体肺炎(RMPP)的临床疗效。方法 回顾性分析我院2018年3月至2020年3月的RMPP患儿临床资料,共205例,分为两组,其中气管镜组114例与对照组91例(未行支气管镜)。比较两组患儿一般资料、影像学检查、发热天数、住院天数、肺外并发症、实验室检查结果及全身联合用药使用情况。结果 ①气管镜检查治疗后患儿咳嗽发热等临床症状明显改善,住院天数(t= -2.514,P=0.013)及退热天数(t= -4.774,P=0.000)较对照组缩短。② 两组患者均常规使用大环内酯类抗生素,气管镜组较对照组联合糖皮质激素及丙种球蛋白使用率较对照组低[46(40.4)vs58(63.7)](X_²=11.072 _^a ,P=0.001)。与对照组比较,气管镜组联合使用糖皮质激素减少(X_²=10.454_^a,P=0.015)、联合使用免疫球蛋白减少(X_²=10.395_^a,P=0.001)。 ③ 治疗后复查生化指标,气管镜组较对照组CRP、LDH 、IgE检查指标均下降,CRP(t= -1.901,P=0.036)、LDH(t= -0.473,P=0.025)、IgE(t= -1.400,P=0.042)。结论 支气管镜对于治疗难治性支原体肺炎治疗起关键作用,支气管镜联合糖皮质激素及丙种球蛋白治疗比以往单一依靠药物治疗疗效肯定,能够促进疾病康复。     

气囊面罩联合插管在急诊心肺复苏中的效果观察

目的：探讨气囊面罩通气联合气管插管治疗急诊心肺复苏的临床疗效。方法选取80例呼吸心跳骤停后行心肺复苏的患者,随机将其分为2组：对照组40例,给予普通气囊面罩通气治疗;观察组40例,给予气囊面罩通气联合气管插管治疗。观察比较2组患者的脉搏血氧饱和度(SpO 2)、平均动脉血压(MAP)、复苏成功率及存活率。结果2组患者抢救结束后,2组SpO 2和MAP比较,差异有统计学意义(t=18.572,P=0.000;t=18.832, P=0.000);2组心肺复苏抢救成功率分别为12.5%和47.5%,差异有统计学意义(χ2=5.833,P=0.000）;最终存活率分别为5.0%和32.5%,差异有统计学意义(χ2=4.964,P=0.000)。结论在急诊心肺复苏时,及时有效地开放气道是抢救的关键,使用气囊面罩联合气管插管能够显著提高抢救成功率和存活率。     

同步间歇指令通气模式下肺内、外源性急性呼吸窘迫综合征通气治疗的效果比较

目的 探讨同步间歇指令通气模式下肺内、外源性急性呼吸窘迫综合征（ARDS）患者侧卧位与俯卧位治疗效果。方法 选取2020年6月—2021年12月宣城市人民医院收治的ARDS患者120例，根据病因及通气方法分为4组，其中肺内源性ARDS侧卧位治疗患者作为A组（29例）、肺外源性ARDS患者侧卧位治疗患者作为B组（30例）、肺内源性ARDS俯卧位治疗患者作为C组（31例）、肺外源性ARDS俯卧位治疗患者作为D组（30例）。对比4组患者治疗前后血气指标、呼吸力学指标、心功能指标、血流动力学指标的变化，记录患者治疗期间并发症、机械通气时间及近期预后。结果 4组患者体位变化前、体位变化1 h、体位变化3 h的PaO₂、PaO₂/FiO₂比较，结果 ①不同时间点PaO₂、PaO₂/FiO₂有差异（F =8.365和9.001，均P =0.000）；②4组患者PaO₂、PaO₂/FiO₂有差异（F =9.025和8.747，均P =0.000）；③4组患者PaO₂、PaO₂/FiO₂变化趋势有差异（F =8.779和7.988，均P =0.000）。4组患者体位变化前、体位变化1 h、体位变化3 h的气道峰压、呼吸浅快指数、HR、MAP、CVP比较，结果 ①不同时间点气道峰压、呼吸浅快指数、HR、MAP、CVP无差异（F =2.143、1.372、1.854、1.879和1.266，P =0.092、0.252、0.086、0.134和0.287）；②4组患者气道峰压、呼吸浅快指数、HR、MAP、CVP无差异（F =1.869、1.752、1.003、0.803和0.708，P =0.084、0.157、0.459、0.494和0.548）；③4组患者气道峰压、呼吸浅快指数、HR、MAP、CVP变化趋势无差异（F =2.246、1.657、1.963、2.010和2.225，P =0.079、0.177、0.080、0.114和0.086）。4组患者体位变化前、体位变化1 h、体位变化3 h的LVEF比较，结果 ①不同时间点LVEF有差异（F =6.011，P =0.000）；②4组患者LVEF无差异（F =1.957，P =0.081）；③4组患者LVEF变化趋势有差异（F =6.032，P =0.000）。4组患者治疗期间均无明显并发症发生。4组患者机械通气时间、病死率比较，差异均无统计学意义（P >0.05）。结论 同步间歇指令通气模式下肺外源性ARDS患者体位变化通气治疗血气指标改善优于肺内源性ARDS患者，俯卧位通气治疗ARDS对肺通气改善优于侧卧位。     

中医外治法治疗风热客睑型麦粒肿的临床观察

目的：探讨中医外治法治疗风热客睑型麦粒肿的临床疗效。方法：纳入就诊于临平区中西医结合医院眼科门诊的风热客睑型麦粒肿患者共126例(126只眼),分为2组,其中对照组62例(62只眼)予常规治疗(左氧氟沙星滴眼液 4次/日 滴患眼,眼睑局部进行热敷),治疗组64例(64只眼)在对照组的基础上联合中医外治法(耳尖放血、中药熏蒸)。观察两组患者眼表征象改善情况、肿块消退情况及治疗1d、3d、5d后的治愈率。结果：(1)眼表征象改善情况：治疗后治疗组红肿、疼痛、硬结情况较对照组均有明显改善(c_2^红肿=8.50,P_红肿=0.004；c_2^疼痛=4.33,P_疼痛=0.038；c_2^硬结=2.82,P_硬结=0.093)。(2)肿块消退情况：治疗后肿块最大直径治疗组(1.10±0.86)mm,对照组(1.60±1.33)mm,两组间比较有统计学意义(t=-2.52,P=0.013)。治疗前后肿块最大直径差值治疗组(4.58±1.31)mm,对照组(3.93±1.27)mm,两组间比较有统计学意义(t=-2.80,P＜0.001)。(3)治愈率：治疗1d、3d、5d后治疗组的治愈率分别为20.3%、51.6%及85.9%,均高于对照组(c_2^1d=5.18,P_1d=0.023；c_2^3d=7.67,P_3d=0.006；c_2^5d=8.82,P_5d=0.003)。结论：中医外治法联合常规治疗在改善风热客睑型麦粒肿眼部征象、提高治愈率方面优于常规治疗。     

P16和P15在恶性黑色素瘤中的表达及其临床意义

目的 探讨P16和P15在恶性黑色素瘤中的表达及其临床意义。方法 采用SP法免疫组化检测66例恶性黑色素瘤组织中P16、P15的表达情况,统计患者的临床资料分析其表达与临床特征的相关性,采用Kaplan-Meier曲线对随访数据进行预后分析。结果 恶性黑色素瘤中P16(χ²=33.470, P=0.000)、P15(χ²=51.603, P=0.000)的表达率均显著低于皮内痣组织。恶性黑色素瘤中P16的阳性表达水平与肿瘤浸润深度(χ²=17.888, P=0.000)、淋巴结转移(χ²=5.561, P=0.018)和Ki-67增殖指数(χ²=7.108, P=0.008)呈负相关;P15的阳性表达水平与肿瘤浸润深度(χ²=9.156, P=0.000)、淋巴结转移(χ²=8.817, P=0.003)、远处转移(χ²=4.970, P=0.026)和Ki-67增殖指数(χ²=15.422, P=0.000)呈负相关。P16、P15表达阴性的患者,中位总体生存期明显短于表达阳性的患者(14.0个月 vs 28.6个月,P=0.025;14.0个月 vs 33.4个月, P=0.003)。结论 P16和P15可能在恶性黑色素瘤的发生和发展中起负性作用,可以作为鉴别恶性黑色素瘤和良性黑素细胞病变的指标,也可能对MM患者的预后有一定的指导意义。     

高强化在甲状腺CT双期增强扫描良、恶性结节性质判断中的价值

目的 探讨高强化在甲状腺CT双期增强扫描良、恶性结节性质判断中的价值。方法 回顾分析经手术及病理证实的686例1019枚甲状腺结节的CT平扫和双期增强扫描(注药后25s和50s)资料,其中良性结节701枚,恶性结节318枚,分别统计25s、50s、25s 50s和25s或50s扫描序列中,高强化在良、恶性结节中的分布,计算四组扫描序列中高强化对良性结节诊断的敏感性和特异性。结果 25s、50s、25s 50s和25s或50s四组扫描序列中,高强化在良、恶性结节中发生率分别为9.3%(65/701)和1.9%(6/318)(χ₂=18.409,P=0.000)、10.4%(73/701)和1.6%(5/318)(χ₂=24.192,P=0.000)、7.6%(53/701)和1.3%(4/318)(χ₂=16.456,P=0.000)、12.1%(85/701)和2.2%(7/318)(χ₂=26.233,P=0.000),高强化对良性结节诊断的敏感性和特异性在各组中分别为9.3%和98.1%、10.4%和98.4%、7.6%和98.7%、12.1%和97.8%。结论 在四组扫描序列中,尽管敏感性均存在一定不足,但高度的特异性提示双期增强扫描中的高强化有助于良性结节的诊断,其中单期增强50s优于25s扫描,25s或50s在保持高度特异性的基础上具有最高的敏感性,为减少不必要的手术创伤提供了重要的依据。     

重度COPD患者营养风险与运动耐量的相关性研究

目的 探讨重度慢性阻塞性肺疾病(chronic obstructive pulmonary disease, COPD)患者营养风险与运动耐量的关系。 方法 根据营养风险筛查2002标准(NRS-2002)对58例重度COPD患者进行营养风险评估,分为无营养风险组(A组,n=33)及有营养风险组(B组,n=25)。两组分别进行血气分析(arterial blood gas, ABG)、常规肺功能(pulmonary function test, PFT)及心肺运动试验(cardiopulmonary exercise test, CPET)。 结果 两组间的年龄和身高差异无统计学意义(P＞0.05)。B组体质量及BMI低于A组(P＜0.01)。血气分析中两组间的p_aO₂和p_aCO₂差异无统计学意义(P＞0.05)。肺功能检测B组DLCO显著低于A组(P＜0.05)。而两组间其他指标(FEV₁、FEV₁%pred、FVC、FEV₁/FVC、RV及RV/TLC)差异无统计学意义(P均＞0.05)。心肺运动试验检查B组Peak V· O₂、Peak V· O₂%pred及Peak O₂ pulse显著低于A组(P＜0.05, P＜0.01)。两组间Peak V· O₂/kg及Peak Load差异无统计学意义(P＞0.05)。58例重度COPD患者的NRS评分与Peak V· O₂呈显著负相关(r=-0.353,P＜0.01)。58例重度COPD患者的NRS评分与Peak O₂ pulse、Peak Load呈负相关(r=-0.322、-0.272,P均＜0.05)。 结论 有营养风险的重度COPD患者的肺换气功能减低更显著,运动耐量下降更明显。重度COPD患者的NRS评分可预测其运动耐量。     

PBL教学法结合情景教学在心肺复苏教学的应用

目的 探讨PBL教学法结合情景教学对医学生心肺复苏教学质量的影响。方法 选取2020年6月1日-2020年12月31日兰州某大学医学院本科实习生100名,随机分为观察组和对照组,每组各50人。观察组采用PBL教学法结合情景教学进行心肺复苏教学,对照组采用传统教学法进行心肺复苏教学。课程结束后,比较2组学生心肺复苏理论考试和心肺复苏操作质量,采用问卷调查评估观察组学生对PBL教学法结合情景教学的认可度。结果 观察组和对照组学生心肺复苏的理论和操作考核成绩分别为(87.8±4.2vs 82.0±3.5)分和(83.7±3.0 vs 75.1±3.8)分,有统计学差异(t=4.790 P=0.001;t=8.013 P=0.007));与对照组相比,观察组学生心肺复苏的胸外按压质量明显高,有统计学差异(P<0.05);观察组中90%学生在问卷调查中认可PBL教学法结合情景教学。结论 在医学生心肺复苏教学过程中,应用PBL教学法结合情景教学效果优于传统教学法,能够培养学生的各项临床实践能力,提高心肺复苏质量,值得推广。     

《2010AHA指南》对上海市院前心肺复苏成功率的影响

目的:分析我中心在执行《2010美国心脏协会心肺复苏及心血管急救指南》(下称《2010AHA指南》)前后4年的院前心肺复苏成功病历,探讨《2010AHA指南》中对心肺复苏CPR流程及技术参数的调整对院前心肺复苏成功率的影响。方法:对照我中心执行《2010AHA指南》前后各2年的复苏成功病例524例进行数据统计和分析。结果:①两组发病年龄具有相同的分布特点,对复苏成功无显著影响。②执行CABD流程后胸外按压开始时间缩短I组为(4.82±2.55)秒,明显高于Ⅱ组(1.91±1.43)秒,两组比较差异有统计学意义(t=15.05,P=0.0000.05)。③院前急救心脏骤停患者复苏成功率(I组为2.35%,Ⅱ组为3.24%P0.050)有统计学差异。④复苏成功患者中存活出院率(I组为5.21%,Ⅱ组为11.50%P0.050)有统计学差异。⑤有目击者第一时间实施胸外按压,复苏成功率17.1%,无目击者第一时间实施胸外按压,复苏成功率1.22%。两组复苏成功率差异有统计学意义(χ2=1544.33,P=0.0000.05)。结论:CPR中采取CABD复苏流程更为合理和有效。尽早而有效的胸外按压在早期复苏中可维持一定动脉压,提供重要脏器对氧的基本需求,有利于心肺复苏成,并认为公众自救早期实施胸外按压有利于提高心肺复苏成功率。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.