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1.
目的:抗ENA抗体谱的临床意义与其他免疫指标的比较,方法:对103例自身免疫患者血清标本,采用免疫印迹法检测抗体,经免疫散射比浊法检测免疫球蛋白和补体,结果:抗ENA抗体在自身免疫病有较高的检出率,与正常对照组比较,抗ENA抗体阳性血清中IgG、IgM、IgA增设,而C3和总补体溶血性(CH5O)水平下降,随着抗ENA抗体免疫印迹条带数的增多,IgG增设,而IgM、C3及CH5O有明显的下降,C3、CH5O与疾病活动性有关系。结论:抗ENA抗体阳性者存在体液免疫应答增加,并且与抗ENA抗体免疫印迹条带数呈正比。 相似文献
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目的探讨三种方法对TORCH—IgM、IgG抗体检测结果的相关性,评价免疫印迹法在临床上检测TORCH—IgM、IgG抗体的应用价值。方法应用酶联免疫法(ELISA)、微粒子酶免疫法(MEIA)和免疫印迹法(WB)对196份临床孕妇筛查样本进行TORCH—IgM、IgG抗体检测,分析各种方法的抗体检出率和总符合率。结果ELISA、MEIA和wB检测TORCH—IgG抗体符合率分别为抗HSVl/2IgG为90%,抗CMVIgG为91%,抗RvIgG为84%,抗ToxIgG为98%。检测TORCH—IgM抗体符合率分别为抗HSV1/2IgM为87%,抗CMVIgM为89%,抗RvIgM为85%,抗ToxIgM为94%。结论WB检测临床标本TORCH—IgM、IgG抗体结果与ELISA、MEIA法所获得的结果具有较高的符合率,其灵敏度高、特异强为临床检测TORCH—IgM、IgG抗体提供了新的检测手段。 相似文献
3.
用活检粘膜细胞培养、水素酶试验和病理切片HE染色等方法对60例有症状儿童了幽门螺杆菌(HP)感染状况的检测,在此基础上建立了检测抗HpIgG抗体的免疫印这方法。引入牛定量积分法表示结果,IgG积分≥2是性,则免疫印迹方法检测抗HpIgG抗休的敏感性为91.1%,特异性为80.0%。用免疫印这技术对儿童血清中HpIgG、IgA、IgM抗体进行分析前与成人相比较,结果发现:与成人相比,儿童IgG识别条 相似文献
4.
目的:探讨儿童再发性腹痛与幽门螺杆菌感染的关系。方法;对66例经排除了肠道蛔虫症、肝、胆、胰及腹外科疾病的非精神心理因素所致的再发性腹痛患儿进行胃镜及幽门螺杆菌(HP)检测。采用三种方法检测HP:免疫印迹法、^13C-UBT及组织学染色找菌。所有病例均进行胃窦粘膜活检检查。结果:胃镜所见:政党例(7.58%),慢性胃炎61例(92.42%),慢性胃炎合并十二指肠球炎9例(13.64%),合并食管炎1例(1.52%),合并胆汁返流5例(7.58%),合并胃粘膜脱垂1例(1.52%),合并消化性溃疡8例(12.12%),HP阳性率33.33%。66例均进行了病理学检查,观察到胃炎症状与HP感染相关,66例中胃窦粘膜活检正常3例,Giemsa染色找菌HP全部阴性,慢性浅表性胃炎轻度37例,Giemsa染色HP阳性6例,慢性浅表性胃炎中-重度26例,Giemsa染色阳性16例。结论:儿童再发性腹痛多数存在器质性病变,并与HP感染有关,对经排除肠道蛔虫症、肝、胆、胰及腹外科疾病的非精神心理因素所致的再发性腹痛患儿,尤其血清HP-IgG抗体或(及)^13C-BUT检测阳性者,为进一步行胃镜检查以明确诊断。 相似文献
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目的为了解肠道病毒71型(EV71)和柯萨奇病毒A16型(CoxA16)在深圳市健康人群中的隐性感染情况,进一步探讨隐性感染在儿童手足口病(HFMD)中的传播特征及危害性。方法实验采用酶联免疫(ELISA)法检测不同年龄层(0~59岁之间)的健康人群血清中抗肠道病毒71型(EV71)和柯萨奇病毒A16型(CoxA16)的IgM和IgG抗体水平,并采用荧光定量PCR检测IgM阳性的样本中病毒核酸的阳性率。结果抗EV71和CoxA16 IgG抗体的阳性率都随着年龄段的增长呈下降趋势,0~5岁儿童组的IgG抗体阳性率最高,其中抗EV71的IgG抗体阳性率为79.7%,抗CoxA16的IgG抗体阳性率为91.93%。30岁以下者,两种病毒的抗体阳性率在70%以上,显著高于30岁以上人群,P〈0.01。5岁以下儿童抗EV71抗IgM抗体阳性率达15.9%。49份抗EV71 IgM阳性样本中有42份EV71的核酸阳性,阳性率为85.7%;31份抗CoxA16 IgM阳性样本中有25份CoxA16核酸阳性样本,阳性率为80.6%。结论人群对EV71和CoxA16普遍容易感染,健康人群中存在较高的隐性感染,隐性感染率与人群的年龄分布存在相关性,因此隐性感染人群也是病原体传播的传染源之一。 相似文献
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目的 探讨幽门螺杆菌(Hp)感染与老年人心脑疾病的关系。方法 采用酶联免疫法检测82例老年人心脑疾病患者和42例健康人血清Hp抗体IgG、IgA、IgM的含量,并进行对照分析。结果 心肌梗塞组与脑梗塞组的Hp抗体IgG、IgA、IgM阳性率均分别达到80%、60%、20%以上,两组结果比较差异无显著性(P〉0.05)。对照组Hp抗体IgG、IgA、IgM阳性率分别为44.6%、25.5%、12.8%,明显低于心肌梗塞组和脑梗塞组。结论 Hp感染可能与老年人心脑疾病的发病有关。 相似文献
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武悦 《中华综合医学杂志(哈尔滨)》2005,6(2):109-110
目的:对呼吸道感染患者进行肺炎支原体(Mp)检测,以期协助临床早诊断、早治疗。方法:对980例呼吸遥感染者同时作冷凝集试验并用间接免疫荧光法检测抗-Mp-特异性抗体(IgG,IgM)。结果:980例呼吸道感染者,检出Mp特异性抗体阳性180例,阳性率18.4%。〈3岁、-6岁、-14岁、〉14岁4个年龄组Mp特异性抗体阳性率分别为8.1%(12/148)、23.6%(44/186)、31.0%(31/100)、5.3%(3/56)。在186例临床诊断为Mp感染的病例中,抗-Mp—IgG阳性118例,敏感性63、4%,抗-Mp—IgM阳性160例,敏感性86.0%,冷凝集试验阳性86例,敏感性46.2%,抗-Mp—IgM敏感性较冷凝集试验高(P〈0.05)。结论:间接免疫荧光法检测血清Mp抗体可作为早期诊断Mp感染的指标之一。 相似文献
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目的:研究小儿包虫病的免疫诊断方法,探索其抗体应答假阴性反应原因。方法:采用间接ELISA和单克隆双抗体夹心ELISA方法,对55例小儿包虫病患者血清的IgG亚类(IgGl、IgG2、IgG3和IgG4)、IgA、IgM、IgE类抗体、抗原和循环免疫复合物进行检测。结果:8种抗体检查方法中IgGl亚型抗体的敏感性和特异性最好,IgGl分别与其它种抗体的1种或2种联合检测,以IgGl IgA IgM检出率最高,为64.3%;15例IgG抗体阴性小儿患者中,有12例分别检测出IgG亚类和/或IgM、IgA、IgE,3例病人血清各种抗体均呈阴性反应,其中IgGl、IgG4及IgA、IgM、IgE的特异性抗体检出率明显高于正常人,分别为42.9%、ll.9%、28.6%、26.2%和21.6%;小儿的IgM水平高于成人,肝包虫病患者的IgG亚类高于肺包虫病患者;IgG阴性小儿患者血清的CAg和CIC阳性率分别为40%及26.67%。结论:抗包虫总IgG抗体表达水平低下,抗体表达各类不同及循环免疫复合物的形成,是造成包虫病患者IgG抗体反应阴性的主要原因。而寄生包虫囊的退行变性也可影响抗体的免疫应答水平。IgGl IgA IgM抗体联合检测可提高包虫病人免疫诊断的敏感性。 相似文献
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目前临床应用的HP抗体测定试剂均为测定抗HP高分子量细胞相关蛋白的IgG或IgA抗体。采用酶联免疫方法测定。临床应用的结果表明这一方法与常规活检标本病理染色及培养方法之间有较好的相关性,敏感性与特异性较高,部分IgG阴性的患IgA阳性。另外,血清抗体滴度测定可以监测治疗的结果。 相似文献
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目的检测类风湿关节炎(RA)患者血清中不同亚型的抗环瓜氦酸肽(CCP)抗体,探讨其在RA中的相关临床意义。方法采用ELISA法检测135例RA患者、53例非RA的其他结缔组织病(CTD)患者和45例健康体检者血清中抗CCP抗体-IgA、IgG和IgM,并用比浊法检测类风湿因子(RF)-IgM。结果RA患者血清中的抗CCP抗体-IgA、IgG、IgM的检出率分别为57.8%、73.3%、30.4%,均高于CTD组和健康对照组,差异均有统计学意义(均P〈0.05)。RA患者的RF-IgM阳性者中的抗CCP抗体-IgA、IgG、IgM的阳性检出率均高于RF-IgM阴性者(P〈0.05)。抗CCP抗体一IgA、IgG亚型在≥24个月、7~23个月病程组中的阳性率高于≤6个月组(P〈005),但≥24个月与7~23个月阳性率的比较差异无统计学意义(P〉005)。抗CCP抗体一IgM在≥24个月的阳性率低于≤6个月组(P〈005),但7~23个月与≥24个月、≤6个月病程组间的阳性率比较差异均无统计学意义(均P〉0.05)。抗CCP抗体一IgA、IgM对RA的诊断敏感度均低于抗CCP抗体-IgG,差异均有统计学意义(均P〈0.05);而抗CCP抗体-IgA、IgM的诊断特异度分别与抗CCP抗体-IgG的比较差异均无统计学意义(均P〉0.05)。结论RA患者血清中存在不同亚型的抗CCP抗体,抗CCP抗体-IgA、IgM在RA诊断中与IgG亚型有相似的诊断特异度。 相似文献
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郭新瑛 《中国煤炭工业医学杂志》2003,6(12):1145-1147
目的 研究幽门螺杆菌感染与胆石形成的关系。方法 采用酶链免疫法(ELASA)对胆结石患者血清及胆汁免疫球蛋白IgG、IgA、IgM标本进行检测,对血清学及胆汁IgG均阳性患者的胆汁,采用免疫印迹法(Western Blot)进行幽门螺杆菌感染相关蛋白检测。结果 50例患者血清学阳性35例(70.0%),胆汁阳性29例(58.0%)。血清学及胆汁均阳性24例(480%)。血清学及胆汁均阳性24例中,Cag A检出17例(70.8%),Vac A检出11例(45.8%)。Ure B及Ure A分别为18例(75.0%)和16例(66.7%)。同时检出幽门螺杆菌细胞空泡毒素(Vac A)相关亚单位37kD、35kD糖蛋白。结论 胆汁中存在多种幽门螺杆菌感染相关蛋白,并可能参与胆结石的形成。 相似文献
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Toyonaga A Okamatsu H Sasaki K Kimura H Saito T Shimizu S Fukuizumi K Tsuruta O Tanikawa K Sata M 《The Kurume medical journal》2000,47(1):25-30
We conducted an epidemiological study to investigate the relation of food intake to Helicobacter pylori (H. pylori) infection in an area endemic for H. pylori. In this study, 365 subjects, 104 men and 261 women, were randomly selected from 7,389 adult (over age 20) inhabitants of town A, Japan. The prevalence of immunoglobulin G (IgG) class antibody to H. pylori (anti-H. pylori) was 83.7% and the prevalence of anti-H. pylori increased with age significantly (P < 0.05). Subjects with anamnesis of gastritis, gastroduodenal ulcer and gastric cancer tended to have a higher anti-H. pylori positive ratio (93.5%) than those without (81.0%). But there was no relationship between anti-H. pylori prevalence and sex, blood type, smoking or drinking habits. Daily intake of foods by food groups, nutrients and the concentrations of serum ingredients were compared between 37 anti-H. pylori-positive and 40 negative subjects selected from 365 inhabitants by matching up according to sex and age. The daily intake of cereals, potatoes and starches, and milks tended to be higher in positive than negative subjects, while the daily intake of algae and tea appeared to be a little higher in negative than in positive subjects. The daily zinc intake of antibody-positive subjects was significantly higher (P < 0.05) than in antibody negative subjects. On the other hand, the daily iron intake in negative subjects was significantly higher (P < 0.05) than in positive subjects. The serum concentrations of copper, zinc, and vitamin E tended to be higher in positive than negative subjects. But there were no significant differences in serum ingredients concentrations between antibody negative and positive subjects. Our findings suggest that iron and zinc intakes may effect on H. pylori infection. 相似文献
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目的探讨幽门螺杆菌(H.pylori,HP)感染及根除对十二指肠球部溃疡患者IL-18水平的影响,同时检测抗HP治疗前后抗体水平的变化.方法40例HP阳性的十二指肠球部溃疡患者,作为实验组随机分成治疗组和对照组各20例,并以20例健康体检者为体检组,观察近期疗效及HP根除情况,同时ELISA法检测HP-IgG及IL-18水平。结果HP阳性十二指肠球部溃疡患者外周血清IL-18及HP-IgG水平显著高于体检组,抗HP治疗后十二指肠球部溃疡患者的IL-18、HP-IgG抗体水平均有明显下降。结论抗HP治疗后IL-18水平的下降在十二指肠球部溃疡的治疗过程中发挥重要作用。 相似文献
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目的通过测定手足口病(HFMD)患儿机体血清炎症细胞因子如肿瘤坏死因子-α(TNF-01)、白细胞介素-6(IL-6).免疫球蛋白(IgA、IgG、IgM)及补体C3、C4水平,以探讨HFMD患儿体液免疫与HFMD的关系。方法收集舟山医院妇幼保健院区2010年6月-2012年12月HFMD患儿50例,其中30例肠道病毒71(EV71)感染呈阳性,20例呈阴性。另选取同期20例健康体检儿为对照。血清免疫球蛋白如IgA、IgG,IgM和补体C3、C4水平采用放射性免疫扩散法测定,血清TNF-α、IL-6采用化学发光免疫分析法进行测定。结果EV71阳性感染的HFMD患儿血清TNF-α、IL-6、IgA、IgG、IgM及补体C3、C4水平依次为(66.0±11.2)ng/L、(44.3±7.7)ng/L、(0.50±0.37)g/L、(8.09±3.55)g/L、(1.47±0.70)g/L、(0.96±0.51)g/L、(0.21±0.07)g/L,与其他肠道病毒引起HFMD相应指标水平比较,差异无统计学意义(t=0.462、0.734、0.209、0.125、0.259、0.209、0.833,均P〉0.05),但EV71阳性感染组血清TNF—α、IL-6、IgA、IgG、IgM和补体C3、C4水平与对照组比较,差异均有统计学意义(t=20.398、19.496、4.319、2.756、2.379、2.146、3.148,均P〈0.05),其中IgA、IgG和补体C3、C4水平较对照组明显降低,而IgM、TNF—α、IL-6则明显升高。结论EV71感染HFMD患儿的体液免疫功能紊乱.TNF—α、IL-6参与了HFMD的病理过程,可为HFMD的临床诊断、疗效观察及预后判断提供一定的理论依据。 相似文献
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Abdiev S Ahn KS Khadjibaev A Malikov Y Bahramov S Rakhimov B Sakamoto J Kodera Y Nakao A Hamajima N 《Nagoya journal of medical science》2010,72(3-4):167-172
Recent studies have reported that Helicobacter pylori (H. pylori) persistent infection and gastric atrophy development were associated with genetic polymorphisms of cytokines. This study aimed to determine possible associations of host genotypes with the seropositivity of anti-H. pylori IgG and anti-CagA IgG, as well as gastric atrophy measured with serum pepsinogens (PG) among an Uzbek population. Subjects were 84 patients with peptic ulcer disease, 35 with other miscellaneous diseases, and 48 healthy persons, for a total of 167 participants. Using a polymerase chain reaction with confronting two-pair primers, their DNA was genotyped for polymorphisms of interleukins (IL) (IL-1B C-31T, IL-2 T-330G, IL-4 C-33T, IL-8 T-251A, IL-10 T-819C, and IL-13 C-1111T) and tumor necrosis factor A (TNF-A) (C-857T and T-1031C). Among 167 participants, 124 (74.9%) were anti-H. pylori IgG seropositive, 142 (85.6%) were anti-CagA IgG seropositive, and 44 (26.3%) exhibited gastric atrophy (PG1 < 70 ng/ml and PG1/PG2<3). The adjusted odds ratio (OR) of IL-4 -33CT for anti-H. pylori IgG seropositivity was significant; OR = 2.33 (95% confidence interval (CI), 1.04-5.19), relative to -33CC. In addition, those with TNF-A-1031TC had a significantly increased risk for anti-H. pylori IgG seropositivity; OR = 2.82 (95% CI, 1.05-7.57), relative to -1031TT. No alleles were associated with the risk of anti-CagA IgG seropositivity or gastric atrophy. The significant associations with cytokine polymorphisms indicated that genetic traits might play a role in the persistent infection of H. pylori among Uzbeks. In addition to confirming the above associations, lifestyle interactions with the genotypes also remain to be elucidated. 相似文献
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目的:了解就诊及体检人群中幽门螺杆菌(HP)感染状况,探讨HP感染与相关疾病的关系。方法:采用金标免疫斑点法对3996人进行血清抗HP-IgG检测,还对其中2259人检测了抗HP-IgM。结果:(1)3996例中血清抗 HP-IgG总阳性率为76.0%;2259例 HP-IgM阳性率为41.2%。(2)HP-IgG、HP-IgM在性别间无显著性差异(P>0.05)。(3)年龄因素对HP-IgG和HP-IgM阳性率有显著(P<0.05)和极显著(P<0.001)影响。(4)HP感染存在家庭聚集倾向。(5)血清HP抗体阳性率升高的疾病:大于28 d小儿,消化系统疾病组较健康对照组,抗HP-IgM阳性率存在显著性差异(P<0.05);在成人中胃肠道疾病组与健康体检组抗HP-IgG阳性率相比,有极显著性差异(P<0.001);肝、胆、胰疾病、循环系统疾病组与健康体检组比较有显著性差异(P<0.05)。结论:HP感染与多种胃肠道、肝胆胰及循环系统疾病有关;人群初次感染始于婴幼儿期,且受家庭因素影响。 相似文献
18.
肺炎衣原体感染与动脉粥样硬化的关系 总被引:1,自引:0,他引:1
目的研究肺炎衣原体(Cpn)感染与动脉粥样硬化(AS)关系和Cpn特异性抗体检测的临床意义.方法采用间接免疫荧光试验,对67例冠心病(CHD)、32例心肌梗死(MI)患者和40名正常人(对照组)的血清进行了Cpn特异性IgG、IgA、IgM抗体的检测.结果显示Cpn IgG抗体的阳性率患者组与对照组之间差异无显著性(P>0.05),Cpn IgA抗体的阳性率患者组高于对照组(P<0.01);Cpn IgG 、IgA抗体滴度和几何平均滴度患者组高于对照组(P<0.01).结论 Cpn 抗体滴度有助于疾病的诊断;持续高滴度的Cpn IgG、IgA抗体可作为Cpn 慢性感染的重要指标;慢性Cpn感染是AS发生、发展的重要危险因素. 相似文献
19.
Mohammad Farhadi Samileh Noorbakhsh Azardokht Tabatabaei 《Medical journal of the Islamic Republic of Iran》2013,27(2):77-82
Objective
Searching the H. pylori -DNAs in adenoid and IgA, IgG antibodies in serum of children.Methods
A cross- sectional study had done upon 53 children (Mean age 8 ±1.9 years) with adenoid surgery in ENT and Pediatric Department of Rasul Akram Hospital (2008-2010). Of 53 cases with adenoid surgery, 40 cases had rhino sinusitis (in sinus CT scan). The resected adenoid tissues (n=40) centrifuged and homogenized and its DNAs extracted and searched for H. pylori- DNAs by qualitative PCR. Serum H. pylori antibodies (IgG &IgA ELISA) calculated quantitatively. Chi square values (p < 0.05) calculated for all categorical variables. The agreement between serologic test and PCR was assessed by the calculation of kappa statistic.Results
Positive PCR for H. pylori detected in 15%of cases; regardless of sex and age of cases. Positive IgA: 17.5% (7/40) and IgG: 20% (8/40) respectively. All cases with positive H. pylori- IgG were female; p=0.003 and meaningful differences in mean age of cases observed for positive IgA (p=0.001) and IgG (p=0.01). Poor agreement observed between positive PCR and serum IgG&IgA (Fisher''s Exact test=0.3; 0.5).Conclusion
Positive PCR in adenoid tissue (15%) was very close to positive serum IgA (15%) but without any agreement for each case. The H. pylori infection may have a relative role at least in 15% of children with adenoid surgery. Chronic sinusitis and ear infection might be added to infected adenoid tissue as a reservoir for bacteria. The search by specific culture may elucidate better the role of H. pylori infections in both gastric and adenoid tissues. The decision for use of antibiotics to eradicate the H. pylori infection in recurrent or chronic adenotonsillar infections (with rhinosinusitis) before adenoid surgery needs Randomized Control Trial (RCT) studies. Drug of choice for eradication of H. pylori dependent to antibiotic sensitivity test in each country. 相似文献20.
目的获得重组幽门螺杆菌(H.pylori)脂蛋白(rLpp20),并研究其在血清学诊断中的应用价值。方法应用重组质粒pGEX-6P.2/Lpp20在大肠杆菌(E.coli)BL21中表达,并进行GST亲和层析纯化,用Western.blot检测纯化产物(rLpp20)的免疫反应性;以rLpp20为包被抗原建立ELISA法对200份患者血清标本进行特异性IgG抗体检测,并与幽门螺杆菌-IgG抗体检测试剂盒法进行比较。结果成功表达的rLpp20(43000Mr),纯化产物可被幽门螺杆菌多克隆抗体及阳性血清识别;成功建立了以rLpp20为包被抗原的间接ELISA法,其灵敏度为91.0%,特异度为100%。与试剂盒法比较,两者的符合率为95.5%。结论Lpp20具有良好的免疫反应性,能与幽门螺杆菌阳性血清特异性结合,有望应用于幽门螺杆菌血清学诊断。 相似文献