深圳特区变应性鼻炎患者螨与蟑螂过敏原体外检测与分析

目的探讨深圳特区变应性鼻炎患者血清中螨和蟑螂过敏原特异性免疫球蛋白E(sIgE)的情况及其相互关系。方法采用酶联免疫吸附法(ELISA)对该院1998~2010年就诊于耳鼻咽喉科门诊的1 935例患者进行血清过敏原体外检测。结果在变应性鼻炎患者中,对尘螨过敏的具有极高的阳性率,蟑螂过敏也有较强的阳性率,血清中的sIgE值尘螨显著高于蟑螂(P<0.01)。结论尘螨和蟑螂均为引起变应性鼻炎最主要的过敏原之一,尤其是尘螨,预防和治疗研究工作的重点应当是这些过敏原。     

Indoor Allergen Levels and Household Distributions in Nine Cities Across China

Objective Chinese allergic subjects have high levels of sensitization to house dust mite (HDM) and other indoor allergens. This study quantifies common indoor allergen levels in Chinese households.
Methods Dust samples were collected from nine cities. Major allergens Der p 1 and Der f 1 from Dermatophagoides pteronyssinus and D. farinae, and specific antigens of Blomia tropicalis, Tyrophagus putrescentiae, Acarus siro, and cockroach species Blattella germanica and Periplaneta americana were measured by ELISA.
Results HDM allergens were found in dust samples from bedding in 95% of the Chinese households. The median levels varied from <0.006 to 9.2 μg/g of dust, depending on the city. The percentages of households having HDM allergen levels associated with the risk of developing allergy sensitization and asthma were 65% and 25%, respectively. Specific antigens of the storage mite and cockroach were only found in samples from the southern and tropical regions of China. Levels of mite allergens were generally higher in samples from bedding compared to samples from the living room, even for storage mites, whereas levels of cockroach antigens were higher in the living room samples.
Conclusion HDM allergens are present in bedding dust samples from most Chinese households. Cities in southern and central China have relatively high levels of HDM major allergens compared to cities in northern and western China. Antigens of storage mites and cockroaches are not as common as HDM allergens.     

五岁以下哮喘儿童变应原的检测及临床意义

目的了解年幼儿哮喘常见变应原,为有效避免变应原及早期干预治疗提供依据。方法选择在海南省人民医院儿童哮喘防治中心治疗的5岁以下哮喘儿童468例,使用丹麦爱尔开-阿贝优公司的吸入组点刺试剂进行皮肤点刺试验,对其中251例哮喘患儿定量检测血清中变应原特异性IgE抗体水平。结果 468例哮喘患儿体内过敏原（皮肤点刺试验）阳性率为66%,尘螨皮试阳性率最高且强阳性率（＋＋＋—＋＋＋＋）也最高,其中屋尘螨及粉尘螨分别为87.4%及86.3%,热带螨为66.3%,德国小蠊及美洲大蠊分别为26.7%及19.9%。动物皮毛中,以狗毛为主（20.1%）,猫毛次之（12.4%）。251例体外变应原检测阳性率为57.8%,其中仍以屋尘螨阳性率（63.25%）最高,粉尘螨（58.6%）次之,狗上皮为15.9%。食物组中蛋清为9.3%、花生为4.3%、苹果为3.1%,分居前3。结论哮喘儿童皮肤点刺试验有较高的变应原检出率,屋尘螨、粉尘螨、热带螨是海南儿童哮喘最常见的吸入变应原,蟑螂、狗毛、猫毛等变应原也不容忽视。食入性过敏原检出率低,其对儿童哮喘的影响远低于吸入变应原。     

郑州地区成人自报过敏性鼻炎患者吸入过敏原分析

  目的  了解郑州地区成人自报过敏性鼻炎(allergic rhinitis, AR)患者吸入过敏原致敏谱,分析不同年龄、性别及年份间阳性致敏原的差异,为AR的临床诊治提供参考。  方法  采用17种吸入过敏原试剂对2019年1月—2021年12月就诊于河南省人民医院过敏反应科门诊且自报为AR的成人患者2 305例进行皮肤点刺试验(skin prick test, SPT),比较不同性别、年龄、年份致敏原分布情况,并对阳性率进行分析。  结果  2 305例AR患者SPT阳性率为77.09%,常合并多种过敏原致敏,对4种及以上过敏原阳性者1 137例(49.33%);过敏原阳性率前5位依次为粉尘螨(42.78%)、户尘螨(38.61%)、梧桐花粉(35.27%)、蒿属花粉(34.27%)、葎草花粉(31.63%);18~30岁年龄组过敏原SPT阳性检出率达峰值(91.84%),阳性率随年龄增长而下降;不同性别间粉尘螨、户尘螨、油菜花、葎草花粉SPT阳性率比较差异有统计学意义(均P＜0.05);2019—2021年室内吸入过敏原粉尘螨、户尘螨阳性率呈逐年升高趋势,季节性吸入过敏原梧桐花粉、蒿属花粉、葎草花粉阳性率呈逐年下降趋势。  结论  AR患者常合并4种及以上吸入过敏原致敏;18~30岁SPT检出率达峰值;粉尘螨、户尘螨、梧桐花粉、蒿属花粉、葎草花粉是郑州地区成人自报AR患者最常见的致敏原。      

变态反应性疾病患者螨和蟑螂过敏原体外检测分析

目的探讨变态反应性疾病患者血清中螨和蟑螂过敏原特异性免疫球蛋白E(SIgE)的情况以及它们的相互关系分析。方法采用荧光酶联免疫吸附法,对我院2004年10月至2005年10月就诊于呼吸科和变态反应科门诊的199例患者,检测其血清中螨和蟑螂的SIgE。结果在变态反应性疾病的患者中,对于螨类过敏的,包括屋尘螨(d1)、粉尘螨(d2)和热带无爪螨(d201)均为儿童组比成年组的阳性率高,同时具有较高的强阳性率;对于热带无爪螨过敏的患者,一般都是在4级以下,极个别具有强阳性且均为儿童;对于蟑螂过敏的,所有患者均在4级以下;对于同时检测尘螨和蟑螂的患者,其血清中的SIgE值均为各螨类显著高于蟑螂的值(P<0.01)。结论尘螨和蟑螂均为变态反应性疾病的重要过敏原,尤其是尘螨;对于尘螨和蟑螂同时过敏的患者,尘螨的过敏程度均比蟑螂高。     

Allergen micro-array detection of specific IgE-reactivity in Chinese allergy patients

Background  Allergen micro-arrays are powerful tools for screening of serum IgE-reactivity. In this study allergen micro-arrays were used to identify dominating IgE-binding allergens and cross-reactivity patterns among selected Chinese allergy patients.

Methods  The study was conducted using patient sera from the cities of Guangzhou, Nanjing, Chengdu and Shenyang. In total 100 sera with Dermatophagoides pteronyssinus (Der p) specific IgE-levels higher than 50 kU/L were selected for testing against 103 individual allergens.

Results  Among 100 selected patients, 95% showed IgE-reactivity towards house-dust mite allergens Dermatophagoides farinae (Der f) 1, Der f 2 and Der p 2 and 94% were IgE positive against Der p 1, and 60% of sera contained IgE reacting against allergen Euroglyphus maynei (Eur m) 2. IgE against cat allergen, Felisdomesticus (Fel d) 1, was seen in 20%. Only 2% showed specific IgE-reactivity to Der p 10, a panallergen belonging to the tropomyosin family. Serum IgE-reactivity towards other allergens was in general low. IgE-reactivity against pollen allergens showed geographic differences.

Conclusions  This study clearly confirms that group 1 and group 2 are major allergens of house dust mites. These selected house-dust mite allergy patients are close to being mono-sensitized. Der p 10 is not an important allergen for cross-reactivity. Specific IgE-sensitization towards pollen allergens is low in southern China compared to other regions. The prevalence of food and stinging insect allergens known to give rise to IgE-mediated cross-reactivity is 2% or less.

     

汕头地区支气管哮喘患儿过敏原状况的调查分析

目的：通过对汕头地区哮喘患儿过敏原的分布状况进行调查分析,为本地区儿童哮喘的预防和治疗提供依据。方法：应用屋尘螨、粉尘蟎等18种常见过敏原的标准化提取液对579例支气管哮喘患儿进行皮肤过敏原点刺试验,记录结果并加以分析。结果：过敏原检测总阳性率为79.1（,其中阳性检出率前两位的过敏原依次为粉尘螨、屋尘螨。结论：汕头地区支气管哮喘患儿主要的过敏原为粉尘螨、屋尘螨等。     

青浦地区变应性鼻炎患者吸入变应原检测分析

目的了解青浦地区变应性鼻炎患者吸入变应原分布情况,为青浦地区变应性鼻炎的流行病学研究和防治提供重要依据。方法回顾分析583例变应性鼻炎患者对14种吸入变应原进行皮肤点刺试验的临床资料,并比较两组年龄段及吸入变应原阳性率在分布上的差异。结果在青浦地区变应性鼻炎患者中,最主要的吸入变应原是屋尘螨(72.6%)、粉尘螨(68.8%)、热带螨(41.3%),其次是狗毛(12.7%),并且阳性率随年龄的增长有明显下降趋势。结论屋尘螨、粉尘螨是青浦地区变应性鼻炎最常见的变应原。皮肤点刺试验(Skin Prick Test,SPT)有助于发现变应原,为免疫治疗提供重要依据。     

海口市儿童哮喘变应原流行病学调查研究

目的 了解海口市儿童哮喘常见变应原状况.方法 2012年9~12月本研究采取整群抽样的流行病学方法调查海口市0~14岁儿童10 241名,确诊哮喘患儿324例,对所有哮喘患儿进行过敏史问卷调查及皮肤点刺试验和血清过敏原特异性IgE (SIgE)抗体检测.结果 324例哮喘患儿中224例(69.1%)过敏史阳性,其中湿疹91例(28.1%),食物过敏64例(19.8%),药物过敏28例(8.6%),香烟过敏18例(5.6%),装修气味过敏19例(5.9%),宠物过敏13例(4.0%),油烟过敏11例(3.4%),花粉过敏9例(2.8%).皮肤点刺阳性率为83.0%(269例),其中屋尘螨及粉尘螨阳性率分别为90.1%(292例)及89.2%(289例),热带螨为85.5%(277例),蟑螂19.6%(64例),霉菌3.4%(11例),花粉3.4%(11例),猫毛11.7%(38例),狗毛25.3%(82例).吸入性过敏原IgE阳性率为72.2%(235例),以尘螨为最高(69.8%,226例);食入性过敏原IgE阳性率为28.7%(93例),牛奶占25.3%(82例),鸡蛋白占9.9%(32例),虾蟹占22.8%(74例),热带水果占9.0%(24例),鱼占8.3%(27例).不同年龄组哮喘患儿吸入性过敏原特异性IgE阳性率比较显示,3~7岁组屋尘螨、蟑螂阳性率高于0~3岁组(P<0.05),7~14岁组屋尘螨、蟑螂、狗毛上皮、猫毛上皮阳性率高于0~3岁组(P<0.05).结论 海口市儿童哮喘常见变应原以屋尘螨、粉尘螨和热带螨为主,狗毛、蟑螂及猫毛比例也较高.食入性过敏原以牛奶、鸡蛋白多见,且虾蟹、热带水果致敏对本地区儿童哮喘的影响也不容忽视.     

过敏原检测对儿童支气管哮喘早期干预的意义

[目的]了解儿童支气管哮喘的过敏原谱及相关因素。[方法]采用酶联免疫过敏原体外检测法检测支气管哮喘患儿血中的过敏原特异性IgE（sIgE）。[结果]支气管哮喘患儿168例过敏原的总阳性率为87．5％（147／168）。吸入组阳性率较高，其中尘螨的阳性率最高，为77．98％（131／168），其他依次为蟑螂17．26％，霉菌16．67％，花粉16．07％，屋尘13．1％。食物组总阳性率51．19％，其中牛奶和鱼、虾、蟹的阳性率分别为22．62％和21．43％。食物组以弱阳性居多。哮喘儿童中尘螨的阳性率与年龄有关。〈3岁组阳性率56．25％（27／48），3～6岁组92．31％（48／52）〉6岁组82．35％（56／68）。血清总IgE水平以尘螨阳性者最高，其他过敏原阳性组次之，未检出过敏原组最低。过敏原阳性组哮喘儿童年龄平均为5．79±0．77岁，略大于过敏原阴性哮喘儿童（3．45±2．11岁）。[结论]儿童支气管哮喘大部分与过敏有密切关系。尘螨仍是诱发儿童支气管哮喘的主要吸入变应原，其过敏在婴幼儿期已非常常见，在学龄前期达到高峰，以后持续在较高水平上。说明儿童支气管哮喘的早期预防重点应在生命早期，尤其对于早期血清IgE明显升高者，更应采取积极的早期干预措施。     

荨麻疹患者血清特异性IgE的测定与分析

目的 :探讨由 Ig E介导的 型变态反应在荨麻疹发病中的意义及考察 MAST- CAL检测法的临床应用价值。方法 :采用 MAST- CAL法 (多种抗原光化学分析法 )检测了 180例荨麻疹患者血清特异性 Ig E水平 ,分析各种致敏原在荨麻疹发病中的意义。结果 :180例病人中 ,吸入组检出阳性 62例 ,阳性率 34 .4 4 % ,常见的吸入过敏原为粉尘螨、屋尘螨、蟑螂 ;食物组检出阳性为 53例 ,阳性率为 2 9.4 4 % ,最常见的食物过敏原为黄豆、吞拿鱼。结论 :由 Ig E介导的 型变态反应仅占荨麻疹病因的一部分 ,南方地区常见的吸入过敏原为粉尘螨及屋尘螨。 MAST- CAL检测法在检测荨麻疹患者血清特异性 Ig E水平上可提供可靠的实验结果。     

Comparison and Evaluation of Several Dermatophagoides Pteronyssinus Allergen Extracts for Skin Prick Test

Objective To evaluate the significance of several Dermatophagoides pteronyssinus allergen extracts for skin prick test （SPT） in patients allergic to Dermatophagoides pteronyssinus. Methods Two hundred and nineteen patients enrolled in Peking Union Medical College Hospital underwent SPT and serum specific IgE assay to detect the Dermatophagoides pteronyssinus allergen. Three kinds of house dust mite allergen extracts were used for SPT, including the Dermatophagoides pteronyssinus extract prepared by our laboratory （group A）, standardized Dermatophagoides pteronyssinus extract （group B）, and mixed extracts of Dermatophagoides pteronyssinus and Dermatophagoidesfarinae （group C）. Human serum specific IgE result was regarded as the reference standard for diagnosis of Dermatophagoides pteronyssinus allergy. The receiver operating characteristic （ROC） curve was used to evaluate the diagnostic performance of SPT with the extracts of three groups.Results SPT results showed that the median wheal diameter of group A, group B, and group C was 0.43, 0.35, and 0.28 cm, respectively, with significant difference among three groups （P〈0.05）. The difference was significant between group A and B （P〈0.01） as well as group A and C （P〈0.01）, but not between group B and C （P〉0.05）. There was no local urticaria or systemic allergic reactions following the procedure of SPT. Local reaction was observed in 5 patients and delayed reaction was in 2 patients of group A. As for group B and C, local reaction occurred in 3 cases and delayed reaction in 2 cases in each group. The area under ROC curve of SPT with extract in group A, group B, and group C was 0.765, 0.801, and 0.782, respectively. Based on the detection results of serum specific IgE, the sensitivity of SPT in diagnosis of Dermatophagoides pteronyssinus allergy with extract of group A, group B, and group C was 92.4%, 87.0%, and 81.5%, and the specificity was 60.6%, 73.2%, and 74.8%, respectively. Conclusion The Dermatophagoides pteronyssinus extract for SPT prepared by our laboratory offers good sensitivity and specificity comparable to commercially available allergen extracts, and it may be an appropriate candidate for clinical screening and diagnosis of Dermatophagoides pteronyssinus allergy.     

铜陵地区儿童呼吸道过敏性疾病的变应原谱研究

目的分析铜陵地区儿童呼吸道过敏性疾病的变应原谱特征,为本地区儿童常见过敏性疾病的防治提供科学依据。方法选择患有儿童哮喘和(或)变应性鼻炎、特应性皮炎等患儿50例,男性31例,女性19例,平均年龄(6.32±3.13)岁。所有对象均选用20种变应原进行皮肤点刺试验,以组胺作阳性对照,生理盐水作阴性对照。结果铜陵地区吸入性变应原以粉尘螨的阳性率最高为84%,其次是户尘螨阳性率82%、猫毛和狗上皮阳性率54%。食入性过敏原以海虾的阳性率最高62%,其次为牛肉和鸡蛋黄阳性率58%、花生阳性率56%。儿童哮喘、变应性鼻炎、特应性皮炎患儿均以尘螨类过敏最为常见。结论过敏性因素是儿童过敏性疾病的重要诱发因素,铜陵地区最常见的变应原是尘螨类、狗猫皮毛类和海虾、牛肉,可通过皮肤点刺试验明确过敏原,有助于对儿童常见呼吸道过敏性疾病进行早期诊断及早期干预。     

Indoor mite allergen levels, specific IgE prevalence and IgE cross-inhibition pattern among asthmatic children in Haikou, southern China

Background  Haikou locates in tropical island with unique mite propagation. The aim of this stuy is to determine mite allergens levels in Haikou, and to investigate the prevalence of mite specific IgE-sensitization and IgE cross-reactivity between house dust mites. 

Methods  Allergen and antigen concentrations against six mite species were tested by enzyme-linked immunosorbent assay (ELISA). Specific IgE concentrations and cross-inhibitions were measured with ADVIA Centaur^®.

Results  Allergen or antigen Dermatophagoides pteronyssinus (Der p 1), Blomia tropicalis (Blo t ) and Tyrophagus putrescentia (Tyr p) were detected in dust samples. Dermatophagoides farinae (Der f 1), Lepidoglyphus destructor (Lep d 2), and Acarus siro (Aca s ) were found in very few samples. Specific IgE tests showed high prevalence of sensitizations against all tested mites with high IgE levels to Der p, Der f, and Blo t. Storage mites, Blo t, Tyr p, Lep d, and Aca s, could inhibit Der p from 0 to 50%. Storage mites could inhibit Der f between 30% and 100%. Der p IgE could be inhibited by Der f with up to 90%, and vice versa. Der p could inhibit Blo t from 40% to 80%. Blo t was able to fully inhibit IgE binding to Lep d, Tyr p, and Aca s compared to partial inhibition by Der p.

Conclusions  Der p is the dominating mite and has the highest specific IgE prevalence among asthmatic children. Blo t represents an important source of storage mite sensitization and some patients may be independently sensitized to both Der p and Blo t. High prevalence of sensitization to Der f may be due to IgE-mediated cross-reactivity with Der p and Blo t.

     

支气管哮喘血清IgE和ECP的水平及相关性分析

目的探讨支气管哮喘血清IgE和ECP的水平,并分析IgE和ECP的相关性.方法对象为54例支气管哮喘患者和20例健康正常人,采用酶联免疫法测定其血清IgE和ECP的水平.结果支气管哮喘血清TIgE和ECP水平明显高于正常对照组(P<0.01);血清中TIgE含量与屋尘螨SIgE水平存在直线相关(r=0.296,P<0.05);血清中的TIgE与ECP和屋尘螨SIgE与ECP之间并无明显相关性.结论屋尘螨是引起支气管哮喘的重要变应原;血清TIgE和SIgE与ECP均无相关,初步解释为IgE表示患者变应体质的水平,但不能反映气道炎症的病理过程;而ECP水平可以客观地反映变应性哮喘患者体内炎性细胞激活的程度,可作为哮喘气道炎症监测的指标.     

1491例变态反应性疾病患者过敏原检测结果分析

目的调查分析常州地区变态反应性疾病患者常见过敏原的分布情况,比较不同年龄阶段、不同季节的过敏原有无差异。方法选取本院2016年1月～2018年1月收治的1491例变态反应性皮肤病患者,采用16种过敏原对患者进行皮肤点刺试验,生理盐水作阴性对照,以组胺作阳性对照。结果 1491例变态反应性皮肤病患者常见过敏原阳性率从高到低依次为:粉尘螨89.67%、户尘螨86.38%、油菜花7.11%、葎草花粉6.37%蟑螂4.76%、猫毛3.76%。过敏原的季节性来分析:粉尘螨、户尘螨、夏季最高;春天比其他三个季节低;各种花粉在夏季最低,蟑螂夏季最高。粉尘螨、户尘螨、猫毛、狗上皮、狗毛季节性变化不是太明显。粉尘螨、户尘螨、猫毛、牛奶、鸡蛋黄未成年组高于成年组(P0.05)。葎草花粉、油菜花粉、蟑螂成年组高于未成年组(P0.05)。过敏原在同年龄阶段的男女中间的差异不明显(P0.05)。结论在常州地区,最主要的过敏原为粉尘螨、户尘螨、油菜花,过敏原在不同季节、不同年龄阶段分布不同,为本地区常见变态反应性疾病的防治提供科学依据,皮肤点刺试验用于明确变态反应性疾病致敏原因安全有效。     

尘螨变应原皮内试验与血清IgE检测结果的相关性

目的 探讨变应性鼻炎粉尘螨及户尘螨变应原体内检测与体外检测的相关关系.方法 选择变应性鼻炎患者34例, 体内检测采用皮内试验法, 体外检测采用血清特异性IgE检测法, 根据皮内试验结果分为:极强阳性组、强阳性组、中阳性组、阳性组及阴性组5个级别;应用Excel软件进行统计分析.结果 粉尘螨、户尘螨变应原皮内试验分别与体外血清lgE检测具有高度相关性;变应原皮内试验 (即户尘螨与粉尘螨的平均值) 与IgE检测具有高度相关性, 相关系数r=0.8532 (P<0.01, n=20) .结论 粉尘螨和户尘螨变应原皮内试验与体外血清lgE检测具有高度相关性, 临床上可以在患者条件允许的情况下先做皮内试验作为筛查, 然后再用体外检测进一步验证;对于高敏患者或其他不接受皮内试验的患者, 可直接进行体外检测.     

过敏性哮喘患者蟑螂抗原皮肤反应性调查

目的 对门诊蟑螂过敏性哮喘发病情况进行粗略判断,并以对黑胸大蠊与美洲大蠊抗原皮肤反应性作一比较。方法 回顾分析212例有14种抗原（皮肤点刺试验）资料的过敏性哮喘及过敏性鼻炎患儿蟑螂抗原皮试结果;总结34例成人过敏性哮喘患者上述两种蟑螂抗原皮试（皮内试验）结果。结果 有皮试资料的212例患儿中,蟑螂抗原皮肤点刺试验阳性61例,阳性率28．7％,占14种抗原结果的第4位。而就气传变应原相比较,排在屋尘螨及粉尘螨（均为89．6％）之后,居第3位;34例成人患者中,蟑螂抗原皮内试验阳性6例,阳性率17．7％。黑胸大蠊与美洲大蠊抗原皮试阳性结果一致。结论 蟑螂是门诊过敏性哮喘及过敏性鼻炎患儿常见致敏原之一;哮喘患者对黑胸大蠊与美洲大蠊,抗原反应性一致。     

过敏性鼻炎血清特异性IgE与皮肤点刺试验的关联性分析

目的评估血清特异性IgE检测与皮肤点刺试验在诊断过敏性鼻炎中的关联性。方法皮肤点刺试验中尘螨阳性的过敏性鼻炎患者82例,采用瑞典法玛西亚公司UniCAP100系统检测血清尘螨特异性IgE,包括屋尘螨及粉尘螨,统计分析二者的关联性。结果82例尘螨过敏患者中,55例尘螨血清特异性IgE检测结果大于或等于1级,特异性IgE水平与皮肤点刺试验结果的等级呈正相关(屋尘螨rs=0.58,粉尘螨rs=0.60)。不同年龄段患者的血清特异性IgE检测结果与皮肤点刺试验的相关系数随年龄的增长而增大,40岁以上患者二者的检测结果无相关性。结论尘螨过敏性鼻炎患者血清特异性IgE水平与皮肤点刺试验结果呈正相关,且随患者年龄变化而有所差异。诊断40岁以上患者时,推荐同时进行2种检测。     

居室粉螨抗原与螨过敏性哮喘相关性研究

目的:检测张家港市部分居室粉螨抗原及浓度,探讨其与螨过敏性哮喘发作的相关性。方法:收集过敏性哮喘患者及健康者居室床面、地面、家具、空调隔尘网及空气中的灰尘样本,用PCR法检测Der f1、Der f2、Der p1、Der p2阳性率,并用ELISA分别定量检测Der f1、Der p1及Der变应原含量。结果:共收集样本400份,过敏性哮喘患者与健康者居室Der f1、Der f2、Der p1、Der p2基因阳性率无显著性差异(P>0.05),但过敏性哮喘患者居室样本中这些变应原浓度>10μg/g者所占比例显著少于健康者(P<0.05)。对过敏性哮喘患者进行问卷调查结果显示,近期哮喘发作者Der f1、Der f2、Der p1、Der p2浓度>2μg/g所占比例明显高于近期哮喘未发作者(P<0.01)。结论:个体特异性是螨过敏性哮喘患者患病的重要因素,居室粉螨抗原浓度<2μg/g较安全。