固相萃取结合HPLC法快速测定血清丝裂霉素C含量

目的建立测定血清丝裂霉素C含量的方法.方法血清标本经固相萃取,以反相HPLC测定丝裂霉素C含量.流动相为水乙腈(8515),波长为365nm.结果浓度为5μg/L和100μg/L标准样本的批内变异分别为5.6%、4.5%,批间变异分别为8.7%、7.8%.浓度为5μg/L、100μg/L、200μg/L的标准样本平均回收率分别为91%、94%、97%.线性范围为1～200μg/L.结论该方法简便、敏感、准确、特异,可适于临床应用.     

RP-HPLC法同时测定人血清和脑脊液中苯巴比妥、苯妥英钠和卡马西平浓度

目的建立RP-HPLC法同时测定人血清和脑脊液中苯巴比妥（PB）、苯妥英钠（PHT）和卡马西平（CBZ）浓度的方法。方法色谱柱：Agilent ZORBAX SB-C18（250mm×4.6mm,5μm）;流动相：甲醇-乙腈-水（27.5∶27.5∶45）;流速：1.0mL.m in-1;检测波长：210nm;柱温：40℃;进样量：10μL。结果血清中PB、PHT、CBZ的线性范围依次为：2.64～52.75μg.mL-1、1.45～29.00μg.mL-1、1.04～20.80μg.mL-1;脑脊液中PB、PHT、CBZ的线性范围依次为：1.32～42.20μg.mL-1、0.73～23.20μg.mL-1、0.52～16.64μg.mL-1;血清中PB、PHT、CBZ的平均提取回收率分别为：93.17%、96.17%、94.67%,脑脊液中PB、PHT、CBZ提取回收率接近100%;日间、日内RSD均小于4%。结论本方法简单、快速、准确,适用于临床血及脑脊液浓度的检测。     

RP-HPLC法同时测定人血清和脑脊液中苯巴比妥、苯妥英钠和卡马西平浓度

目的建立RP-HPLC法同时测定人血清和脑脊液中苯巴比妥(PB)、苯妥英钠(PHT)和卡马西平(CBZ)浓度的方法。方法色谱柱:Agilent ZORBAX SB-C18(250mm×4.6mm,5μm);流动相:甲醇-乙腈-水(27.5∶27.5∶45);流速:1.0mL.m in-1;检测波长:210nm;柱温:40℃;进样量:10μL。结果血清中PB、PHT、CBZ的线性范围依次为:2.64～52.75μg.mL-1、1.45～29.00μg.mL-1、1.04～20.80μg.mL-1;脑脊液中PB、PHT、CBZ的线性范围依次为:1.32～42.20μg.mL-1、0.73～23.20μg.mL-1、0.52～16.64μg.mL-1;血清中PB、PHT、CBZ的平均提取回收率分别为:93.17%、96.17%、94.67%,脑脊液中PB、PHT、CBZ提取回收率接近100%;日间、日内RSD均小于4%。结论本方法简单、快速、准确,适用于临床血及脑脊液浓度的检测。     

在线镉柱还原—流动注射法测定水中硝酸盐氮和亚硝酸盐氮

目的探索一种测定水中硝酸盐氮和亚硝酸盐氮的简单快捷方法。方法利用在线镉柱还原—流动注射法,测定养殖海水、地表水、自来水、地下水等水样中的硝酸盐氮和亚硝酸盐氮。结果硝酸盐氮检出限为0.64μg/L,在20.0~2 000.0μg/L范围内呈理想线性关系,相关系数r=0.9999,相对标准偏差为0.97%,样品测定频率为60样/h,实际水样的加标回收率为90%~106%;亚硝酸盐氮的方法检出限为0.12μg/L,在2.0~200.0μg/L范围内呈理想线性关系,相关系数r=1.0000,相对标准偏差为0.35%,样品测定频率为90样/h,实际水样的加标回收率为92%~109%。结论在线镉柱还原—流动注射分析法简单快捷,测定结果满意。     

原发性癫痫患者苯妥英血清浓度监测

目的了解原发性癫痫患者血清苯妥英浓度与疗效的关系.方法用紫外分光光度法,测定苯妥英血药浓度,以10～20μg.ml-1为有效治疗浓度范围,并观察患者的症状及合并用药情况.结果测定结果低于10μg.ml-1 3例,占7.7%,高于20μg.ml-1 26例,占66.7%.检测结果以10～20μg.ml-1为有效范围,合格率为25.6%.结论对长期使用苯妥英的患者进行血药浓度监测,使之个体化用药,才能达到安全、有效的治疗目的.     

固相萃取结合HPLC法快速测定血清丝裂霉素C含量

目的建立测定血清丝裂霉素C含量的方法。方法:血清标本经固相萃取,以反相HPLC测定丝裂霉素C含量。流动相为水乙腈(8515),波长为365nm。结果:浓度为5μg/L和100μg/L标准样本的批内变异分别为5.6%、4.5%,批间变异分别为8.7%、7.8%。浓度为5μg/L、100μg/L、200μg/L的标准样本平均回收率分别为91%、94%、97%。线性范围为1～200μg/L。结论:该方法简便、敏感、准确、特异,可适于临床应用。     

高压微波消解原子荧光光谱法同时测定奶粉中痕量锡和硒

目的建立高压微波消解原子荧光光谱法同时测定奶粉中痕量锡和硒的方法。方法利用高压微波消解奶粉,在样品消解液中加入150 g/L硫脲-坏血酸作为预还原剂,用2%盐酸作为介质,加入2%的硼氢化钾溶液作为还原剂,采用原子荧光光谱法同时测定锡和硒元素。结果在最佳测试条件下,硒方法检出限为0. 3μg/L,测定相对标准偏差为1. 0%～2. 0%,样品加标回收率为96. 7%～99. 6%。锡方法检出限为0. 3μg/L,测定相对标准偏差为2. 7%～3. 4%,样品回收率为97. 0%～100. 6%。结论本法具有快速、准确、灵敏度高、精密度好等优点,用于奶粉中锡和硒的测定,结果满意。     

氢化物发生—原子荧光法同时测定茶水中砷和汞

目的 建立同时测定茶水中砷和汞的氢化物发生-原子荧光法.方法 采用硝酸-高氯酸微机消解仪恒温消解.结果 砷和汞的线性检测范围分别为0～50μg/L和0～2μg/L.方法 检出限分别为0.042μg/L和0.038μg/L,样品加标回收率分别为92.3%～102.0%、92.5%～98.8%.结论 本法适用批量样品消解,同时测定砷和汞含量的优点,方法简单、快速、灵敏度高、检出限低,节省试剂,结果可靠,仪器性能稳定,适合茶水中痕量砷、汞的检测分析.     

原发性癫痫患者苯妥英血清浓度监测

目的　了解原发性癫痫患者血清苯妥英浓度与疗效的关系。方法　用紫外分光光度法 ,测定苯妥英血药浓度 ,以 1 0～ 2 0μg.ml-1为有效治疗浓度范围 ,并观察患者的症状及合并用药情况。结果　测定结果低于 1 0μg.ml-13例 ,占 7.7% ,高于 2 0μg.ml-12 6例 ,占 66.7%。检测结果以 1 0～ 2 0μg.ml-1为有效范围 ,合格率为 2 5 .6%。结论　对长期使用苯妥英的患者进行血药浓度监测 ,使之个体化用药 ,才能达到安全、有效的治疗目的。     

血清中微量铍的火焰原子吸收法测定

本文叙述了火焰原子吸收法测定血清中微量铍的方法,研究了血清中各种无机离子对测定的影响。Ca~(2+)离子对铍的测定产生负干扰,当测定血清中铍时,则 Ca~(2+)的干扰可被血清中蛋白质等有机物消除。本法灵敏度为0.026μg/mL/1%,检测极限为0.019μg/mL。本法简便、快速、重复性好,也易于推广。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.