低能量激光配合牙周基础治疗对糖尿病合并慢性牙周炎患者龈沟液细胞因子及LPS、leptin的影响

目的: 分析低能量激光照射疗法（low level laser treatment, LLLT）配合牙周基础治疗对糖尿病合并慢性牙周炎（diabetes mellitus complicated with chronic periodontitis, DMCP）患者龈沟内细胞因子及LPS、Leptin的影响。方法: 将2016年1月—2017年1月间收治的80例DMCP患者依照随机信封法分为实验组及对照组,每组40例。对照组采用牙周基础治疗,实验组采用低剂量激光配合牙周基础治疗。治疗前及治疗后3个月检测患者口腔PD、SBI及CAL水平;采集患者空腹静脉血,检测血液中糖代谢指标;采集患者龈沟液,检测龈沟液中炎症因子水平。采用SPSS19.0软件包对数据进行统计学分析。结果: 经治疗后2组CAL、PD及SBI水平均显著降低（P<0.05）,治疗后实验组CAL、PD及SBI水平显著低于对照组（P<0.05）。治疗后2组患者血中FBG及HbA1c水平均显著降低（P<0.05）,治疗后实验组FBG及HbA1c水平显著低于对照组（P<0.05）。治疗后2组患者龈沟液中hs-CRP、IL-1β及TNF-α水平均显著降低（P<0.05）,治疗后实验组患者龈沟液中hs-CRP、IL-1β及TNF-α水平显著低于对照组（P<0.05）。治疗后2组患者龈沟液LPS显著降低,Leptin水平显著升高（P<0.05）,实验组龈沟液中LPS显著低于对照组,Leptin水平显著高于对照组（P<0.05）。结论: 低能量激光配合牙周基础治疗,可有效降低DMCP患者血糖水平及龈沟内LPS水平,提高Leptin水平,显著改善病灶区炎症反应,提高临床疗效。     

牙周基础治疗对龈沟液和血清内皮素、VEGF-A和TNF-α水平的影响

目的 观察非手术性牙周治疗对龈沟液和血清中内皮素（endothelin,ET）、血管内皮细胞生长因子A（vascular endothelial growth factor -A,VEGF-A）和肿瘤坏死因子α（tumor necrosis factor -α,TNF-α）水平的影响。方法 收集2017年10月—2018年6月于广州中医药大学深圳医院就诊的牙周炎患者57例及同期来院进行体检的43名健康人,分别作为牙周炎组与正常对照组。牙周炎组患者给予龈下深刮和根面平整治疗6周。检测2组受试者治疗前、后牙龈探诊出血（bleeding on probing,BOP）率、菌斑指数（plaque index,PI）、探诊深度（probe depth,PD）、临床附着水平（clinical attachment level,CAL）及牙龈指数（gingival index,GI）等牙周临床参数,以及龈沟液与血清中内皮素、VEGF-A和TNF-α水平,分析牙周炎患者治疗前龈沟液中ET水平与VEGF-A及TNF-α水平的关系。采用SPSS 21.0软件包对数据进行统计学分析。结果 正常对照组受试者BOP、PI、PD、CAL及GI等牙周临床参数均显著小于牙周炎组患者（P<0.05）;牙周炎组患者治疗前BOP、PI、PD、CAL及GI等牙周临床参数均显著大于治疗后（P<0.05）;治疗后,牙周炎组患者龈沟液与血清中ET、VEGF-A及TNF-α水平较治疗前显著下降（P<0.05）;且牙周炎组患者治疗前、后龈沟液与血清中ET、VEGF-A及TNF-α水平均显著大于正常对照组（P<0.05）;牙周炎患者治疗前龈沟液中ET水平与VEGF-A水平无显著相关性（P>0.05）,但治疗前龈沟液中ET水平与TNF-α水平呈显著正相关（P<0.05）。结论 非手术性治疗可降低牙周炎患者龈沟液与血清中ET、VEGF-A、TNF-α水平,改善患者牙周情况。龈沟液中ET水平与TNF-α水平呈显著正相关。     

Graves病合并牙周炎患者IL-6和TNF-α检测及意义

目的: 通过检测Graves病（GD）伴牙周炎(CP)患者龈沟液及血清中白介素6(IL-6)和肿瘤坏死因子α(TNF-α)的浓度,探讨Graves病与牙周炎之间的关系。方法: 采用双免疫酶联法检测30例健康志愿者、30例牙周炎患者、30例Graves病患者、30例Graves病伴牙周炎患者龈沟液及血清中IL-6和TNF-α含量。记录4组研究对象的牙周探诊深度（PD）、临床附着丧失(CAL)和龈沟出血指数（SBI）。采用SPSS 19.0软件包对数据进行统计学处理。结果: GD伴牙周炎组患者血清和龈沟液中IL-6和TNF-α的浓度显著高于单纯牙周炎组及GD组（P<0.05）,GD伴牙周炎组和GD组血清和龈沟液IL-6、TNF-α水平升高与FT3、FT4呈正相关（P<0.05）,且GD伴牙周炎组的相关性显著高于GD组。结论: Graves伴牙周炎组IL-6与TNF-α浓度高于单纯牙周炎组和Graves病组,表明在免疫机制方面,牙周炎与Graves病可能存在相互影响。     

白细胞介素-21在不同牙周状态龈沟液中的表达及相关性分析

目的检测白细胞介素(Interleukin,IL)-21在不同牙周状态下龈沟液中的表达,探讨IL-21在牙周免疫中的可能作用。方法本研究共纳入47例患者,分为健康对照组(15例),轻度牙周炎组(10例),中重度牙周炎组(22例);记录患者一般信息、用Florida牙周探针检查牙周袋探诊深度(PD)和临床附着丧失(CAL)情况;收集牙周治疗前的龈沟液样本,采用酶联免疫吸附法(ELISA)测定不同牙周条件下龈沟液中IL-21的水平;并进行Pearson秩相关检验统计IL-21的表达与相关临床指标的关系。结果 3组龈沟液中IL-21平均浓度分别为(107.20±2.54)、(218.90±5.11)、(367.80±7.27)pg/m L,轻度牙周炎组及中重度牙周炎组龈沟液中IL-21水平显著高于对照组(P<0.05),其中以中、重度牙周炎组增高最为显著;中、重度牙周炎组龈沟液中IL-21水平分别与PD、CAL呈正相关关系(P<0.001)。结论 IL-21在牙周疾病中有较高的表达,尤其是以中重度牙周炎患者最为显著;由此推测IL-21在牙周疾病的发生发展中占有重要地位,与牙周组织的破坏有密切关系。     

慢性牙周炎患者牙周治疗前后龈沟液中白介素-10水平的变化

目的探讨慢性牙周炎患者牙周治疗前后龈沟液中抗炎性细胞因子白介素(IL)-10水平的变化。方法采集12例慢性牙周炎患者的12个健康牙位和36个炎症牙位于治疗前及治疗后6、122、4周的龈沟液,用酶联免疫吸附分析法(ELISA)检测龈沟液中IL-10的浓度。另外,分别记录治疗前、后的探诊深度(PD)、临床附着丧失(CAL)、牙龈指数(GI)和菌斑指数(PlI)。结果IL-10浓度在健康牙位明显高于炎症牙位(P<0.01),且于牙周治疗后明显升高。IL-10浓度与探诊深度(PD)、临床附着丧失(CAL)呈负相关(P<0.05)。结论IL-10浓度与牙周组织破坏程度呈负相关,在牙周炎中起抗炎作用。     

慢性牙周炎龈沟液中硫离子水平与临床相关性研究

目的:分析慢性牙周炎(CP)患者龈沟液中硫离子(su lfides)水平的变化与临床牙周指数的相关关系及其对诊断预后的意义。方法:采用金刚牙周诊断仪进行龈沟液硫离子和牙周临床指标测定。选定实验组(T):36例慢性牙周炎患者,57颗牙位,共342个位点。其中健康牙位(T1)21颗,位点126个;炎症牙位(T2)36颗,位点216。对照组(C):全身及牙周健康者8例,16颗牙位,共96个位点。测定所选位点龈沟液(GCF)中硫化物水平(su lcussu lph ide level,SUL),牙周袋探诊深度(prob ing depth,PD),牙周临床附着丧失水平(c lin ical attachm ent level,CAL),龈沟出血指数(su lcus b leed ing index,SB I)。所有统计结果均采用SPSS11.0进行统计学分析。结果:1)牙周健康对照组(C)GCF中硫离子SUL的浓度均值为(0.0648±0.0169)pg/mL,明显低于慢性牙周炎炎症牙位组(T2)(0.3249±0.0489)pg/mL及慢性牙周炎健康牙位组(T1)(0.1160±0.0271)pg/mL;慢性牙周炎炎症牙位组(T2)GCF中硫离子(SUL)的浓度均值均高于正常对照组及慢性牙周炎健康牙位组(T1)。2)经相关性分析,慢性牙周炎炎症牙位组(T1)GCF中SUL的浓度均值与PD、SB I和CAL均呈正相关关系。而慢性牙周炎健康牙位组(T1)及正常对照组(C)GCF中SUL的浓度均值与PD、SB I及CAL间无相关性。结论:慢性牙周炎(CP)炎症牙位组龈沟液中硫离子(SUL)的浓度均值与牙周临床指标之间具有相关关系,其水平的高低变化可客观反映牙周组织的炎症状态。     

IL-35在牙周炎及口腔扁平苔藓中的表达及相关性分析

目的:探讨IL-35在牙周炎及口腔扁平苔藓免疫机制中的作用及相关影响,以及牙周炎与口腔扁平苔藓之间的相互关系.方法:按纳入标准选择单纯口腔扁平苔藓患者20例,单纯牙周炎患者20例,牙周炎伴口腔扁平苔藓患者20例和健康者12例;记录患者的一般信息及牙周探诊深度、临床附着丧失、牙龈指数、龈沟出血指数,并收集静脉血清及龈沟液样本,采用酶联免疫吸附测定血清及龈沟液中IL-35的表达水平.采用SPSS19.0软件包对数据进行统计学分析.结果:单纯口腔扁平苔藓组、单纯牙周炎组、牙周炎伴口腔扁平苔藓组龈沟液及血清中IL-35浓度显著高于健康对照组(P＜0.01),牙周炎伴口腔扁平苔藓组龈沟液及血清中IL-35浓度显著高于单纯口腔扁平苔藓组及牙周炎组(P＜0.05);各组牙周临床指标与龈沟液及血清中IL-35表达水平呈正相关关系.结论:牙周炎及口腔扁平苔藓中IL-35表达水平显著升高,其升高程度与两者呈正相关.     

侵袭性牙周炎龈沟液中硫化物的检测

目的:检测侵袭性牙周炎(AgP)龈沟液中硫化物浓度水平变化并探讨其与牙周临床指标的关系。方法:采用金刚牙周诊断仪进行龈沟液硫化物和牙周临床指标测定。选定实验组(T)16例侵袭性牙周炎病人,56个牙,共336个位点。其中健康牙(T1)16个,位点96个;炎症牙(T2)40个,位点240。对照组(C):全身、牙周健康者10例,20个牙,共120个位点。测定所选位点龈沟液(gingival crevicu lar flu id,GCF)中硫化物水平(su lcus su lph ide level,SUL),牙周袋探诊深度(pocket prob ing depth,PPD),牙周临床附着丧失水平(c lin i-cal attachm ent level,CAL),龈沟出血指数(su lcus b leed ing index,SB I)。结果:①3组受检牙龈沟液中硫化物浓度不同。侵袭性牙周炎炎症牙位组GCF中硫化物浓度(0.2210±0.0415)×10-6mol/L高于健康牙位组(P<0.05)、正常对照组;健康牙位组GCF中硫化物浓度(0.1025±0.0198)×10-6mol/L高于正常对照组(0.0523±0.0044)×10-6mol/L。②经相关性分析,侵袭性牙周炎炎症牙位组(T1)GCF中硫化物的浓度均值与PD、SB I和CAL均呈正相关关系,健康牙位组(T1)、正常对照组(C)GCF中硫化物浓度均值与PD、SB I及CAL无相关性。结论:侵袭性牙周炎病人龈沟液中的硫化物是参与牙周炎症反应的重要调节因子,其水平变化可反映牙周组织的炎症状态。     

口腔种植修复术在牙列缺损患者中的应用效果及对龈沟液中TNF-α、IL-6水平的影响

目的 探讨口腔种植修复术对牙列缺损患者的治疗效果及对龈沟液中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、白细胞介素6(interleukin-6,IL-6)水平的影响。方法 选择2017年5月—2019年8月上海市浦东新区人民医院口腔科收治的84例牙列缺损患者,随机分为对照组(n=42)和实验组(n=42)。对照组采用常规修复方法,实验组采用口腔种植修复术治疗。治疗后6个月对疗效进行评估,比较2组患者龈沟液中TNF-α、IL-6水平,种植牙功能及并发症发生率。采用SPSS 18.0软件包对数据进行统计学分析。结果 实验组与对照组治疗后6个月TNF-α、IL-6水平显著高于治疗前(P<0.05);实验组治疗后6个月TNF-α、IL-6水平显著低于对照组(P<0.05);实验组与对照组治疗后6个月种植牙功能各项评分均显著高于治疗前(P<0.05);实验组治疗后6个月固位功能、语言功能、咀嚼功能及美观功能评分均显著高于对照组(P<0.05);实验组治疗过程中感染、刺痛感、核桩脱落、牙缺失发生率显著低于对照组(P<0.05)。结论 口腔种植修复术对牙列缺损患者龈沟液中的TNF-α、IL-6水平影响较小,种植牙功能提高,术后并发症发生率降低,值得临床推广应用。     

Er∶YAG激光治疗Ⅱ度根分叉病变牙周炎患者的临床疗效评价

目的 观察并评价Er:YAG（Erbium-doped yttrium aluminium garnet lase, 掺铒钇铝石榴石）激光治疗Ⅱ度根分叉病变牙周炎患者的临床疗效。方法 纳入30例慢性牙周炎出现Ⅱ度根分叉病变患者（60颗患牙）,采取左右半口对照研究。以超声龈上洁治1周后作为基线,随机分为实验组：超声波及手用器械行龈下刮治、根面平整+Er:YAG激光牙周袋内照射,对照组：单纯使用超声波及手用器械行龈下刮治、根面平整治疗对侧同名牙。治疗后12周、20周后比较2组牙龈指数（gingival index, GI）、牙周袋深度（pocket depth, PD）、水平探诊深度 (horizontal probing depth, HPD)、附着丧失 (attachment loss, AL)的变化。采用 SPSS 20.0软件包进行统计学处理。结果 实验组和对照组各牙周临床指标（GI、PD、HPD、AL）在治疗后12周、20周与基线时相比,均显著下降（P<0.05）。治疗后12周和20周,实验组PD分别为 (4.03±0.48) 和 (3.43±0.45) mm,对照组为 (4.82±0.55) 和 (4.27±0.36) mm。经配对样本t检验,实验组PD减小量显著高于对照组（P<0.05）。治疗后12周,2组间HPD无显著差异;术后20周复查HPD发现,实验组为 （3.01±0.34） mm,对照组为（3.78±0.29） mm,实验组HPD降低但仍显著大于对照组（P<0.05）。12周和20周时,实验组GI和AL值较对照组低,但差异无统计学意义。结论 Er:YAG激光对慢性牙周炎Ⅱ度根分叉患者的治疗安全有效,临床应用价值显著。     

维族绝经妇女龈沟液IL-6、血清雌二醇水平与牙周炎的关系

目的:探讨维吾尔族绝经早期的牙周炎患者龈沟液IL-6及血清雌二醇(E2)水平与牙周炎的关系。方法:共79例绝经年限均≤5a的妇女纳入本研究。采集30颗牙周健康牙和49颗牙周炎患牙的龈沟液(GCF),记录牙周临床观察指标。采集慢性牙周炎患者的血样本。应用放射免疫分析法检测GCF中IL-6和血清中E2的浓度。结果:牙周健康组GCF中IL-6浓度为(1088.10±102.33)pg/ml;慢性牙周炎组GCF中IL-6浓度明显高于牙周健康牙组(P<0.005);慢性牙周炎患牙GCF中IL-6浓度与GI、PPD、CAL均呈正相关(r=0.564,P<0.005;r=0.335,P<0.05;r=0.324,P<0.05)。血清E2≤30pg/ml组的妇女牙周炎患牙牙周临床指标、GCFIL-6浓度与E2>30pg/ml组无显著差异(P>0.05)。结论:健康牙GCF中含有微量IL-6。牙周炎患牙GCF中IL-6的水平反映了牙周炎症的严重程度,可以尝试作为判断维族妇女牙周病变程度的一个指标。绝经早期患慢性牙周炎的维族妇女,血清E2水平与牙周炎患牙的病变程度及GCF中IL-6的水平无关。     

牙周炎患者龈沟液中IL—8的含量测定

目的：研究IL－8在牙周炎病程中的变化及与临床指标的关系,方法：采用双抗夹心ABC－ELISA法测定慢性牙周炎（CP）患者,健康对照者以及CP治疗前后患者的龈沟液中IL－8含量,IL－8总量,同时检测临床指标并作相关性检验。结果：CP患者龈沟液中IL－8检出率显著高于健康对照者（P＜0．025）,在CP患者和健康者之间以及CP治疗前后患者的龈沟液中IL－8总量,龈沟液（GCF）量均存在统计学差异（分别为P＜0．01,P＜0．05）,而IL－8含量无统计学差异（P＞0．05）,IL－8总量,GCF量与临床指标存在正相关性（P＜0．01）,结论：IL－8总量在牙周炎病程中呈动态性改变,检测GCF中IL－8的水平对评价牙周炎的程度及指导临床治疗有一定价值。     

Soluble interleukin-1 receptor type II levels in gingival crevicular fluid in aggressive and chronic periodontitis

Background: Interleukin (IL)-1 is closely related to the initiation and progression of periodontal disease. IL-1 levels in the gingival crevicular fluid (GCF) of subjects with periodontitis are higher than those in periodontally healthy controls, and the levels of IL-1 correlate with disease severity. However, soluble IL-1 receptor type II (sIL-1RII), which acts as a decoy receptor for IL-1s, has not been investigated in detail in periodontal disease. The purpose of this study was to measure sIL-1RII levels in the GCF of subjects with chronic or aggressive periodontitis; the correlation between the sIL-1RII levels in GCF and clinical parameters also was examined. Methods: IL-1beta and sIL-1RII were measured in 64 GCF samples collected from 47 subjects with chronic periodontitis (CP) and 17 subjects with aggressive periodontitis (AgP). The clinical characteristics of each site were recorded at the time of GCF sampling. IL-1beta and sIL-1RII were measured by specific non-cross-reactive enzyme-linked immunosorbent assay. Results: The disease severity was comparable in CP and AgP. IL-1beta was detected in 98% of CP GCF samples and 88% of AgP GCF samples. sIL-1RII was detected in 55% of CP GCF samples and 35% of AgP GCF samples. However, the concentrations of IL-beta and sIL-1RII detected in GCF from subjects with CP or AgP were similar. Conclusion: sIL-1RII was detected more often in CP GCF than in AgP GCF, and there was no correlation between GCF sIL-1RII concentration and clinical parameters.     

慢性牙周炎患者龈沟液中白细胞介素-4的检测和意义

目的检测慢性牙周炎患者牙周基础治疗前后龈沟液中白细胞介素-4(IL-4)的质量浓度,探讨IL-4与牙周炎的关系及其在牙周炎发病机制、病情进展等方面所起的作用。方法用滤纸条浸润法采集成年健康者和牙周炎患者治疗前后的龈沟液样本,用酶联免疫吸附测定检测样本中IL-4的质量浓度。结果慢性牙周炎患者龈沟液中IL-4的质量浓度低于健康对照组(P<0.05)。经牙周基础治疗1个月后,IL-4的质量浓度无明显变化,治疗前后的差异无统计意义(P>0.05);IL-4的质量浓度与探诊深度呈显著负相关,与牙龈指数和附着丧失无明显相关性。结论IL-4缺乏可能会导致牙周病的发生,IL-4可作为早期诊断牙周病和检测易患人群的敏感性指标。     

Effect of meloxicam on gingival crevicular fluid IL-1beta and IL1 receptor antagonist levels in subjects with chronic periodontitis, and its effects on clinical parameters

The aim of the present study was to determine the effects of meloxicam after initial periodontal treatment on interleukin-1beta (IL-1β) and IL-1 receptor antagonist (IL-1ra) in gingival crevicular fluid (GCF) and clinical parameters in the chronic periodontitis patients. Data were obtained from 30 patients with chronic periodontitis. Fifteen chronic periodontitis patients received 7.5 mg meloxicam, and 15 patients received placebo tablets in a 1×1 regimen for 1 month. All subjects were nonsmokers and had not received any periodontal therapy. The plaque index (PI), gingival index (GI), probing depth (PD), and clinical attachment level (CAL) were recorded. The GCF was collected using a paper strip: eluted and enzyme-linked immunoabsorbent assays (ELISAs) were performed to determine the cytokine levels. The clinical data and GCF samples were obtained after periodontal therapy and 1 month after periodontal therapy. The PI, GI, PD, and GCF IL-1ra decreased significantly (p<0.05) in meloxicam group at first month when comparing the initial levels. While decrease of the PI was statistically significant in control group (p<0.05), statistically significant changes were not determined in the other clinical parameters and GCF cytokine levels (p>0.05). There were no significant differences between two groups in any of the investigated parameters. Our observations did not reveal any influence of meloxicam on levels of IL-1β and IL-1ra in chronic periodontitis. Additional clinical studies are advisable to determine whether COX-2 selective drugs alter periodontal disease outcome with greater safety.     

Gingival crevicular fluid alkaline phosphatase levels in postmenopausal women: effects of phase I periodontal treatment

BACKGROUND: The aim of this study was to determine how estrogen status may possibly influence gingival crevicular fluid (GCF) alkaline phosphatase (ALP) levels in estrogen-deficient (ED) and -sufficient (ES) postmenopausal women at baseline (BL) and 1 year after periodontal phase I treatment (AT). METHODS: Thirty-six postmenopausal women on estrogen supplements (mean serum estradiol levels >30 pg/ml; estrogen sufficient) and 37 postmenopausal women not on estrogen supplements (mean serum estradiol levels <30 pg/ml; ED) were divided into two subgroups as chronic periodontitis and clinically healthy controls after clinical and radiographic examination. The ES group consisted of 19 control (ES/C) and 17 chronic periodontitis (ES/P) patients, and the ED group consisted of 20 control (ED/C) and 17 chronic periodontitis (ED/P) patients. Plaque (PI) and gingival (GI) indices, bleeding on probing (BOP), probing depths (PD), clinical attachment loss (CAL) scores, and GCF samples were recorded at BL and AT. The levels of ALP in the GCF were measured photometrically. The paired samples Student t and Wilcoxon tests were used to compare the ALP levels and clinical parameters between BL and AT. The correlation among the ALP and clinical parameters was analyzed using the Pearson correlation. RESULTS: The mean of all clinical parameters (PI, GI, BOP, PD, and CAL) was significantly (P <0.05) higher in periodontitis groups (ES/P and ED/P) than controls (ES/C and ED/C). Periodontitis groups (ES/P and ED/P) demonstrated significantly increased GCF volumes and GCF ALP levels (P <0.05) compared to controls (ES/C and ED/C). There were no significant differences in the concentrations of ALP between periodontitis and control groups (P >0.05). The BL GCF ALP total levels of the ED/P group were significantly higher than the ES/P group (P <0.05). The BL and AT serum ALP levels of the ED/P group were not significantly but were numerically higher than the ES/P group. One year after periodontal treatment, the GCF volume, GCF ALP total, and concentrations decreased significantly in both periodontitis groups (P <0.05). However, the GCF ALP levels were still numerically higher in the ED/P group. A positive statistical correlation was found between total ALP levels and PD (r = 0.621; P <0.05). CONCLUSION: These data suggest that the presence of ALP in GCF is not simply a reflection of the local inflammation state and that a patient's estrogen status may possibly influence local ALP levels in GCF.     

Crevicular and serum levels of monocyte chemoattractant protein-4 and high-sensitivity C-reactive protein in periodontal health and disease

ObjectivesChemokines are chemotactic cytokines that are involved in destruction of the periodontal structures. The aim of this study is to determine the presence of MCP-4 and high sensitivity C reactive protein (hsCRP) levels in gingival crevicular fluid (GCF) and serum in periodontal health and disease and to find a correlation between MCP-4 and hsCRP in GCF and serum.Methods40 subjects (20 males and 20 females) were selected and divided into three groups based on clinical parameters and radiologic parameters: Group 1 (10 healthy); Group 2 (15 gingivitis subjects) and Group 3 (15 chronic periodontitis subjects). The levels of serum and GCF MCP-4 were determined by ELISA and hsCRP levels were determined by immunoturbidimetry method.ResultsThe mean GCF and serum concentration of MCP-4 were the highest for group 3 followed by group 2 and least in group 1. Similarly, the mean hsCRP concentrations were highest for group 3 and least in group 1. Moreover, a significant positive correlation was found between serum and GCF MCP-4 and hsCRP levels and periodontal parameters.ConclusionThe levels of MCP-4 and hsCRP increased from healthy to periodontitis. It can be proposed that MCP-4 and hsCRP are the potential biomarkers of inflammation in periodontal health and disease.