非小细胞肺癌组织中VEGF-C和VEGFR-3的表达及其临床意义

背景与目的:血管内皮生长因子-C(vascularendothelialgrowthfactorC,VEGF-C)和VEGFR-3是促进恶性肿瘤淋巴管形成的重要因子,其表达与恶性肿瘤的淋巴结转移关系密切。本文旨在研究VEGF-C和VEGFR-3蛋白在非小细胞肺癌(non-smallcelllungcancer,NSCLC)组织中的表达及其临床意义。方法:应用免疫组化方法检测77例NSCLC组织中VEGF-C和VEGFR-3表达情况,分析其与肿瘤淋巴管密度(lymphaticvesseldensity,LVD)、肿瘤的大小、癌的组织类型、组织分化程度、淋巴结转移情况、临床复发和术后生存期的关系。结果:77例NSCLC组织中有45例(58%)VEGF-C阳性,32例(42%)VEGFR-3阳性。NSCLC组织中VEGF-C表达与肿瘤组织的分化程度有关(r=-0.32,P=0.018);VEGF-C及VEGFR-3表达与肿瘤的淋巴结转移、LVD、肿瘤大小及术后生存期有关。NSCLC组织中VEGF-C与VEGFR-3表达相关(r=0.23,P=0.045)。结论:VEGF-C和VEGFR-3表达与NSCLC的淋巴结转移、预后相关,它的高表达提示肺癌患者容易出现淋巴结转移和预后不良。     

The effect of the expression of vascular endothelial growth factor (VEGF)-C and VEGF receptor-3 on the clinical outcome in patients with gastric carcinoma

Aims

We aimed to investigate the relationship among VEGF-C/VEGFR-3 expression, lymphatic metastasis and patient prognosis in gastric carcinoma.

Material and methods

VEGF-C and VEGFR-3 expression in gastric carcinoma tissues obtained from 204 patients who underwent curative gastrectomy (105 cases presented with lymph node metastasis and 99 cases without metastasis) was examined immunohistochemically. There was no significant difference in the other clinicopathologic variables except for postoperative pathological tumor stage (pT) and TNM stage between the two groups. The results were statistically processed.

Results

The results showed that VEGF-C was located mainly in the cytoplasm of tumor cells and VEGFR-3 was found predominantly in the endothelium of lymphatic vessels. VEGF-C and VEGFR-3 expression was more frequent in gastric carcinoma tissues than that in normal gastric tissues, 54.90% and 35.29% respectively, which revealed that the expression of VEGF-C and VEGFR-3 was significantly stronger in patients with lymph node metastasis than in those without metastasis. Patients who had positive staining for VEGF-C showed significantly less favorable survival rates compared with patients who had negative staining for VEGF-C. The survival rates of patients who had positive staining for VEGFR-3 also were significantly lower compared with patients who had negative staining for VEGFR-3. Patients who had positive staining for both VEGF-C and VEGFR-3 exhibited the most unfavorable prognosis. Multivariate analysis demonstrated that the expression of VEGF-C and VEGFR-3 was an independent prognostic determinant. In addition, faint to moderate VEGF-C expression was detected in normal gastric epithelial cells (18/204, 8.9%).

Conclusions

VEGF-C and VEGFR-3 expression could serve as a prognostic biomarker in patients with gastric carcinoma.     

Prognostic significance of vascular endothelial cell growth factors -A, -C and -D in breast cancer and their relationship with angio- and lymphangiogenesis

Vascular endothelial cell growth factors (VEGF)-A, -C and -D have potent angio and lymphangiogenic functions in experimental models, although their role in the progression of human breast cancer is unclear. The aims of the current study were to examine the relationship between the expression of the aforementioned growth factors with the angio and lymphangiogenic characteristics of breast cancer, and to assess their suitability as potential prognostic factors. Paraffin-embedded sections of 177 primary invasive breast cancer, with complete clinical follow-up information for 10 years, were stained for VEGF-A, -C, -D, podoplanin and CD34 using standard immunohistochemical approaches. The expression of the growth factors was correlated with clinicopathological criteria and patients' survival. Lymph vessel density (LVD) and microvessel density (MVD) were assessed and correlated with expression of the growth factors. Vascular endothelial cell growth factor-A, -C and -D were highly expressed in 40, 37 and 42% of specimens, respectively. High expression of VEGF-A and - C, but not of -D, was associated with a higher LVD (P=0.013 and P=0.014, respectively), a higher MVD (P<0.001 and P=0.002, respectively), the presence of lymph node metastasis (P<0.001 and P<0.001, respectively), distant metastasis (P=0.010 and P=0.008, respectively) and a shorter Overall Survival (P=0.029 and 0.028, respectively). In conclusion, breast cancers that express high levels of VEGF-A and -C are characterised by a poor prognosis, likely through the induction of angio and lymphangiogenesis. Examination of expression of VEGF-A and -C in breast cancer may be beneficial in the identification of a subset of tumours that have a higher probability of recurrence and metastatic spread.     

Vascular endothelial growth factor-D is an independent prognostic factor in epithelial ovarian carcinoma

We assessed the presence of vascular endothelial growth factor (VEGF)-C, VEGF-D and their receptor VEGFR-3 by immunohistochemistry in 59 epithelial ovarian carcinomas, 11 borderline tumours and 20 benign cystadenomas. VEGF-C and VEGF-D were generally expressed in tumour cells and also in endothelia adjacent to tumour nests which showed a strong staining for them. VEGFR-3 was expressed in lymphatic and vascular endothelial cells adjacent to tumour nests. Immunoreactivity was significantly more frequent as lesions progressed from a benign tumour to advanced carcinoma. A strong correlation was found between VEGF-C and VEGF-D detected in carcinoma and VEGFR-3 detected in neighbouring endothelial cells. Increased expression of VEGF-C, VEGF-D and VEGFR-3 was significantly associated with lymph node metastasis and peritoneal metastasis outside the pelvis. There was a significant correlation between the high levels of VEGF-C and VEGF-D proteins, and poor survival. The presence of VEGF-D was an independent prognostic indicator by multivariate analysis. We conclude that VEGF-C, VEGF-D and VEGFR-3 play an important role in lymphatic spread and intraperitoneal tumour development in ovarian carcinoma. Since VEGF-D was found to be an independent predictor of poor outcome, its measurement, together with other prognostic markers may improve prospective identification of patients with a poor prognosis.     

乳腺浸润性导管癌血管内皮生长因子和环氧化酶-2的表达及意义

目的:探讨血管内皮生长因子(VEGF)和环氧化酶-2(COX-2)在乳腺浸润性导管癌组织中的表达及其临床意义。方法:应用免疫组化法检测了100例乳腺浸润性导管癌和40例良性乳腺肿瘤组织中COX-2、VEGF的表达情况,并分析它们与生物学行为、预后的关系。结果:乳腺癌组COX-2和VEGF阳性率分别达54%和66%,与对照组比较差异有统计学意义(P<0·01);COX-2表达与VEGF显著相关(P<0·05)。两者均与乳腺癌预后有关。结论:COX-2、VEGF在乳腺癌组织中的表达较高,是影响乳腺癌患者预后的主要因素之一。因此,联合检测COX-2和VEGF对乳腺癌患者判断预后、指导用药具有重要意义。     

血管内皮生长因子C、D在鼻咽癌组织中的表达及其临床意义

背景与目的:血管内皮生长因子C(vascular endothelial growth factor-C,VEGF-C)和D(vascular endothelial growth factor-D,VEGF-D)是目前已鉴定出的淋巴管生长因子,有研究表明肿瘤组织中VECF-C或VEGF-D过表达与淋巴转移有关.本研究旨在探讨鼻咽癌组织中VEGF-C和VEGF-D的表达情况及其临床意义.方法:采用免疫组化SP法检测66例鼻咽癌组织中VEGF-C和VEGF-D的表达情况,同时检测血管内皮生长因子受体3(vascular endotheliaI growth factor receptor-3,VEGFR-3)和CD34染色情况并计数微淋巴管密度(lymphatic microvessel density,LMVD)和微血管密度(microvessel density,MVD).结果:VEGF-C高表达率在鼻咽癌组织中(54.5%)较鼻咽非肿瘤组织中(26.3%)高(P＜0.05);鼻咽癌伴区域淋巴结转移或T分期晚者,VEGF-C高表达率增高,单因素及多因素Logistic回归分析均表明区域淋巴结转移与VEGF-C高表达相关(P＜0.05),但VEGF-C高表达与性别、年龄、5年生存率、LMVD、MVD等因素无关(P＞0.05).VEGF-D阳性表达率在鼻咽癌组织中(69.7%)较鼻咽非肿瘤组织中(42.1%)高(P＜0.05);鼻咽癌中VEGF-D阳性表达与性别、年龄、T分期、区域淋巴结转移、LMVD、MVD等因素无关(P＞0.05),但与VEGF-C高表达显著正相关(P＜0.01),VEGF-D阳性表达者5年生存率(50.0%)显著低于VEGF-D不表达者(85.0%)(P＜0.01).结论:鼻咽癌中VEGF-C高表达与区域淋巴结转移密切相关;VEGF-D阳性表达与区域淋巴结转移无关,但与VEGF-C高表达正相关,且与5年生存率密切相关.     

Expression of vascular endothelial growth factor (VEGF)-D and its receptor, VEGF receptor 3, as a prognostic factor in endometrial carcinoma.

PURPOSE: To evaluate the prognostic value of vascular endothelial growth factor (VEGF)-D and VEGF receptor (VEGFR)-3 in endometrial carcinoma. EXPERIMENTAL DESIGN: We assessed the levels of immunoreactivity for VEGF-D and VEGFR-3 in 71 endometrial carcinomas, 14 complex atypical endometrial hyperplasias, and 16 normal endometria by immunohistochemistry. RESULTS: VEGF-D was stained in both tumor cells and adjacent stromal cells. VEGFR-3 was stained in both tumor cells and adjacent endothelial cells. Immunoreactivity for VEGF-D in tumor cells and adjacent stromal cells became significantly stronger as lesions progressed from normal endometrium to advanced carcinoma. Similarly, immunoreactivity for VEGFR-3 in tumor cells and adjacent endothelial cells was significantly greater as lesions progressed from normal endometrium to advanced carcinoma. A strong correlation was found between high levels of VEGF-D immunoreactivity in carcinoma cells and VEGFR-3 in both carcinoma cells and adjacent endothelial cells. Similarly, high levels of VEGF-D immunoreactivity in stromal cells were significantly correlated with those of VEGFR-3 in both carcinoma cells and endothelial cells. High levels of VEGF-D in carcinoma cells and stromal cells, as well as those of VEGFR-3 in carcinoma cells and endothelial cells, were significantly related to myometrial invasion and lymph node metastasis. A strong correlation was found between poor survival and high levels of VEGF-D in both carcinoma cells and stromal cells and between poor survival and high levels of VEGFR-3 in carcinoma cells. Moreover, the high levels of VEGF-D in stromal cells and VEGFR-3 in carcinoma cells were independent prognostic factors in endometrial carcinoma. CONCLUSIONS: The presence of VEGF-D and VEGFR-3 in endometrial carcinoma may predict myometrial invasion and lymph node metastasis and may prospectively identify patients who are at increased risk for poor outcome. In addition, VEGF-D and VEGFR-3 may be promising targets for new therapeutic strategies in endometrial carcinoma.     

食管鳞癌中血管内皮生长因子－C 的表达及与淋巴管生成的关系

目的：探讨血管内皮生长因子－C（VEGF －C）在食管鳞癌中的表达及其与淋巴管生成、淋巴结转移的关系。方法：收集2013年3月至2014年1月遂宁市中心医院胸心外科的107例食管鳞癌手术切除病例及56例正常食管组织的石蜡包埋组织样本,采用免疫组化检测 VEGF －C 在食管鳞癌中的表达,并应用 D2－40抗体标记组织中的微淋巴管内皮细胞,计数微淋巴管密度（LVD）。同时对其与临床病理参数的关系进行分析。结果：食管鳞癌中 VEGF －C 蛋白的高表达率明显高于正常食管组织,且在食管鳞癌不同 TNM分期中有差异（P ＜0．05）;淋巴结转移组中 VEGF －C 蛋白的高表达率为68．3％,高于无淋巴结转移组（P ＜0．05）。T3／T4期组中 VEGF －C 蛋白的高表达率高于 T1／T2期组（P ＜0．05）。VEGF －C 蛋白的高表达率与食管鳞癌患者的年龄、性别、分化程度、肿瘤大小等均无明显相关性（P ＞0．05）。食管鳞癌中 LVD 在 VEGF －C 不同表达强度组中有差异,差异有统计学意义（P ＜0．05）。食管鳞癌淋巴结转移组 LVD 高于无淋巴结转移组,差异均有统计学意义（P ＜0．05）。结论：VEGF －C 基因可能促进食管鳞癌的淋巴管生成及淋巴结转移,有可能成为预测食管鳞癌预后的实验室指标。     

Clinicopathological significance of vascular endothelial growth factor (VEGF)-C in human esophageal squamous cell carcinomas

The purpose of this study was to investigate the expression of vascular endothelial growth factor (VEGF) -C in human esophageal squamous cell carcinomas to elucidate its role in lymph node metastasis and tumor progression. The expression of VEGF-C and flt-4 genes was examined in 5 esophageal carcinoma cell lines, 12 fresh biopsy specimens and 48 archival surgical specimens of human esophageal carcinoma tissues by RT-PCR and immunohistochemistry. Immunohistochemistry using antibodies against CD34 (endothelial cell specific) was also carried out and microvessels were quantified by counting vessels in a 200x field in the most vascular area of the tumor. Of the 5 human esophageal carcinoma cell lines, 4 constitutively expressed VEGF-C mRNA. In 8 (66.7%) of 12 cases, VEGF-C mRNA was detected in only tumor tissues but not in normal mucosa by RT-PCR. There was a significant relationship between VEGF-C and flt-4 mRNA expression. Out of the 48 surgical specimens of esophageal carcinomas, 19 (39.6%) and 10 (20.8%) exhibited intense VEGF-C immunoreactivity in the cytoplasm of many cancer cells and the stromal cells, respectively. In contrast, Flt-4 was mainly expressed on the lymphatic endothelial cells. Normal and dysplastic esophageal squamous epithelium exhibited no or faint cytoplasmic staining of VEGF-C. VEGF-C expression correlated with depth of tumor invasion, tumor stage, venous invasion, lymphatic invasion and lymph node metastasis. Vessel count was significantly higher in the VEGF-C positive tumors than in the negative tumors. These results overall suggest that VEGF-C may play a role in tumor progression via lymphangiogenesis and angiogenesis in human esophageal carcinoma.     

VEGF、VEGF-C、Flt-4蛋白和MVD、LVD在乳腺癌组织中的表达及临床意义

目的探讨乳腺癌组织中VEGF、VEGF-C、Flt-4蛋白表达水平、MVD、Flt-4阳性脉管数及LVD六项指标之间的相互关系及其临床意义。方法采用免疫组织化学S-P法,检测105例乳腺癌石蜡标本中VEGF、VEGF-C、Flt-4、MVD、LVD及Flt-4阳性脉管数的表达水平。结果VEGF表达水平与MVD有显著相关性(P<0.01);VEGF-C表达水平与Flt-4表达水平有显著相关性(P<0.01),且二者均与Flt-4阳性脉管数及LVD有显著相关性(P<0.01)。在淋巴结转移组中,VEGF-C蛋白表达水平、Flt-4阳性脉管数及LVD均显著高于无淋巴结转移组(P<0.05)。在腋淋巴结阴性的血行转移患者中,VEGF及MVD的表达显著高于腋淋巴结阳性的血行转移患者(P<0.05),而VEGF-C、Flt-4及LVD的表达却显著低于后者(P<0.05)。MVD及VEGF-C的阳性表达与乳腺癌血行转移危险密切相关。结论VEGF促进微血管生成;VEGF-C、Flt-4促进淋巴管生成,且二者有显著相关性(P<0.01);VEGF-C、Flt-4及LVD与淋巴结转移状况密切相关;VEGF及MVD与腋淋巴结阴性患者血行转移相关;MVD及VEGF-C可能是乳腺癌患者发生血行转移的危险因素。     

Expression of Vascular Endothelial Growth Factor-C and Vascular Endothelial Growth Factor Receptor-3 in Ovarian Epithelial Tumors

Objective： To explore the role of vascular endothelial growth factor-C （VEGF-C） in the process of angiogenesis, lymphangiogenesis and lymphatic metastasis in epithelial ovarian tumors. Methods： In situ hybridization and immunohistochemical staining for VEGF-C were performed in 30 epithelial ovarian carcinomas, 9 borderline tumors and 26 benign tumors. Endothelial cells were immunostained with anti-VEGFR-3 pAb and anti-CD31 mAb, and VEGFR-3 positive vessels and microvessel density （MVD） were assessed by image analysis. Results： VEGF-C mRNA and protein expression were detected in cytoplasm of carcinoma cells. VEGF-C mRNA and protein expression in ovarian epithelial carcinomas were significantly higher than those in borderline tumors and benign tumors （P〈0.05 or P〈0.01）. In ovarian epithelial carcinomas, VEGF-C protein expression, VEGFR-3 positive vessels and MVD were significantly higher in the cases of clinical stage Ⅲ-Ⅳ and with lymph node metastasis than those of clinical stage Ⅰ-Ⅱ and without lymph node metastasis respectively （P〈0.05 or P〈0.01）. VEGFR-3 positive vessels and MVD were significantly higher in VEGF-C protein positive tumors than negative tumors （P〈0.05）. VEGFR-3 positive vessels was significantly correlated with MVD（P〈0.01）. Conclusion： VEGF-C might play a role in lymphatic metastasis via lymphangiogenesis and angiogenesis in epithelial ovarian tumors, and VBEGF-C could be used as a biologic marker of metastasis in ovarian epithelial tumors.     

ER/PR阴性乳腺癌的临床病理特征及预后

目的 探讨非激素依赖性乳腺癌的临床病理特征及预后相关因素.方法 收集213例1999年1月至2006年12月术前未接受任何治疗的ER、PR阴性乳腺癌患者的临床、病理资料,随访追踪患者生存信息.应用组织微阵列技术、免疫组织化学染色及免疫荧光技术,检测HER2、CK5/6、CK14、EGFR、p53、p63、Vimenti...     

VEGF-C 在乳腺癌组织中的表达及意义

目的：探讨血管内皮生长因子-C（VEGF—C）在乳腺癌组织中的表达及意义。方法：应用Elivision二步免疫组化法，观察45例乳腺癌和7例乳腺良性增生性病变组织中VEGF—C的表达情况、ER和PR、FⅧ阳性的血管密度，分析其与临床病理指标之间的关系。结果：45例乳腺癌组织中有25例VEGF—C表达阳性，阳性率为55．6％，而7例乳腺良性病变中有4例阳性，阳性率为57．1％，两者之间无显著差异（P=1．00）；45例乳腺癌组织中VEGF—C的表达与患者年龄、肿瘤大小、淋巴结转移及临床分期之间均无统计学相关性（P均大于0．05）；VEGF—C的表达与MVD之间呈显著正相关（r=0．325，P=0．030）；VEGF—C的表达与ER（P=0．248）及PR（P=0．071）的表达之间无显著性相关。结论：VEGF—C与MVD呈显著正相关，提示其有促进血管生成的作用。VEGF—C的表达与激素受体之间无显著相关性，其表达可能不受激素的调控。     

血管内皮生长因子C在人乳腺癌细胞株MCF-7及MCF-7/Adr中的表达

背景与目的：血管内皮生长因子C（vascular endothelial growth factor C,VEGF-C)是近期发现的血管内皮生长因子（vascular endothelial growth factor,VEGF)家族的新成员。研究发现VEGF－C可特异性作用于淋巴管内皮细胞,并在多种人类肿瘤中表达。为探索VEGF－C在肿瘤发生,发展中的作用,我们分别检测了VEGF－C mRNA蛋白在人乳腺癌细胞株MCF－7及其耐药株MCF－7／Adr中的表达。方法;根据VEGF－C基因序列,设计合成地高辛标记的特异性寡核 探针,运用原位杂交方法检测培养的细胞株MCF－7和MCF－7／Adrk VEGF-C mRNA的表达,并运用免疫组织化学方法检测两种细胞中VEGF－C蛋白的表达,实验中用缓冲液分别代替杂交液和一抗作阴性对照。结果：原位杂交法检测到MCF－7和MCF－7／Adr细胞的胞浆中有阳性蓝色颗粒,免疫组化检测发现两种细胞的胞浆中均有阳性棕黄色颗粒,而阴性对照细胞的胞浆中则均无阳性颗粒。结论：人乳腺癌细胞株MCF－7及其耐药株MCF－7／Adr细胞能够转录VEGF-C mRNA,并在其细胞浆中翻译合成相应的蛋白。     

VEGF-C和VEGF-R3在非小细胞肺癌中的表达及其意义

目的　探讨VEGF C及其受体VEGF R3与非小细胞肺癌患者临床病理特征之间的关系。方法　采用免疫组化方法 ,检测 84例非小细胞肺癌组织中VEGF C及VEGF R3表达 ,将表达结果与临床病理特征及预后进行统计学分析。结果　VEGF C和VEGF R3在肺癌细胞膜和细胞浆中高表达 (阳性率分别为5 5 .9%和 5 9.5 % ) ,而在癌旁肺组织中无表达。VEGF C表达与病理类型有密切关系 (P =0 .0 13 ) ;VEGF R3表达与淋巴结转移状况 (P =0 .0 0 2 )及TNM分期 (P =0 .0 2 0 )有密切关系。多因素分析表明 ,VEGF R3 (P <0 .0 0 1)和病理类型 (P =0 .0 2 0 )是影响预后的独立危险因素。结论　检测肺癌组织中VEGF R3表达有助于预测非小细胞肺癌患者预后。     

Clinicopathological significance of tissue homeostasis in Indian breast cancer

BACKGROUND: Tissue homeostasis and the maintenance of cell populations depend on a delicate balance between the rates of cell proliferation and cell death. Disruption of this balance is an important factor in development and progression of tumors. In the present study we evaluated the growth index in a large group of breast cancer patients and correlated it with various clinical and histopathological features of the tumor. METHODS: Estimation of apoptosis was determined by TUNEL assay while immunocytochemistry for proliferating nuclear cell antigen (PCNA) was used as a measure of proliferation. Necrosis was identified morphologically by haematoxylin and eosin staining. RESULTS: A positive correlation was observed between the percentage of PCNA positive cells and the frequency of mitosis (r=0.6117, p<0.0001). A highly statistical significant correlation was observed between type of tissue analyzed and growth index (r=0.46869, p<0.0001). No significant association was observed between hormone receptor status and growth index. CONCLUSIONS: The growth index was found to be higher in carcinoma cells that metastasised into lymph nodes compared with primary lesions with no nodal metastasis. Growth index was particularly prominent in high-grade tumors in which increased proliferative activity was evident. Apoptotic cells were detected more frequently in tumor cells with higher rather than lower proliferative activity. This suggests that not only proliferative activity but also capacity for apoptosis is altered in breast tumors.     

乳腺癌中VEGF的表达及其临床意义

 目的　分析乳腺癌中血管内皮生长因子 (VEGF)的表达状况及其对预后的影响。方法　检测5 8例乳腺癌组织切片中VEGF及增殖细胞核抗原 (PCNA)的表达状况 ,统计分析VEGF表达与PCNA、雌激素受体 (ER)表达的关系 ,与乳腺癌组织学分级的关系 ,并结合临床随访资料进行生存分析。结果　VEGF阳性表达 33/ 5 8,组织学分级Ⅰ级与Ⅱ级、Ⅲ级之间VEGF表达有显著性差异 ,P =0 .0 37,VEGF表达阳性组PCNA的表达显著高于阴性组且其生存时间显著低于后者 ;COX模型比例风险回归分析显示 ,VEGF表达是影响乳腺癌预后的独立因素之一。结论　乳腺癌中VEGF的表达显著影响预后 ,是独立的预后因子 .     

乳腺癌组织中VEGF的表达及临床意义

目的 研究血管内皮生长因子(vascular endothelial growth factor,VEGF)在乳腺癌中的表达及临床意义.方法 应用免疫组织化学S-P法检测52例乳腺癌组织和18例乳腺良性病变中VEGF的表达情况,结合临床及病理形态学资料进行统计分析.结果 VEGF正常乳腺组织中阳性表达率低于乳腺癌组织的阳性表达率(P＜0.05).VEGF在腋淋巴结阳性组和复发/远处转移组的阳性率明显高于腋淋巴结阴性组和未发生远处转移/复发组(P＜0.05);乳腺癌VEGF表达与ER(P＜0.05)、PR(P＜0.05)、生存期(P＜0.05)呈负相关;与CerbB-2(P＜0.05)、临床分期(P＜0.05),呈正相关.VEGF与患者年龄、肿块大小无显著性差异(P＞0.05).结论 VEGF可能与乳腺癌的进展、转移及预后有关,作为独立的指标对判定预后和制定治疗方案具有参考意义.     

胃癌组织中血管内皮生长因子的表达对血管和淋巴管生成的影响及其预后意义

目的 研究胃痛组织中血管内皮生成因子(VEGF)-A、VEGF-C和VEGF-D的表达对血管和淋巴管生成的影响及其预后意义.方法 采用免疫组化法检测123例原发性胃癌组织中VEGF-A、VEGF-C和VEGF-D的表达,采用D2-40和CD34免疫组化双染法分别标记淋巴管和血管,并测定淋巴管密度(LVD)和血管密度(MVD).采用单因素分析VEGF-A、VEGF-C和VEGF-D表达与胃癌临床病理特征的关系;采用Kaplan-Meier法和Log rank检验评价VEGF-A、VEGF-C和VEGF-D表达与胃癌患者预后的关系,并用Cox比例风险模型进行多因素分析.结果 123例胃癌组织中,VEGF-A、VEGF-C和VEGF-D的高表达率分别为64.2%、65.9%和41.5%.VEGF-A、VEGF-C或VEGF-D高表达及两两高表达均与LVD有关(均P＜0.05);VEGF-A和VEGF-C高表达还与浸润深度、淋巴管浸润、静脉浸润、淋巴结转移和MVD有关(均P＜0.05);VEGF-C和VEGF-D高表达均与淋巴管浸润和淋巴结转移有关(均P＜0.05).VEGF-A、VEGF-C或VEGF-D高表达者的生存时间均明显短于其低表达者(均P＜0.05),其中VEGF-A和VEGF-C均高表达者的生存时间最短(56个月).VEGF-A的表达水平、MVD、淋巴结转移及浸润深度是影响胃癌患者预后的独立因素.结论 VEGF-A和VEGFC均高表达的胃癌有更强的促进血管和淋巴管生成的能力,并可促进肿瘤转移和影响患者预后;VEGF-C和VEGF-D可共同介导淋巴管生成,促进淋巴结转移;但仅VEGF-A高表达是影响患者预后的独立危险因素.