共查询到20条相似文献,搜索用时 31 毫秒
1.
S. B. Tkachenko V. N. Kokryakov I. P. Ashmarin S. V. Grachev A. A. Kubatiev 《Bulletin of experimental biology and medicine》1994,118(6):1291-1294
The effect of the total fraction of human defensins (HNP-1, HNP-2, and HNP-3) on the cytoplasmic Ca2+ content ([Ca2+]i) in the platelets of healthy donors was studied. At concentrations of 0.1–40 μg/ml and an incubation time of 10 min defensins
have no effect on [Ca2+]i in platelets labeled with Fura-2AM. However, at higher concentrations (100 μg/ml) they increased platelet [Ca2+]i. In addition, defensins (40 μg/ml) inhibited the Ca2+ increase in platelets induced by thrombin, adenosine diphosphate, and the lipopolysaccharide ofS. typhimurium endotoxin. The most pronounced inhibitory effect was observed in a suspension of thrombin-stimulated platelets. It is shown
that the effect of human defensins on the functional activity of platelets is due to the alterations in the intracellular
Ca2+.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
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12, pp. 600–603, December, 1994 相似文献
2.
B. Kh. Yagmurov A. A. Timofeev S. K. Soodaeva A. G. Chuchalin 《Bulletin of experimental biology and medicine》1994,117(6):622-625
The development of bronchospasm is shown to be accompanied by lipid peroxidation (LPO) activation; 3-fold and 8-fold rises
of malondialdehyde concentration are found in homogenate of lung from sensitized animals and from animals provoked with egg
albumin antigen, respectively. The use of luminol-dependent chemiluminescence (CL) reveals that in sensitized rats the production
of oxygen free radicals is increased by alveolar macrophages activated with phorbol myristate acetate. Troventol at 10−3 mg/ml inhibits the CL response of phagocytes both in intact and in sensitized rats and lowers the level of Fe2+-induced LPO in lung tissue but not in the liver of intact animals.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N
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6, pp. 619–621, June, 1994 相似文献
3.
V. M. Smirnov 《Bulletin of experimental biology and medicine》1997,124(1):635-638
The mechanism by which heart rate is increased upon stimulation of vagal centers is studied using frog heart preparations
perfused with Ringer—Locke solution containing atropine and/or benzohexonium. Atropine stimulates vagus-induced heart-rate
acceleration in dilutions of 10−6 and 10−5 g/ml. In a dilution of 10−4 g/ml both atropine and benzohexonium abolish vagal tachycardia. Rausedyl (3–4 injections, 5 mg/kg, at 18–20-h interval) prevents
tachycardia. Stimulation of both halves of the medulla oblongata increases heart rate to a greater extent than stimulation
of one half.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 124, No. 7, pp. 16–20, July, 1997 相似文献
4.
T. V. Grekhova R. R. Gainetdinov T. D. Sotnikova L. M. Krasnykh V. S. Kudrin S. A. Sergeeva I. S. Morozov 《Bulletin of experimental biology and medicine》1995,119(3):294-296
It is demonstrated that bromantane induces a pronounced and prolonged (8 h) increase in the release of dopanime. Tetrodotoxin
(10−6 M) perfused via a microdialysis probe partially inhibits the bromantane-induced release of dopamine. The extracellular content
of the dopamine metabolites 3,4-dioxyphenylacetic and homovanillic acids is slightly decreased. The possible mechanisms of
action of bromantane on the dopamin- and serotoninergic systems of the brain are discussed.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N
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. 3, pp. 302–304, March, 1995
Presented by M. D. Mashkovskii, Member of the Russian Academy of Medical Sciences 相似文献
5.
N. G. Bogdanova T. V. Lelekova N. P. Pal'mina 《Bulletin of experimental biology and medicine》2000,129(1):30-32
Effects of the regulatory peptide thyroliberin on microviscosity of lipid components of endoplasmic reticulum biological membranes
in mouse hepatocytes were studied by electron paramagnetic resonance. Thyroliberin in a concentration of 10−3–10−18 M decreased microviscosity of surface layers of membrane lipids. This decrease was the most pronounced (30%) under effects
of 10−10 and 10−16 M thyroliberin. Physiological effects of thyroliberin corresponded to its influence on the membrane structure.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 129, No. 1, pp. 38–40, January, 2000 相似文献
6.
T. V. Byzova T. N. Vlasik A. V. Mazurov 《Bulletin of experimental biology and medicine》1994,118(4):1102-1105
Monoclonal antibodies CRC64 are obtained against Ca2+-dependent glycoprotein IIb–IIIa complex of the platelet membrane which possess the ability to inhibit completely fibrinogen-dependent
platelet aggregation. CRC64 is directed against the epitope formed by the glycoprotein IIb–IIIa complex and does not interact
with proteins isolated after platelets are treated with ethylenediamine tetraacetate. Complete, reproducible blockade of platelet
aggregation caused by 5 μM adenosine diphosphate is noted in an MCA concentration of 3 μg/ml, while in the case of a stronger
inductor, namely 1 U/ml thrombin, platelet aggregation is inhibited in a concentration of 5 μg/ml. F(ab′)2 fragments are also able to inhibit platelet aggregation completely and are usually effective in concentrations lower than
native monoclonal antibodies.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
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10, pp. 402–405, October, 1994
Presented by V. N. Smirnov, Member of the Russian Academy of Medical Sciences 相似文献
7.
E. I. Solntseva Yu. V. Bukanova R. U. Ostrovskaya T. A. Gudasheva T. A. Voronina V. G. Skrebitskii 《Bulletin of experimental biology and medicine》1996,121(2):138-142
The effect of two nootropics, piracetam and N-phenylacetyl-L-prolyglycine ethyl ester (GVS-111), is studied by measuring high-threshold
K+ and Ca2+ currents in isolated snail neurons using a two-microelectrode patch-clamp technique. Piracetam and GVS-111 are shown to reduce
the amplitude of both the K+ and the Ca2+ (to a lesser extent) current. The threshold concentrations for GVS-111 and piracetam are 10−9-10−8 M and 1–5×10−4 M, respectively. It is assumed that the antiamnestic effect of the nootropics is partially mediated by a blockade of ion
channels of the neuronal membrane.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N
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2, pp. 151–155, February, 1996
Presented by G. N. Kryzhanovskii, Member of the Russian Academy of Medical Sciences 相似文献
8.
Physiological concentrations of endothelin-1 cause only coronary vasodilatation in anesthetized rats
M. A. Grafov N. A. Medvedeva O. S. Medvedev 《Bulletin of experimental biology and medicine》1995,120(1):661-664
Various concentrations of the peptide endothelin-1 were tested for their effect on coronary vascular resistance in anesthetized
rats. Intracoronary infusion of this peptide over 4 min in a total dose that gave rise to blood concentrations of 0.002–0.003
or 0.2–0.3 fmol/ml elicited only a decrease in the estimated coronary vascular resistance. It is suggested that only supraphysiological
doses of endothelin are likely to exert a powerful vasoconstrictive effect on coronary vessels and cause myocardial ischemia.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N
o
7, pp. 16–19, July, 1995
Presented by I. P. Ashmarin. Member of the Russian Academy of Medical Sciences 相似文献
9.
N. F. Avrova V. P. Ivanova V. A. Tyurin I. A. Gamalei I. V. Klyubin I. A. Shchepetkin E. V. Borunov Yu. Yu. Tyurina 《Bulletin of experimental biology and medicine》1994,117(1):45-47
It is shown that ganglioside GM1 in picomolar concentrations stimulates the phorbol-12-myristate-13-acetate (PMA)-induced
generation of active forms of oxygen by neutrophils and peritoneal macrophages. GM1 (10−11 M) is found to enhance the luminol-dependent chemiluminescence induced by 10−8 M PMA in mouse macrophages in comparison with the effect of PMA alone.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N
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1, pp. 44–46, January, 1994
Presented by A. N. Klimov, Member of the Russian Academy of Medical Sciences 相似文献
10.
G. V. Burlakov 《Bulletin of experimental biology and medicine》1994,118(3):949-952
The effect of lectins (phytohemagglutinin — PHA, concanavalin A — ConA,Pisum sativum lectin — PSL,Ricinus communis lectin — RCL, and pokeweed mitogen — PWM) on the cardiac cholinoceptors is studied in experiments on isolated hearts of maleRana temporaria frogs. The test lectins in concentrations from 10−23 to 10−3 are shown to exhibit cholinomimetic properties.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
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9, pp. 252–255, September, 1994
Presented by A. D. Ado, Member of the Russian Academy of Medical Sciences 相似文献
11.
Yu. P. Kashperskii A. A. Adamyan V. A. Makarov S. P. Glyantsev V. A. Zhukov 《Bulletin of experimental biology and medicine》1995,120(1):708-711
The hemostatic properties of ozone were studied on models of parenchymatous (from a wound in the liver) and stem (from the
stump of the tail) bleedings in rats. An air-ozone mixture at a flow rate of 1 liter/min and a concentration of 2 mg/ml was
found to exert a pronounced hemostatic effect. Our findings indicate that the arrest of bleeding under the influence of ozone
is due to the formation of a fibrin membrane on the surface of the flowing blood, this leading to rapid and effective hemostasis.
Preliminary drying of the wound still further speeds the onset of hemostasis.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120 N
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7 pp. 62–65, July, 1995
Presented by D. S. Sarkisov, Member of the Russian Academy of Medical Sciences 相似文献
12.
I. P. Ashmarin V. E. Pastorova L. A. Lyapina 《Bulletin of experimental biology and medicine》1998,125(5):437-439
Elementary proline-containing peptides being added to rat platelet-rich plasma or platelet suspension in concentrations of
10−1–10−7 mg/ml elicit a considerable antiplatelet effect. Efficiency of inhibition of platelet aggregation increases in the following
order: Pro-Gly>Pro-Gly-Pro>Gly-Pro-Gly-Gly. Intravenous injection of these peptides activates blood anticoagulation system
in experimental animals.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 125, No. 4, pp. 496–499, May, 1998 相似文献
13.
Synthesis and properties of the immunotoxin CD5-ricin 总被引:1,自引:0,他引:1
G. V. Vikha L. D. Uzhinova A. Zh. Kashaeva V. V. Goshev A. V. Krylov A. Yu. Baryshnikov R. G. Vasilov 《Bulletin of experimental biology and medicine》1995,119(1):70-73
Synthesis and properties of an immunotoxin produced by conjugating ricin with a novel monoclonal antibody (IgG3 of the ICO-104
class) are described. Cytolytic activity of the synthesized immunotoxin, determined by two independent methods and expressed
in LD50, is 0.3–0.6×10−7 M. Its specificity for target cells containing the CD5 antigen is shown.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N
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1, pp. 76–79, January, 1995
(Presented by Yu. A. Romanov, Member of the Russian Academy of Medical Sciences) 相似文献
14.
M. I. Musatov S. G. Zhabin V. I. Konenkov N. A. Zorin 《Bulletin of experimental biology and medicine》1994,118(3):1006-1008
It is shown that plasmin in doses of 0.1, 1, or 10 μg/ml did not influence significantly phytohemagglutinin-induced proliferation
of mononuclear lymphocytes in a 3-day culture with these cells. Their proliferative response to pokeweed mitogen was stimulated
by plasmin in the dose of 10 μg/ml only. Biogenic complexes of plasmin with α2-macroglobulin or α2-antiplasmin induced a moderate reduction of spontaneous proliferation after 3 days of culture, and so did plasmin after 5
days; α2-macroglobulin induced a dose-dependent comitogenic effect with phytohemagglutinin and pokeweed mitogen, while α2-antiplasmin induced a dose-independent comitogenic effect with pokeweed mitogen.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
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9, pp. 304–305, September, 1994
Presented by V. A. Trufakin, Member of the Russian Academy of Medical Sciences 相似文献
15.
G. A. Belokrylov O. Ya. Popova O. N. Derevnina I. V. Molchanova 《Bulletin of experimental biology and medicine》1994,117(5):497-499
It is shown that the effect of amino-acid preparations (levamine-70–70, cerebrolysin, and aviamine) is dose-dependent. Thus,
levamine-70 and cerebrolysin at 65 mg/kg do not affect the immune response but stimulate phagocytosis. Aviamine at 65 mg/kg
inhibits the immune response but stimulates phagocytosis and in a dose of 6.5×10−2 mg/kg boosts both processes.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N
o
5, pp. 500–501, May, 1994
Presented by A. D. Ado, Member of the Russian Academy of Medical Sciences 相似文献
16.
G. S. Yakobson A. R. Antonov V. V. Letyagina D. G. Sakharov A. L. Markel' 《Bulletin of experimental biology and medicine》1995,120(4):1006-1009
A comparative electrocardiographic evaluation of changes in cardiac function in rats with hereditary arterial hypertension
(NISAG strain) and normotensive (Wistar) rats in response to a single epinephrine injection revealed much more pronounced
changes in NISAG rats, including an unfavorable time course of electrocardiographic waves (left ventricle overload) and impaired
conduction (blockade) and excitability (extrasystoles). The results indicate that the myocardium of NISAG rats is much more
responsive to the acute stimulation of adrenergic receptors by epinephrine than is the myocardium of normotensive rats.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N
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10, pp. 372–375, October, 1995
Presented by V. A. Trufakin, Member of the Russian Academy of Medical Sciences 相似文献
17.
T. K. Dubovaya A. Yu. Tsibulevskii A. P. Ettinger M. D. Polivoda L. B. Pirogova V. M. Kim 《Bulletin of experimental biology and medicine》1994,118(3):1032-1034
Vagotomy is shown to result in disturbances of the microcirculation (a reduced rate of blood flow and distortion of its kinetics),
the morphological basis of which consists of certain transformations of the microvascular network organization and ultrastructural
changes in the cells lining the sinusoidal capillaries. The most pronounced disorders in microhemodynamics and blood supply
of the liver are found 5–14 days after vagotomy.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
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9, pp. 328–330, September, 1994
Presented by V. V. Kupriyanov, Member of the Russian Academy of Medical Sciences 相似文献
18.
I. G. Rebrov G. N. Kryzhanovskii N. P. Belykh T. F. Shukalova R. N. Glebov 《Bulletin of experimental biology and medicine》1994,118(2):852-855
A study is performed of the effect of the convulsants picrotoxin and bicuculline, blockers of GABA-dependent Cl−-conductivity, on the rate of desensitization of muscimol-induced36Cl− entry into synaptoneurosomes isolated from rat cortex. Both picrotoxin and bicuculline, despite the difference in the mechanisms
of inhibition of the GABA receptor/Cl− ionophore complex, markedly reduce the rate of desensitization. However, the initial moment of the action of both convulsants
is characterized by inhibition of Cl− transport alone, without a drop of the rate of desensitization.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
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8, pp. 160–163, August, 1994 相似文献
19.
N. G. Kolosova N. A. Solov'eva I. G. Shabalina R. I. Salganik 《Bulletin of experimental biology and medicine》1996,121(3):259-261
It is shown that at the age of 2–3 months S rats with genetically determined hyperproduction of free radicals have the same
content of tocopherol in the heart, epididymal fat, adrenals, liver, and liver mitochondria as Wistar rats but a lower content
of plasma tocopherol. At 10–12 months, the tocopherol content in all studied tissues and organs, except the liver, is higher
than in young S rats and age-matched Wistar rats. This is regarded as a compensatory response facilitating the stabilization
of LPO under conditions of increased free radical formation.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N
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3, pp. 282–284, March, 1996 相似文献
20.
E. V. Eliseeva N. V. Kulakova V. A. Nevzorova 《Bulletin of experimental biology and medicine》2000,130(2):763-765
Nitric oxide synthase of the bronchial epithelium and concentrations of nitric oxide metabolites (NO2
− and NO3
−) in bronchoalveolar lavage fluids were measured in rats with bronchial asthma after fenoterol inhalation. It was suggested
that nitric oxide-ergic mechanisms can mediate the effects of inhaled β2-adrenergic agonists.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 130, No. 8, pp. 176–179, August, 2000 相似文献