两种流式单平台计数法测定移植术后患者外周血T淋巴细胞亚群的应用评价

目的评价基于流量传感器的单平台计数法(简称体积法)与基于Trucount管的单平台计数法(简称Trucount法)对移植术后患者外周血T淋巴细胞计数的应用价值。方法分别用Trucount法和体积法检测107例肝/肾移植术后患者外周血CD4~+、CD8~+、CD3~+淋巴细胞绝对数和百分比,并对两种方法的检测结果进行配对t检验和线性回归分析。选取5例CD3~+低值样本,考察体积法检测CD4~+、CD8~+、CD3~+淋巴细胞绝对数的精密度。结果 Trucount法和体积法对移植术后患者外周血CD4~+、CD4~+/CD3~+、CD8~+、CD8~+/CD3~+、CD4~+/CD8~+检测结果之间差异无统计学意义(P均0.05);线性回归系数均在0.9~1.1之间。CD3~+T细胞≥40个/μL时,体积法检测结果的变异系数均5.5%;当CD3~+T细胞为20个/μL时,CD3~+、CD4~+和CD8~+淋巴细胞的变异系数分别是5.19%、10.28%和6.48%。结论基于流量传感器的单平台计数法对外周血T淋巴细胞计数具有较好的准确性和重复性,可用于对肝/肾移植患者移植术后机体免疫状态的监测。     

初诊多发性骨髓瘤患者外周血淋巴细胞亚群的检测及意义

目的:通过检测多发性骨髓瘤(multiple myeloma,MM)患者外周血淋巴细胞亚群评价MM患者机体的免疫功能状态并分析其与患者预后的相关性。方法:采用流式细胞术检测32例初诊MM患者和24例健康献血者的外周血T淋巴细胞、B淋巴细胞、NK细胞及CD4~+CD25~+调节性T细胞(CD4~+CD25~+Treg);分别采用溴甲酚绿法、透射免疫比浊法检测患者血清白蛋白(albumin,Alb)、β2-微球蛋白(β2 microglobulin,β2-MG)。结果:与健康献血者比较,MM患者外周血淋巴细胞中CD3~+、CD4~+T淋巴细胞比例无显著改变(P0.05),CD8~+T淋巴细胞、NK细胞比例升高(P0.05),CD4~+/CD8~+T淋巴细胞比值、CD19~+B淋巴细胞比例降低(P0.05),CD4~+CD25~+Treg细胞占CD4~+T淋巴细胞的比例明显升高(P0.01)。CD4~+CD25~+Treg细胞占CD4~+T淋巴细胞的比例与MM的疾病分期呈正相关,与MM患者血清中的β2-MG浓度亦呈正相关(P0.05)。结论:MM患者体内在淋巴细胞亚群的异常表达可能与其肿瘤负荷、病情进展及预后有关。CD4~+CD25~+Treg细胞的表达异常可能是MM免疫逃逸的一个重要机制。     

慢性乙型肝炎患者细胞免疫功能的变化与病毒复制的关系研究

目的研究慢性乙型肝炎患者细胞免疫功能变化与HBV-DNA载量之间的关系。方法选择2015年1月至2016年6月收治的慢性乙型肝炎患者88例,选择同期健康体检者40例作为健康对照组,流式细胞术检测CD3~+、CD4~+、CD8~+T淋巴细胞比率,实时荧光PCR检测HBV-DNA,进行比较分析。结果慢性乙型肝炎患者高拷贝组(1.0×105copy/mL)与低拷贝组[(1.0×103)~(1.0×105)copy/mL]分别与健康对照组相比较,CD3~+%、CD4~+%、CD8~+%、CD4~+/CD8~+T淋巴细胞差异均具有统计学意义(P0.05);慢性乙型肝炎患者lgHBV-DNA与CD4~+/CD8~+T淋巴细胞比率比值进行Pearson相关性分析结果表明存在负相关性(r=-0.659,P0.05);HBeAg阳性与阴性慢性乙型肝炎患者相比较,lgHBV-DNA、CD3~+%、CD4~+%、CD8~+%、CD4~+/CD8~+T淋巴细胞差异均有统计学意义(P0.05)。结论机体细胞免疫功能状态与HBV-DNA载量交互作用,互为因果加重肝脏炎症损伤,值得进一步研究探讨。     

桂北地区恶性肿瘤患者外周血T淋巴细胞亚群和淋巴细胞亚群的相关性研究

目的探究桂北地区恶性肿瘤患者外周血T淋巴细胞亚群和淋巴细胞亚群的数值及其临床意义。方法选取184例肿瘤患者,分别设有鼻咽癌组94例,乳腺癌组32例,宫颈癌组36例,肺癌组22例,并选取了40例符合性别年龄区间的健康体检人员作为正常对照组,运用流式细胞仪检测肿瘤各组和正常组的T淋巴细胞亚群表面因子CD3~+、CD4~+、CD8~+细胞绝对计数和CD4~+/CD8~+比值,淋巴细胞亚群表面因子相对计数CD3~+%、CD56~+%、CD19~+%,并进行统计学分析,比较肿瘤组和正常组之间的差异。结果肿瘤组患者T淋巴细胞亚群细胞绝对计数CD3~+、CD4~+、CD8~+和正常组对比有显著差异(P=0.000),CD4~+/CD8~+比值,鼻咽癌有显著差异(P=0.000),乳腺癌(P=0.634)、宫颈癌(P=0.409)、肺癌(P=0.629)差异无统计学意义。淋巴细胞亚群相对计数与正常组对比,乳腺癌、肺癌差异无统计学意义,鼻咽癌CD3~+%(P=0.000)、CD56~+%(P=0.001)有显著差异,CD19~+%差异无统计学意义;宫颈癌CD3~+%(P=0.000)有显著差异,CD56~+%(P=0.094)差异无统计学意义,CD19~+%(P=0.235)差异无统计学意义。结论恶性肿瘤患者免疫功能异常,通过流式细胞仪检测T淋巴细胞亚群、淋巴细胞亚群项目,对患者病情评估、身体免疫情况实时监测、后期治疗指导用药具有一定的临床应用价值。     

喘息性肺炎患儿全血中T淋巴细胞亚群和自然杀伤细胞的表达研究

目的探讨喘息性肺炎患儿全血中T淋巴细胞亚群和自然杀伤细胞的表达及意义。方法选取2013-2016年在中山市人民医院确诊为喘息性肺炎患儿36例作为喘息性肺炎组,另选取同期来该院体检健康儿童36例作为对照组,采用流式细胞仪检测分析两组全血中T淋巴细胞亚群和自然杀伤细胞的表达,并做ROC曲线进行结果分析。结果喘息性肺炎组CD3~+T淋巴细胞、CD3~+CD8~+T淋巴细胞、CD28~+T淋巴细胞、CD8~+CD28~+T淋巴细胞、CD8~+CD28-T淋巴细胞、CD3-CD16~+CD56~+自然杀伤细胞、CD3~+CD16~+CD56~+自然杀伤细胞、CD3~+CD4~+/CD3~+CD8~+与对照组比较,差异有统计学意义(P0.05)。ROC曲线统计结果显示,流式细胞仪检测T淋巴细胞亚群和自然杀伤细胞各参数均有较高的灵敏度。结论喘息性肺炎患儿存在免疫功能障碍,免疫功能的改变与喘息性肺炎发生相关,对喘息性肺炎患儿进行免疫功能检测具有重要的临床意义。     

急性喉炎患儿T淋巴细胞亚群与正常体检儿童之间差异性分析

目的分析急性喉炎患儿T淋巴细胞亚群与正常体检儿童之间差异性,探讨小儿急性喉炎发病初期T淋巴细胞亚群的变化规律。方法选取无其他免疫系统疾病且符合诊断标准的急性喉炎患儿87例,年龄0.3~14岁;另选取同期体检结果正常的健康儿童66例,年龄0.5~13岁。采集患儿血液样本,检测淋巴细胞亚群(CD4~+、CD8~+、CD4~+/CD8~+)、C-反应蛋白(CRP)和白细胞计数(WBC),比较其检测值与健康儿童的体检结果。结果急性喉炎患儿CD8~+T淋巴细胞、CRP及WBC含量分别为52.66%、84.79 mg/L及80.44×109/L,健康儿童分别为73.48%、52.47 mg/L及58.23×109/L,经比较差异有统计学意义(P0.05);急性喉炎患儿CD4~+T淋巴细胞及CD4~+/CD8~+比值分别为54.36%、66.18,健康儿童分别为69.23%、58.13,经比较差异未见统计学意义(P0.05)。结论小儿急性喉炎可引起T淋巴细胞亚群CD8~+升高,CRP、WBC检测值降低,其检测结果可指导临床正确应用免疫调节剂以及抗生素,改善患儿预后。     

葛根素在卵巢早衰患者中的免疫调节作用

目的:探讨葛根素(Puerarin)对卵巢早衰(premature ovarian failure,POF)患者的免疫调节机制。方法:随机选取抗卵巢抗体(AoAb)阳性的POF患者40例,采用葛根素-安宫黄体酮序贯治疗。在治疗前和治疗第12个周期采用Kupperman评分法评价该方法的临床疗效;并检测患者AoAb,CD3~+、CD4~+、CD8~+T淋巴细胞,CD4~+/CD8~+值,CD19~+B淋巴细胞,抗核抗体(ANA)等指标。统计治疗过程中不良反应的发生情况。结果:治疗后患者的Kupperman评分、ANA转阴率、AoAb转阴率和AoAb浓度降低(P0.05)。治疗后CD8~+T细胞数明显增加;而CD3~+、CD4~+T淋巴细胞,CD4~+/CD8~+比值,CD19~+B淋巴细胞数明显减少或降低,差异均有统计学意义(P0.05)。Kupperman评分改变与AoAb,CD3~+、CD4~+T淋巴细胞数,CD19~+B淋巴细胞数,ANA变化正相关(P0.05);Kupperman评分与CD8~+T细胞计数负相关(P0.05)。结论:葛根素-安宫黄体酮序贯疗法可能通过调节CD3~+、CD4~+、CD8~+T淋巴细胞功能来调节机体免疫功能,通过抑制CD19~+B淋巴细胞的增殖及ANA、AoAb等的分泌来消除POF的免疫性损伤,从而实现卵巢的自我修复和功能恢复。     

阴道局部T淋巴细胞的免疫指标变化与宫颈病变关系研究

目的分析阴道局部T淋巴细胞的免疫指标变化与宫颈病变关系。方法选取2015年9月至2016年6月该院门诊、住院部收治的宫颈病变患者60例作为研究对象。人乳头瘤病毒(HPV)感染阳性患者为观察组,HPV感染阴性患者为对照组,分别检测两组患者阴道局部CD4~+T淋巴细胞、CD8~+T淋巴细胞的值及CD4~+/CD8~+比值。结果观察组阴道局部CD4~+T淋巴细胞的值、CD4~+/CD8~+比值低于对照组,差异有统计学意义(P0.05);慢性宫颈炎患者阴道局部CD4~+T淋巴细胞、CD8~+T淋巴细胞的值低于宫颈病变患者,差异有统计学意义(P0.05);慢性宫颈炎患者随访3~6个月,非转阴患者阴道局部CD4~+T淋巴细胞、CD8~+T淋巴细胞的值、CD4~+/CD8~+比值低于转阴患者,差异有统计学意义(P0.05)。结论阴道局部CD4~+T淋巴细胞和CD4~+/CD8~+比值可成为防预HPV感染引起宫颈病变的潜在指标,有助于诊疗和判断患者疾病愈后的情况,值得推广应用。     

巨细胞病毒性肺炎儿童体内淋巴细胞亚群的变化特点

目的研究巨细胞病毒性肺炎儿童体内淋巴细胞亚群的变化。方法收集儿童巨细胞病毒(CMV)肺炎感染者共102例,同时选取15例同龄的健康儿童作为对照组。根据CMV-pp65抗原检测结果将CMV肺炎患儿分为CMV肺炎高活动性感染组和CMV肺炎活动性感染组,使用流式细胞仪检测各组儿童外周血液中CD3~+T淋巴细胞、CD3~+CD4~+T淋巴细胞和CD3~+CD8~+T淋巴细胞的比例。结果在CMV肺炎高活动性感染组患儿体内,CD3~+T淋巴细胞、CD3~+CD4~+T淋巴细胞和CD3~+CD8~+T淋巴细胞的比例分别为(71.35±2.58)%、(30.31±2.47)%和(41.36±1.24)%,与健康对照组相比差异有统计学意义(P0.05);在CMV肺炎活动性感染组患儿体内,CD3~+T淋巴细胞、CD3~+CD4~+T淋巴细胞和CD3~+CD8~+T淋巴细胞的比例分别为(74.89±1.78)%、(45.69±3.11)%和(33.39±2.01)%,与健康对照组相比差异具有统计学意义(P0.05)。结论 CMV肺炎感染患儿体内CD3~+T淋巴细胞和CD3~+CD4~+T淋巴细胞比例下降,CD3~+CD8~+T淋巴细胞比例升高。     

原发性肝癌患者免疫细胞变化及其与HBV-DNA、HBeAg和肿瘤大小的关系

目的探讨原发性肝癌患者机体免疫细胞变化及其与乙型肝炎病毒核酸(HBV-DNA)、乙型肝炎e抗原(HBeAg)和肿瘤大小的关系,为原发性肝癌发病免疫机制的研究提供依据。方法选择2017年7月至2018年12月该院消化科住院的原发性肝癌患者120例为HCC组,选择同期82例健康体检者为对照组。比较两组研究对象外周血T淋巴细胞亚群水平,并比较不同HBV-DNA载量、HBeAg表达及不同肿瘤大小的原发性肝癌患者T淋巴细胞亚群情况。结果 HCC组患者CD3~+T淋巴细胞、CD4~+T淋巴细胞以及CD4~+/CD8~+低于对照组,CD8~+T淋巴细胞高于对照组,差异有统计学意义(P0.05);不同HBV-DNA表达水平者、不同HBeAg表达患者CD3~+T淋巴细胞、CD4~+T淋巴细胞、CD8~+T淋巴细胞以及CD4~+/CD8~+比较,差异均无统计学意义(P0.05);大肝癌组CD4~+/CD8~+低于小肝癌组,差异有统计学意义(P0.05)。结论原发性肝癌患者T淋巴细胞亚群的检测能较好地反映患者的细胞免疫水平。     

Electrochemical detection of hepatitis B surface antigen using colloidal gold nanoparticles modified by a sol-gel network interface

BACKGROUND: A novel potentiometric immunosensor for the detection of hepatitis B surface antigen has been developed by self-assembling gold nanoparticles to a thiol-containing sol-gel network. METHODS: A cleaned gold electrode was first immersed in a hydrolyzed (3-mercaptopropyl) trimethoxysilane sol-gel solution to assemble a three-dimensional silica gel, and then gold nanoparticles were absorbed onto the thiol groups of the sol-gel network. Finally, hepatitis B surface antibody was assembled onto the surface of the gold nanoparticles. The self-assembling procedure was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. Detection is based on the change in potentiometric response before and after the antigen-antibody reaction. RESULTS: Tests relating to the detection of hepatitis B surface antigen demonstrate that the potentiometric immunosensor exhibited a rapid potentiometric response (<4 min), with high sensitivity, good reproducibility, and long-term stability. The linear range was from 4 to 960 ng.mL(-1) with a detection limit of 1.9 ng.mL(-1) (S/N = 3) and the lifetime was 1 month. CONCLUSION: Analytical results of several specimens using the developed technique showed satisfactory agreement with those from an ELISA method. This method shows promise for detecting HBsAg in clinical specimens.     

Obtaining of CD34+ cells from healthy blood donors: development of a rapid and efficient procedure using leukoreduction filters

BACKGROUND: Human CD34+ cells are mandatory to study many aspects of human hematopoiesis. Their low frequency in blood or marrow and ethical reasons limit their obtainment in large quantities. Leukoreduction filters (LRFs) are discarded after preparation of red blood cells. The CD34+ cell concentration in healthy donor blood is low (1 × 10³‐4 × 10³/mL), but their number trapped in one LRF after filtration of 400 to 450 mL of blood is high (0.4 × 10⁶‐1.6 × 10⁶). STUDY DESIGN AND METHODS: To develop a procedure allowing obtainment of purified CD34+ cells from LRFs with a good yield, white blood cell (WBC) recoveries after a 500‐mL continuous or after sequential elution (50‐ or 20‐mL fractions) were compared. Different WBC and mononuclear cell (MNC) centrifugation methods were tested to minimize their PLT contamination before the CD34+ cell immunomagnetic selection. Cell functionality was finally analyzed under various culture conditions. RESULTS: The 20‐mL back‐flushing of LRFs allowed the most efficient WBC recovery. The next steps (110 × g centrifugation, MNC separation on Ficoll, and washes) resulted in a cell suspension in which the lymphocyte recovery was approximately 76 ± 10% and the PLT contamination below 1.6%. After immunomagnetic selection, 4 × 10⁵ to 6 × 10⁵ cells containing approximately 85% of functional CD34+ cells were obtained. CONCLUSION: This procedure allows the easy, rapid (<5 hr), and efficient preparation of large quantities of CD34+ cells having functional activities similar to those of CD34+ cells from other sources. Therefore, easily available and virally safe, LRFs represent an important and regular WBC source to work with human CD34+ cells, but also with other WBC types.     

应用TGF-β_1从rhG-CSF动员的外周血采集物中诱导产生CD4~+ CD25~+调节性T细胞

目的探讨在人重组的粒细胞集落刺激因子(rhG-CSF)动员的外周血单个核细胞中诱导产生CD4+CD25+调节性T细胞可行性及其表型和功能。方法收集本院外周血干细胞移植术供者rhG-CSF动员前外周血(PB组)和动员后的外周血采集物(G-PB组)各10例,用免疫磁珠法分选出CD4+CD25?T细胞,并用转化生长因子β1(TGF-β1)进行诱导,分别应用流式细胞术、RT-PCR和细胞增殖、抑制试验测定诱导后细胞的CD25、Foxp3表达和免疫抑制功能,比较2组之间CD4+CD25+T细胞转化率、抑制功能的差异。结果1)G-PB和PB来源的CD4+CD25?T细胞在抗-CD3 M cAb和TGF-β1作用下CD25+分子表达不同,分别为:(77.9±2.3)%和(65.7±4.2)%,差异有统计学意义(P<0.05);2)TGF-β1诱导产生的CD4+CD25+T细胞高表达Foxp3;3)PB、G-PB来源的TGF-β1诱导产生的CD4+CD25+T细胞具有免疫抑制功能,cpm值分别为:11 739±352和18 732±437(P<0.05)。结论G-CSF动员的外周血可作为CD4+CD25+调节性T细胞的重要来源。     

4种固定破膜试剂盒检测人调节性T淋巴细胞的结果比较

目的比较4种固定破膜试剂盒检测人调节性T淋巴细胞的效果。方法选择CD4、CD25和叉状头转录因子3(Foxp3)3种抗体建立流式调节性T淋巴细胞检测方案。采集10例健康人的新鲜外周血分离单个核细胞,先标记膜表面抗体CD4与CD25,再分别用BD Pharmingen、eBioscience、Invitrogen及Beckman Coulter 4种的固定破膜试剂盒对细胞固定破膜,最后标记Foxp3抗体上机检测。结果与未固定破膜的相应样品比较,4种试剂盒均对细胞的形态有较明显的影响,但对膜表面抗体CD4和CD25的表达没有明显影响。采用eBioscience固定破膜试剂检测调节性T淋巴细胞(CD4~+CD25~+Foxp3~+)的比例为(8.22±2.75)%;Beckman Coulter为(0.54±0.48)%;BD Pharmingen为(0.69±0.33)%;Invitrogen为(0.41±0.24)%。结论 4种试剂盒的固定效果相近,eBioscience固定破膜试剂盒对胞内Foxp3具有较好的检测效果,更适合用于检测调节性T淋巴细胞。     

Does cyclosporin A affect CCR5 and CXCR4 expression in primary HIV-1-infected patients?

BACKGROUND: CCR5 and CXCR4 are the major coreceptors of HIV required for successful viral entry. No information exists on the effect of cyclosporin A (CsA) on expression of CCR5 and CXCR4. A longitudinal study of the coreceptors' expression in freshly isolated peripheral blood mononuclear cells (PBMC) of patients with primary HIV infection (PHI) was performed. METHODS: Patients received highly active antiretroviral therapy (HAART) alone (n = 7) or with CsA (HAART + CsA) (n = 8). Flow cytometric data were analyzed at T0 (baseline), two (T2), six (T6), and twelve (T12) months after therapy initiation. RESULTS: At T0 PHI subjects presented a statistically significant higher count and percentage of CD8+CCR5+ lymphocytes compared to healthy donors (HD) (mean +/- SD, 2,240 +/- 1,998 vs 181 +/- 89 cells/microl). Conversely, CD4+CXCR4+ lymphocytes were less abundant in PHI than in HD (443 +/- 337 vs 673 +/- 339 cells/microl), whereas CD4+CCR5+ lymphocytes were substantially comparable (169 +/- 167 vs 126 +/- 60 cells/microl). In the follow up no differences between HAART and HAART + CsA groups reached statistical significance in CD4 lymphocytes. CD4+CCR5- lymphocytes displayed a rapid recovery after therapy initiation, similarly to the CD4+CXCR4+ subset. In CD8 lymphocytes a statistically significant difference between HAART and HAART + CsA patients occurred at T2 when HAART + CsA patients presented a lower absolute count of the CD8+CXCR4+ subset compared to the HAART group. The major change after therapy initiation in all PHI patients was a striking drop of CD8+CCR5+ lymphocytes; moreover, the CD8+CXCR4- subset behaved similarly. The decrement of CD8+CCR5+ lymphocytes paralleled the decline of viremia and CD8+CD38+ lymphocytes, with the sharpest slope at T2. Conversely, RANTES levels increased at T2 and remained elevated during the follow up. CONCLUSIONS: CsA cotreatment in PHI patients appears not to substantially modify HIV coreceptors' expression in PBMC. However, this novel piece of information should be used with caution, since this was not a randomized study between the HAART and the HAART + CsA groups.     

乙肝病毒血清标志物、外周血T淋巴细胞与HBV-DNA的相关性分析

目的 探讨乙肝病毒血清标志物、外周血T淋巴细胞与HBV-DNA的相关性。方法 273例乙肝患者采用ELISA法测定乙肝血清标志物HBsAg,HBsAb,HBeAg,HBeAb和HBcAb,采用流式细胞术测定T淋巴细胞亚群CD3⁺,CD4⁺和CD8⁺,并采用荧光定量PCR法测定HBV-DNA,比较不同血清标志物模式间HBV-DNA阳性表达情况,并分析其相关性。结果 HBsAg(+)HBeAg(+)HBcAb(+)组的HBV-DNA阳性率最高,为88.9%,HBV-DNA高浓度组CD3⁺ CD4⁺细胞百分比及CD3⁺CD4⁺/CD3⁺ CD8⁺比值低于HBV-DNA阴性组(P<0.05),CD3⁺ CD8⁺细胞百分比高于HBV-DNA阴性组(P<0.05); HBV-DNA拷贝数与CD3⁺ CD8⁺细胞百分比及HBeAg(r²=0.550,0.657,P<0.01)呈正相关,与CD3⁺ CD4⁺细胞百分比(r²=-0.602,P<0.05),HBeAb(r²=-0.473,P<0.01),HBcAb(r²=-0.151,P<0.05)呈负相关。结论 HBV-DNA与T淋巴细胞及HBeAg检测相关性较好,联合检测乙肝血清标志物、外周血T淋巴细胞及HBV-DNA能提高乙肝的检出率,有助于全面监测乙肝病情及评价药物疗效。     

Comparison of hematopoietic progenitor cell collections using the COBE Spectra version 7 and Amicus version 3.1 for patients with AL amyloidosis

To address concerns about infused fluid volume during HPC collections in patients with AL amyloidosis, our institution has used a 26:1 anticoagulant (AC) ratio on the COBE Spectra and on the Fenwal Amicus. In this study, in a cohort of AL amyloid patients, we compared the Amicus version 3.1 to the Spectra version 7 MNC collections with regard to infused fluid volume, CD34+ cell yield, lymphocyte yield, cross‐cellular content, and adverse reactions. Both instruments used a 26:1 AC ratio but the Amicus delivered significantly less AC per procedure (Amicus 678 mL vs. Spectra 753 mL). With comparable baseline CD34+ cell counts (Amicus 33 cells/μL vs. Spectra 27 cells/μL); Amicus collected significantly more CD34+ cells (3.1 vs. 1.5 × 10⁶/kg) and equivalent lymphocytes (18.7 vs. 14.5 × 10⁹). Amicus collected significantly fewer WBC (51.8 vs. 72.7 × 10⁹), granulocytes (15.1 vs. 27.5 × 10⁹), and PLT (2.3 vs. 3.9 × 10¹¹) per procedure with equivalent RBC content (26 vs. 30 mL). CD34+ cell (5.0 vs. 4.4 × 10⁶/kg) and lymphocyte doses (32.7 vs. 33.9 × 10⁹) were equivalent in infused products collected on the Amicus and Spectra but the frequency of high volume products was lower for Amicus. Frequency and severity of adverse reactions during collection and infusion were similar for both. In this group of AL amyloid patients, Amicus was superior to Spectra with regard to fluid infused, CD34+ cell yield, and cross‐cellular contamination with equivalent lymphocyte yield and reaction incidence. © 2011 Wiley‐Liss, Inc.     

初治弥漫大B细胞淋巴瘤患者化疗后T淋巴细胞亚群变化

目的 探讨弥漫大B细胞淋巴瘤患者的细胞免疫状态改变及其预测预后的意义。方法 回顾性分析复旦大学附属中山医院厦门医院血液科2018年2月至2020年12月收治的28例初治弥漫大B细胞淋巴瘤患者。用流式细胞仪检测患者化疗前后不同时期外周血T淋巴细胞亚群变化。结果 与化疗前相比，患者化疗后外周血CD4⁺T淋巴细胞计数及CD4⁺/CD8⁺比值均降低。CD4⁺T淋巴细胞计数在化疗4个周期及6个周期后显著低于化疗前(P<0.05)，在化疗4个周期后达最低值[(0.245±0.086)×10⁹/L]。CD4⁺/CD8⁺比值在化疗4个周期后、化疗6个周期后、化疗结束后3个月及化疗结束后6个月显著低于化疗前(P<0.05)。终末疗效为完全缓解的患者化疗6个周期后CD4⁺T淋巴细胞计数及CD4⁺/CD8⁺比值高于非完全缓解患者(P<0.05)。复发患者化疗6个周期后CD4...     

流式细胞术分析神经系统疾病患者脑脊液T淋巴细胞亚群

目的用流式细胞术分析脑脊液中T淋巴细胞及其亚群,探讨不同类型神经系统疾病患者脑脊液中T淋巴细胞亚群百分率差异,寻找在疾病诊断和治疗监测中有意义的实验室检测指标。方法采用流式细胞术测定神经系统疾病患者脑脊液和外周血T淋巴细胞亚群。结果采用流式细胞术分析脑脊液中T淋巴细胞亚群时,运用侧向散射光(SS)vs.CD45能更准确的确定淋巴细胞群;但流式细胞术只适用于检测神经系统免疫相关疾病患者脑脊液中T淋巴细胞亚群,不适用于分析神经系统变性疾病患者。中枢神经系统炎性疾病和脊髓炎患者脑脊液中CD3+T淋巴细胞、CD3+CD4+T淋巴细胞和CD3+CD8+T淋巴细胞百分率最高,脱髓鞘疾病患者脑脊液T淋巴细胞亚群百分率最低,但各类神经系统疾病患者脑脊液中T淋巴细胞百分率无明显差异(P>0.05)。细菌性脑膜炎患者脑脊液中CD3+T淋巴细胞和CD3+CD4+T淋巴细胞百分率最高,明显高于脱髓鞘疾病患者(P=0.032),但与病毒性脑膜炎患者比较差异无统计学意义(P>0.05)。各类神经系统疾病患者脑脊液中CD4/CD8比值明显高于外周血,其中细菌性脑膜炎和脊髓炎患者脑脊液中CD4/CD8比值最高,脱髓鞘疾病脑脊液中CD4/CD8比值最低,但差异均无统计学意义(P>0.05)。脑脊液IgG生成指数与脑脊液中CD3+CD4+T淋巴细胞百分率有一定相关性(r=0.324,P=0.032)。结论流式细胞术分析神经系统炎性疾病较脑脊液常规检测具有优势,敏感性更高,可同时分析脑脊液免疫状况。流式细胞术分析在神经系统相关炎性疾病具有重要意义,CD3+CD4+T淋巴细胞高百分率与神经系统炎性疾病密切相关。但流式细胞术对于神经系统变性疾病的临床应用有限。     

CD81和趋化因子CC受体5与丙型肝炎合并人类免疫缺陷病毒感染的相关性研究

目的 检测外周血CD4＋及CD8＋T淋巴细胞表面CD81、CCR5的表达,探讨与丙型肝炎病毒（HCV）单纯感染、人类免疫缺陷病毒（HIV）单纯感染和HCV/HIV合并感染的关系.方法 采用流式细胞术,检测HCV感染组（n=21）、HIV感染组（n=14）、HCV/HIV感染组（n=28）及正常对照组（n=30）人外周血CD4＋及CD8＋T淋巴细胞表面CD81和CCR5的表达.结果 与正常对照组相比,外周血CD4＋T淋巴细胞表面CD81,在HCV感染组表达变化不明显,而在HIV感染组和HCV/HIV合并感染组中低表达;CD8＋T淋巴细胞表面CD81,在HCV感染组、HIV感染组和HCV/HIV合并感染组中都呈高表达.外周血CD4＋T和CD8＋T淋巴细胞表面CCR5,在HIV感染组高表达,而在HCV感染组和HCV/HIV合并感染组中低表达.结论 HCV/HIV合并感染中,影响外周血CD4＋及CD8＋T淋巴细胞表面CCR5表达的主要因素是HCV感染;而合并感染对CD4＋及CD8＋T淋巴细胞表面CD81的表达,其影响作用是不相同的.