Immune response of sows and their offspring to pseudorabies virus: serum neutralization response to vaccination and field virus challenge

One month prior to breeding, sows were vaccinated with an attenuated pseudorabies virus vaccine or challenged with a field strain of pseudorabies virus. A third group of sows were not vaccinated or challenged before breeding. Pigs from these sows were vaccinated at 3, 6, or 12 weeks of age and challenged with virulent virus three weeks later. One pig from each litter served as an unvaccinated, unchallenged control. Serum neutralization titers of these pigs were monitored from birth until 22 weeks of age. Titers of the sows were monitored through breeding, gestation and farrowing. The maximum prefarrowing anti-pseudorabies virus titer in the field virus challenged sows occurred four weeks following challenge. A significant decline in titers occurred at farrowing. Titers rose from one week postfarrowing and then declined. Titers in the field virus infected sows were consistently two to threefold greater than those of the vaccinated sows. The maximum prefarrowing anti-pseudorabies virus titer in the vaccinated sows occurred six weeks following vaccination. The geometric mean titer in these sow's then decreased and increased for two weeks after farrowing. The results in the pigs can be summarized as follows: Pigs from control sows had a greater serological response following field virus challenge than following vaccination with a modified live virus. Pigs from control sows responded serologically to vaccination at 3, 6 and 12 weeks of age. Pigs from control sows which were challenged at 6, 9 and 15 weeks of age had similar antibody responses. Pigs from vaccinated sows had no increase in titer following vaccination at three and six weeks of age. Titers increased when these pigs were vaccinated at 12 weeks of age. There was no significant increase in mean titers of pigs from challenged sows following vaccination at 3, 6 and 12 weeks of age. Vaccinated pigs from control and vaccinated sows had a secondary response following challenge three weeks after vaccination.(ABSTRACT TRUNCATED AT 250 WORDS)     

小鼠体内~(147)Pm的胎盘、乳汁转移及分布

本文研究了~(147)Pm 从妊娠晚期及泌乳BALB/C 小鼠向子代的转移,并比对其在母鼠体内主要沉积器官的分布。于BALB/C 雌性小鼠妊娠17天及分娩后1天,经尾静脉注射~(147)Pm 硝酸溶液。分别于注射后1、4、9、14和21天活杀,分析测量其在母鼠肝脏、右侧股骨、子宫,仔鼠、胎盘及胎膜中的含量。结果显示,妊娠和泌乳动物仔鼠中的~(147)Pm 含量随时间延长而增高,且泌乳动物仔鼠体内~(147)Pm 含量在注射后1天和4天分别比妊娠动物仔鼠高20多倍。妊娠晚期注射动物于1和4天,胎盘及胎膜~(147)Pm 含量均明显高于仔鼠。妊娠和泌乳组肝脏中~(147)Pm 生物半排期比对照组动物为高,骨骼中~(147)Pm 也明显高于对照动物。     

Maternal fatness and viability of preterm infants

To investigate the effect of maternal fatness on the mortality of infants born preterm up to the corrected age of 18 months 795 mother-infant pairs were studied. Maternal fatness was defined by Quetelet's index (weight/(height] and all infants weighed less than 1850 g at birth. In 771 mother-infant pairs maternal age, complications of pregnancy, mode of delivery, parity, social class, and the baby's sex and gestation were analysed by a logistic regression model for associations with infant mortality (but deaths from severe congenital abnormalities and those occurring during the first 48 hours after birth were excluded). In a subgroup of 284 mother-infant pairs all infant deaths except those from severe congenital abnormalities were analysed in association with the infant's birth weight and gestation and the mother's height and weight; this second analysis included another 24 infants who had died within 48 hours after birth. In the first analysis mortality overall was 7% (55/771), rising from 4% (71/173) in thin mothers (Quetelet's index less than 20) to 15% (6/40) in mothers with grades II and III obesity (Quetelet's index greater than 30). After adjusting for major demographic and antenatal factors, including serious complications of pregnancy, maternal fatness was second in importance only to length of gestation in predicting death of infants born preterm. In the second analysis mortality overall was 15% (44/284), rising from 9% (5/53) in thin mothers to 47% (8/17) in mothers with grades II and III obesity. In both analyses the relative risk of death by 18 months post-term was nearly four times greater in infants born to obese mothers than in those born to thin mothers. In addition, maternal fatness was associated with reduced birth weight, whereas it is associated with macrosomia in term infants. These data differ fundamentally from those reported in full term babies of obese mothers. It is speculated that the altered metabolic milieu in obesity may reduce the ability of the fetus to adapt to extrauterine life if it is born preterm.     

A case control study of the prevalence of perinatal complications associated with fetal macrosomia in Antigua and Barbuda

The prevalence of obstetric, perinatal and neonatal complications associated with fetal macrosomia at Holberton Hospital in Antigua and Barbuda was assessed by a retrospective, case-control study. All babies of birthweight (BW) greater than 4.5 kg born between July 1991 and January 1997 and all babies with BW greater than 4.0 kg born between July 1991 and January 1995, were included. Control babies, were selected from those born on the same day as the index case. Babies of BW > 4.0 kg and babies of BW > 4.5 kg were 5.7% and 1% of births respectively Records were complete for 157 large babies (40 with BW > 4.5 kg) and 157 control babies < 4.0 kg. Mothers of large babies were significantly older, more parous, more likely to have diabetes mellitus, hypertension, and deliver after 40 weeks gestation. At delivery, mothers of large babies were more likely to bleed. Large babies had lower one minute and five minute Apgar score, were more likely to be meconium stained, have respiratory distress, have birth trauma or dystocia and to be admitted to Special Care Nursery. There was no difference in Caesarean section rate, hospital days, neonatal jaundice or mortality. Babies with BW > 4.5 kg had mortality of 7.5% versus 1.8% for those < 4.5 kg. Fetal macrosomia remains a difficult obstetrical problem associated with significant maternal, perinatal and neonatal consequences. Morbidity and mortality are still significant in developed and developing countries alike.     

子午沙鼠的室内繁殖特征

目的了解和掌握子午沙鼠在实验室内繁殖特征。方法根据1996~2002年实验室饲养的子午沙鼠记录数据,推导雌雄体的性成熟期和雌体妊娠期,统计分析不同胎次和月份每窝产仔数、离乳数、成活率和性比结果。结果雌雄体的性成熟期分别为(109.3±21.0)d和(106.3±21.7)d,妊娠期为(21.3±1.4)d;1~7胎各胎次之间出生数和离乳数差异不显著(P0.05),而第4胎和第6胎的成活率低于平均胎仔数,但差异无显著性(P0.05);1~7胎之间雌雄百分比差异没有显著性(P0.05),各胎次平均雌雄比例为1.4∶1.0。在12个月份中,9月-11月停止繁殖,其它各月份之间每窝出生数之间差异不显著(P0.05),每窝离乳数和成活率在部分月份之间差异有显著性(P0.05)。结论与野生子午沙鼠繁殖数据比较,实验室驯化对子午沙鼠繁殖有影响,主要为季节繁殖提前到12月,繁殖窝数增加1~2窝。本结果为该鼠种实验动物化研究提供参考。     

Piglet blood glutathione peroxidase levels and preweaning mortality

The blood glutathione peroxidase levels of one day old piglets from 22 litters were examined. Body weight and piglet survival were monitored in order to assess the relationship between these two factors and blood glutathione peroxidase activity. The mean blood glutathione peroxidase level of one day old piglets (65 mu/gHb) was significantly lower (p0.001) than the mean level (85 mu/gHb) at weaning. The mean blood glutathione peroxidase activity of one day old piglets was not related to the size of the litter, but was related (p less than 0.1) to the mean litter blood glutathione peroxidase level at weaning time. Piglet blood glutathione peroxidase was not related to piglet body weight. The blood glutathione peroxidase level of the sows at one-day post-farrowing was not related to the mean blood glutathione peroxidase activity of their litters at one day of age but was correlated (p less than 0.1) with the mean blood glutathione peroxidase levels of their litters at weaning. Piglet viability was shown to be strongly correlated (p less than 0.001) with body weight at one day of age. The blood glutathione peroxidase level of one day old piglets was weakly associated (p less than 0.1) with piglet survival. Further work is required to clarify this latter observation, which suggests that selenium supplementation to newborn piglets may be beneficial regardless of the dams nutritional status.     

Is grand multiparity an independent predictor of pregnancy risk? A retrospective observational study

OBJECTIVE: To determine whether high maternal parity has any effect on pregnancy outcome independent of other maternal characteristics. DESIGN AND SETTING: Retrospective observational study using the database of a referral obstetric unit in a 280-bed regional hospital in far north Queensland. PARTICIPANTS: All 15 908 women who had singleton births between 1992 and 2001, comprising 653 women with grand multiparity (>or= 5 previous births at gestation >or= 20 weeks) and 15 255 women with lower parity. MAIN OUTCOME MEASURES: Spontaneous vaginal birth, postpartum haemorrhage (estimated blood loss > 500 mL), placental retention requiring manual removal, blood transfusion associated with the birth, and perinatal death. RESULTS: Women with grand multiparity were significantly older than those with lower parity, more likely to be Indigenous, not to have had antenatal care, to have smoked during pregnancy and to have had one or more previous caesarean sections. On univariate analysis, women with grand multiparity were more likely to have a postpartum haemorrhage (9.2% v 5.3%) and blood transfusion (2.8% v 1.5%). However, multivariate logistic regression analysis of women who began labour (ie, did not have an elective caesarean section) showed that grand multiparity was not significantly associated with postpartum haemorrhage or blood transfusion when other maternal characteristics were included in the model (regression coefficients [95% CI], 1.36 [0.99-1.87] and 1.09 [0.59-2.02], respectively). However, they remained more likely to have a spontaneous vaginal birth (regression coefficient [95% CI], 2.10 [1.56-2.74]). CONCLUSIONS: Women with grand multiparity do not have an increased likelihood of poor pregnancy outcomes. Birth-suite protocols which dictate extra interventions as routine during labour in these women should be revised.     

Australian national birthweight percentiles by gestational age

OBJECTIVE: To develop national birthweight percentiles by gestational age for male and female singleton infants born in Australia, and to compare the birthweight percentiles of Indigenous and non-Indigenous infants. DESIGN AND SETTING: Cross-sectional study of singleton live births to Australian-born mothers from 1991 to 1994. MAIN OUTCOME MEASURES: Birthweight percentiles by gestational age. RESULTS: During 1991-1994 Australian-born women gave birth to 769,077 live singleton infants. Of these, 28,230 (3.7%) were reported as births to Aboriginal or Torres Strait Islander women. Birthweight was missing for 581 (0.1%) births and gestational age was missing for 3014 (0.4%). An additional 3283 (0.4%) births were excluded because the recorded birthweights were extreme outliers for their recorded gestational ages. Indigenous women were more likely to be recorded as giving birth preterm (< 37 weeks' gestation) than non-Indigenous women (11.6% v. 5.4%) and were more likely to give birth to small-for-gestational-age infants at term. After 34 weeks' gestation, the median birthweights of Indigenous infants were consistently lower than those of non-Indigenous infants. At 40 weeks' gestation the difference in the median birthweights between these two groups was 160 g for males and 130 g for females. CONCLUSIONS: We present recent birthweight percentiles by gestational age based on national data in Australia. These percentiles provide current Australian norms for clinicians and researchers, and can provide a baseline for monitoring Indigenous perinatal outcomes.     

Teenage smoking in pregnancy and birthweight: a population study, 2001-2004

OBJECTIVE: To determine the association between smoking in pregnant teenagers and baby birthweight. DESIGN, SETTING AND PARTICIPANTS: A retrospective population-based study of women aged < 20 years who gave birth to liveborn singletons in Australia between January 2001 and December 2004. Data were drawn from the National Perinatal Data Collection. MAIN OUTCOME MEASURES: Maternal smoking, birthweight, low birthweight (LBW). RESULTS: The prevalence of LBW in babies born to teenage smokers was 9.9%, compared with 6.0% in babies born to teenage non-smokers (odds ratio [OR], 1.72 [95% CI, 1.57-1.90]). On average, babies born to teenage smokers were 179.8 g lower in birthweight than babies born to teenage non-smokers (95% CI, 165.5 -194.1 g; t = 24.6, P < 0.001). Smoking, Indigenous status, Socio-Economic Indexes for Areas category and parity were independently associated with LBW (all ORs > 1.3; P < 0.001) after adjusting for maternal age group. Teenagers smoking > 10 cigarettes a day had babies with lower birthweight that those who smoked < or = 10 cigarettes a day, demonstrating a dose-response relationship. The babies of teenage smokers who stopped smoking before 20 weeks' gestation had birthweights similar to those of babies born to teenage non-smokers. One in 15 teenage smokers stopped smoking during pregnancy. CONCLUSION: Babies whose mothers smoked during pregnancy were more likely to have LBW than babies whose mothers did not smoke. Mothers who continue to smoke in the second half of pregnancy increase their baby's risk of LBW. There is significant scope to improve the quitting rate, and health professionals need to target smoking cessation at all contacts with pregnant women who continue to smoke.     

The clinical utility of a published nomogram to predict aminoglycoside nephrotoxicity

Moore and colleagues recently published a nomogram for predicting the risk of aminoglycoside nephrotoxicity. This study was undertaken to evaluate the clinical usefulness of this nomogram. The nephrotoxicity risk scores of 61 patients were determined using parameters suggested by Moore et al. Thirteen patients (21%) could not be scored because their initial creatinine clearances exceeded the upper limit of the nomogram. Six patients (9.8%) developed nephrotoxicity (defined as a greater than or equal to 50% decrease in creatinine clearance). In five of these patients in whom risk scores could be determined, the scores were estimated at 14% or less. Twenty patients had risk scores of 10% or more (four patients, greater than or equal to 50%) without developing nephrotoxicity. These results demonstrate that this nomogram may have limited clinical application in predicting patients likely to develop aminoglycoside nephrotoxicity.     

催乳素受体基因多态性对西藏小型猪繁殖性能的影响

目的了解催乳素受体（PRLR）基因AIuI位点在西藏小型猪中的多态性分布情况;研究其在西藏小型猪上的遗传效应,为生长繁殖性状的分子遗传标记辅助选择提供实验依据。方法收集44头西藏小型猪母猪窝产仔数及离乳仔数数据,测定所产仔猪的初生重和离乳重,采用PCR-RFLP方法测定PRLR基因AIuI位点多态性,并与上述生长繁殖性能指标进行相关性分析。结果西藏小型猪PRLR侯选基因AIuI位点表现出多态性,分为AA、AB、BB,频率分别为0.273、0.500、0.227。经产母猪的仔猪初生重在不同基因型间存在显著性差异,而初产和经产母猪的其他生长繁殖指标在不同基因型间均未达到显著性水平。结论本研究中PRLR基因AIuI位点多态性AA、AB、BB不同基因型之间窝产仔数和离乳仔数差异不显著。     

Aboriginal and white births in Western Australia, 1980-1986. Part I: Birthweight and gestational age

This article describes the distributions of birthweight and gestational age of all singleton Aboriginal and white live-born infants in Western Australia for the period 1980-1986. At early gestational ages, the mean birthweight was greater for Aboriginal infants. However, after 34-weeks' gestation for male infants and 32-weeks' gestation for female infants, the pattern was reversed. More Aboriginal infants were of low birthweight--male Aboriginal infants, 9.8%; male white infants, 4.0%; female Aboriginal infants, 12.4%; and female white infants, 4.6%, this excess only occurred in term (37- to 41-weeks' gestation) and post-term (42- to 52-weeks' gestation) infants. The birthweight distributions for Aboriginal and white infants were similar in preterm infants, but at term and beyond Aboriginal infants tended to be lighter. Preterm (fewer than 37-weeks' gestation) births were more common among Aborigines (male Aboriginal infants, 16.0%; white male infants, 6.8%; female Aboriginal infants, 15.9%; and female white infants, 6.0%). Thus, it seems that the distributions of both birthweight and gestational age in Aboriginal infants are shifted downward compared with those for white infants. Aboriginal infants normally may be smaller and more likely to be born earlier than are white infants as well as having a definite shift towards pathological growth retardation at term and beyond.     

Incidence of tuberculosis among reported AIDS cases in Quebec from 1979 to 1996

BACKGROUND: The impact of HIV infection on tuberculosis (TB) rates in Quebec has not been fully established. Because concurrent HIV infection is the single most important factor in TB reactivation, the authors used Quebec AIDS surveillance data to quantify the extent of TB among reported AIDS cases and to identify the characteristics of AIDS patients with TB. METHODS: The study population comprised people aged 15 years and over with AIDS diagnosed between Jan. 1, 1979, and Dec. 31, 1996, and reported by Mar. 13, 1997. Patients with TB (all forms) and those without TB were compared. Multivariate logistic regression analysis was used to examine the independent effect of each variable on the AIDS-TB cases. The authors also compared the number of AIDS-TB cases with the number of TB cases to estimate the effect of HIV infection on TB incidence. RESULTS: Of the 4684 people with AIDS reported in Quebec, 242 (5.2%) had active TB at some point during the course of their illness. During 1992-1995, 9.6% of the people with TB in Montreal, and 5.8% in the province of Quebec, also had HIV infection. Those with AIDS and TB were predominantly male (75.2%), manual workers (40.1%) and residents of Montreal (86.4%) and were born in an HIV-endemic country (63.8%). The multivariate analysis indicated that AIDS patients who were born in HIV-endemic countries in the Caribbean, sub-Saharan Africa or other developing regions were 21.8 times (95% confidence interval [CI] 19.5-28.5), 17.9 times (95% CI 12.7-27.1) and 4.9 times (95% CI 3.5-7.0) more likely to have TB than those born in Canada; manual workers and unemployed people with AIDS were 1.6 times (95% CI 1.3-2.0) and 2.0 times (95% CI 1.5-2.6) more likely to have TB than professional workers; and people who acquired HIV infection through heterosexual contact were 2.1 times (95% CI 1.6-3.1) more likely to have TB than men who acquired it through sexual contact with other men. INTERPRETATION: AIDS seems to contribute significantly to the number of TB cases. The results of this study reinforce the importance of offering HIV testing to people in high-risk groups, such as those born in a country where HIV and TB is endemic.     

The urban-remote divide for Indigenous perinatal outcomes

OBJECTIVE: To determine whether remoteness category of residence of Indigenous women affects the perinatal outcomes of their newborn infants. DESIGN AND PARTICIPANTS: A population-based study of 35 240 mothers identified as Indigenous and their 35 658 babies included in the National Perinatal Data Collection in 2001-2004. MAIN OUTCOME MEASURES: Australian Standard Geographical Classification remoteness category, birthweight, Apgar score at 5 minutes, stillbirth, gestational age and a constructed measure of perinatal outcomes of babies called "healthy baby" (live birth, singleton, 37-41 completed weeks' gestation, 2500-4499 g birthweight, and an Apgar score at 5 minutes >or= 7). RESULTS: The proportion of healthy babies in remote, regional and city areas was 74.9%, 77.7% and 77.6%, respectively. After adjusting for age, parity, smoking and diabetes or hypertension, babies born to mothers in remote areas were less likely to satisfy the study criteria of being a healthy baby (adjusted odds ratio [AOR], 0.87; 95% CI, 0.81-0.93) compared with those born in cities. Babies born to mothers living in remote areas had higher odds of being of low birthweight (AOR, 1.09; 95% CI, 1.01-1.19) and being born with an Apgar score < 7 at 5 minutes (AOR, 1.63; 95% CI, 1.39-1.92). CONCLUSIONS: Only three in four babies born to Indigenous mothers fell into the "healthy baby" category, and those born in more remote areas were particularly disadvantaged. These findings demonstrate the continuing need for urgent and concerted action to address the persistent perinatal inequity in the Indigenous population.     

Bovine viral diarrhea virus in swine: neutralizing antibody in naturally and experimentally infected swine

Neutralizing antibodies against bovine viral diarrhea virus (BVDV) were detected in sera of Iowa farm sows, their piglets derived by hysterectomy and in specific-pathogen-free (SPF) pigs in a closed herd. No antibodies against BVDV were detected in sera of 134 fetal pigs from 67 sows in an Iowa slaughter-house. Neutralizing antibodies against BVDV or hog cholera virus, produced experimentally in SPF pigs, were demonstrated to be due to different antigens rather than to a common antigen, although slight cross-neutralization reactions sometimes occur.     

Efficacy of phototherapy in non-haemolytic hyperbilirubinaemia

Clinical experience of phototherapy for non-haemolytic hyperbilirubinaemia in 3999 infants in Kandang Kerbau Hospital, Singapore, is documented. Phototherapy was most effective in extremely preterm infants with very low birth weight (gestation less than or equal to 32 weeks, birth weight less than or equal to 1500 g) and least effective in full term infants with very low birth weight (gestation greater than or equal to 37 weeks, birth weight less than or equal to 1500 g) and large preterm infants (gestation less than 37 weeks, birth weight greater than 2270 g). Overall, phototherapy was effective in almost all the infants, with a failure rate of only 2.00/1000 infants. No characteristic features common to all the failures could be detected. The bilirubin rebound was usually mild; repeat phototherapy was required in only 30 infants (7.50/1000), with the response to the second exposure comparable to that to the first. No infant required a third exposure. All the infants tolerated phototherapy well, none developing any illness that could be attributed to the treatment. This clinical experience shows that phototherapy for the treatment of nonhaemolytic hyperbilirubinaemia is effective and safe.     

The Relationship of Pregnancy, Vaginal Candidiasis and Glucose Metabolism

In 72 pregnant women with culture-proved vaginal candidiasis, and an equal number of controls matched for parity, size, stage of gestation and age, the intravenous glucose tolerance curves were compared using Silverstone's method. The results in both study and control groups did not differ and resembled closely those in Silverstone's series. Half the study group were treated using nystatin alone, while the other half also received tolbutamide. The cure rate was equal in the two groups. Candidiasis in pregnancy does not appear to be related to altered glucose metabolism nor does the addition of this hypoglycemic drug (tolbutamide) improve results. No ill effects, fetal or maternal, were apparent following the use of tolbutamide.     

The postulated role of feeder swine in the perpetuation of the transmissible gastroenteritis virus.

Clinical, immunofluorescence and histopathological observations were found to be an efficient approach for the confirmation of the diagnosis of transmissible gastroenteritis in feeder swine. Two cases are reported to exemplify how feeder swine exposed to points of concentration such as holding areas, sales barns and auctions can play an important role in the epizootiology of transmissible gastroenteritis. A third field case is reported as an example of an outbreak of transmissible gastroenteritis beginning in feeder swine and then spreading to baby pigs on the farm. All baby pigs died that were born during the acute phase of the outbreak in the feeder swine. Baby pigs born shortly after the clinical signs had abated in the herd, and from sows that had been exposed orally to virulent transmissible gastroenteritis virus and vaccinated with a commercial transmissible gastroenteritis vaccine ten days before farrowing, survived. This was explained by a combination of a decrease in the amount of virus shed in the environment and the immunity induced in the sows. These observations of field outbreaks of transmissible gastroenteritis combined with recently reported experimental studies lend strong support to the hypothesis of a reservoir for transmissible gastroenteritis virus in feeder pigs. This reservoir would be based principally on the transmission of the virus on a continuous basis from the feces of recently infected pigs to susceptible pigs. Clinical signs of transmissible gastroenteritis in such pigs are difficult to recognize or absent and this contributes to the importance of the reservoir in the field.     

新生儿体重与孕妇年龄和体重指数的关系

目的：分析母亲年龄与新生儿出生体重的关系,以及孕期体重指数对新生儿出生体重的影响。方法：回顾性分析2005年1-12月在重庆医科大学附属第一医院产科出生的1301例新生儿体重;以及1999年1月至2003年7月在我院住院并进行阴道试产的初产妇576例,其中早孕期肥胖即体重指数（BMI）≥24有184例,分娩时肥胖（BMI）≥27有152例。观察出生体重与孕妇年龄和体重指数之间的关系。结果：在排出了妊娠合并糖尿病,非足月妊娠以及双胎、三胎妊娠,1301例新生儿平均出生体重为3396g．男婴平均出生体重为3453g,女婴为3397g,其中只有20-24岁组分娩的新生儿出生体重低于总体平均体重;早孕期肥胖孕妇巨大儿的发生率与对照组比较,差异有显著性,分娩时肥胖的产妇巨大儿的发生率与对照组比较,差异有显著性,早孕期体重增加≥15kg者巨大儿的发生率高于体重增加〈15kg者,差异有显著性。结论：母亲年龄过小影响胎儿生长发育,孕期母亲体重增长过多可导致巨大儿发生率增加。     

盆底功能障碍性疾病的多因素分析

目的：探讨盆底功能障碍性疾病的相关危险因素。方法：选取2007年1月至2013年12月于郑州大学第三附属医院盆底重建科行盆底重建术的患者346例为盆底功能障碍性疾病组,体检的健康女性349例为对照组。分别记录其孕产次数、绝经史、子宫切除术手术史、子宫肌瘤病史及白带常规结果。结果：盆底功能障碍性疾病组有子宫切除术史、绝经史、白带常规清洁度≥III度及阴道乳酸杆菌缺乏的患者例数明显高于对照组(P<0.05);盆底功能障碍性疾病组孕次和产次均明显高于对照组(P<0.05)。其中子宫切除手术史、孕次、产次及阴道乳酸杆菌的缺乏是盆底功能障碍性疾病的独立危险因素。结论：有子宫切除术史、孕产次数多、白带常规清洁度≥III度及阴道乳酸杆菌缺乏可能参与了盆底功能障碍性疾病的发生发展,而阴道内乳酸杆菌的存在可能是盆底功能障碍性疾病的保护性因素。