豆腐果苷抗抑郁作用的初步研究

目的初步探讨豆腐果苷的抗抑郁作用。方法采用小鼠悬尾实验及小鼠强迫游泳实验方法观察豆腐果苷的抗抑郁作用。结果在小鼠悬尾实验和小鼠强迫游泳实验中,豆腐果苷在20、30mg/kg剂量下能明显缩短小鼠的悬尾不动时间,在30、40mg/kg剂量下能明显缩短小鼠强迫游泳的不动时间。结论豆腐果苷在“行为绝望”动物模型中呈现抗抑郁作用,且量效曲线呈现行为学特有的U型曲线特征。     

槟榔壳总酚类提取物抗抑郁作用研究

目的:探讨槟榔壳总酚类提取物对抑郁模型小鼠的抗抑郁作用。方法:采用小鼠悬尾、强迫游泳等抑郁模型,以小鼠行为绝望的不动时间作为指标,考察槟榔壳总酚类抗抑郁活性。结果:槟榔壳总酚类320,160 mg.kg-1剂量组均能显著减少小鼠悬尾和强迫游泳的不动时间。结论:槟榔壳总酚类可以改善小鼠的绝望行为,具有明显的抗抑郁作用。     

酸枣仁总皂苷抗抑郁作用的实验研究

目的研究酸枣仁总皂苷对行为绝望小鼠抑郁模型的影响。方法采用小鼠强迫游泳实验和悬尾实验抑郁模型,小鼠行为绝望的不动时间作为指标,考察酸枣仁总皂苷抗抑郁活性。结果酸枣仁总皂苷中、高剂量组均能减少小鼠强迫游泳和悬尾不动时间,与空白对照组比较差异有统计学意义(P<0.05,P<0.01)。结论酸枣仁总皂苷具有一定的抗抑郁作用。     

人参总皂苷对小鼠的抗抑郁作用

目的：初步探讨人参总皂苷对小鼠的抗抑郁作用。方法：选取健康雄性昆明种小鼠,随机分为5组,空白对照组、阳性药组、人参总皂苷125、250、500 mg.kg^-1剂量组。通过小鼠自主活动实验、小鼠强迫游泳实验和小鼠悬尾实验,观察人参总皂苷对小鼠抗抑郁作用的影响。结果：各给药组小鼠自主活动行为与空白对照组比较均无明显差异;人参总皂苷125、250、500 mg.kg-1均可以显著缩短小鼠强迫游泳及小鼠悬尾不动时间。结论：实验结果表明人参总皂苷在小鼠＂行为绝望＂模型中有一定的抗抑郁作用。     

郁可欣胶囊的抗抑郁作用研究

目的：研究郁可欣胶囊的抗抑郁作用。方法：选择小鼠强迫游泳、悬尾应激及利血平所致眼睑下垂小鼠抑郁模型，用郁可欣胶囊对其进行治疗观察。结果：郁可欣胶囊可以对抗小鼠因强迫悬尾及强迫游泳造成的抑郁症状，并可对抗利血平所致的小鼠眼睑下垂。结论：郁可欣胶囊能明显改善行为绝望动物模型的抑郁症状。     

芍药内酯苷抗抑郁作用的实验研究

目的:研究芍药内酯苷的抗抑郁作用.方法:采用小鼠悬尾、小鼠强迫游泳、小鼠自主活动实验,观察芍药内酯苷对小鼠游泳不动时间、悬尾不动时间及自主活动的影响.结果:芍药内酯苷14、7mg/kg均可显著缩短小鼠悬尾( P＜0.01,P＜0.05)及强迫游泳不动时间(P＜0.05),实验各剂量芍药内酯苷对小鼠自主活动无明显影响.结论:芍药内酯苷具有明显的抗抑郁作用.     

越鞠丸及各单味药醇提物对小鼠的抗抑郁作用研究

目的:探讨越鞠丸全方及各单味药(香附、川芎、栀子、苍术、神曲)醇提物的抗抑郁活性。方法:采用小鼠悬尾和强迫小鼠游泳实验2种行为绝望法复制小鼠抑郁模型,对越鞠丸全方及各单味药分别进行抗抑郁活性研究。结果:除神曲外,越鞠丸全方及各单味药醇提物均能不同程度地缩短小鼠悬尾不动时间和小鼠强迫游泳不动时间,具有抗抑郁样活性;越鞠丸全方醇提物、苍术和川芎可显著缩短小鼠悬尾不动时间和游泳不动时间。结论:越鞠丸全方及香附、苍术、川芎、栀子醇提物均有不同程度的抗抑郁活性,其抗抑郁活性部位/成分可能主要存在于苍术、川芎2味药材之中。     

柴芪口服液抗抑郁作用实验研究

摘要目的：实验观察柴芪口服液的抗抑郁作用。方法：将动物随机分为空白对照组、阳性对照（氟西汀）组、柴芪口服液高、中、低剂量组,分别灌胃给予同等容积的相应药物,qd,连续给予14d,末次给药后40min,采用小鼠悬尾实验、强迫游泳实验及开场实验等方法观察柴芪口服液的抗抑郁作用。结果：在小鼠强迫游泳实验和小鼠悬尾实验中,柴芪口服液大剂量（生药6．4g·kg^-1）能明显缩短两种“行为绝望”模型小鼠的不动时间;在小鼠开场实验中,柴芪口服液6．4g·kg^-1使小鼠水平方向活动次数显著提高,与对照组比较有显著性差异（P＜0．05）。结论：柴芪口服液具有抗抑郁作用。     

花椒多酚类化合物急性给药的抗抑郁作用及机制研究

目的探讨花椒多酚类化合物总提取物（ZPPC）急性给药对ICR小鼠抑郁模型的拮抗作用及可能的机制。方法采用经典的行为绝望抑郁模型,即小鼠悬尾实验和强迫游泳实验,观察ZPPC急性给药对应激小鼠绝望不动时间的影响;同时观察ZPPC对小鼠自主活动的影响,采用荧光分光光度法测定ZPPC对小鼠脑内单胺氧化酶活性的影响。结果与空白组比较,ZPPC（50,100,200mg·kg-1,i.g.）明显缩短小鼠悬尾和强迫游泳实验中的不动时间,其作用类似于阳性对照药丙咪嗪（10mg·kg-1,i.p.）;ZPPC还明显抑制脑内单胺氧化酶的活性。结论 ZPPC急性给药在小鼠行为绝望模型中显示出明显的抗抑郁作用,其作用机制可能与抑制脑内单胺氧化酶活性有关。     

百合地黄汤抗抑郁活性部位的筛选

 目的：筛选百合地黄汤抗抑郁活性部位。方法：选用小鼠行为绝望模型评价百合地黄汤醇提取物及其4个不同极性部位的抗抑郁作用。结果：百合地黄汤醇提取物及其4个不同极性部位均不同程度地缩短绝望模型中小鼠悬尾和强迫游泳的不动时间,其中以百合地黄汤醇提取物(P<0.01)和正丁醇部位(P<0.001)最为显著。结论：百合地黄汤具有抗抑郁作用,活性成分主要分布在正丁醇部位。     

白芍解郁颗粒对小鼠抑郁模型的影响

目的研究白芍解郁颗粒对小鼠抑郁模型的影响. 方法选择小鼠强迫游泳、悬尾应激及利血平所致眼睑下垂小鼠抑郁模型,用白芍解郁颗粒对其进行治疗观察.结果白芍解郁颗粒可以对抗小鼠因强迫悬尾及强迫游泳造成的抑郁症状,并可对抗因利血平所致小鼠眼睑下垂和体温下降.结论白芍解郁颗粒能明显改善抑郁动物模型的抑郁症状.     

绿萼梅提取物对小鼠的抗抑郁作用

目的：观察绿萼梅提取物对小鼠的抗抑郁作用。方法采用悬尾实验（ TFT）、强迫游泳（ FST）等体内药效评价方法观察绿萼梅醇提取物和水提取物对抑郁模型小鼠的治疗作用。结果绿萼梅醇提物能明显缩短小鼠悬尾和强迫游泳不动时间（ P＜0．05）,且对自主活动无影响（ P＞0．05）;而水提物对小鼠悬尾和强迫游泳不动时间无显著影响（ P＞0．05）。结论绿萼梅乙醇提取物具有抗小鼠抑郁作用。     

GL-21抗抑郁的药效初探

目的：初步评价GL-21的抗抑郁药效,并探讨其可能的优势。方法：采用小鼠悬尾试验模型和小鼠强迫游泳试验模型初步评价抗抑郁效果。小鼠自发活动实验评价其兴奋作用。小鼠育亨宾毒性增强实验探讨其可能的作用机制。大鼠阴茎勃起实验,考察其对性功能的改善作用。结果：GL-21（1、2、4、8 mg·kg-1）可显著减少悬尾和强迫游泳试验的不动时间（P<0.01或P<0.05）,且不显著增加小鼠自发活动的总路程。GL-21（2、4、8 mg·kg-1）显著增强育亨宾毒性,增加小鼠死亡。剂量低至1.25 mg·kg-1（sc）可显著诱导大鼠阴茎勃起。结论：GL-21可能具有抗抑郁作用且改善性功能障碍。     

远志醇提物对抑郁症模型小鼠的保护作用研究

目的:研究远志醇提物对抑郁症模型小鼠的保护作用。方法:通过小鼠悬尾实验、强迫游泳实验、慢性不可预知性应激+开场实验分析远志醇提物的抗抑郁作用。各小实验中雄性小鼠均分为模型对照(等容生理盐水)、氯米帕明(20mg·kg-1)和远志醇提物高、中、低剂量(10、5、2.5g·kg-1)组。结果:高、中、低剂量远志醇提物均可缩短抑郁症模型小鼠悬尾不动时间、游泳不动时间和增加移动格数。结论:远志醇提物对模型小鼠抑郁症状态有一定的改善作用。     

淫羊藿苷抗抑郁及对皮质酮致PC12细胞损伤的保护作用研究

目的：研究淫羊藿苷的抗抑郁及对皮质酮致PC12细胞损伤的保护作用。方法：采用大／小鼠强迫游泳、小鼠悬尾三种实验模型,将动物随机分为对照组、淫羊藿苷低剂量组、淫羊藿苷高剂量组、阿米替林组,观察药物对大／小鼠强迫游泳不动时间、小鼠悬尾不动时间的影响;并在细胞水平建立皮质酮损伤PC12细胞模型,观察淫羊藿苷的细胞保护作用。结果：在大／小鼠强迫游泳和小鼠悬尾实验中,淫羊藿苷可显著缩短大／小鼠的强迫游泳不动时间和小鼠悬尾不动时间,与对照组相比差异有统计学意义（P〈0．05）;在皮质酮损伤PC12细胞的模型上,淫羊藿苷可显著提高PC12细胞的存活率,拮抗皮质酮诱导的细胞损伤作用。结论：淫羊藿苷具有明显的抗抑郁效果,其抗抑郁作用与神经细胞保护作用有关。     

Antidepressant-like effect of rutin isolated from the ethanolic extract from Schinus molle L. in mice: evidence for the involvement of the serotonergic and noradrenergic systems

We have recently shown that the hexanic extract from leaves of Schinus molle produces antidepressant-like effects in the tail suspension test in mice. This study investigated the antidepressant-like effect of the ethanolic extract from aerial part of S. molle in the forced swimming test and tail suspension test in mice, two predictive models of depression. Moreover, we investigated the antidepressant potential of rutin, a flavonoid isolated from the ethanolic extract of this plant and the influence of the pretreatment with the inhibitors of serotonin or noradrenaline synthesis, p-chlorophenylalanine methyl ester (PCPA) and alpha-methyl-p-tyrosine (AMPT), respectively in the antidepressant-like effect of this flavonoid. The administration of the ethanolic extract produced a reduction in the immobility time in the tail suspension test (dose range 600-1000 mg/kg, p.o.), but not in the forced swimming test. It also produced a reduction in the ambulation in the open-field test in mice not previously habituated to the arena, but no effect in the locomotor activity in mice previously habituated to the open-field. The administration of rutin reduced the immobility time in the tail suspension test (0.3-3 mg/kg, p.o.), but not in the forced swimming test, without producing alteration in the locomotor activity. In addition, pretreatment of mice with PCPA (100 mg/kg, i.p., for 4 consecutive days) or AMPT (100 mg/kg, i.p.) prevented the anti-immobility effect of rutin (0.3 mg/kg, p.o.) in the tail suspension test. The results firstly indicated the antidepressant-like effect of the ethanolic extract of S. molle in the tail suspension test may be dependent on the presence of rutin that likely exerts its antidepressant-like effect by increasing the availability of serotonin and noradrenaline in the synaptic cleft.     

Group housing of mice increases immobility and antidepressant sensitivity in the forced swim and tail suspension tests

The forced swim test and tail suspension test are often used in laboratory practice to identify compounds that possess antidepressant-like activity. This experiment was conducted to determine whether housing conditions per se influence the response of mice in these antidepressant screening procedures. Male NIH Swiss mice were housed individually or in groups (five per cage) for 8 weeks prior to testing. After 8 weeks, the animals were exposed to the forced swim and tail-suspension tests. Group housed mice displayed high levels of immobility in the forced swim and tail suspension tests. Desipramine injection 60 min prior testing, in doses 7.5 and 15 mg/kg, produced significant reductions in the immobility time in forced swimming and tail suspension tests. Individually housed mice, when exposed to these tests, displayed lower levels of immobility with a magnitude comparable to the effect of desipramine in group housed mice. Desipramine given to individually housed mice did not reduce the duration of immobility either in the forced swim test or in the tail suspension test. These results indicate that both tests are sensitive to housing conditions. This observation suggests that long lasting group housing may be critical to the behavioral response in these preclinical screening procedures in mice.     

Antidepressant-like effect of asiaticoside in mice

In the present study, the potential antidepressant properties of asiaticoside were investigated in male mice in three tests -- splash test in the unpredictable chronic mild stress (CMS) model, tail suspension test (TST), forced swimming test (FST) -- with clomipramine being a positive control. In the splash test, asiaticoside (10 mg/kg, PO) and clomipramine (50 mg/kg, PO) significantly augmented the frequency of grooming behavior in stressed mice. In the tail suspension test, asiaticoside (10, 20 mg/kg, PO) and clomipramine (50 mg/kg, PO) significantly decreased immobility time. In the forced swimming test, asiaticoside (10, 20 mg/kg, PO) and clomipramine (50 mg/kg, PO) significantly decreased immobility time. These results suggest that asiaticoside may have antidepressant-like action.