G蛋白β3基因多态性同维吾尔族人群高血压的关联分析

目的研究G蛋白β3亚单位（G-protein β3 Subunit，GNB3）基因第10外显子C825T多态性同中国维吾尔族人群高血压之间的关联性。方法采用较大样本巢式病例一对照研究（n=738），用聚合酶链式反应（PCR）及限制性片段长度多态性（RFLP）技术对354例高血压患者及384例正常血压者GNB3基因C825T多态进行检测。结果GNB3基因C825T多态3种基因型CC、TT、CT分布频率在病例组为27．2％、42．9％、29．9％．在正常对照组为27．7％、42．4％、29．9％，基因型分布在组间的差异无统计学意义（r=0．0262．P=0．99）。T等位基因分布频率在病例组为51．4％、在正常对照组为51．2％。组间的差异也无统计学意义（x^2=0．0016．P=0．97）。对病例组在不同基因型间进行比较，未发现CC、CT、TT基因型间收缩压、舒张压、体重指数、血糖、血脂水平有统计学差异。结论GNB3 C825CT多态可能与维吾尔族人群高血压无关联。     

内皮型一氧化氮合酶基因G894T和G蛋白β3亚单位基因C825T多态性对高血压的协同作用

目的探讨内皮型一氧化氮合酶(eNOS)基因G894T和G蛋白β3亚单位(GBN3)基因C825T多态性对原发性高血压(EH)的协同作用。方法采用多聚酶链式反应结合限制性内切酶片段长度多态分析方法,检测310例健康人和151例高血压患者的eNOS基因G894T多态性和GBN3基因C825T多态性。结果EH组eNOS G894T多态性的GT+TT基因型和T等位基因频率显著高于对照组(P<0.05);EH组GNB3 C825T多态性中CC、CT、TT基因型频率、C、T等位基因频率与正常对照组比较差异无统计学意义(P>0.05);分析eNOS 894T和GNB3 825T罹患高血压的相对风险,其比数比(OR)为1.77,高于单基因eNOS 894T(0.53)和GNB3 825T(1.05)。结论eNOS基因G894T多态性的T等位基因是中国汉族人EH发病的危险因素之一。eNOS基因894T等位基因和GNB3 825T等位基因对高血压的发生具有协同作用。     

重庆市部分脑卒中病人G蛋白β3亚单位基因多态性研究

目的：观察重庆市脑卒中病人G蛋白β3亚单位基因（GNB3）C825T多态性，探讨脑卒中发生的遗传学机制。方法，急性缺血性脑卒中患者72例，并按性别，年龄配对设对照组，取血标本提取白细胞DNA，用PCR方法扩增目的基因，用限制性内切酶（BseDI）酶切PCR产物用于基因分型，同时观察高血压病（EH）等疾病家族史。结果：脑卒中组GNB3 C825T基因型分布（基因型频率CC＝0．32，CT＝0．58，TT＝0．10）与对照组有显著差别（基因型频率CC＝0．65，CT＝0．32，TT＝0．03，X^2＝14．6，P＜0．01＝，但在脑卒中病人中有或无EH亚组之间基因型分布无差别。Logistic回归分析显示，825T等位基因和EH家族史与脑卒中有关联，825T等痊基因携带者的CC纯合子比较有较高的患脑卒中的风险（OR＝4．17，95％CI1．71－10．15，P＜0．01＝。结论：GNB3基因C825T多态性的T等位基因是重庆市缺血性脑卒中发病的遗传危险因子，遗传造成EH易感性的同时也造成了脑卒中的易感性。     

GNB3基因C825T多态同哈萨克族人群肥胖的关联分析

目的探讨鸟苷酸结合蛋白基因β3亚单位(G-proteinβ3Subunit,GNB3)第10外显子C825T多态同中国哈萨克族人群肥胖的关系。方法应用PCR技术及限制性长度多态(RFLP)技术在277例哈萨克族正常体重者,121例超重者及102例肥胖者中检测GNB3基因C825T多态性。结果GNB3基因C825T多态3种基因型分别为TT、CT及CC。其中CT、TT基因型分布频率在正常体重组、超重组及肥胖组分别为46.9%,54.5%,54.9%,23.4%,16.6%,20.6%,基因型分布在组间无显著性差异(P=0.39)。T等位基因分布在正常体重组、超重组及肥胖组分别为46.9%,43.8%,48.0%,组间也无显著性差异(P=0.63)。将正常体重及超重者按高血压及正常血压分层,发现超重及肥胖的高血压者TT基因型频率(26.2%)较正常体重的高血压者(14.7%)有增高趋势(P=0.061)。结论GNB3基因825T等位基因可能不是中国哈萨克族肥胖的主要遗传易感因子,但TT基因型可能在超重的高血压者中有一定作用,此基因在中国其他人群肥胖合并高血压中的作用有必要进行深入研究。     

怀化侗族高血压高发人群G蛋白β3亚单位基因825C／T多态性研究

目的 探讨G蛋白β3亚单位(GNB3)基因82C/T多态性与怀化侗族高血压高发人群原发性高血压之间的关系。方法 采用聚合酶链反应结合限制性内切酶片段长度多态分析方法(PCR-RFIP)检测96例怀化侗族高血压病人和89例健康人的GNB3825C／T等位基因频率和基因型频率。结果 高血压组CNB3825C／T基因型频率(CC18．8％、CT59．4％、TT21．8％)、等位基因频率(C48．4％、T51．6％)与正常对照组基因型频率(CC24．7％、CT52．8％、TT22．5％)、等位基因频率(C51．1％、T48．9％)比较无显差异；CC基因型患与CT TT型基因型患比较。收缩压和舒张压无显性差异。结论 GNB3基因多态性与怀化侗族人群原发性高血压无关。     

α-adducin基因G460W多态性与脑出血的相关性

目的 探讨α-adducin基因G460W多态性与脑出血的关系。方法 多中心病例对照研究,从7个研究中心收集456例脑出血病例,匹配454例对照,采用聚合酶链反应-限制性片断长度多态(PCR-RFLP)检测α—adducin基因G460W多态性。由心脑血管病标准问卷调查、血生化检查获取脑出血传统危险因素资料。结果 脑出血组和对照组α-adducin基因G460w多态性分布均符合Hardy-Weinberg平衡,脑出血组携带WW基因型(36．8％)和W等位基因个体的频率(60．0％)高于对照组(WW基因型,27.5％;W等位基因,52．0％),差异有显著统计学意义(基因型,P=O．004;等位基因,P=0．000)。多元Logistic回归调整了高血压、过量饮酒和低血清胆固醇等脑出血传统危险因素后,α—adducin基因G460W多态性和脑出血的关联关系依然存在(OR=1．46,95％CI 1．05～2．05)。结论 α-adducin基因G460W多态与脑出血存在关联关系,460w等位基因与脑出血患病危险的增高有关。     

血管内皮生长因子基因多态性与复发性自然流产的关联性研究

目的 探讨血管内皮生长因子（vascular endothelial growth factor,VEGF）基因-2578C/A,-1154G/A,-460T/C和＋936C/T 4个多态性位点与复发性自然流产（recurrent spontaneous abortion,RSA）的关联性.方法 分别采用聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）法和等位基因特异性扩增-聚合酶链反应法（AS-PCR）,检测203名患者和190名健康妇女-2578C/A、-1154G/A、-460T/C和＋936C/T多态位点基因型及等位基因频率的分布情况.结果 -2578 C/A和-1154G/A多态位点的基因型频率、等位基因频率在病例组和对照组间差异均无统计学意义（P＞0.05）.-460T/C和＋936C/T位点基因型频率等位基因频率,病例组和对照组相比均有统计学差异（P＜0.01）;VEGF-2578C/A、-460T/C、＋936C/T多态位点的单倍型频率在病例组和对照组差异有统计学意义（P＜0.01）.结论 携带VEGF-460TT基因型及＋936T等位基因的基因型可能会增加女性复发性自然流产的发病风险,单倍型VEGF-2578/-460/＋ 936CTT、ATT、CCC和ACC与复发性自然流产的发病可能相关.     

G蛋白β_3基因多态性与朝鲜族原发性高血压的相关性分析

[目的]探讨G蛋白β3亚单位(GNB3)基因G814A,C825T位点多态性与延边朝鲜族原发性高血压(EH)之间的相关性.[方法]应用多重单碱基延伸分型技术对延边朝鲜族223例EH患者和244例血压正常者GNB3基因的G814A,C825T位点的多态性进行检测.[结果]467个被检测样本中,G814A位点的基因型均为GG型;C825T位点CC,CT,TT基因型频率在EH组和对照组中分别为34.1%,43.9%,22.0%和26.6%,53.7%,19.7%,T等位基因频率在EH组和对照组中分别为43.9%和46.3%,差异均无统计学意义(P>0.05);在EH组中不同基因型生理生物化学指标间差异亦无统计学意义(P>0.05).[结论]GNB3基因G814A位点在延边朝鲜族中不存在多态性;GNB3基因C825T位点的多态性与延边朝鲜族EH的发生可能无相关性.     

G蛋白β3亚单位基因C825T多态性与高血压发病的关系

目的　观察高血压病 (EH)病人G蛋白 β3亚单位基因 (GNB3 )C82 5T多态性 ,探讨EH发生的遗传学机制。 方法　EH病人 112例 ,设对照组。取血标本提取DNA ,用PCR方法扩增目的基因 ,用限制性内切酶 (BseDⅠ )酶切PCR产物用于基因分型 ,同时观察血脂、体质量指数 (BMI)、EH家族史。结果　EH病人GNB3C82 5T基因型分布 (基因型频率CC =0 .3 4,CT =0 .5 3 ,TT =0 .13 )与对照组有显著差别 (基因型频率CC =0 .5 9,CT =0 .3 6,TT =0 .0 5。 χ2 =6.9,P <0 0 5 ) ;Logis tic回归分析显示 ,C82 5T等位基因与EH关联最密切 (OR =2 .2 ,95 %CI 1.1～ 4.6)。结论　GNB3基因C82 5T多态性的T等位基因是EH发病的遗传危险因子。     

G蛋白β3亚单位C825T多态性与房颤的关系

目的探讨在中国人群中,G蛋白β3亚单位基因C825T多态位点与家族性房颤及散发性房颤之间的关系.方法利用PCR-RFLP技术对20例家族性房颤患者（无亲缘关系的20个家系,每个家系中随机抽取1例患者）,93例散发性房颤患者及284例正常对照组进行基因型分析.结果（1）家族性房颤组TT基因型频率和T等位基因频率（40%和60%）均明显高于正常对照组（15.8%和42%）（P＜0.05）;（2）散发性房颤组中TT基因型频率和T等位基因频率（19.4%和46%）略高于正常对照组（15.8%和42%）,但未达统计学显著意义（P＞0.05）.结论G蛋白β3亚单位基因C825T多态性与中国人群家族性房颤相关,825T等位基因可能是家族性房颤的遗传危险因素.     

Influence of G-protein β-Polypeptide 3 C825T Polymorphism on Antihypertensive Response to Telmisartan and Amlodipine in Chinese Patients

Background:

G-protein β-polypeptide 3 (GNB3) is a β subunit isoform of G-protein that plays important role in signal transduction of membrane G-protein coupled receptors (GPCRs). The GNB3 splice variant C825T (rs5443) is associated with risk for essential hypertension (EH) and efficacy of therapeutic drugs targeting GPCRs. It is unknown whether the polymorphism is associated with blood pressure (BP) response to telmisartan or amlodipine, two widely prescribed antihypertensive drugs.

Methods:

A total of 93 subjects initially diagnosed as EH were recruited and underwent a 4-week treatment with telmisartan (42 patients) or amlodipine (51 patients) monotherapy. Both baseline and after-treatment BP were measured. GNB3 C825T polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism.

Results:

Baseline systolic BP (SBP) and diastolic BP (DBP) were comparable among C825T genotypes in both telmisartan and amlodipine treatment groups. Patients with the CT or TT genotypes showed significantly lower body mass index (BMI) as compared with CC homozygotes in both groups (P < 0.05, respectively). GNB3 825TT homozygotes showed significantly higher after-treatment DBP and mean arterial pressure (MAP) than those carrying at least one 825C allele (P < 0.01) in the telmisartan treatment group. No difference in after-treatment SBP, DBP, and MAP levels among C825T genotypes was observed in the amlodipine treatment group. No significant difference in absolute changes in BP levels was observed among the genotypes in either treatment group.

Conclusion:

The GNB3 C825T splice variant is associated with the DBP-lowering effect of telmisartan but not amlodipine in Chinese EH patients.     

G蛋白β3亚单位基因多态性与抗精神病药源性肥胖的相关性

目的 探讨G蛋白β3亚单位(GNB3)基因C825T多态性与抗精神病药源性肥胖的关系.方法 收集126例长期应用抗精神病药的精神分裂症患者,按体重指数(BMI)分成肥胖组(62例)和非肥胖组(64例);采用聚合酶链式反应(PCR)和DNA测序技术测定GNB3基因C825T多态性;常规检测空腹血糖(FBG)、餐后2h血糖(2hPBG)、血脂、血尿酸(UA)水平.结果 (1)肥胖组与非肥胖组均发现GNB3基因C825T多态性,并符合Hardy-Weinberg平衡率;(2)肥胖组基因型频率(CC 17.75％,CT 58.06％,TT24.19％)与非肥胖组(CC 18.75％,CT 62.50％,TT 18.75％)比较差异无统计学意义(x2=0.59,P＞0.05),肥胖组等位基因频率(C 46.77％,T 53.23％)与非肥胖组(C 50％,T 50％)比较差异也无统计学意义(x2=0.26,P＞0.05);(3)不同基因型之间BMI、FBG、2hPBG、血脂、UA水平差异无统计学意义(均P＞0.05),携T等位基因(CT型+TT型)与非携T等位基因者(CC型)之间BMI、FBG、2hPBG、血脂、UA水平差异也无统计学意义(均P＞0.05).结论 GNB3基因C825T多态性可能不是抗精神病药源性肥胖的基因危险因素.     

GNB3 C825T and ACE I/D polymorphisms on the sodium-proton exchanger and the prevalence of essential hypertension in males

BACKGROUND: The aim of the study was to verify the hypothesis if the interaction between the G protein beta3 subunit (GNB3) C825T polymorphism and ACE I/D polymorphism could lead to the disclosure of increased activity of sodium-proton exchanger and hypertension. METHODS: The study included 44 male patients, median age: 40 years. Patients were divided into two groups: 26 patients with essential hypertension (EH), and 18 subjects in the normotensive group (C). RESULTS: CT + TT genotypes of GNB3 predominated in patients with hypertension (65%) compared to normotensive patients (12%) (p <0.01). No significant differences were observed in the frequency of ACE gene polymorphisms between the examined groups. Significantly higher activity of erythrocyte NHE in patients with EH was observed: median 8.83 (interquartile range 4.27) mmol/l RBC/h, compared to C: median 6.18 (2.80) mmol/l RBC/h, p <0.001. Multiple logistic regression analysis showed that the presence of the T allele increased the risk of hypertension 16-fold (p <0.01) and higher erythrocyte NHE activity 2-fold per each unit of activity (p <0.01). DD genotype of ACE polymorphism did not increase the risk of hypertension. No significant interaction of the influence of GNB3 T allele and ACE DD genotype on the risk of hypertension was observed. In multiple linear regression analysis, none of the examined genotypes and their interactions influenced NHE activity. CONCLUSIONS: The presence of the T allele of GNB3 polymorphism and increased activity of erythrocyte NHE independently of ACE genotype increase the risk of hypertension.     

酪氨酸羟化酶基因C-824T多态性与湖南汉族人群 原发性高血压易感性的关联研究

目的：通过病例-对照研究,探讨酪氨酸羟化酶（tyrosine hydroxylase, TH）基因C-824T多态性与中国湖南汉族人群原发性高血压遗传易感性的关系。方法：应用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)的分析方法,在368例原发性高血压患者和353例健康人群中对TH基因的C-824T进行基因分型。结果：(1)高血压病例组TH基因C-824T位点CC和CT+TT基因型频率分别为89.9%和10.1%,对照组CC和CT+TT基因型频率分别为88.7%和11.3%,2组间基因型的分布差异无统计学意义(P=0.579);高血压病例组和对照组中C等位基因的频率分别为94.8%和94.1%,两者之间差异亦无统计学意义（P=0.515）。(2)经Logistic回归分析结果显示,TH基因C-824T多态性与高血压病的发病风险无关（P=0.264）。(3)通过对性别进行分层分析,男性及女性病例组与对照组间TH基因C-824T基因型分布均无统计学差异(P=0.841及P=0.288)。(4)对照组TH基因C-824T多态位点CT+TT基因型个体舒张压显著高于基因型为CC的个体（P=0.015）。(5)通过对性别进行分层分析,对照组男性CT+TT基因型个体舒张压显著高于基因型为CC的个体（P=0.018）;对照组女性CT+TT基因型与CC基因型个体的舒张压的差别没有统计学意义（P=0.083）。结论：TH基因C-824T的遗传多态性与湖南汉族人群原发性高血压的易感性无关;TH基因C-824T的遗传多态性与湖南汉族人群男性的舒张压水平有关。     

G蛋白β3亚单位基因C825T多态性与2型糖尿病合并大血管病变的关系

目的探讨合肥地区汉族人群G蛋白β3亚单位基因C825T多态性（GNβ3C825T）与2型糖尿病（T2DM）合并大血管病变（MA）的遗传易感性。方法采用聚合酶链反应结合限制性内切酶片段长度多态分析方法（PCR-RFLP）检测293例合肥地区汉族T2DM患者GNβ3C825T多态性,并测定T2DM患者的体质指数（BMI）、腰臀比（WHR）、收缩压（SBP）、舒张压（DBP）、空腹血糖（FBG）、餐后2 h血糖（P2hPG）、胰岛素敏感指数（ISI）等指标。结果 T2DM无MA组GNβ3C825T中基因型频率（CC为30.6%,CT为50.0%,TT为19.4%）、等位基因频率（C为55.6%,T为44.4%）与T2DM有MA组基因型频率（CC为29.3%,CT为48.8%,TT为21.9%）、等位基因频率（C为53.7%,T为46.3%）分布差异无统计学意义,在调整年龄、性别、吸烟、高血脂、高血压后,Logistic回归分析显示在糖尿病人群中MA与GNβ3C825T无关。结论合肥地区汉族人群中GNβ3C825T多态性可能与T2DM合并MA遗传易感性无关。     

E选择素基因C602A和T1559C多态性与原发性高血压的相关性

目的 探讨E选择素基因单核苷酸多态性与原发性高血压的相关性.方法 筛选健康对照人群500人(正常血压组)和高血压患者930例,提取血液白细胞基因组DNA后设计特定的引物,通过荧光定量聚合酶链反应确定E选择素基因的C602A和T1559C二个单核苷酸位点的多态性.结果 正常血压组和高血压组的C602A基因型频率差异有统计学意义(P<0.01).CC、CA、AA基因型分别为26(5.2%)、20(4.O%)、454(90.8%)例和14(1.5%)、53(5.7%)、863(92.8%.)例.C等位基因频率之间的差异有统计学意义(P<0.01).C、A频率分别占7.2%、92.8%和4.4%、95.6%.男性正常血压组和高血压组的基因型频率差异有统计学意义(P<0.05).CC、CA、AA基因型分别为14(4.7%)、11(3.7%)、272(91.6%)例和10(1.7%)、34(5.8%)、545(92.5%)例.女性除CC+CA外其他基因型频率差异均有统计学意义(均P<0.01).正常血压组和高血压组T1559C的TT、TC、CC基因型频率分别为57(11.4%)、200(40.0%)、43(48.6%)例和66(7.1%)、354(38.1%)、510(54.8%)例;T、C频率分别占31.4%、68.6%和26.1%、73.9%,差异均有统计学意义(均P<0.01).男性正常血压组和高血压组的TT、TC、CC频率分别为36(5.9%)、117(39.4%)、144(48.5%)和35(5.9%)、230(39.0%)、354(55.0%);等位基因T、C的频率分别占31.4%、68.6%和26.1%、73.9%,差异均有统计学意义(均P<0.01).女性的T1559C基因型和等位基因频率分布与血压无明显相关.结论 C602A和T1559C多态性与原发性高血压之间存在着明显的相关性.性别分组后,C602A与男、女性均相关,T1559C仅与男性高血压患者相关.

Abstract：

Objective To investigate the possible genetic associations between the C602A and T1559C polymorphisms of E-selectin(SELE)and essential hypertension.Methods Essential hypertensive patients(n=500)and heathy normotensive subjects(n=930)were screened for the genotypes C602A and TI559C by real-time quantitative polymerase chain reaction after DNA extraction to identify representative variations in the SELE gene.Results Normotensive subjects and hypertensive patients were significantly different with respect to the genotypes CC,CA and AA,26(5.2%),20(4.0%)and 454(90.8%)vs 14(1.5%),53(5.7%)and 863(92.8%)respectively of C602A.And the C-allele frequency was also signiflcantlv different between the NT and EH groups(C,A=7.2%,92.8%vs 4.4%,95.6%).When subgrouped by gender,frequency of CC,CA,AA between normotensive and essential hypertensive males was 14(4.7%、),11(3.7%),272(91.6%)and 10(1.7%),34(5.8%),545(92.5%),which differed significantly(P<0.05),while in female groups,all the frequency of genotypes were significantly different (P<0.01)except CC+CA.The additive model(TT,TC,CC)of the T1559C genotype was significantly different between essential hypertensive and normotensive groups overall,57(11.4%),200(40.0%),43(48.6%)and 66(7.1%),354(38.1%),510(54.8%),respectively.The T-allele of hypertensive patients significantly difiered from normoteasive subjects(T,C=31.4%,68.6%vs 26.1%,73.9%respectively).When subgrouped by gender,between the male NT and EH groups,the TT,TC and CC frequency of Tl559C were 36(5.9%),117(39.4%),144(48.5%)and 35(5.9%),230(39.O%),354(55.0%),and the frequency of T vs C was31.4%vs 68.6%and 26.1%vs 73.9%,which were significantly different(all P<0.01).As in female NT and EH groups,there were not significant differences existed at all.Conclusion C602A and T1559C of SELE are associated with essential hypertension in the Chinese population.and T1559C is closely related with male hypertension other than in females.     

Beta2-adrenoceptor gene variant Arg16Gly is associated with idiopathic ventricular outflow-tract tachycardia

Background Imbalance of the sympathetic nervous system was involved in the pathogenesis of idiopathic ventricular outflow-tract tachycardia （IVOT）. We aimed to investigate whether the major genetic variants in β1-and β2-adrenoceptors and GNB3 C825T were associated with IVOT and verapamil sensitive idiopathic left ventricular tachycardia （ILVT）.Methods Patients with IVOT and ILVT from December 2005 to December 2007 were consecutively enrolled into this study. Controls were randomly selected from the community-based inhabitants. Five genetic variants, Ser49Gly and Gly389Arg in the β1-adrenoceptor, Arg16Gly and Gln27Glu in the β2-adrenoceptor and GNB3 C825T, were genotyped by polymerase chain reaction-restriction fragment length polymorphism analysis.Results A total of 227 patients with IVOT and 110 patients with ILVT were included. Genotyping revealed that the 16Gly allele of Arg16Gly variant of β2-adrenoceptor was associated with a higher risk of IVOT （OR：1.40, 95% CI： 1.12-1.75,P=0.003 in the addictive model and OR：. 1.62, 95% CI： 1.14-2.31, P=0.007 in the dominant model）. Patients with Gly16Gln27 haplotype also had a higher risk of IVOT （OR： 1.38, 95% CI： 1.11-1.73, P=0.012）. Other four variants,including Ser49Gly and Arg389Gly in β1-adrenoceptor, GIn27Glu in β2-adrenoceptor and GNB3 C825T, did not differ between patients with IVOT and controls. In patients with ILVT, no significant difference was found in these five variants compared with controls.Conclusions Arg16Gly in β2-adrenoceptor is significantly associated with IVOT in Chinese Han population. Major genetic variants in β1- and β2-adrenoceptor and GNB3 C825T may not be associated with ILVT. These data suggest a different arrhythmogenic mechanism in IVOT and ILVT.     

G蛋白β3亚单位C825T与高血压、胰岛素抵抗及肥胖的关联

目的　分析GNB3 基因C82 5T多态位点与原发性高血压、胰岛素抵抗和肥胖的关系。方法　对大庆地区 187个高血压家系和 189个非高血压家系的第一代子女进行空腹血糖、空腹胰岛素、血脂和纤维蛋白原等指标的测定 ,用聚合酶链反应 (PCR)扩增和酶切方法检测GNB3 C82 5T多态性 ,以多因素分析探讨各基因型与血压、胰岛素抵抗的关系。结果　 (1)高血压家族史阳性组CT/TT基因型频率高于高血压家族史阴性组 (0 78∶0 6 9,P =0 0 6 )。 (2 )调整年龄、性别影响后 ,CT/TT基因型胰岛素敏感性显著低于CC基因型 ,血压、体重指数显著高于CC基因型。 (3)相关分析显示 ,CT和TT基因型组中 ,胰岛素敏感性与收缩压水平负相关 (r=- 0 35 19,P =0 0 0 0 1) ,CC基因型组则相关不显著 (r=- 0 0 0 5 5 ,P =0 93)。按胰岛素敏感指数中值将其分为两组后 ,胰岛素敏感性较差组中 ,CT和TT基因型者收缩压明显高于CC基因型者 (14 6mmHg± 1 84mmHgvs 132mmHg±5 19mmHg ,P <0 0 5 ) ;胰岛素敏感性较好组中 ,两者血压无显著差异。 (4 )无高血压家族史的第一代子女 ,CT和TT基因型组胰岛素敏感性也与收缩压呈负相关 (r=- 0 4 86 4 ,P =0 0 0 1) ,CC基因型组二者相关不显著。结论　GNB3 C82 5T基因型与胰岛素敏感性、血压水平及体