慢性病毒性肝炎G、S分期与胆囊声像图的关系

目的探讨慢肝病毒性肝炎(慢肝)G、S分期与胆囊声像图变化的关系.方法363例慢性病毒性肝炎患者根据肝穿刺活检组织学结果分为无肝纤维化(S0)到肝硬化(S4)5期,按炎症分级为G1～G4 4级.104例正常对照纽为无任何肝病及胆囊疾病的体检健康者.比较慢肝组与对照组之间以及各G/S分期之间胆囊声像图的差异.结果慢肝组与对照组之间GBW、GBA、GBT差异有显著性且慢肝组均大于对照组.8例胆囊壁呈双边的均出现在慢肝组;随G/S分期增高胆囊逐渐增大,胆囊呈双边的病例(G/S1～3期内)也增多.炎症活动度G分度越高,胆囊越大,壁越厚.结论慢性病毒性肝炎胆囊声像图异常的程度与慢肝G、S分期、病程长短及慢肝严重程度有关.胆囊声像图的动态观察对评价慢肝病情有一定价值.     

240例肝穿刺活检的病理诊断与B超显像比较分析

目的:探讨B型超声显像在慢性肝炎分级分期中的作用,寻求超声检查方法对慢性乙型肝炎患者病理分级分期的诊断标准.方法:对240例慢性乙型肝炎患者B超表现与肝组织病理分级分期进行对比分析.结果:超声诊断慢性乙型病毒性肝炎的声像图征象如肝脏的包膜、实质回声、血管走形和胆囊壁的改变及门静脉、脾静脉、脾脏厚度和慢性乙型肝炎肝组织炎症活动程度显著相关(P<0.05);结论B超的声像图在评价慢性病毒性肝炎病情的轻重有重要的价值.     

病毒性肝炎病理分期与脾脏指标的相关性研究

目的探讨慢性病毒性肝炎病理分期与脾脏大小及脾静脉指标的关系。方法 234例慢肝患者根据肝穿结果肝纤维化程度分为S0～S4五期,按炎症分为G1～G4四期。比较慢肝不同病理分期之间脾脏指标的差异。结果 S分期之间脾脏大小及脾静脉内径差异有统计学意义,且随S分期增高而增大。G4与其它各分期之间脾脏大小、脾静脉内径差异有统计学意义,且高于其它各组。结论慢肝患者脾脏增大,脾静脉内径增宽随肝脏病理纤维化及炎症分期的增高而增大。     

胆囊壁厚度与病毒性肝炎关系初探

目的:探讨胆囊壁厚度及双边征与病毒性肝炎的内在联系。方法:通过超声检查观察病毒性肝炎和慢性胆囊炎的胆囊壁厚度。结果:病毒性肝炎的胆囊壁厚度异常率明显高于慢性胆囊炎。结论:胆囊壁厚度明显异常( 包括胆囊壁厚度≥5 m m 及双边征) 为病毒性肝炎的一种表现形式,它的发生是肝包膜和胆囊壁的双重炎性反应。胆囊壁厚度异常人数与病毒性肝炎病程成正比     

慢性病毒性肝炎肝纤维化分期与门、脾静脉血流的关系

目的 探讨慢性病毒性肝炎不同G、S分期与门、脾静脉血流动力学变化的关系.方法根据肝穿刺活检结果将348例慢性病毒性肝炎患者分为无肝纤维化(S0)～肝硬化(S4)5期,按炎症分级为G1～G4 4级.比较慢肝患者不同G/S分期之间门、脾静脉血流的差异.结果不同G分期之间Drpv、RPVFV差异有显著性,且随分期增高值明显增大.不同S分期之间Dpv、Drpv、Dspv、VsSPV、VmSPV差异有显著性.S1～3分期内Dpv、Drpv随S分期增高而增宽;S1～4分期内Dspv随S分期增高而增宽;S0～4分期内VsSPV、VmSPV随S分期增高而降低.慢肝不同程度之间Drpv、Dspv、RPVFV差异有显著性,且随慢肝程度越重数值越大.VsPV与VsRPV、VsLPV、VsSPV的相关系数r分别为0.496、0.377、0.442.PVFV与RPVFV、LPVFV、SPVFV的相关系数r分别为0.482、0.334、0.370.结论门、脾静脉血流动力学改变与慢性肝炎不同G、S分期相关.动态测定门、脾静脉速度及血流量可以反映慢肝患者肝纤维化的程度.     

肿瘤坏死因子α在慢性病毒性肝炎中的作用

目的　探讨TNF -α在慢性病毒性肝炎炎症活动及纤维化形成中的作用。方法　将 83例慢性病毒性肝炎及肝硬化患者肝穿取得的肝组织分别按炎症活动度分级和纤维化程度分期 ,应用ELISA方法检测患者血清TNF -α水平。并计算肝组织炎症活动度计分及纤维化程度计分。结果　血清TNF -α水平在肝组织炎症活动度G2 、G3及G4 级比正常对照组明显升高 (P <0 0 0 1) ,且随着肝组织炎症活动程度的加重而升高。血清TNF -α水平与肝组织炎症活动度计分呈低度正相关 (P <0 0 1)。血清TNF -α水平在肝纤维化S2 、S3及S4 期比正常对照组明显升高 (P <0 0 5) ,且随着肝纤维化程度的加重而不断升高。血清TNF -α水平与肝组织纤维化程度计分呈中度的正相关 (P <0 0 0 1)。结论　TNF -α在慢性病毒性肝炎中 ,既参与了肝组织炎症活动 ,也参与了肝纤维化的形成。     

慢性病毒性肝炎肝纤维化分期与门、脾静脉血流的关系

目的：探讨慢性病毒性肝炎不同G、S分期与门、脾静脉血流动力学变化的关系。方法：根据肝穿刺活检结果将348例慢性病毒性肝炎患者分为无肝纤维化（S0）～肝硬化（S4）5期,按炎症分级为G1～G44级。比较慢肝患者不同G/S分期之间门、脾静脉血流的差异。结果：不同G分期之间Drpv、RPVFV差异有显著性,且随分期增高值明显增大。不同S分期之间Dpv、Drpv、Dspv、VsSPV、VmSPV差异有显著性。S1～3分期内Dpv、Drpv随S分期增高而增宽;S1～4分期内Dspv随S分期增高而增宽;S0～4分期内VsSPV、VmSPV随S分期增高而降低。慢肝不同程度之间Drpv、Dspv、RPVFV差异有显著性,且随慢肝程度越重数值越大。VsPV与VsRPV、VsLPV、VsSPV的相关系数r分别为0.496、0.377、0.442。PVFV与RPVFV、LPVFV、SPVFV的相关系数r分别为0.482、0.334、0.370。结论：门、脾静脉血流动力学改变与慢性肝炎不同G、S分期相关。动态测定门、脾静脉速度及血流量可以反映慢肝患者肝纤维化的程度。     

病毒性肝炎的胆囊声像图改变

病毒性肝炎的胆囊声像图改变承钢职工医院宋钰卿，刘志茹采用日本ＡｌｏｋａＳＳＤ─２８０型线阵超声诊断仪，对１９３例病毒性肝炎患者进行胆囊声像图检查。结果：胆囊异常者１３８例，异常率７１．５％。主要改变为胆囊壁增厚或双边征，表明胆囊病变为病毒性肝炎的常见...     

病毒性肝炎患者胆囊改变的超声观察与临床分析

本文对142例病毒性肝炎患者的胆囊壁进行超声观察,旨在探讨胆囊壁声像图表现与病毒性肝炎之间的关系.     

胆囊壁厚度与病毒性为关系初探

目的：探讨胆囊壁厚度及双边征与与病毒性肝炎的内在联系。方法；通过超声检查病毒性为和慢性胆囊炎的胆囊壁厚度。病毒性肝炎的胆囊壁厚度异常率明显高于慢性胆囊炎。结论：胆囊壁厚度明显异常（包括胆囊壁厚度≥５ｍｍ及双边征）为病毒性肝炎的一种表现形式，它的发生是肝包膜和胆囊壁的双重炎性反应。胆囊壁厚度异常人数与病毒性肝炎病程成正比。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.