Effects of 24 Chinese Medicinal Herbs on Nucleic Acid, Protein and Cell Cycle of Human Lung Adenocarcinoma Cells

Ｅｆｆｅｃｔｓ　ｏｆ　２４　Ｃｈｉｎｅｓｅ　Ｍｅｄｉｃｉｎａｌ　Ｈｅｒｂｓ　ｏｎ　Ｎｕｃｌｅｉｃ　Ａｃｉｄ，　Ｐｒｏｔｅｉｎ　ａｎｄ　Ｃｅｌｌ　Ｃｙｃｌｅ　ｏｆ　Ｈｕｍａｎ　Ｌｕｎｇ　Ａｄｅｎｏｃａｒｃｉｎｏｍａ　ＣｅｌｌｓＨＡＮＭｉｎｇ－ｑｕａｎ，...     

Effect of Astragalin on matrix secretion and β1 integrin mRNA expression in human mesangial cells

Ｍｅｓａｎｇｉａｌｃｅｌｌｓ(ＭＣ)ｐｒｏｌｉｆｅｒａｔｉｏｎａｎｄａｃｃｕｍｕｌａｔｉｏｎｏｆｅｘｔｒａｃｅｌｌｕｌａｒｍａｔｒｉｘ(ＥＣＭ)ｐｌａｙａｋｅｙｒｏｌｅｉｎｐｒｏｇｒｅｓｓｉｏｎｏｆｃｈｒｏｎｉｃｒｅｎａｌｄｉｓｅａｓｅｓ1　Ｒｅｃｅｎｔｓｔｕｄｉｅｓ2　ｈａｖｅｓｈｏｗｎｔｈａｔβ1ｉｎｔｅｇｒｉｎｉｎｖｏｌｖｅｓｉｎｔｈｅｐａｔｈｏｇｅｎｅｓｉｓｏｆｇｌｏｍｅｒｕｌｏｓｃｌｅｒｏｓｉｓｂｙｍｏｄｕｌａｔｉｏｎｏｆＭＣｐｒｏｌｉｆｅｒａｔｉｏｎａｎｄＥＣＭｓｅｃｒｅｔｉｏｎＴｈｅｓｕ…     

Effects of Multiglycoside of Tripterygium Wilfordii(GTW)on Spermatogenic Ce

ＥｆｆｅｃｔｓｏｆＭｕｌｔｉｇｌｙｃｏｓｉｄｅｏｆＴｒｉｐｔｅｒｙｇｉｕｍＷｉｌｆｏｒｄｉｉ（ＧＴＷ）ｏｎＳｐｅｒｍａｔｏｇｅｎｉｃＣｅｌｌｓａｎｄ　ＥｎｚｙｍａｔｉｃＡｃｔｉｖｉｔｉｅｓｉｎ　ＭａｌｅＲａｔｓＴｉａｎＪｉａｎａｎｄＺｈｏｕＸｉａｏ－...     

SEM observation on LAK cells killing the human rectal adenocarcinoma cells in vitro

ＳＥＭｏｂｓｅｒｖａｔｉｏｎｏｎＬＡＫｃｅｌｌｓｋｉｌｌｉｎｇｔｈｅｈｕｍａｎｒｅｃｔａｌａｄｅｎｏｃａｒｃｉｎｏｍａｃｅｌｌｓｉｎｖｉｔｒｏＤｉｎｇＹａｎｑｉｎｇ（丁彦青）；ＬｉＣｈｕｎｄｅ（李春德）；ＺｈａｎｇＪｉｕｈｕａ（张进华）；ｚｈｕＭｅｉ...     

Phenotype Analysis of Mononuclear Cells in Endomyocardial Biopsy Specimens from Patients with Viral Myocarditis and Dilated C

ＰｈｅｎｏｔｙｐｅＡｎａｌｙｓｉｓｏｆＭｏｎｏｎｕｃｌｅａｒＣｅｌｌｓｉｎＥｎｄｏｍｙｏｃａｒｄｉａｌＢｉｏｐｓｙＳｐｅｃｉｍｅｎｓｆｒｏｍＰａｔｉｅｎｔｓｗｉｔｈＶｉｒａｌＭｙｏｃａｒｄｉｔｉｓａｎｄＤｉｌａｔｅｄＣａｒｄｉｏｍｙｏｐａｔｈｙＬＩＷ...     

TEM observation on LAK cells killing the human rectal adenocarcinoma cells in vitro

ＴＥＭｏｂｓｅｒｖａｔｉｏｎｏｎＬＡＫｃｅｌｌｓｋｉｌｌｉｎｇｔｈｅｈｕｍａｎｒｅｃｔａｌａｄｅｎｏｃａｒｃｉｎｏｍａｃｅｌｌｓｉｎｖｉｔｒｏ￥ＤｉｎｇＹａｎｑｉｎｇ（丁彦青），ＬｉＣｈｕｎｄｅ（李春德），ＺｈａｎｇＪｉｎｈｕａ（张进华），ＺｈｕＭｅ...     

Study of the Effects of the Chinese Herbal Prescription Combined with Copper and Ferum on the Malignancy of C

ＳｔｕｄｙｏｆｔｈｅＥｆｆｅｃｔｓｏｆｔｈｅＣｈｉｎｅｓｅＨｅｒｂａｌＰｒｅｓｃｒｉｐｔｉｏｎＣｏｍｂｉｎｅｄｗｉｔｈＣｏｐｐｅｒａｎｄＦｅｒｕｍｏｎｔｈｅＭａｌｉｇｎａｎｃｙｏｔＣａｎｃｅｒＣｅｌｌｓＣＨＩＹｏｎｇ－ｃｈｕｎ（迟永春）；ＷａｎＦａｎ...     

StudyonMitochondrialUltrastructure，TracoElementsandTheirCorrelativeFactoreinCellsotGastricMucosaottheGastropathicPatientawith

ＳｔｕｄｙｏｎＭｉｔｏｃｈｏｎｄｒｉａｌＵｌｔｒａｓｔｒｕｃｔｕｒｅ，ＴｒａｃｏＥｌｅｍｅｎｔｓａｎｄＴｈｅｉｒＣｏｒｒｅｌａｔｉｖｅＦａｃｔｏｒｅｉｎＣｅｌｌｓｏｔＧａｓｔｒｉｃＭｕｃｏｓａｏｔｔｈｅＧａｓｔｒｏｐａｔｈｉｃＰａｔｉｅｎｔａｗｉｔｈ...     

Effect of Astragalus Membranaceus on Ca￣(2＋）Influx and CVB3 RNA Replication in Cultured Neonatal Rat Heart C

ＥｆｆｅｃｔｏｆＡｓｔｒａｇａｌｕｓＭｅｍｂｒａｎａｃｅｕｓｏｎＣａ￣（２＋）ＩｎｆｌｕｘａｎｄＣＶＢ３ＲＮＡＲｅｐｌｉｃａｔｉｏｎｉｎＣｕｌｔｕｒｅｄＮｅｏｎａｔａｌＲａｔＨｅａｒｔＣｅｌｌｓＩｎｆｅｃｔｅｄｗｉｔｈＣＶＢ３ＧＵＯＱｉ（郭棋）；ＰＥ...     

Effects of salvia miltiorrhiza bung （SMB） onosteoblast－like cell lin （clonal MC3T3－E1 cells）

Ｅｆｆｅｃｔｓｏｆｓａｌｖｉａｍｉｌｔｉｏｒｒｈｉｚａｂｕｎｇ（ＳＭＢ）ｏｎｏｓｔｅｏｂｌａｓｔ－ｌｉｋｅｃｅｌｌｌｉｎ（ｃｌｏｎａｌＭＣ３Ｔ３－Ｅ１ｃｅｌｌｓ）￥（丁寅）ＤｉｎｇＹｉｎ；ＳｕｉｃｈＳｏｍａ；Ｔ．ＴａｋａｎｏＹａｍａｍｏｔｏ；Ｓ．Ｍａ...     

肺、脑动脉内皮和平滑肌细胞急性缺氧时某些第二信使的变化

目的研究急性缺氧时细胞内第二信使系统的变化。方法培养猪肺、脑动脉内皮细胞与平滑肌细胞,测定在急性缺氧时胞浆游离钙、磷酸肌醇和环核苷酸的变化。结果肺动脉内皮细胞缺氧时有Ｃａ内流增加、ＩＰ３和ｃＡＭＰ无明显变化,ｃＧＭＰ于缺氧４ｍｉｎ升至高峰,随即回降;而脑动脉内皮细胞缺氧时不仅有Ｃａ内流,且有内钙释放、ＩＰ３和ｃＡＭＰ增多,ｃＧＭＰ浓度在缺氧８ｍｉｎ时仍继续上升。肺与脑动脉平滑肌细胞间缺氧时第二信使的变化,仅有ｃＧＭＰ不同。结论内皮细胞的异质性可能与两血管反应性差异有关。     

ATP Evokes Calcium Transients in Single Pulmonary Artery Endothelial Cell in

（胡清华）（王迪浔）（张宇）（陈荣三）（王金唏）ＡＴＰＥｖｏｋｅｓＣａｌｃｉｕｍＴｒａｎｓｉｅｎｔｓｉｎＳｉｎｇｌｅＰｕｌｍｏｎａｒｙＡｒｔｅｒｙＥｎｄｏｔｈｅｌｉａｌＣｅｌｌｉｎＰｒｉｍａｒｙＣｕｌｔｕｒｅｓ￥ＨＵＱｉｎｇ－ｈｕａ，ＷＡＮＧＤｉ－...     

Albumin permeability across endothelial cell monolayer exposed to reactive oxygen intermediates: involvement of reversible functional alteration of the cell membrane Ca2+ channels

This study was designed to test the idea that the redox state of sulfhydryl (SH)-groups in cell-membrane Ca2+ channels plays a pivotal role in Ca2+ influx, which in turn causes an increase in albumin permeability across the cultured monolayer of porcine pulmonary artery endothelial (PPAE) cells exposed to xanthine/xanthine oxidase (X/XO). Albumin permeability as well as the concentration of intracellular Ca2+ ([Ca2+]i) was increased by X/XO. A H2O2 scavenger (catalase), an iron chelator (o-phenanthroline), and a hydroxyl radical scavenger (dimethyl sulfoxide) inhibited these changes provoked by X/XO, in which intracellular iron-catalyzed hydroxyl radical generation was suggested to be involved. The increase in albumin permeability and [Ca2+]i continued once the PPAE cells were exposed to X/XO. The [Ca2+]i was decreased by a Ca2+ channel blocker, Ni2+, while the removal of Ni2+ increased [Ca2+]i again, suggesting the sustained Ca2+ influx through cell-membrane Ca2+ channels was responsible for the [Ca2+]i elevation. Ni2+ failed to inhibit albumin permeability sustained after the removal of X/XO. In contrast, SH-reducing agents (dithiothreitol and glutathione) inhibited the sustained permeability as well as Ca2+ influx. We concluded that the redox alteration of SH-groups in cell-membrane Ca2+ channels was involved in the increase in albumin permeability after exposure of the endothelial cells to oxidative stress.     

SKF96365和氯化镍对环匹阿尼酸诱导的大鼠PASMC［Ca2＋］i升高的影响

目的：研究SKF96365和氯化镍（NiCl2）对环匹阿尼酸（CPA）诱导的大鼠远端肺动脉平滑肌细胞（PASMC）内游离钙离子浓度（[Ca2＋]i）变化的影响。方法培养大鼠PASMC,运用荧光显微镜和InCyte细胞内钙浓度检测系统观测CPA、SKF96365和NiCl2对PASMC[Ca2＋]i的影响。结果含5μmol／L硝苯地平的无钙Krebs溶液孵育PASMC,10μmol／LCPA使PASMC[Ca2＋]i短暂小幅升高,恢复细胞外Ca2＋至2．5mmol／L后,10μmol／LCPA使PASMC[Ca2＋]i迅速显著升高;50μmol／LSKF96365和500μmol／LNiCl2均能明显抑制10μmol／LCPA引起的PASMC[Ca2＋]i升高,但对高钾（60mmol／LKCl）溶液引起的PASMC[Ca2＋]i升高无影响。结论CPA可致大鼠PASMC[Ca2＋]i升高,且能被SKF96365和NiCl2阻断,提示CPA可能诱发细胞外Ca2＋经钙池操纵性钙通道（SOCC）内流,SKF96365和NiCl2能选择性抑制SOCC活性使经SOCC的Ca2＋内流减少。     

Relationship of Intracellular Free Ca^2＋ Concentration and Calcium-activated Chloride Channels of Pulmonary Artery Smooth Muscle Cells in Rats under Hypoxic Conditions

Hypoxic pul monary hypertension ( HPH) ischaracterized by an elevation of pul monary vascularreaction and pul monary vascular reconstruction.Cytoplasmic free Ca2 concentration ([ Ca2 ]i)plays a key role in the regulation of vascular tone ,pul monary vasoconstriction and proliferation ofsmooth muscle cells[1]. The present study evalua-ted the role of [Ca2 ]iin the regulation of pul mo-nary vascular tone through regulation of calcium-activated chloride (Clca) channels in rats under a-cute h…     

Effects of nerve growth factor on intracellular free Ca2+ in oxygen/glucose-deprived cultures from cerebral cortex of fetal rats

ＩｔｉｓｗｅｌｋｎｏｗｎｔｈａｔｔｈｅｉｎｔｒａｃｅｌｕｌａｒＣａ２＋ｐｌａｙｓａｎｉｍｐｏｒｔａｎｔｒｏｌｅｉｎｂｏｔｈｐｈｙｓｉｏｌｏｇｉｃａｌａｎｄｔｏｘｉｃｏｌｏｇｉｃａｌｐｒｏｃｅｓｓｅｓ．ＡｄｉｓｒｕｐｔｉｏｎｏｆＣａ２＋ｈｏｍｅｏｓｔａ...     

灯盏花素对氯化钾诱导的培养平滑肌细胞内游离钙的影响

目的:观察灯盏花素对氯化钾诱导的培养的家兔平滑肌细胞内游离钙的影响.方法:用RF_5000荧光光度仪检测Fura-2AM负载的培养平滑肌细胞内游离钙的浓度.结果:在有细胞外钙存在时,静息条件下细胞内游离钙为330±100nmol·L-1,灯盏花素能轻度降低静息状态下细胞内游离钙,但无统计学意义.氯化钾50mmol·L-1将细胞内游离钙增加到420±104nmol·L-1,而灯盏花素10-6、10-5、3×10-5mol·L-1能进一步加强由氯化钾引起的细胞内游离钙的增加.然而,在无细胞外钙时,灯盏花素对细胞内游离钙却没有影响.结论:灯盏花素通过电压依赖性钙通道加强钙离子内流.     

内皮素对肺动脉平滑肌细胞第二信使系统的影响

在培养的猪肺动脉平滑肌细胞上观察内皮素-1 对第二信使系统的影响。实验发现内皮素-1促进肺动脉平滑肌细胞内钙释放,继之以外钙内流;使三磷酸肌醇骤增后下降;对细胞内环腺苷酸和环鸟苷酸量无明显影响。     

PDGF在低氧内皮细胞条件培养基促猪肺动脉平滑肌细胞增殖中的作用

为了探索低氧条件下猪肺动脉内皮细胞 (PAEC)对猪肺动脉平滑肌细胞 (PASMC)增殖调控关系 ,应用MTT比色法 ,DNA荧光测定法观察了外源性 PDGF促 PASMC增殖作用 ,以及 Suram in抑制 PDGF促增殖作用的效应 ,并在此基础上研究了猪肺动脉内皮细胞条件培养基 (HECCM)中 PDGF促 PASMC增殖的作用。结果表明 ,HECCM促 PASMC增殖作用的效应可被 Suramin部分抑制 ,由此证明 ,PDGF是低氧刺激 PAEC产生的一种促 PASMC增殖的重要因子。     

慢性缺氧对大鼠肺动脉平滑肌细胞形态的影响

目的 探讨慢性缺氧引起缺氧性肺血管收缩反应降低的机制。方法 采用计算机成像及图像分析系统，观察慢性缺氧培养的大鼠肺内动脉平滑肌细胞形态学变化的情况。结果 慢性缺氧后肺动脉平滑肌细胞中缺氧敏感型细胞的比例明显减小，中间混合型细胞比例明显增高，而缺氧不敏感型细胞的比例不变(P＜0．05)。结论 慢性缺氧可直接使肺动脉平滑肌细胞表型转化，由缺氧敏感细胞向中间混合型细胞转化。这可能是慢性缺氧时缺氧性肺血管收缩反应降低的机制之一。