肝细胞癌Autotaxin cDNA部分序列测定及其意义

进行肝癌Ａｕｔｏｔａｘｉｎ（ＡＴＸ）ｃＤＮＡ部分序测测定,观察ＡＴＸ ｍＰＲＮＡ在肝癌组织中的表达程序,以探讨ＡＴＸ在肝癌转移机理中的作用。方法 提取肝癌７７２１细胞株,５例正常肝组织,３２肝癌组织ＲＮＡ,参照黑色素瘤ＡＴＸ ｍＲＮＡ表达程序分析,同时７７２１细胞株ＲＴ－ＰＣＲ产物进行ｃＤＮＡ序列测定显示与黑色素具有９９。７％的同样性;癌组织ＡＴＸ ｍＲＮＡ表达显著高于正常肝组织。结论 肝癌及正常     

γ-谷氨酰转移酶mRNA亚型对肝细胞癌变的监测

目的 探讨γ-谷氨酰转移酶（GGT）mRNA亚型的转化与原发性肝癌（HCC）发生的关系,寻找肝癌早期诊断的新方法。方法 以逆转录聚合酶链反应方法检测正常对照组、非癌肝病组、肝癌组及肝转移癌肝组织及外周血的3种GGTmRNA亚型(A、B、C亚型)。结果 正常肝纤维、非癌肝病的肝组织及肝转移癌癌周组织主要的GGTmRNA类型为A亚型,肝癌组织、癌旁组织及远癌组织GGTmRNA-B亚型的阳性率显著高于正常肝脏及非癌肝癌的肝组织（P＜0．05）,癌组织GGT mRNA-A亚型阳性率明显低于正常对照及非癌肝病组（P＜0．05）。在26例HCC中有12例外周血中检出GGTmRNA-B亚型,甲胎蛋白阴性的10例HCC中有5例检出GGTmRNA-B亚型。结论 GGT mRNA亚型转化与肝癌发生有密切关系,分析GGT基因可望成为监测肝细胞癌变的灵敏方法。     

Expression of liver cancer associated gene HCCA3

AIM:To study and clone a novel liver cancer related gene,and to explore the molecular basis of liver cancer genesis.METHODS:Using mRNA differential display polymerssechain reaction(DDPCR),we investigated the difference ofmRNA in human hepstocallular carcinoma(HCC)and pairedsurrounding liver tissues,and got a gene probe.Byscreening a human placenta cDNA library and genornichomologous extend,we obtained a full-length cDNA namedHCCA3.We analyzed the expression of this novel gene in 42pairs of HCC and the surrounding liver tissues,anddistribution in human normal tissues by mssns of Northernblot assay.RESULTS:A full-length cDNA of liver cancer associated geneHCCA3 has been submitted to the GeneBank nucleotidesequence databases(Accession No.AF276707).Thepositive expression rate of this gene was 78.6%(33/42)inHCC tissues,and the clinical pathological data showed thatthe HCCA3 was closely associated with the invasion oftumor capsule(P=0.023)and adjacant small metastasissatellite nodules lesions(P=0.041).The HCCA3 was widelydistributed in the human normal tissues,which wasIntensively expressed In lungs,brain and colon tissues,while lowly expressed In the liver tissues.CONCLUSION:A novel full-length cDNA was cloned anddifferentiated,which was highly expressed in liver cancertissues.The high expression was closely related to thetumor invssivensss and metastasis,that may be the lateheredited change in HCC genssis.     

Abnormal β-catenin gene expression with invasiveness of primary hepatocellular carcinoma in China

AIM To study the abnormal expression of β-catenin gene and its relationship with invasiveness of primary hepatocellular carcinoma among Chinese people. METHODS Thirty-four hepatocellular carcinoma (HCC) specimens and adjacent para-cancerous tissues, 4 normal liver tissues were immunohistochemically stained to study subcellular distribution of β-catenin. Semiquantitive analysis of expression of β-catenin gene exon 3 mRNA was examined by RT-PCR and in situ hybridization. The relationship between expressions of both β-catenin protein, mRNA and clinicopathological characteristics of HCC was also analyzed. RESULTS Immunohistochemistry showed that all normal liver tissues and para-cancerous tissues examined displayed membranous type staining for β-catenin protein,occasionally with weak expression in the cytoplasm.While 21 cases (61.8%) of HCC examined showed accumulated type in cytoplasms or nuclei. The accumuled type Labling Index (LI) of cancer tissue and paracancarous tissue was (59.9 ± 26.3) and (18.3 ± 9.7)respectively (P＜0.01). Higher accumulated type LI was closely related with invasiveness of HCC. Results of RTPCR showed the β-catenin gene exon 3 mRNA Expression Index (El) of 34 HCCs was higher than that of paracancerous tissue and normal liver tissue. Using in situ hybridization, the signal corresponding to β-catenin gene exon 3 mRNA was particularly strong in cytoplasm of HCC when compared with those of para-cancerous and normal liver tissues. Over expression of β-catenin exon 3 was also found to be correlated with high metastatic potential of HCC. CONCLUSION Abnormal expression of β-catenin gene may contribute importantly to the invasiveness of HCC among Chinese people.     

肝癌组织和血清中钙囊素的表达及其意义

目的 研究人肝癌组织及血清中钙囊素(S100A11)的表达及其临床意义.方法 免疫组织化学法检测46例肝癌及其癌旁组织中S100A11表达,比较肝癌与癌旁组织中S100A11的阳性率,分析肝癌组织中S100A11表达水平与各临床参数之间的关系;同时检测肝癌(62例)、肝硬化(32例)、慢性肝炎(30例)患者及健康体检者(28名)血清中S100A11浓度,比较S100A11浓度与甲胎蛋白(AFP)、γ-谷氨酰转肽酶同工酶Ⅱ(GGT-Ⅱ)诊断肝癌的灵敏性及特异性.结果 S100A11在肝癌组织中的表达阳性率(78.3%)显著高于癌旁组织(19.6%,P<0.01),其表达水平与分化程度相关,分化程度越低表达水平越高.根据ROC曲线,当确定诊断界值为7.3μg/L时,S100A11在肝癌患者血清中的阳性率为30.6%,明显高于肝硬化、慢性肝炎及健康人(P值均<0.05).肝癌患者血清中S100A11与AFP、GGT-Ⅱ均无相关性,联合检测三项指标对肝癌诊断有互补性,可使诊断敏感性提高至84.5%.结论 S100A11可能与肝癌的发生发展有关.联合检测S100A11与AFP和GGT-Ⅱ可提高对肝癌的诊断敏感度.

Abstract：

Objective To explore the expression of calcium binding protein (S100A11) and its clinical significances in human hepatocellular carcinoma (HCC) tissue and blood plasma. Methods The expressions of S100A11 in 46 cases of HCC tissues and their paracancerous tissues were detected by immunohistochemistry. The relationship between S100A11 expression level in HCC tissues and clinical parameters was analyzed. The S100A11 expression levels in blood plasma of HCC patients (62 cases), liver cirrhosis patients (32 cases), chronic hepatitis patients (30 cases) and healthy subjects (30 cases) were detected. The sensitivity and specificity of S100A11, alpha fetoprotein (AFP) and γ-glutamyl transpeptidase Ⅱ (GGT-Ⅱ ) in HCC diagnosis were compared. Results The positive rate of S100A11 in HCC tissue (78. 3%) was significantly higher than that in paracancerous tissues (19. 6%) (P<0. 05). The expression level was correlated with the degree of differentiation, the lower differentiation degree with the higher expression level. According to ROC curve, if the cutoff points for diagnosis was set at 7. 3 μg/L, the positive rate of S100A11 in HCC patients blood plasma was 30. 6% , which was significantly higher than that in the blood plasma of patients with liver cirrhosis, patients with chronic hepatitis and healthy persons (P<0. 05). There was no correlation between S100A11 and AFP or GGT-Ⅱ in the blood plasma of HCC patients. These three indicators were complementary in HCC diagnosis, and the diagnostic sensitivity increased to 84.5% with combined detection. Conclusions S100All may be related to HCC genesis and development. The HCC diagnostic sensitivity may be increased with combined detection of S100All ,AFP and GOT- Ⅱ.     

肝癌组织甲胎蛋白表达特点及其基因片段的扩增分析

目的探讨肝癌组织甲胎蛋白(AFP)表达的病理学特点及其基因分析的临床价值。方法以免疫组织化学法研究AFP在肝癌及癌周组织中的胞内分布及其表达;并从肝癌的癌灶、癌旁组织中制备总RNA,以巢式聚合酶链反应(nested_PCR)扩增不同癌组织AFP_mRNA片段,以探讨AFP表达的临床病理学特征及其AFP_mRNA分析的应用价值。结果肝癌细胞及癌旁肝细胞的胞浆中,均可见AFP阳性的棕黄色颗粒,癌组织中AFP阳性表达率为73.3%,癌旁组织中为40.0%,中、低分化组肝癌中AFP的表达阳性率明显高于高分化组肝癌(P<0.05),且癌旁组织中AFP的表达强度明显低于肝癌的癌灶组织。肝癌组织、癌周组织的总RNA表达水平存在差别,癌组织中AFP_mRNA片段扩增全数阳性(100%),显著高于癌周组织(P<0.01)。结论肝癌不同组织中AFP表达存在差异,AFP_mRNA分析有助于肝癌的早期发现和诊断。     

Expression of alpha fetoprotein messenger RNA in BEL-7404 human hepatoma cells and effect of L-4 oxalysine on the expression

ＥｘｐｒｅｓｓｉｏｎｏｆａｌｐｈａｆｅｔｏｐｒｏｔｅｉｎｍｅｓｓｅｎｇｅｒＲＮＡｉｎＢＥＬ７４０４ｈｕｍａｎｈｅｐａｔｏｍａｃｅｌｓａｎｄｅｆｅｃｔｏｆＬ４ｏｘａｌｙｓｉｎｅｏｎｔｈｅｅｘｐｒｅｓｓｉｏｎＷＡＮＧＸｉｎｇＷａｎｇａｎｄＸＵ...     

Abnormal beta-catenin gene expression with invasiveness of primary hepatocellular carcinoma in China

AIM: To study the abnormal expression of beta-catenin gene and its relationship ith invasiveness of primary hepatocellular carcinoma among Chinese people. METHODS: Thirty-four hepatocellular carcinoma (HCC) specimens and adjacent para-cancerous tissues, 4 normal liver tissues were immunohistochemically stained to study subcellular distribution of beta-catenin. Semiquantitive analysis of expression of beta-catenin gene exon 3 mRNA was examined by RT-PCR and in situ hybridization. The relationship between expressions of both beta-catenin protein, mRNA and clinicopathological characteristics of HCC was also analyzed. RESULTS: Immuno-histochemistry showed that all normal liver tissues and para-cancerous tissues examined displayed membranous type staining for beta-catenin protein, occasionally with weak expression in the cytoplasm. While 21 cases (61.8%) of HCC examined showed accumulated type in cytoplasms or nuclei. The accumulated type Labling Index (LI) of cancer tissue and para-cancerous tissue was (59.9 +/- 26.3) and (18.3 +/- 9.7) respectively (P<0.01). Higher accumulated type LI was closely related with invasiveness of HCC. Results of RT-PCR showed the beta-catenin gene exon 3 mRNA Expression Index (EI) of 34 HCCs was higher than that of para-cancerous tissue and normal liver tissue. Using in situ hybridization, the signal corresponding to beta-catenin gene exon 3 mRNA was particularly strong in cytoplasm of HCC when compared with those of para-cancerous and normal liver tissues. Over expression of beta-catenin exon 3 was also found to be correlated with high metastatic potential of HCC. CONCLUSION: Abnormal expression of beta-catenin gene may contribute importantly to the invasiveness of HCC among Chinese people.     

黑色素瘤抗原-A1基因多态性及其在肝癌组织中的表达

目的了解黑色素瘤抗原(MAGE)-A1基因在肝癌组织中的表达状况,探讨该基因是否存在单核苷酸多态性(SNP).方法用RT-PCR方法检测49例原发性肝细胞性肝癌(HCC)患者肝癌组织MAGE-A1基因mRNA的表达情况,同时提取其中43例HCC患者的肝癌组织、癌旁组织及外周血细胞基因组DNA,PCR扩增MAGE-A1 DNA,并对上述PCR产物进行测序.结果49例患者中MAGE-A1 mRNA阳性22例,阳性率44.9%.43例HCC患者癌组织、癌旁组织和外周血细胞中扩增的KAGE-A1 DNA序列测定显示MAGE-A1基因存在两类变异:TAG有16例,变异发生率为37.2%,包括3个位置的变异:C159T,A272G,G393A,GTG变异有7例,发生率为16.3%,亦包括3个位点的变异即:A272G,C991T,A1125G.因为C991T没有改变编码的氨基酸,A1125G不在编码区,故仅A272G引起一个氨基酸的替换(T32A).结论MAGE-A1在HCC中高表达,表达率为44.9%.MAGE-A1 mRNA的表达与AFP无关.MAGE-A1基因存在三种SNP,但不影响其mRNA的表达.     

Mutation and overexpression of the beta-catenin gene may play an important role in primary hepatocellular carcinoma among Chinese people

AIM: To study the role of beta-catenin gene mutation and expression in hepatocellular carcinogenesis. METHOD: Thirty-four hepatocellular carcinoma (HCC) specimens and adjacent para-cancerous tissues, and four normal liver tissues were analyzed. Subcellular distribution of beta-catenin was examined by immunohistochemistry staining. Mutation and semiquantitative expression of beta-catenin gene exon 3 mRNA were detected by RT-PCR-SSCP and in situ hybridization. RESULT: Immunohistochemistry showed that all normal liver tissues and para-cancerous tissues examined showed membranous-type staining for beta-catenin protein, frequently with weak expression in the cytoplasm, but no beta-catenin accumulation in nuclei was found; while in liver cancer, 21 cases (61.8%) of HCC examined showed accumulated type in cytoplasms or nuclei. On SSCP, 15 cases (44.1%) of HCC altogether displayed three kinds of characteristic mutational mobility shifts. No abnormal shifting bands were found in tissues from normal liver or para-cancerous area. The beta-catenin gene exon 3 mRNA expression index of 34 HCCs was higher than that of para-cancerous tissue and normal liver tissue. Using in situ hybridization, the signal corresponding to beta-catenin gene exon 3 mRNA was particularly strong in cytoplasm of HCC when compared with those of paracancerous tissues and normal liver tissues. CONCLUSION: beta-catenin gene mutation and overexpression may have a critical role in malignant progression of hepatic carcinogenesis among Chinese people.     

Combined serum hepatoma-specific alpha-fetoprotein and circulating alpha-fetoprotein-mRNA in diagnosis of hepatocellular carcinoma

Introduction Hepatocellular carcinoma (HCC) is a major health problem throughout the world.[1-3] It ranks the fifth in frequency worldwideamong all malignancies and causes one million deaths annually,[4, 5] yet its incidence is increasing steadily in various countries.[6-8] The carcinogenesis of HCC is a multi-factorial, multi-step and complex process. Perhaps hepatitis B virus (HBV) infection is merely a carcinogenic factor, and is not related to the growth, infiltration and metastasis of…     

Ephrin-A1 expression contributes to the malignant characteristics of {alpha}-fetoprotein producing hepatocellular carcinoma

BACKGROUND AND AIMS: alpha-Fetoprotein (AFP), a tumour marker for hepatocellular carcinoma (HCC), is associated with poor prognosis. Using cDNA microarray analysis, we previously found that ephrin-A1, an angiogenic factor, is the most differentially overexpressed gene in AFP producing hepatoma cell lines. In the present study, we investigated the significance of ephrin-A1 expression in HCC. METHODS: We examined ephrin-A1 expression and its effect on cell proliferation and gene expression in five AFP producing hepatoma cell lines, three AFP negative hepatoma cell lines, and 20 human HCC specimens. RESULTS: Ephrin-A1 expression levels were lowest in normal liver tissue, elevated in cirrhotic tissue, and further elevated in HCC specimens. Ephrin-A1 expression was strongly correlated with AFP expression (r = 0.866). We showed that ephrin-A1 induced expression of AFP. This finding implicates ephrin-A1 in the mechanism of AFP induction in HCC. Ephrin-A1 promoted the proliferation of ephrin-A1 underexpressing HLE cells, and an ephrin-A1 antisense oligonucleotide inhibited the proliferation of ephrin-A1 overexpressing Huh7 cells. Thus ephrin-A1 affects hepatoma cell growth. cDNA microarray analysis showed that ephrin-A1 induced expression of genes related to the cell cycle (p21), angiogenesis (angiopoietin 1 and thrombospondin 1), and cell-cell interactions (Rho, integrin, and matrix metalloproteinases) in cultured hepatoma cells. These ephrin-A1 induced genes are also activated in HCC tissues that overexpress AFP. CONCLUSION: These findings suggest that the poor prognosis of patients with AFP producing HCC is partially caused by ephrin-A1 expression, which induces expression of genes related to tumour cell growth, angiogenesis, invasion, and metastasis.     

KLF6在肝细胞癌中的表达及在肝癌细胞系中对增殖和凋亡的影响

目的 检测抑癌基因KLF6在人肝细胞癌中的表达情况,探讨KLF6在肝癌细胞系中对细胞增殖及凋亡的影响.方法 分别用荧光定量PCR、RT-PCR 和Western blot 、免疫组织化学方法检测肝细胞癌、癌旁组织及正常肝组织中KL F6 基因mRNA 及蛋白水平的表达情况.构建KLF-6基因的真核表达质粒,用MTT法检...     

AXIN2基因在肝细胞癌中的表达及甲基化研究

目的 观察肝细胞癌(HCC)中AXIN2 mRNA表达水平,分析AXIN2基因甲基化水平对mRNA表达的影响及在HCC发生、发展中的意义.方法 收集手术切除的53例HCC和配对癌旁组织标本、7例正常肝组织标本和5种人肝癌细胞系.应用荧光定量PCR方法检测AXIN2mRNA水平的表达和AXIN2基因启动子区甲基化状态.结果 AXIN2在癌组织中的mRNA表达水平(0.1629±0.0679)低于癌旁组织(0.4155±0.2330),差异有统计学意义(Z=-2.567,P=0.010).HCC和癌旁组织中AXIN2基因甲基化水平(39.77%±3.89%和36.92%±2.81%)均高于正常肝组织(7.38%±2.40%,t 值分别=-3.663和-4.591,P值分别=0.009和0.007).5种人肝癌细胞系中AXIN2基因均呈高甲基化状态.AXIN2 mRNA表达水平与甲基化程度呈负相关(r=-0.458,P=0.032).TNMⅢ期HCC患者的AXIN2甲基化水平高于TNM Ⅰ和Ⅱ期患者(P=0.008).结论 AXIN2 mRNA表达水平下降与其高甲基化状态相关,AXIN2 mRNA低表达和启动子区异常甲基化可能是HCC发生、发展的重要机制之一.

Abstract：

Objective To investigate AXIN2 mRNA expression level in hepatocellular carcinoma (HCC) , and to analyze the effect of AXIN2 gene methylation status on its mRNA expression and HCC genesis and development. Methods Fifty-three surgical excised HCC specimens and paired adjacent non-cancerous specimens, seven normal liver specimens and five HCC cell lines were collected. The expression of AXIN2 at mRNA level and the methylation status of AXIN2 gene promoter were determined by quantitative PCR. Results The expression of AXIN2 mRNA was lower in HCC tissues (0.1629 + 0.0679) than that in adjacent non-cancerous tissues (0. 4155 + 0. 2330), and there was significant difference (Z= -2. 567, P = 0. 010). The methylation level of AXIN2 gene in HCC and adjacent non-cancerous tissues (39. 77% ±3. 89%, and 36. 92% ±2. 81%) was significantly higher than that in normal liver tissues (7. 38% ±2. 40% , t=-3. 663 ,P = 0. 009;t= -4. 591 ,P = 0. 007).AXIN2 gene was hypermethylated in all five HCC cell lines. There was a negative correlation between AXIN2 mRNA expression level and the degree of methylation ( r = -0. 458, P = 0. 032). The methylation level was higher in TNM Ⅲ patients of HCC than that in TNM Ⅰ and Ⅱ patients (P =0.008). Conclusion The down-regulation of AXIN2 gene mRNA expression is correlated with its hypermethylation status. The low expression of AXIN2 mRNA and the abnormal methylation of promoter may be one of the important mechanism of HCC genesis and development.     

Recurrence or metastasis of HCC:predictors, early detection and experimental antiangiogenic therapy

AIM To investigate the predictors for recurrence or metastasis of HCC, and to evaluate the effect of antiangiogenic therapy on the growth of transplantable human HCC in nude mice. METHODS RT-PCR was used to measure the expression of matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) in 56 pairs of nontumorous liver and tumor samples. Sixty blood samples from human HCC were examined by nested RT-PCR to find out AFP mRNA. Recombinant human endostatin and polyclonal antibody against VEGF were administered to treat human HCC transplanted in nude mice. RESULTS Thirty of 56 HCC samples showed stronger expression of MMP-9 in tumorous tissues than in nontumorous tissues. Fifteen of the 26 patients with relative expression level of MMP-9 more than 0.34 developed tumor recurrence or metastasis, whereas only 7 of 30 patients with relative expression level less than 0.34 developed tumor recurrence (P＜0.05).There was no significant difference in the relative expression level of VEGF between patients with postoperative recurrence or metastasis and those without recurrence. AFP mRNA was detectable in 53.3% of patients with HCC. The sensitivity and specificity of AFP mRNA as a marker to detect hematogenous dissemination of HCC cells was 81.8% and 84.4%, respectively. Recombinant human endostatin and polyclonal antibody against VEGF inhibited the growth of transplantable HCC in nude mice by 52.2% and 45.7%, respectively.CONCLUSION MMP-9 expression in HCC correlates with the postoperative recurrence or metastasis of HCC. Patients with high level of MMP-9 expression in HCC are susceptible to metastasis. AFP mRNA could serve as an indicator of hematogenous spreading of HCC cells in circulation and a predictor of recurrence or metastasis of HCC. Antiangiogenesis may be an adjuvant therapy for HCC.     

hTERT基因表达在肝细胞肝癌发生发展中的作用及意义

目的　研究端粒酶催化亚单位 (h TERT)基因表达在肝细胞肝癌 (HCC)发生发展中的作用及意义。方法　应用免疫组化 (SP法 )和半定量逆转录聚合酶链反应 (RT- PCR)同步检测 6 2例 HCC及其对应癌旁组织、8例正常肝组织中 h TERT及 h TERTm RNA的表达情况 ,分析 h TERT基因表达与 HCC临床病理参数的关系。结果　 h TERT蛋白主要定位于肝癌细胞胞浆内 ,偶见核内染色。h TERT和 h TERTm RNA在 HCC中的阳性率分别为 88.71%、82 .2 6 % ,分别明显高于癌旁肝组织的阳性率 9.6 8%、6 .4 5 % ,差异具有非常显著性 (P <0 .0 1)。 h TERT及其 m RNA在 8例正常肝组织均未见表达。统计学分析显示 ,HCC中的 h TERT基因表达与肿瘤分期分级、门静脉有无癌栓、血清中甲胎蛋白表达水平、是否合并 HBV感染等有关 (P <0 .0 5 )。结论　h TERT基因可能在 HCC的发生发展中起着重要作用 ,其表达有望成为 HCC的重要分子生物学指标。     

肝癌细胞表达差异片段MRG 98.2的临床意义

目的 筛选肝细胞癌（HCC）相关基因,探讨其在HCC发病中的临床意义。方法 用差异显示技术对比研究正常肝组织、HCC组织及癌旁肝组织之间mRNA的表达差异,以肝cDNA片段MRG98．2为探针,对10例HCC及癌旁肝组织进行斑点印渍分析,并通过逆转录聚合酶链反应（RT－PCR）方法检测这些标本血管内皮生长因子（VEGF）mRNA的表达水平。结果 从HCC组织中获得的差示片段MRG98．2在7例HCC标本中表达阳性（70％）,癌旁肝组织弱表达（2／10）或无表达。VEGF mRNA在7例MRG98．2 阳性表达的HCC组织中的6例表达上调。结论 MRG98．2是HCC相关的基因片段,其表达与VEGF mRNA相关,并可能与肿瘤浸润转移、患者的预后不良有关。     

Expression of gap junction genes connexin 32, connexin 43 and their proteins in hepatocellular carcinoma and normal liver tissues

AIM To investigate the significance and mechanism of cx32 mRNA, cx43 mRNA and their proteins in hepatocarcinogenesis.METHODS Sixty-one cases of HCC and 14 cases of normal liver tissues were detected by immunohistochemical and in situ hybridization (ISH) methods.RESULTS In HCC grades Ⅰ,Ⅱ,Ⅲ and normal liver tissues, the positive rates of Cx32 protein were 55.6%, 42.1%, 18.2% and 92.9%,respectively. The detection rates of Cx43 protein were 44.4%, 26.3%, 12.1% and 78.6%,respectively. There was significant difference in Cx32 and Cx43 protein between HCC and normal liver tissues (P＜0.01). ISH the positive rates of cx32 mRNA shown by ISH in HCC grades Ⅰ,Ⅱ,Ⅲ and normal liver tissues were 88.9%, 84.2%,87.9% and 92.9%, respectively. Those of cx43 mRNA were 77.8%, 78.6%, 78.8% and 85.7%,respectively. There was no statistical difference in the positive rates of cx32 mRNA and cx43 mRNA between HCC and normal liver tissue (P＞0.05).CONCLUSION The aberrant location of Cx32 and Cx43 proteins could be responsible for progression of hepatocarcinogenesis, and the defect of cx genes in post-translational processing might be the possible mechanism.     

应用基因芯片研究肝细胞癌组织差异基因表达谱

目的：应用基因芯片技术研究原发性肝细胞癌组织中的差异基因表达谱改变，以寻找肝细胞癌相关基因。方法：抽提正常肝组织和肝癌组织中的mRNA来制备探针，经杂交、洗涤后，通过计算机扫描分析正常肝组织和肝癌组织基因表达谱的差异情况。结果：在10000个候选基因中，筛选出102条差异表达基因，表达上调的有42条，表达下调的有60条。未知基因12条。结论：基于DNA微阵矩技术的肿瘤基因表达谱分析能够高通量筛选与肝癌发生发展相关的基因。