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1.
OBJECTIVE
To assess the antioxidant capacity of aqueous extracts of buds and flowers of Lonicera japonica Thunb. (BLJ and FLJ) using in vitro assays.METHODS
We assessed the in vitro antioxidant activities of aqueous extracts of BLJ and FLJ and compared with that of classical antioxidants vitamin C and butylated hydroxytoluene, using several well-established methods including the 1,1-diphenyl-2-picryl-hydrazyl assay, 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) assay, reducing power assay, fluorescence recovery after photo-bleaching assay, β-carotene bleaching assay, ferric thiocyanate assay, and thiobarbituric acid method.RESULTS
The aqueous extracts of both BLJ and FLJ had similarly potent antioxidant capacity. There were no significance differences between BLJ and FLJ in all the assays.CONCLUSION
The aqueous extracts of both BLJ and FLJ have antioxidant activity with comparable efficacy. These findings suggest that both BLJ and FLJ may have the potential as natural antioxidants. 相似文献2.
Kafeel Ahmad Amjad Ali Waqar Ahmed Afridi Ramla Somayya Muhammad Jawad Ullah 《中医杂志(英文版)》2018,38(3):433-438
OBJECTIVE
To investigate the antimicrobial activity, hemagglutination and phytotoxic activity of crude ethanolic and aqueous extracts of Seriphidium kurramense.METHODS
The extracts were analyzed by agar well diffusion assays against five bacterial species: Staphylococcus aureus (S. aureus), methicillin-resistant S. aureus, Escherichia coli, Klebsiella pneumoniae, Bacillus subtilis, and Salmonella typhi. The extracts were also screened against six fungal species — Aspergillus niger, Aspergillus flavus, Alternaria solani, Rhizoctonia solani, Fusarium solani and Pleurotus florida — using the agar tube diffusion method. Additionally, hemagglutination and phytotoxic activities of the crude ethanolic and aqueous extracts were assessed.RESULTS
The crude ethanolic and aqueous extracts showed dose-dependent inhibition of the various tested fungal and bacterial strains. No hemagglutination activity was observed. Both the ethanolic and aqueous extracts showed dose-dependent phytotoxic activity toward Lemna minor.CONCLUSION
The crude ethanolic and aqueous extracts of Seriphidium kurramense possess good antimicrobial and phytotoxic activities, but no hemagglutination activity. 相似文献3.
Zhi Na YIN Wen Jia WU Chong Zhen SUN Hui Fan LIU Wen Bo CHEN Qi Ping ZHAN Zhuo Gui LEI Xuan XIN Juan Juan MA Kun YAO Tian MIN Meng Meng ZHANG Hui WU 《Biomedical and environmental sciences : BES》2019,32(1):11-21
Objective
A strain of Aspergillus niger (A. niger), capable of releasing bound phenolic acids from wheat bran, was isolated. This strain was identified by gene sequence identification. The antioxidant and anti-inflammatory capacity of ferulic acid released from wheat bran by this A. niger strain (FA-WB) were evaluated.Methods
Molecular identification techniques based on PCR analysis of specific genomic sequences were conducted; antioxidant ability was examined using oxygen radical absorbance capacity (ORAC), cellular antioxidant activity (CAA) assays, and erythrocyte hemolysis assays. RAW264.7 cells were used as a model to detect anti-inflammatory activity.Results
The filamentous fungal isolate was identified to be A. niger. ORAC and CAA assay showed that FA-WB had better antioxidant activity than that of the ferulic acid standard. The erythrocyte hemolysis assay results suggested that FA-WB could attenuate AAPH-induced oxidative stress through inhibition of reactive oxy gen species (ROS) generation. FA-WB could significantly restore the AAPH-induced increase in intracellular antioxidant enzyme activities to normal levels as well as inhibit the intracellular malondialdehyde formation. TNF-α, IL-6, and NO levels indicated that FA-WB can inhibit the inflammation induced by lipopolysaccharide (LPS).Conclusion
Ferulic acid released from wheat bran by a new strain of A. niger had good anti-inflammatory activity and better antioxidant ability than standard ferulic acid. 相似文献4.
Chen Cen Wang Fengqin Xiao Wen Xia Zhining Hu Guang Wan Jianbo Yang Fengqing 《中医杂志(英文版)》2017,37(1):64-75
OBJECTIVE
To evaluate the anti-platelet aggregation effects of extracts from 31 Traditional Chinese Medicines (TCM) with the property of activating blood and resolving stasis in terms of TCM theory.METHODS
The 31 TCMs extracts were prepared using water, 90% ethanol and ethyl acetate., and the effects on anti-platelet aggregation were tested on a platelet aggregation analyzer in vitro with adenosine 5'-diphosphate, bovine thrombin and arachidonic acid (AA) as aggregation inducers, respectively. Aspirin was the positive control.RESULTS
Lots of the tested TCMs had inhibitory effects with concentration-dependent manner on platelet aggregations induced by various agonists. Especially, some of the TCMs such as Chuanxiong (Rhizoma Chuanxiong), Yanhusuo (Rhizoma Corydalis Yanhusuo) and Danshen (Radix Salviae Miltiorrhizae) showed good anti-platelet aggregation effect similar or higher than that in positive control group.CONCLUSION
The study provided scientific references that several TCMs such as Chuanxiong (Rhizoma Chuanxiong), Yanhusuo (Rhizoma Corydalis Yanhusuo) and Danshen (Radix Salviae Miltiorrhizae), possess the property of anti-platelet aggregation. 相似文献5.
Guo Yuhong Di Tingting Zhao Jingxia Xu Xiaolong Li Ping Huang Lijuan Liu Qingquan 《中医杂志(英文版)》2018,38(4):579-584
OBJECTIVE
To observe the effects of Fuxin decoction in rats with heart failure induced by doxorubicin injections.METHODS
We employed a doxorubicin-induced heart failure model to investigate the effect of Fuxin decoction. Rats were orally administered with Fuxin decoction from 10 to 40 mg/kg. Organization structure changes were assessed by histology, circulating levels of oxidative stress-related biomarkers and inflammatory cytokines were detected via enzyme-linked immunosorbent assay.RESULTS
Administration of Fuxin decoction improved the growth retardation in doxorubicin hydrochloride injection rats. Cardiac involvement was alleviated by Fuxin decoction, as well as changes of congestion systemic circulation in this rat models. Furthermore, Fuxin decoction regulates immune responses and suppresses oxidation reaction in doxorubicin hydrochloride-induced rats.CONCLUSION
Fuxin decoction might be an interesting candidate for congestive heart failure. 相似文献6.
Guesmi Fatma Saidi Issam Soussi Rawya Hfaiedh Najla Landoulsi Ahmed 《Biomedical and environmental sciences : BES》2019,32(1):22-33
Objective This research aimed to evaluate the protective effects of bioactive compounds such as phenolic acids, flavonoids, and tannins present in four species extracted with methanol. Methods The total phenolic content of the methanolic extracts was measured spectrophotometrically. The effect of the extracts on cell viability in U266 cells was measured. The effects of extracts on free radical scavenging were assessed by the DPPH test and FRAP assay. Antibacterial effects of the natural products in this report were investigated by using the disc diffusion method. Results Our results clearly demonstrated that the methanolic extracts were characterized by a high amount of phenolic compounds. It has been speculated that ME-TA and ME-TAl exhibit a significant(P < 0.05) and dose-dependent antiradical potential. The exposure of cells to high doses of extracts almost completely suppressed cell growth in vitro. ME-TA and ME-TAl showed significant cytotoxic effects at a concentration of 100 μg/mL in the U266 cell line. ME-TAl and ME-CF inhibited the growth of B. subtilis and S. aureus, respectively, to the same extent as 10 μg/μL of chloramphenicol at a concentration of 1 mg/mL. Conclusion Overall, these results suggest that plants used in traditional medicine have a novel application as free radical scavengers, bacterial inhibitors and tumor suppressors. 相似文献
7.
Akuodor Godwin Christian Ezeonu Chinonyelum Thecla Essien Augustine Dick Asika Ebere Chile Chilaka Kingsley Chimsorom Nwachukwu Daniel Ckukwu Nwobodo Ndubuisi Nwobodo 《中医杂志(英文版)》2017,37(4):431-435
Objective
To evaluate the in vivo antiplasmodial activity and the oral acute toxicity of the Bombax buonopozense root bark aqueous extract.Methods
The in vivo antiplasmodial activity of the root bark aqueous extract of Bombax buonopozense against early and established rodent malaria infections in chloroquine sensitive Plasmodium berghei strain in mice was investigated, and oral acute toxicity of the aqueous root bark extract of Bombax buonopozense was also evaluated in mice.Results
The findings of this study revealed significant (P < 0.05) and dose dependent decrease in parasitaemia in the parasitized groups treated with varying doses of the extract (50–200 mg/kg p.o.) in both suppressive and curative tests. There was also significant decrease in parasitaemia density in the chloroquine treated group. The aqueous extract was found no toxicity in mice and the oral LD50 was determined to be greater than 5000 mg/kg.Conclusion
Bombax buonopozense root bark aqueous extract possesses potent antiplasmodial activity and may therefore, serve as potential sources of new antimalarial agents. 相似文献8.
OBJECTIVE
To assess the medication adherence reporting in clinical trials the field of Traditional Chinese Medicine (TCM) and the impact factors of medication adherence.METHODS
Reviewed and evaluated were all randomized clinical trials in the field of TCM in treatment of type 2 diabetes mellitus published in Chinese journals in 2012, in terms of their medication adherence, adherence measurement, and impacted factors of adherence.RESULTS
Finally 124 studies were included. None studies reported the medication adherence. The factors impacting medication adherence couldn't be analyzed due to none reporting adherence.CONCLUSION
Medication adherence reporting was poor in clinical trials in TCM research. Establishing standards for adherence assessment and reporting may be one of the important steps to improve the quality of clinical studies. 相似文献9.
OBJECTIVE
To investigate the effect and safety of Gandouling plus low-dose D-penicillamine for treating patients with Wilson's disease (WD) who have neurological symptoms.METHODS
WD patients with neurological symptoms were divided into two groups: a treatment group (n = 53) and a control group (n = 50). The treatment group received anti-copper therapy with a combination of Gandouling and low-dose D-penicillamine (10 mg/kg), whereas the control group was with conventional dose D-penicillamine (20 mg/kg) monotherapy. The clinical efficacies, adverse reactions, and results of the various hematological and biochemical investigations were recorded and analyzed statistically.RESULTS
Overall, 98.11% of the WD patients treated with the combined therapy experienced alleviation of their neurological condition (paralleled by a significantly improved Global Assessment Scale score or remained stable). Their white blood cell and platelet counts stabilized, and their liver function was improved or remained stable. The combined therapy also obviously promoted improved 24-h urinary copper excretion. Only 15.09% of the WD patients with the combined therapy experienced adverse reactions, including neurological deterioration in one case (1.89%) and hepatic worsening in one case (1.89%), which was less frequent than that in the control group given conventional-dose D-penicillamine monotherapy.CONCLUSION
Treating WD patients with neurological symptoms using Gandouling plus low-dose D-penicillamine is effective and safe. 相似文献10.
Wang Chunli Zhang Liang Wu Meimei Jing Yuntiao Lai Xiaoping Huang Song Zhang Xiaojun 《中医杂志(英文版)》2018,38(4):523-534
OBJECTIVE
To examine the antioxidative and hepatoprotective activities of the effective fraction separated from the fruit of Livistona chinensis (FLC) and to explore the possible mechanism.METHODS
The antioxidative activities of the various fractions separated from FLC were analyzed by in vitro DPPH (1,1-diphenyl-2-picrylhydrazyl radical 2, 2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl, DPPH) radical and superoxide anion free radical (O2 ?) scavenging assays. The potential hepatoprotective effects of the ethyl acetate fraction separated from FLC (EFLC) were examined in LO2 cells and mice. LO2 cells were incubated with EFLC and exposed to H2O2-induced oxidative stress. BABL/C mice were orally administered EFLC for consecutive 7 d, and fulminant hepatitis was induced by cauda vein injection of ConA on day 7.RESULTS
EFLC showed prominent antioxidative effects in DPPH - and O2 - scavenging assays. EFLC pretreatment effectively protected LO2 cells from H2O2-induced oxidative stress by decreasing apoptosis and raising the levels of antioxidant enzymes. Additionally, EFLC protected mice against ConA-induced fulminant hepatitis by markedly reducing the serum levels of alanine transaminase and aspartate aminotransferase, attenuating histological damage of the mouse liver, and elevating the levels of glutathione peroxidase and total superoxide dismutase, while decreasing the contents of methane dicarboxylic aldehyde, tumor necrosis factor-α, and interleukin-1β in the mouse liver. Furthermore, EFLC pretreatment downregulated the protein expression of B-cell lymphoma 2 (Bcl-2) associated X protein, caspase-3, caspase-8, Fas, and FasL, while upregulating the protein expression of Bcl-2 in the mouse liver.CONCLUSION
These findings revealed that EFLC effectively protected against in vivo and in vitro liver injury, and the mechanism is closely associated with its antioxidative and anti-apoptotic properties. 相似文献11.
12.
Zhang Lihong Zhuang Zhihong Sun Yanan Ma Shuhua Yang Weifeng Lei Hongtao Zuo Junling Ouyang Jingfeng Wang Yi 《中医杂志(英文版)》2017,37(3):308-313
Objective
To investigate the neural differentiation capacity of water extraction of velvet antler.Methods
Velvet antler (Cervus Nippon Temminck) polypeptide (VAP) was used to differentiate neural stem cells (NSCs) towards neurons in the study. Firstly, we obtain the polypeptides of VAP by water extraction. Secondly, we observed the morphology, assayed the factors in the media by enzyme-linked immunosorbent assay, and detected the special neural molecules by immunofluorescence staining. NSCs were cultured on the cell climbing film. After neuronal differentiation, differentiated NSCs were mounted for immunocytochemistry with immunofluorescence technique.Results
The differentiating cells look like neuron, some special factors, such as Glial cell line-derived neurotrophic factor, nerve growth factor, in the media can be detected while differentiated neuron -like cells can express the special neural molecules.Conclusion
Differentiation of NSCs towards neurons can be induced by velvet antler polypeptide. 相似文献13.
Sami Ullah 《中医杂志(英文版)》2017,37(4):444-451
Objective
To evaluate the anticancer, antioxidant and antimicrobial activities along with total phenolic and flavonoids contents extracted from Lespedeza bicolor indigenous to Khyber Pukhtoonkhwa, Pakistan.Methods
Anticancer activity against human lung carcinoma (LU-1) and human prostate carcinoma (LnCap), antimicrobial against Aspergillus fumigates, Aspergillus niger, Fusarium solani, Mucor sp Klebsiella pneumonia and Enterococcus and antioxidant activity on 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) and Hydrogen peroxide radicals scavenging activity were assessed in methanol extract of Lespedeza bicolor.Results
Lespedeza bicolor aerial parts and root extract showed IC50 value of 12.5 and 50 μg/mL against LU-1 whereas, ≤ 12.5 and 12 μg/mL were calculated against LnCap cell line. Minimum inhibition concentration (MIC) value of 20–35 μg/mL has been observed against Aspergillus fumigates, Aspergillus niger, Fusarium solani and Mucor sp in comparision with 1–2.5 μg/mL of Terbinafine used as a standard fungicide. MIC value of 20 and 35 μg/mL of Lespedeza bicolor aerial parts and root extract against bacterial pathogen Klebsiella pneumonia and 20–50 μg/mL against Enterococcus has been measured. DPPH radical scavenging activity of Lespedeza bicolor with IC50 values of ≤ 50 μg/mL and ≤ 200 μg/mL was observed whereas, hydrogen peroxide scavenging activity with IC50 values of ≤ 25 μg/mL for aerial parts and ≤ 50 μg/mL for the root extract of Lespedeza bicolor has been shown.Conclusion
These data suggested that the methanolic extract of Lespedeza bicolor could be potential candidates for natural antioxidants and anticancer. 相似文献14.
OBJECTIVE
To evaluate the application of Traditional Chinese Medicine (TCM) Four-diagnostic Auxiliary Apparatus in disease diagnosis.METHODS
The liver cancer patients and healthy controls were recruited from Shanghai Integrated Chinese and Western Medicine Hospital and Beijing University of Traditional Chinese Medicine, respectively. Then, the included subjects were diagnosed by the Four-diagnostic auxiliary apparatus.RESULTS
Thirty liver cancer patients and 30 paired healthy controls were enrolled in this study. Based on the apparatus, the pulse wave velocity was significantly higher in patients compared with controls (P < 0.05). The number of patients with purple tongue and ecchymosis were more than controls (P < 0.05). The number of patients (10%) with yellow tongue coating were higher than the controls (0%). Patients were inclined to be with water type and fire type constitution.CONCLUSION
TCM Four-diagnostic auxiliary apparatus can be applied in clinical diagnosis of body constitution and health status of subjects. It promotes the accuracy and speed for disease diagnosis and TCM standardization. 相似文献15.
Wei Guining Chu Shifeng Su Qibiao Su Hua Lin Meiyu He Fei Lu Wenjie Lu Guoshou Huang Zhoufeng Tan Xiao Lin Xiao Zeng Xianbiao Wei Baowei Chen Naihong 《中医杂志(英文版)》2018,38(1):12-21
OBJECTIVE
To investigate the antidepressant-like effect of active fraction of Polyrhachis vicina Roger (AFPR) in a rat depression model, and to elucidate the underlying mechanism.METHODS
AFPR was extracted with ethanol followed by petroleum ether. Its antidepressant-like effect was investigated in mice by tail suspension test (TST), forced swimming test (FST) and open field test (OPT). A repeated dose of reserpine (0.5 mg/kg, daily for 14 d) was used to establish a rat depression model. Fluoxetine was used as positive control agent. The effect of AFPR on reserpine-induced ptosis, hypothermia and akinesia, the levels of monoamines and their metabolites, and the activity of monoamine oxidase (MAO) in hippocampus and prefrontal cortex were determined.RESULTS
Administration of AFPR by gavage at 160 and 320 mg/kg significantly reduced the duration of immobility in the FST and TST, and did not affect locomotor activity in the OPT. In the reserpine-induced depression model, AFPR attenuated anhedonia, demonstrated by reversing hypothermia, akinesia and sucrose consumption. AFPR significantly increased the concentration of monoamines, including dopamine, serotonin, noradrenaline and acetylcholine.CONCLUSION
AFPR normalized the metabolism rates of noradrenaline, serotonin and dopamine, and the activity of MAO, which were altered by chronic reserpine exposure. The findings suggest that modulation of the monoaminergic neurotransmitter system likely underlies the antidepressant-like effect of AFPR. 相似文献16.
Suni Lee Shoko Yamamoto Yasumitsu Nishimura Hidenori Matsuzaki Kei Yoshitome Tamayo Hatayama Miho Ikeda Min YU Nagisa Sada Naoko Kumagai-Takei Takemi Otsuki 《Biomedical and environmental sciences : BES》2018,31(5):335-342
Objective The changes in serum adipokines and cytokines related to oxidative stress were examined during 3 months ‘Off to On' and ‘On to Off' periods using negatively charged particle-dominant indoor air conditions(NCPDIAC).Methods Seven volunteers participated in the study,which included ‘OFF to 3 months ON' periods(ON trials) for a total of 16 times,and ‘ON to 3 months OFF'(OFF trials) periods for a total of 13 times.Results With the exception of one case,serum amyloid A(SAA) levels decreased significantly during the ON trials.Conclusion Considering that SAA is an acute phase reactive protein such as C reactive protein(CRP),this observed decrease might indicate the prevention of cardiovascular and atherosclerotic changes,since an increase in high-sensitive CRP is associated with the subsequent detection of these events. 相似文献
17.
Objective
To use the theory of Traditional Chinese Medicine (TCM) to identify the major symptom patterns of Parkinson's disease.Methods
Journal databases were searched for relevant articles in the last 30 years. Articles were reviewed for symptom patterns of Parkinson's disease and analyzed using frequency analysis, cluster analysis, and other methods of data extraction.Results
The analyses indicated that the most frequent symptom patterns of Parkinson's disease are yin deficiency of kidney and liver, deficiency of Qi and blood, phlegm heat and wind stirring, blood stasis and wind stirring, and deficiency of yin and yang.Conclusion
Taken together, the analyses identified the primary symptom patterns of Parkinson's disease as Yin deficiency of kidney and liver, deficiency of Qi and blood, phlegm heat and wind stirring, and blood stasis and wind stirring. 相似文献18.
Hai Qin FANG Zhou YU Yuan ZHI Jin FANG Chen Xi LI Yi Mei WANG Shuang Qing PENG Xu Dong JIA 《Biomedical and environmental sciences : BES》2018,31(5):363-375
Objective
The present study was undertaken to evaluate the subchronic toxicity of lanthanum and to determine the no observed adverse effect level (NOAEL), which is a critical factor in the establishment of an acceptable dietary intake (ADI).Methods
In accordance with the Organization for Economic Co-operation and Development (OECD) testing guidelines, lanthanum nitrate was administered once daily by gavage to Sprague-Dawley (SD) rats at dose levels of 0, 1.5, 6.0, 24.0, and 144.0 mg/kg body weight (BW) per day for 90 days, followed by a recovery period of 4 weeks in the 144.0 mg/kg BW per day and normal control groups. Outcome parameters were mortality, clinical symptoms, body and organ weights, serum chemistry, and food consumption, as well as ophthalmic, urinary, hematologic, and histopathologic indicators. The benchmark dose (BMD) approach was applied to estimate a point of departure for the hazard risk assessment of lanthanum.Results
Significant decreases were found in the 144.0 mg/kg BW group in the growth index, including body weight, organ weights, and food consumption. This study suggests that the NOAEL of lanthanum nitrate is 24.0 mg/kg BW per day. Importantly, the 95% lower confidence value of the benchmark dose (BMDL) was estimated as 9.4 mg/kg BW per day in females and 19.3 mg/kg BW per day in males.Conclusion
The present subchronic oral exposure toxicity study may provide scientific data for the risk assessment of lanthanum and other rare earth elements (REEs). 相似文献19.
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