艾灸肝俞、期门穴治疗慢性乙型肝炎的临床研究

目的为探讨艾灸肝俞、期门穴治疗慢性乙型肝炎患者的安全性及疗效。方法将100例慢性乙型肝炎患者随机分为治疗组（n=50）及对照组（n=50）。治疗组给予基础保肝加用艾灸肝俞、期门穴治疗,对照组给予基础保肝治疗。全部病例连续治疗12周。结果治疗组患者治疗后食欲不振、恶心、呕吐、腹胀、乏力、肝肿大等临床症状与对照组比较明显好转（P〈0．05）;治疗组ALT（32．15±8．01）U／L,对照组（62．32±20．50）U／L,两组比较差异有统计学有意义（P〈0．05）;治疗组AST（35．28±8．82）U／L,对照组（65．36±21．20）U／L,两组比较差异有统计学有意义（P〈0．05）;TBiL复常率、HBeAg阴转率、HBV—DNA阴转率与对照组比较,差异均有显著性（P〈0．05）。结论艾灸肝俞、期门穴治疗慢性乙型肝炎患者疗效好,且安全、无创、简单易行,无副作用,为中西医结合治疗肝病开创一条简单可行之路。     

Pathologic analysis on hyperplasia of mammary gland with different syndromes based on infrared radiation temperature of acupoints

OBJECTIVE:To explore the pathologic characteristics of hyperplasia of the mammary gland(HMG) by observing differences in infrared radiation temperature of points of HMG in patients with different syndromes compared with healthy controls.METHODS:AFLIRSystems Therma CAM P30 infrared thermal camera was used to detect the infrared temperature of Shanzhong(CV 17),Qimen(LR 14),Zhongwan(CV 12),Qihai(CV 6),Guanyuan(CV 4),Taixi(KI 3),and Taichong(LR 3) in 113 patients with HMG.Of these patients,71 were placed in the Liver Qi stagnation group,34 were placed in the Dysfunction of conception and thoroughfare vessels group,and 8 were placed in the Phlegm and blood stasis in combination group.The infrared radiation temperature of each point in the patients was compared with that of healthy controls,and the differences in the infrared radiation temperatures of the points in the patients were analyzed.RESULTS:Overall,the bilateral corresponding point in both the controls and patients exhibited no significant difference in infrared radiation temperature.In all cases,the infrared radiation temperature of the points from proximal to distal tended to decrease.In a comparison of the patients and controls,the infrared radiation temperature of the trunk points Shanzhong(CV 17),Qimen(LR 14),Zhongwan(CV12),Qihai(CV6),and Guanyuan(CV 4) of the patients was higher than that of the controls,while the infrared radiation temperature of the lower extremity points Taixi(KI 3) and Taichong(LR 3) was lower than that of the controls.Of these points,Shanzhong(CV 17)(P=0.0368),Zhongwan(CV 12)(P=0.0028),Qihai(CV 6)(P=0.0085),and Guanyuan(CV4)(P=0.0018) showed significant differences.In a comparison of the corresponding point on the same side in the Liver Qi stagnation group and controls,the infrared radiation temperature of Shanzhong(CV17)(P=0.0089),right-side Qimen(LR 14)(P=0.0382),Zhongwan(CV 12)(P= 0.0000),Qihai(CV 6)(P=0.0011),and Guanyuan(CV 4)(P=0.0000) of the patients was significantly higher than that of the controls,while the differences in the infrared radiation temperature of the other points were not statistically significant(P= 0.0833-0.8397).In a comparison of the corresponding point on the same side in the Dysfunction of conception and thoroughfare vessels group and controls,the infrared radiation temperature of left-side Taichong(LR 3)(P=0.0048),right-side Taichong(LR 3)(P=0.0329),left-side Taixi(KI 3)(P= 0.0171),and right-side Taixi(KI 3)(t=0.544,P= 0.0165) of the patients was significantly lower than that of the controls,while the differences in the infrared radiation temperature of the other points were not statistically significant(P=0.3793-0.9197).In a comparison of the corresponding point on the same side in the Phlegm and blood stasis in combination group and controls,the infrared radiation temperature of Shanzhong(CV 17),Qimen(LR 14),Qihai(CV 6),Guanyuan(CV 4),Taixi(KI 3),Taichong(LR 3),and Zhongwan(CV 12) tended to increase,but without statistical significance(P=0.175-.759).CONCLUSION:The corresponding points of HMG patients with different syndromes are in different deficiency/excess states.Changes in the infrared radiation temperature of the trunk points Shanzhong(CV 17),Qimen(LR 14),Zhongwan(CV 12),Qihai(CV 6),and Guanyuan(CV 4) are closely related to the pathological characteristics of the Liver Qi stagnation syndrome of HMG patients,while changes in the infrared radiation temperature of the lower extremity points Taixi(KI 3) and Taichong(LR 3) are closely related to the pathological characteristics of the Dysfunction of conception and thoroughfare vessels syndrome of HMG patients.On the whole,HMG patients with Liver Qi stagnation syndrome are characterized by "upper excess," and those with Dysfunction of conception and thoroughfare vessels syndrome are characterized by"lowerdeficiency."     

Effect of stimulating the acupoints Feishu (BL 13) and Dazhui (GV 14) on transdermal uptake of sinapine thiocyanate in asthma gel

Objective

To investigate the effect of stimulating the acupoints Feishu (BL 13) and Dazhui (GV 14) on the transdermal uptake of sinapine thiocyanate contained in a gel used for the management of asthma.

Effect of scar-producing moxibustion at the acupoints Zusanli (ST 36) and Feishu (BL 13) on neutrophil-to-lymphocyte ratio and quality of life in patients with non-small-cell lung cancer: A randomized,controlled trial

OBJECTIVE

To evaluate the effect of heat stimulation via scar-producing moxibustion at the acupoints Zusanli (ST 36) and Feishu (BL 13) on the neutrophil-to-lymphocyte ratio (NLR) and quality of life in patients with non-small-cell lung cancer (NSCLC).

Effect of warming moxibustion Tianshu (ST 25, bilateral) and Qihai (CV 6) for the treatment of diarrhea-dominant irritable bowel syndrome: a patient-blinded pilot trial with orthogonal design

Objective

To investigate the effects of warming moxibustion Tianshu (ST 25, bilateral) and Qihai (CV 6) in patients with diarrhea-dominant irritable bowel syndrome (D-IBS) by assessing predominant factors, and determining the best factor combinations and their interactions. To identify the optimal quantity of warming moxibustion Tianshu (ST 25, bilateral) and Qihai (CV 6) to achieve optimal efficacy.

Differences in thermal effects of moxibustion at Zusanli(ST 36) and Hegu(LI 4) on various facial areas in healthy people

OBJECTIVE:To study the effects of temperature on different facial areas by suspended moxibustion at two points,Zusanli(ST 36) and Hegu(LI 4),and probe the phenomena underlying self-regulation in the human body after thermal stimulation.METHODS:Thirty healthy volunteers accepted moxibustion over Zusanli(ST 36) and Hegu(LI 4),and the order of moxibustion points was randomly determined.Moxibustion method:suspension of moxibustion over Zusanli(ST 36) and Hegu(LI 4) on both sides was performed using an ignited moxa stick stuck in a support for 20 min.Observation method:An infrared thermal image of the face was taken before and after suspended moxibustion using a CK350 medical infrared thermal imaging instrument.Data analysis:A thermal microscopic section view system(TMTSys) was used to analyze the change in temperature in special facial areas.Statistical analysis was carried out using SPSS 14.0 software.RESULTS:Before moxibustion was suspended,the facial thermal image showed a T-shaped thermal area related to the vascular distribution with even temperature and good symmetry on both sides.Suspended moxibustion over Zusanli(ST 36) have a very significant increase in temperature at the forehead,around the nose,at the corners of the mouth,and at the cheeks and lips(P<0.01).Suspended moxibustion over Hegu(LI 4) also have a significant(P<0.05) increase in temperature around the nose,the corners of the mouth,the cheeks,and lips,where has a new high temperature area was formed(P<0.01).Suspended moxibustion over Hegu(LI 4) raised the temperature at the middle point of the lips more obviously than did Zusanli(ST 36) in the same person,(P<0.05).After 10 min of moxibustion over Zusanli(ST 36) and Hegu(LI 4),the change in temperature in the facial area reached its peak value.CONCLUSIONS:Facial infrared thermography of healthy people revealed a T-shaped thermal area reflecting a physiological thermal area.Moxibustion over Zusanli(ST 36) or Hegu(LI 4) raised the temperature in this facial T-shaped thermal area.Hegu(LI 4) led to the formation of a new thermal area in the lips.The time required for moxibustion to regulate human body temperature was 10 min.     

Effect of catgut implantation at acupoints on GABAB and mGluR1 expressions in brain stem of rats with spasticity after stroke

ObjectiveTo investigate the effect of catgut implantation at acupoints on the expressions of γ-amino butyric acid B receptor (GABA_B) and metabotropic glutamate receptor 1 (mGluR1) in the brain stem of rats with spasticity after stroke.MethodsIn total, 60 male Sprague-Dawley rats were randomly divided into three groups: a sham group (n=10), a model group (n=25) and a treatment group (n=25). The rats in both the model group and the treatment group were subjected to middle cerebral artery occlusion to establish a model of focal cerebral ischemia. Rats with limb-spasm met the inclusion criteria. Only the left carotid artery was isolated in sham group rats. Three days after modeling, the treatment group was subjected to catgut implantation at Dazhui (GV 14), Guanyuan (CV 4), and Zhongwan (CV 12). Neurological deficit symptoms were assessed with the Zea-Longa neurological deficit score. The Modified Ashworth Scale (MAS), and isolated muscle tone were used to evaluate spasticity before and after treatment. Immunohistochemistry was applied to determine the expression of GABA_B and mGluR1 in the rat brain stem after treatment.ResultsAfter treatment, neural impairment symptoms had significantly improved in the treatment group when compared to the model group (P<0.05). Both MAS and isolated muscle tone in the treatment group were significantly decreased when compared with the model group (P<0.05), and were also lower than before treatment. GABA_B expression was significantly higher and mGluR1 was lower in the treatment group when compared with the model group (P<0.01 and P<0.05, respectively).ConclusionCatgut implantation at Dazhui (GV 14), Guanyuan (CV 4), and Zhongwan (CV 12), can relieve limb spasticity by increasing the expression of GABA_B and reducing the expression of mGluR1 in the brain stem of rats after stroke.     

Effects of electroacupuncture at Guanyuan (CV 4) or Sanyinjiao (SP 6) on hypothalamus-pituitary-ovary axis and spatial learning and memory in female SAMP8 mice

OBJECTIVE

To investigate the effects of electroacupuncture (EA) at the Guanyuan (CV 4) or Sanyinjiao (SP 6) acupoints on the hypothalamus-pituitary-ovary (HPO) axis and spatial learning and memory in female mice.

Effect of electroacpuncture of Neiguan (PC 6) and Tianquan (PC 2) on skin temperature,blood perfusion,and adrenoceptor response in rats with acute myocardial ischemia

Objective

To investigate the effect of electroacupuncture (EA) of acupoints Neiguan (PC 6) and Tianquan (PC 2) on the skin temperature, blood perfusion, and alpha1- and beta2-adrenoreceptor (α₁-AR and β₂-AR) protein and mRNA level in rats with acute myocardial ischemia.

辅助性T细胞17(Th17)的特征及其在自身免疫性疾病中的作用

 辅助性T细胞分为调节性T细胞、Th1、Th2和Th17亚型。Th17细胞是新近发现的一个细胞亚群,其分泌的IL-17A和IL-17F是重要的致炎因子。本文综述了Th17分化和维持所需的微环境、Th17与其他主要免疫细胞的相互作用及Th17对自身免疫性疾病的影响,重点阐述了Th17相关的IL-23与Th1相关的IL-12之间的关系、树突状细胞对Th17分化的影响及Th17细胞对各种常见自身免疫性疾病的影响,通过对Th17细胞的深入研究将有助于认识自身免疫性疾病及进一步寻找新的治疗靶点。     

针灸至阴穴矫正异常胎位的应用探讨

至阴是对胎位不正有重要影响的特异性穴位,历代文献均有针灸至阴转胎的记载,一般孕妇施用3～4次即可使胎位转正,转胎成功率可达90%以上。针灸刺激方法众多,其中常用的艾条灸法、艾炷灸法、温针灸法、穴位贴敷法、激光照射法效果显著,目前以艾条灸法为主。现介绍针灸几种常用刺激方法的具体施术操作,探讨矫正胎位的机制,明确临证应用中需要注意的问题,使这种简便实用、安全有效的转胎方法得以普及推广。     

开颅和腹部手术患者苏醒期脑过度灌注现象的比较

 目的 使用颈静脉球部血氧饱和度和经 颅多普勒监测,研究手术苏醒期患者脑血流动力学的变化。方法 随机选择择期在全麻下行开颅脑肿瘤切除术和腹部手术的患者,分为开颅手术组（Ⅰ组）（n＝50）和腹部手术组（Ⅱ组）（n＝50）。Ⅰ组患者术前行颈静脉球部置管,分别测术前,术后拔管即刻,拔管后30,60,90和120min时大脑中动脉平均血流速度（mean velocity of middle cerebral artery, MCA Vm）,平均动脉压（mean arterial pressure, MAP）及颈静脉球部血氧饱和度（blood oxygen saturation of jugular vein bulb,SjvO2 ）。Ⅱ组分别测量术前,术后拔管即刻,拔管后30,60,90和120min时的MCA Vm和MAP。 结果 Ⅰ组患者MCA Vm从术后拔管即刻到拔管后2h间均较术前明显升高（P<0.05）,Ⅱ组患者术后MCA Vm无明显升高,两组MCA Vm在各时点差异有统计学意义（P<0.05）。Ⅰ组患者术后SjvO2较术前显著升高,并维持至拔管后2h（P<0.05）。Ⅰ组MCA Vm和同时点MAP值均不存在线性相关,和同时点SjvO2值在术后各点均存在正相关关系（P<0.05）。结论 仅Ⅰ组术后发生脑过度灌注,脑过度灌注程度与血压无线性相关关系。     

针刺丰隆单穴及其配穴调节高脂血症大鼠血脂的效应比较

目的比较针刺丰隆单穴和其配穴调节高脂血症大鼠血脂的效应差异。方法采用高脂饮食饲料喂养制备高脂血症大鼠模型。将40只SD大鼠随机分为空白组、模型组、丰隆单穴组和丰隆配穴组(丰隆、关元、内关),每组10只。空白组每天给予基础饲料喂养,其余三组每天给予高脂饲料喂养。喂养2周后断尾取血测血脂。之后,给予丰隆单穴组、丰隆配穴组针刺治疗5周。治疗结束后,所有大鼠腹腔静脉取血检测血脂。结果丰隆单穴组及配穴组治疗前后的总胆固醇(TC)降低差值均较模型组有显著性差异(P<0.05)。丰隆配穴组治疗前后的低密度脂蛋白(LDL)降低差值、及高密度脂蛋白(HDL)升高差值均较模型组和丰隆单穴组有显著性差异(P<0.05)。结论针刺丰隆单穴和其配穴(丰隆、关元、内关)均可明显降低高脂血症大鼠的TC水平。但针刺丰隆配穴在降低高脂血症大鼠的LDL水平、及升高HDL水平方面,较针刺丰隆单穴有明显优势,说明针刺丰隆、内关和关元三穴在调节血脂方面有很好的协同作用。     

电针刺激大肠俞及足三里穴对肝缺血再灌注大鼠肠道屏障功能的影响

目的 探究术前电针(electric acupuncture,EA)刺激大肠俞及足三里穴,是否对肝缺血再灌注(ischemia-reperfusion,I/R)大鼠的肠道屏障功能具有保护作用.方法 32只SD大鼠随机分为假手术组(Sham组)、缺血再灌注组(I/R组)、穴位刺激缺血再灌注组(AS+I/R组)、非穴位刺激...     

IL-18基因105A/C多态性与长江以南汉族人群系统红斑狼疮(SLE)的关联性

 目的  探讨IL-18(interleukin-18)基因105A/C多态性与中国长江以南汉族人群系统性红斑狼疮(systemic lupus erythematosus,SLE)易感性之间的关系。方法  采用病例对照研究设计,收集病例对照各193例,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对IL-18基因105位点进行基因分型,在不同的遗传模式下分析105位点基因多态性与SLE的相关性,用χ2检验分析105A/C多态性与SLE临床表型间的相关性。 结果  IL-18基因105位点A等位基因在两组人群中的分布差异有统计学意义(OR=1.815,95% CI=1.151-2.863),在相加和隐性模型下也发现105A/C多态性与SLE有关(相加:OR=1.828,95% CI=1.152-2.900;隐性:OR=1.855,95% CI=1.131-3.044),且调整年龄、性别等因素后,在两种模型下的相关性仍然存在(相加:OR=1.987,95% CI=1.181-3.343;隐性:OR=1.960,95% CI=1.111-3.456)。此外,本研究显示补体C3C4降低患者A等位基因的频率上升(OR=1.737,95% CI=1.001-3.014),但经Bonferroni校正后差异不具有统计学意义( Pcorr = 0.336)。 结论  在中国长江以南汉族人群中IL-18基因105A/C多态性可能与SLE易感性有关,且A等位基因对SLE可能有危险性效应。     

Effect of electroacupuncture at Ximen (PC 4) and Hegu (LI 4) on expression of Akt in rats with myocardial ischemia-reperfusion injury

Objective

To investigate the effect of electroacupuncture (EA) at acupoints on the pericardium meridian on the expression of phosphorylated Akt (p-Akt) protein in rat myocardium after ischemia and reperfusion.

电针“心俞-神门”对急性心肌缺血模型大鼠心肌与海马组织Glu、Asp和NR1表达的影响

目的观察电针“心俞-神门”配穴对急性心肌缺血(acute myocardial ischemia,AMI)模型大鼠心肌和海马组织谷氨酸(Glu)、天冬氨酸(Asp)和N-甲基-D-天冬氨酸(N-methyl-D-aspartate,NMDA)受体亚基NMDAR1(NR1)的影响,探讨俞原配穴的协同效应,及其对AMI致脑损伤保护的作用机制。方法将60只健康雄性SD大鼠随机分为假手术组、模型组、电针“神门”组(神门组)、电针“心俞”组(心俞组)和电针“心俞+神门”组(心神组),每组12只。除假手术组外,其他4组大鼠选用冠状动脉左前降支结扎法建立AMI模型。干预组每天电针治疗1次,20 min/次,共治疗1周,假手术组和模型组不作干预治疗。观察分析心电图ST段变化;ELISA法检测各组大鼠心肌和海马组织中Glu、Asp浓度;实时荧光定量PCR检测各组大鼠心肌和海马组织中NR1 mRNA表达。结果与假手术组比较,模型组大鼠心电图ST段明显抬高(P<0.01),心肌、海马Glu、Asp浓度和NR1 mRNA表达均显著上升(P<0.01);与模型组比较,3组电针组治疗后ST段明显降低(P<0.01),心神组心肌和海马Glu、Asp浓度、NR1 mRNA表达均明显下降(P<0.01),神门组、心俞组心肌和海马NR1 mRNA表达及心肌Asp、海马Glu浓度均显著下降(P<0.01);与神门组比较,心神组心肌Asp浓度和海马Glu、Asp浓度及心肌和海马的NR1 mRNA表达均明显下降(P<0.05或P<0.01);与心俞组比较,心神组心肌和海马Asp浓度、NR1 mRNA表达明显下降(P<0.05或P<0.01)。Pearson相关分析显示,电针治疗后心肌、海马组织中Glu浓度与NR1 mRNA表达呈正相关。结论电针俞原配穴“心俞-神门”能够改善AMI模型大鼠心肌缺血状态,降低心肌组织和海马组织的兴奋性氨基酸Glu、Asp和NR1水平,可能是其发挥保护心肌缺血致脑损伤的作用机制之一。     

健脾化痰方加温灸中脘治疗轻中度稳定期肺脾气虚型COPD患者的疗效及生存质量评价

目的:观察健脾化痰方加温灸中脘治疗肺脾气虚型稳定期轻中度慢性阻塞性肺疾病(COPD)患者的临床疗效及其对患者生存质量的影响。方法:90例轻中度稳定期COPD患者随机分为综合组(健脾化痰方+温灸中脘+西医基础治疗)、中药组(健脾化痰方+西医基础治疗)和对照组(西医基础治疗),每组30例。治疗8周后,观察三组的临床疗效及主要临床症状(包括咳嗽、咯痰、气短/呼吸困难、哮鸣音)改善情况,同时采用蔡氏改良呼吸疾病量表评价三组患者的生活质量。结果:①综合组的临床总有效率为86.7%,中药组为86.7%,对照组为70.0%,综合组和中药组的疗效均明显优于对照组(P<0.01,P<0.05)。②治疗后,三组患者的主要临床症状较治疗前均明显改善(P<0.05,P<0.01),且综合组对咳嗽、咯痰的治疗效果显著优于对照组(P<0.01),中药组对咯痰的疗效亦显著优于对照组(P<0.01)。③治疗后,综合组和中药组患者的生存质量量表总均分(TMS)较治疗前均显著降低(P<0.01),且综合组、中药组患者的TMS较对照组亦显著降低(P<0.01);综合组患者的F1S、F2S、F3S和F4S量表评分较治疗前均显著降低(P<0.01),中药组患者的F1S、F3S、F4S量表评分较治疗前亦显著降低(P<0.01);且综合组和中药组患者的F3S、F4S量表评分较对照组显著降低(P<0.05)。结论:健脾化痰方加温灸中脘穴能显著改善肺脾气虚型轻中度稳定期COPD患者的临床症状及生存质量。     

Notch信号通路基因JAG1、ADAM17和ADAM10与先天性心脏病(CHD)的相关性

 目的  探索Notch通路的配体JAG1和限速酶ADAM10和ADAM17与先天性心脏病(congenital heart disease,CHD)发病的相关性。方法  选取来自山东、上海两地的1 053例CHD患者血液样本和1 000例对照样本,对这3个基因的5′启动子区和3′-UTR区进行了全测序,并进行统计分析及简要功能验证。结果  检测到7个SNP (含2个新发SNP位点Ch2:9695908T/C和Ch20:10655928T/C)和1个单倍型组合具有显著性频率差异。双荧光素酶报告基因试验表明其中ADAM17启动子区的rs3811592M rs13415726M rs3811591M这个连锁单倍型可显著下调基因表达;而JAG1启动子区的rs7264849M则可显著上调基因表达。结论  这些SNP突变可能影响Notch信号的强弱,从而与心脏发育异常相关。     

大气细颗粒物对大鼠白细胞介素17表达的影响及连花清瘟的干预作用

目的  探讨大气细颗粒物（PM2.5）急性暴露对大鼠肺部损伤的作用,及高、中、低剂量连花清瘟对其的保护作用。方法  48只Wistar大鼠,随机分为4个实验组（PM2.5染尘组及高、中、低剂量连花清瘟干预组）和2个对照组（空白对照组及生理盐水对照组）。由石家庄市环境监测中心提供空气细颗粒物,制备PM2.5悬液,采用气管内滴注法。空白对照组无任何干预措施;生理盐水对照组给予气管滴注生理盐水（1 ml/kg）;染尘组给予一次性气管内滴入PM2.5悬液（7.5 mg/kg）;3组干预组首先分别连续灌胃给予低（生药2 g/kg）、中（生药4 g/kg）、高（生药8 g/kg）剂量的连花清瘟溶液4 d,第4天灌胃给药后分别经气管滴入PM2.5悬液（7.5 mg/kg)。应用酶联免疫分析（ELISA）法测定各实验组动物血清白细胞介素17（IL-17)及肺表面活性蛋白A（SP-A）水平,并在光镜下观察肺组织病理形态变化。结果  空白对照组和生理盐水对照组间血清IL-17及SP-A差异无统计学意义（P >0.05）,染尘组血清IL-17及SP-A水平较生理盐水对照组及空白对照组显著升高,差异有统计学意义（P <0.05）;高、中、低剂量干预组较染尘组血清IL-17及SP-A水平均有不同程度降低,中高剂量组差异有统计学意义（P <0.05）。结论  PM2.5急性暴露可以导致大鼠肺部损伤,连花清瘟对肺部损伤有保护作用。