共查询到20条相似文献,搜索用时 171 毫秒
1.
[目的]探讨羟基磷灰石(HA)中掺锶对成骨细胞增殖、分化和矿化的影响,以及HA材料中适宜的掺锶量。[方法]用掺锶量分别为1%,5%和10%的HA生物陶瓷粉末及纯HA生物陶瓷粉末制备的浸提液培养SD大鼠成骨细胞,在不同时间点检测成骨细胞增殖、碱性磷酸酶(ALP)活性、核心结合因子(cbfal)基因表达,以及矿化结节形成的情况。[结果]各组成骨细胞的增殖无明显差别(P0.05);细胞培养第14、21d,掺锶各组成骨细胞的ALP活性、cbfalmRNA表达,及矿化结节数量等方面均显著高于HA组(P0.01),其中以掺锶量5%组最高,但与10%组相比无显著差异。[结论]HA中掺锶能上调成骨细胞cbfalmRNA的表达,促进成骨细胞分泌碱性磷酸酶,从而促进成骨细胞的分化、矿化,促进骨形成。详细的作用机制及HA中最佳的掺锶量尚需进一步的研究。 相似文献
2.
目的 探讨辛伐他汀(simvastatin,SIM)联合雷奈酸锶(strontium ranelate,SR)治疗对大鼠成骨细胞及破骨细胞生物学特性的影响.方法 分离和培养大鼠成骨细胞和破骨细胞;使用SIM或SR和较低剂量SIM联合较低剂量SR处理大鼠成骨细胞和破骨细胞;用Western blot方法检测成骨细胞和破骨... 相似文献
3.
成人成骨细胞与多孔羟基磷灰石联合培养观察 总被引:1,自引:0,他引:1
目的:观察成人成骨细胞与多孔羟基磷灰石(hydroxypatite,HA)的相容性及细胞在HA上的生长情况。方法:来自成人骨髓的成骨细胞与多孔HA联合培养,倒置显微镜、扫描电镜观察细胞生长情况。结果:成人成骨细胞与多孔HA有良好的相容性,细胞在HA微孔表面生长良好。结论:以HA为载体,可望制成有生物活性的人工骨。 相似文献
4.
目的 体外观察钛金属材料不同表面微形态对兔成骨细胞生物学行为的影响.方法 采用激光扫描、喷砂、机械加工和抛光技术在钛金属基片上制备四种不同的表面微形态,激光扫描表面(LS)为50μm宽度的平行微沟槽,机械加工表面(MS)为100 μm宽度大环形沟槽,两种表面均为规则纹理,喷砂表面(SS)为完全粗糙表面,抛光表面(PS)为光滑表面;扫描电镜观察表面形态特征并测定表面形态的粗糙度(Ra),分别在不同基片表面接种兔成骨细胞,培养12 d,测定细胞24 h黏附情况、12 d增殖情况、11 d碱性磷酸酶活性,并通过扫描电镜和荧光显微镜观察细胞形态,统计分析各组间的差异.结果 细胞黏附LS=SS>MS=PS,细胞增殖SS>LS>MS=PS,ALP活性LS=SS>MS=PS,在LS和SS表面上的细胞黏附、增殖及ALP活性均高于MS和PS表面(P<0.05).结论 钛金属材料表面微形态对兔成骨细胞的黏附、增殖、分化以及细胞形态产生影响,细胞级别的规则微沟槽表面为成骨细胞提供了一个更适合的生长环境. 相似文献
5.
目的研究含锶羟基磷灰石对骨质疏松症大鼠的影响。方法制备羟基磷灰石(HA)和10%含锶羟基磷灰石(10% SrHA,即HA中Sr与Ca的摩尔比例为1:9)种植体,以扫描电镜(SEM)、能量色散X射线能谱仪(EDX)、X射线衍射(XRD)检 测材料表面形貌特征。实验中将20只雌性SD大鼠均行双侧卵巢切除术,12周后将所有动物随机平均分为两组,并分别在 大鼠右侧股骨远端植人羟基磷灰石(HA)种植体和10%含锶羟基磷灰石(10% SrHA)种植体。植人术后8周,收集标本行显 微CT( micro-CT)和组织学分析。结果实验所得数据采用SPSS17. 0软件进行统计学分析。在micro~CT检验的结果中,相比 于HA种植体组,10% SrHA种植体组的相对骨体积提高了 42. 6%,骨结合率提高了 47. 1%,平均骨小梁数量提高了 27. 3%, 平均骨小梁厚度提高了 31. 5%,平均骨小梁连接密度提高了 37. 4%,而平均骨小梁间距降低了 20. 1%。在组织学检验结果 中,10%SrHA组的相对骨面积密度提高了 47. 6%。结论以HA种植体作为对照,10% SrHA种植体可以提高骨密度和骨结 合率,同时改善种植体周的骨小梁微结构。 相似文献
6.
7.
观察雷奈酸锶这种新型的抗骨质疏松药,在模拟微重力环境对成骨细胞凋亡的影响.方法 细胞凋亡是利用4,6-二氨基-2-苯基吲哚所染细胞核形态的改变来辨认,利用旋转细胞培养系统模拟微重力环境.结果 模拟微重力环境可以促进成骨细胞的凋亡,雷奈酸锶可以纠正模拟微重力环境对成骨细胞凋亡的影响.结论 在模拟微重力环境雷奈酸锶对成骨细胞生存具有保护作用,这为雷奈酸锶治疗微重力环境骨丢失提供了理论和实验证据. 相似文献
8.
目的 观察新型的抗骨质疏松药雷奈酸锶在模拟微重力环境下对成骨细胞增殖功能的影响.方法 利用沿水平轴连续回转(30 r/min)细胞培养系统模拟微重力环境,使用MTT比色法或台盼兰染色、细胞计数法观察小鼠成骨样细胞MC3T3-E1的增殖情况.结果 模拟微重力环境可以降低MC3T3-E1增殖功能,雷奈酸锶在模拟微重力环境可以增强MC3T3-E1增殖功能.结论 在模拟微重力环境下雷奈酸锶对成骨细胞增殖功能具有保护作用,为雷奈酸锶治疗微重力环境骨量丢失提供了理论和实验证据. 相似文献
9.
纤维多孔钛微球复合纳米锶磷灰石修复骨缺损的实验研究 总被引:2,自引:0,他引:2
目的 探讨纤维多孔钛微球复合纳米锶磷灰石修复骨缺损的能力及其作用机制.方法 6月龄雄性SD大鼠24只,体重(545±22)g.在双侧股骨髁部使用慢速钻钻取直径2 mm贯通双侧皮质的冠状轴洞性骨缺损.以纤维多孔钛微球复合纳米锶磷灰石填充左侧骨缺损,以单纯纤维多孔钛微球填充右侧.术后1、2、4、8周分别处死6只大鼠行X线、组织学及骨组织形态计量学观察,并进行比较分析.结果 影像学结果表明,两侧骨缺损修复效果均良好.组织学显示,纤维多孔钛微球允许骨长入,且左侧微球内新生骨多于右侧.骨组织形态计量学观察显示,随时间延长,左侧新生骨量逐渐增多,2、4、8周新生骨量存在差异,而4、8周时左侧与右侧的新生骨量比较,差异有统计学意义.结论 纤维多孔钛微球具有良好的生物相容性及骨传导性,可作为骨缺损修复的支架材料;纳米锶磷灰石可增强纤维多孔钛微球修复骨缺损的能力. 相似文献
10.
Objective To evaluate the effect of titanium fiber mesh microballoons combined with nano-Sr-HAP on repair of bone defects in vivo. Methods The experimental model was set up by creating drill defects in the bilateral femoral condyle of 24 SD rats [6 months, with the mean weight of (545±22 g)]. The left femoral defects were filled with titanium fiber mesh microballoons combined with nano-Sr-HAP, and the right with pure metal scaffolds. At the 1st, 2nd, 4th, and 8th week after surgery, the specimens were killed and taken the X-ray, histology, and histomorphology observation. The results were contrasted and ana-lyzed. Results X-ray observation showed that the bone defects in both sides were repaired. Histological re-sults showed that the titanium fiber mesh microballoons allowed the new bones growing inside, and more new bones in the left implants compared with those in the right sides. The results of histomorphometrical analysis confirmed that neo-bones in the left sides gradually increased as time passed. There were statistic differences in neo-bones at the 2nd, 4th, and 8th week, and the differences between the two sides were statistically sig-nificant at the 4th and 8th week. Conclusion As titanium fiber mesh microballoons used in this experiment have good biocompatibility and osteoconduction ability, they can be used as scaffold for bone defect repair; and Nano-Sr-HAP can enhance the repair ability of titanium fiber mesh. 相似文献
11.
Verberckmoes SC Behets GJ Oste L Bervoets AR Lamberts LV Drakopoulos M Somogyi A Cool P Dorriné W De Broe ME D'Haese PC 《Calcified tissue international》2004,75(5):405-415
In a previous experimental study using a chronic renal failure rat model, a dose-related multiphasic effect of strontium (Sr) on bone formation was found that could be reproduced in an in vitro set-up using primary rat osteoblasts. The results from the latter study allowed us to distinguish between a reduced nodule formation in the presence of an intact mineralization at low Sr-doses (1 g/ml) and an interference of the element with the hydroxyapatite (HA) formation at high doses (20–100 g/ml). To further investigate the latter effect of Sr on physicochemical bone mineral properties, an in vitro study was set up in which the UMR-106 rat osteosarcoma cell line was exposed to Sr, added to the cell culture medium in a concentration range varying between 0–100 g/ml. Temporal growth and functionality of the culture was investigated by measurement of the alkaline phosphatase activity and calcium (Ca) concentration in the culture medium (used as an index of Ca-incorporation, i.e., HA formation) at various time points. At the end of the culture period (14 days post-confluence), samples of the mineralized cultures were taken for further analysis using X-ray diffraction (XRD) and Fourier Transform Infra-Red Spectroscopy (FTIR). Synthetic HA doped with various Sr concentrations (based on the cell culture and previous experimental studies and yielding Sr/(Sr+Ca) ratios ranging from 0–60%), was prepared and examined for crystal growth and solubility. Crystal size was assessed using scanning electron microscopy (SEM). Ca incorporation indicated a reduced mineralization in the 20 and 100 g/ml Sr groups vs. controls. Sr-doped synthetic HA showed a significant dose-dependent reduction in crystal growth, as assessed by SEM, and an increase in solubility, apparent from 12.7% Sr/(Sr+Ca) on. Moreover, in both mineralized cultures and synthetic HA, XRD and FTIR analysis showed a reduced crystallinity and altered crystal lattice at similar concentrations. These new data support our previous in vivo and in vitro findings and point to a potential physicochemical interference of Sr with HA formation and crystal properties in vivo. 相似文献
12.
13.
The purpose of this study was to use the concept of metastable equilibrium solubility (MES) to describe the anomalous solubility behavior of carbonated apatite (CAP) in the presence of solution strontium. A CAP sample (4.8 wt% CO3, synthesized at 70°C) was prepared by precipitation. Baseline MES distributions were determined in a series of 0.1 M acetate buffers containing only calcium and phosphate (no strontium) over a broad range of solution conditions. In order to assess the influence of strontium, MES profiles were then determined in a similar fashion with 20, 30, 40, 50, 60, 70, and 80% of the solution calcium being replaced on an equal molar basis by solution strontium. From the compositions of the equilibrating buffer solutions, ion activity products (IAPs) of the form Ca10-nSrn(PO4)6(OH)2 (n = 0–10) were calculated in an attempt to determine the correct function governing the dissolution of the CAP preparation. The results demonstrate the following important findings: (a) at high solution strontium/calcium ratios (i.e., when 60% or more of the solution calcium was replaced by strontium), the MES profiles in all the experiments were found to be essentially superimposable when the solution IAPs were calculated using the stoichiometry of Ca6Sr4(PO4)6(OH)2, and (b), at low solution strontium/calcium ratios (i.e., when 40% or less of the solution calcium was replaced by strontium), the stoichiometry yielding MES data superpositioning was found to be that of hydroxyapatite. When other stoichiometries were assumed, good superpositioning of the data was not possible. 相似文献
14.
Hwang JS Chen JF Yang TS Wu DJ Tsai KS Ho C Wu CH Su SL Wang CJ Tu ST 《Calcified tissue international》2008,83(5):308-314
The aim of this study was to assess the efficacy and safety of strontium ranelate in the treatment of postmenopausal women
with osteoporosis in Taiwan. In this 12-month multicenter, randomized, double-blind, placebo-controlled study, 125 women with
osteoporosis were randomly given either strontium ranelate 2 g daily or placebo. Lumbar spine, femoral neck, and total-hip
bone mineral density (BMD) and biochemical markers of bone turnover were measured; adverse events and tolerability were recorded
and assessed. Subjects treated with strontium ranelate showed significant increases in BMD of 5.9% at the lumbar spine, 2.6%
at the femoral neck, and 2.7% at the total hip, while the placebo group exhibited no significant change at 12 months. Serum
level of a formation marker (bone-specific alkaline phosphatase) was also significantly increased at 6 and 12 months. Thus,
although the sample size and the treatment duration of this study could not show its effect of reducing osteoprotic fractures,
strontium ranelate showed bone protection effects by increasing BMD and concentrations of a bone formation marker. Safety
assessment revealed adverse events were mild and not significantly different from placebo.
An erratum to this article can be found at 相似文献
15.
本研究利用QUANTIMET—970图象分析仪对26例胆管癌细胞核形态学进行了测定。并探讨了胆管癌细胞核形态学与其TNM分期、组织病理学分级、肿块大小、浸润性和淋巴转移等生物学行为的关系。研究结果表明:胆管癌细胞核内DNA含量和异型性的大小可作为判断其预后的客观指标;胆管癌肿块的大小与其癌细胞核内DNA含量成正比,且与DNA分布有关;胆管癌细胞核的异型性和DNA分布为探讨不同性别患者的胆管癌发病率提供了一个线索;胆管癌细胞核DNA倍体与其术后生存率的关系尚需进一步研究。 相似文献
16.
目的 应用脉冲电磁场 (PEMFs)刺激体外分离培养的人骨髓间充质干细胞 (hMSCs) ,对其生物学特性进行研究。方法 PEMFs (1 2Hz、 1 1mT、 8h/d)刺激hMSCs体外培养 ,计算细胞的倍增时间 ,观察细胞超微结构 ,放免法测定骨钙素的分泌情况和钙化结节VonKossa染色。结果 脉冲电磁场作用后的细胞增殖能力提高 ,细胞形态发生变化 ,透射电镜观察提示细胞成熟 ,VonKossa钙染色阳性 ,骨钙素的分泌活性增高。结论 hMSCs经合理的PEMFs体外刺激培养后 ,不仅能够大量扩增细胞 ,同时能够向成骨细胞转化 ,具有一定的成骨活性 ,是一种较为理想的骨种子细胞来源 相似文献
17.
18.
降钙素基因相关肽对鼠成骨细胞IGF—I及IGF—IFmRNA表达的影响 总被引:3,自引:4,他引:3
目的:探讨降钙素基因相关肽(CGRP)对成骨细胞胰岛素样生长固子I(IGF-I)和胰岛素样生长因子Ⅰ型受体(IGF-IR)基因表达的影响。方法:将体外培养的鼠成骨细胞用不同浓度的CGRP分别处理8h和24h,通过半定量逆转录聚合酶链式反应(RT-PCR)来观察成骨细胞二种目的基因(IGF-I,IGF-IR)的mRNA表达强度变化,结果:CGRP可以刺激成骨细胞IGF-IFmRNA表达,以10^-8M作用8h最为明显。CGRP亦能够上调成骨细胞IGF-IFmRNA表达,而且维持时间达24h。结论:CGRP通过增强IGF-I的自分泌作用来间接地调节成骨细胞活性,从而发挥成骨效应。 相似文献
19.
神经肽对人成骨细胞生物学影响机理的研究 总被引:5,自引:2,他引:3
目的:研究神经肽类物质对成骨细胞的钙离子通道及胞间通讯的影响。探讨其对细胞生物学特征影响的机制。方法:借助CLSM及Fluo-3/AMt CFDA荧光染色,观察0.1ng/ml的SP、NPY对成骨细胞内Ca^2 浓度及胞间通讯的影响。结果:借助于CLSM观察到SP、NPY及BMP均使成骨细胞的胞内Ca^2 浓度增加,并可加强细胞间的通讯联系。结论:神经肽引起胞间通讯的加强可使成骨细胞对各种因子刺激的反应更趋一致性,可提高成骨的质量。 相似文献
20.
目的 检测氟离子植入猪骨来源羟基磷灰石(PHA)对小鼠前成骨细胞(MC3T3-E1)黏附、 增殖及成骨分化的影响。方法 取猪骨松质用高温烧结的方法制备PHA,使用氟化钠浸泡结合二次高温 烧结进行氟离子植入,制备获得氟化猪骨源羟基磷灰石(FPHA)骨块,进行研磨获得颗粒材料后制备 压片,采用PHA颗粒制备的压片为对照组,采用FPHA颗粒制备的压片为实验组。在压片材料表面接种 小鼠MC3T3-E1前成骨细胞株,通过扫描电镜SEM观察不同时间细胞黏附形态,CCK-8法检测细胞增 殖情况,RT-PCR法检测细胞成骨相关基因碱性磷酸酶ALP、骨钙素BGLAP、骨桥蛋白OPN mRNA 的表达情况。结果 扫描电镜SEM观察可见,PHA与FPHA组细胞黏附生长良好;两组细胞接种1、7d 后OD值比较,差异无统计学意义(P>0.05);FPHA组接种3、5 d后OD值高于PHA组,差异有统计学 意义(P<0.05);RT-PCR结果显示,FPHA组细胞接种3、7 d后ALP、BGLAP mRNA表达水平均高于 PHA组,且两组细胞接种7 d后材料表面细胞ALP与BGLAP mRNA表达水平均高于细胞接种3 d后,差异 有统计学意义(P<0.05)。结论 PHA与FPHA材料均具有良好的细胞相容性,氟离子植入猪骨羟基磷灰 石可促进小鼠前成骨细胞早期增殖与成骨分化。 相似文献