抗rHIV—env1肽单克隆抗体杂交瘤细胞系的建立及初步鉴定

用rHIV-env_1肽免疫BALB/c小鼠脾细胞与小鼠骨髓瘤NS-1细胞融合,经4次克隆化获5株杂交瘤细胞系,其分泌抗体类型均属IgM。经连续传代6个月及液氮冻存9个月,分泌抗体特性无明显变化。5株McAb对rHIV-env_1肽或HIV-1均能明显地识别。用ELISA间接法测定各株McAb的相对亲和力,有3株(F7、B10、D6)McAb的50％最大结合浓度介于0.9～5μg/ml之间,用已如HIV-gp120抗体阳性血清阻断4株McAb与相应抗原的结合试验,所获结果各异。其中McAb B10与rHIV-env_1肽抗原的结合均可被4份HIV-gp120抗体阳性血清明显阻断,故初步认为B10McAb与抗HIV-gp120抗体中的保守肽段具有共同成分,抗HIV-env_1肽高度保守肽段的McAb,对艾滋病(AIDS)患者和人类免疫缺陷病毒(HIV)感染者的诊断具有实际应用价值。     

抗慢性B淋巴细胞白血病患者IgM单抗相对亲和力的分析

本文采用间接ELISA法检测了11株抗B-CLL患者IgM腹水和杂交瘤上清McAb的相对亲和力。结果提示,各株McAb结合抗原的相对亲和力不同。根据50％最大结合浓度分为两组。其中4株杂交瘤上清McAb与纯化腹水McAb测得结果基本吻合。实验结果为合理地选用这些单抗提供了重要依据。     

单克隆抗体亲和层析纯化绿脓杆菌外毒素A

本文采用ELISA法测定了本室研制的4株单抗的相对亲和力。当包被抗原浓度选定为5μg/ml后,饱和50％这一抗原浓度所需的抗体蛋白质量是:14F10为0.015μg/ml,23B9为0.010μg/ml,24B5为0.4μg/ml,23C12为2.5μg/ml。故它们的相对亲和力是:23B9>14F10>24B5>23C12。 选用23B9与CNBr一活化的Sepharose4B按说明书条件进行偶联,制备免疫吸附柱。将预处理的DE-52离子交换纤维素投入绿脓杆菌标准产毒株PA-103株培养物上     

抗rhG-CSF单克隆抗体的制备和初步鉴定

<正> 粒细胞集落刺激因子(G-CSF)是一种造血调控因子,为鉴定、检测和纯化人G-CSF,制备了四株特异识别人G—CSF的单克隆抗体(单抗,McAb)。1 材料和方法1.1 rhG-CSF和rhG-CSF融合蛋白 均由本实验室克隆、表达和纯化。纯化后的样品经SDS—PAGE鉴定,纯度大于95％。1.2 单抗制备 以rhG-CSF融合蛋白为抗原,按常规方法制备单抗。1.3 单抗识别抗原表位鉴定试验 用15μg/ml的抗原包被检测板(50μl/孔),对杂交瘤细胞的培养上清及其系列倍比稀释液进行间接ELISA检测,找出使抗原饱含的各McAb的最大稀释度。     

单克隆抗体对HFRS病毒两种粗制抗原相对亲和力的测定

McAb为传染病的病原研究和临床诊断等提供了新的手段和制剂。由于每一株杂交瘤分泌的McAb的特性可能各不相同,而其中McAb对相应抗原的亲和力高低是决定其用途的重要依据之一,因此有必要及早确定。经典的测定抗体亲和力方法一般是以抗原抗体反应的平衡常数K值表示,但K值的求得需对抗原抗体进行纯化、定量,方法烦琐,不能常规使用。本文采用ELISA法测定,虽不十分精确,但操作简便,无需特殊试剂和设备,能快速而明确地比较出各株McAb对病毒抗原亲和力的相对高低。 测定了32株抗肾综合征出血热(HFRS)     

用基因工程表达的HBcAg制备抗-HBcAg单克隆抗体及其初步应用

用基因工程表达的NBcAg免疫BALB/c小鼠,取脾细胞与小鼠骨髓瘤细胞Sp2/0融合,以ELISA抑制法检测抗体,获得28株分泌抗-HBc McAb的杂交瘤细胞系。对其中7株进行了克隆化,培养上清抗体滴度1:320～1:2560,免疫小鼠腹水和血清滴度1:10万～1:80万。Ig类型测定2株IgG1、2株IgG2a、1株IgG3、2株IgM。杂交瘤细胞系在体外连续传代培养6个月及液氮冻存的细胞系经复苏后仍能稳定地分泌McAb。得到的McAb只与HBcAg反应且能被HBcAg特异阻断,证明其特异性高。用ELISA间接法测定了McAb的相对亲和力,从50％最大结合的浓度分析结果,7株McAb的相对亲和力为2E6>2F5>2B11>2C10>1A7>1B7>1H7,浓度范围为0.6～10μg/ml。2E6和2C10 2株McAb与HRP结合,用ELISA双抗休夹心法筛选出2F5、2B111和H7 3株McAb适合作为包被抗体。2F5与2E6-HRP配对,建立了快速McAb-ELIS抑制法测定患者血清中抗-HBc。用本法测定了222份临床患者血清标本,结果与“华美”试剂盒测定结果基本一致。     

抗MHCⅡ类抗原单克隆抗体人血清蛋白氨甲喋呤结合物的制备及活性研究

作者采用间接偶联法,以人血清白蛋白作为中间载体制成氨甲噪吟-人血清白蛋白-鼠抗人MHCⅡ类抗原单克隆抗体结合物。经体外活性检测,证实该结合物对靶细胞Raii的IC50为0.6μg/ml,对非靶细胞Molt 4的IC50为10μg/ml,游离McAb HI43能竞争抑制这种作用。而相同浓度的结合物对造血干细胞克隆无抑制效应。     

抗O型口蹄疫病毒单克隆抗体相对亲和力的测定

目的:对3株O型口蹄疫病毒单克隆抗体(4A9、4C3、9F12)的相对亲和力进行测定.方法:用纯化的O型口蹄疫病毒作为包被抗原建立一种检测方法,将3株单克隆抗体(4A9、4C3、9F12)制备腹水并纯化,并用建立的检测方法测定3株单克隆抗体(4A9、4C3、9F12)的相对亲和力.结果:检测方法中抗原、酶标抗体的最佳稀释度分别为1∶800和1∶10 000,单抗的相对亲和力分别为:4A9约在5.242 μg/ml(1∶600),4C3约在0.763 μg/ml(1∶5 000),9F12约在0.127 μg/ml(1∶40 000),即9F12＞4C3＞4A9.结论:用建立的ELISA方法测定了3株O型口蹄疫病毒单克隆抗体(4A9、4C3、9F12)的相对亲和力,将为进一步研究口蹄疫病毒而选取合适的单抗提供理论依据.     

抗白血病患者IgM同种型和独特型McAb识别抗原表位的分析

本文应用间接ELISA试验,混合ELISA夹心试验,ELISA抑制试验对抗B-CLL患者血清IgM同种型和独特型McAb的特异性和亲和力进行了分析。并采用ELISA双抗体相加试验,检测了McAb识别抗原的表位。试验结果表明,14株McAb中10株为抗独特型McAb,4株为抗同种型McAb。对ELISA双抗体相加试验测得结果经微机集群处理分析,可将4株抗同种型McAb分成2组,10株抗独特型McAb分成4组。ELISA相加试验结果与间接混合ELISA抑制试验结果一致。结果提示,14株McAb至少可以识别6个不同的抗原表位。     

肾综合征出血热病毒50kD结构蛋白的抗原位点分析

采用23株抗HFRS病毒McAb,以放射免疫沉淀及ELISA等试验,证实HFRS病毒的50kD蛋白为该病毒的核蛋白(NP)。用抗NP的McAb对经亲和层析纯化的NP以ELISA阻断试验进行抗原位点分析,结果表明该蛋白与一般有囊膜病毒的NP相比,有两点明显的不同。一是HFRS病毒的NP上不仅有组特异性抗原位点,而且有型特异性抗原位点,两者都至少分布在2个区域,这些抗原位点及其分布区域之间有部分重叠或比较靠近。二是HFRS病毒的NP上可能存在中和抗原及血凝抗原位点。     

Level of functioning of siblings and parents of probands of varying degrees of retardation

A further analysis of already published data supports the position that retardates of low ability level less frequently have retarded siblings, retarded parents, and parents low in occupational level than do retardates higher in ability level. The analysis supports the position that there are two types of retarded individuals, persons retarded as a result of gene or chromosomal anomalies, brain injury, etc., who more frequently occur in the lower-level retardate group, and persons whose retardation represents polygenic segregation, who more frequently occur in the higher-level group.     

局部注射辛伐他汀对骨质疏松大鼠股骨髁骨小梁的改建效应

背景：局部注射具有成骨作用的辛伐他汀,可显著增加骨质疏松大鼠股骨颈及股骨髁部的骨密度及力学强度,分析局部注射辛伐他汀对股骨髁骨小梁的影响。 目的：进一步研究骨质疏松大鼠股骨内局部注射辛伐他汀对股骨髁骨小梁的影响。为将辛伐他汀应用于临床骨质疏松局部治疗提供实验基础。 方法：18只雌性SD大鼠双侧卵巢切除后3个月,制备大鼠骨质疏松模型。实验大鼠随机数字表法均分为3组,分别在实验大鼠的右侧股骨髓腔内单次注射辛伐他汀溶液5 mg、10 mg,对照组单纯注射空白载体。分别在注射后1个月处死大鼠并取材。Micro-CT扫描并定量分析骨组织形态变化。 结果与结论：给药后1个月,Micro-CT扫描结果显示,辛伐他汀治疗组的骨微结构参数如骨皮质厚度、骨小梁密度及连接率明显优于对照组。说明疏松骨骼单次注射小剂量辛伐他汀可显著促进股骨髁部骨小梁改建,改善骨骼微结构,可为强化局部、防治骨质疏松骨折的新选择进一步提供实验基础。     

石斛属民族药用植物的分类及生药学研究

中药石斛早在《神农本草经》中就被列为上品,其应用历史悠久,具有养阴生津、补肾益气、润喉护嗓、活血明目、抗癌防老等功效。本文对国内外石斛属民族药用植物的分类及生药学研究进行了综述。     

膝关节骨性关节炎的关节镜治疗体会

目的我科自2001～2005年4月对59例67膝的骨性关节炎（0A），进行关节镜检查及镜下清理术。方法镜检：膝关节骨性关节炎伴不同程度滑膜炎，滑膜皱壁粘连纤维片，关节软骨不同程度破坏。镜下清除增生滑膜，松解粘连，去除剥脱软骨、修复关节面。结果随访2月～4年，平均1.5年，术后综合评估2月～1年，优良率94％；1～2年，优良率79.2％；2年以上优良率54.8％。结论关节镜对膝关节骨性关节炎诊断能提供了比较全面的资料，并对骨性关节炎早期有良好疗效，具有创伤小、恢复快、并发症少和重复治疗等优点。     

Anatomical study of distribution of valves of the cutaneous veins of adult''s limbs

Since the cutaneous veins of the four limbs have been used as autogenous grafts in the treatment of cardiovascular disorders, knowledge of distribution of the valves is increasingly required depending upon the use. In the gross anatomical study of distribution of valves of the trunci venae, there has been argument about locational relationships between the venous roots and the valves in the vicinity of the roots and the inter-valvular distance. However, there have been only few reports discussing detailed information about valves of the cutaneous veins of the four limbs. The authors observed patterns of distribution of the cutaneous venous valves of the four limbs of cadavers prepared for practice in anatomy. The following parts were excised from each cadaver: the cephalic, basilic, and the great saphenous veins, which originate from the acral venous network and flow into the proximal deep veins, and the venous roots communicating with these veins. An incision was made on each excised vein in the direction of the long axis under observation with a stereoscopic microscope, and the inter-valvular distance and the distance between the valve and the orifice of venous root in the vicinity of the valve were measured. The inter-valvular distance varied with type of the truncus venae, and it varied according to area even in the same truncus venae.     

Pathways of the realization of immunomodulating effects of neurotransmitters

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 115, N ^o 4, pp. 401–404, April, 1993     

Characterization of sterols of three digenetic trematodes of buffalo

Sterols of three digenetic trematodes were isolated and characterized by infrared and 1H nuclear magnetic resonance spectroscopies, and gas-liquid chromatography and gas chromatography-mass spectrometry. Sterols identified were cholesterol, cholestanol, 24-methylcholesterol, 24-methylcholestanol, 24-ethyl-22-dehydrocholesterol, 24-ethyl-22-dehydrocholestanol, 24-ethylcholesterol and 24-ethylcholestanol.     

Mechanisms of generation of slow components of bioelectric activity in diagnostics of functional state of the gastrointestinal tract

New electrophysiologic devices were used for the diagnostics of the state of excitable structures in the gastrointestinal tract and correction of their motor functions. Bioelectrical and biomechanical activities form the basis of functioning of internal organs. The mechanisms of generation of slow bioelectrical activity that are important for clinical and physiological studies are described. One of these mechanisms is a capacitance parametric transducer converting the energy of contractions into specific electric signals reflecting muscle functions. Another mechanism results from slow oscillations of resting potentials of interrelated excitable cells in large neuromuscular structures of internal organs. The elaborated procedure is efficient for preventing early postoperative paresis of the gastrointestinal tract. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 128, No. 10, pp. 448–452, October, 1999     

胃小弯侧血管弓的MDCT解剖

目的：研究正常胃小弯侧血管弓及其属支血管的多排螺旋CT表现。方法：回顾性分析51例正常成人的上腹部CT资料,观察胃小弯侧血管弓及其属支血管的显示情况、血管弓形态及其与胃壁的关系。结果：51例均显示胃小弯侧血管弓,血管弓贲门部属支、胃体部属支和幽门部属支分别显示46例、15例和10例;血管弓与胃壁紧邻41例。结论：MDCT检查可良好显示正常胃小弯侧血管弓及其属支血管。     

Assessment of the efficacy of treatment of hemangiomas

Assessment of the aftereffects of cryoexposure and ultrahigh-frequency cryoexposure on hemangioma tissue of various types, cavernous and squamous, showed a higher cryogenic effect in hemangiomatous tissue preexposed to ultrahigh-frequency waves. A quantitative criterion is proposed for assessing the efficacy of the studied methods of exposure. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 6, pp. 669–672, June, 1995