ELISA补体结合试验(COMPELISA)检测布鲁氏菌抗体的研究

我们参考Robertson氏介绍的方法,建立了ELISA补体结合试验(COMPELISA)、并以此方法检查了非布鲁氏菌病人血清120份、布鲁氏菌病人血清100份。结果表明此法敏感性比补体结合反应高得多,按Vecchio氏介绍的评价力法,COMPELISA试验诊断布鲁氏菌病的敏感性为96%,特异性100%。 此外,证实此试验结果的重现性较好。     

间接荧光抗体、补体结合和玻片絮状试验过筛血吸虫病的评价

为了进一步评定间接荧光抗体法(IFA)作为过筛试验的价值,并与补体结合(CF)、玻片絮状(SF)试验检查血吸虫病可疑对象的结果相比较,作者等对从海外血吸虫病流行区返回非流行区的工作人员、流行区的本地居民和长期居住者及部分经临床和寄生虫学方法诊断为血吸虫病的国外工作人员进行了检查。     

RESA-间接荧光抗体试验检测恶性疟RESA抗体的研究

由体外培养的恶性疟原虫获得的含环状体为主的感染红细胞制成单层感染红细胞,经戊二醛固定后空气干燥,作为RESA,抗原片,以RESA-IFA检测恶性疟患老血样中的RESA抗体。受检的58份恶性疟血样中,12份呈RESA抗体阳性反应,阳性率为20.7％,其中8份用IFA检测时抗体滴度均>1∶4096。对20份间日疟血样进行了同样试验,结果均为阴性。对RESA及其抗体在疟疾保护性免疫中的作用进行了简要讨论。     

检测阿米巴粪抗体的间接血凝试验

Shaalar和Baker曾用补体结合试验首先证实了对抗溶组织内阿米巴的粪抗体。后来的研究又表明间接血凝试验比补体结合试验有更高的敏感性和专一性。本文作者在间接血凝试验中使用戊二醛处理的羊红细胞检测肠胃道功能紊乱病人大便中阿米巴的粪抗     

滤纸干血滴间接荧光抗体试验检测利什曼抗体

作者采用滤纸干血滴对伊拉克儿童医院内的4,142例病人作了利什曼病的间接荧光抗体试验。方法是从病人指尖采血滴在滤纸,凉干后放入盛有少量矽胶的聚乙烯袋内,于一20oC保存。试验前剪取直径为smm的干血滴标本,放在滴加pH7 .2的pBS 0 .05。l的抗原片上,放在湿盒内1小时,用PBS洗去滤纸,然后再用PBS冲     

间接荧光抗体试验检测广州管圆线虫病

目的 研究温州流行性嗜酸粒细胞增多性脑膜炎病是否为广州管圆线虫。方法 间接荧光标记抗体技术检验广州管圆线虫抗体,间接证实广州管圆线虫感染。结果 发现此批病人血清存在广州管圆线虫抗体,阳性检出率为８３％,且阳性滴度大于１：１０。结论 此批因食不熟福寿螺肉而患流行性嗜酸粒细胞增多性脑膜炎病人为广州管圆线虫感染引起。     

间接血凝法检测孕妇血清、阴道分泌物沙眼衣原体抗体

沙眼衣原体可以引起多个部位的感染，泌尿生殖道感染是一种常见的性传播疾病，间接血凝法检测孕妇血清中的沙眼衣原体抗体能否反应孕妇泌尿生殖道的感染情况，是一个有待于解决的问题。孕妇沙眼衣原体感染对孕妇和新生儿健康的危害都比较严重，了解孕妇沙眼衣原体的感染情况可为制订预防措施提供理论依据。１　材料和方法１１　研究对象和标本采集　对４０２例于１９９５年３～１２月间每周２在山东医科大学附属医院妇产科进行产前检查的孕妇采上臂静脉血３ｍｌ，分离血清。１１４例于１９９５年４～６月间每周２就诊的孕妇采集阴道分泌物…     

间接免疫荧光试验在西尼罗病毒抗体检测中的初步应用

目的了解人群血清西尼罗病毒(WNV)抗体存在的本底,探讨WNV与乙型脑炎病毒(JEV)的免疫交叉反应。方法以新兵入伍健康体检时收集的男性血清347份为检测标本,应用建立的间接免疫荧光试验(IFA)对WNV与JEV的IgG抗体进行检测。结果血清WNV和JEV的抗体阳性率分别为3.2%(11/347)和5.7%(14/244);在同时检测了两种病毒抗体的244份标本中,有3份两者均为阳性,分别占WNV和JEV抗体阳性数的60%(3/5)和21%(3/14)。结论人群对WNV缺乏免疫力,JEV抗体对WNV的交叉免疫作用有一定的局限性。     

间接荧光抗体试验检测广州管圆线虫病患者血清抗体

广州管圆线虫（Ａｎｇｉｏｓｔｒｏｎｇｙｌｕｓｃａｎｔｏｎｅｎｓｉｓ）成虫主要寄生在鼠体的肺部血管,人体因生食含有广州管圆线虫第三期幼虫（感染期幼虫）的软体动物等方式而获得感染,幼虫常侵入人体中枢神经系统而致嗜酸性粒细胞增多性脑膜脑炎和脑脊髓膜炎（酸脑）。近年来我国大陆广东、上海、福建等地亦有人体病例或鼠体感染发现［１－３］。１９９７年１０～１１月,温州市部分居民因生吃含广州管圆线虫的福寿螺而出现爆发性“酸脑”多起。试用间接荧光抗体试验（ＩＦＡＴ）检测成虫粗抗原皮试［４］阳性的３２例患者血清,以探…     

间接免疫荧光试验与补体结合试验诊断人体旋毛虫病的效果比较

作者于1981—1983年间,用间接免疫荧光试验(IIF)及补体结合试验(CFC)检查了立陶宛苏维埃共和国不同地区623人的645     

The Use of Complement Fixation Tests to Detect Australia Antigen-Antibody Complexes and Antibodies to a Tween Antigen1

Abstract. Indications were obtained that Australia antigen-antibody complexes are more frequent in patients with cirrhosis than in other Australia antigen-positive individuals. The evidences were: (1) Among sera with high titres of Australia antigen, anticomplementary activity was detected at lower dilutions in 53% of the cases of cirrhosis, as compared to 14 and 7% in hepatitis patients and carriers, respectively. (2) Among anticomplementary sera which did not show Australia antigen at higher dilutions, the antigen was unmasked after heating at 85 °C in 4 of 7 cases of cirrhosis and in 2 of 11 other patients. On the other hand, a new type of antibody was demonstrated in three patients who had experienced a progressive decrease of Australia antigen titre; the antibodies were detected after the disappearance of the antigen, but they reacted only with some antigenic component of Australia-positive sera which was unmasked after treatment with Tween 80.     

Indirect ELISA Using Multi–Antigenic Dominants of p30, p54 and p72 Recombinant Proteins to Detect Antibodies against African Swine Fever Virus in Pigs

African swine fever (ASF) caused by ASF virus (ASFV) is a fatal disease in pigs and results in great economic losses. Due to the lack of available vaccines and treatments, serological diagnosis of ASF plays a key role in the surveillance program, but due to the lack of knowledge and the complexity of the ASFV genome, the candidate target viral proteins are still being researched. False negativity is still a big obstacle during the diagnostic process. In this study, the high antigenic viral proteins p30, p54 and p72 were screened to find the antigenic dominant domains and the tandem His–p30–54–72 was derived. An indirect enzyme–linked immunosorbent assay (iELISA) coated with His–p30–54–72 was developed with a cut–off value of 0.371. A total of 192 clinical samples were detected by His–p30–54–72–coated indirect ELISA (iELISA) and commercial ASFV antibody kits. The results showed that the positive rate of His–p30–54–72–coated iELISA was increased by 4.7% and 14.6% compared with a single viral protein–based commercial ASFV antibody kits. These results provide a platform for future ASFV clinical diagnosis and vaccine immune effect evaluation.     

In Vitro Diagnostic Assay to Detect SARS-CoV-2-Neutralizing Antibody in Patient Sera Using Engineered ACE-2 Mini-Protein

The recent development and mass administration of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) vaccines allowed for disease control, reducing hospitalizations and mortality. Most of these vaccines target the SARS-CoV-2 Spike (S) protein antigens, culminating with the production of neutralizing antibodies (NAbs) that disrupt the attachment of the virus to ACE2 receptors on the host cells. However, several studies demonstrated that the NAbs typically rise within a few weeks after vaccination but quickly reduce months later. Thus, multiple booster administration is recommended, leading to vaccination hesitancy in many populations. Detecting serum anti-SARS-CoV-2 NAbs can instruct patients and healthcare providers on correct booster strategies. Several in vitro diagnostics kits are available; however, their high cost impairs the mass NAbs diagnostic testing. Recently, we engineered an ACE2 mimetic that interacts with the Receptor Binding Domain (RBD) of the SARS-2 S protein. Here we present the use of this engineered mini-protein (p-deface2 mut) to develop a detection assay to measure NAbs in patient sera using a competitive ELISA assay. Serum samples from twenty-one patients were tested. Nine samples (42.8%) tested positive, and twelve (57.1%) tested negative for neutralizing sera. The data correlated with the result from the standard commercial assay that uses human ACE2 protein. This confirmed that p-deface2 mut could replace human ACE2 in ELISA assays. Using bacterially expressed p-deface2 mut protein is cost-effective and may allow mass SARS-CoV-2 NAbs detection, especially in low-income countries where economical diagnostic testing is crucial. Such information will help providers decide when a booster is required, reducing risks of reinfection and preventing the administration before it is medically necessary.     

以重组蛋白为抗原的ELISA法检测旋毛虫抗体

目的 寻求特异性强、敏感性高的旋毛虫病诊断抗原。 方法 以旋毛虫新生幼虫期特异性T668基因在E.coli高效表达的重组蛋白为抗原，分别以兔、猪和健康者血清及旋毛虫病阳性血清为一抗，以辣根过氧化物酶（HRP）标记的山羊抗兔IgG、山羊抗猪IgG和山羊抗人IgG为二抗，建立检测旋毛虫抗体的间接ELISA方法，并以旋毛虫肌幼虫排泄?鄄分泌（ES）抗原作为检测对照。 结果 以T668重组蛋白为抗原，对兔、猪和人旋毛虫病血清进行检测，阳性检出率为100 ％，且敏感性高（0.016 μg / 孔），与ES抗原检测结果完全一致。 结论 T668重组抗原有望替代ES抗原检测旋毛虫抗体。     

An Enzyme-Linked Antiglobulin Test to Detect Red Cell Globulins after Glutaraldehyde Fixation

The purpose of this study was to develop an enzyme-linked antiglobulin test (ELAT) for IgG on RBC without the hemolysis caused by the high pH of the alkaline phosphatase reaction. This was achieved by fixing the RBC with 0.05% glutaraldehyde after attachment of the antibodies. Assays using anti-D reference standards demonstrated the sensitivity to be 1-2 ng of antibody as compared to 7.5 ng for the manual indirect antiglobulin test. The coefficients of variation of these assays ranged from 10.4 to 20.1%. The mean background absorbance at 405 nm of 105 normal RBC samples was 0.08 +/- 0.03 SD. There was an increase in sensitivity of the test after the fixed RBC were stored. Dilute glutaraldehyde stabilizes the RBC membrane and the antigen-antibody linkage resulting in a more sensitive ELAT.