三氯乙烯药疹样皮炎患者治疗前后肝功能与外周血淋巴细胞亚群变化相关分析

目的 研究三氯乙烯药疹样皮炎患者治疗前后肝功能与外周血淋巴细胞亚群的变化特点,探索两者之间的联系,为该病发病机制的研究提供线索。 方法 采用双标法在流式细胞仪上对14例确诊为三氯乙烯药疹样皮炎的患者(其中9例为剥脱性皮炎、5例为多形红斑)在入院时及治疗2~3月后(停用糖皮质激素)进行外周血淋巴细胞亚群检测及肝功能指标检测,比较治疗前后肝功能与淋巴细胞亚群的变化情况。 结果 与正常参考值相比,治疗前患者均存在不同程度肝损伤,治疗后患者肝功能基本恢复正常。患者治疗前后的CD3+细胞、CD8+T细胞(CD3+CD8+)百分数均高于正常参考值,差异有统计学意义(t=2.283~6.841,P < 0.05或0.01);而CD4+T细胞(CD3+CD4+)、NK细胞[CD3-CD (16+56)+]、B淋巴细胞(CD3-CD19+)百分数、CD4+/CD8+比值均低于正常参考值,差异有统计学意义(t或Z=-14.49~-2.310,P < 0.05或0.01)。与治疗前比较,剥脱性皮炎患者治疗后的CD8+T淋巴细胞、B淋巴细胞百分数降低,趋于正常水平,差异有统计学意义(t=2.814、2.877,P < 0.05);其他指标在治疗前后比较,差异无统计学意义(P>0.05)。多形红斑患者各指标在治疗前后比较,差异均无统计学差异(P>0.05)。与入院时相比,免疫球蛋白M (IgM)、免疫球蛋白A (IgA)水平下降,差异有统计学意义(t=2.256、2.293,P < 0.05);免疫球蛋白G (IgG)水平在治疗前后比较,差异无统计学意义(t=1.783,P>0.05)。治疗前谷酰转肽酶水平与成熟T淋巴细胞(CD3+)、CD8+T细胞百分数均存在正相关关系(r=0.496、0.470,P < 0.05);胆碱酯酶、白蛋白等指标与成熟T淋巴细胞、CD8+T细胞百分数均存在负相关关系(r=-0.567、-0.475、-0.501,-0.516,P < 0.05)。 结论 三氯乙烯药疹样皮炎患者在较长的时间内存在免疫功能紊乱,三氯乙烯药疹样皮炎可能为细胞免疫与体液免疫共同作用的结果,CD8+T细胞的增殖可能与其肝脏和皮肤损害的机制有关。     

Increase in Memory (CD4+CD29+ and CD4+CD45RO+) T and Naive (CD4+CD45RA+)T-Cell Subpopulations in Smokers

To examine the effects of smoking on lymphocyte subpopulations, we measured the following cell subpopulations: CD4+ T-cell subpopulations (i.e., CD4+CD29+, CD4+ CD45RO+, and CD4+CD45RA+ cells); CD8+ T-cell subpopulations (i.e., CD8+CD11a+ and CD8+CD11b+ cells); and natural killer cell subpopulations (i.e., CD16+CD57-, CD16+CD57+, and CD16-CD57+ cells). We measured these subpopulations, together with total CD4+ T, total CD8+T, total CD3+T, B (CD19+), and total lymphocytes, in 10 male heavy smokers, 38 male light-to-moderate smokers, and 33 male nonsmokers. The mean ages were 30 y, 31 y, and 32 y, respectively, and ages did not vary significantly among the smokers. CD4+CD29+ and CD4+CD45RO+ (memory T) cells in heavy smokers were significantly more numerous than those in light-to-moderate smokers and nonsmokers. Also, these memory T-cell subpopulations were significantly more numerous in light-to-moderate smokers than in non-smokers. The number of CD4+CD45RA+ (naive T) cells was significantly larger in heavy smokers than nonsmokers; numbers of CD4+CD45RO+ T and CD4+CD29+ T cells (memory T cells) were significantly correlated with daily cigarette consumption. Numbers of CD3+ T, CD4+ T, CD19+ B, and total lymphocytes in heavy smokers were significantly larger than in nonsmokers. There were significantly more CD3+ T, CD4+ T, and total lymphocytes in light-to-moderate smokers than in nonsmokers. The numbers of CD4+ T lymphocytes in heavy smokers were significantly larger than in light-to-moderate smokers. Perhaps CD4+ T cell subpopulations, especially memory T cells, are most susceptible to the effects of smoking on lymphocyte sub-populations.     

Repeated high-intensity Wingate cycle bouts influence markers of lymphocyte migration but not apoptosis

Studies have shown significant changes in lymphocytes during continuous exercise, but little has been shown on the effect of repeated high intensity bouts. This study was designed to examine the effect of repeated intermittent bouts on lymphocyte subset cell count, apoptosis, and migration. A series of 6 Wingate anaerobic cycle tests were performed by participants (N = 8) with blood samples attained before, immediately following, and after a designated recovery period (excess postexercise oxygen consumption (EPOC)) to observe lymphocyte changes. Lymphocyte subsets (CD4+, CD4/CD45RA+, CD8+, CD8+/CD45RA+, CD19+) were assessed for apoptosis (annexin V+) and cellular migration (CX(3)CR1). Our results indicate that the CD8+ and CD8+/CD45RA+ subsets were significantly influenced by the repetitive Wingate cycling protocol such that cell counts increased with exercise, and then decreased at EPOC termination (p = 0.016). The observed postexercise decrease in CD8+ and CD8+/CD45RA+ cells was accompanied by a significant change in the CX(3)CR1 cell migration receptor (p = 0.019), but not apoptosis (p = 0.87). This indicates that with repetitive high-intensity cycling, the response in CD8+ cells following the bout is likely due to cell migration rather than cell death.     

河南地区健康儿童淋巴细胞亚群参考区间的建立

目的建立适合河南地区年龄、性别相关的健康儿童淋巴细胞亚群参考区间。方法采用多阶段分层整群随机抽样的方法,抽取河南省5个地市1 027名<7岁健康儿童,分婴儿组(4~12月龄,n=45)、幼儿组(1~3岁,n=190)和学龄前组(3~7岁,n=792)进行研究。用流式细胞术双平台法检测淋巴细胞亚群百分比和绝对计数。结果学龄前组不同性别健康儿童之间,淋巴细胞免疫亚群CD3^+、CD3^+CD4^+、CD3^+CD8^+、CD4^+/CD8^+和CD3^+CD16^+CD56^+百分比差异均有统计学意义(P值均<0.01),绝对计数CD3^+CD4^+、CD3^+CD8^+、CD3^+CD16^+CD56^+差异均有统计学意义(P值均<0.01);不同年龄组健康儿童之间淋巴细胞亚群百分比和绝对计数差异均有统计学意义(P值均<0.01)。与国内已发表的淋巴细胞亚群百分比相关文献比较,相同年龄段淋巴细胞亚群相对计数参考区间明显不同。结论年龄、性别影响健康儿童淋巴细胞亚群的分布,本研究可为建立河南省健康学龄前儿童年龄和性别相关的淋巴细胞亚群参考区间提供依据。     

Altered partition of t cell subsets in the peripheral blood of healthy workers exposed to flour dust

Occupational exposure to organic compounds can induce obvious immunological disorders or more subtle modifications. We investigated peripheral blood lymphocyte subsets in 34 bakers and 82 millers exposed to wheat flour dust, and 51 salt factory workers. Significantly decreased levels of CD4 +, CD8 +, CD57 + and CD8 + /57 + cells were noted in mill workers, and of CD57+ cells in bakers. CD29 + and CD4 + /CD29 + cells were significantly lower in millers, CD4 + /CD45RA + cells higher in all exposed workers. The lower numbers of positive cells noted in millers appeared associated to significantly higher (p<0.001) levels of CD29 and CD45RA expression as measured by fluorescence intensity. These data are opposite to those previously reported in asthmatic workers exposed to flour dust. Since the individuals tested here were clinically healthy, the alterations of T-cell subsets observed could be interpreted as a successful attempt at immunoregulation maintaining homeostasis.     

Absolute counts and distribution of lymphocyte subsets in small intestine of BALB/c mice change during weaning

The gut immune system is an essential part of the barrier function of the gut. At weaning, major changes can be expected in the number and subset composition of lymphocytes in the small intestine since the gut is exposed to a wide variety of food and microbial antigens, especially when human milk is gradually replaced by weaning foods. The purpose of this study was to evaluate the changes in small intestine lymphocyte subsets in mice during weaning. BALB/c male mice at weaning (3 wk old) were fed a nonpurified diet for 18 d and were killed at different times (0, 4, 7, 12 and 18 d). Lymphocyte populations from lamina propria (LPL), Peyer's patches (PPL) and intestinal epithelium (IEL) were isolated. The expression of different antigens (CD3, CD4, CD8alpha, CD8beta, CD22 and CD45R) in those lymphocyte populations was analyzed by flow cytometry. The percentages of cells expressing T-cell antigens, such as CD3, were significantly higher in LPL during weaning compared to d 0. The percentages of cells expressing CD8alpha and CD8beta increased in both IEL and LPL. However, the percentage of CD4+ cells tended (P = 0.07) to decrease in IEL and to increase in LPL. The percentages of cells expressing B-cell antigens, such as CD22 or CD45R in PPL increased. Changes in the specific phenotypes of intestinal lymphocyte populations at weaning are apparently related to the maturation of the intestinal immune system during early life. Thus, B cells increase in PPL and T cell increase in IEL and LPL.     

Long-term effects of perinatal nutrition on T lymphocyte kinetics in young Gambian men

BACKGROUND: Nutritional status is highly dependent on season in countries such as The Gambia. In a rural Gambian setting, individuals born during periods of seasonal nutritional deprivation ("hungry seasons") are susceptible to mortality from infectious diseases in adult life. OBJECTIVE: We investigated the hypothesis that impaired immunocompetence in those born in the hungry season results from an underlying defect in immunologic memory, similar to the immunosenescence of old age, which is likely to be reflected in the phenotype and kinetics of T lymphocytes in young adults. DESIGN: T cell phenotype in terms of CD3, CD4, CD8, CD45RA, and CD45R0 expression and in vivo dynamics measured by stable isotope labeling of T cell subsets combined with gas chromatography-mass spectrometry and frequency of T cell receptor excision circles were measured in 25 young (18-24-y-old) Gambian men. Thirteen of these 25 men were exposed to perinatal malnutrition as defined by birth season and birth weight. RESULTS: In persons born in the hungry season with low birth weight, no differences in the proportions of memory or naive T cells were found. Kinetic analysis showed higher proliferation rates in memory (CD45R0(+)) subsets of T cells than in na?ve (CD45R0(-)) cells, which is consistent with previous studies, but no evidence was found for an effect of birth weight or season on T lymphocyte proliferation and disappearance rates. No significant correlations were found between in vivo T cell kinetics and frequency of T cell receptor excision circles. Only absolute numbers of granulocytes were elevated in those born in the nutritionally deprived season. CONCLUSION: In healthy young Gambian men, T lymphocyte homeostasis is extremely robust regardless of perinatal nutritional compromise.     

肥胖儿童T淋巴细胞亚群的功能评估

目的了解肥胖儿童的细胞免疫功能状态。方法2002年4月～2003年10月,共采集我院肥胖专科门诊儿童和青少年的单纯性肥胖患儿47例,其中男30例,女17例,平均年龄(11.4±2.2)岁;同期随机抽取37名正常体重学生的健康自愿者作为对照组,其中男16例,女21例,平均年龄(13.4±1.0)岁。采集肘部静脉血,采用流式细胞仪进行T淋巴细胞的亚群分析。结果肥胖组的T淋巴细胞亚群CD3+、CD4+、CD8+和CD4+/CD8+与对照组相比,差异均无显著性(均P>0.05);且CD3+、CD4+、CD8+和CD4+/CD8+与肥胖程度无相关性(r分别为-0.012、-0.102、0.139、-0.103,均P>0.1)。正常组CD3+、CD4+、CD8+和CD4+/CD8+与体重也无相关性(r分别为-0.223、-0.076、-0.190、-0.072,均P>0.1)。结论肥胖患儿T淋巴细胞亚群的免疫功能无减退现象。     

特发性血小板减少性紫癜患者外周血淋巴细胞亚群和血小板特异性抗体检测的临床意义

目的 检测特发性血小板减少性紫癜(ITP)患者外周血淋巴细胞亚群、血小板膜糖蛋白(GPⅡb/Ⅲa、GPⅠb/Ⅸ)特异性抗体变化,探讨相关因素在ITP发病机制中的作用.方法 用流式细胞术检测72例ITP患者(ITP组)和50例健康体检者(对照组)外周血淋巴细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+、CD19+变化.应用改良血小板抗原单克隆抗体固相化检测技术测定GPⅡb/Ⅲa、GPⅠb/Ⅸ特异性抗体变化.结果 ITP组与对照组比较,GPⅡb/Ⅲa、GP Ⅰ b/Ⅸ特异性抗体吸光度值升高(P＜0.05),CD3+、CD4+、CD4+/CD8+显著下降(P＜0.01),CD8+、CD19+显著增高(P＜0.01).结论 淋巴细胞亚群及血小板特异性抗体可较好地反映ITP的病理机制,在ITP的发病机制中起非常重要的作用.     

传染性单核细胞增多症患儿血浆sHLA-G及外周血淋巴细胞亚群检测

目的 通过检测EB病毒(EBV)感染传染性单核细胞增多症(IM)患儿的血浆可溶性HLA-G (sHLA-G)水平及外周血淋巴细胞亚群比例,分析EBV感染IM患儿血浆sHLA-G水平与外周血淋巴细胞亚群的相关性.方法 采用酶联免疫吸附试验(ELISA),检测了51例EBV感染IM患儿及146例正常对照儿童血浆sHLA-G水平,采用流式细胞术检测了86例EBV感染IM患儿及30例正常对照儿童的外周血淋巴细胞亚群.结果 EBV感染IM组血浆sHLA-G水平130.30(77.28～217.23)U/ml,明显高于对照组的19.82(6.39～44.90) U/ml,经Mann-Whitney U检验差异有统计学意义(Z=-9.472,P＜0.01);EBV感染IM组外周血CD3+、CD8+淋巴细胞百分率较对照组明显上升、而CD4+、CD4 +/CD8+、CD16+56+、CD19+淋巴细胞百分率较对照组明显下降,差异均有统计学意义(均P＜0.01);EBV感染IM组血浆sHLA-G水平与CD3+、CD4+、CD8+、CD4 +/CD8+、CD16+56+以及CD19+细胞表达率间均无明显相关性.结论 EBV感染IM患儿血浆sHLA-G水平明显升高,并存在细胞免疫功能紊乱,血浆sHLA-G水平可作为EBV感染IM的辅助诊断指标,其升高的机制还有待于更深入的研究.     

褪黑素对鞘内泵入吗啡致免疫功能抑制大鼠免疫功能的影响

目的本实验采用ALZET泵建立鞘内持续泵入吗啡所致的免疫功能抑制大鼠模型,腹腔注射褪黑素观察其对鞘内泵入吗啡大鼠免疫功能的影响。方法 32只SD大鼠随机分为4组：生理盐水组（NS组,n=8）、吗啡组（M组,10μg/h,n=8）,吗啡组＋褪黑素组（M＋MLT组,n=8）,吗啡组＋生理盐水组（M＋NS组,n=8）。用microspinal导管根据改良Yaksh法进行鞘内置管,连接ALZET泵,持续泵入吗啡、生理盐水,MLT组连续7 d腹腔注射褪黑素,每次时间点为7：00-9：00 am,5 mg/kg。M＋NS组连续7 d腹腔注射生理盐水。7 d后原代分离脾脏单个核细胞进行培养,采用3H-TDR（甲基3H胸腺嘧啶核苷）掺入法检测脾T淋巴细胞对刀豆蛋白（ConA）刺激诱导的增殖水平;乳酸脱氢酶（LDH）释放法检测脾NK细胞活性;流式细胞仪检测脾T淋巴细胞亚群（CD3＋、CD3＋CD4＋、CD3＋CD8＋、CD4＋/CD8＋）和NK细胞（CD161＋）表型变化。结果与NS组相比较,泵入吗啡7 d后M组T淋巴细胞增殖水平、NK细胞活性降低（P〈0.05）,M组CD3＋、CD3＋CD4＋、CD3＋CD8＋细胞数量及百分率降低,CD4＋/CD8＋降低,CD161＋降低（P〈0.05）;同M＋NS组比较,M＋MLT组T淋巴细胞增殖水平、NK细胞活性升高（P〈0.05）,M＋MLT组CD3＋、CD3＋CD4＋、CD3＋CD8＋数量及百分率升高,CD4＋/CD8＋升高,CD161＋升高（P〈0.05）。结论鞘内泵入吗啡（10μg/h）可明显抑制大鼠细胞免疫功能;褪黑素可改善鞘内泵入吗啡所致的免疫功能抑制大鼠细胞免疫功能。     

Flow cytometric analysis of lymphocyte subpopulations in the spleen and thymus of mice exposed to an acute immunosuppressive dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

The objective of the present studies was to determine if acute exposure to an immunotoxic dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) produces alterations in the expression of lymphocyte surface markers as measured by multiparameter flow cytometry. The immunotoxicity of a single oral dose of TCDD was assessed by the anti-SRBC PFC response; an ED50 of 0.74 micrograms/kg was determined. Subpopulations in the spleen and thymus of C57B1/6 mice were analyzed 2 days following exposure to 2 micrograms/kg TCDD. In addition, splenic lymphocyte subsets were examined on Days 1-4 following SRBC challenge of mice treated with 0, 2, or 5 micrograms/kg TCDD. T and B cells were identified by single parameter analysis of Thy 1.2 and Ig expression. T cell subsets were defined by dual parameter analysis of CD4 and CD8 expression. In TCDD-treated mice, the percentage and the total number of double-positive CD4+ CD8+ thymocytes were significantly decreased while the percentage but not the total number of double-negative CD4- CD8- thymocytes was significantly increased. No changes in the percentage or total number of single positive (CD4+ CD8- or CD4- CD8+) thymocyte subsets were observed. In contrast to the thymus, lymphocyte subsets in the spleen were not significantly altered in percentage or total number 2 days following acute TCDD exposure. When splenic lymphocytes were analyzed daily following SRBC challenge, Ig+, Thy 1.2+, and CD4+ CD8- subpopulations remained relatively unchanged in both control and TCDD-treated animals. A small but significant decrease in the percentage of CD4- CD8+ T cells was observed on Day 3 in mice treated with 2 or 5 micrograms/kg TCDD when compared to that of vehicle-treated mice. The total number of CD4- CD8+ splenocytes was also significantly lower in the 5-micrograms/kg group on Day 3. However, this effect appeared to result from an elevation of the CD4- CD8+ subset in the controls rather than from a reduction in the TCDD-treated groups. Double-positive (CD4+ CD8+) lymphocytes were not detected in either control or TCDD-treated spleens. These results indicate that an acute dose of TCDD which reduced the splenic anti-SRBC response by 65-80% did not cause detectable changes in major splenic lymphocyte subpopulations. This is an important finding from the standpoint of utilizing lymphocyte subset analysis to screen for potential immunotoxic effects of TCDD. Specifically, the absence of subset changes does not preclude the presence of functional immunosuppression.     

铬盐职业接触对T细胞部分免疫功能的影响

目的 了解铬盐对职业接触者体内免疫指标变化的影响.方法 115名不同浓度铬盐接触工人为接触组,以远离铬盐污染区的90名居民为对照组.采用原子吸收分光光度法测定研究对象作业环境铬盐浓度;采用荧光标记流式细胞分析法测定研究对象外周血中T细胞CD3~+、CD3~+CD4~+、CD3~+CD8~+及CD4~+/CD8~+水平.结果 接触组空气中铬盐个体环境暴露浓度为(27.51±33.25)μg/m~3,对照组浓度为(0.16±0.15)μg/m~3,两组比较差异有统计学意义(z=8.045,P<0.01).铬盐接触组人群CD3~+、CD3~+CD4~+、CD3~+CD8~+及CD4~+/CD8~+平均水平分别为:(30.08±17.75)%、(1.04±1.73)%、(11.94±9.78)%、0.10±0.14;对照组为:(63.00±13.57)%、(30.51±5.16)%、(14.82±4.59)%、2.17±0.53.两组比较,对照组高于接触组,差异有统计学意义(z值分别为:4.484、5.227、1.976、-5.218,P值均<0.05).结论在铬盐个体环境暴露评价基础上,铬盐接触者以T细胞为主的细胞免疫功能受到抑制,而CD3~+CD4~+有望作为铬盐接触免疫损伤的早期效应性生物标志物,值得进行更深入研究.     

Distribution of naïve (CD45RA+) and memory (CD45RO+) T-cells in HIV-infected Puerto Rican population

HIV infection usually results in a gradual deterioration of the immune system. It is evident that early recognition of progression markers during HIV infection from asymptomatic to symptomatic state is needed. In the present cross-sectional study, peripheral blood lymphocytes from 63 HIV-infected Puerto Rican individuals were analyzed by two-color flow cytometry to study the co-expression CD45RA and CD45RO on both CD4+ and CD8+ T-cells and its correlation with age, gender, CD4 count, CD4:CD8 ratio, anti-retroviral therapy, clinical status, and viral load. Measurement of T-cell subsets in these patients showed an excessive increase of CD3+CD8+, CD8+CD45RA+, and CD8+CD45RO+ T-cells as disease progresses. In contrast, it was also observed a significant decrease in CD3+CD4+, CD4+CD45RA+ and CD4+CD45RO+ T-cells. The distribution of CD8+CD45RA+ T-cells did not change significantly between HIV and AIDS cases suggesting that this T-cell subset is not a good progression marker. Interestingly, CD4+CD45RA+ T-cells were significantly difference between genders, and CD44+CD45RA+ and CD8+CD45RO+ T-cells were influenced by age. In conclusion, the distribution of na?ve/memory CD4+ T-cells and memory CD8+ T-cells significantly correlate with HIV infection in disease progression. It is also important to mention that these T-cell subpopulations may be influenced by both gender and age. Overall, these results suggest that a loss in the generation of new immune response and function may be occurring during disease progression. This study open new windows of understanding that will be beneficial for future studies on immunopathogenesis, diagnosis, prognosis, and treatment monitoring for HIV/AIDS.     

Zinc-deficient rats have fewer recent thymic emigrant (CD90+) T lymphocytes in spleen and blood

It has been hypothesized that increased expression of the signaling protein p56(lck) disrupts maturation of T lymphocytes, leading to the lymphopenia associated with dietary zinc deficiency and malnutrition. Our objective was to examine p56(lck) protein levels, flow cytometric markers of T cell development (CD4, CD8, TCRalphabeta, TCRgammadelta and CD90) and absolute cell numbers in thymus, spleen and blood of zinc-deficient (ZD), diet-restricted (DR) and control (CTL) rats. Recent thymic emigrant (CD90+) T lymphocytes were also investigated after dietary repletion. P56(lck) protein levels were one- to twofold greater in thymocytes than splenocytes, and ZD rats had more thymocyte p56(lck) protein than CTL rats. In the thymus and blood, the proportions of T lymphocyte subpopulations (CD4-CD8-, CD4+CD8+ and CD4+CD- or CD4-CD8+) were unchanged, except for a higher percentage of TCRalphabeta+CD-CD8+ thymocytes in ZD rats. The 15-29% fewer CD90+ T cells in the blood and spleen of ZD rats were reversed after dietary repletion for 7 and 23 d, respectively. In summary, T-cell numbers were proportional to thymus and spleen weights and unaltered per unit blood volume, despite elevated thymocyte p56(lck) protein in ZD rats. In zinc deficiency, the decreased percentages of CD90+ cells in the blood and spleen could adversely affect the T-cell repertoire.     

Depression of thymus-dependent immunity in wasting protein-energy malnutrition does not depend on an altered ratio of helper (CD4+) to suppressor (CD8+) T cells or on a disproportionately large atrophy of the T-cell relative to the B-cell pool.

We report cell numbers within major subsets of lymphocytes in the spleen, mesenteric nodes, and recirculating pool of weanling mice subjected to protein-energy malnutrition (PEM). PEM and thymus (T)-dependent immunodepression were induced in male C57BL/6J mice by a low-protein (LP) diet fed ad libitum. Recirculating lymphocyte numbers were estimated by enumerating labeled and unlabeled cells after equilibration of a known number of fluorescein isothiocyanate-labeled C57BL/6J donor lymphocytes within well-nourished or LP recipients. Involution of the recirculating lymphocyte pool of the LP group was proportionately less than the lymphoid atrophy of the spleen and mesenteric nodes. The LP protocol exerted no influence on the ratio of helper (CD4+) to suppressor (CD8+) T cells and increased the ratio of T cells to B cells in the secondary lymphoid organs and recirculating pool. These results challenge two established concepts: that T-dependent immunodepression in PEM depends on a reduced CD4(+)-CD8+ ratio and that PEM induces greater involution within the T-cell system than within the B-cell system.     

T-cell activity in HLA-associated autoimmune diseases

BACKGROUND: The existence of T-helper-1 (Th1) and T-helper-2 (Th2) subsets has been implicated in the regulation of several immune responses, and alterations in the Th1/Th2 balance have been involved in autoimmunity. The present study investigates the relative influence of Th1 and Th2 patterns in autoimmune responses in patients with HLA-associated autoimmune diseases. METHODS: This study concerns 849 patients of both sexes, suffering from several autoimmune diseases. Tissue typing for HLA antigens of Class I (A, B, C) and Class II (DR, DQ) was carried out in all patients by conventional serologic methods, comparing results with frequencies detected in a normal population. Many immunological tests were also done. In particular, lymphocyte subsets (CD4+, CD8+, CD3-HLA-DR+, NK cells, sIg + B cells) were detected with monoclonal antibodies by a fluorescent cytometer. The changes in frequencies of T cell subsets were used to calculate the possible incidence of two effector phenotypes (TE-1; TE-2). RESULTS: The results of the immunogenetic analysis confirmed the significant HLA-associations in several diseases. The essential T-cell changes were also exposed, thus defining the incidence of T-cell phenotypes (TE-1 = 56.3%; TE-2 = 34.8%). This finding suggested a major impact of cell-mediated immunity, as compared with that of antibody-mediated immunity. CONCLUSIONS: The anomalies of Th1/Th2 balance can impact autoimmune disease, and in many cases a Th2 response can prevent Th1-mediated autoimmunity, which is the most evident phenomenon in several HLA-associated diseases.     

婴幼儿营养性缺铁性贫血的T—淋巴细胞亚群的实验研究

为进一步了解营养性缺铁性贫血与细胞免疫功能的关系,本文测定了44名营养性缺铁性贫血(IDA)婴幼儿的T—淋巴细胞亚群的阳性细胞百分数,并选择了28名健康幼儿为对照组(C组).结果显示缺铁性贫血组(IDA)的CD_3~+％和CD_4~+％较正常对照组明显降低(P<0.01).且与血红蛋白的水平呈显著的正相关(r_1=0.6427,r_2=0.5974 P<0.005);CD_3~+％在正常对照组和缺铁性贫血组间无显著性差异(P<0.05),缺铁性贫血组的CD_4/CD_3比值较正常对照组明显降低,但与血红蛋白的水平无关.提示营养性缺铁贫血的细胞免疫功能低下与CD_3~+％和CD_4~+％降低有关,也是营养性缺铁性贫血易患各种感染性疾病的重要原因之一.     

乳腺癌患者外周血T细胞亚群与NK、NKT细胞检测的临床意义

目的:检测乳腺癌患者外周血T淋巴细胞亚群及NK、NKT细胞表达率的变化,探讨乳腺癌患者免疫状况与肿瘤分期的关系以及免疫指标变化在治疗前后的临床价值。方法 :采用荧光标记法与多参数流式细胞仪检测40例正常女性和120例乳腺癌患者的总T淋巴细胞(CD3+)、T辅助/T诱导细胞(CD3+CD4+)、T抑制/细胞毒细胞(CD3+CD8+)、自然杀伤细胞(NK)、NK样T细胞(NKT)的表达率,并进行统计学分析。结果:无淋巴结转移组乳腺癌患者治疗前CD3+、CD3+CD4、CD3+CD8+、CD4+/CD8+与对照组比较无统计学意义,NK、NKT显著低于对照组(P<0.05)。淋巴结转移组乳腺癌患者CD3+、CD3+CD4、CD3+CD8+、NK、NKT表达率均明显低于对照组(P<0.05)。经过综合治疗后乳腺癌组各项免疫指标均恢复到正常值。结论:外周血T细胞亚群的检测对判断乳腺癌患者的病情、临床分期有一定意义;经过治疗后能够有效地改善乳腺癌患者的免疫状态。     

甲状腺乳头状癌肿瘤微环境中T淋巴细胞各亚群的分布

目的分析甲状腺乳头状癌（PTC）肿瘤微环境T淋巴细胞各亚群的相对分布。方法应用流式细胞术测定35例PTC患者、25例良性甲状腺病变的局部肿瘤组织以及15例健康体检者外周血CD3^＋ CD4^＋ CD8^-、CD3^＋ CD4^- CD8^＋、CD3^＋CD4^＋CD8^＋、CD3^＋CD4^-CD8^-各T细胞亚群的水平及CD4^＋／CD8^＋比值。结果外周血、良性组和PTC肿瘤微环境的CD4^＋／CD8^＋比值明显依次递减（P〈0．01）；PTC和良性组局部组织间的CD3^＋CD4^＋CD8^＋细胞水平显著高于外周血（P〈0．01），但前二者间的CD3^＋CD4^＋CD8^＋细胞水平差异无统计学意义（P〉0．05）；良性组的CD3^＋CD4^-CD8^-细胞水平显著高于PTC局部和外周血（P〈0．01），后二者间的CD3^＋CD4^-CD8^-细胞水平的差异无统计学意义（P〉0.05）；15例伴淋巴结转移PTC局部癌组织的CD4^＋／CD8^＋比值及CD3^＋CD4^＋CD8^-、CD3^＋CD4^-CD8^＋、CD3^＋CD4^＋CD8^＋、CD3^＋CD4^-CD8^-各T细胞亚群的水平与20例无淋巴结转移的PTC局部癌组织之间的差异无统计学意义（P〉0．05）。结论PTC肿瘤微环境免疫细胞以CD8^＋阳性T细胞为主，局部呈明显的免疫受抑状态，而甲状腺良性病变局部CD3^＋CD4^＋CD8^＋、CD3^＋CD4^-CD8^-T细胞的显著增多可能有利于营造抗瘤免疫效应的微环境，PTC肿瘤微环境中T淋巴细胞各亚群的分布与淋巴结癌转移与否无关。