外周血miR-29b、miR-139水平与Th1/Th2的相关性及对银屑病的诊断价值

目的 探讨外周血微小RNA(miR)-29b、miR-139水平与外周血辅助性T淋巴细胞(Th)1/Th2的相关性及对银屑病的诊断价值。方法 选取2020年4月至2021年4月在该院入院治疗的89例银屑病患者为银屑病组,根据银屑病疾病分期将患者分为活动期37例和静止期52例,根据皮损严重程度分为轻度组24例、中度组36例和重度组29例。另选取同期来该院进行体检的体检健康者60例作为对照组。采用实时荧光定量PCR检测外周血miR-29b、miR-139及T细胞转录因子(T-bet)、GATA结合蛋白3(GATA3)水平;流式细胞仪检测外周血Th1、Th2细胞比例;酶联免疫吸附试验检测血清Th1、Th2相关细胞因子水平;Pearson相关分析银屑病患者外周血miR-29b、miR-139水平与Th1/Th2、T-bet、GATA3的相关性;受试者工作特征(ROC)曲线分析外周血miR-139、miR-29b水平对重度银屑病的诊断价值。结果 银屑病组miR-139、T-bet、IL-2、IFN-γ水平、Th1细胞比例、Th1/Th2均高于对照组(P<0.05),miR-29b、GATA...     

寻常型白癜风与晕痣患者外周血T淋巴细胞亚群及Th1/Th2、Th17/Treg细胞平衡比较

目的比较寻常型白癜风与晕痣患者外周血辅助性T细胞1(Th1)、Th2、Th17、调节性T淋巴细胞(Treg细胞)水平,以及Th1与Th2比值(Th1/Th2)、Th17与Treg比值(Th17/Treg)。方法选取43例进展期寻常型白癜风患者和37例Ⅰ、Ⅱ阶段晕痣患者进行研究,均采集外周血进行流式细胞学检测。对照组(n=15)为本院常规体检健康人群。结果寻常型白癜风组病灶多部位者比率显著高于晕痣组多部位者比率(P 0.05)。寻常型白癜风组、晕痣组及对照组Th2、Th17细胞水平及Th17/Treg有显著差异(P 0.05),Th1、Treg细胞及Th1/Th2无显著差异(P 0.05)。结论寻常型白癜风与晕痣患者外周血Th1、Th2、Th17及Treg细胞变化存在差异,表现为寻常型白癜风患者外周血Th17细胞增高更为显著,而晕痣患者外周血Th2细胞下降更显著。     

乳腺癌患者外周血Th1/Th2与雌激素水平相关性研究

目的:探讨乳腺癌患者外周血Th1/Th2平衡状态及血清雌激素水平对其影响。方法:采用流式细胞术检测35例乳腺癌患者和30例健康体检者外周血Th1、Th2及其比值,电化学发光法检测血清雌二醇(E2)水平。结果:乳腺癌患者外周血Th2及血清E2分别为(1.86±0.82)%、(429.46±285.57)pmol/L,高于健康体检组(1.50±0.60)%、(282.41±252.42)pmol/L;Th1及Th1/Th2比值分别为(15.93±5.36)%、10.68±6.92,低于健康体检组(18.68±5.08)%、13.90±5.09,结果均具统计学意义(P<0.05)。乳腺癌患者外周血Th2与血清E2呈正相关(r=0.819,P=0.000)。结论:乳腺癌患者存在高E2水平及Th2优势状态,雌激素对乳腺癌患者Th1/Th2平衡可能起重要作用。     

去白细胞输血对急性淋巴细胞白血病患者外周血Th1/Th2细胞平衡影响

目的:探讨去白细胞输血对急性淋巴白血病(ALL)患者外周血Th1/Th2细胞平衡的影响。方法:选取2016年3月至2017年8月在我院治疗的57例ALL患者,其中31例ALL患者接受常规输血治疗,纳入A组,26例患者接受去白细胞输血治疗,纳入B组,并选择同期在本院体检正常者36例为对照组;记录患者的基本临床特征,采用流式细胞术分析患者外周血Th1/Th2细胞比例;采用ELISA分析患者血清IL-2、IFN-γ、IL-4、IL-10水平;采用RT-PCR分析淋巴细胞T-bet和GATA-3的mRNA表达水平;采用Western blot分析淋巴细胞T-bet和GATA-3蛋白水平。结果:ALL患者外周血Th1/Th2比例与患者的年龄、风险等级明显相关(P0.05);A组患者在接受治疗后Th1/Th2比例变化与治疗前相比无统计学差异(P0.05);B组患者在接受治疗后Th1/Th2比例升高(P0.05)。A组ALL患者Th1和Th2细胞分泌的IL-2、IFN-γ、IL-4和IL-10水平变化无统计学差异(P0.05);B组ALL患者Th1细胞分泌的IL-2和IFN-γ水平均上升,Th2细胞分泌的IL-4和IL-10水平均下降,差异有统计学意义(P0.05)。通过RT-PCR和Western blot检测发现,A组ALL患者T-bet mRNA和T-bet蛋白水平低于对照组,B组ALL患者T-bet mRNA和T-bet蛋白水平高于A组,差异有统计学意义(P0.05)。A组ALL患者GATA-3 mRNA和GATA-3蛋白水平高于对照组,B组ALL患者GATA-3 mRNA和GATA-3蛋白水平低于A组,差异有统计学意义(P0.05)。结论:去白细胞输血有助于改善外周血Th1/Th2细胞平衡,提高患者的免疫功能,这可能与T-bet和GATA-3表达密切相关。     

IgA肾病患者外周血Th1、Th2、Th3细胞水平的变化及意义

目的观察IgA肾病（IgA nephropathy,IgAN）患者外周血中Th1、Th2、Th3细胞亚群的表达,探讨其在IgAN免疫发病机制中的作用。方法采用流式细胞仪检测23例IgAN患者（IgAN组）及20例健康体检者（正常对照组）外周血Th1、Th2、Th3细胞比例,并用Spearman或Pearson相关分析法对其分布变化与IgAN各项临床指标进行相关性分析。结果 IgAN组外周血中Th1细胞比例较正常对照组减少,差异无统计学意义（P〉0.05）;IgAN组外周血中Th2、Th3细胞比例均显著高于正...     

慢性乙型肝炎及乙型肝炎后肝硬化、肝癌患者外周血辅助性T淋巴细胞表达水平分析

目的探讨慢性乙型肝炎(chronic hepatitis B,CHB)及乙型肝炎后肝硬化(liver cirrhosis,LC)、肝癌(hepatocellular carcinoma,HCC)患者外周血辅助性T淋巴细胞(Th)的表达水平。方法选择2014年1—3月建平县疾病预防控制中心传染病网络报告的HBV 34例(CHB组)及2014年1—12月在建平县疾病预防控制中心登记注册的乙型肝炎后LC 31例(乙型肝炎后LC组)和乙型肝炎后HCC 29例(乙型肝炎后HCC组),另选择2014年1—3月参加建平县医院体格检查结论健康的志愿者30例(对照组)。所有入选患者均进行Th(CD3+、CD4+、CD8+、CD4+/CD8+)检测并比较。结果 4组外周血Th各指标值总体比较差异均有统计学意义(P0.05)。CHB组、乙型肝炎后LC组及乙型肝炎后HCC组外周血CD3+、CD4+及CD4+/CD8+值均明显低于对照组,外周血CD8+值均明显高于对照组;乙型肝炎后HCC组外周血CD3+、CD4+及CD4+/CD8+值均明显低于CHB组及乙型肝炎后LC组,而CD8+值明显高于CHB组及乙型肝炎后LC组;乙型肝炎后LC组外周血CD3+、CD4+及CD4+/CD8+值均明显低于CHB组,而CD8+值明显高于CHB组,差异均具有统计学意义(P0.05)。结论 CHB及乙型肝炎后LC、HCC患者均存在明确外周血Th异常表达,乙型肝炎后HCC患者最为严重。     

检测不同活动期强直性脊柱炎患者Th1、Th2、Th17细胞的临床意义

目的初步探讨辅助性T细胞1(Th1)、辅助性T细胞2(Th2)和辅助性T细胞17(Th17)在强直性脊柱炎(AS)发生、发展中的作用及临床意义。方法应用流式细胞术(FCM)及细胞内因子染色技术(ICS)检测78例AS患者(其中低活动性AS 44例、高活动性AS 34例)和30例健康体检者(正常对照组)外周血中Th1细胞、Th2细胞和Th17细胞数量,分析与AS活动性的相关性。结果 AS组、低活动性AS组和高活动性AS组外周血Th1细胞比例分别为15.86%±3.30%、14.18%±2.35%和17.75%±3.14%,均明显高于正常对照组(12.05%±2.35%,P0.01),且高活动性AS组明显高于低活动性AS组(P0.01)。AS组和高活动性AS组外周血Th2细胞比例分别为1.51%±0.51%、1.31%±0.41%,均明显低于低活动性AS组(1.90%±0.51%)和正常对照组(1.98%±0.60%)(P均0.05)。AS组、低活动性AS组和高活动性AS组外周血Th17细胞比例分别为1.57%±0.78%、1.26%±0.57%和2.00%±0.86%,均明显高于正常对照组(0.82%±0.35%,P0.05),且低活动性AS组明显低于高活动性AS组(P0.01)。相关分析显示AS患者外周血Th1细胞、Th17细胞比例与Bath AS病情活动指数(BASDAI)呈正相关[相关系数(r)分别为0.809、0.409,P均0.01],Th2细胞与BASDAI呈负相关(r=-0.340,P0.01)。结论 AS患者CD4+T淋巴细胞亚群功能异常,由此导致的细胞因子网络失衡及体内免疫功能紊乱参与了AS的发生、发展。检测外周血Th1、Th2、Th17细胞数量有助于判断AS病情、提示预后。     

Th1及Th2型细胞的变化与子宫内膜异位症相关性研究

目的 从免疫学角度探讨Th1及Th2型细胞的变化与子宫内膜异位症(EM)的关系.方法 应用流式细胞技术检测40例 EM 及40 例对照组外周血中Th1/Th2型细胞的百分比.结果 EM 组外周血中,Th1型细胞较对照组显著降低(P<0.05);而Th2型细胞较对照组显著升高(P<0.05);EM组Ⅲ～Ⅳ期患者外周血中,...     

乙型肝炎病毒相关慢加亚急性肝衰竭患者Th17及Th1表达意义

目的探讨Th17及Th1在乙型肝炎病毒（hepatitisBvirus,HBV）相关慢加亚急性肝衰竭（sub—acuteandchronicliverfailure,SACLF）患者表达水平及对疾病判断的临床意义。方法HBV相关SACLF患者36例（SACLF组）,慢性乙型病毒性肝炎患者29例（CHB组）,同期体检健康者16例（对照组）,采用流式细胞术检测3组外周血Th17与Th1水平,分析其与肝功能、凝血酶原时间、HBV标志物的相关性;分析Th17、Th1对SACLF发生的预测价值。结果SACLF组与CHB组Th17、Th1与白蛋白、胆碱酯酶呈负相关,与总胆红素、凝血酶原时间呈正相关（P〈0．05）;Th17、Th1、Th1／Th17与HBsAg、HBeAg、HBVDNA均无相关性（P〉0．05）,HBV不同复制状态下Th17、Th1与Th1／Th17水平差异无统计学意义（P〉0．05）;SACLF组Th17、Th1水平高于CHB组与对照组,CHB组高于对照组（P（0．05）,3组间Th1／Th17差异无统计学意义（P〉0．05）;Th17、Th1均能预测SACLF发生,二者联合预测SACLF发生准确性为86．2％,模型方程拟合优度高。结论Th17、Th1与肝脏损伤程度相关,可作为预测SACLF发生及疾病严重程度的判断指标。     

免疫性血小板减少患者外周血Treg及Th1,Th2细胞检测及其临床意义

目的 研究免疫性血小板减少症(primary immune thrombocytopenia,ITP)患者外周血中CD4+CD25+FoxP3+细胞(Treg细胞)及Th1/Th2细胞水平并探讨其临床意义。方法 选取27例临床确诊ITP患者作为实验组,采用流式细胞术检测患者外周血Treg细胞及Th1/Th2细胞的比例,并与25例健康志愿者检测结果进行比较。结果 ITP患者外周血中Treg细胞比例为(2.20±0.93)%,健康对照组为(2.99±0.45)%,ITP患者组Treg细胞低于健康对照组(t=3.820,P<0.01); ITP患者组和健康对照组Th1/Th2细胞水平差异无统计学意义(t_Th1=0.655,t_Th2=0.467,均P>0.05)。结论 ITP患者外周血Treg细胞减少,其对ITP疾病发生发展的重要作用值得深入研究。     

Th1/Th2 imbalance in HCV-related liver cirrhosis

The mechanism by which Hepatitis C virus(HCV) infection promotes the development of hepatocellular carcinoma(HCC) is not known exactly. HCV related HCC occurs frequency in the patients with cirrhosis. There have been reports indicating that Th2-type cytokines down-regulated antitumor immunity, and the activation of type 1 T cell responses produced antitumor immunity. We thought Th1/Th2 imbalance in HCV-related liver cirrhosis might be closely related to the development of HCC. In this study, therefore, we investigated the Th1/Th2 balance at the single lymphocyte level of the patients with HCV-related liver cirrhosis and compared with normal controls by using flow cytometry. Th1-type cytokines(IFN-gamma, IL-2) production was significantly decreased in patients with cirrhosis, whereas Th2-type cytokine production(IL-10) was increased. These suggest Th1/Th2 imbalance in HCV-related cirrhosis would decrease the antitumor immunity and its improvement might present the protective effect from HCC.     

肝细胞肝癌病人Th1/Th2免疫评估

目的 通过检测135例原发性肝癌（PLC）患者手术前后血清IL-2、IL-4、IL-10、IFN-γ、肿瘤坏死因子（TNF）-α水平,并与30例健康查体者（对照组）进行对照分析,观察肝细胞癌病人免疫功能改变并进一步结合临床病理学指标,分析免疫功能变化对临床诊断的指导意义.方法 肝癌病人在术前和术后10d、20d、30d抽取清晨空腹静脉血,对照组在体检合格后抽取空腹静脉血,ELISA技术检测血清IL-2、IL-4、IL-10、IFN-γ、肿瘤坏死因子（TNF）-α浓度.结果 肝癌患者IL-2含量平均为98.2pg/ml,较对照组明显降低（p〈0.05）;IL-4、IL-10含量肝癌组为114.8±27.3 pg/ml和65.6 pg/ml,均明显高于正常对照组（p〈0.01）; TNF-α、IFN-γ血浆含量在肝癌组分别为153.3 pg/ml和89.0 pg/ml,与正常对照组比较均具有显著差异（P〈0.01）.IL-2、TNF-α血清含量在Ⅱ、Ⅲ期肝癌患者均低于Ⅰ期（p〈0.01）,IL-4、IL-10 血浆含量随着病情进展逐渐升高,呈现III期〉II期〉I期趋势,其差异具有显著意义;PHCⅡ、Ⅲ期患者IFN-r含量与Ⅰ期患者间比较差异有显著性.手术后 IL-2、IFN-γ、TNF-α呈逐渐升高趋势,术后20天起血浆浓度与术前血浆浓度相比即具有明显差异（p〈0.05）,术后30天即基本接近正常水平,与术前、术后10天相比具有显著统计学意义（p〈0.05）;IL-4、IL-10呈现逐渐降低趋势,术后20天、术后30天与术前相比其差异具有显著统计学意义（p〈0.05,p〈0.01）.结论 肝癌病人免疫功能失衡,出现Th1/Th2漂移,表现为IL-2、IL-4、TNF-α、IFN-γ、IL-4、IL-10等指标明显改变,并且与肿瘤的分期有关,可作为判断肿瘤分期指标之一.手术切除肿瘤在一定程度上可促进患者免疫失衡的恢复,但手术、麻醉等大的损伤可进一步加重病人的免疫失衡,故不能完全切除病灶者,手术适应症的选择需慎重.     

Innate immune response in Th1- and Th2-dominant mouse strains

C57BL/6 and BALB/c mice are prototypical Th1- and Th2-type mouse strains, respectively. In the present study, we attempted to characterize the innate immune response of macrophages from these mouse strains. Macrophages from C57BL/6 mice produced higher levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin (IL)-12 than those from BALB/c mice after stimulation with macrophage-activating lipopeptide-2 (MALP-2, a synthetic TLR-2 ligand) or lipopolysaccharide (LPS, a TLR-4 ligand). The augmented IL-12 production by C57BL/6 macrophages increased interferon-gamma and, in contrast, decreased IL-13 production by CD4+ T cells. On stimulation with MALP-2 or LPS, C57BL/6 macrophages produced lysosomal enzyme and nitric oxide, effector molecules for bacterial killing, whereas BALB/c macrophages did not. Bactericidal activity of BALB/c macrophages was impaired relative to C57BL/6 macrophages when cells were infected with live bacteria in vitro. In a murine model of septic peritonitis induced by cecal ligation and puncture (CLP), BALB/c mice failed to facilitate bacterial clearance relative to C57BL/6 mice despite an augmented peritoneal leukocyte infiltration that was associated with increased peritoneal levels of cytokines/chemokines. BALB/c mice exhibited increased plasma and hepatic levels of cytokines/chemokines, resulting in an exaggerated systemic inflammation as determined by acute-phase proteins. Finally, BALB/c mice were vulnerable to CLP-induced lethality relative to C57BL/6 mice. Altogether, innate immune response of macrophages is different between these mouse strains, which may affect the development of Th1 and Th2 adaptive immunity in these strains. Reduced systemic inflammatory response in C57BL/6 mice that may result from an eminent local response appears to be beneficial during sepsis.     

Th1/Th2细胞功能变化在婴幼儿毛细支气管炎中的意义

目的：通过观察婴幼儿毛细支气管炎中T辅助细胞Th1/Th2细胞的功能变化，探讨其在毛细支气管炎中的意义。方法：采用ELISA方法测定毛细支气管炎、非喘息性肺炎患儿外周血中干扰素γ（IFN-γ）、白介素4（IL-4）、血清IgE的浓度。结果：（1）毛细支气管炎患儿IL-4的水平显著高于非喘息性肺炎患儿（M=1.66pg&#183;mL^-1，p5=0.05pg&#183;mL^-1，p95=35.46pg&#183;mL^-1）/（M=0.55pg&#183;mL^-1，p5=0.10，p95=21.71pg&#183;mL^-1）（P〈0.05）。（2）IFN-γ在毛细支气管炎中的水平低于非喘息性肺炎，（M=5.93pg&#183;mL^-1，p5=0.59pg&#183;mL^-1，p95=278.93pg&#183;mL^-1）/（M=18.17pg&#183;mL^-1，p5=0.08pg&#183;mL^-1，p95=495.4pg&#183;mL^-1），差异有统计学意义（P〈0.05）。（3）毛细支气管炎患儿IgE的浓度略高于非喘息性肺炎，（M=71.24，p5=2.35pg&#183;mL^-1，p95=867.13IU&#183;mL^-1）/M=19.58，p5=3.68pg&#183;mL^-1，p95=211.90IU&#183;mL^-1），但差异无统计学意义（P〉0.05）。结论：婴幼儿毛细支气管炎中可能存在Th2优势反应。     

Th1 and Th2 subsets in orthopaedic surgery by single-cell cytokine analysis

Significant immunosuppression can occur following allogeneic blood transfusion or surgery. Cytokine stimulation controls immune responses and determines their type and intensity. Infusion of autologous or allogeneic blood provides elements, including cytokines, which may result in transfusion-associated immunomodulation. This study investigates to what extent autologous/cell salvage transfusions affect levels of intracellular cytokines interferon-gamma and interleukin-4, and if this indicates a shift in the T-helper 1/T-helper 2 cell ratio using a novel method of detecting intracellular cytokines, the Magnetic Activated Cell Sorter Cytokine Secretion Assay (MACS Assay). Comparisons were made between patients receiving autologous blood or no blood transfusion, for pre- and post-operation levels of interferon-gamma and interleukin-4. Interferon-gamma producing T-helper 1 cells decreased post-operatively. Concomitantly, interleukin-4 producing T-helper 2 cells increase. These results demonstrate a measurable shift from T-helper 1 to T-helper 2 cells post-operatively. Secondly, the study showed surgery alone instigates the same level of immunomodulation as autologous/cell salvage blood transfusion in combination with surgery.     

Th1/Th2 cells in inflammatory disease states: therapeutic implications

Inflammation is initiated as a protective response by the host, but can often result in systemic pathology. Among cells of the immune system, T lympho-cytes play a major role in the inflammatory response. T cell inflammation is characterised histologically by an infiltration of mononuclear cells. Key regulators of this response are a subset of T lymphocytes called T helper (Th) cells. These cells secrete soluble mediators called cytokines, which orchestrate the immune response. The appropriate regulation of Th cell immunity is critical in the control and prevention of diverse disease states. This review will focus on the role of Th cells in the inflammatory process involved in allergic disease, diabetes, infectious disease, rheumatoid arthritis, heart disease, multiple sclerosis and cancer. In the area of autoimmunity, in particular, a basic understanding of Th cells and cytokines has contributed to the development of clinically efficacious biological agents. This review also examines current and novel treatment strategies under investigation at present that regulate Th cell immunity, which may result in better treatments for immune-mediated diseases.     

Th1/Th2细胞因子在强直性脊柱炎中的表达及其意义

目的 探讨Th1/Th2细胞因子网络在强直性脊柱炎(As)中的变化及其意义.方法 采用酶联免疫吸附试验(ELISA)法测定AS患者血清和关节液Th1/Th2型细胞因子TNF-α、IL-17、IL-10和IL-4的水平,并与正常对照组比较.结果 AS患者血清TNF-α、IL-17和IL-10水平均显著高于正常对照组,IL-4的水平显著低于正常对照组;AS患者关节液TNF-α、IL-17和IL-4的水平显著高于血清水平.结论 AS患者血清Th1/Th2型细胞因子表达存在异常,为Th1优势型.     

Th17与1型糖尿病

1型糖尿病是一种以选择性易感人群胰岛β细胞损害、胰岛素分泌绝对减少为特征的自身免疫性疾病。其发病率在全球持续增长,每300人当中就有1人患有1型糖尿病。1型糖尿病的病因和发病机制较复杂,至今仍未完全明确,认为其与自身免疫紊乱密切相关,近年来有研究认为其发病与Th17密切相关,本文将Th17的相关特征及其介导1型糖尿病发病的机制进行综述。