米诺环素局部用药对牙周炎患者龈沟液中超氧化物歧化酶活性的影响

目的： 比较应用米诺环素软膏治疗前后牙周炎患者临床指标及龈沟液中超氧化物歧化酶(SOD)活性的变化。方法：成人牙周炎患者30例，分成2组，实验组常规牙周治疗+米诺环素软膏，对照组仅给予常规牙周治疗，记录两组治疗前后牙龈指数（GI）、牙周探诊深度（PD）、附着丧失(AL)； 并用放射免疫法检测两组龈沟液SOD活性。结果：（1）治疗后，试验组牙龈指数（GI）、牙周探诊深度（PD）均明显低于对照组（P<0.05，P<0.01）；而治疗前两组比较，上述指标未见显著差别。 （2）治疗前，实验组龈沟液SOD活性与对照组比较无显著差别（P>0.05），而治疗后，实验组龈沟液SOD活性显著高于对照组 (P<0.05)。结论：米诺环素局部用药可显著降低牙周炎患者牙龈指数（GI）、牙周探诊深度（PD）并提高龈沟液SOD活性。     

盐酸米诺环素缓释抗菌软膏修复牙周牙髓联合病变

背景:关于盐酸米诺环素对慢性牙周炎、牙周脓肿等的辅助治疗作用已有较多报道,但有关盐酸米诺环素在牙周牙髓联合病变中的详细研究,目前国内报道较少。目的:探讨缓释型盐酸米诺环素在牙周牙髓联合病变临床治疗中应用的效果。方法:选择68例(68颗)牙周牙髓联合病变患者进行牙周机械治疗联合根管治疗,按患者意愿分3组进行牙周治疗,实验组(n=24)牙周机械治疗后局部注入盐酸米诺环素,阳性对照组(n=24)牙周机械治疗后局部注入碘甘油,阴性对照组(n=20)牙周机械治疗后未注入药物,3组均于牙周机械治疗后1个月复诊,比较治疗前后牙周探诊深度、附着丧失和龈沟出血指数的差异。结果与结论:牙周机械治疗1个月后,3组牙周探诊深度、附着丧失和牙龈出血指数均较治疗前明显改善(P0.05);实验组与阳性对照组牙周探诊深度和龈沟出血指数优于阴性对照组(P0.05),实验组牙周探诊深度和龈沟出血指数优于阳性对照组(P0.05)。表明在牙周牙髓联合病变的临床治疗中,除了采取牙周机械治疗和根管治疗,局部应用缓释型盐酸米诺环素能有效降低牙周袋深度,改善牙龈炎症状况,巩固临床治疗效果。     

盐酸米诺环素软膏治疗中重度慢性牙周炎的临床疗效

目的 探讨盐酸米诺环素软膏治疗中重度慢性牙周炎的临床疗效。方法 选取2017年2月~2019年3月我院口腔科收治的中重度慢性牙周炎患者136例,随机分为观察组和对照组,各68例。对照组予碘甘油治疗,观察组以盐酸米诺环素软膏治疗。比较两组出血指数（BI）、牙龈指数（GI）、探诊深度（PD）、牙齿松动度（TM）及临床效果。结果 观察组PD、BI、GI、TM分别为（2.31±0.21）mm、（0.78±0.23）分、（0.66±0.18）分、（0.17±0.08）mm,均低于对照组的（3.20±0.43）mm、（1.22±0.36）分、（1.37±0.37）分、（0.30±0.15）mm,差异有统计学意义（P＜0.05）;观察组治疗总有效率高于对照组,差异有统计学意义（P＜0.05）。结论 对中重度慢性牙周炎患者局部予以盐酸米诺环素软膏疗效显著,可有效降低患者牙龈出血及牙齿松动情况。     

慢性牙周炎治疗前后患牙龈沟液中IL-8和TNF-α水平变化比较

目的:探讨慢性牙周炎患者治疗前后患牙龈沟液中IL-8和TNF-α水平的变化。方法:选择本院就诊的慢性牙周炎患者96例为研究组和健康体检的牙周健康者50例作为对照组,研究组在基础治疗前后收集龈沟液(GCF)并记录相关的临床指标,包括探诊深度(PD)、临床附着丧失(CAL)和探诊出血点数(BOP),应用ELISA法测定两组龈沟液内IL-8和TNF-α的含量。结果:研究组治疗前牙周临床指标PLI、PD、SBI和AL均明显高于对照组,经过牙周基础治疗后,各指标均较治疗前显著下降(P0.05)。研究组治疗前和治疗后龈沟液IL-8和TNF-α水平均显著高于对照组(P0.05)。研究组治疗后龈沟液IL-8和TNF-α水平均明显较治疗前降低(P0.05)。研究组治疗前龈沟液中IL-8的水平与PD呈显著正相关(r=0.486,P0.05),TNF-α水平与SBI、PD呈显著正相关(r=0.495,0.648,P0.05)。结论:慢性牙周炎疾病发生发展与龈沟液中IL-8和TNF-α浓度之间存在着密切联系,且反映了慢性牙周炎的严重程度。     

牙周基础治疗对糖尿病患者血清和龈沟液中肿瘤坏死因子-α的影响

目的 观察牙周基础治疗对2型糖尿病伴牙周炎患者血清及龈沟液中肿瘤坏死因子-α(TNF-α)的浓度、临床牙周状态、血糖控制的影响.方法 选取2型糖尿病伴牙周炎患者60例,随机分做牙周基础治疗组(观察组)和不做牙周基础治疗组(对照组),每组各30人.分别在治疗前、治疗后1个月和3个月记录所有患者牙周临床指数:探诊深度(PD),附着丧失(AL)及菌斑指数(PLI),并检测糖化血红蛋白(HbAlc)及血清及龈沟液中TNF-α的含量.结果 观察组中PD、PLI和龈沟液中TNF-α含量在治疗后1个月和3个月时均显著降低(P＜0.05),AL和血清中HbAlc及TNF-α含量仅在治疗后3个月显著降低(P＜0.05).对照组的PD,AL,PLI,HbAlc,龈沟液及血清中TNF-α的差异无统计学意义(P＞0.05).结论 牙周基础治疗有助于2型糖尿病伴牙周炎患者的血糖控制,牙周状态改变和血清及龈沟液中TNF-α含量下降.     

慢性牙周炎经口腔正畸辅以牙周组织再生术治疗效果及牙周指数分析

目的:分析口腔正畸联合牙周组织再生术治疗慢性牙周炎的临床疗效.方法:选取2019年11月至2021年12月本院收治的慢性牙周炎患者95例,根据治疗方式不同分为对照组(口腔正畸)41例,观察组(口腔正畸联合牙周组织再生术)54例.治疗后3周对比两组临床疗效、牙周指标[牙龈指数(Gingival index,GI)、菌斑指数(Plaque index,PLI)、龈沟出血指数(Gingival sulcus bleeding index,SBI)、牙周深度(Periodontal depth,PD)]及疼痛情况.结果:观察组总有效率为96.29％高于对照组82.93％(P＜0.05);观察组GI、PLI、SBI、PD明显低于对照组(P＜0.05);治疗前两组VAS评分比较无差异,治疗后两组VAS评分均降低,而观察组VAS评分明显低于对照组(P＜0.05).结论:口腔正畸联合牙周组织再生术治疗慢性牙周炎的临床疗效好.     

螺旋霉素联合替硝唑局部给药治疗慢性牙周炎的临床疗效观察

目的：观察螺旋霉素联合替硝唑治疗慢性牙周炎的临床疗效。方法将我院收治的82例慢性牙周炎患者按照随机对照原则分为螺旋霉素联合替硝唑治疗组(观察组)和对照组单纯替硝唑治疗组(对照组),每组各41例,比较两组患者治疗后的牙龈指数(GI)、菌斑指数(PLI)、龈沟出血指数(SBI)以及牙周袋探诊深度。结果观察组患者治疗后的GI、PLI、SBI以及牙周袋探诊深度均较对照组患者有显著改善,组间差异有统计学意义(P<0.05)。结论螺旋霉素联合替硝唑治疗慢性牙周炎疗效确切,值得临床进一步推广使用。     

医用透明质酸修复慢性牙周炎:可有效促进牙周组织再生

背景:据报道,透明质酸具有抗氧化、调节免疫、抗炎和促进伤口愈合等作用。在口腔治疗领域,越来越多的临床医师将透明质酸应用于慢性牙周炎治疗取得了显著疗效。目的:观察局部应用医用透明质酸钠治疗慢性牙周炎的临床效果。方法:选择60例慢性牙周炎患者共300颗患牙,采用口内自身对照的研究方法,受试者A区前磨牙、磨牙150颗为实验组,受试者B区前磨牙、磨牙150颗为对照组。实验组治疗方法为牙周洁治、龈下刮治治疗与龈沟内局部应用Gengigel凝胶(0.2%透明质酸)涂抹上药,对照组进行与实验组相同的全部牙周基础治疗,之后不进行龈沟内上药处理。随访8周,观察两组患牙菌斑指数、龈沟出血指数、牙周探诊深和附着丧失水平等临床指标的动态变化。结果与结论:两组临床治疗后各项检测指标较治疗之前都有明显改善。两组治疗1,4,8周的菌斑指数比较差异无显著性意义;实验组治疗后1,4,8周的龈沟出血指数低于对照组(P0.05);实验组治疗4,8周后的牙周探诊深度均低于对照组(P0.05);实验组治疗8周后的附着丧失低于对照组(P0.05)。结果表明,牙周基础治疗配合龈沟内局部应用医用透明质酸钠治疗慢性牙周炎临床疗效显著。     

盐酸米诺环素软膏与甲硝唑对种植体周围炎抑制作用的 临床研究

目的 对比局部运用盐酸米诺环素软膏和口服甲硝唑治疗种植体周围炎的临床效果。方法 选取2016年3月~2018年5月来我院口腔门诊就诊的种植体周围炎患者36例,在对种植体进行龈上洁治、龈下刮治后,随机分为盐酸米诺环素软膏组和口服甲硝唑组,每组18例。盐酸米诺环素软膏组局部运用盐酸米诺环素软膏治疗,口服甲硝唑组采用口服甲硝唑治疗。对比基线、治疗后4周和8周改良菌斑指数（mPLI）、改良龈沟出血指数（mSBI）和探诊深度（PD）参数。结果 治疗后4周及8周,两组患者mPLI、mSBI以及PD参数均低于基线,差异具有统计学意义（P＜0.05）;盐酸米诺环素软膏组治疗后4周及8周的mPLI、mSBI以及PD参数均低于口服甲硝唑组[4周:mPLI（0.69±0.47）vs （1.46±0.33）,mSBI（0.62±0.41） vs （1.16±0.38）,PD（2.47±0.37）mm vs （3.52±0.54）mm;8周:mPLI（0.98±0.39）vs（1.52±0.30）,mSBI（0.86±0.45）vs（1.26±0.43）,PD（2.93±0.50）mm vs （3.46±0.46）mm],差异具有统计学意义（P＜0.05）。结论 盐酸米诺环素软膏和甲硝唑治疗种植体周围炎有确切的疗效,应用盐酸米诺环素软膏效果明显优于口服甲硝唑,局部使用药物可以实现短期的大幅优化。     

结合四环素和抗炎剂的牙周治疗对早发性牙周炎抗牙龈卟啉菌抗体水平的影响

目的：评价结合四环素和抗炎剂的牙周治疗对早发性牙周炎抗牙龈卟啉菌抗体IgG水平的影响。 方法： 研究对象由12名早发性牙周炎患者、16名成年牙周炎患者和12名牙周健康者组成。牙周炎患者接受常规牙周治疗，并口服四环素和消炎痛，牙周治疗前及治疗3个月后常规临床检查、记录牙周探诊深度和牙周附着水平及牙周探诊出血指数，并测定治疗前后患者血清抗牙龈卟啉菌抗体IgG滴度的变化。 结果： 牙周治疗后，牙周探诊深度、附着水平和牙周出血指数均有显著改善，血清抗牙龈卟啉菌抗体滴度明显下降。 结论： 结合四环素和消炎痛的机械性牙周治疗对早发性牙周炎可获得满意的疗效。血清抗牙龈卟啉菌抗体水平对评估早发性牙周炎的预后具有重要意义。     

雷公藤甲素抗肿瘤血管新生的研究进展（英文）

雷公藤甲素(triptolide,TPL)是从中草药雷公藤中提取的一种有效活性物质,已被用来治疗多种疾病,包括系统性红斑狼疮,类风湿性关节炎,肾病综合征等,TPL甚至有很强的抑制肿瘤的活性。近些年的研究显示,TPL具有抗血管新生的能力,TPL不仅可以抑制肿瘤的增殖,诱导细胞的凋亡,还可以抑制肿瘤的转移,可以增加其它化疗药物的抗肿瘤活性。本综述将讨论TPL在抗肿瘤血管新生方面的研究进展,以及初步探讨其潜在的作用机制。     

Changes in activity of neutral proteases in tissues of ground squirrels in the dynamics of hibernation

Total activities of neutral proteases in the cerebral, hepatic, and myocardial tissues of ground squirrel vary during hibernation: in autumn (before hibernation) activities of the enzymes in the brain and myocardium start increasing, while in the liver they do not change. A common feature for all tissues is minimum activity of active neutral proteases in the middle of hibernation month 1 bout, while the maximum activity is recorded before awakening. Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 146, No. 9, pp. 278–280, September, 2008     

Kinetics of decline of maternal measles virus-neutralizing antibodies in sera of infants in France in 2006

The optimal age for measles vaccination is an important health issue, since maternal antibodies may neutralize the vaccine antigen before a specific immune response develops, while delaying vaccination may increase the risk of complicated diseases in infants. However, measles vaccination impacts the duration of protection afforded by transplacental transfer of maternal antibodies: vaccination-induced maternal antibodies disappear faster than disease-induced antibodies. In order to maintain protection against measles in infants, it is important to monitor the dynamics of this phenomenon in vaccinated populations. To assess the current situation in France, a multicenter, prospective seroepidemiological study was conducted in seven French hospitals between October 2005 and January 2007. Maternal measles antibody concentrations from 348 infants 0 to 15 months old were measured using the plaque reduction neutralization assay. Geometric mean concentrations and the percentage of infants with maternal measles antibody concentrations above the protection threshold (≥120 mIU/ml) were assessed according to age. Results show that after more than 20 years of routine measles vaccination in France, maternal measles-neutralizing antibodies decrease dramatically in French infants by 6 months of age, from 1,740 mIU/ml for infants 0 to 1 month old to 223 mIU/ml for infants 5 to 6 months old, and that 90% of infants are not protected against measles after 6 months of age. Infant protection against measles could be optimized both by increasing herd immunity through an increased vaccine coverage and by lowering the age of routine vaccination from 12 to 9 months.     

Effect of vitamin C in reducing the toxicity of endosulfan in liver in rabbits

In this study, the effect of endosulfan, an organochlorine pesticide, and the ameliorating effect of vitamin C on the livers of New Zealand white rabbits were studied. Livers of the rabbits were examined grossly and histopathologically, and caspase-3 activity was detected by immunohistochemical methods. A total of twenty-four rabbits were divided into four groups (n=6). Rabbits in Group I (END) were daily given a sublethal dose of endosulfan (1 mg/kg bw) in corn oil by oral gavage for 6 weeks. Group II (END+C) received the same dose of endosulfan and additionally Vit C (20 mg/kg bw) every other day during this period. Group III (OIL+C) received corn oil daily by oral gavage and vitamin C every other day for 6 weeks. Group IV (OIL), the control group, received only corn oil daily, by oral gavage throughout the experiment. The concentration of α-endosulfan in the END group was higher in livers (0.102±0.012 ppb) than the β-endosulfan (0.072±0.001 ppb). Decreased accumulation of α and β endosulfan was observed in the END+C group (0.025±0.003 and 0.016±0.002 ppb, respectively) (p<0.0001). The most prominent gross findings at the necropsy were seen in the END group, in which swollen and pale livers were commonly observed. Hemorrhages, degenerations, necrosis, and in some rabbits bile duct hyperplasia were the marked histopathological findings of the END group. Caspase-3 positive reaction was more severe in this group than in the others. An ameliorating effect of Vit C on gross, histopathological and immunohistochemical findings was observed in the END+C group. The results revealed that endosulfan is highly toxic for rabbit livers. However, toxicity was decreased by Vit C treatment, which reduced the accumulation of endosulfan in livers four-fold.