妇科炎症患者沙眼衣原体和解脲支原体的检测分析

目的 了解妇科炎症患者沙眼衣原体（CT）及解脲支原体（UU）的感染情况,并研究其相关性和致病机理。方法 运用荧光定量聚合酶链反应（VQ-PCR）技术对820例妇科炎症患者生殖道分泌物进行CT和UU检测并分析。结果 458例阴道炎患者CT和UU的检出率分别为10．26％和65．94％．270例官颈炎患者CT和UU的检出率分别为11．85％和60．00％,92例盆腔炎患者CT和UU的检出率分别为3．26％和45．65％,CT和UU同时阳性阴道炎患者有24例、宫颈炎患者有13例、盆腔炎患者有1例。在各组中CT与UU的阳性率比较有显著差异（P〈0．01）,而各组之间CT、UU的阳性率无显著差异（P〉0．05）。结论 提示CT、UU的感染与妇科炎症密切相关,主要表现为免疫性条件致病,对妇科炎症患者应重视CT和UU的检测。     

4种性病病原体感染16450例流行病学调查报告

目的：调查沙眼衣原体(CT)、解脲脲原体(UU)、淋球菌(NG)、人乳头瘤病毒(HPV)等性病病原体感染的流行病学状况。方法：对江西省12家市级医院性病门诊就诊的16450例泌尿生殖道感染患者留取的分泌物采用荧光定量PCR方法检测CT-DNA、UU-DNA、NG-DNA、HPV-DNA。结果：标本中CT、UU、NG、HPV 12733例男性阳性率分别为16．4％、9．1％、7．1％、6．5％,3717例女性阳性率分别为22．0％、21．1％、10．5％、2．1％;CT UU混合阳性检出率6．3％,CT NG混合阳性检出率为0．6％,CT HPV混合阳性检出率为1．4％,UU NG混合阳性检出率为3．4％,UU HPV混合阳性检出率为3．7％,NG HPV混合阳性检出率为0．7％,两种以上混合感染例数的阳性检出率为1．0％;2002年、2003年阳性检出率分别为37．1％、48．5％。结论：江西省CT、UU、NG、HPV4种性病病原体的流行较为严重,且呈逐年增长的趋势。     

非淋菌性阴道炎感染沙眼衣原体及支原体98例检测分析

目的探讨非淋菌性阴道炎患者与宫颈沙眼衣原体及支原体感染的相关性。方法取宫颈分泌物进行沙眼衣原体（CT）及支原体（UU）检测，CT采用抗原免疫法；UU采用培养法。结果98例中，CT和UU的检出率分别为23．5％和31．4％，两者感染的总阳性率为54．9％，两者混合感染率为9．6％。结论提示沙眼衣原体及支原体感染为非淋菌性阴道炎的重要致病因素，对非淋菌性阴道炎患者应重视进行CT和UU的检测。     

慢性前列腺炎混合感染的研究

目的：对慢性前列腺炎患者淋球菌、沙眼衣原体、解脲支原体感染进行单一病原体及多种病原体混合感染研究。方法：采用聚合酶链反应(PCR)对慢性前列腺炎289例的前列腺分泌物进行淋球菌(NG)、沙眼衣原体(CT)、解脲支原体(UU)检测。结果：289例中NG检出率为10．2％，UU检出率为30．6％，CT检出率为14．2％，NG UU检出率为4．5％，NG UU CT检出率为1．5％，UU CT检出率为8．5％，NG CT栓出率为2．8％。结论：对原因不明的慢性前列腺炎病人，有必要对其感染的病原体进行检测。     

阴道炎患者沙眼衣原体和支原体感染状况及耐药性分析

目的:调查阴道炎患者CT、UU、Mh的感染情况及其对常用药物的耐药率。方法:对1285例患者的宫颈分泌物检测沙眼衣原体(CT)及解脲支原体(UU)、人型支原体(Mh)培养及药敏实验。结果:CT阳性98例(7.63%),UU阳性457例(35.56%),CT合并UU阳性52例(4.05%),UU合并MH阳性93例(7.24%)。UU对10种抗生素耐药性,前四位依次为四环素、氧氟沙星、司帕沙星、左氧氟沙星。结论:CT、UU是引起阴道炎的主要病原之一,支原体的耐药监测对指导临床治疗具有重要意义。     

性传播疾病三种病原体检测结果分析

目的 通过对三年问北京市密云地区淋病奈瑟菌(NG)、沙眼衣原体(CT)、解脲支原体(UU)检测的结果分析，了解三种病原体的感染情况，为临床医生诊断和治疗及流行病学研究提供依据。方法 应用聚合酶链式反应(PCR)技术对2000～2002年550例有性传播疾病(STD)相关临床症状及体征的患者的泌尿生殖道标本进行淋病奈瑟菌(NG)、沙眼衣原体(CT)、解脲支原体(UU)的检测。结果 550份标本中，三种病原体阳性共127份，总检出阳性率23．10％。2000年感染的阳性检出率21．47％，2001年感染的阳性检出率22．34％，2002年感染的阳性检出率27．08％。单一病原体感染108例，2种病原体混合感染19例。在3年中NG的阳性检出率15．82％，CT的阳性检出率6．36％，UU的阳性检出率4．36％。在127份阳性标本中，21～40岁年龄组检出病原体103份，占全部阳性例数的81．10％。结论 性传播疾病(STD)呈逐年上升趋势，主要集中在青壮年人群；以NG单一感染为主，也伴有2种病原体的混合感染，提示泌尿生殖道感染病人应重视对这三种病原体的检出，避免漏检，及时诊断和治疗由这些病原体引起的性病，防止性传播疾病(STD)的扩散。     

广东地区淋球菌、沙眼衣原体及解脲脲原体感染脲的分子行病学调查

目的：了解广东地区淋球菌(NG)、沙眼衣原体(CT)、解脲脲原体(UU)等性病的流行病学状况。方法：对广东地区76家基层医院性病门诊就诊的1949例生殖道感染患者取其分泌物，用PCR方法检测NG，CT，UU—DNA。结果：262例男性患者NG，CT，UU阳性检出率分别为7．63％，23．28％，10．69％；1687例女性患者NG，CT，UU阳性检出率分别为9．19％，17．90％，17．19％。男性CT感染率23．28％明显高于女性17．90％(P＜0．05)，女性UU感染率17．19％高于男性10．69％(P＜0.05)，男、女性NG阳性率分别为7．63％和9．19％差异无显著性(P＞0．05)。NG CT混合感染率为3．27％，NG UU混合感染率为2．34％，CT UU混合感染率为8．88％，NG CT UU混合感染率为0．93％。1999—2001年3种性病感染率分别为26．11％，43．81％和51．52％。结论：广东地区NG，CT，UU 3种性病的流行较为严重、总感染率高达43．92％；反呈递年增长的趋势。     

解脲支原体、衣原体感染与反复自然流产的关系

目的 研究解脲支原体（UU）、衣原体（CT）对反复流产的影响。方法 应用聚合酶链反应（PCR）技术分别对78例反复流产患者（流产组）和89例人工流产孕妇（对照组）的宫颈分泌物进行UU、CT检测。结果 流产组UU的阳性率为34．61％，CT的阳性率为41．03％，与对照组比较，差异有显著性（P〈0．01），UU阳性检出率随流产次数增加而增高，CT与UU复合感染也随着流产次数增加而增高，单纯CT感染与流产次数无明显正相关关系。结论 UU、CT是引起反复自然流产的重要感染源。     

妇科门诊580例阴道炎患者STD感染状况调查

目的:了解妇科门诊性传播疾病 (STD)感染状况。 方法 :应用病原体培养、快速免疫检测及显微镜下直接检测等方法 ,对妇科门诊初诊的 5 80例阴道炎患者 ,取宫颈拭子进行淋球菌 (NG)、支原体 (UU)、衣原体(CT)、念珠菌及滴虫等检测。 结果 :NG阳性检出率为 2 5 .86 % ,UU阳性检出率为 16 .72 % ,CT阳性检出率为 3.97% ,念珠菌阳性检出率为 2 7.0 7% ,滴虫阳性检出率为 9.4 8% ,结论:应加强门诊 STD的筛查和治疗     

湖南省衡阳地区STD患者病原体检测

目的 了解衡阳地区性传播疾病(STD)患者病原体感染情况。方法 对2420例可疑STD患者进行检测：淋病奈瑟菌(NG)、解脲脲原体(UU)、人型支原体(MH)采用培养法；沙眼衣原体(CT)、人乳头瘤病毒(HPV)、单纯疱疹病毒(HSV)核酸采用聚合酶链反应(PCR)法；梅毒螺旋体(TP)采用快速血浆反应素环状卡片试验(RPR)和TP-PA法。结果 STD病原体阳性检出率为38．1％(922／2420)。在922例中UU占29．6％，CT20．8％，HPV17．8％，NG17．4％，MH6．3％，HSV4．2％，TP3．9％。结论 衡阳地区STD患者中UU感染率最高，CT和HPV次之，TP感染率最低。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.