外周血DC-CIK对人舌鳞癌细胞系Tca8113的抑瘤作用

目的:探讨体外致敏成熟树突状细胞(DC)与细胞因子诱导杀伤细胞(CIK)共培养细胞群对人舌鳞癌细胞系Tca8113移植瘤裸鼠的体内抑瘤效果.方法:人舌鳞癌外周血分离培养DC,体外诱导培养CIK细胞,将致敏成熟的DC与CIK体外扩增获得共培养细胞群DC-CIK细胞;于裸鼠单侧腋下接种Tca8113,将实验动物分为对照组A、实验组B和实验组C;接种后24h,A组移植瘤区注射生理盐水,B组于对侧腋下注射DC-CIK,C组同侧腋下注射DC-CIK,饲养4周；观察移植瘤的成瘤时间、成瘤率、生长曲线及组织学观察,采用SAS 6.12软件包对数据进行t检验、方差分析和x2检验.结果:A组平均成瘤时间为7.63d,B组为9.5d,C组为12d,A、C组有显著差异(P=-0.0132);注射后2周,A组成瘤率为100％,B组为87.5％,C组为62.5％,P＞O.05;观察期内,从移植瘤生长曲线观察,A组瘤体增长最快,B组其次,C组最慢,A、B组无显著差异(P＞0.05),A、C组差异显著(P=0.036).结论:致敏DC-CIK共培养细胞对Tca8113移植瘤裸鼠有一定的体内抑瘤效应.     

放射诱导启动子介导双自杀基因治疗舌鳞癌的实验研究

目的探讨放射诱导启动子介导双融合自杀基因CDglyTK靶向治疗人舌鳞癌裸鼠移植瘤的疗效。方法构建人舌鳞癌裸鼠移植瘤模型．以脂质体为载体介导携放射诱导启动子调控双融合自杀基因的真核表达质粒pcDNA3．1（＋）／E-CDglyTK瘤内转染,辅以3Gy放疗,腹腔注射5-氟胞嘧啶和丙氧鸟苷．描绘肿瘤生长曲线,RT—PCR检测CDglyTK的mRNA水平．免疫组化检测细胞核增殖抗原的表达．原位末端标记法检测肿瘤细胞凋亡。结果放射诱导启动子介导的CDglyTK对肿瘤生长有明显抑制作用．RT—PCR可检测到转染后肿瘤细胞CDglyTK的mRNA表达,诱导放疗增强了其表达水平．电泳条带灰度值从0．213±0．023上调至0．279±0．038（P〈0．01）;诱导放疗显著提高疗效,凋亡指数从21．52％上升为32．23％（P〈0．01）,增殖指数由28．36％下降至20．07％fP〈0．01）。结论放射诱导启动子可作为基因治疗分子开关调节CDglyTK基因在人舌鳞癌裸鼠移植瘤中靶向表达．低剂量放射性照射可显著提高其疗效。     

树状细胞疫苗抑制肿瘤的实验观察

目的观察经肿瘤抗原致敏的树状细胞（DC）疫苗对荷瘤裸鼠的抑瘤作用。方法重组人粒细胞-巨噬细胞集落刺激因子（rhGM-CSF）、重组人白细胞介素4（rhIL-4）、重组人肿瘤坏死因子α（rhTNF-α）协同诱导人外周血单核细胞成为DC。Tea8113舌癌细胞可溶性抗原体外致敏DC，将同源人T淋巴细胞与之共同培养诱导出抗原特异性的细胞毒性T淋巴细胞（CTLs），共同接种至荷瘤裸鼠体内行免疫治疗。结果人外周血单核细胞在细胞因子协同诱导下可获得功能正常的DC，用致敏DC＋CTLs免疫治疗的荷瘤裸鼠与其他实验组相比，明显抑制肿瘤生长，肿瘤倍增时间显著提高（P〈0．05）。结论从人外周血来源的单核细胞在多种细胞因子作用下可诱导为功能正常的DC、冻融抗原体外致敏的DC，经其激活的肿瘤特异性CTLs对荷舌鳞癌裸鼠有明显的免疫治疗作用，可望成为口腔颌面部舌癌免疫治疗的新途径。     

TNP—470、顺铂联合应用抗人舌癌生长的实验研究

目的：研究血管生成抑制剂TNP－470对人舌癌生长的抑制作用。方法：MTT法 定TNP－470、顺铂（CDDP）对体外培养人舌癌Tca8113细胞增殖的抑制作用。用Tca8113细胞形成裸鼠肿瘤皮下移植模型,移植二周后腹腔注射用药,TNP－470（30mg/kg)隔日一次,共七次,CDDP（5mg/kg)一周二次,共四次,同期测量肿瘤体积和裸鼠体笪“移植四周后处死动物,检测肿瘤体积和主要脏器。结果：TNP－470作用Tca8113细胞的半数抑制浓度（IC50）为70．79μg/ml。TNP-470 CDDP组抑瘤率分别为31．7％、40．3％和65．6％,实验组与对照组之间抑瘤率有显著性差异（P＜0．05）,TNP－470＋CDDP组抑瘤效果最佳,结论TNP－470对人舌癌体内生长有一定抑制作用,联合应用CDDP可进一步提高治疗效果。     

高迁移率族蛋白N2对人舌癌裸鼠移植瘤的抑瘤作用研究

目的 研究高迁移率族蛋白N2（HMGN2）对人舌癌裸鼠移植瘤生长的抑瘤作用。方法 裸鼠腋下注射接种舌鳞癌细胞 Tca8113以建立裸鼠移植瘤模型,接种 7d成瘤后,设立阴性对照组、 HMGN2蛋白组和阳性对照组,在瘤体周边分别注射含 washingbufferⅡ、 HMGN2蛋白和顺铂的细胞培养基。观察各组裸鼠肿瘤的生长情况,经过4次给药后处死裸鼠,取出瘤块,称其质量计算抑瘤率,并行苏木精-伊红（HE）染色观察形态学变化。结果 成功建立舌癌裸鼠移植瘤模型。HMGN2蛋白组和阳性对照组肿瘤体积明显小于阴性对照组。裸鼠体重在整个实验过程中并没有明显的变化。阴性对照组、HMGN2蛋白组、阳性对照组的肿瘤质量平均值分别为（0.38±0.19）、（ 0.21±0.15）、（0.23±0.16）g,3组间的差异无统计学意义。HMGN2蛋白组和阳性对照组的抑瘤率分别为45.71%和39.44%。肿瘤经肉眼观察可见阴性对照组瘤块较大,HE染色可见 HMGN2蛋白组和阳性对照组细胞坏死明显。结论 HMGN2蛋白可以明显抑制人舌癌裸鼠移植瘤的生长。     

低剂量环磷酰胺联合树突状细胞瘤苗对荷舌鳞癌裸鼠的治疗观察

目的：观察低剂量环磷酰胺与树突状细胞瘤苗联合应用对裸鼠体内人舌癌细胞的抑瘤作用。方法：培养人外周血单核细胞来源的树突状细胞（DC），冻融法制备Tca8113舌癌细胞可溶性抗原并体外致敏DC，将同源人T淋巴细胞与之共同培养诱导出抗原特异性的CTL，联合低剂量环磷酰胺（Cy）共同接种至荷人舌癌的裸鼠。结果：抗原致敏的DC瘤苗与小剂量环磷酰胺联合后能比单用DC瘤苗更有效的治疗荷舌癌的裸鼠，与对照组相比，肿瘤生长明显抑制，生存率有显著提高（P〈0．05）。结论：以冻融抗原致敏的DC激活产生肿瘤特异性的CTL联合小剂量Cy能更有效的促进荷瘤宿主的免疫反应，具有显著的体内抑制舌癌的效果。     

Cal－27舌鳞癌动物模型的建立及其意义

目的：建立Cal－27舌鳞癌动物移植瘤模型,研究肿瘤细胞的生物学行为及转移瘤的特性。方法：将107／0．2 mL Cal－27细胞悬液通过在裸鼠左腋背部皮下注射接种,建立移植瘤模型。达到成瘤标准后,定期测量肿瘤大小并记录体质量变化,4周后处死裸鼠,通过组织切片HE染色法观察组织结构异型性和细胞异型性,并观察肺、肝有无转移。结果：实验裸鼠在接种后平均14天达到成瘤标准,24只裸鼠移植瘤中,成瘤率100%。未见有肺、肝脏的转移。结论：在细胞接种的数量、肿瘤接种的部位、实验动物的周龄及状态等内外部条件都适宜的情况下,成瘤率可以达到100%。该模型较客观地反应了人舌鳞癌的生物学行为,为以后的动物模型研究提供了较为适宜的注射剂量,为实验研究提供了理想的动物模型。     

裸鼠皮下种植性人头颈鳞癌模型的建立

目的：建立稳定的皮下种植性裸鼠人头颈鳞癌模型。方法：取10^9个对数期的PCI-37B细胞,种植于BALB／c裸鼠皮下。待成瘤后,从裸鼠体重、大体形态,肿瘤生长情况,病理学特征等方面进行研究。采用SPSS13．0软件包对数据进行统计学分析。结果：肿瘤形成的潜伏期约15d,实验组14只裸鼠成瘤13只,肿瘤成功率为92．8％。种植肿瘤组裸鼠体重较正常对照裸鼠明显减轻．肿瘤生长迅速．病理学检查有明显的细胞异形性。结论：成功建立了裸鼠皮下种植性头颈鳞癌模型。本模型具有在短期内易建立、稳定、重复性好、成癌率高等特点,为人头颈鳞癌的生物学、治疗学研究提供了良好的动物模型。     

尿路上皮癌胚抗原1（UCA1）在舌鳞癌中的表达及意义

目的：探讨尿路上皮癌胚抗原I（UCAl）mRNA在舌鳞状细胞癌中的表达及其与舌鳞癌病理分级和临床分期的关系。方法：采用SYBRGreenII实时荧光定量反转录聚合酶链反应实验方法．从6种基因中筛选出在40对舌鳞癌组织及配对正常舌组织样本中表达有明显差异的基因UCAl．扩大样本量至93例．检测UCAlmRNA在两者中的表达。应用SPSSl3．0软件包分析UCAlmRNA表达量与舌鳞癌患者临床病理学特征的关系。结果：UCAlmRNA在93例患者的肿瘤组织中平均表达量为0．7213,在正常舌组织中为0．2756,表达量有显著差异fP〈0．001）：UCAlmRNA的表达与患者的性别、年龄、肿瘤大小无关（P〉0．05）,与舌鳞癌的病理分级和临床分期有显著相关性（P〈0．05）。结论：UCAlmRNA高表达与舌鳞状细胞癌密切相关．对舌鳞癌的诊断、判断病理分级和临床分期具有重要意义。     

毫米波联合放射对人舌鳞癌细胞生长的抑制效应

目的 观察毫米波联合放射对Tca8113细胞集落形成及超微结构的影响。方法 采用高低功率密度毫米波联合^60Co γ射线辐射人舌鳞癌Tca8113细胞悬液,3周后观察细胞集落形成抑制率,24h后行电镜检查。结果 各实验组细胞集落形成率均有显著下降（P＜0．001）;集落形成抑制率高功率密度组高于低功率密度组（P＜0．05或P＜0．01）;长照射时间组高于短时间照射组（P＜0．05）;先后放疗组无显著差异（P＞0．05）;联合组高于单纯毫米波照射组和单纯放射组（P＜0．001或P＜0．05）。扫描及透射电镜下分别见细胞表面损伤性改变,内部结构退行性变化。高功率密度组改变程度较低功率密度组严重。结论 毫米波辐射能明显抑制Tca8113细胞的集落形成能力,并造成细胞表面形态及内部结构的改变。     

Evidence of the validity of a model of determinants of quality of restorative dental care

A model describing the relationship between self-reported quality of restorative dentistry and dentist characteristics for 119 Montana general dentists is presented. The best predictors formed a significant model explaining 22% of the variance of the quality measure. Results are contrasted with a previous estimation of the model for 102 Washington general practitioners. Evidence for the external validity of the model is presented.     

Experimental evidence of formation of imines in the course of reduction of hydrazones

The reduction of hydrazones is generally suggested to proceed through a reductive cleavage of the nitrogen–nitrogen bond followed by a reduction of the carbon–nitrogen bond. This sequence of reduction processes is here supported for fluorenone (V) and benzophenone (VI) hydrazones as well as by a comparison of the reduction of fluorenone and benzophenone hydrazonium ions (I,III) with corresponding imines (II,IV). Another proof of the presence of imines as intermediates is the splitting of four-electron waves of hydrazones V and VI and hydrazonium ions I and VIII into two waves at pH < 2. This has been interpreted as due to differences in slopes dE_1/2/dpH and pK_a-values of protonated hydrazine derivatives on one side and corresponding imines on the other. In this pH-range imines formed in reductions of VI and VIII are reduced in a single two-electron wave, those of I and V in two one-electron steps. Fluorenone imine (II) is sufficiently stable to allow recording of time-independent current–voltage curves between pH 6 and 11. In this pH-range the imine (II) is reduced in two one-electron steps. Benzophenone imine (IV) has been found stable between pH 4.6 and 12. At pH 4.6–8 the reduction of the imine IV takes place in a single two-electron step, at pH 8–12 in two one-electron steps. Final proof of the initial cleavage of the N–N bond is presented by comparison with the reduction of nitrones.     

不同剂型玻璃离子水门汀溶解性比较研究

目的:研究、比较不同剂型玻璃离子水门汀的溶解性和表面微观形态改变,为临床使用提供依据.方法:将3M树脂加强型玻璃离子水门汀(水粉剂型)、GC玻璃离子水门汀(水粉剂型)及GC玻璃离子水门汀(双糊剂型)分别在人工唾液中浸泡30 d,冷热循环15000次,烘干测重,比较前后质量变化,计算溶解率,并用扫描电镜观察表面微观改变.结果:不同剂型的玻璃离子水门汀溶解率由高到低分别为3M树脂加强型玻璃离子水门汀(水粉剂型)、GC玻璃离子水门汀(水粉剂型)、GC玻璃离子水门汀(双糊剂型).3种玻璃离子水门汀经浸泡溶解后,SEM扫描表面微观形态可观察到GE玻璃离子水门汀(双糊剂型)表面形态改变较少,其他2组玻璃离子水门汀表面微观改变较多.结论:双糊剂型玻璃离子水门汀理化性能及溶解率均低于传统水粉剂型,是未来临床修复治疗的的良好选择.     

Evaluation of efficacy of chemiluminescence for diagnosis of leukoplakia

ObjectiveLeukoplakia is the most common potentially malignant disorder preceding oral cancer. Chemiluminescence has been developed as an adjunct to conventional examination for the diagnosis of these potentially malignant disorders. This study was conducted to assess the efficacy of chemiluminescence in the diagnosis of leukoplakia and to compare the results with histopathological examination.Study designA total of 50 patients with leukoplakia were included from the outpatients attending the Department of Oral Medicine and Radiology, Dental Hospital, Bengaluru, Karnataka, India. These patients were subjected to conventional oral examination followed by chemiluminescent examination with Vizilite (Zila, Fort Collins, CO, USA) and biopsy for histopathological confirmation.ResultsThe sensitivity, specificity, positive predictive value, and negative predictive value of chemiluminescence were 93.75%, 55.56%, 78.95%, and 83.3%, respectively. The overall accuracy of chemiluminescence was 80%. A statistically significant association was observed between histopathology results and chemiluminescence results.ConclusionAlthough it is an easy, safe, minimal time consuming, and noninvasive technique, it has only adjunctive utility and it does not replace biopsy for the diagnosis of leukoplakia.     

颌骨动静脉畸形的栓塞治疗

目的：总结直接穿刺结合经血管内介入栓塞治疗颌骨动静脉静脉畸形的经验。方法：收治凳骨动静脉畸形患者6例,均进行了介入栓塞治疗。采用的栓塞材料为附凝血棉纤毛的螺圈,聚乙烯醇泡沫微粒和二氰基丙烯酸对丁酯。数字减影颈动脉造影在PHILIPSV300下完成。结果6例颌骨动静脉畸形患者中4,例急性出血得到了快速、有效控制,1例慢性渗血的右下 骨动静脉畸形患者,介入栓塞治疗,拔除松动的右下凳第一磨牙,有效地控制了出血,另1例伴局部软组织搏动性膨隆的上凳骨动静脉畸形患者,介入治疗后膨隆的搏动性得到明显改善,栓塞治疗后分别随访3－24个月,均未发现有口腔内渗血或出血。随访的X线片上,病灶区可见新骨形成。结论：局部穿刺结合经血管内介入栓塞治疗颌骨动静畸形是一种安全、有效的治疗方法。     

正畸患者切牙影像失真发生情况分析

目的研究正畸患者曲面体层片上的切牙影像失真发生情况，并分析其原因。 方法从中山大学附属口腔医院放射科影像数据库中选取500例正畸患者的曲面体层片和头影测量侧位片，所有曲面体层片均采用咬合杆投照，分别从切牙牙体影像放大、缩小、牙根变短、根尖模糊等评价指标分析上下颌切牙影像失真的发生情况，在头影测量侧位片上测量中切牙根尖-对颌切牙切缘的距离，探讨切牙影像失真发生的原因。采用SPSS 19.0统计软件对所得数据进行统计学检验。 结果500例患者中，切牙牙体影像正常者共417例，切牙牙体影像失真者共83例，影像失真发生率16.6%，其中切牙牙体影像放大17例、牙体影像缩小0例、牙根变短30例，牙根影像变短伴模糊36例。影像失真患者的根尖-切缘距离大于影像正常的患者，差异有统计学意义（F = 5 187.18，P = 0）；影像失真患者的覆盖值大于影像正常的患者，差异有统计学意义（F>477，P = 0）。 结论严重牙颌面畸形如反 、深覆盖是导致曲面体层片的切牙影像失真的主要原因之一。     

Design of clinical trials of traditional therapies of periodontitis

The present paper on the design of clinical trials of periodontal therapy first addresses the issue of the etiology of periodontal disease. It is suggested that most if not all forms of destructive periodontal disease are caused by microorganisms and that there are different forms of disease with different microbial etiologies. The progressive nature of destructive periodontal disease is subsequently discussed and it is emphasized that, in a given patient, periodontal sites which show signs of inflammation and attachment loss may not over a period of several months and years show further sign of attachment loss. The present methods of assessing periodontal disease do not allow us to discriminate between potentially active and inactive sites in untreated patients. The significance and variability of indicators of periodontal disease such as bleeding on probing, probing pocket depth and probing attachment level measurements are discussed. The errors inherent in the various measurements are analyzed and suggestions are presented describing how alterations in any of the above parameters could be identified and presented in a clinical trial. Of concern for the statistical analysis of clinical data of periodontal disease is the definition of the "experimental unit". For a number of years, the "experimental unit" in periodontal trials was the patient. It is clear, however, that different sites within the same individual show different patterns of disease progression and lesion morphology and often respond differently to periodontal therapy. Statistical analyses must consequently be designed which recognize differences in site-to-site infection and lesion morphology within a common host. Until such analyses are available, the investigator should be wary of pooling data within the same individual, since such pooling may obscure meaningful alternatives which may take place in individual periodontal sites. Some goals of periodontal therapy are subsequently identified. 4 goals are discussed more in detail, namely: to establish conditions which will allow the patient to maintain a dentition without further breakdown of the periodontium; to reduce pocket depth to establish an anatomy in the dentogingival region which with proper maintainance care will prevent the re-establishment of the subgingival infection; to gain attachment as a result of treatment; to assess the effect of a certain chemotherapeutic agent on periodontal disease.     

正常青年Monson球面半径的测量研究

目的测量正常青年Monson球面半径。方法选择60名(男30名,女30名)正常青年制取全口印模,应用立体摄影成像的原理与方法对Monson球面半径进行测量和统计学处理。结果Monson球面的半径平均为10.173 cm,大于理论值10.160 cm,差异有显著性(P<0.01);男、女性球面半径差异无显著性。结论本实验所得到的数据可作为全口义齿修复中记录颌位关系的一个参量。     

鼻测量法的进展

唇裂术后继发畸形是指唇裂修复术后,仍遗留或继发于手术操作和生长发育变化而表现出来的一类畸形[1]。包括唇畸形、鼻畸形和颌骨畸形。其修复较原发性唇裂修复更复杂,更灵活多变。而导致其修复复杂性的一个重要原因即是局部组织结构复杂变异和缺乏可靠的三维测量手段[2],鼻畸形     

口底癌34例临床分析

目的探讨口底癌的临床特性、治疗方法及预后。方法对我院自1992—2002年住院治疗的34例口底癌患者进行回顾性分析。结果34例口底癌患者中,男28例(82.4%),女6例(17.6%),男女比为4.7∶1,平均发病年龄58岁。发病部位:前口底22例(64.7%),后口底12例(35.3%)。淋巴结转移率41.2%。单纯手术组、化疗加手术组、放疗加手术组、化疗加手术加放疗组的5年生存率分别为45.5%、60.0%、50.0%、62.5%。结论口底癌以中老年患者好发,男性居多。易发生淋巴结转移,综合疗法疗效较好。