肝病治疗仪治疗慢性肝炎疗效观察

[目的]观察DSGⅠ型电脑肝病治疗仪治疗慢性病毒性肝炎的疗效。[方法]选择慢性病毒性肝炎40例,随机分为治疗组和对照组。对照组以西药常规治疗,治疗组在常规治疗的基础上加用DSGⅠ型电脑肝病治疗仪照射肝区,观察治疗后临床症状及肝功能指标等变化。[结果]治疗组和对照组两组治疗后比较血清丙氨酸氨基转移酶(ALT)、血清胆红素(Tbil)、白蛋白(ALB)有显著性差异(P<0.05),治疗组ALT、TBil较对照组恢复更加明显,治疗组ALB较对照组明显提高。治疗组与对照组比较乏力、纳差、恶心改善情况有显著性差异(P<0.05或0.01)。[结论]肝病治疗仪配合药物治疗慢性乙型肝炎可明显提高疗效。     

DSG-Ⅰ型肝病治疗仪辅助治疗病毒性肝炎的临床观察及护理

目的评价药物配合DSG—Ⅰ型肝病治疗仪治疗病毒性肝炎的疗效。方法随机将135例病毒性肝炎患者分为两组，观察组65例药物配合肝病治疗仪治疗，对照组70例采用常规药物治疗，比较两种方法的疗效。结果治疗20天后，观察组总有效率90．77％，对照组总有效率78．57％，两者有显著性差异（P〈0．05）。结论药物配合DSG—Ⅰ型肝病治疗仪治疗病毒性肝炎有效且简便。     

慢性乙肝应用 DSG－Ⅲ型红外肝病治疗仪辅助治疗临床效果观察

目的：观察慢性乙型肝炎（慢性乙肝）应用 DSG－Ⅲ型红外肝病治疗仪辅助治疗的临床效果。方法选取120例慢性乙肝患者,随机分为观察组和对照组,各60例。两组均给予保肝、降酶等治疗12周,观察组在以上用药基础上给予 DSG－Ⅲ型红外肝病治疗仪辅助治疗2个疗程。结果两组患者谷丙转氨酶（ALT）、谷草转氨酶（ AST）治疗后均较治疗前下降（ P ＜0．01）,观察组较对照组下降更为明显（ P ＜0．01）,观察组乙肝病毒脱氧核糖核酸（HBV DNA）与乙型肝炎 e 抗原（HBeAg）转阴率、ALT 和 AST 复常率均高于对照组（ P ＜0．05）;并发症发生率观察组为6．67％,对照组为1．67％,差异无统计学意义（ P ＞0．05）。结论DSG－Ⅲ型红外肝病治疗仪辅助治疗慢性乙肝有助于改善肝功能及促进病毒转阴,无明显不良反应,疗效显著,可在临床推广应用。     

DSG-I型肝病治疗仪治疗慢性乙型肝炎临床观察

目的:观察DSG-I型电脑肝病治疗仪治疗慢性病毒性乙型肝炎的疗效。方法:选择慢性病毒性乙型肝炎207例,随机分治疗组和对照组。对照组以西药常规治疗,治疗组在上述基础上加肝病治疗仪,观察治疗后症状。结果:治疗后治疗组、ALT、TBil较对照组恢复更明显。结论:肝病治疗仪配合药物治疗慢性乙型肝炎可明显提高疗效。     

联合电脑肝病治疗仪治疗病毒性肝炎的临床观察

目的 探讨在病毒性肝炎的治疗中联合使用WLGY801型电脑肝病治疗仪治疗的临床效果及护理方式。方法 采取临床随机分组病例对照试验方法。治疗组95例病毒性肝炎，在常规保肝治疗基础上加用WLGY801型电脑肝病治疗仪治疗，每日1次。每次32min，10d为1疗程，连续治疗2～3疗程。对照组95例病毒性肝炎，采用常规保肝治疗。分别观察各组临床主要症状、检测肝功能及血脂。结果 联合WLGY801型肝病治疗仪治疗组常规保肝治疗的消化道症状缓解时间、黄疸消退时间和转氨酶恢复正常时间显著缩短，优于对照组（P〈0．05或0．01）。结论WLGY801型电脑肝病治疗仪对病毒性肝炎的治疗有良好辅助作用。     

高压氧治疗对病毒性脑炎患者的疗效观察及其对血清NSE含量的影响

【目的】探讨高压氧治疗对病毒性脑炎患者脑电图变化及血清神经元特异性烯醇化酶（NSE）的影响。【方法】将60例病毒性脑炎患者随机分为两组：对照组30例，以药物治疗为主，辅以理疗康复治疗：高压氧治疗组30例，在药物和康复治疗的基础上，加用高压氧治疗。对患者的临床转归、脑电图改变及血清NSE的变化进行观察、检测及分析。【结果】1．临床疗效：高压氧治疗组的总有效率为97％，明显高于对照组的总有效率（73％，P〈0．05），高压氧组住院天数明显短于对照组（21．50＋6．02天及30．27＋10.17，P〈0．001）。2．脑电图变化：高压氧治疗组及对照组治疗前脑电图异常的发生率为100％，两组比较无显著性差异（P〉0．05）。治疗后高压氧组脑电图的好转率明显高于对照组（P〈0．05）。3．血清NSE含量的变化：两组患者治疗前血清NSE的含量均明显高于正常人（P〈0．001），治疗后高压氧组患者血清NSE的含量明显低于对照组（P〈0。001）。【结论】高压氧对病毒性脑炎具有明显的疗效．通过缩短病人的住院天数、改善患者异常的脑电图及降低血清NSE含量，表明早期进行高压氧治疗能提高治愈率．降低病残率。     

复方中药对慢性萎缩性胃炎细胞增殖抑制及PCNA、p53表达的影响

【目的】观察胃萎Ⅰ号治疗慢性萎缩性胃炎的临床效果,并从细胞增殖凋亡和相关基因的表达方面探讨其作用机理。【方法】120例患者均经胃镜与病理检查证实为慢性萎缩性胃炎,分成两组,分别采用胃萎Ⅰ号、三九胃泰冲剂治疗,免疫组化检测PCNA、p53的表达。【结果】治疗组（胃萎工号）综合疗效优于对照组（三九胃泰）（P〈0．05）,治疗组症状缓解总有效率为73．24％,对照组症状缓解总有效率48．26％,两组相比显著性差异（P〈0．01）。治疗组细胞增殖指数、PCNA和p53表达率分别从20．8％、78．05％、40．91％下降至6．5％、58．54％、13．64％（P〈0．05）,对照组上述指标无明显变化。【结论】胃萎Ⅰ号能有效治疗慢性萎缩性胃炎,其机理可能是通过抑制PCNA和p53表达,抑制过度增殖,诱导胃黏膜细胞凋亡。     

恩替卡韦治疗慢性乙型重型肝炎临床观察

目的观察在内科综合治疗的基础上,用恩替卡韦治疗慢性乙型重型肝炎的疗效及安全性。方法37例慢性重型乙型肝炎患者随机分成为两组,治疗组20例患者在内科综合治疗的基础上加用恩替卡韦0．5mg/d治疗,对照组17例患者仅内科综合治疗。在治疗前和治疗6～8周后分别检测两组患者血清丙氨酸氨基转移酶（ALT）、总胆红素（TB）、白蛋白（ALB）、凝血酶原活动度（PTA）和HBV DNA。如患者治疗后症状消失或缓解,上述检查结果恢复者判断为有效,反之为无效。结果治疗组20例患者治疗有效14例,有效率为70％,对照组17例患者治疗有效6例,有效率为35．9％。两者比较有统计学意义（P〈0．05）。治疗组患者经6～8周治疗后患者血清ALT、TB和HBV DNA较治疗前明显下降（P〈0．05）,ALB和PTA较治疗前明显上升（P〈0．05）,均有统计学意义。对照组患者经6～8周治疗后患者血清ALT、TB较治疗前下降,但无统计学意义（P〉0．05）;HBV DNA、ALB和PTA较治疗前变化均无统计学意义（P〉0．05）。治疗组患者口服恩替卡韦0．5mg／d治疗6～8周后未出现不良反应。结论在内科综合治疗的基础上,恩替卡韦治疗慢性重型乙型肝炎安全且有效。     

DSG-Ⅲ型生物信息红外肝病治疗仪治疗慢性肝病31例疗效观察

目的:评估DSG-Ⅲ型生物信息红外肝病治疗仪(BILT肝病治疗仪)治疗慢性肝病的临床疗效。方法:选择慢性肝病60例,随机分为治疗组和对照组。对照组(n=29)以药物常规治疗,治疗组(n=31)在常规治疗的基础上加用BILT肝病治疗仪照射肝区(治疗时间为30 min,2次/d),在治疗前及治疗2周后,观察两组临床症状(乏力、纳差、腹胀、肝区不适)及肝功能指标(包括ALT、AST、DBIL、IBIL、γ-GT、ALP、ALB、GLB)的变化。结果:与治疗前比较,治疗组肝功能ALT、AST、DBIL、IBIL、ALP、γ-GT均显著降低(P<0.05),但ALB、GLB变化无统计学差异。而对照组中ALT、AST、DBIL、IBIL亦下降明显,但γ-GT、ALP、ALB、GLB变化无统计学差异。治疗后两组ALT比较有统计学差异[(42±28)IU/L与(86±71)IU/L(P<0.01)],治疗组ALT较对照组恢复更加明显。从临床症状,如乏力、纳差、肝区不适等来看,治疗组改善的例数和比率均优于对照组,差异有统计学意义(P<0.05)。而腹胀缓解方面,两组差异无统计学意义(P>0.05)。结论:BILT肝病治疗仪配合药物较单纯药物治疗慢性肝病可提高疗效,缓解患者部分临床症状。     

优思弗治疗胆汁淤积性肝病64例临床观察

目的观察优思弗对胆汁淤积性肝病的疗效。方法128例胆汁淤积性肝病病人随机分为治疗组和对照组，每组各64例，对照组给予甘利欣及常规护肝治疗，治疗组在与对照组相同治疗的基础上再给予优思弗250mg／次，3次，d，po，疗程均为8wk。结果治疗后两组在症状恢复方面比较：纳差、乏力症状有明显改善，但差异无显著性（P〉0．05）；而皮肤瘙痒差异有显著性（P〈0．05）。体征复常方面比较：肝、脾肿大差异无显著性（P〉0．05）；而胆囊壁增厚、水肿差异有显著性（P〈0．05）。治疗组治疗后肝功能指标下降幅度均明显优于对照组，差异有显著性，其中总胆红素（TBi1）、直接胆红素（DBi1）、胆汁酸（TB）与对照组比较，P〈0．01；丙氨酸氨基转移酶（ALT）、碱性磷酸酶（ALP）、γ-谷氨酰转移酶（T-GT）与对照组比较P〈0．05。根据疗效判定标准，总有效率为93．75％，与对照组比较P〈0．01。结论优思弗治疗胆汁淤积性肝病疗效显著，可作为胆汁淤积性肝病的首选药物。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.