As2O3诱导Raji细胞凋亡的基因分析

Objective The effect of arsenic trioxide on apoptosis gene expression of Raji cell was explored when Raji cells were incubated with 0.5μmol/L of arsenic trioxide for 6h。Methods Cell culture,extraction and isolation of mRNA,preparation of probes labeled with fluorescence,hybridization technique of DNA chip(each chip containing 200 apoptosis genes,Chinese Shanghai Biostar,In.)were used.Results Arsenic trioxide induced significant changes in 10%(20/200 genes)of the apoptosis genes:18 genes were downregulated,only two upregulated.In particular,inhibitors of apoptosis protein,such as X-linked inhibitor of apoptosis protein,were significantly downregulated.P53 and the other apoptosis genes were also downregulatec.Of the upregulated genes,high expression of heat-shock protein could promote apoptosis of Raji cells.Conclusion The inhibitors of apoptosis protein play an important role in the process of arsenic trioxide-induced apoptosis of Raji cells.     

Effect of Celecoxib on Apoptosis of Endometrial Carcinoma Cell

Objective:To investigate the effect of Celecoxib on proliferation and apoptosis of the endometrial carcinoma cell HEC-1B and the effect on the expression of Fas and Survivin mRNA.Methods:The inhibition on the growth of human endometrial carcinoma cell HEC-1B was investigated by cell culture and MTT experiment when treated with different concentrations of Celecoxib.The cell apoptosis was detected by flow cytometry and DNA Ladder Electrophoresis.The change of the expression of Fas and Survivin mRNA after the treatment of Celecoxib was detected With RT-PCR.Results:Celecoxib could effectively inhibit the growth of HEC-1B cells and induce apoptosis.Survivin mRNA expression was decreased and Fas mRNA expression was increased after treating with Celecoxib.Conclusion:Celecoxib could inhibit HEC-1B cell proliferation and induce its apoptosis.     

The effect and significance on Akt and PTEN expression in melanoma B16 cells with chamaejasme extract

Objective： The aim of this study was to investigate the effect on Akt and PTEN expression and the mechanism of proliferation and apoptosis of melanoma B16 cells treated with chamaejasme extract. Methods： The expressions of Akt and PTEN of B16 cells treated with different concentrations of chamaejasme extract were detected with immunohistochemical method, cell apoptosis index （AI） was calculated with in situ labeling method. Results： Akt expressions of B16 cells treated with different concentrations of chamaejasme extract were significantly lower than the control group, while the PTEN expres- sions significantly up-regulated, and the effects appeared to be dose-related （P 〈 0.05）. The Akt/AI of B16 cells treated with different concentrations of chamaejasme extract was significantly lower than the control group, all the parameters had ex- tremely difference （F = 24.58, P 〈 0.05）. Conclusion： Chamaejasme extract can inhibit proliferation and induce apoptosis of malignant melanoma B 16 cells by down-regulating the expression of Akt and up-regulating the expression of PTEN.     

仙人掌原液毒性的初步研究

背景与目的： 研究仙人掌原液的毒性。 材料与方法： 小鼠急性毒性试验、Ames试验、小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验、大鼠30 d喂养试验。 结果： 仙人掌原液雌、雄小鼠LD50均大于20.0 g/kg,属无毒物质;Ames试验、微核试验和精子畸形试验结果均为阴性;大鼠30 d喂养试验结果显示该样品30 d喂养对大鼠各项观察指标未见毒性作用。结论： 在本次实验条件下,仙人掌原液为无毒物质,未显示有遗传毒性和亚急性毒性作用。     

食管平滑肌瘤10例临床治疗体会

我院１９７５年６月～１９９０年７月共收治食管平滑肌瘤１０例，占同期食管肿瘤总数的０．１９２％（１０／１０９２）。位于食管上段２例，中段５例，下段３例。Ｘ线食管钡餐造影是诊断本病的主要方法。行食管粘膜外肿瘤摘除９例，食管部分切除１例，效果良好。本文就其诊断与手术治疗进行了讨论。     

中晚期贲门癌148例的外科治疗

目的　总结贲门癌手术治疗影响生存率的因素 ,提供今后工作参考。方法　对 14 8例经手术治疗的贲门癌患者进行术后并发症、绝对生存率的X2 检验。结果　本组切除率为 94.5 9% ,近半胃切除占 72 .14 % ,1、3、5年生存率分别为 67.9%、45 %和 2 4.3 %。病期、外侵程度和淋巴结转移等因素对 5年生存率有显著影响 (P <0 .0 1)。术后并发症以吻合口瘘及肺癌并发症为多见 ,其发生率为 3 .6% ,手术死亡率 1.4%。结论　提高生存率的关键在于早期诊断 ,根治手术和术后积极综合治疗。     

胆囊癌29例分析

目的探索胆囊癌的防治方法。方法分析29例胆囊癌病人临床资料、治疗方法及结果。结果术前诊断明确者21例，剖腹探查发现已属晚期，9例为Ⅳ期行根治术，12例为Ⅴ期未行根治术，均于术后1年内死亡。术前怀疑胆囊癌者5例，剖腹探查冰冻切片证实为Ⅴ期及Ⅳ期者各1例，Ⅴ期未行根治术，Ⅳ期行根治术，均于术后1年内死亡；Ⅲ期者3例，行胆囊癌根治术分别于术后10月、13月、17月死亡。2例术中冰冻切片发现的Ⅱ期胆囊癌，行胆囊癌根治术，分别于术后23月、26月死亡。1例意外胆囊癌属Ⅰ期胆囊癌，术后5年半死亡。结论要减少胆囊癌危害，重在及时治疗胆囊结石。     

仙人掌原毒性的初步研究

背景与目的:研究仙人掌原液的毒性。材料与方法:小鼠急性毒性试验、Ames试验、小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验、大鼠30 d喂养试验。结果:仙人掌原液雌、雄小鼠LD50均大于20.0 g／kg,属无毒物质;Ames试验、微核试验和精子畸形试验结果均为阴性;大鼠30 d喂养试验结果显示该样品30 d喂养对大鼠各项观察指标未见毒性作用。结论:在本次实验条件下,仙人掌原液为无毒物质,未显示有遗传毒性和亚急性毒性作用。     

Management of intracranial metastases of differentiated carcinoma of thyroid

Summary Brain metastases in differentiated carinoma of the thyroid is a rare occurrence. We treated five documented cases of carcinoma of thyroid with brain metastases out of 400 cases of thyroid cancer treated between 1972 to 1993. 4 were females out of which one was pregnant during the appearance of brain metastases. All cases were treated with thyroidectomy, and radioiodine as primary therapy. Brain metastases developed 6 months to 11 years following treatment of the primary and were treated with radiotherapy and suppressive levothyroxine. We observed the beneficial effect of suppressive thyroxine and the poor prognosis associated with pregnancy and withdrawl of thyroid replacement therapy. 3 of the 5 patients are alive 12–23 months after treatment for brain metastases, while 2 patients died at 4 months and 7 years post brain metastases due to pulmonary and hepatic failure, respectively.     

乳腺叶状囊肉瘤320例诊治分析

目的探讨乳腺叶状囊肉瘤临床诊断和治疗方法。方法回顾分析经手术病理确诊为乳腺叶状囊肉瘤的320例患者的诊断和治疗情况。结果行乳腺X片检查121例,B超检查25例,近红外检查21例,针吸细胞学检查42例;15例(12.4%)乳腺X片检查及3例(14.3%)近红外检查诊断为乳腺叶状囊肉瘤。临床诊断正确率为17.5%(56/320),误诊率为82.5%(264/320)。随访253例患者,其中局部复发45例(17.8%),远处转移死亡13例(5.1%)。结论乳腺叶状囊肉瘤诊断需常规病理检查,治疗宜行局部广泛切除或全乳切除。     

胆囊腺瘤癌变的相关因素分析

目的:探讨胆囊腺瘤癌变与临床因素的相关性。方法:应用ROC曲线,计算胆囊腺瘤癌变的肿瘤大小临界值。应用χ2独立性检验,计算胆囊腺瘤癌变与临床因素的相关性、临床表现与肿瘤位置的相关性。结果:肿瘤良恶性临界值为2.27 cm。胆囊腺瘤癌变与患者的性别、年龄、是否合并结石无明显相关性（P＞0.05）,而与肿瘤大小、肿瘤数目、形态、超声下是否有血流(P＜0.05)等临床病理特征有明显相关性。临床症状与肿瘤是否位于胆囊颈部有明显相关性(P＜0.05)。结论:胆囊腺瘤在超声扫描下表现为单发、无蒂、有血流、直径大于2.27 cm,应高度怀疑已有恶变。肿瘤位于胆囊颈部可引起右上腹胀痛、疼痛向右背部放射、恶心呕吐等临床表现。     

恶性肿瘤肝转移假性肝硬化发生机制及诊疗的研究进展

随着对恶性肿瘤的深入研究,越来越多的肿瘤患者接受到更规范更多线的治疗.更多线的化疗及靶向治疗,使一部分恶性肿瘤肝转移患者的肝脏发生类似肝硬化的形态学及临床改变,称之为假性肝硬化.由于假性肝硬化罕见,对于它的认识多来自于个案报道.因此,本文通过综述国内外关于假性肝硬化的个案报道及相关研究,对其发生机制、影像学及临床表现和...