消臌方治疗乙肝肝硬化腹水临床观察

目的观察中药消臌方联合基础治疗乙型肝炎肝硬化（以下简称乙肝肝硬化）腹水的疗效。方法采用益气活血利水法配合常规西药治疗乙肝肝硬化腹水32例为治疗组,并设单纯西药治疗32例为对照组。结果治疗组有效率87．5％明显高于对照组的70．0％（P〈0．05）。在腹水消退,白蛋白、T细胞亚群、症候积分的改善均明显优于对照组（P均〈0．05）。结论中药消臌方联合基础治疗对肝硬化腹水的疗效明显优于单纯西药治疗。     

益气活血平肝汤治疗高血压肾病患者临床疗效及血同型半胱胺酸影响

目的：观察中药益气活血平肝汤结合常规西药对高血压肾病（HN）患者的临床疗效及血同型半胱胺酸（tHcy）的影响。方法：选择HN患者100例进行前瞻性随机对照临床试验。治疗组和对照组各50例,治疗组给予西药常规治疗基础上加益气活血平肝汤加减治疗,对照组用西药常规治疗,疗程均为2个月。观察患者I瞄床疗效,并检测治疗前和治疗2个月后血同型半胱胺酸含量、24h尿蛋白定量、血肌酐、尿素氮水平。结果：总有效率治疗组76％,对照组52％,两组比较,差异有显著性意义（P〈0．05）;治疗两组血同型半胱胺酸（tHcy）含量分别与治疗前比较,差异均有显著性意义（P〈0．05）,两组治疗后24h尿蛋白定量、血肌酐、尿素氮比较,差异均有显著性意义（P〈0．05）。结论：益气活血平肝汤结合常规西药能有效改善HN的临床症状,降低血同型半胱胺酸含量,降低24h尿蛋白定量、血肌酐、尿素氮水平。     

特利加压素对Ⅰ型肝肾综合征的疗效评估

目的观察特利加压素治疗肝硬化失代偿期Ⅰ型肝肾综合征的疗效。方法选取60例Ⅰ型肝肾综合征患者,随机分成两组,每组各30例,在常规治疗基础上随机选择一组使用特利加压素,另一组为对照组,治疗期间分别检测患者血肌酐、尿素氮、肌酐清除率、尿量、腹围等指标变化情况。结果特利加压素组患者临床症状明显改善,尿量、肌酐清除率增加,肌酐降低,胱抑素C降低,腹水减少,尿钠、尿钾,与对照组比较,差异有统计学意义（P〈0．05）。结论特利加压素对Ⅰ型肝肾综合征有确切的治疗效果。     

调肝消臌汤治疗乙肝后肝硬化腹水的临床观察

目的观察调肝消臌汤治疗乙肝后肝硬化腹水的临床疗效。方法对照组30例为西医治疗,治疗组30例,采用调肝消臌汤辨证施治。两组均1个月为1个疗程,2个疗程后评价疗效。结果治疗组总有效率为83.3%,对照组为66.7%,两组比较为差异有统计学意义（P〈0.05）。本研究显示,治疗组疗效明显优于对照组（P〈0.05）,腹水消退情况治疗组与对照组比较有显著性差异（P〈0.01）。结论调肝消臌汤施治乙肝后肝硬化腹水的临床疗效优于单纯西医治疗。     

腹水超滤过浓缩后腹腔回输治疗肝硬化顽固性腹水15例报告

目的应用腹水超滤过浓缩后腹腔回输技术治疗顽固性腹水的临床研究．方法15例顽固性腹水为研究对象,在回输过程中将腹水连接引入回输机,超滤浓缩腹水后回输回腹腔．观察回输前后腹围、体重、尿量、血浆蛋白以及尿素氮,电解质钾,钠,氯的变化．结果治疗15例共20次,腹水消退率达100％．腹水回输后腹围、体重显著下降（P〈0．05）;尿量、血浆蛋白显著增加（P〈0．05）;腹水回输前后尿素氮,电解质钾,钠,氯无明显改变（P〉0．05）,无严重不良反应发生．结论腹水超滤过浓缩后腹腔回输治疗顽固性腹水的方法操作简便,见效快,疗效好,副作用少．     

养肾汤治疗慢性肾小球肾炎的临床研究

目的：观察自拟养肾汤治疗慢性肾小球肾炎的临床疗效。方法：将62例慢性肾小球肾炎患者随机分为两组,对照组30例予西医常规治疗,治疗组32例在对照组治疗基础上加用养肾汤。治疗8周后比较两组临床疗效和相关生化指标的改善情况。结果：两组临床综合疗效比较差异有统计学意义（P〈0．05）,治疗组优于对照组;两组在改善血肌酐（Scr）、血尿素氮（BUN）、尿蛋白（Upro）、血红蛋白（Hb）和尿红细胞计数（URBC）指标方面比较,差异有统计学意义（P〈0．05或P〈O．01）,治疗组优于对照组。结论：养肾汤治疗慢性肾小球肾炎有较好的临床疗效。     

灯盏花素注射液联合依那普利治疗早期糖尿病肾病效果评价

目的观察灯盏花素注射液联合依那普利治疗早期糖尿病肾病的临床疗效。方法201o年3月～2011年3月,116例早期糖尿病肾病患者随机分为两组,对照组58例采用依那普利治疗,观察组58例对照组基础上采用灯盏花素注射液治疗,比较观察两组的临床疗效及24h尿微量自蛋白、血肌酐和尿素氮水平的变化。结果观察组的总有效率明显高于对照组（P〈0．05）。两组治疗后24h尿微量白蛋白、血肌酐和尿素氮水平均较治疗前明显下降（P〈0．05）。并且,观察组治疗后24h尿微量白蛋白、血肌酐和尿素氮水平明显低于同期对照组（P〈0．05）。结论灯盏花素注射液联合依那普利对早期糖尿病肾病患者进行治疗,能够明显提高临床疗效、改善患者肾功能,值得临床推广应用。     

中西医结合治疗糖尿病肾病43例临床观察

目的：观察中西医结合治疗糖尿病肾病的临床疗效。方法：将86例糖尿病肾病患者随机分为治疗组与对照组各43例,对照组采用西医常规治疗,治疗组在对照组的基础上加用补肾活血汤。观察比较两组治疗后的综合疗效及治疗前后24h尿蛋白定量、血尿素氮（BUN）、血肌酐（SCr）值。结果：两组治疗后综合疗效比较差异有统计学意义（P〈0．05）,两组治疗后24h尿蛋白定量比较差异亦有统计学意义（P〈0．05）,两组治疗后血尿素氮（BUN）、血肌酐（SCr）值比较差异无统计学意义（P〉0．05）。结论：中西医结合治疗糖尿病肾病疗效显著,值得临床推广应用。     

腹水超滤浓缩腹腔回输对肝肾综合征的治疗价值

目的研究腹水超滤浓缩腹腔回输治疗肝肾综合征的临床疗效。方法随机选取40例经常规治疗无效的肝硬化顽固性腹水患者,进行腹水超滤浓缩腹腔回输治疗,观察治疗后24h腹围、体重、尿量、食欲及精神状态的变化,测定血钠、血浆白蛋白、血肌酐及肌酐清除率。结果采取腹水超滤浓缩腹腔回输治疗后,患者腹围缩小,体重下降,24h尿量增多,食欲及精神状态改善,血钠、血浆白蛋白、肌酐清除率上升,血肌酐下降（P〈0．05）。结论采用腹水超滤浓缩腹腔回输治疗肝肾综合征较为理想。     

大剂量缬沙坦对糖尿病肾病患者尿蛋白排泄量和肾功能的影响

目的对大剂量缬沙坦在治疗糖尿病肾病中对患者尿蛋白排泄量和肾功能的影响进行评价。方法选取2011—2012年我院收治的60例糖尿病肾病患者作为研究对象,在糖尿病治疗措施的基础上,加用口服大剂量缬沙坦f162mg／d）,对治疗前、后患者的血压、24h尿蛋白、24h尿清蛋白、24h尿β2微球蛋白、24h尿IgG、血尿素氮、血肌酐、血β2微球蛋白、血钾、空腹血糖进行观察。结果患者24h尿总蛋白、24h尿清蛋白、24h尿β2微球蛋白和24h尿IgG与治疗前相比均显著降低（P〈0．05）,收缩压、舒张压与治疗前相比也显著降低（P〈0．05）;血β2微球蛋白、血尿素氮、血肌酐、血钾及空腹血糖与治疗前差异均无统计学意义（P〉0．05）。结论应用大剂量缬沙坦治疗糖尿病肾病,可明显改善患者的蛋白尿症状和血压,对患者的肾脏有保护作用。     

补肾活血方对PCOS大鼠模型卵巢中PAI-1mRNA表达的影响

目的探讨补肾活血方对大鼠PCOS模型卵巢局部纤溶酶原激活物抑制因子-1（PAI—1mRNA）表达的影响。方法选用未成年24d龄SD雌性大鼠60只,随机分为模型组、克罗米酚组、补肾活血方高剂量组、补肾活血方低剂量组、正常对照组5组。用Bogovich法建立大鼠多囊卵巢病理模型。以克罗米酚为对照。用原位杂交法观察补肾活血方对多囊卵巢大鼠局部PAI—1mRNA的影响。结果模型组卵巢局部PAI—1mRNA存在卵泡膜间质细胞显著增高,用补肾活血方高、低剂量与克罗米酚药后,卵巢局部PAI-1mRNA的表达明显降低．差异具有统计学意义（P〈0．05、P〈0．01）。补肾活血方高剂量组与克罗米酚组比较,差异具有显著统计学意义（P〈0．01）;低荆量组与克罗米酚组比较差异无统计学意义（P〉0．05）,补肾活血方高、低剂量组比较,低剂量组卵泡膜间质细胞上PAI-1的基因表达增高更明显,但二者差异无统计学意义（P〉0．05）。结论PAI-1mRNA可能与多囊卵巢综合征的发病机制有关。以补肾活血立法的补肾活血方能降低多囊卵巢大鼠局部PAI—1mRNA的显著增高表达．降低PAI—1mRNA卵巢局部的作用。提示补肾活血方可能通过PAI—1mRNA途径促进卵巢排卵的机制。     

益智健脑颗粒联合针灸对阿尔茨海默病大鼠学习记忆的影响

目的观察益智健脑颗粒联合针灸对阿尔茨海默病（Alzheimers disease,AD）大鼠学习记忆的影响。方法将大鼠随机分为假手术组（A组）、模型组（B组）、针灸组（C组）、益智＋针灸组（D组）各10只,B、C、D 3组分别以海马CA1区注射β淀粉样蛋白25-35（Aβ25-35）造模,A组注射等量的双蒸水,各组分别治疗20 d后行Morris水迷宫试验,观察大鼠学习记忆能力变化。结果B组较A组的平均潜伏期明显延长,差异具有统计学意义（P〈0.05）;与B组比较,C组、D组的平均潜伏期明显缩短,过台次数增多,差异具有统计学意义（P〈0.05,P〈0.01）;与C组比较,D组的潜伏期缩短,过台次数增多,差异具有统计学意义（P〈0.05）。结论益智健脑颗粒联合针灸能够提高Aβ25-35介导的AD模型大鼠的学习记忆能力。     

表皮生长因子受体酪氨酸激酶抑制剂在非小细胞肺癌应用的瓶颈——获得性耐药

以表皮生长因子受体酪氨酸激酶抑制剂（epidermal growth factor receptor-tyrosine kinase inhibitor,EGFR-TKI）分子靶向治疗研究为肇始,转化性研究使基础实验和临床实践间的鸿沟迅速填平,改变着人们认识治疗肺癌的视角。无可否认,EGFR-TKI上市后极大地延伸了肿瘤学家治疗肺癌的手段,但不管从临床经验、临床研究数据、分子生物学层面还是文献计量学的角度,     

WHO西太区与“世界中医药学会联合会”中医名词术语国际标准比较研究：精、神、气、血、津、液部分

精、神、气、血、津、液是中医理论中6个非常重要的概念，有关术语在《WHO西太区传统医学国际标准名词术语》中一共收录了59条，“世界中医药学会联合会”（以下简称世中联）《中医基本名词术语中英对照国际标准》中收录了58条。血、津、液的内涵较为具体，有一定的物质基础，理解并不困难。翻译上虽有差异，但亦不难统一。精、神、     

Liuwei Dihuang,a traditional Chinese herbal formula,suppresses chronic inflammation and oxidative stress in obese rats

OBJECTIVE： To investigate the anti-inflammatory, anti-oxidative stress, and adipokine-ameliorating effects of Liuwei Dihuang （LWDH）, a traditional Chinese herbal formula, in obese rats. METHODS： After 2 weeks of acclimation with free access to regular rodent chow and water, obese-prone-caesarean-derived （OP-CD） rats were fed a modified AIN-93G diet containing 60% energy from fat. Treatment was performed twice daily by gavage feeding with 500, 1 500, or 3 500 mg/kg body weight LWDH suspended in water （n=12 rats per group）. Twelve obese-resistant-CD （OR-CD） rats were fed the atherogenic diet and gavaged with water, and served as the normal control. Blood biomarkers of inflammation, oxidative stress and adiponectin were measured post-sacrifice and used to determine the treatment effect of LWDH and assess the suitability of OR/OP-CD rats for studying these parameters. RESULTS： After 9 weeks of treatment, LWDH lowered serum C-reactive protein （CRP） and tumour necrosis factor-α （TNF-α） levels. Serum interleukin-6 （IL-6） levels showed a tendency towards reduction, but were not significantly different from the OP-CD control. Liver superoxide dismutase （SOD） activity was increased in response to all three doses of LWDH, while the levels of reduced （GSH） and oxidized glutathione （GSSG） and thiobarbituric acid reactive substances （TBARS） were unchanged. Serum adiponectin levels were increased in response to oral administration of LWDH at the dose of either 500 or 1 500 mg/kg body weight. In addition, comparisons between OR-CD and OP-CD rats revealed differential, and for some biomarkers, conflicting characteristics of high-fat diet-fed OP-CD rats in reference to obese human subjects in terms of inflammatory and oxidative stress biomarkers and circulating adiponectin levels. CONCLUSION： The results show, for the first time, the anti-inflammatory, anti-oxidative stress and adiponectin-ameliorating effects of LWDH in obese rats. The suitability of the OP-JOP-CD rat model as     

New Mechanism of Herb-herb Interaction between Ginseng and Trogopterus Based on CYPs in Rat Livers

The study was designed to investigate the potential mechanism of herb-herb interaction between ginseng and Trogopterus （Trg） based on Cytochrome P450 isozymes （CYPs） in rat livers. We estimated the influence on CYP1A2, CYP2E1, and CYP3A1/2 activity caused by ginseng and Trg used in combination. The CYP1A2 and CYP2E1 enzyme activity were induced by ginseng and Trg used in combination. And this induction effect was caused via inducing CYP1A2 and CYP2E1 protein expression which was supposed caused by inducing the gene expression of CYP1A2 and CYP2E1.     

中医疗法治疗运动性疲劳的研究进展

随着竞技体育的发展，运动员经常承受着大负荷、超强度的体力训练，因此极易产生运动性疲劳。疲劳的出现使肌内压增高，局部缺血，造成氧化代谢、H^＋排出率与pH值降低，血乳酸增高，从而影响肌纤维神经传导速度和肌内收缩力量，减弱了肌肉保护能力。致使较多的冲击力传到骨骼上，故易导致疲劳骨折的发生,严重影响了运动员的训练和比赛成绩，对运动员身心产生不必要的伤害。运动性疲劳消除手段的研究一直是竞技体育工作和运动医学关注和研究的焦点。     

Effects of tacrolimus and cyclosporine treatment on metabolic syndrome and cardiovascular risk factors after renal transplantation: a meta-analysis

Background The therapeutic success of renal transplantation has been largely attributable to the development of effective and balanced immunosuppressive treatment regimens.This study provides a meta-analysis of a series of randomized controlled trials that compared the effects of tacrolimus and cyclosporine on metabolic syndrome （MetS） and cardiovascular risk factors after renal transplantation.Methods We searched various electronic databases and bibliographies,including MEDLINE,the Cochrane Central Register of Controlled Trials,and EMBASE,for relevant studies published prior to October 2012.Results Our meta-analysis included five randomized controlled trials that examined a total of 923 patients.The tacrolimus group and the cyclosporine group exhibited no significant differences in MetS incidence after renal transplantation; risk ratio （RR）：1.06,95％ confidence interval （C/）：0.73-1.55,P=0.76.Cyclosporine treatment was associated with a higher incidence of hyperlipidemia （RR：0.50,95％ CI：0.39-0.64,P ＜0.01）.Although there were no statistically significant differences,cyclosporine treatment was associated with a higher incidence of hypertension （RR：0.91,95％ CI：0.83-1.00,P=0.06） after renal transplantation compared to tacrolimus treatment,and tacrolimus treatment was associated with a higher incidence of diabetes after renal transplantation （RR：1.79,95％ CI：0.98-3.27,P=0.06） compared to cyclosporine treatment.Conclusions Compared to tacrolimus treatment,cyclosporine treatment was associated with a higher incidence of hyperlipidemia.Future large-scale studies are expected to be conducted to further confirm our findings.     

程丑夫教授论治情志病验案举隅

程丑夫是国家级名老中医,湖南中医药大学第一附属医院主任医师、教授、博士生导师,享受政府特殊津贴专家,出身于中医世家,从医40余年,经验丰富,对于内科系统及疑难杂症的治疗颇有心得,笔者有幸跟师学习,聆听教诲,受益匪浅,现将程师论治情志病的经典验案略陈一二。1思虑伤脾案患者肖某,女,27岁。初诊：2014年5月20日。半年前因婚变后出现忧心忡忡,多思多虑,近1月来反复腹部胀满,刻诊：腹胀,食后为甚,呃逆,无反酸,通气后可减轻,无腹痛,不欲食,夜寐不安,二便调。舌红苔厚白腻,脉弦,BP：110／70mmHg。     

UniPron is A Fully Effective Non-hormonal Reversible Contraceptive in Baboon Model （Papio Anubis）

Objective To determine the safety and efficacy of UniPron as a reversible contraceptive.
Methods Vaginal swabs were obtained before and after UniPron administration, cultured onto appropriate culture media and bacteria identification was done based on type of media used, Gram stain reactions, colony morphology and biochemical tests. Vaginal biopsy tissues were processed using paraffin wax method, stained with hematoxylin and eosin and examined under light microscopy to determine the effect of the product on vaginal tissues. The effect of UniPron on sperm was examined by mixing the product with electroejaculated spermatozoa in vitro at different concentrations. For efficacy studies, male baboons of proven fertility were mated with UniPron treated or untreated females of proven fertility during the fertile stages.
Results All the five females （100%） that were treated with UniPron did not conceive and they regained total fertility when the treatment was stopped while all the controls conceived. At a concentration of 40%, UniPron completely immobilized spermatozoa in an in-vitro system. UniPron mechanism of action was by lowering the vaginal pH and on application in baboon, the pH was lowered for at least 3 h after which it went back to normal.
Conclusions As we plan for a study to test UniPron as a microbicide to prevent STIs including HIV, our current study has established that this novel product is effective in contraception and harmless to vaginal tissues and vaginal microbial flora in a baboon model （Papio anubis）.