贯叶连翘提取物对血管性痴呆大鼠学习行为和组织病理学的保护作用

目的：研究贯叶连翘提取物防治血管性痴呆的作用。方法：永久性结扎大鼠双侧颈总动脉，采用水迷宫和病理组织学方法观察药物作用。结果：贯叶连翘提取物能降低永久性结扎双侧颈总动脉后24小时内大鼠的死亡率，有降低大鼠水迷宫游出时间和错误次数的趋势，给药30d给药组与对照组相比水迷宫的错误次数显著降低；能防止海马锥体细胞死亡；提高大鼠双侧颈动脉永久性结扎后，海马SS阳性神经元数目。结论：贯叶连翘提取物在治疗血管性痴呆上有一定的开发前景。     

The inhibitory effect of Hypericum perforatum extract on morphine withdrawal syndrome in rat and comparison with clonidine

The effects of Hypericum perforatum (St John's wort) on morphine withdrawal syndrome and comparison with clonidine have been investigated in morphine‐dependent rats. Adult male Wistar rats were subjects. The frequencies of withdrawal behavioral signs (rearing, teeth chattering and jumping) induced by naloxone challenge were demonstrated in morphine dependent rats receiving Hypericum perforatum extract (HPE), saline or clonidine. The withdrawal behavioral manifestations in rats were inhibited significantly by chronic co‐administration of Hypericum perforatum extract or clonidine with morphine. This study showed that clonidine was more effective than HPE at a dose of 0.4 mL/200 g and there was no significant statistical difference between the mean frequency of withdrawal signs of HPE at a dose of 0.8 mL/200 g compared with clonidine (0.2 mg/kg i.p.) but at a dose of 1.2 mL/200 g of HPE was significantly stronger than clonidine in attenuation of the morphine withdrawal syndrome. The findings suggest that HPE is capable of reducing the symptoms of opiate withdrawal and its effectiveness may be equivalent to clonidine in reducing the opiate withdrawal syndrome and may have human therapeutic potential. Copyright © 2009 John Wiley & Sons, Ltd.     

Hepatoprotective activity of Hypericum perforatum L. alcoholic extract in rodents

In recent years, interest in the hepatoprotective plants and plant based chemicals is increasing. In Turkish folk medicine, Hypericum perforatum L. has been used for various purposes including liver protection. The hepatoprotective effect of H. perforatum was investigated in vivo by cannulating the rat bile duct for choleretic activity and by barbiturate sleeping time following CCI₄-induced hepatic injury. The increase in the bile secretion following intraduodenal injection of H. perforatum extract, and the appearance of its constituents in the bile were observed by its colour change and this observation was confirmed by thin layer chromatographic analysis suggesting the excretion of hypericin into the bile as well. It was observed that the extract shortens the barbiturate sleeping time of CCI₄-treated mice suggesting hepatoprotection.     

HPLC法测定贯叶连翘中贯叶金丝桃素含量

目的：测定贯叶连翘中贯叶金丝桃素的含量。方法：采用HPLC法,流动相：乙腈－水（140：1,内含1％冰醋酸）,流速：1mL/min,检测波长276nm,结果：该法的平均回收率为98．47％,RSD＝1．33％。结论：方法可用于贯叶金丝桃药材及其制剂 质量控制。     

贯叶连翘提取物抗抑郁作用研究

目的：研究贯叶连翘提取物对小鼠的抑制郁作用。方法：选取了强迫小鼠游泳实验，小鼠尾悬吊应激实验，拮抗利血平所致的抑制症状等实验指标。结果：贯叶连翘提取物150mg/kg,300mg/kg显著缩短迫游泳小鼠及尾悬吊小鼠的不动时间；显著拮抗利血平所致的体温下降作用和小鼠眼睑下垂作用。结论：贯叶连翘提取物具有一定的抗抑郁作用。     

Development of pregnancy in rats treated with Hypericum perforatum

St John's wort (Hypericum perforatum) is a medicinal plant used in the treatment of depression and other psychiatric disorders. In the present paper, the toxicity of H. perforatum administered to female rats during the period of organogenesis (day 9-15 of pregnancy) was evaluated. Thirty inseminated Wistar rats were randomly distributed into control and treated groups, which received, by gavage, 0.5 mL of saline and 36 mg/kg body weight of Jarsin dried extract diluted into 0.5 mL of saline, respectively. Maternal toxicity was evaluated through: water and food intake, body weight gain, piloerection, locomotor activity, diarrhea and death occurrence. Animals were killed on day 21 of pregnancy, when fetuses and placentas were removed and weighed. The indices of implantation and resorption were calculated. Clinical signs of maternal toxicity were not observed and none of the variables analysed showed statistically significant differences. In the dose administered in the experimental model used, H. perforatum does not seem to be toxic to the mother nor to interfere with the progress of gestation during organogenesis.     

Hypericum perforatum Reduces Paracetamol‐Induced Hepatotoxicity and Lethality in Mice by Modulating Inflammation and Oxidative Stress

Hypericum perforatum is a medicinal plant with anti‐inflammatory and antioxidant properties, which is commercially available for therapeutic use in Brazil. Herein the effect of H. perforatum extract on paracetamol (acetaminophen)‐induced hepatotoxicity, lethality, inflammation, and oxidative stress in male swiss mice were investigated. HPLC analysis demonstrated the presence of rutin, quercetin, hypericin, pseudohypericin, and hyperforin in H. perforatum extract. Paracetamol (0.15–3.0 g/kg, p.o.) induced dose‐dependent mortality. The sub‐maximal lethal dose of paracetamol (1.5 g/kg, p.o.) was chosen for the experiments in the study. H. perforatum (30–300 mg/kg, i.p.) dose‐dependently reduced paracetamol‐induced lethality. Paracetamol‐induced increase in plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) concentrations, and hepatic myeloperoxidase activity, IL‐1β, TNF‐α, and IFN‐γ concentrations as well as decreased reduced glutathione (GSH) concentrations and capacity to reduce 2,2′‐azinobis‐(3‐ethylbenzothiazoline‐6‐sulfonate radical cation; ABTS˙⁺) were inhibited by H. perforatum (300 mg/kg, i.p.) treatment. Therefore, H. perforatum protects mice against paracetamol‐induced lethality and liver damage. This effect seems to be related to the reduction of paracetamol‐induced cytokine production, neutrophil recruitment, and oxidative stress. Copyright © 2015 John Wiley & Sons, Ltd.     

贯叶连翘中金丝桃素的提取及含量测定

目的:优选贯叶连翘中金丝桃素的提取工艺,并建立其含量测定方法。方法:考察高效液相色谱法测定金丝桃素含量的条件;以金丝桃素含量为指标,考察提取溶剂、提取时间、液料比、溶剂浓度等因素对超声提取金丝桃素的影响,筛选出最佳工艺。结果:确定最佳工艺为液料比:150∶1、溶剂:100%甲醇、提取时间30min;金丝桃素在0.0104～0.208μg(r2=0.9999)线性关系良好,平均回收率98.8%,RSD=0.54%。HPLC的色谱条件为:色谱柱:依利特HypersilODS2(5.0mm×200mm,5μ);流动相:甲醇-0.1mol.L-1 NaH2PO4(97∶3);柱温:30℃;检测器:SPD-20A UV/VIS;检测波长:590nm。结论本研究建立的方法稳定、准确、可行,可用于今后金丝桃素的研究。     

贯叶金丝桃的细胞培养

从贯叶金丝桃叶的嫩茎诱导愈伤组织的过程,探讨了悬浮培养和平板培养的方法,以及细胞悬浮培养时间、培养方法和接种密集度对贯叶金丝桃单细胞平板培养植板率的影响。在所实验的ＭＳ、Ｂ５、Ｗｈｉｔｅ３种培养基中,以ＭＳ培养基较好,其中ＭＳ＋２,４－Ｄ１ｍｇ／Ｌ＋ＮＡＡ １ ｍｇ／ｌ培养基愈伤组织诱导率为９２．８６％。细胞悬浮培养以Ｂ５培养基较好。普通单细胞平板培养法培养贯叶金丝桃单细胞的植板率很低,而以悬浮     

贯叶连翘挥发性成分分析

目的：测定贯叶连翘叶挥发油成分。方法：用连续蒸馏萃取挥发油提取仪提取（水蒸汽蒸馏,乙酸乙酯萃取）,然后采用ＧＣ－ＭＳ分析,结合计算机检索技术对其挥发油化学成分进行分离鉴定,应用色谱峰面积归一法测定各成分的相对百分含量。结果：分离、鉴定了５４种成分,占挥发油总量的９２．３２％。结论：化学成分多为倍半萜类物质,与国外报道贯叶连翘叶挥发油的成分相差很大。     

Sedative activity in mice of a hydroalcohol extract of Hypericum perforatum L.

The sedative activity of a hydroalcohol extract of Hypericum perforatum L. (Hypericaceae) and some of its isolated constituents was evaluated in mice. A dry hydroalcohol extract, orally administered at 26.5 mg/kg, induced a marked sedation as measured by two different tests (actimeter and sleep potentiation), compared with controls (diazepam, 2 mg/kg, per os). Several fractions were isolated from this hydroalcohol extract: hypericin, flavones, butanol fraction, aqueous fraction; a separation and purification method was developed for hypericin. None of the isolated fractions exhibited the same sedative activity as the hydroalcohol extract. © 1997 John Wiley & Sons, Ltd.     

光、赤霉素和乙烯利对贯叶连翘种子萌发的影响

目的 研究光，赤霉素和乙烯利对贯叶连翘Hypericum perforatum种子萌发的影响，为贯叶连翘的人工栽培提供理论依据。方法 直接发芽法，结果 贯叶连翘种子光下萌发率达79%，而暗中不萌发，赤霉素和乙烯利不仅能促进种子光下萌发，而且能诱导种子暗萌发，但乙烯利处理对幼苗胚根生长有明显的抑制作用。结论 贯叶连翘种子为需光种子，其需光性与赤霉素和乙烯利有关。     

贯叶金丝桃不同器官的分泌细胞团分布密度与金丝桃素含量的相关性研究

目的 研究贯叶金丝桃不同器官的分泌细胞团分布密度和金丝桃素含量及其相关性，为选择药用成分高的品种和合理的采收部位提供依据。方法 在显微镜下观察透明装片，统计叶、花萼和花瓣中分泌细胞团的分布密度，用HPLC法分析各器官中金丝桃素含量。结果 根、茎、果实和叶的中部不含金丝桃素类物质，叶缘部分含金丝桃素0.1456％，花萼含0.0653％，花瓣含1．2682％。结论 具有分泌细胞团的器官或部位含有金丝桃素类物质，且与其分布密度成正相关；而不具分泌细胞团的器官或部位，则不含有金丝桃素类物质。     

贯叶连翘提取物中金丝桃苷的分析鉴别与稳定性研究

目的探讨贯叶连翘提取物中金丝桃苷鉴别方法和稳定性。方法对贯叶连翘提取物进行化学、薄层、紫外鉴别。通过加速实验从不同影响因素分析贯叶连翘提取物中金丝桃苷的稳定性。结果贯叶连翘提取物的盐酸-镁粉反应显阳性,薄层色谱斑点清晰,紫外光谱在363 nm处有最大吸收峰。贯叶连翘提取物在酸、碱、热、湿、光、氧化、加速实验等条件下金丝桃苷的含量均有不同程度的损失。结论3种鉴别方法能很好地鉴别出黄酮苷类(金丝桃苷)的存在,定性方法简便、准确、专属性强。鉴于金丝桃苷的稳定性,在对贯叶连翘进行贮存时,应密闭、闭光、置阴凉干燥处保存。     

Effect of Hypericum perforatum L. Extract on Insulin Resistance and Lipid Metabolic Disorder in High‐Fat‐Diet Induced Obese Mice

Natural product Hypericum perforatum L. has been used in folk medicine to improve mental performance. However, the effect of H. perforatum L. on metabolism is still unknown. In order to test whether H. perforatum L. extract (EHP) has an effect on metabolic syndrome, we treated diet induced obese (DIO) C57BL/6J mice with the extract. The chemical characters of EHP were investigated with thin‐layer chromatography, ultraviolet, high‐performance liquid chromatography (HPLC), and HPLC‐mass spectrometry fingerprint analysis. Oral glucose tolerance test (OGTT), insulin tolerance test (ITT), and the glucose infusion rate (GIR) in hyperinsulinemic–euglycemic clamp test were performed to evaluate the glucose metabolism and insulin sensitivity. Skeletal muscle was examined for lipid metabolism. The results suggest that EHP can significantly improve the glucose and lipid metabolism in DIO mice. In vitro, EHP inhibited the catalytic activity of recombinant human protein tyrosine phosphatase 1B (PTP1B) and reduced the protein and mRNA levels of PTP1B in the skeletal muscle. Moreover, expressions of genes related to fatty acid uptake and oxidation were changed by EHP in the skeletal muscle. These results suggest that EHP may improve insulin resistance and lipid metabolism in DIO mice. © 2014 The Authors. Phytotherapy Research published by John Wiley & Sons Ltd.     

Effects of St John's wort (Hypericum perforatum L.) extract on plasma androgen concentrations in healthy men and women: a pilot study

St John's wort extract (SJW; Hypericum perforatum L.) is taken extensively as a putative herbal antidepressant. It has been shown to induce the activity of cytochrome P-450 3A4 (CYP3A4) and to increase the clearance of numerous drugs and steroids such as cortisol and ethinyl estradiol. This study was conducted to determine if SJW exposure also alters the concentrations of circulating androgenic steroid hormones. The study was conducted using healthy volunteers (6M, 6F) studied before and after a 14-day treatment period with a SJW preparation previously demonstrated to induce the activity of CYP3A4. Plasma concentrations of testosterone, dihydrotestosterone (DHT), dehydroepiandrosterone sulfate (DHEAS), sex hormone-binding globulin (SHBG) and the combined concentrations of androsterone sulfate (AoS) and epiandrosterone sulfate (epiAoS) were measured by immunoassay methods. The results of analysis demonstrated that SJW did not significantly alter the majority of the androgens studied (p > 0.05) although the combined concentrations of the 5alpha-reduced steroids, AoS and epiAoS, significantly declined following treatment in all subjects (p = 0.02), and in males (p = 0.04). Furthermore, the testosterone to DHT ratio was increased in both men and women. Although the latter increase did not reach statistical significance, it is also consistent with the possible inhibition of 5alpha-reductase by SJW. It is concluded that despite significant induction of CYP3A4, short term administration of SJW does not significantly alter the concentrations of most circulating androgens in men and women but may produce a dimunition in some of the circulating 5alpha-reduced androgens.     

越鞠胶囊与贯叶连翘抗抑郁作用的对比研究

目的:研究越鞠胶囊、贯叶连翘的抗抑郁作用。方法:采用小鼠悬尾实验、小鼠强迫游泳实验、利血平诱导小鼠体温下降实验和开场实验研究越鞠胶囊、贯叶连翘的抗抑郁作用。结果:在小鼠悬尾实验中,越鞠胶囊大剂量组、贯叶连翘大、中剂量组能明显缩短模型小鼠的不动时间;在小鼠强迫游泳实验中,越鞠胶囊、贯叶连翘各剂量组能明显缩短模型小鼠的不动时间;在利血平诱导的体温下降实验中,越鞠胶囊大剂量组、贯叶连翘大、中剂量组能明显抑制小鼠体温的下降;实验表明贯叶连翘提取物的作用效价强度要大于越鞠胶囊。结论:越鞠胶囊、贯叶连翘有一定的的抗抑郁作用,贯叶连翘提取物的作用效价强度要大于越鞠胶囊。     

贯叶连翘活性成分研究新进展

介绍了贯叶连翘的活性成分,抗抑郁、抗肿瘤、抗病毒等药理作用和副作用的研究新进展。     

HPLC法测定不同干燥方法的贯叶连翘中黄酮类化合物的含量

目的研究HPLC测定条件,并测定不同干燥方法贯叶连翘中黄酮类化合物芦丁、金丝桃苷的含量.方法色谱柱DiscoveryC18(5μm,25cm×4.6mm);流动相水-乙腈-磷酸(825∶175∶1);流速1.0mL/min;检测波长365nm.结果60℃烘干4h贯叶连翘中芦丁和金丝桃苷的含量最高,总含量可达到1.677%.芦丁的线性范围为0.107～2.675μg,平均回收率为99.32%,RSD=1.007%,金丝桃苷的线性范围为0.107～2.675μg,平均回收率为99.54%,RSD=3.591%.结论该方法分析简便、灵敏、迅速、准确.     

Antiviral In vitro activity of Hypericum perforatum l. extract on the human cytomegalovirus (HCMV)

The total methanol extract and chromatographic fractions of the extract of the aerial parts of Hypericum perforatum L. were investigated for their antiviral activity against human cytomegalovirus (HCMV) AD-169 laboratory strain by modification of the widely used diagnostic method ‘shell-vial’ culture. The plant selection was based on its traditional use against viruses of the Herpes family in Greece. The results showed extract fraction III had the most potent antiviral activity against HCMV which was also superior to that of ganciclovir. Copyright © 1998 John Wiley & Sons, Ltd.