骨松汤对去卵巢小鼠骨质疏松影响的实验研究

目的:建立去卵巢小鼠骨质疏松模型,观察骨松汤对去卵巢小鼠骨质疏松骨代谢指标及骨密度的影响,并探讨骨松汤对去卵巢骨质疏松影响的机制。方法:将40只雌性小鼠随机分为空白组、模型组、骨松汤组、钙尔奇组,每组各10只。除空白组外,其余各组经双侧卵巢切除术建立骨质疏松小鼠模型。骨松汤组在造模后灌胃骨松汤水煎剂每天早晚各3 m L(1.72 g/10 g),钙尔奇组给予钙尔奇灌胃每天早晚各3 m L(0.42 g/10 g)、空白组和模型组灌胃等量生理盐水,共12 w。12 w后测各组定小鼠血清碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)、骨钙素(BGP)和雌二醇(E2)及骨密度。结果:1.血清碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)、骨钙素(BGP)和血清雌二醇(E2):骨松汤组治疗前后比较血清碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)、骨钙素(BGP)明显降低,雌二醇(E2)明显升高,P0.05,差异显著,有统计学意义。钙尔奇组治疗前后比较的血清碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)、骨钙素(BGP)明显降低,雌二醇(E2)明显升高,P0.05,差异显著,有统计学意义。治疗后骨松汤组和钙尔奇组2组比较,血清碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)、骨钙素(BGP)明显降低,雌二醇(E2)明显升高,P0.05,差异显著,有统计学意义。骨松汤可以升高去卵巢小鼠骨质疏松的血清雌二醇(E2),降低血清碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)、骨钙素(BGP),改善去卵巢骨质疏松小鼠骨密度。2.骨密度:治疗后骨松汤组和钙尔奇组的骨密度与空白组接近,与模型组相比差异显著,有统计学意义。2组组间比较差异显著,P0.05。结论:骨松汤可以改善去卵巢小鼠骨密度,其机制与其减缓雌激素水平有关。     

福善美合仙灵骨葆治疗绝经后骨质疏松症骨钙素和降钙素及骨密度的临床研究

目的 研究福善美合仙灵骨葆治疗绝经后骨质疏松症骨钙素、降钙素及骨密度水平的变化.方法 56例绝经后骨质疏松症随机分治疗组(33例)和对照组(23例),治疗组口服福善美、仙灵骨葆和钙片,对照组口服钙片,均经6个月治疗,观察两组治疗前后骨钙素、降钙素和骨密度值,并进行统计学分析.结果 治疗组和对照组治疗前后的骨密度T分数分别为:-3.62±0.74、-2.13±0.61和-3.49±0.77、-3.20±0.68,骨钙素分别为:48.23±7.52、29.61±10.42和47.21±11.73、45.31±11.25,降钙素分别为43.14±8.69、52.93±10.75和42.71±5.67,43.21±7.52.比较治疗组治疗前后骨密度T值、骨钙素和降钙素水平均有显著差异(P＜0.05),而对照组均无显著差异(P＞0.05),两组治疗后的骨密度T值、骨钙素和降钙索比较均有显著差异(P＜0.05).结论 福善美合仙灵骨葆治疗绝经后骨质疏松症使降钙素的水平明显升高,骨钙素水平明显降低,对绝经后骨质疏松症有明显的疗效.     

福善美合仙灵骨葆治疗绝经后骨质疏松症骨钙素和降钙素及骨密度的临床研究

目的 研究福善美合仙灵骨葆治疗绝经后骨质疏松症骨钙素、降钙素及骨密度水平的变化.方法 56例绝经后骨质疏松症随机分治疗组(33例)和对照组(23例),治疗组口服福善美、仙灵骨葆和钙片,对照组口服钙片,均经6个月治疗,观察两组治疗前后骨钙素、降钙素和骨密度值,并进行统计学分析.结果 治疗组和对照组治疗前后的骨密度T分数分别为:-3.62±0.74、-2.13±0.61和-3.49±0.77、-3.20±0.68,骨钙素分别为:48.23±7.52、29.61±10.42和47.21±11.73、45.31±11.25,降钙素分别为43.14±8.69、52.93±10.75和42.71±5.67,43.21±7.52.比较治疗组治疗前后骨密度T值、骨钙素和降钙素水平均有显著差异(P＜0.05),而对照组均无显著差异(P＞0.05),两组治疗后的骨密度T值、骨钙素和降钙索比较均有显著差异(P＜0.05).结论 福善美合仙灵骨葆治疗绝经后骨质疏松症使降钙素的水平明显升高,骨钙素水平明显降低,对绝经后骨质疏松症有明显的疗效.     

补肾健脾方联合钙尔奇D对绝经后乳腺癌合并骨质疏松患者骨密度和骨代谢的影响

目的探讨补肾健脾方联合钙尔奇D对绝经后乳腺癌合并骨质疏松患者骨密度和骨代谢的影响。方法自2015年1月—2016年1月,前瞻性收集我院收治的绝经后乳腺癌合并骨质疏松患者100例,将患者随机分为治疗组和对照组,每组各50例。治疗组给予补肾健脾方联合钙尔奇D治疗,对照组仅给予钙尔奇D治疗。观察两组患者股骨颈骨密度、降钙素、碱性磷酸酶、骨钙素、抗酒石酸酸性磷酸酶5b、转化生长因子-β1和Ⅰ型胶原羧基末端肽治疗前后变化情况。结果两组患者治疗前骨密度水平差异无统计学意义(P=0.729)。与对照组比较,治疗3个月和6个月后治疗组骨密度显著增加(P=0.016)。两组患者治疗前、后碱性磷酸酶、转化生长因子-β1和Ⅰ型胶原羧基末端肽等差异均无统计学意义(P0.05)。两组患者治疗前降钙素、骨钙素和抗酒石酸酸性磷酸酶5b等差异无统计学意义(P0.05)。但与对照组比较,治疗后6个月时治疗组患者降钙素显著增加(P=0.005);骨钙素显著增加(P=0.000);抗酒石酸酸性磷酸酶5b显著降低(P=0.000)。结论与对照组比较,补肾健脾方联合钙尔奇D有助于改善绝经后乳腺癌合并骨质疏松患者骨密度和骨代谢相关指标。     

壮骨胶囊治疗Ⅰ型骨质疏松症93例生化指标观察

９３ 例绝经后妇女骨质疏松症经用壮骨胶囊（ 益肾健脾） 治疗后症状、体征显著改善，优良率达９３．５ ％ ，骨密度明显升高（Ｐ＜０ ．０１） ，血清钙、磷、骨钙素（ＢＧＰ） 治疗前后无明显差异，而血清碱性磷酸酶（ＡＬＰ） 显著降低，血清降钙素（ＣＴ）明显上升（Ｐ皆＜０ ．０１） ，说明壮骨胶囊治疗Ⅰ型骨质疏松症有效，在生化指标上起到调节作用，显著优于采用钙尔奇Ｄ治疗的对照组。     

抗骨松液对骨质疏松大鼠治疗作用的研究

目的:探讨抗骨松液对绝经后骨质疏松症的治疗作用。方法:用去大鼠双侧卵巢复制绝经后骨质疏松症的模型,观察抗骨松液对其股骨骨密度、骨钙素、血清雌二醇的影响。结果:抗骨松液可提高雌二醇、骨钙素水平,显著增加骨密度。结论:抗骨松液对绝经后骨质疏松症有良好的治疗作用。     

福善美合仙灵骨葆治疗绝经后骨质疏松症骨钙素和降钙素及骨密度的临床研究

目的 研究福善美合仙灵骨葆治疗绝经后骨质疏松症骨钙素、降钙素及骨密度水平的变化.方法 56例绝经后骨质疏松症随机分治疗组(33例)和对照组(23例),治疗组口服福善美、仙灵骨葆和钙片,对照组口服钙片,均经6个月治疗.观察两组治疗前后骨钙素、降钙素和骨密度值,并进行统计学分析.结果 治疗组和对照组治疗前后的骨密度丁分数分别为:-3,62±0.74、-2.13±.61和-3.49±,77、-3.20±0.68,骨钙素分别为:48.23±7,52、29,61±10.42和47.21±11,73、45.31±11.25,降钙素分别为43.14±8.69、52,93±10.75和42.71±5,67、43.21±7、52.比较治疗组治疗前后骨密度T值、骨钙素和降钙素水平均有显著差异(P<0.05),而对照组均无显著差异(P>0.05),两组治疗后的骨密度T值、骨钙素和降钙素比较均有显著差异(P<0.05).结论 福善美合仙灵骨葆治疗绝经后骨质疏松症使降钙素的水平明显升高,骨钙素水平明显降低,对绝经后骨质疏松症有明显的疗效.     

抗骨松液对骨质疏松大鼠治疗作用的研究

目的：探讨抗骨松液对绝经后骨质疏松症的治疗作用。方法：用去大鼠双侧卵巢复制绝经后骨质疏松症的模型，观察抗骨松液对其股骨骨密度、骨钙素、血清雌二醇的影响。结果：抗骨松液可提高雌二醇、骨钙素水平，显著增加骨密度。结论：抗骨松液对绝经后骨质疏松症有良好的治疗作用。     

中药治疗绝经后骨质疏松症的疗效观察及其机制探讨

目的观察中药治疗绝经后骨质疏松症的临床疗效并探讨其机制。方法25例患者用中药治疗6个月,于治疗前后分别测定患者腰椎(L2-4),股骨颈和Wards三角骨密度和血清中雌二醇、睾酮、甲状旁腺素、降钙素和骨钙素。结果治疗6个月后发现,患者骨密度分别增加2．07％,1．68％和2．12％。血清中雌二醇升高(P＜0．05)。结论补肾中药可以治疗绝经后骨质疏松症,其部分机制是中药促进雌二醇的分泌而降低骨转换率。     

强骨丹对去卵巢大鼠骨质疏松症防治作用的实验研究

[目的]探讨中药强骨丹对绝经后骨质疏松症的防治作用。[方法]运用切除大鼠卵巢方法建立去卵巢骨质疏松症模型,随机分为模型组、强骨丹高低剂量组、西药组,同时设假手术对照组。采用双能X线骨密度测定法、放射免疫分析法和ELISA法等检测各组实验鼠骨密度 (BMD)、骨矿含量 (BMC)、血清雌二醇 (E₂)、骨钙素 (BGP)、抗酒石酸酸性磷酸酶5b(TRACP5b)以及尿脱氧吡啶啉 (Dpol)。[结果]强骨丹可明显升高BMD、BMC,提高血清E₂ 水平,降低TRACP5b和Dpd水平,并降低BGP含量。[结论]强骨丹通过调节机体相关内分泌功能,抑制骨吸收,降低骨的转换率,减少骨丢失,增加骨密度,对绝经后骨质疏松症有防治作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.