CpG寡脱氧核苷酸对外周血单个核细胞功能的影响：1型糖尿病患者与健康者对照

背景：有研究表明CpG寡脱氧核苷酸可增强外周血单个核细胞的功能,但对1型糖尿病患者的影响至今少有报道。目的：观察CpG寡脱氧核苷酸对1型糖尿患者患者与健康志愿者外周血单个核细胞γ干扰素、白细胞介素12,10表达的影响。方法：将1型糖尿病患者与健康志愿者的外周血单个核细胞根据刺激物不同分为空白对照组、CpG寡脱氧核苷酸组。用RT-PCR法检测外周血单个核细胞γ干扰素、白细胞介素12mRNA和白细胞介素10mRNA的表达。结果与结论：1型糖尿患者γ干扰素和白细胞介素12mRNA的表达明显低于健康志愿者（P〈0.01）。1型糖尿患者与健康志愿者外周血单个核细胞经CpG寡脱氧核苷酸组刺激后,γ干扰素和白细胞介素12mRNA的表达增高（P〈0.01）,白细胞介素10mRNA的表达无差异（P〉0.05）。结果提示,CpG寡脱氧核苷酸可促进1型糖尿患者外周血单个核细胞表达γ干扰素和白细胞介素12。     

CpG ODN对2型糖尿病患者外周血单个核细胞功能的影响

近年发现,有免疫刺激活性的细菌DNA和一些人工合成的寡脱氧核苷酸序列中都含有非甲基化5‘-CG-3‘二核苷酸,统称为CpG寡脱氧核苷酸(CpG ODN)[1], 主要免疫效应为刺激单核-巨噬细胞产生细胞因子IL-12、IL-18和TNF-α,促进B细胞分泌IL-12和IL-6;通过IL-12上调NK细胞产生γ-干扰素(IFN-γ), 增强杀伤活性;通过上述一系列细胞因子和炎症细胞的相互作用,增强机体免疫能力[2].但有关CpG ODN能否改善糖尿病患者外周血单个核细胞功能的研究较少.……     

CpG ODN对2型糖尿病患者外周血单个核细胞功能的影响

近年发现，有免疫刺激活性的细菌DNA和一些人工合成的寡脱氧核苷酸序列中都含有非甲基化5＇-CG-3’二核苷酸，统称为CpG寡脱氧核苷酸（CpGODN），主要免疫效应为刺激单核-巨噬细胞产生细胞因子IL-12、IL-18和TNF-α，促进B细胞分泌IL-12和IL-6；通过IL-12上调NK细胞产生了γ-干扰素（IFN-γ），增强杀伤活性；通过上述一系列细胞因子和炎症细胞的相互作用，增强机体免疫能力。但有关CpG ODN能否改善糖尿病患者外周血单个核细胞功能的研究较少。     

食管鳞状细胞癌患者血清T辅助细胞类细胞因子的表达及其临床意义

目的 探讨食管癌患者血清T辅助细胞(Th1、Th2)类细胞因子的表达及其临床意义.方法 采用双抗体酶联免疫吸附法(ELISA)检测45例食管鳞状细胞癌患者及30名健康体检者外周血Th1[干扰素(INF)-γ、白细胞介素(IL)-2]、Th2类(IL-4)细胞因子的含量.结果 食管癌患者Th1类细胞因子INF-γ、IL-2表达含量较健康对照组降低[(11.51±2.15)、(16.31±2.27)ng/L,t=18.24,P＜0.01);(10.20±2.10)、(16.92±2.41)ng/L,t=15.31,P＜0.05];Th2类细胞因子(IL-4)较健康对照组升高[(5.30 -0.11)、(2.23±0.29)ng/L,t=5.79,P＜0.05],且其表达趋势与肿瘤的TNM分期有关.结论 食管癌患者体内存在Th1/Th2漂移现象.该现象可能与肿瘤细胞发生免疫逃逸有关.

Abstract：

Objective To study the expression of Th1 and Th2 types cytokines in patients with esophageal squamous cell carcinoma and the clinical significance. Methods The expressions of Th1 ( INF-γ、IL-2) and Th2 (IL-4) types cytokines in cultured peripheral blood mononuclear cells (PBMC) from 30 healthy controls and 45 esophageal squamous cell carcinoma patients were determined by Enzyme-linked immunosorbent assay (ELISA). Results Compared with the control group, the expressions of Th1 type cytokines, IL-2 and INF-γwere significantly lower in the cultured PBMCs from the cancer patients (tINF-γ= 18. 24 ,tIL-2 = 15.31 ,Ps ＜0. 01 ) ,while the expression of Th2 type cytokines,IL-4 was significantly higher in the cultured PBMCs from the cancer patients ( tIL-4 = 5.79, P ＜ 0. 05 ). The expression changes of the cytokines showed a correlation with TNM (tumou-node-metastasis) stage of the tumor. Conclusion The Th1/Th2 shift existed in patients with esophageal squamous cell carcinoma and it might be one of the underline mechanisms of tumor immune escape.     

p38MAPK和COX-2mRNA在创伤后多器官功能障碍综合征患者外周血中的表达

目的 探讨创伤后多功能障碍综合征(multiple organ dysfunction syndrome,MODS)患者外周血单核细胞(peripheral blood mononuclear cells,PBMCs)环氧化酶-2(cyclooxygenase-2,COX-2)与p38丝裂原活化蛋白激酶(p38 mitogen-activated protein kinase,p38MAPK)mRNA的表达以及与MODS病情变化的关系.方法 选择符合1995年中华医学会急诊分会危重病专业组制定的MODS病情分期诊断标准和1991年Knaus提出急性生理和慢性健康评分Ⅲ(APACHE Ⅲ)的患者40例,其中男22例,女18例;与患者年龄、性别相匹配的健康对照组40名.用酶联免疫吸附试验法(ELISA)检测PBMCs培养上清液COX-2与p38MAPK含量;用逆转录-聚合酶链反应(RT-PCR)法检测PBMCs中COX-2与p38MAPK的mRNA表达.并分析它们的表达与APACHEⅢ评分的相关性.结果 MODS组患者PBMCs的COX-2与p38MAPK含量及两者的mRNA表达均显著高于健康对照组(均P<0.05);MODS死亡患者PBMCs的COX-2与p38MAPK含量及两者的mRNA表达与存活患者比较,差异具有统计学意义(均P<0.05);相关分析显示,PBMCs培养上清液中COX-2与p38MAPK含量呈正相关(r=0.614 7,P<0.01);患者 PBMCs的COX-2和p38MAPK mRNA表达与APACHEⅢ评分呈正相关(r1=0.500 9,P1<0.05,r2=0.531 6,P2<0.05).结论 COX-2与p38MAPK参与了MODS的发病过程,而且可作为判断MODS预后的辅助指标.

Abstract：

Objective To explore the expression of COX-2 and p38MAPK in patients with trauma MODS. Methods Forty MODS patients were evaluated. The levels of peripheral blood mononuclear cells COX-2 and p38MAPK in MODS patients and 40 normal controls was detected by enzyme linked immunosor-bent assay (ELISA). RT-PCR was used to measure the COX-2 mRNA and p38MAPK mRNA expression of in PBMCs. ANOV and correlation analysis were used in statistical analysis. Results The levels of COX-2 and p38MAPK of PBMCs and the mRNA expression in MODS group were higher than in control group (all P <0.05). The levels of COX-2 and p38MAPK of PBMCs and the mRNA expression in dead group were higher than in survival group( all P <0.05). The levels of COX-2 and p38MAPK of PBMCs were positively correlated, (r =0.6 147, P<0.01). The expression of COX-2 mRNA, p38MAPK mRNA of PBMCs and APACHE I scoring were positively correlated (r1 =0.5 009, P1 <0.05,r2 =0. 5 316, P2 <0. 05). Conclusions COX-2 and p38MAPK of PBMCs take part in the onset of MODS, and may service as index to judge the prognosis of MODS.     

降钙素基因相关肽、内皮素与2型糖尿病肾病的相关性研究

Objective To investigate the tendency of plasma levels of calcitonin gene-related peptide(CGRP)and endothelin(ET)in type 2 diabetic nephropathy,and explore the relationship between calcitonin gene-related peptide,endothelin and pathogenesis of diabetic nephropathy.Methods A total of 25 healthy controls(healthy control group)and 83 patients with type 2 diabetic mellitus(T2DM group)were enrolled in the investigation.According to level s of urinary albumin excretion rate(UAER),the 83 T2DM patients group were further divided into three subgroups:normal UAER subgroup(n=28),microalbuminuira subgroup(n=29)and clinical proteinuria subgroup(n=26).Plasma contents of calcitonin gene-related peptide and endothelin were measured with radioimmunoassay(RIA).Results The level of ET was significantly higher in T2DM group than that in healthy control group(P＜0.01),positively correlative tO UAER(r=0.65,P＜0.01).The plasma level of CGRP was significantly lower in the patients with diabetic nephropathy than the healthy controls,negatively correlative tO UAER(r=-0.71,P＜0.01).There was negative correlation between plasma levels of ET and CGRP(r=-0.62,P＜0.01).Conclusion The disequilibrium of CGRP and ET in plasma might play an important role in the process of diabetic nephropathy.Therefore,keeping balance of levels of CGRP and ET in plasma contributes to prevention and cure of diabetic nephropathy.     

降钙素基因相关肽、内皮素与2型糖尿病肾病的相关性研究

Objective To investigate the tendency of plasma levels of calcitonin gene-related peptide(CGRP)and endothelin(ET)in type 2 diabetic nephropathy,and explore the relationship between calcitonin gene-related peptide,endothelin and pathogenesis of diabetic nephropathy.Methods A total of 25 healthy controls(healthy control group)and 83 patients with type 2 diabetic mellitus(T2DM group)were enrolled in the investigation.According to level s of urinary albumin excretion rate(UAER),the 83 T2DM patients group were further divided into three subgroups:normal UAER subgroup(n=28),microalbuminuira subgroup(n=29)and clinical proteinuria subgroup(n=26).Plasma contents of calcitonin gene-related peptide and endothelin were measured with radioimmunoassay(RIA).Results The level of ET was significantly higher in T2DM group than that in healthy control group(P＜0.01),positively correlative tO UAER(r=0.65,P＜0.01).The plasma level of CGRP was significantly lower in the patients with diabetic nephropathy than the healthy controls,negatively correlative tO UAER(r=-0.71,P＜0.01).There was negative correlation between plasma levels of ET and CGRP(r=-0.62,P＜0.01).Conclusion The disequilibrium of CGRP and ET in plasma might play an important role in the process of diabetic nephropathy.Therefore,keeping balance of levels of CGRP and ET in plasma contributes to prevention and cure of diabetic nephropathy.     

降钙素基因相关肽、内皮素与2型糖尿病肾病的相关性研究

Objective To investigate the tendency of plasma levels of calcitonin gene-related peptide(CGRP)and endothelin(ET)in type 2 diabetic nephropathy,and explore the relationship between calcitonin gene-related peptide,endothelin and pathogenesis of diabetic nephropathy.Methods A total of 25 healthy controls(healthy control group)and 83 patients with type 2 diabetic mellitus(T2DM group)were enrolled in the investigation.According to level s of urinary albumin excretion rate(UAER),the 83 T2DM patients group were further divided into three subgroups:normal UAER subgroup(n=28),microalbuminuira subgroup(n=29)and clinical proteinuria subgroup(n=26).Plasma contents of calcitonin gene-related peptide and endothelin were measured with radioimmunoassay(RIA).Results The level of ET was significantly higher in T2DM group than that in healthy control group(P＜0.01),positively correlative tO UAER(r=0.65,P＜0.01).The plasma level of CGRP was significantly lower in the patients with diabetic nephropathy than the healthy controls,negatively correlative tO UAER(r=-0.71,P＜0.01).There was negative correlation between plasma levels of ET and CGRP(r=-0.62,P＜0.01).Conclusion The disequilibrium of CGRP and ET in plasma might play an important role in the process of diabetic nephropathy.Therefore,keeping balance of levels of CGRP and ET in plasma contributes to prevention and cure of diabetic nephropathy.     

降钙素基因相关肽、内皮素与2型糖尿病肾病的相关性研究

Objective To investigate the tendency of plasma levels of calcitonin gene-related peptide(CGRP)and endothelin(ET)in type 2 diabetic nephropathy,and explore the relationship between calcitonin gene-related peptide,endothelin and pathogenesis of diabetic nephropathy.Methods A total of 25 healthy controls(healthy control group)and 83 patients with type 2 diabetic mellitus(T2DM group)were enrolled in the investigation.According to level s of urinary albumin excretion rate(UAER),the 83 T2DM patients group were further divided into three subgroups:normal UAER subgroup(n=28),microalbuminuira subgroup(n=29)and clinical proteinuria subgroup(n=26).Plasma contents of calcitonin gene-related peptide and endothelin were measured with radioimmunoassay(RIA).Results The level of ET was significantly higher in T2DM group than that in healthy control group(P＜0.01),positively correlative tO UAER(r=0.65,P＜0.01).The plasma level of CGRP was significantly lower in the patients with diabetic nephropathy than the healthy controls,negatively correlative tO UAER(r=-0.71,P＜0.01).There was negative correlation between plasma levels of ET and CGRP(r=-0.62,P＜0.01).Conclusion The disequilibrium of CGRP and ET in plasma might play an important role in the process of diabetic nephropathy.Therefore,keeping balance of levels of CGRP and ET in plasma contributes to prevention and cure of diabetic nephropathy.     

降钙素基因相关肽、内皮素与2型糖尿病肾病的相关性研究

Objective To investigate the tendency of plasma levels of calcitonin gene-related peptide(CGRP)and endothelin(ET)in type 2 diabetic nephropathy,and explore the relationship between calcitonin gene-related peptide,endothelin and pathogenesis of diabetic nephropathy.Methods A total of 25 healthy controls(healthy control group)and 83 patients with type 2 diabetic mellitus(T2DM group)were enrolled in the investigation.According to level s of urinary albumin excretion rate(UAER),the 83 T2DM patients group were further divided into three subgroups:normal UAER subgroup(n=28),microalbuminuira subgroup(n=29)and clinical proteinuria subgroup(n=26).Plasma contents of calcitonin gene-related peptide and endothelin were measured with radioimmunoassay(RIA).Results The level of ET was significantly higher in T2DM group than that in healthy control group(P＜0.01),positively correlative tO UAER(r=0.65,P＜0.01).The plasma level of CGRP was significantly lower in the patients with diabetic nephropathy than the healthy controls,negatively correlative tO UAER(r=-0.71,P＜0.01).There was negative correlation between plasma levels of ET and CGRP(r=-0.62,P＜0.01).Conclusion The disequilibrium of CGRP and ET in plasma might play an important role in the process of diabetic nephropathy.Therefore,keeping balance of levels of CGRP and ET in plasma contributes to prevention and cure of diabetic nephropathy.     

T细胞免疫球蛋白-黏蛋白-1对卵蛋白致敏小鼠气道MUC5AC及Th1/Th2细胞因子表达的影响

目的 研究T细胞免疫球蛋白-黏蛋白-1（TIM-1）对卵蛋白（OVA）致敏小鼠气道MUC5AC及Th1/Th2细胞因子表达的影响,探讨气道黏液高分泌的机制.方法 OVA腹腔注射致敏小鼠,随机分组为：正常对照组（A组）、哮喘组（B组）和哮喘＋ TIM-1抗体处理组（C组）,每组8只,酶联免疫吸附试验（ELISA）检测小鼠肺泡灌洗液（BALD中IL-4、IFN-γ的水平变化,实时RT-PCR检测外周血单个核细胞（PBMCs） TIM-1 mRNA及气道MUC5AC mRNA表达.结果 （1）与A组小鼠外周血PBMCs表达TIM-1 mRNA（0.618±0.043）相比,B组（1.129±1.101）及C组（0.898±0.071）TIM-1 mRNA表达明显增多（P＜0.01）,且C组明显低于B组（P＜0.01）;（2）B组和C组小鼠BALF中IL-4表达分别为（67.123±3.341） ng/L和（44.217±2.201）ng/L,较A组[（23.211±2.142） ng/L]明显增多（P＜0.01）,且C组明显低于B组（P＜0.01）;B组及C组小鼠BALF表达IFN1分别为（53.475±4.776） ng/L和（87.116±5.611）ng/L,均低于A组[（124.624±9.292） ng/L] （P ＜0.01）,而C组与B组相比,有所增加（P＜0.01）;（3）B组（1.004±0.081）及C组（0.673±0.029）小鼠气道MUC5A mRNA表达与A组（0.314±0.027）相比,明显增加（P＜0.01）,且C组低于B组（P＜0.01）;（4）小鼠外周血PBMCs TIM-1 mRNA表达与IL-4、MUC5AC表达呈正相关,而与IFN-γ表达呈负相关.结论 抑制TIM-1表达可减少IL-4和MUC5AC的分泌,提高IFN-γ表达,提示在调节黏液高分泌及哮喘治疗中具有意义.     

再生障碍性贫血患者外周血Th 1/Th2水平测定及其临床意义

目的：探讨辅助性T(T helper,Th)细胞亚群数量和功能的变化在再生障碍性贫血(再障)患者发病机制中的作用，及这两种变化之间的关系；为再障的诊断及疗效预测提供实验依据。方法：流式细胞术检测再障患者及正常人外周血T细胞亚群、Th1和Th2细胞；RT-PCR法测定再障患者及正常人外周血中Th1，Th2型细胞因子基因表达；用ELISA法测定再障患者血浆中IFN-γ和IL-4的水平。结果：再障患者Th1/Th2显著失衡，Th1细胞显著增多，Th1型细胞因子IFN-γmRNA的阳性表达率和血浆IFN-γ浓度均较正常对照显著升高；再障患者Th1细胞百分数分别与IFN-γmRNA相对表达强度(RV)和血浆IFN-γ浓度呈正相关；Th1细胞百分比、IFN-γmRNA的RV及血浆IFN-γ的浓度在比值倒置型与比值超高型患者体内相近且均高于比值正常型患者。结论：Th细胞亚群失衡，Th1细胞数量增多及功能亢进可能是再障发病的重要免疫机制之一；Th1细胞数量及功能异常相互作用是再障患者疾病发生和发展的根本原因；CD4+T或CD8+T细胞相对变化可能是Th细胞在发挥免疫抑制作用后的外在表象。     

地塞米松对系统性红斑狼疮患者外周血单个核细胞中细胞因子分泌及T细胞亚群表达的影响

目的 探讨地塞米松对系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMCs)中白细胞介素(IL)-17、干扰素(IFN)-γ分泌水平和Th17、Tc17、Th1、Tc1等T细胞亚群表达的影响.方法 SLE患者和健康对照者的PBMCs分空白孔、佛波酯(PMA)/离子霉素(Ionomycin)孔、PMA/Ionomycin+地塞米松(DEX)孔行体外培养,运用四色流式细胞术检测PBMCs表达Th17、Tc17、Th1、Tc1等T细胞亚群的百分比,采用酶联免疫吸附法测定SLE患者和健康对照者血浆及PBMCs培养上清中IL-17、IFN-γ的表达水平.结果 SLE组患者血浆IL-17[138.98(84.82～187.04)ng/L]、IFN-γ[21.92(15.95～27.09)ng/L]含量均高于正常对照组[57.21(47.78～72.12)ng/L,13.43(7.04～17.37)ng/L].无PMA刺激条件下,SLE患者PBMCs培养上清中细胞因子水平、PBMCs中各T细胞亚群的百分比与正常对照组相比差异均无统计学意义(P均＞0.05);加入PMA刺激后,SLE患者PBMCs上清中IL-17的水平[(26.43±10.04)ng/L]和外周血Th17[(2.49±1.49)%]、Tc1[(44.89±16.43)%]细胞的比例均显著高于正常对照组[(18.06±5.42)ng/L,(1.47±0.73)%,(31.41±9.05)%)(P均＜0.05),SLE患者Th1、Tc17细胞百分比与正常对照组相比,差异均无统计学意义(P均＞0.05);地塞米松能明显抑制活化状态下的PBMCs分泌IL-17的水平[(16.72±6.09)ng/L](P＜0.01),且显著下调Th17(1. 34±0.76)%、Tc1(34.62±17.25)%细胞百分比(P均＜0.05),而地塞米松对IL-17的抑制作用更强.结论 SLE患者体内T细胞亚群及其相应细胞因子的表达水平存在明显异常,地塞米松能干扰SLE患者体内细胞因子网络失衡的免疫病理过程,且对IL-17有明显的抑制作用,为临床使用糖皮质激素治疗SLE提供新的理论依据和实验室基础.     

结核病合并慢性乙肝患者TNF—α、IFN-γ、IL-12变化及意义

【目的】研究结核病合并慢性乙肝患者血清肿瘤坏死因子-α（TNF-α）、γ干扰素（IFN-γ）及白细胞介素-12（IL-12）水平的变化及意义。【方法】肺结核并HBsAg阳性患者21例（A组）,单纯肺结核患者30例（B组）,健康对照组30例（C组）。应用酶联免疫吸附法（ELISA）测定三组血清中TNF-α、IFN-γ、IL-12浓度水平。【结果】A组和B组TNF-α水平均高于C组（P〈0．01）;但A组T组和B组之间TNF-α水平差异无显著性（P〉0．05）;B组IFN-γ、IL-12水平均低于C组（P〈0．05）;而A组IFN-γ、IL-12水平均低于B组（P〈0．05）。【结论】在结核病合并慢性乙肝患者的体内,可能存在着显著的Th1细胞免疫应答减弱。     

重组鼠白细胞介素-12腺病毒载体对哮喘小鼠模型IL-4、IFN-γ表达的影响

目的探讨重组鼠白细胞介素-12(IL-12)基因的腺病毒载体(AdCMVIL-12)对小鼠支气管哮喘模型IL-4、IFN-γ表达的影响。方法雌性BALB/c小鼠50只,按数字随机表法分为5组,每组10只:哮喘模型组、AdCMVIL-12组、AdCMVLacZ组、激素治疗组和正常对照组。并于第20天分别鼻内吸入50μL生理盐水和5×108 PFU AdC-MVIL-12。第28天收集各组小鼠的支气管肺泡灌洗液(BALF),检测IL-4、IFN-γ的变化,RT-PCR方法检测各组肺组织中IL-4、IFN-γmRNA的表达水平。结果哮喘模型组与对照组、AdCMVIL-12组相比较,BALF中的IL-4表达水平明显升高,而IFN-γ在AdCMVIL-12组中的表达水平显著上升。RT-PCR结果显示,哮喘模型组小鼠肺组织IL-4mRNA的表达与AdCMVIL-12组、激素治疗组及正常对照组相比较明显升高;而IFN-γmRNA的表达水平明显下降。结论 AdCMVIL-12对哮喘小鼠的气道及肺组织内的IL-4表达有明显的抑制作用,其机制可能与AdCMVIL-12阻断IL-4的表达,调节Th1/Th2类细胞因子的平衡有关。     

IL-23在单纯疱疹病毒Ⅱ型致病中的作用

目的 探讨IL-23在单纯疱疹病毒Ⅱ型(HSV-Ⅱ)致病机制中的作用.方法 应用ELISA法检测HSV病毒感染复发患者外周血IL-23、IL-12、IFN-γ、IL-14、IL-10水平,以及细胞培养液中IFN-γ水平,流式细胞仪检测记忆性CD4+T细胞表达IFN-γ的水平,同时设立健康对照组进行比较.结果 生殖器疱疹复发患者(RGH)组血清IL-23、IL-12、IFN-γ水平明显低于健康对照组,IL-14、IL-10水平明显高于健康对照组,差异有统计学意义(P<0.01);IL-23剂量依赖性诱导患者单个核细胞(PBMC)产生IFN-γ,并刺激记忆性CD4+T细胞表达IFN-γ.结论 IL-23等Th1型细胞因子在RGH患者体内水平低下,Th1/Th2平衡失调,机体不能有效抑制病毒,是导致病情反复的重要原因;IL-23可能在体内同样刺激T细胞产生IFN-γ,发挥抗病毒效力.     

IL-17、IFN-γ在内毒素诱导性葡萄膜炎大鼠模型中的表达及其意义

[目的]探讨白介素-17(IL-17)mRNA及干扰素-γ(IFN-γ)mRNA在内毒素诱导性葡萄膜炎大鼠模型中的表达及其意义.[方法]将40只SD大鼠分为空白对照组(n=10)与模型组(n=30),模型组均采用内毒素诱导葡萄膜炎大鼠模型,并在注药6h、12h、18h、24h、48h、60h分别处死5只大鼠,对照组注射磷酸缓冲盐溶液(PBS)24h后处死10只,均取双眼葡萄膜视网膜组织,采用反转录聚合酶链式反应法(RT-PCR)检测两组眼部组织IL-17mRNA及IFN-γmRNA水平,显微镜下观察眼部组织炎症情况并按相关标准予以评分.[结果]①模型组造模不同时间眼部炎症评分均高于空白对照组(P<0.05),模型组造模6h、12h、18h后炎症评分依次升高(P<0.05),18h到达峰值,然后24h、48h、60h依次降低(P<0.05);②模型组造模不同时间IL-17 mRNA、IFN-γ mRNA水平均高于空白对照组(P<0.05),模型组造模6h、12h、18h后IL-17mRNA、IFN-γ mRNA水平依次升高(P<0.05),18h到达峰值,然后24h、48h、60h依次降低(P<0.05).[结论]葡萄膜炎大鼠眼部组织中IL-17 mRNA、IFN-γ mRNA表达水平上调,与病变程度呈一致,IL-17、IFN-γ均可能参与葡萄膜炎的发病过程.     

Enhanced demethylation of interferon-γ gene promoter in peripheral blood mononuclear cells is associated with acute-on-chronic hepatitis B liver failure

Acute-on-chronic hepatitis B liver failure (ACHBLF) refers to liver failure occurring in patients with chronic hepatitis B (CHB) related liver diseases. Interferon-γ (IFN-γ) plays an important role in the exacerbation of liver function. However, the exact mechanism, by which IFN-γ mediates ACHBLF, is not fully understood. Forty patients with ACHBLF, fifteen patients with CHB and ten healthy controls were included in this present study. ELISA was performed to measure the level of serum IFN-γ. The methylation status of IFN-γ promoter in peripheral blood mononuclear cells (PBMCs) was determined using methylation-specific PCR. Model for End-stage Liver Disease (MELD) scoring was performed for evaluating the severity of liver failure. The serum level of IFN-γ in patients with ACHBLF or CHB was significantly lower than that in healthy controls, while the serum IFN-γ level in ACHBLF patients was significantly higher than that in CHB patients. In ACHBLF patients, the level of IFN-γ was positively correlated with total bilirubin and MELD score, but negatively correlated with prothrombin time activity. These results suggest the involvement of IFN-γ in the pathogenesis of ACHBLF. Importantly, the degree of methylation of the IFN-γ gene promoter in ACHBLF patients (60%, 24/40) was significantly lower than that in CHB patients (93%, 14/15), but was higher than that in the control group (20%, 2/10). Furthermore, in ACHBLF patients, the serum IFN-γ level was significantly higher in unmethylation group than that in methylation group. In conclusion, enhanced demethylation of IFN-γ gene promoter in PBMCs may be associated with the onset of ACHBLF.     

肺结核合并2型糖尿病患者血清炎性细胞因子的表达及其临床意义

目的观察并初步分析肺结核合并2型糖尿病患者血清中炎性细胞因子白细胞介素-2(IL-2)、白细胞介素-10(IL-10)、γ干扰素(IFN-γ)、肿瘤坏死因子(TNF-α)的表达及临床意义。方法采用前瞻性研究,选取2015年11月至2016年11月海安市人民医院收治的2型糖尿病患者51例作为DM组,肺结核合并2型糖尿病患者48例作为TB+DM组,同期的健康体检者42例作为对照组。采集研究对象清晨空腹静脉血5 ml,采用酶联免疫吸附法检测各样本血清中IL-2、IL-10、IFN-γ、TNF-α的水平,进行对比。结果三组间的IL-2、TNF-α、INF-γ、IL-10水平比较,差异均具有统计学意义(P<0.05)。其中,TB+DM组的IL-2(0.215±0.152)μg/L、TNF-α(0.253±0.098)μg/L水平均低于DM组[(1.058±0.458)μg/L、(0.399±0.102)μg/L],INF-γ(33.560±2.370)μg/L、IL-10(1.331±0.241)μg/L水平均高于DM组[(6.010±6.730)μg/L、(0.835±0.301)μg/L],差异具有统计学意义(P<0.05)。TB+DM组和DM组的IL-2、TNF-α水平均低于对照组,INF-γ、IL-10水平均高于对照组,差异具有统计学意义(P<0.05)。结论检测患者血清中IL-2、IL-10、IFN-γ、TNF-α的水平对选取肺结核合并2型糖尿病指标诊断、评价该病的发生及病程,对治疗该病有着重要意义。