9-顺维甲酸对人肺癌组织CyclinD1、Cdk4、P16转录的影响

目的：检测9－顺维甲酸（9-cis-RA)处理前后体外培养的人肺癌组织及其对照组织细胞周期蛋白因子CyclinD1、细胞周期蛋白依赖性激酶Ddk4、细胞周期蛋白酶抑制因子P16转录水平的变化，探讨其抑制肺癌组织细胞生长的作用机制。方法：采用半定量逆转录聚合酶链式反应（RT－PCR）分析CyclinD1,Cdk4,P16转录的变化，结果：9-cis-RA既可降低体培养的人肺癌组织Cdk4的转录，又可升高其P16的转录，对肺癌组织和正常肺组织CyclinD1的转录的影响无统计学意义。对正常肺组织Cdk4,P16的转录的影响也无统计学意义。结论：9-cis-RA抑制肺癌组织细胞增殖的作用机理与其调节Cdk4,P16的转录有关。     

9-顺-维甲酸对非小细胞肺癌CyclinD1、Cdk4表达的影响

目的:研究9-顺-维甲酸对非小细胞肺癌A549、PG、SPC-A1的细胞周期蛋白D1(cyclinD1)、细胞周期蛋白依赖性蛋白激酶4(Cdk4)表达的影响,探讨其抑制非小细胞肺癌生长与影响CyclinD1、Cdk4表达的相关性。方法:采用半定量逆转录PCR方法,分析细胞周期因子CyclinD1、Cdk4表达的变化。结果:9-顺-维甲酸既可以降低PG、SPC-A1的CyclinD1表达(P<0.01),又可以降低PG、A549的Cdk4表达(P<0.01)。结论:9-顺-维甲酸对非小细胞肺癌生长的抑制作用,与其调控细胞周期因子CyclinD1、Cdk4的表达密切相关。     

9-顺-维甲酸对人肺鳞癌细胞分化和凋亡的影响

目的研究9-顺-维甲酸(9-cis-RA)对人肺鳞癌L78细胞、A2细胞分化和凋亡的影响.方法以1和5μmol/L 9-cis-RA处理肿瘤细胞株,分别于0、12、24、36、48、72h检测对照组和用药组细胞数及细胞HE染色后的形态变化,应用流式细胞术分析细胞周期分布、Bcl-2表达和凋亡细胞数.结果9-cis-RA可抑制肺鳞癌细胞生长;用药后细胞分化征明显,可见凋亡细胞.流式细胞计数显示,用药组细胞G0/G1百分率明显升高,S期细胞百分率下降,细胞凋亡率增加,Bcl-2的表达下降.结论9-cis-RA能抑制人肺鳞癌L78细胞、A2细胞增殖,诱导细胞分化和凋亡.     

9—顺—维甲酸对非小细胞肺癌CyclinDl、Cdk4表达的影响

目的研究9-顺-维甲酸对非小细胞肺癌A549、PG、SPC-A1的细胞周期蛋白D1(cyclinD1)、细胞周期蛋白依赖性蛋白激酶4(Cdk4)表达的影响,探讨其抑制非小细胞肺癌生长与影响CyclinD1、Cdk4表达的相关性.方法采用半定量逆转录PCR方法,分析细胞周期因子CyclinD1、Cdk4表达的变化.结果9-顺-维甲酸既可以降低PG、SPC-A1的CyclinD1表达(P＜0.01),又可以降低PG、A549的Cdk4表达(P＜0.01).结论9-顺-维甲酸对非小细胞肺癌生长的抑制作用,与其调控细胞周期因子CyclinD1、Cdk4的表达密切相关.     

9—顺—维甲酸对人肺鳞癌细胞分化和凋亡的影响

目的：研究9－顺－维甲酸（9－cis-RA)对人肺鳞癌L78细胞,A2细胞分化和凋亡的影响,方法：以1和5umol/L 9-cis-RA处理肿瘤细胞株,分别于0,12,24,36,48,72h检测对照组和用药组细胞数及细胞HE染色后的形态变化,应用流式细胞术分析细胞周期分布,Bcl-2表达和凋亡细胞数,结果：9－cis-RA可抑制肺鳞癌细胞生长,用药后细胞分化征明显,可见凋亡细胞,流式细胞计数显示,用药组细胞G0/G1百分率明显升高,S期细胞百分率下降,细胞凋亡率增加,Bcl-2的表达下降,结论0－cis-RA能抑制人肺鳞癌L78细胞,A2细胞增殖,诱导细胞分化和凋亡。     

平阳霉素胸膜固定术中PAI-1、t-PA和TGF-β_1的意义

目的观察4-氨基-2-三氟甲基苯基维甲酸酯(ATPR)对人肺腺癌细胞A549和鳞癌细胞L78生长抑制、分化的影响。方法将两株细胞分为ATPR组、空白对照组及全反式维甲酸(ATRA)组。前组分别以0.01、0.1、1、5、10μmol/L的ATPR与肺腺癌细胞A549和鳞癌细胞L78作用24、48、72、96 h。运用MTT法检测细胞吸光度并计算细胞的生长抑制率,流式细胞仪检测细胞周期。0.1、1、5μmol/L的ATPR与肺腺癌细胞A549和鳞癌细胞L78作用72 h,苏木精-伊红(HE)染色观察肺癌细胞形态变化。1μmol/L的ATPR作用肺癌细胞72 h后,RT-PCR法检测细胞核维甲酸α受体(RARα)、表皮生长因子受体(EGFR)表达的变化。结果 ATPR具有抑制A549、L78细胞生长的作用,生长抑制率有剂量和时间依赖关系。流式细胞仪分析结果显示A549、L78细胞被阻滞于G0-G1期。A549、L78细胞在ATPR的作用下,发生了细胞胞体变小、分裂状态的细胞数减少等形态学变化。通过对肺癌细胞的RT-PCR法检测,均发现EGFR和RARα的特异性条带。空白对照组L78细胞高表达EGFR;A549细胞...     

9-顺维甲酸对肺鳞、腺癌细胞株C-erbB-2和Rb表达的影响

目的:探讨9-顺-维甲酸(9-cis-RA)的抗肿瘤机理,为临床治疗肺癌提供理论依据.方法:体外培养肺癌细胞株A2、L78、A459,随机分组.实验组加5μmol/L9-cisRA,对照组不加9-cis-RA继续培养,48h后取出,用免疫组化SABC法分别检测两组c-erbB-2和Rb的基因表达并进行统计学分析.结果:①实验组、对照组A2株中P185表达率无明显差异(P＞0.05),L78株和A549株P185表达率差异非常显著(P＜0001);②A2、A549实验组、对照组Rb未见表达,L78实验组Rb表达率9.8%,对照组Rb无表达,差异显著(P＜0.01).结论:①9-cis-RA通过降低c-erbB-2表达,促使肺癌细胞株分化,抑制肺癌细胞株生长;②9-cis-RA增强L78细胞株抑癌基因Rb的表达,抑制肺癌细胞株的增殖,发挥其抗癌作用;③9-cis-RA抑制原癌基因c-erbB-2的同时也激活抑癌基因Rb,协同发挥抗癌作用;④9-cis-RA处理后L78细胞株中Rb表达增加可能与诱导细胞凋亡有关.     

9－顺维甲酸对鳞癌细胞生物学特性的影响

目的：探讨9－顺维甲酸（9－cis-RA)对肺鳞癌细胞的生物学作用。方法：对应用9－cis-RA处理后L78和A2两肺鳞癌细胞株的生长,分化和凋亡等特性进行检测观察。结果：（1）9－cis-RA对两肺癌细胞株的生长产生明显的抑制作用。（2）应用9－cis-RA后,两株细胞分化特征明显;（3）经9－cis-RA处理后的两肺鳞癌细胞凋亡率明显高于对照组。结果：9－cis-RA具有抑制肺鳞癌细胞的生长,诱导其分化和凋亡等生物学作用。     

9—顺维甲酸对人肺腺癌细胞维甲酸受体转录的影响

目的：观察9－顺维甲酸（9-cis-RA)处理前后人肺腺癌细胞株PG和A549维甲酸受体（RARs)及维甲类X受体（RXRs）不同时相mRNA转录水平的表达。方法：用RT－PCRT相对定量检测人肺腺癌细胞株PG和A549加9-cis-RA前后RARs和RXRs mRNA转录水平。结果：应用9-cis-RA后，两细胞株的RARα和RARβ mRNA转录水平较对照组明显增高，其余受体转录无明显变化。结论：受体RARα和RARβ可能与9-cis-RA对两细胞株的生长抑制和诱导凋亡作用有密切关系。     

microRNA-24通过下调Cdk4和CyclinD3抑制胰岛β细胞增殖

目的:通过共同过表达Cdk4?CyclinD3与microRNA-24,观察miR-24所引起的胰岛β细胞增殖抑制是否得到逆转,从而判定Cdk4?CyclinD3是否介导了这种抑制作用?方法:在胰岛β细胞系MIN6细胞中过表达miR-24,运用WST-1法检测细胞活力,借助于流式细胞术检测细胞周期,Hoechst33342染色和BrdU标记检测细胞增殖凋亡情况?Western blot检测Cdk4?CyclinD3蛋白水平?构建cyclinD3-pCMV5-myc,cdk4-pCMV5-myc重组质粒,与pre-miR-24?pre-Neg miRNA precursors共转染MIN6细胞,BrdU标记检测细胞增殖?结果:过表达miR-24引起MIN6细胞活力降低,细胞周期G2期阻滞,细胞增殖受到抑制而并没有显著的凋亡;过表达miR-24降低了Cdk4?CyclinD3蛋白水平;而Cdk4?CyclinD3的过表达逆转了miR-24引起的胰岛β细胞增殖抑制?结论:miR-24通过降低Cdk4?CyclinD3蛋白水平抑制胰岛β细胞增殖,该作用可被Cdk4?CyclinD3蛋白的过表达逆转?     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.