食物特异性IgG与过敏性紫癜的相关性研究

【目的】研究过敏性紫癜（HSP）与食物特异性IgG抗体水平相关性。【方法】选取HSP患儿155例作为试验组，无过敏史正常儿童36例作为对照组，采用酶联免疫吸附法（ELISA）检测两组儿童食物特异性IgG抗体水平，并比较几种不同情况下特异性IgG抗体阳性率：OHSP患儿与正常儿童；②有肾脏受累的HSP与无肾脏受累的HSP患儿；③有胃肠道受累的HSP与无胃肠道受累的HSP患儿。同时分析比较1gG抗体阳性的HSP患儿与IgG抗体阴性HSP患儿的临床各种参数的差异。【结果】①HSP患儿IgG抗体总体阳性显著率高于对照组（P〈0．05），且单项食物中牛奶，螃蟹，鸡蛋，虾IgG抗体阳性率高于对照组（P〈0．05）。②有肾脏受累与无肾脏受累的HSP患儿，IgG抗体阳性率无差别（P〉0．05）。③有胃肠道受累的HSP患儿IgG抗体阳挂率高于无胃肠道受累的HSP患儿（P〈0．05），且前者单项牛奶，鸡蛋的IgG抗体阳性率高于后者（Pd0．05），螃蟹特异性IgG阳性率显著高于后者（P〈0．01）。④IgG抗体阳性与IgG抗体阴性的HSP患儿相比较，在可能的诱因（上感，药物，食物，不明原因），发热，皮肤紫癜，关节受累，肾脏受累（血尿，蛋白尿，肾病综合征，血肌酐升高）；白细胞升高，血沉升高，IgA升高等方面无差别（P〉0．05），但在胃肠道受累（腹痛，呕吐，黑便）等方面有差异（P〈0．05）。【结论】鸡蛋，牛奶，螃蟹，虾等特异性IgG抗体与过敏性紫癜关系密切，且可能通过影响胃肠系统功能而发挥作用。     

10126例儿童血清食物特异性IgG检测分析

目的探讨天津地区儿童常见食物不耐受情况,为临床诊断儿童过敏性疾病提供理论依据。方法采用免疫印迹法检测10 126例儿童血清中食物特异性IgG抗体水平,分析常见食物不耐受阳性率与性别、年龄、季节之间的关系。结果10 126例患儿中有9 332例血清食物特异性IgG抗体阳性,阳性率为92.2%。阳性率较高的前3位依次是鸡蛋(74.2%)、牛奶(68.1%)和小麦(55.0%)。其中仅对1种食物不耐受患儿为1 260例(12.4%),对2种食物不耐受患儿为1 537例(15.2%),对2种以上食物不耐受患儿例数呈下降趋势。不同年龄段患儿血清食物特异性IgG抗体总体阳性率比较,差异有统计学意义(P0.05)。14种食物不耐受阳性率在4个年龄组之间的差异均有统计学意义(P0.05)。不同性别间食物特异性IgG抗体总体阳性率差异无统计学意义(P0.05),男童对牛奶和蘑菇不耐受阳性率略高于女童,差异有统计学意义(P0.05)。不同季节患儿血清食物特异性IgG抗体总体阳性率差异有统计学意义(P0.05)。结论鸡蛋、牛奶和小麦是天津地区导致儿童食物不耐受的主要过敏原。血清食物特异性IgG抗体检测对儿童食物不耐受的预防和诊断有一定的应用价值。     

食物特异性IgG抗体检测在过敏性紫癜中的应用分析

目的 探讨食物不耐受与过敏性紫癜的相关性.方法 采用ELISA方法检测63例过敏性紫癜患者血清中14种食物特异性IgG抗体.结果 63例过敏性紫癜患者中有44例检测到1～12种食物特异性IgG阳性升高,总阳性率为69.8%(44/63);特异性IgG阳性率在10%以上的主要致敏食物有5种,阳性率由高至低分别为牛奶(34.9%),鸡蛋(30.2%),小麦(23.8%),螃蟹(11.1%),大豆(11.1%);食物特异性IgG浓度分级结果表明,多数食物以引起轻度不耐受(即IgG浓度为+1级)的病例为主;患者食物不耐受检出阳性率以及14种食物IgG阳性率在不同性别间差异均无统计学显著性意义(P>0.05);食物不耐受阳性患者中,对1～2种食物不耐受者所占比例较多(分别为45.4%和36.4%).结论 过敏性紫癜患者食物不耐受发生率较高,食物不耐受与过敏性紫癜具有密切相关性.食物特异性IgG抗体检测对过敏性紫癜防治将具有重要意义.     

1280例过敏性鼻炎食物不耐受检测结果分析

目的探讨血清中7种食物特异性IgG抗体与过敏性鼻炎的相关性。方法采用酶联免疫吸附法检测1280例过敏性鼻炎患者血清中7种食物特异性IgG抗体水平。结果患者血清食物特异性IgG抗体总阳性率为81.14%,7种食物特异性IgG抗体有不同程度增高,以鸡蛋(62.27%)阳性率最高,其次为鱼(58.20%)、牛奶(47.27%);不同年龄组过敏食物比较有特异性。结论此类患者体内产生特异性IgG抗体,提示过敏性鼻炎与食物不耐受有关,对临床诊治和防护有重要意义。     

过敏性紫癜患儿食物不耐受检测及其意义

目的探讨过敏性紫癜患儿食物不耐受血清特异性IgG水平对其病因诊断及饮食治疗的意义。方法用食物不耐受IgG体外检测试剂盒检测30例过敏性紫癜患儿和20例健康对照儿童的血清特异性IgG水平。结果实验组30例过敏性紫癜患者的血清总IgG阳性率为76.67%,其中对1种食物不耐受为3例,对2种以上食物不耐受者为20例。健康对照组20例血清总IgG阳性率为25%,对1种食物不耐受为2例,对2种以上食物不耐受者为3例,常见的引起不耐受的食物依次为牛奶、虾、鸡蛋清和鸡蛋黄、蟹、大豆、鳕鱼、大米、小麦、猪肉、牛肉等,实验组饮食上经避免不耐受食物及抗过敏治疗疗程缩短,疗效优于对照组。结论血清特异性IgG水平检测对过敏性紫癜的病因诊断有参考意义,对患儿饮食治疗及营养状态的改善具有重要的指导意义。     

14种食物不耐受血清特异性IgG抗体检测在临床中的应用价值

目的分析血清中14种食物不耐受特异性IgG抗体检测在临床中的应用价值,为临床相关疾病的诊治提供依据。方法对596例样本采用酶联免疫吸附法（ELISA）半定量检测血清中14种食物特异性IgG水平。结果患者组食物不耐受阳性率和14种食物血清特异性IgG抗体浓度均高于健康对照组,差异有显著性意义（P〈0.05）;4组症状组平均阳性率以蛋清/蛋黄最高（50.2%）,其次为蟹（36.7%）、虾（31.4%）、牛奶（23.0%）;平均IgG浓度也以蛋清/蛋黄最高（205.4U/ml）,其次为牛奶（196.9U/ml）、西红柿（121.3U/ml）、猪肉（116.8U/ml）。结论患者体内产生食物特异性IgG抗体,提示患者症状与食物不耐受有关。及时明确不耐受食物,对临床相关疾病的诊治和预防具有一定的价值。     

上海地区2437例食物不耐受患儿过敏原谱检测分析

目的探讨上海市食物不耐受患儿过敏原谱的分布特征,为该地区儿童食物不耐受疾病的防治提供科学依据。方法选取进行食物过敏原检测的患儿2 437例,采用酶联免疫吸附试验(ELISA)检测患儿血清食物特异性IgG抗体,对检测结果进行统计分析。结果 2 437例食物不耐受患儿中有1 928例(79.1%)血清食物特异性IgG抗体阳性。最主要的食入性过敏原为蛋清/蛋黄,阳性率为54.7%,之后依次为小麦(44.3%)、西红柿(31.3%)、牛奶(30.5%),猪肉、牛肉阳性率最低(均1%)。男童的蛋清/蛋黄阳性率明显高于女童(P0.05),而女童的蘑菇阳性率显著高于男童(P0.05)。不同年龄段患儿牛奶、蛋清/蛋黄、小麦、玉米、鳕鱼、大米、西红柿、蟹、虾和鸡肉阳性率差异有统计学意义(P0.05);随着年龄的增长,部分食物特异性IgG抗体阳性率呈下降趋势。结论蛋清/蛋黄、小麦、西红柿和牛奶是上海地区儿童食物不耐受疾病的主要过敏原。通过检测食物特异性IgG抗体可以明确病因,对食物不耐受患儿的个体化治疗和健康教育有重要作用。     

过敏性紫癜患儿食物不耐受的检测分析

目的：探讨过敏性紫癜（HSP）患儿食物不耐受情况。方法采用ELISA,检测比较该地区58例HSP患儿以及23名正常儿童血清中14种食物特异性IgG （sIgG）水平,并分析不同性别、年龄患儿的不耐受阳性率及不耐受食物的分布差异。结果58例患儿食物 sIgG 阳性率为84．5％,高于对照组的13．0％,比较差异有统计学意义（P＜0．01）。HSP患儿不耐受的食物前4位依次为：鸡蛋/蛋白蛋黄（70．7％）、牛奶（53．4％）、鳕鱼（19．0％）、螃蟹（10．3％）;其中1种食物sIgG阳性18例（31．0％）,2种或2种以上食物sIgG阳性31例（53．4％）。不同性别、不同年龄[低龄儿童（＜7岁）及高龄儿童（≥7岁）]食物sIgG总阳性率比较差异无统计学意义（P＞0．05）,高龄儿童的螃蟹IgG阳性率较低龄儿童高（P＜0．01）。结论HSP患儿食物不耐受情况较正常儿童明显。该组HSP患儿对鸡蛋/蛋白蛋黄、牛奶、鳕鱼、螃蟹不耐受情况较为明显,可作为地区参考,高龄儿童对螃蟹的不耐受情况较低龄儿童明显。     

过敏性皮肤病与食物不耐受血清特异性IgG的相关性分析

目的探讨三种过敏性皮肤病与患者血清中食物小耐受特异性IgG的关系方法应用酶联免疫吸附法检测316例过敏性皮肤病患者血清中食物特异性IgG抗体水平。结果316例患者血清中食物不耐受特异性IgG阳性率占96．5％,其中以鸡蛋最高（49．1％）,其次为虾、牛奶、螃蟹和鳕鱼。结论食物特异性IgG的检测,为过敏性皮肤病的传统诊断和治疗提供了一种新的方法。     

过敏性皮肤病与食物不耐受血清特异性IgG的相关性分析

目的 探讨三种过敏性皮肤病与患者血清中食物不耐受特异性IgG的关系.方法 应用酶联免疫吸附法检测316例过敏性皮肤病患者血清中食物特异性IgG抗体水平.结果 316例患者血清中食物不耐受特异性IgG阳性率占96.5%,其中以鸡蛋最高(49.1%),其次为虾、牛奶、螃蟹和鳕鱼.结论 食物特异性IgG的检测,为过敏性皮肤病的传统诊断和治疗提供了一种新的方法.     

儿童过敏性紫癜急性期特异性IgE抗体检测价值及其病因研究

目的 通过时无锡地区儿童过敏性紫癜患者急性期过敏物质的检测,了解其过敏状态及其过敏原构成情况,从而采取相应对策.方法 选择246例急性发作期过敏性紫癜患者作为检测对象,均采用体外过敏原检测的方法,以德国AllergyScreen系统作为检测平台,检测血清特异性IgE和总IgE水平.结果 246例过敏性紫癜患者中,血清过敏原特异性抗体IgE阳性反应者120例,占48.7%;其中对两种及两种以上过敏原反应阳性者86例,占34.9%;对一种过敏原反应阳性者34例,占13.8%.血清总IgE阳性(＞100 kU/L)者194例,占78.9%;吸入组过敏原反应阳性者134例,占54.5%;其中尘螨、粉螨(19.1%),屋尘(17.1%),点青霉、交链孢霉(10.2%)较高.食物组过敏原反应阳性者77例,占31.3%;其中鱼肉(6.5%),蛋黄、蛋白(5.7%),羊肉(5.3%)较高.吸入组和食物组过敏原阳性率差异无统计学意义(χ2=2.59,P＞0.05).结论 过敏性紫癜患者急性期过敏原多呈混合性,吸入类与食物类过敏原均与过敏性紫癜的发生有关,故需对相应的过敏原进行必要的避免,以减轻过敏原所带来的危害.     

血清补体C1q、RBP及尿mALB联合检测在儿童过敏性紫癜早期肾损伤中的临床意义

目的 探讨血清补体C1q、视黄醇结合蛋白(RBP)及尿微量清蛋白(mALB)联合检测在儿童过敏性紫癜(HSP)早期肾损伤中的临床意义.方法 选取该院2017年7月至2018年12月住院的70例HSP患儿作为观察组,其中单纯型HSP 50例,合并紫癜性肾炎(HSPN)20例;另选取同期该院健康体检儿童30例作为对照组.对...     

440例患者食物过敏原特异性IgG抗体浓度分析

目的检测食物不耐受患者血清中14种食物特异性IgG抗体并分析食物不耐受的原因。方法采用酶联免疫吸附试验检测440例患者血清中14项食物特异性IgG抗体。其中儿童组220例、成人组220例。结果在检测的14种食物特异性IgG抗体中,牛奶和鸡蛋阳性率最高;总阳性率儿童组明显高于成人组,差异有统计学意义(P<0.05)。结论通过14项食物特异性IgG抗体的浓度检测分析,各种人群都存在食物不耐受现象,儿童与成年女性最为常见。     

Association between Helicobacter pylori infection and food-specific immunoglobulin G in Southwest China

BACKGROUND Helicobacter pylori (H. pylori) has been found to be associated with extragastrointestinal diseases, possibly including adverse food reactions (such as food allergy or intolerance). However, there are few studies on H. pylori and food allergy or intolerance, and the results are inconsistent. Food-specific immunoglobulin (Ig) G has been revealed to be associated with food allergy or intolerance and can be used as a marker to explore the correlation between H. pylori infection and food allergy or intolerance.AIMTo explore the relationship between H. pylori infection and food-specific IgGMETHODSWe retrospectively analyzed the physical examination data of 21822 subjects from February 2014 to December 2018 in this study. H. pylori infection was detected using the ¹³C urea breath test. Food-specific IgG of eggs, milk and wheat in serum was assessed. Subjects were grouped according to H. pylori positivity, and the positive rates of three kinds of food-specific IgG were compared between the two groups. Multivariable logistic regression analysis was performed to elucidate the association between H. pylori infection and food-specific IgG.RESULTSThe total infection rate of H. pylori was 39.3%, and the total food-specific IgG-positive rates of eggs, milk and wheat were 25.2%, 9.0% and 4.9%, respectively. The infection rate of H. pylori was higher in males than in females, while the positive rates of food-specific IgG were lower in males than in females. The positive rates of food-specific IgG decreased with age in both males and females. In the H. pylori-positive groups, the positive rates of food-specific IgG of eggs, milk and wheat were all lower than those in the H. pylori-negative groups. Multivariate logistic regression analysis revealed that H. pylori infection was negatively correlated with the food-specific IgG-positive rates of eggs, milk and wheat (odds ratio value of eggs 0.844-0.873, milk 0.741-0.751 and wheat 0.755-0.788, in different models).CONCLUSION H. pylori infection was found to be negatively associated with the food-specific IgG of eggs, milk and wheat in Southwest China.     

Specific humoral response to cows’ milk proteins and ovalbumin in children with atopic dermatitis

Summary Serum antibodies to four common food antigens, three cows’ milk proteins (casein, α-lactalbumin and β-lactoglobulin) and ovalbumin, were investigated in 21 children with atopic dermatitis (aged 3 months to 3 years) and in 15 age-matched healthy controls. Specific IgE was measured by radioallergosorbent test; an ELISA was developed to detect specific IgG, IgG subclasses and IgA. Specific IgE was found in 76% of patients, while antigen-directed IgG and IgA were present both in patients and healthy controls; IgG to ovalbumin and IgA to α-lactalbumin were significantly higher in children with atopic dermatitis. The analysis of the IgG subclass distribution showed different patterns of response, IgG1 and IgG4 being higher in patients (even though statistically significant only for ovalbumin), and IgG2 and IgG3 being lower in this group. The presence of food-specific IgE in the majority of atopic children and the different specific IgG subclass patterns observed in patients and controls may reflect an alteration in the immune response to dietary proteins in atopic dermatitis.     

间接免疫荧光试验在人群埃可30型肠道病毒IgG抗体检测中的应用

目的建立埃可30型肠道病毒(Echovirus30,Echo30)国内流行株血清特异性IgG抗体检测的间接免疫荧光法(IFA),了解人群既往感染情况。方法采用由爆发人群分离得到的病毒株感染人胚肺二倍体细胞(MRC-5)制备抗原片,探讨IFA检测血清Echo30 IgG抗体的方法,并初步用于检测苏北病毒性脑膜炎爆发后收集的该地区儿童血清中Echo30 IgG抗体。结果建立了Echo30肠道病毒血清特异性IgG抗体的IFA检测方法。在44份健康儿童血清中筛检到28份(63.6%)Echo30 IgG抗体阳性,3份患者血清Echo30 IgG抗体均为阳性。结论Echo30国内流行株IgG抗体的IFA操作简便、经济,可用于血清流行病学调查。爆发地区儿童中存在大量的无症状感染者。     

Children with atopic eczema. II: Immunological findings associated with dietary manipulations.

A group of 91 children with atopic eczema entered a study where clinical and immunological features were compared before and after a food elimination diet, and after double blind randomized food challenges in which a food was given for several days at a time. Eczema improved significantly during the diet and became worse on food challenges. The clinical outcome of food elimination could not be predicted by the initial skin prick test results, serum immunoglobulins, total or food-specific IgE, or complexed IgG or IgE. There was a tendency for patients whose eczema did not improve after food elimination to have higher initial serum IgG levels, without a corresponding increase in skin infections. The radioallergosorbent test to soy gave a higher result in those who improved on diet. No significant changes in serum or complexed immunoglobulins occurred over the period of food elimination or subsequent food challenge. Results of food challenges could not be predicted by initial serum immunoglobulin levels. A history of spring/summer exacerbations of eczema correlated with positive skin prick tests to silver birch pollen, but not to grass pollen. Serological tests did not help in planning food diets in atopic eczema, and the immunological studies did not delineate any particular mechanism by which foods might exacerbate eczema.     

The effects of age on isotypic antibody responses to Aspergillus fumigatus: implications regarding in vitro measurements

The demonstration of elevated serum immunoglobulin E (IgE), immunoglobulin G (IgG), and immunoglobulin A (IgA) antibodies to Aspergillus fumigatus (Af) is a valuable aid in the serodiagnosis of allergic bronchopulmonary aspergillosis (ABPA). It is important to recognize cases of ABPA, because repeated untreated episodes of pulmonary infiltrates can result in bronchiectasis and even fatal fibrotic lung disease. Serum samples from patients with ABPA have increased isotypic antibodies to Af when compared with patients with asthma and immediate cutaneous reactivity but in whom ABPA has been excluded. There is little information regarding age-related changes of isotypic antibodies to a specific allergen or in comparison of such changes with age-related changes in total class-specific antibody. We determined IgE-Af, IgG-Af, and IgA-Af and total IgE, IgG, and IgA concentrations in serum samples from children with asthma (ages less than 1 through 5 years, 6 through 10 years, and 11 through 15 years) and compared the results with those in children with APBA and adults with asthma. The use of an indirect amplified ELISA with Af adsorbed to microtiter wells demonstrated that serum samples from children with ABPA have elevated IgE-Af, IgG-Af, and IgA-Af concentrations compared with those in serum samples from children with asthma. The assays were sensitive and highly specific in discriminating ABPA from asthma in children. Isotypic antibodies to Af in children do not demonstrate age-related increases as occur for total serum IgE, IgG, and IgA concentrations.(ABSTRACT TRUNCATED AT 250 WORDS)     

Clinical associations with serum allergen-specific IgG4 antibodies

Serum total and allergen-specific IgE and IgG4 antibodies, were measured in eighty-seven atopic and nineteen non-atopic asthmatics. The allergens studied were: Dermatophagoides pteronyssinus, grass pollens, cat dander, dog dander, milk and egg. Sixty-eight atopic asthmatics and fourteen non-atopic asthmatics were found to have allergen-specific IgG4 antibodies, to at least one of the allergens tested. IgG4 antibodies to milk and egg were common in both groups of asthmatics, and to animal danders in the non-atopic asthmatics. Skin prick tests were always negative when allergen-specific IgG4 antibodies occurred alone, but in such cases, intradermal skin tests were positive. Seventy-five per cent of a group of patients with normal levels of serum total IgG4, were found to have at least one positive IgG4 RAST.