Chemical Constituents of Euphorbia ebracteolata

Aim To study the chemical constituents of Euphorb/a ebracteolata Hayata. Methods Column chromatography was used in the isolation procedure, while the structures of isolated compounds were elucidated by spectral data. Results Six compounds were isolated and their structures were identified as baccatin (1), 3-acetyl-β-amyrin (2), 3,3‘-diacetyl-4,4‘-dim-ethoxy- 2,2‘, 6,6‘-tetrah ydroxy diphenylmethane (3), 2,4- dihydroxy-6-methoxy-3-methyl acetophenone (4), β-sitosterol（5), and daucosterol (6). Conclusion Baccatin was obtained from Euphorbia ebracteolata for the first time.     

小红参的化学成分研究

Six compounds were isolated from the ethyl ether extract of Rubia yunnanensis(Franch)Diels. Based on spectroscopic means(UV,IR,MS,¹H-NMR and ¹³C-NMR)five of themwere identified as rubiarbonol A,rubiarbonol G,rubiarbonone A,1,3,6-trihydroxy-2-methylanthr-aquinone β-sitosterol and one new compound identified as 7β,19α,28-trihydroxyarbor-9(11)-3-one,named rubiarbonone B.     

北马兜铃的化学成分研究——Ⅱ、马兜铃酸E的化学结构

From the root of Aristolochia contorta Bunge six chemical constituents were isolated; one of them is a new phenanthrene compound containing nitro group for which the name aristolochic acid E is suggested. By means of spectral methods combined with chemical analysis the chemical structure of aristolochic acid E was determined to be 7-methoxy-8-hydroxy-aristolochic acid. The other components were identified as allantoin, aristolochic acid A, magnoflorine, β-sitosterol and daucosterol respectively.     

A Monoterpene Glycoside from Ehinacea purpurea

Aim To separate and identify chemical constituents of Ehinacea purpurea. Methods Five compounds were isolated from the plant using chromatography. Their structures were elucidated by spectroscopy. Results Five compounds were isolated and their structures were identified as 2, 6-dimethyl-7-octene-2, 3, 6-triol-2-O-β-D-gtucopyranoside (1), 7, 8-furocoumarin (2), 6-methoxy-7-hydroxycoumarin (3), caffeic acid (4), methyl catfeate (5), and ethyl catfeate(6). Conclusion All these compounds were obtained from the plant for the first time.     

槲寄生化学成分的研究——Ⅷ.2,3-丁二醇单葡萄糖甙的分离和结构

From the ethanol extract of Viscum coloratum (Kom.) Nakai , a giucoside ofaliphatic diol and three other glucosides were isolated. Based on chemical and spectroscopic analysis,the structures have been elucidated as 2-β-D- glucosyl-3- methylpropanol (Ⅷ), syringin (Ⅸ),eleatheroside E(Ⅹ) and syringenin-4'-O-D-apiosylglucoside (Ⅺ). Ⅷ is a new glucoside of aliphaticdiol and named 3-β-D-glucopyranosyloxy-butanol-2. Three other compounds (Ⅸ～-Ⅺ) were foundfor the first time in this plant.     

薯蓣属植物化学成分的研究——Ⅹ.蜀葵叶薯蓣中甾体皂甙和皂甙元的分离和鉴定

Four components were isolated from acid-treated rhizome of Dioscorea althaeoides Kunth. Their structures were identified as diosgenin (Ⅰ), β-sitosterol (Ⅱ), diosgenin palmitate (Ⅲ) and △^3,5-deoxytigogenin (Ⅳ) on the basis of physical constants and spectral data.Two sparingly soluble steroid saponins were also isolated from this plant Their physicochemical constants showed that one of them is dioscin (Ⅴ) and the other graeillin (Ⅵ).     

豹皮菌中2-Amino-3-(1,2-dicarboxyethylthio)propanoic acid非对映异构体的绝对构型

Four stereoisomers of 2-amino-3-(1,2-dicarboxyethylthio) propanoic acid were prepared by reaction of L- and D-cysteine with fumaric acid. The absolute configuration of the diastereoisomer of 2-amino-3-(1,2-dicarboxyethylthio) propanoic acid from Amanita pantherina were assigned as (2R, 1'R) and (2R, 1'S) by analysis of the optical properties. Pharmacological tests showed that all of the four stereoisomers inhibited the depolarization of NMDA on spinal motorneurones in newborn rats, The inhibition intensity of L-A,D-A and D-B were higher than that of L-B.     

马蓝的化学成分研究

Seven compounds have been isolated from the whole plant of Strobilanthes cusia (Nees) O. Ktze. Three of them are triterpenes (Ⅰ～Ⅲ), two are indole alkaloids (Ⅳ, Ⅴ), two are quinazolinone alkaloids (Ⅵ, Ⅶ). On the basis of spectral analysis and physicochemical properties, their structures were established as lupeol (Ⅰ), betulin (Ⅱ), lupenone (Ⅲ), indigo (Ⅳ), indirubin (Ⅴ), 4 (3H)-quinazolinone (Ⅵ), 2, 4 (1H, 3H)-quinazolinedione (Ⅶ). Ⅵ and Ⅶ were found from natural plant for the first time.The results of the pharmacological tests demonstrate that compound Ⅴ has anticancer activity and compound Ⅵ has hypotensive action. Compound Ⅶ can be quantitatively determined by HPLC, which may serve as a quality control standard for materia medica and its preparations. Compounds Ⅵ and Ⅶ have been confirmed by means of synthesis.     

抗心肌缺血药3-{4-[(2-羟基-3-烷氨基)丙氧基]苯基(苄基)}-取代4(3H)-喹唑酮的合成

In order to search for effective antimyocardial ischemic agents, fourteen new 3 4-[(3-alkylamino-2-hydroxy)propoxy] phenyl(benzyl)]-substituted 4(3H)-quin zolinones (Ⅱ) were synthesized. Substituted o-aminobenzoic acids used as the starting materials were allowed to react with acetic anhydride and then p-aminophenol (method A), or with N- (4- hydroxyphenyl)formamide (method B), or with thionyl chloride and then N - (4 - hydroxybenzyl) formamide (methode C) to form 3-[(4-hydroxyphenyl(benzyl)]-substituted 4(3H)-quinazolinones (Ⅲ). The intermediate Ⅲ reacted with epichiorohydrin to form the epoxides (Ⅳ). The reaction of Ⅳ with an excess of isopropylamine or tert-butylamine in boiling chloroform gave the desired products. Of all the compounds synthesized, Compounds Ⅱ_3～5 and Ⅱ₁₃ were found to increase the tolerance of mice to hypoxia. Further evaluation is in progress.     

中药赤芍中化学成分的研究

Two new monoterpene glycosides were isolated from the ethanolic extract of the root of Paeonia laetiflora Pall. The structure of compound (Ⅰ) has been identified as (Z)-(1S, 5R)-β-pinen-10-yl β-vicianoside; Compound (Ⅱ) is named lactiflorin and tentatively assigned as (Ⅱ).Compound (Ⅰ) is the glycoside of the enol form of an aldehyde. In compound (Ⅱ), the sugar moiety is attached to the aglycone by two ether-like linkages. Both seem to be unprecedented.     

茵陈化学成分的分离与鉴定

目的研究茵陈的化学成分,为茵陈药材的质量控制提供一定的依据。方法采用硅胶柱色谱、羟丙基葡聚糖凝胶柱色谱、制备液相等方法进行分离,根据理化性质和1H-NMR、13C-NMR等谱学技术对分离得到的化合物进行结构鉴定。结果从茵陈中分离得到8个化合物,分别鉴定为绿原酸(chlorogenic acid,1)、咖啡酸(caffeic acid,2)、异槲皮苷(isoquercitrin,3)、异鼠李素-3-O-葡萄糖苷(isorhamnetin-3-O-glucoside,4)、6,7-二羟基香豆素(6,7-dihydroxyl-coumarin,5)、熊果酸(ursolicacid,6)、齐墩果酸(oleanolic acid,7)、β-谷甾醇(β-sitosterol,8)。结论化合物5~7为首次从茵陈中分离得到。     

天师栗化学成分的研究

目的：从中药天师栗(Aesculus wilsonii Rehd.)的干燥成熟果实中寻找新的有效成分。方法：利用各种色谱技术进行纯化,根据理化性质和各种光谱技术(IR,EIMS,FABMS,¹HNMR,¹³CNMR,¹H-¹H COSY,¹³C-¹H COSY)进行结构鉴定。结果：从天师栗的95%乙醇浸膏的乙酸乙酯和己烷部分共分得5个化合物。分别鉴定为：天师酸(1),富马酸(2),乙酰谷氨酸(3),β-谷甾醇-3-O-葡糖苷(4),β-谷甾醇(5)。结论：化合物1为新化合物,2和3是从本科植物首次分得。     

Jacaranone and related compounds from the fresh fruits ofTernstroemia japonica and their antioxidative activity

Jacaranone and related compounds (1-3) were isolated, along with three triterpenes (4-6), from the fresh fruits of Ternstroemia japonica. The compounds were identified as jacaranone (1), 3-hydroxy-2,3-dihydrojacaranone (2), 3-methoxy-2,3-dihydrojacaranone (3), 3-O-acetyloleanolic acid (4), 3-O-acetylursolic acid (5), and ursolic acid (6). Jacaranone and its derivatives were isolated for the first time from Theaceae. Of the isolated compounds, compound 3 is a new compound. Jacaranone (1) exhibited weak antioxidative effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical.     

Triterpenes from <Emphasis Type="Italic">Cynomorium songaricium</Emphasis>—analysis of HCV protease inhibitory activity,quantification, and content change under the influence of heating

Inhibitory activity of the three major triterpenes from the stems of Cynomorium songaricum—ursolic acid, acetyl ursolic acid, and malonyl ursolic acid hemiester—and their related compounds were tested for their inhibitory activity on HCV protease; malonyl ursolic acid hemiester was the most potent. A HPLC–PAD (photo diode array detector)–MS method was established to quantify the contents of each triterpene in C. songaricum. Using this method, the effect heating had on the contents was also investigated. It was found that among the three triterpenes, the content of malonyl ursolic acid hemiester decreased most quickly during the heating process.     

胡蔓藤中非生物碱类化学成分的分离与鉴定

目的对胡蔓藤的非生物碱类化学成分进行研究。方法采用HPD-800大孔吸附树脂柱色谱、硅胶柱色谱和ODS中压柱色谱进行分离,采用葡聚糖凝胶(Sephadex LH-20)及制备液相进行纯化,根据理化性质和光谱学数据鉴定结构。结果得到8个化合物,分别鉴定为β-谷甾醇(β-sitosterol,1)、豆甾醇(stigmasterol,2)、胡萝卜苷(daucosterol,3)、豆甾醇-3-O-β-D-吡喃葡萄糖苷(stigmasterol-3-O-β-D-glucopyranoside,4)、熊果酸(ursolic acid,5)、没食子酸(gallic acid,6)、阿魏酸(ferulic acid,7)、原儿茶酸(protocatechuic acid,8)。结论化合物1~8均为首次从该属植物中分得。     

白花蛇舌草的化学成分

目的对中药白花蛇舌草(Hedyotis diffusaWilld.)化学成分进行分离鉴定。方法采用反复硅胶柱色谱、Sephadex LH-20柱色谱、制备薄层色谱、重结晶等方法进行分离纯化,并通过理化常数测定和光谱数据分析鉴定其化学结构。结果分离得到7个化合物,分别鉴定为β-谷甾醇(β-sitosterol,1)、胡萝卜苷(daucosterol,2)、熊果酸(ursolic acid,3)、2-羟基-3-甲基-1-甲氧基蒽醌(2-hydroxy-3-methyl-1-methoxyanthraquinone,4)、2-羟基-7-甲基-3-甲氧基蒽醌(2-hydroxy-7-methyl-3-methoxyanthraquinone,5)、E-6-O-对甲氧基肉桂酰基鸡屎藤苷甲酯(E-6-O-p-methoxycinnamoyl scandoside methyl ester,6)、E-6-O-香豆酰基鸡屎藤苷甲酯(E-6-O-p-coumaroyl scandoside methyl ester,7)。结论化合物5为首次从该属植物中分离得到。     

野蔷薇根化学成分的研究

野蔷薇根系蔷薇科植物多花蔷薇(Rosa multiflora Thunb.)的干燥根,民间用其除风湿、活气血和治疗乳糜尿。Takahashi等从野蔷薇根中分离出2α,19α-二羟基熊果酸(tomentic acid)。我们从野蔷薇根中分离出三个化合物,通过光谱和化学反应分别鉴定为β-谷甾醇,2α,19α-二羟基熊果酸,和2α,19α-二羟基熊果酸-(28-1)-β-D-     

A new pancreatic lipase inhibitor isolated from the roots of <Emphasis Type="Italic">Actinidia arguta</Emphasis>

A new coumaroyl triterpene, 3-O-trans-p-coumaroyl actinidic acid (1), as well as five known triterpenes, ursolic acid (2), 23-hydroxyursolic acid (3), corosolic acid (4), asiatic acid (5) and betulinic acid (6) were isolated from an EtOAc-soluble extract of the roots of Actinidia arguta. The structure of compound 1 was elucidated from interpretation of the spectroscopic data, particularly by extensive 1D and 2D NMR studies. All the isolates (1-6) were evaluated in vitro for their inhibitory activities on pancreatic lipase (PL). Of the isolates, the new compound 1 possessed the highest inhibitory activity on PL, with an IC(50) of 14.95 microM, followed by ursolic acid (2, IC(50) = 15.83 microM). The other four triterpenes (3-6) also showed significant PL inhibitory activity, with IC(50) values ranging from 20.42 to 76.45 microM.     

Cytotoxic triterpenes fromCrataegus pinnatifida

Bioassay-guided fractionation of Crataegus pinnatifida (Rosaceae) gave two cytotoxic ursane-type triterpenes which were identified as uvaol (1) and ursolic acid (2) by physicochemical and spectroscopic methods. 3-Oxo-ursolic acid (3) was synthesized from ursolic acid (2) by Jones method. The cytotoxic activities of these compounds were tested against murine L1210 and human cancer cell lines (A549, SK-OV-3, SK-MEL-2, XF498, and HCT15) in vitro. Compounds 1 and 2 showed moderate cytotoxicities against L1210, whereas they showed weak activities against human cancer cell lines. However, compound 3 exhibited potent cytotoxic activities both in murine and in human cancer cell lines.     

大叶紫珠的化学成分

目的对大叶紫珠的化学成分进行研究。方法利用硅胶、Sephadex LH 20柱色谱分离和重结晶纯化,根据理化性质和NMR波谱数据进行结构鉴定。结果分离并鉴定了6个化合物,分别为α香树脂醇(αamyrin,1)、乌苏酸(ursolic acid,2)、2α,3α,19α三羟基12烯28乌苏酸(2α,3α,19αtrihydroxyurs 12 en 28 oic acid,3)、桦木酸(betulinic acid,4)、β谷甾醇(βsitosterol,5)、胡萝卜苷(daucosterol,6)。结论化合物1、3为首次从该植物中分离得到。