冬虫夏草对白血病NK细胞影响的体外研究

为探讨冬虫夏草（以下简称虫草）对白血病的作用及抑癌机制,观察了虫草水提液对正常人及白血病患者外周血NK细胞的影响,发现虫草可提高正常人及白血病NK细胞活性,在所使用的剂量范围内（1.56～25mg/ml）呈剂量-效应依赖性（r=0.984,P<0.01;r=0.988,P<0.01）。同时发现虫草可促进淋巴细胞表面CD₁₆抗原的表达,提高与K₅₆₂细胞的结合率。毒性试验表明对血细胞无毒性作用。认为虫草作为生物反应调节剂用于急性白血病治疗以提高NK细胞活性,值得进一步研究、开发与利用。     

Changes in responses of parafascicular nuclei during adjuvant-induced acute arthritis and effect of acupuncture in the rat

We have previously demonstrated that during acute arthritis in the rats induced by local intraplanter adjuvant, there are dramatic alterations in the behavioral responses to noxious stimulation. The purpose of this study was to observe the changes in responses of parafascicular nuclei (Pf) neurons during acute arthritis and effect of acupuncture (EA) in the rats. It was shown that: (1). for the whole populations of somatosensory neurons there was no statistical significant between arthritic rat and normal rat (P greater than 0.05). However, nociceptive neurons activated exclusively by noxious stimulation were less in the arthritic rat (21/60) than in the normal rat (35/46) (P less than 0.01); nociceptive-non-nociceptive neurons activated by both noxious and non-noxious stimulation were more in the arthritic rat (30/60) than in the normal rat (9/46) (P less than 0.01), and there was no statistical significant different for population of non-nociceptive neurons between two groups. (2). Nociceptive responses of Pf neurons (n = 16) could be markedly inhibited by EA at Xuanzhong (G39) and Kunlun (B60) in acute arthritic rat. In particular, the inhibitory effect was of great significance (P less than 0.01) during 0-10 and 20-35 minutes following cessation of EA. These results suggest that rats were hyperalgesic during adjuvant-induced acute arthritis and EA produces a fine analgesic effect in acute arthritic rats. The results also support the view that adjuvant-induced acute arthritic rat can be considered as a model for research of pain and analgesia.     

针刺对应激状态下小鼠细胞免疫功能影响的实验研究

目的 :揭示任脉六穴与胸腺的内在联系 ,探讨针灸对应激状态下小鼠细胞免疫功能的调节作用及机理。方法 :通过长期的捆绑刺激使小鼠处于应激状态 ,分别采用手针和电针刺激小鼠的任脉六穴 ,观察针刺前后NK细胞活性、白细胞介素 2活性的变化。结果 :应激组中NK细胞活性和白细胞介素 2活性均低于对照组 (P <0 .0 5,P <0 .0 1 ) ,而手针组、电针组与应激组相比NK细胞的活性和白细胞介素 2活性均得到提高 (P <0 .0 1 ,P <0 .0 5)。结论 :针刺任脉六穴可以提高应激状态下小鼠的细胞免疫功能 ,其作用机制主要是通过对胸腺的影响 ,进而提高小鼠的NK细胞活性和白细胞介素 2的活性 ,从而显现出针刺的促防卫免疫效应     

益气补肾颗粒治疗对微小残留白血病患者CD4~+CD8~(high) T淋巴细胞和自然杀伤细胞的影响

目的探讨益气补肾颗粒治疗后CD4+CD8highT淋巴细胞的变化及其对微小残留白血病(mini-mal residual leukemia,MRL)预后的意义。方法用多参数流式细胞技术比较分析36例MRL患者应用益气补肾颗粒治疗前后CD4+CD8 highT淋巴细胞亚群和自然杀伤细胞(NK细胞)改变。结果 (1)MRL患者3年持续完全缓解率、生存率分别为75.0%、80.5%,5年持续完全缓解率、生存率分别为63.9%、72.2%。(2)与治疗前比较,治疗后的MRL的CD4+CD8highT淋巴细胞百分率(%)和绝对值显著上升(P0.01);(3)与治疗前比较,治疗后MRL的NK细胞百分率(%)和绝对值均显著上升(P0.05,P0.01)。结论益气补肾颗粒可能通过调节机体自身免疫功能,提高CD4+CD8highT和NK细胞的绝对数量,杀伤或抑制体内残存白血病细胞,延长MRL患者的无病生存期。     

参芪扶正注射液配合化疗治疗急性白血病疗效及对T淋巴细胞亚群和血清IFN-γ、IL-10、IL-2水平的影响

目的 观察参芪扶正注射液配合化疗治疗急性白血病患者疗效及对T淋巴细胞亚群(CD4、CD8、CD4/CD8)和血清干扰素-γ(IFN-γ)、白细胞介素(IL)-10、IL-2的影响。方法 将65例初治白血病患者随机分为参芪扶正注射液加化疗组(参芪组,32例)及单纯化疗组(对照组,33例),观察其缓解率、治疗前后外周血成熟中性粒细胞绝对值的变化、T淋巴细胞亚群和血清IFN-γ、IL-10、IL-2水平。结果治疗后参芪组缓解率与对照组比较,差异无显著性(P>0．05)。化疗后第1、2、3周末两组外周血成熟中性粒细胞绝对值均降低,与本组化疗前比较,差异均有显著性(P<0．01或P<0．05);第3、4周末两组成熟中性粒细胞绝对值较化疗后第1、2周回升,且参芪组高于对照组,两组比较差异有显著性(P<0．05)。治疗后两组CD4、CD4／CD8均明显提高,与治疗前比较,差异有显著性(P<0．05,P<0．01)。治疗后两组血清IFN-γ、IL-2水平明显升高,血清IL-10水平明显下降,与治疗前比较,差异均有显著性(P<0．01);两组治疗后比较,差异亦有显著性(P<0．05)。结论参芪扶正注射液能改善、调节接受化疗的急性白血病患者的免疫功能,促进化疗后骨髓造血细胞增生,增强疗效。     

莪芪抗瘤方剂诱导白血病HL-60细胞凋亡的血清药理学研究

目的研究中药复方莪芪抗瘤方剂在体外对白血病HL-60细胞凋亡的作用,并探讨凋亡发生与细胞内Ca2 、Caspase-3、Bcl-2的关系。方法通过血清药理学方法,选择10%浓度的含药血清与白血病HL-60细胞共同培育24、48、72、96h后,应用荧光显微镜观察细胞形态,流式细胞仪检测凋亡峰(亚二倍体峰),用Fura-2荧光负载方法测定细胞内Ca2 浓度的变化。比色法检测凋亡相关基因Caspase-3活性变化。亲和免疫组化LSAB法检测HL-60细胞Bcl-2基因表达。结果10%浓度的含药血清处理组细胞体积缩小,细胞核固缩,膜起泡,核断裂及形成凋亡小体等,以72h最为明显;可见DNA含量低于G1期的凋亡细胞群,这群细胞的凋亡百分率随时间的递增而增加。10%浓度的含药血清处理组的细胞内Ca2 浓度也明显高于对照组(P<0.01),Caspase-3浓度明显高于对照组(P<0.01),Bcl-2基因表达明显低于对照组(P<0.05)。结论莪芪抗瘤方剂含药血清诱导白血病HL-60细胞发生凋亡,这种变化可能与细胞内Ca2 、Caspase-3水平上调及Bcl-2表达下调有关。     

Inflammatory responses of the rat lungs in cold-dryness syndrome in the northwest of China

[OBJECTIVE]:To examine changes in body weight and the lung inflammation factors interleukin-1β (IL-3),interleukin-8 (IL-8),IL-10 and tumor necrosis factor-α (TNF-c) in a rat model of cold-dryness syndrome in Northwest (Xinjiang) China to provide a reference for treating chronic obstructive pulmonary disease (COPD) with local peculiarities.[METHODS]:The rat COPD model was established by intratracheal instillation of porcine pancreatic elastase (PPE) in combination with cigarette smoking (CS).The rat model of cold-dryness syndrome of COPD in the northwest of China was set up by intratracheal instillation of PPE in combination with CS and environmental cold-dryness stress.The level of IL-3,IL-8,IL-10 and TNF-a in Bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA).The data were analyzed using the software SPSS 11.5.[RESULTS]:1) Body weight was less in the two model groups than that of control group(P＜0.01),PPE plus CS cold-dryness group was less than that of PPE plus CS group (P＜0.01).2) IL-13 in BALF significantly increased in PPE plus CS and cold-dryness group than that of control group (P＜0.01).3) IL-8and TNF-α in BALF significantly increased in PPE plus CS and cold-dryness group and PPE plus CS group than that of control group (,P＜0.01).[CONCLUSION]:Body weight in COPD model rats was reduced compared with controls.Cold-dryness may aggravate such a condition Lung inflammation in the model was mainly manifested by an increase in IL-13,IL-8 and TNF-α levels,with no change in IL-10 levels.Cold-dryness may aggravate lung inflammation of COPD.     

右归饮,四君子汤,桃红四物汤调节肾虚,脾虚,血瘀证患？…

目的：探讨右归饮、四君子汤、桃红四物汤对肾虚、脾虚、血瘀证患者免疫调节的不同作用。方法：用用右归饮、四君子汤、桃红四物汤分别治疗肾虚、脾虚、血瘀证患者各４０例,另分别设备证型２０例子安慰剂作对照,观察治疗前后免疫功能情况。结果：四君子汤使脾虚患者,右归饮使肾虚患者的循环免疫复合物（ＣＩＣ）水平下降,淋巴细胞转化主（淋转率）和自然杀伤（ＮＫ）细胞活性提高,四君子汤还使脾虚２的ＩＬ－２水平和嗜中性细胞     

CLINICAL STUDY ON ACUPUNCTURE TREATMENT OF SIDE REACTIONS OF RADIOTHERAPY AND CHEMOTHERAPY FOR MALIGNANT TUMOR

Radiotherapyandchemotherapyareimpor-tantmeasuresfortreatmentofcancer,buttheirdefectsofalsokillingthenormaltissuesandsidereactionsoftenaffectthetherapeuticeffectandthetherapeuticduration.Inthepresentpaper,radiotherapyandchemotherapycombinedwithacupuncturet…     

“吴氏消瘤散”抗S180实体瘤及免疫调节作用的实验研究

目的研究吴氏消瘤散对荷瘤S180小鼠肿瘤生长的影响,并观察其免疫调节作用。方法①S180移植瘤模型小鼠随机分为溶剂对照组(n=20)、环磷酰胺组(n=10)及吴氏消瘤散低、中、高剂量组(每组n=10),分别给予相应的干预措施,末次给药后24 h测定各组瘤重及抑瘤率。②将C57BL/6小鼠随机分为溶剂对照组(n=16)、香菇多糖组(n=8)及吴氏消瘤散低、中、高剂量组(每组n=8),分别给予相应的干预措施。以3H-TdR掺入法测定药物对脾T淋巴细胞增殖及NK细胞活性的影响。结果①与溶剂对照组比较,吴氏消瘤散各组小鼠瘤重明显降低,差异具有统计学意义(P0.01)。②与溶剂对照组比较,吴氏消瘤散各组小鼠能显著增强刀豆蛋白诱导的T淋巴细胞增殖,差异具有统计学意义(P0.01)。与YAC-1细胞共同培养后,吴氏消瘤散组NK细胞活性明显升高,与溶剂对照组比较差异具有统计学意义(P0.01)。结论吴氏消瘤散具有抗肿瘤作用,并可促进小鼠脾T淋巴细胞增殖,增强NK细胞活性。     

Salvia miltiorrhizae in the treatment of the viral myocarditis]

In Order to evaluate the effect of Salvia miltiorrhizae (SM) on the acute viral myocarditis (AVM), 60 children with AVM were studied. The patients were divided in random into two groups, group 1 treated with vit. C, ATP, CoA (n = 30), group II with SM plus vit. C, ATP, CoA (n = 30). The levels of plasma lipid peroxide (LPO), erythrocyte membrane microviscosity (EMMV), LDH, CPK, GOT and ECG in each patient were determined before and after one course of treatments respectively. The results revealed that before treatment the levels of plasma LPO and EMMV in both groups increased significantly compared with those of normal controls (n = 30, P < 0.01) respectively. There was a close correlation between LPO and EMMV (r = 0.6774, P < 0.01) and a close correlation between LPO and LDH (r = 0.5703, P < 0.01). After one course, the levels of plasma LPO and EMMV in both groups decreased significantly (compared with those before treatment, P < 0.01, respectively). But the LPO level and EMMV in group I were much higher than those in normal controls yet (P < 0.05, respectively). And LDH, GOT and ECG in nearly half of the patients in group I did not recover after one course while most patients in group II recovered. The results suggested that free radical plays an important role in the pathogenesis of AVM. SM as a good antioxidant, could protect myocardium from repairing membrane damage and clearing away free radical. This provided a new approach to treatment of viral myocarditis.     

人参总皂甙增加白血病细胞对化疗药物的敏感性

目的：探讨人参总皂甙（ＴＳＰＧ）对急性髓细胞白血病（ＡＭＬ）化疗药物敏感性的作用。方法：采用白血病祖细胞集落形成（ＣＦＵ－ＡＭＬ）药敏试验法。选用４种化疗药物：高三尖杉酯碱（ＨＨｒ）、阿糖胞苷（Ａｒａ）、阿霉素（Ａｄｒ）和足叶乙甙（Ｖｐ－１６）。结果：ＴＳＰＧ刺激ＣＦＵ－ＡＭＬ在体外增殖，集落数提高３７．９８％，使化疗药物对ＣＦＵ－ＡＭＬ的抑制率从原先的３０．４％￣４７．４％分别提高到５１．２％￣     

益气养阴清热法辅助治疗急性髓系白血病临床疗效观察

目的 探讨益气养阴清热法与化疗结合治疗急性髓系白血病的临床疗效。方法 114例患者随机分为治疗组68例和对照组46例。治疗组中医药以益气养阴清热法为基本治法。诱导缓解以DA、HA、MA、IA等联合化疗方案及急性早幼粒细胞白血病(M3)患者应用维甲酸、亚砷酸治疗。对照组仅用上述西药治疗。结果 治疗组完全缓解49例(72．1％),部分缓解9例(13．2％),总缓解率85．3％,与对照组比较,差异有显著性(P<0．01)。两组治疗后血象及骨髓像均有明显改善,血红蛋白及血小板的升高及骨髓白血病细胞数的降低治疗组优于对照组(P<0．05或P<0．01);两组治疗后免疫功能均有提高,CD4、CD4／CD8比值的升高治疗组优于对照组(均P<0．05);NK细胞活性的提高治疗组优于对照组(P<0．01)。结论 益气养阴清热法辅助治疗急性髓系白血病提高了临床疗效,有比较高的实用价值。     

冬虫夏草防治氨基糖甙肾毒性损伤的实验研究

为阐明冬虫夏草（简称虫草）对氨基糖甙肾毒性损伤的防治作用，我们进行了一系列的实验研究，结果表明：在庆大霉素急性肾损伤模型中，接受虫草治疗的大鼠尿ＮＡＧ酶、血肌酐水平低于对照组，肾小球滤过和保钠功能优于对照组。离体肾灌注（ＩＰＫ）研究证明，虫草可提高ＩＰＫ代谢率，增加肾小球滤过，保护肾小管正常运转。此外虫草还可减轻体外培养的肾小管细胞对庆大霉素损伤的易感性。虫草作用的机理可能包括：（１）拮抗氨基糖甙     

矫形药枕治疗颈椎病临床研究

为观察矫形药枕治疗各型颈椎病的疗效,将200例颈椎病按来诊顺序随机分为两组,以针灸治疗为对照组,以针灸加药枕矫形治疗为观察组.结果显示两组资料经统计学处理,总有效率及治愈率均有明显差异(P＜0.01),具有非常显著意义.表明矫形药枕治疗颈椎病有确切疗效.     

桂附胶囊治疗子宫内膜异位症实验研究

目的探讨桂附胶囊对实验性子宫内膜异位症大鼠激素及相关因素的影响.方法以子宫内膜自体移植法建立大鼠子宫内膜异位症模型.随机分为桂附胶囊组及丹那唑组.放射免疫法测定血清雌二醇(E2)、孕酮(P);免疫组化法测定雌激素受体(ER)、孕激素受体(PR)、血管内皮生长因子(VEGF)、NK细胞.结果桂附胶囊能够降低子宫内膜异位症大鼠外周血中E2、P水平(P＜0.01);降低异位内膜ER、PR的含量(P＜0.05);提高NK细胞活性(P＜0.05);并可抑制血管内皮生长因子(VEGF)的生成.桂附胶囊的实验效果与对照药丹那唑相近.结论桂附胶囊能够通过调节内异症大鼠的内分泌而抑制异位内膜的血管形成,从而抑制异位内膜的生长;同时增加NK细胞的活性,以加强对异位内膜组织的清除作用.     

Study on the granule of shencao fuzheng kangai]

The clinical effect of the granule of Shencao Fuzheng Kangai had been proved and the animal experiment was carried out. The results showed that: (1) No toxic response was found in acute toxicity test. (2) The granule could prevent WBC from decreasing severely in chemotherapy experiment (P less than 0.01). (3) It was indicated that the granule could improve the phagocytic function of macrophage in carbon clearance experiment (P less than 0.01). (4) It was meant that the granule could inhibit the growing of some solid carcinoma in inoculation experiments.     

不同中药注射剂对2型糖尿病并发急性脑梗死应激激素 …

目的：探讨不同中药注射剂对２型糖尿病并发急性脑梗死患者应激激素和免疫细胞因子的影响及其临床意义。和放射免疫分析法（ＲＩＡ）对使用蛇毒制剂（降纤酶）、刺五加及金钠多（银杏叶提取物）注射液３组病例（各为３２、２０、１２例）进行治疗前后促肾上腺皮质释放激素（ＣＲＨ）、促肾上腺皮质激素（ＡＣＴＨ）、皮质醇（ＣＳ）、肿瘤细胞坏死因子（ＴＮＦα）与白细胞介素－６（ＩＬ－６）含量检测。分析其变化特点,并与健康人     

脑心通对阿司匹林抗血小板治疗的干预作用

目的 探讨小剂量阿司匹林对心脑血管疾病患者血小板生化指标的影响及脑心通的干预作用。 方法 145例心脑血管疾病(糖尿病、高血压病、冠心病和脑梗死)患者服用阿司匹林1周后,检测用花生四烯酸(AA)和二磷酸腺苷(ADP)作诱导剂的血小板聚集率(PAG)、血浆P 选择素和血栓素B2(TXB2)浓度;然后随机分为阿司匹林组和脑心通组,均继续口服阿司匹林,脑心通组加用脑心通胶囊;治疗1个月后复查PAG、血浆P 选择素及TXB2浓度。 结果 第1次检测：血浆P-选择素、TXB2浓度与AA诱导的PAG均呈正相关(r=0.449, r=0.576,P<0.01),与ADP诱导的PAG均呈正相关(r=0.525, r=0.501,P<0.01);血浆P-选择素浓度与TXB2浓度呈正相关(r=0.610, P<0.01);两组间比较3项指标差异无统计学意义(P>0.05)。第2次检测：两组AA和ADP诱导的PAG、血浆P-选择素和TXB2浓度均显著低于第1次检测值(P<0.01);脑心通组上述指标均显著低于阿司匹林组(P<0.05);两组不良反应发生率差异无统计学意义(P>0.05)。 结论 心脑血管疾病患者服用阿司匹林1周时抗血小板疗效尚不理想,加用脑心通能提高其抗血小板疗效,且不增加不良反应。