HOW TO ENHANCE THE THERAPEUTIC EFFICACY OF AURICULO-POINT SEED PRESSING METHOD IN THE TREATMENT OF CHOLELITHIASIS

Chen Gongsun 陈巩荪(Department ofAcupuncture and Moxibustion,Jiangsu Pro-vincial People's Hospital)The first report on treatment ofcholelithiasis by Semen Vaccariaeauriculo-point pressing method was madeand published in 1977 by Jiangsu Provincial     

DIOXIN RECEPTOR GENE EXPRESSION IN DIFFERENT STAINS OF MICE

The aim of the present experiment is to find out whether there exist any differences in tissue expression of aryl     

NANOPARTICLE AS A NEW GENE TRANSFERRING VECTOR IN SPECIFIC EXPRESSION GENE

To evaluate the possibility and efficiency of nanoparticle as a new vector in specific gene transference.Methods. Nanoparticle-DNA complex was prepared with Poly- eM-lactic-co-glycolic acid (PLGA) bearing anti-sense monocyte chemotactic protein-1 (A-MCP-1), a specific expression gene, and the package efficiency, release progress in vitro, and the size of the complex were determined. The possibility of the new vector was evaluated with genomic DNA PCR by transferring gene into cultured smooth muscle cells (SMC), cationic lipids as a control. For study in vivo, jugular vein-to-artery bypass grafting procedures were performed on 20 New Zealand white rabbits, of which 6 grafts were transferred with nanoparticle-A-MCP-1 (200 μg), 6 with A - MCP - 1 (200 μ g) by cationic liposome, 4 with LNCX plasmid, and 4 as control. Fourteen days after the grafts were harvested, the expression of A-MCP-1 and its effect on MCP-1 in vein grafts were detected by dot blot, and the morphologic evaluation of grafts was performe     

THE APPLICATION OF A NOVEL THROMBIN SENSITIVE SITE IN GENETIC ENGINEERING

To obtain high efficiency of cleavage of thrombin in fusion protein containing α-ANP fused to Ref peptides,the linker sequence designed as VIAGR which was different from GVRGPR formerly used was studied, Plasmid pilL carrying the fusion gene Ref-NT-ANP downstream from Pc promoter was derived from expression vector pLY1 by inserting the fragment of NT-ANP into it. The expression of fusion gene was induced at 42℃,and the interested protein Ref-NT-ANP accumulated as inclusion bodies was isolated by gradient centrifuge and then dissolved in 7 mol/L guanidinehydrochloride(Gdn-HCl). After dilutlon,renaturation and dlalyzatlon,the cleavage of thrombln was examined using samples with 1.1 mol/L Gdn-HC1and samples free of Gdn-HC1 respectively. Digestion result showed that the novel-adopted cleavage scquence was highly sensitive to thrombin when the substrate dissolved in 1.1 mol/L Gdn-HC1.The time needed for 87% cleavage(the ratio of substrate to thrombin was 50 μg/u)was less than 24 hours. This sequence described here which was specifically recognized hy thrombin might be broadly applied in other fusion systems.     

EFFECT OF RADIX SALVIAE MILTIORRHIZAE ON THE GENE EXPRESSION OF NITRIC OXIDE SYNTHASE IN ISCHEMIC RAT BRAINS

The effect of Radix Salviae Miltiorrhizae (RSM) on the gene expression of nitric oxide synthase (NOS) in rat brains daring ischemia was studied with in situ hybridization and the results were analyzed with IBAS 2000 Image Analysis System. It was found that NOS gene expression of cerebral cortex and caudate-putamen was markedly increased in 24 hours in ischemia group (P< 0.01). In RSM-treated rats, although the NOS gene expression of ischemic side was also increased as compared with contralateral cortex and caudate-putamen, it was significantly lower in RSM-treated rats than those of the controls (P< 0.05, P< 0.01). The present study indicates that RSM can partly inhibit NOS gene ex pression of cerebral cortex and caudate-putamen during ischemia. This may be one of the protective mechanisms of RSM on cerebral ischemia.     

THE RELATIONSHIP BETWEEN mdrl GENE EXPRESSION AND DRUG-RESISTANCE IN OVARIAN CARCINOMA

INTRODUCTIONChemotherapyplaysamajorroleinthetreatmentofmalignantdiseases.Resultsoftreatmentwithanticanceragentshavesteadilyimprovedovertheyearsfollowingtheintroductionofmoreeffectivedrugsandtheestablishmentofbetterdesignedchemotherapystrategies.However,chemotherapyfailureduetocellulardrugresistanceremainsamajorprobleminmostcancerpatients.Manyfactorsaffecttheresponsetoanticancerdrugs.Thepresenceand/ordevelopmentofdrug--resistanttumorcellsareamongthemostimportantfactorsthatlimittheefficienc…     

TWO NOVEL MUTATIONS IN PHENYLALANINE HYDROXYLASE GENE AND IN VITRO EXPRESSION ANALYSIS ON MUTATION ARG252GLN

INTRoDUCTIoNPhenylketonuria(PKU)isanautosomalrecessivediseasecausedbythedeficiencyofaliver-specificen-zyme,phenylalaninehydroxylase(PAH).Itisoneofthemostcommoninbornerrorsofmetabolismwithaprevalenceof1in17OOOnewbornChinese(l).Recent-ly,theuseofalternativemolecularapproachesforstudy-ingPKUpatientsfromvariousethnicgroupsworldwidehasrevealedatleast14OdifferentpointmutationsinthehumanPAHgene(2,3),andmorethanl3mutationshavebeenreportedinChinesepopulations(4～9)'Tofurtherinvestigatethemol…     

EXPRESSION OF THE O~6－METHYLGUANINE－DNA METHYLTRANSFERASE GENE IN EIGHT HUMAN TUMOR CELL LINES

ＥＸＰＲＥＳＳＩＯＮＯＦＴＨＥＯ~６－ＭＥＴＨＹＬＧＵＡＮＩＮＥ－ＤＮＡＭＥＴＨＹＬＴＲＡＮＳＦＥＲＡＳＥＧＥＮＥＩＮＥＩＧＨＴＨＵＭＡＮＴＵＭＯＲＣＥＬＬＬＩＮＥＳＣｈｅｎＪｉａｎｍｉｎ；（陈建敏），ＺｈａｎｇＹａｎｐｅｉ；（章扬培）ａｎｄＷｕＹｉ?..     

THE ASSOCIATION OF POLYMORPHISMS AT A VNTR LOCUS 3’ TO THE APOLIPOPROTEIN B GENE WITH CORONARY HEART DISEASE IN CHINESE POPUL

ＴＨＥＡＳＳＯＣＩＡＴＩＯＮＯＦＰＯＬＹＭＯＲＰＨＩＳＭＳＡＴＡＮＴＲＬＯＣＵＳ３’ＴＯＴＨＥＡＰＯＬＩＰＯＰＲＯＴＥＩＮＢＧＥＮＥＷＩＴＨＣＯＲＯＮＡＲＹＨＥＡＲＴＤＩＳＥＡＳＥＩＮＣＨＩＮＥＳＥＰＯＰＵＬＡＴＩＯＮＹｅＰｉｎｇ（叶平），ＣｈｅｎＢ...     

PREDOMINANT EXPRESSION OF HUMAN Aγ-INCONTRAST WITH β-GLOBIN GENE IN MEL CELLS TRANSFECTED WITH THE CONSTRUCT μLCRAγψβδβ

A cosmid construct μLCRAγψβδβ were induced into mouse erythroleukemia cell lines 585 that expresses murine adult globin only and MEL GM-979 that expresses both murine embryonic and adult globins.Similar patterns of human globin gene expression were displayed in the two MEL cell lines transfected with the construct.Inducible expression of the Aγ-and β-gene was observed during induced cell differentiation.However,the expression level of the Aγ-globin gene is much higher than that of the β-globin gene in either uninduced or induced MEL transformants.No γ to β switching happened in the stable MEL transformants following a continuous culture.The much more effective enhance of the μLCR on the Aγ-globin gene than that on the β-globin gene is resulted probably from the fact that the distance between the LCR and the β-globin gene is much longer than that between the LCR and the Aγ-globin gene in the construct,in comparison with other constructs containing HS2 or μLCR linked to both of γ- and β-globin genes in different order.Two suggestions can be derived from these results:1) A competition between the γ- and β-globin gene for interaction with the LCR may indeed present,but only an enough long distance difference between the LCR to the γ-and to the β-gene can effectively influence the competition;2) Unlike transgenic mice,MEL cells are incapable of reconstructing the regulatory information involved in developmental control when it is provided by a fragment of the β-globin gene cluster with limited length.     

EXPRESSION OF ACTIVIN DURING THE MANDIBULAR DISTRACTION OSTEOGENESIS IN RABBIT

Objective To investigate the role of activin on osteogenesis during mandibular distraction.Methods Rabbit mandibular distraction model was used and the new regenerating tissue in the distraction zone were harvested at different time points, lmmunohistochemical technique for activin A was performed in the harvested tissues. Results Positive stain was noted in early phases of distraction. At the end of distraction phase osteoblasts and osteoid in primary mineralization front were strongly stained and osteoblasts and osteocytes in peripheral new bone zone were moderately stained. There were also broad activin A stains in osteoblasts and active osteocytes in early consolidation phase. Conclusion The expression of activin is increased during mandibular distraction. It could play an important role in the process of osteoblastic cells secretion, differen-tiation to osteocytes and bone formation during mandibular distraction.     

THE ANALYSIS OF NF2 GENE MUTATION IN SPORADIC SCHWANNOMAS

Ｒ啨ｓｕｍ啨　　 Ｏｂｊｅｃｔｉｆ　Ａｎａｌｙｓｅｒｌａｍｕｔａｔｉｏｎｄｕｇ埁ｎｅＮＦ2 (ｅｘｔｒｏｎ 2 ,4,6ｅｔ 13)ｃｈｅｚｌｅｓｓｃｈｗａｎｎｏｍｅｓ.Ｍ啨ｔｈｏｄｅｓ　Ｌａｍｕｔａｔｉｏｎｄｕ ｇ埁ｎｅＮＦ2ｃｈｅｚ 36ｓｃｈｗａｎｎｏｍｅｓ啨ｔａｉｔｏｂｓｅｒｖ啨ｅ ｐａｒＰＣＲ ＳＳＣＰｅｔｌ ａｎａｌｙｓｅｓ啨ｑｕｅｎｔｉｅｌｌｅｄｅｌ ＡＮＤ .Ｌ ｉｎｄｉｃｅｄｅ ｐｒｏｌｉｆ啨ｒａｔｉｏｎｄｕｓｃｈｗａｎｎｏｍｅ啨ｔａｉｔｄ啨ｔｅｃｔ啨 ｐａｒｌ ｉｍｍｕｎｏｈｉｓｔｏｃｈｉｍｉｅ .Ｒ啨ｓｕ…     

THE INFLUENCE OF APOLIPOPROTEIN B AND E GENE POLYMORPHISMS ON THE RESPONSE TO SIMVASTATIN THERAPY IN PATIENTS WITH HYPERLIPIDEMIA

Objective. To investigate the effect of apolipoprotein B (apoB) and E (apoE) genetic variations on lipid profile at baseline (before treatment), and also on the subsequent response to simvastatin therapy.Methods. Eighty-eight patients with hyperlipidemia were treated with simvastatin 5mg daily. The plasma levels of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C), triglyceride (TG) and apo B were measured pre-treatment and at the end of the 4th, 8th and 12th post-treatment week. Polymorphisms of apoB at XbaI locus and apoE were determined by restriction fragment length polymorphism (RFLP).Results. In all patients, relative frequencies of X- allele and X + allele were 0. 943 and 0. 057 for apoB gene respectively. For apoE gene the relative frequency of ε 2 allele was determined as 0. 182, ε3 as 0. 580 and ε4 as 0. 238. The reduction in TC level was more prominent in patients carrying X- allele than in those with X + allele following treatment (-23. 9% vs. -13. 6% , P < 0. 05). Co     

THE EFFECT OF ALL-TRANS RETINOIC ACID ON GAP JUNCTIONAL INTERCELLULARCOMMUNICATION AND CONNEXIN 43 GENE EXPRESSION IN GLIOMA CELLS

INTRODUCTIONGapjunctionsaresmallaqueouschannelsthatcon－nectneighboringcellsandallowthepassageofmoleculewhosemolecularweightisnotmorethan１０００Da．Invertebrates，gapjunctionsareformedbytheconjunctionoftwoconnexons，eachconsistingofahexamerofcon－nexinpr