5-羟色胺在肠易激综合征发病机制中的作用

肠易激综合征(irritablebowelsyndrome,IBS)是一种较为常见的慢性肠功能紊乱疾病,其发病率高,严重影响患者的生活质量,西方国家流行病学调查[1]显示,人群IBS发病率可高达15%;潘国宗等[2]调查显示,北京地区IBS的人群患病率为7.26%。IBS的病因目前尚不明确,发病机制复杂,病理表现主要与肠道动力异常、内脏高敏感性、炎症、脑肠轴和精神的异常等有关,也有学者认为其发病与神经-免疫-内分泌网络的异常密切相关。近年来,许多研究显示5-羟色胺(5-hydroxytryptamine,5-HT)在IBS的发病中有重要作用,而且5-HT及其受体的拮抗剂或激动剂被用于…     

心理障碍在肠易激综合征发病中的作用

目的　探讨心理障碍与肠易激综合征 (IBS)的相关性 ,应用百优解治疗IBS取得良效 ,进一步说明心理障碍在IBS发病中的作用。方法　选自我院门诊和住院的IBS患者 5 2例 ,经各项检查排除器质性疾病 ,均符合罗马ⅡIBS诊断标准。其中男 2 4例 ,女 2 8例 ,年龄 2 0～ 5 4岁 ,平均 34 8岁。对照组 35例 ,男 16例 ,女 19例。年龄18～ 5 1岁 ,平均 32 3岁。无消化道疾病及其他躯体病。两组资料的年龄、性别及受教育程度等均无显著差别 ,有可比价值。由专职医师对两组患者分别进行HAMD(汉密顿抑郁量表 )和SCL 90表测试评分。HAMD评分≥ 2 0…     

肠易激综合征发病机制的初步研究

肠易激综合征发病机制的初步研究第四军医大学西京医院全军消化病研究所（西安７１００３２），第一军医大学杨云生张振书宋于刚张万岱周殿元冯福才陈昱肠易激综合征（ｉｒｉｔａｂｌｅｂｏｗｅｌｓｙｎｄｒｏｍｅ，ＩＢＳ）是一种肠运动功能紊乱性疾病，发...     

2.79心理障碍在肠易激综合征发病中的作用

目的探讨心理障碍与肠易激综合征(IBS)的相关性,应用百优解治疗IBS取得良效,进一步说明心理障碍在IBS发病中的作用. 方法选自我院门诊和住院的IBS患者52例,经各项检查排除器质性疾病,均符合罗马ⅡIBS诊断标准.     

肠离子通道在肠易激综合征发病机制中的作用研究进展

离子通道广泛存在于消化道黏膜中,其表达和(或)功能异常是肠易激综合征(IBS)重要的致病机制之一。电压门控钠离子通道(VGSCs)、电压门控钙离子通道(VGCCs)及瞬时受体电位通道(TRPs)等离子通道不同程度地参与IBS胃肠运动及内脏超敏,是调节胃肠运动和内脏超敏的关键病理生理机制及治疗靶点。     

NLRP3炎症小体在神经血管疾病中的作用

炎症小体是多蛋白复合物,可以招募并诱导半胱氨酸天冬氨酸酶-1前体(pro-caspase-1)成为有活性的半胱氨酸天冬氨酸酶-1(caspase-1),从而促进IL-1β和IL-18成熟与释放。许多研究表明NLRP3炎症小体与缺血性脑卒中、阿兹海默症(AD)、外伤性脑损伤(TBI)、脑瘤等神经血管疾病的发生发展密切相关。本文综述了NLRP3炎症小体激活途径,并着重介绍了NLRP3炎症小体介导的在上述几种神经血管疾病中的作用,为相关研究提供了基础资料。     

老年肠易激综合征患者外周血T淋巴细胞亚群临床分析

目的： 探讨老年肠易激综合征患者外周血T淋巴细胞亚群与健康老年人相比是否存在差异，并分析其原因。方法： 对38名肠易激综合征患者及40名健康老年对照者用流式细胞仪检测外周血T淋巴细胞亚群。结果： 与健康对照者相比，肠易激综合征患者CD4+、CD4+/CD8+比例，NK均低于健康对照者，CD8+则高于健康对照者。结论： 与健康老年人相比，肠易激综合征患者外周血T淋巴细胞亚群存在异常，提示肠易激综合征与免疫异常、免疫紊乱有关。     

黄连素缓解肠易激综合征作用机制的研究进展

<正>肠易激综合征（irritable bowel syndrome,IBS）是由饮食、遗传、肠道感染等因素触发,以腹痛、腹胀、腹部不适、排便频率或大便性状改变为主要表现的一种中青年女性多发性肠道功能紊乱性疾病,患者肠道无器质性病变,常伴有乏力、消化不良、焦虑等症状。IBS的症状根据罗马Ⅳ标准可分为腹泻型（IBS-D）、便秘型（IBS-C）、混合型（IBS-M）及不定型（IBS-U）,其中IBS-D的发病率最高。流行病学显示,     

成纤维细胞转录BDNF基因后的条件培养液在体外培养中对神经突起生长的作用

将带有 ＢＤＮＦ基因的成纤维细胞（ＮＩＨ／３Ｔ３）（由上海生化所提供）冻存－７０℃, ６个月后将此工程细胞复苏,放在３７℃、５％ＣＯ_２培养箱中培养扩增,观察形态学并同时收集其培养液,对新生ＳＤ大鼠的脊神经节、脊髓和大脑皮层中的神经元进行条件培养并设对照组。脊神经节植块培养２天后显示ＢＤＮＦ转基因成纤维细胞的条件培养液对促进神经元突起的生长具有良好的活性。对脊髓、大脑皮层中神经元分别进行分散原代培养,１５天后,其突起长度作图像分析（Ｌｅｉｃａ Ｑ５００ ＩＷ）并分别计算出它们的平均长度。脊髓实验组为 １９２． ２０±７５μｍ,对照组为１６０．３７±４８μｍ。大脑皮质实验组为３４７．１４±１０６μｍ,对照组为１２５．６７±４２μｍ。两组的ｔ检验分别是Ｐ＜０．０５。结果显示ＢＤＮＦ转基因成纤维细胞的条件培养液对体外培养神经元突起的生长具有一定的促生长作用。     

The role of diet in the pathogenesis and management of irritable bowel syndrome (Review)

Most patients with irritable bowel syndrome (IBS) believe that diet plays a significant role in inducing IBS symptoms and desire to know what foods to avoid. It has been found that the intake of calories, carbohydrates, proteins and fat by IBS patients does not differ from that of the background population. IBS patients were found to avoid certain food items that are rich in fermentable oligo-, di- and monosacharides and polyols (FODMAPs), but they did have a high consumption of many other FODMAP-rich food items. The diet of IBS patients was found to consist of a low calcium, magnesium, phosphorus, vitamin?B2 and vitamin?A content. There is no consistent evidence that IBS patients suffer from food allergy, nor is there documented evidence that food intolerance plays a role in IBS symptoms. Abnormalities in gut hormones have been reported in IBS patients. As gut hormones control and regulate gastrointestinal motility and sensation, this may explain the abnormal gastrointestinal motility and visceral hypersensitivity reported in these patients. Guidance concerning food management which includes individually based restrictions of FODMAP-rich food items and individual evaluation of the effects of protein-, fat- and carbohydrate-rich/poor diets may reduce IBS symptoms.     

肠易激综合征患者肠黏膜肥大细胞在内脏高敏感机制中的可能作用和临床意义

目的:探讨肠易激综合征(IBS)患者肠黏膜肥大细胞(MC)对内脏敏感性改变的作用及其临床意义。方法:分别检测50例IBS患者和20例正常对照组患者的肛门直肠括约肌静息压、最大收缩压和括约肌松弛压,再分别取样各患者回肠末端、回盲部、升结肠和乙状结肠处的4块黏膜标本,采用甲苯胺蓝改良染色法进行MC染色并分析其密度。结果:IBS患者肛门直肠括约肌的静息压、最大收缩压和松弛压与正常对照组比较差异无统计学意义(P0.05);感觉阈值、疼痛阈值和排便阈值均显著低于正常对照组(P0.05);IBS组患者回肠末端、回盲部以及升结肠黏膜MC密度均显著高于正常对照组(P0.05);腹泻型IBS组患者回肠末端、回盲部以及升结肠黏膜MC密度均显著高于便秘型IBS组(P0.05)。结论:IBS患者肠黏膜MC存在显著异常,可能对IBS内脏高敏感性的形成具有重要作用。     

Prophylactic role of maternal administration of probiotics in the prevention of irritable bowel syndrome

Neonatal stress is a common early life event which alters the development of the endocrine and immune systems. Specifically, exposure to neonatal stress results in alterations to the hypothalamic–pituitary–adrenal (HPA) axis resulting in offspring who hyper-respond to stress in adulthood. Recently, this concept has been applied to the ontogeny of functional gastrointestinal (GI) disturbances such as irritable bowel syndrome (IBS). The high prevalence of this disorder and the ineffectiveness of current treatments results in high direct and indirect costs to the society. Recently, administration of probiotics to neonates has been used as a safe and cost-effective preventative strategy to revoke the long term unfavourable imprinting induced on the gastrointestinal system by early life stressors in animal models of human IBS. It is not as yet known however, whether maternal supplementary probiotics may also contribute to improved GI integrity and gut-associated immune functioning in stressed neonates, if these possible improvements persist into adulthood, or how this protective effect may be mediated. Our hypothesis is an attempt to link this proposed nutritional approach and its possible preventive effects against GI dysfunctions provoked by neonatal stress.     

肠易激综合征动物模型研究进展

肠易激综合征(IBS)是一种常见的肠功能紊乱性疾病,其特点是慢性反复发作的腹痛、腹部不适及排便习惯的改变。IBS的发病机制目前尚未阐明,研究结果显示IBS的发病可能与肠道动力、内脏感觉过敏和肠道感染等因素有关。现有的IBS动物模型根据其病理学机制主要分为二大类,一是以中枢为靶点的刺激(社会心理因素);二是以外周为靶点的刺激(肠道炎症、感染)造成的IBS动物模型。本文对IBS动物模型的研究现状及进展做一综述。     

Elevated circulating miR-150 and miR-342-3p in patients with irritable bowel syndrome

Background and aims

MicroRNAs (miRNAs) are small non-coding RNAs, which regulate gene expression and are thus of interest as diagnostic markers, and as clues to etiology and targets of intervention. This pilot study examined whether circulating miRNAs are differentially expressed in patients with IBS.

Methods

miRNA microarrays (NanoString) were run on the whole blood of 43 participants.

Results

hsa-miR-150 and hsa-miR-342-3p were found to be significantly elevated (FDR adjusted p ≤ 0.05, ≥ 1.6 fold change) in IBS patients compared to healthy controls. Neither of these miRNAs showed any relationship to race or sex. hsa-miR-150 is associated with inflammatory bowel disorders and pain, and interacts with a protein kinase (AKT2) through which it may affect inflammatory pathways. hsa-miR-342-3p is predicted to interact with mRNAs involved in pain signaling, colonic motility, and smooth muscle function.

Conclusions

This preliminary study reports the association of two miRNAs, detected in whole blood, with IBS. These miRNAs link to pain and inflammatory pathways both of which are thought to be dysregulated in IBS. Larger sample sizes are needed to confirm their importance and potential as biomarkers.     

The efficacy of group therapy for patients with irritable bowel syndrome

Twenty patients with irritable bowel syndrome underwent six weeks of group therapy incorporating behavioral and didactic techniques. Psychometric follow-up study revealed a significant reduction in dysphoric emotions despite the persistence of somatic complaints. These findings are evaluated in the context of the subjects’ cognitive styles of locus of control and field-dependence.