The Expression of Interleukin-22 and S100A7, A8, A9 mRNA in Patients with Psoriasis Vulgaris

In order to study the expression of interleukin-22 （IL-22） and S 100A7, A8, A9 mRNA in the skin lesions of patients with psoriasis vulgaris and their relationship, the biopsies were taken from skin lesions in 35 patients with psoriasis vulgaris and the skin of 16 normal controls, and the expression levels of 1L-22 and S 100A7, A8 and A9 mRNA were detected by semi-quantitative RT-PCR. The results showed that （1） IL-22 and S 100A8, A9 mRNA were positively expressed in the psoriatic skin lesions but negatively expressed in the normal controls; The expression level of S 100A7 was （1.133±0.040） in the psoriatic skin lesions, significantly higher than that in the normal controls （0.744±0.037, P〈0.01）. （2） There were significantly positive correlations between the expression of IL-22/S100A7 mRNA, IL-22/S100A8 mRNA, IL-22/S100A9 mRNA in the psoriasis vulgaris （r1=-0.543, r2=0.774, r3=0.621, P〈0.01）. It was concluded that IL-22 and S 100A7, A8, A9 might play important roles in the occurrence and progression of psoriasis.     

The Expression of Interleukin-17, Interferon-gamma, and Macrophage Inflammatory Protein-3 Alpha mRNA in Patients with Psoriasis Vulgaris

Interleukin 17(IL 17)isanewlyidentifiedpro inflammatorycytokinethatisproducedbyactivatedTcells.IL 17hastheabilitytoinducestromacellstoproduceproinflammatorycytokinesandhematopoieticcytokines .Soitmayparticipateinsomeinflammatoryreactionsandsomeimmunoloreg…     

Expression of LL-37, Human beta Defensin-2, and CCR6 mRNA in Patients with Psoriasis Vuigaris

Theinnateimmunesystemofhumanskincon tainsantimicrobial peptidesknownascathelicidins(LL 37)andhumanbeta defensins (hBD) [1] .Innor malskinthesepeptidesarenegligible ,buttheexpres sionofthemwillbetriggeredbyinjuryorinflamma tionoftheskin .Andtheirexpression…     

Expression of p63 and Cyclooxygenase-2 and Their Correlation in Skin Tumors

To study the expression of p63 and cyclooxygenase-2 (cox-2) in skin tumors and evalu- ate the correlation between p63 and cox-2, the expressions of cox-2 and p63 were measured by streptavidin-peroxidase complex immunohistochemical technique in 17 cases of skin squamous cell carcinoma(SCC), 19 cases of Bowen's disease(Bowen), 11 cases of actinic keratosis(AK), 12 cases of seborreic keratosis(SK) and 13 specimens of normal skin. Our results showed that the expression of p63 in skin squamous cell carcinoma, Bowen's disease and actinic keratosis were significantly higher than that in seborreic keratosis, while the expression of p63 in seborreic keratosis was sig- nificantly higher than that in normal skin. The expression of cox-2 in skin squamous cell carcinoma, Bowen's disease and actinic keratosis were significantly higher than that in seborreic keratosis, while no statistical difference was noted in the expression of cox-2 between seborreic keratosis and normal skin. Cox-2 expression was positively correlated with the high p63 expression in malignant skin tu- mors. The increased expression of cox-2 and p63 may play an important role in the development of skin tumors and work synergetically in malignant skin tumors.     

Expression of Interleukin-17 in Lung and Peripheral Blood of Asthmatic Rats and the Influence of Dexamethasone

The expression of interleukin-17(IL-17) in lung and peripheral blood of asthmatic rats and the influence of dexamethasone,and the role of IL-17 in the pathogenesis of asthma were investigated.Thirty Sprague-Dawley(SD) adult rats were randomly divided into three groups(n=10 in each group):normal group,asthmatic group,and dexamethasone-interfered group.Rat asthmatic model was established by intraperitoneal(i.p.) injection of 10% ovalbumin(OVA) and challenge with 1% OVA via inhalation.Rats in dexamethasone-interfered group were pretreated with dexamethasone(2 mg/kg,i.p.) 30 min before each challenge.The expression of IL-17 protein in serum and bronchoalveolar lavage fluid(BALF) was detected by ELISA.The expression of IL-17 mRNA in peripheral blood mononuclear cells(PBMC) and BALF cells was semi-quantitatively detected by RT-PCR.The expression of IL-17 protein in serum and BALF of asthmatic rats was significantly elevated as compared with normal rats and dexamethsone-interfered rats(P<0.01),and there was significant difference between normal rats and dexamethsone-interfered rats(P<0.05).The expression of IL-17 mRNA in PBMC and BALF cells of asthmatic rats was markedly increased as compared with normal rats and dexamethsone-interfered rats(P<0.01),and significant difference was found between normal rats and dexamethsone-interfered rats(P<0.05).It was concluded that the expression of IL-17 was increased significantly in asthmatic rats and could be inhibited partly by dexamethasone,suggesting that IL-17 might play an important role in the pathogenesis of asthma as an inflammation regulation factor.     

The Value of p63 and CK5/6 Expression in the Differential Diagnosis of Ductal Lesions of Breast

Differentiation between benign and malignant lesions has always a problem in the pathological diagnosis. Presence of myoepithelial cells (MECs) is an vital indi- cator for existence of cancer or invasion[1, 2]. Although these histological and morphological features have been well-depicted, it is still difficult to determine the pres- ence of MECs in breast lesions and to evaluate the nature of a lesion by routine HE staining under light microscope. So it is of importance to find useful mark…     

Expression of CC Chemokine Ligand 20 and CC Chemokine Receptor 6 mRNA in Patients with Psoriasis Vuigaris

Itiswell knownthatimmune inflammatorymechanism playsanimportantroleinpsoriasis .Therelationshipbetweenchemokine ,receptorsandpsori asishasbeenconfirmed[1] .CCchemokineligand 2 0(CCL2 0 )isanewmemberinthefamilyof β chemokineandalsotheonlyligandofCCchemoki…     

The Effect of Interleukin-18 on Airway Inflammation in Asthmatic Murine Models and Its Mechanisms

In order to investigate the effect of interleukin-18 （IL-18） on airway inflammation in asthmatic murine models and its mechanisms, BALB/C mice were randomly divided into three groups （n=10 in each group）： group A （control group）; group B （asthmatic model group）; group C （IL-18-treated group）. The asthmatic model was established in groups B and C by respiratory syncytial virus （RSV） killed by ultraviolet. Saline solution （0.1 mL） and IL-18 （0.1 mL, 1μg） were intraperitoneally injected respectively in groups B and C at 7 time points （day 1, 2, 7, 8, 9, 21, 22）. The number of eosinophils （EOS） and plasmacytes in the airway was observed. The levels of interferon gamma （IFN-γ） in bronchoalveolar lavage fluid （BALF） were measured by ELISA. The results showed that symptoms of asthma in group C were more severe than in groups A and B. In group A, there were no EOS and plasmacytes in the airway submucosa. The number of EOS [ 15±3 （average cell counts per microscopic visual field, the same below）] and plasmacytes （10±2） in group B were increased significantly. However, the number of EOS and plasmacytes in group C （6±2 and 2±1, respectively） was decreased significantly as compared with group B （both P〈0.05）. The levels of IFN-γ in groups A, B and C were 31±3, 40±5 and 63±5 μg/mL respectively, and those in group C were significantly higher than in groups A and B （both p〈0.05）. It was suggested that the mechanism by which IL-18 inhibited the airway inflammation in asthmatic mice might be contributed to the fact that IL-18 could induce the induction of IFN-γ.     

Expression of Angiopoietin-1/-2 in the Process of Mouse Embryo Implantation

This study examined the expression and distribution of angiopoietin-1/-2 (Ang-1/-2) in the endometrium of early pregnant mice. The expression of Ang-1/-2 was detected by immunohisto- chemical staining and in situ hybridization respectively. Computerized image analysis system was used to measure the average optical intensity of Ang-1/-2 in endometria at different time points after gestation. Mice were randomly divided into 5 groups: control group, D2 group (2 days after preg- nancy), D4 group (4 days after pregnancy), D6 group (6 days after pregnancy) and D8 group (8 days after pregnancy), each containing 15 mice. The results showed that the expression of Ang-1 and Ang-2 was very different among 4 groups (P<0.01). Immunohistochemical staining revealed that Ang-1 was localized in the cytoplasma of stromal cells 2 days after pregnancy (day 2), and in luminal epithelial cells on day 4. The protein of Ang-2 was mainly expressed in the cytoplasma of glandular epithelia and stromal cells. With gestation time, the positive reactions of Ang-1/-2 were stronger in the endometria of the pregnant mice (P<0.01). In situ hybridization showed Ang-1 mRNA in stromal cells on day 2. Hybridization signal was localized in both stromal cells and vessel epithelial cells on day 4; Ang-2 mRNA was expressed in stromal cells and glandular epithelia on day 2; high mRNA levels appeared in stromal cells, glandular epithelia and vascular endothelia on day 4; an increasing in mRNA expression of Ang-1/-2 was observed on day 6 and day 8 (P<0.01). It is suggested that Ang-1/-2 may play an important role in the cross-talk between blastocyst and maternal endometrium during the process of embryo implantation.     

猪白细胞介素12 p35、p40亚基基因的克隆和序列分析

目的:克隆猪白细胞介素(IL)-12p35、p40亚基基因,为制备猪囊尾蚴的复合基因疫苗奠定基础。方法:分别分离成年猪的外周血单个核细胞和脾细胞,培养10h后用IFN-γ(100ng/ml)刺激增殖12h,随后加入细菌脂多糖(1μg/ml)刺激培养3h,分别收集细胞并提取细胞总RNA,用RT-PCR方法扩增猪IL-12p35、p40cDNA编码基因,克隆至pMD18-T载体,经PCR和双酶切鉴定后进行序列测定。结果:猪IL-12p35、p40cDNAPCR产物电泳结果证明所克隆的基因分别为616bp和1011bp,基因测序结果与GenBank报道的基因序列各有一个碱基差异,但编码氨基酸无差异,证实分别为猪IL-12p35、p40cDNA编码基因。结论:成功克隆了猪IL-12p35、p40cDNA编码基因。     

胸腺五肽对复发性外阴阴道念珠菌病患者IL-12p70和IL-23p19水平的影响及疗效分析

目的研究观察胸腺五肽对复发性外阴阴道念珠菌病患者阴道分泌物IL-12p70和IL-23p19水平的影响及疗效。方法采用酶联免疫吸附实验（ELISA）双抗体夹心法检测复发性外阴阴道念珠菌病患者治疗组、对照组治疗前后及正常人阴道分泌物IL-12p70和IL-23p19水平。结果两组治疗前复发性外阴阴道念珠菌病患者IL-12p70和IL-23p19水平均低于正常人（P〈0.01）,两组患者间IL-12p70和IL-23p19水平相近（P〉0.05）。治疗后胸腺五肽组IL-12p70和IL-23p19水平升高,对照组治疗前后水平差异无统计学意义（P〉0.05）。治疗组的疗效（总有效率80.00%）明显高于对照组的43.33%（P〈0.05）。结论复发性外阴阴道念珠菌病患者存在着Th1/Th2型细胞因子失衡,胸腺五肽可通过纠正两型细胞因子失衡而改善患者免疫功能,提高治疗效果。     

雷公藤多苷对结肠炎小鼠结肠组织IL-23、IL-17和IL-12表达的影响

目的:观察雷公藤多苷( GTT)、美沙拉嗪对三硝基苯磺酸(TNBS)诱导的急性结肠炎小鼠结肠组织中IL-23、IL-17、IL-12表达的影响,探讨其可能的作用机制.方法:C57BL/6小鼠随机分为4组:正常对照组、模型组、美沙拉嗪组、雷公藤多苷组,后3组应用TNBS建立小鼠急性结肠炎模型,模型组不作其他处理,雷公藤多苷组和美沙拉嗪组于造模前4 d开始每天给予雷公藤多苷灌胃或美沙拉嗪灌肠液灌肠直到实验结束,正常对照组不作任何特殊处理.各组小鼠均于TNBS灌肠后48 h处死,检测各组小鼠结肠组织大体、组织学损伤评分及髓过氧化物酶(MPO)活性;ELISA法检测结肠组织IL-23 p19、IL-17蛋白含量,实时荧光定量RT-PCR检测结肠组织IL-23 p19、IL-17、IL-12 p35的mRNA表达.结果:美沙拉嗪组和雷公藤多苷组小鼠结肠大体及组织学损伤记分、MPO活性明显低于模型组(P<0.05);模型组结肠组织IL-23 p19、IL-17、IL-12 p35 mRNA表达水平明显高于正常对照组、美沙拉嗪组、雷公藤多苷组(P<0.05);模型组结肠组织IL-23 p19、IL-17蛋白水平明显高于正常对照组、美沙拉嗪组、雷公藤多苷组(P<0.05),后三者间无统计学差异.结论:雷公藤多苷可能通过非选择性抑制IL-23 p19、IL-17、IL-12 p35的表达来抑制结肠炎小鼠的炎症反应,效果与美沙拉嗪类似.     

IL-12p40、IL-12p70在经典型霍奇金淋巴瘤中的表达及意义

目的 探讨白细胞介素-12p40(IL-12p40)、白细胞介素-12p70(IL-12p70)在经典型霍奇金淋巴瘤(CHL)组织中的表达及意义. 方法 应用免疫组织化学法检测IL-12p40、IL-12p70在41 例CHL组织、92 例弥漫性大B细胞淋巴瘤(DLBCL)组织及20 例反应性淋巴组织增生(RLH)中的表达. 结果 IL-12p40、IL-12p70在41例CHL及92例DLBCL中的阳性表达率分别是 51. 22%、70. 73% 及 0. 00%、4. 35%,20 例 RLH 中未见 IL-12p40 及 IL-12p70 表达. IL-12p40、IL-12p70在CHL 中及各亚型中的表达均明显高于DLBCL及RLH(P<0. 05). IL-12p40及IL-12p70的表达与患者性别、年龄、肿块大小、B症状、临床分期、血清乳酸脱氢酶水平、国际预后评分等临床病理因素均无关. IL-12p40与IL-12p70在CHL中的表达有相关性(P<0. 05). 结论 IL-12p40、IL-12p70在CHL发病中有重要作用.     

2型糖尿病患者IL-23p19 mRNA及血浆IL-23水平的变化和意义

目的探讨2型糖尿病(T2DM)治疗前后外周血单个核细胞中IL-23p19 mRNA水平以及血浆中IL-23水平的变化。方法检测空腹血糖水平选定分组对象,采用逆转录-聚合酶链式反应(RT-PCR)法检测58例2型糖尿病患者(初诊糖尿病患者28例,经治疗患者30例)和26例健康体检者(对照组)全血中IL-23p19 mRNA的水平,同时采用酶联免疫吸附试验(ELISA)检测各组血浆中IL-23的水平。结果初诊糖尿病组IL-23 mRNA表达水平高于经治疗组和对照组,差异具有统计学意义(P<0.05),IL-23血浆水平比较初诊糖尿病组与经治疗组及对照组差异有统计学意义(P<0.05)。结论 2型糖尿病治疗前IL-23及p19 mRNA水平明显高于经治疗组和对照组,IL-23可能与2型糖尿病的发病和病程进展有一定的关系。     

人白细胞介素23 p19亚基基因的克隆和序列分析

目的:克隆人白细胞介素-23(IL-23)p19亚基基因。方法:分离健康成年人外周血单个核细胞,常规培养后用脂多糖至终浓度为100 ng/m l刺激增殖12 h,收集细胞并提取细胞总RNA,用RT-PCR方法扩增人IL-23 p19 cDNA编码基因,克隆至pMD18-T载体,PCR鉴定后进行序列测定。结果:RT-PCR产物电泳结果显示所扩增的基因为734 bp,基因测序显示其序列与GenBank报道的人IL-23 p19基因序列完全一致。结论:成功地克隆了人IL-23 p19 cDNA编码基因。     

IFN、IL-4、IL-12及IL-12P40在子宫内膜异位症的变化

目的 探讨盆腔内子宫内膜异位症(endometriosis,EM)患者IFN、IL-4、IL-12、以及IL-12P40细胞因子平衡与EM发病的相关性。方法 用半定量PCR法和ELISA酶联免疫检测20例EM患者的血及腹腔中的IFN、IL-4、IL-12及IL-12P40细胞因子,并与无子宫内膜异位症患者相比较,以观察上述指标在EM患者血及腹腔液中的变化。结果 1.EM患者血清和腹腔液中IL-12浓度分别为(66.38±12.6)pg/ml和(77.76±14.6)pg/ml,显著低于无EM对照组(84.97±13.7)pg/ml和(106.92±10.7)pg/ml(P均<0.05)。2.EM患者血清中IL-12P40(35.64±10.6)pg/ml,与对照组无显著性差别(P>0.05);其腹腔液中含量为(79.76±12.6)pg/ml,显著高于对照组(40.54±10.0)pg/ml(P<0.05),并与EM的严重程度呈负相关。3.EM组IL-4/IFN-γ比值0.328显著高于对照组(0.07)。结论 EM患者体内IL-12含量降低,IL-12P40含量增高,这可能与EM的发生密切相关的NK细胞功能下降有关。EM患者外周血单核淋巴细胞中IL-4细胞因子升高,在Th1/Th2细胞因子平衡网络中出现Th2细胞的偏移并占优势。     

阿维A联合NB-UVB与卡泊三醇治疗银屑病及对IL-l7、22、23的影响

目的 探讨应用阿维A联合NB-UVB与卡泊三醇治疗银屑病疗效及对银屑病患者外周血血清中IL-17、IL-22、IL-23水平的影响。方法 将108例患有重度的寻常型斑块状银屑病的病人,随机平均分成为A、B、C三组:其中A组病人治疗时采取窄谱中波紫外线(NB-UVB)全身照射的方法,每周3次;B组于每日早晚清洁患处皮损后外用卡泊三醇软膏;C组病人治疗时则联合应用窄谱中波紫外线(NB-UVB)光疗及卡泊三醇软膏的方法。参与实验的患者治疗时均口服阿维A胶囊,整个实验的治疗疗程为8周。观察三组患者的临床疗效并进行PASI评分以及DLQI评分比较。检测治疗前后患者外周血血清中IL-17、IL-22、IL-23表达的变化情况。结果 应用阿维A单独或同时联合 NB-UVB光疗及外用卡泊三醇软膏均能够使得患者的PASI评分、DLQI评分、外周血血清中的相关炎症因子IL-l7、IL-22和IL-23水平均较治疗前有明显降低,且口服阿维A同时联合外用卡泊三醇软膏和 NB-UVB光疗治疗重度寻常型斑块状银屑病能够达到更好、更显著的近期治疗效果,该组患者的PASI评分和DLQI评分较阿维A单独联合NB-UVB光疗或卡泊三醇软膏治疗降低更显著,有更加明显的治疗效果,患者外周血血清中相关炎症因子IL-l7、IL-22和IL-23水平降低较应用阿维A单独联合NB-UVB光疗或卡泊三醇软膏的治疗方案时更明显。此种治疗方案能够使得患者的近期治疗效果更理想,从而达到改善提高病人生活质量的目的。结论 阿维A联合NB-UVB光疗和卡泊三醇软膏治疗重度寻常型斑块状银屑病患者疗效更显著,可以作为治疗重度寻常型斑块状银屑病的常规治疗方案;阿维A联合NB-UVB光疗和卡泊三醇软膏治疗重度寻常型斑块状银屑病外周血血清中IL-17、IL-22和IL-23水平降低更明显;患者外周血血清中IL-17、IL-22、IL-23水平的变化作为实验室治疗效果的一种评价指标。     

A study on the expression of interleukin(IL)-10 and IL-12 P35, P40 mRNA in the psoriatic lesions

Psoriasisisa common chronic,relapsing and in-flammatory disease and its pathogenesis is still un-known.It is considered thatit is one kind of geneticand immunological diseases controlled by multiplegenes.Even though theexactpathogenesisisstillun-known,many studies showed that T cells mediatedimmune response played a very important role in thepathogenesis of psoriasis.In this study,the expres-sion of IL- 1 0 ,IL- 1 2 P35 and P40 m RNA in the pso-riasis lesions were detected by using RT- P…