急性加重期慢性阻塞性肺疾病患者血清C 反应蛋白及降钙素原水平变化及意义

目的：探讨急性加重期慢性阻塞性肺疾病（COPD）患者血清C反应蛋白（CRP）及降钙素原（PCT）的变化及临床意义。方法纳入急性加重期COPD患者120例,同时按随机数字表法抽取COPD稳定期患者120例,所有受试者均给予相应的对症支持治疗,观察治疗前、治疗后1 d、7 d、14 d患者血清CRP与PCT水平变化。结果与治疗前相比,治疗后1 d、7 d、14 d急性加重期COPD患者血清 CRP[（12．32±6．59）mg／L、（9．05±3．31）mg／L、（7．31±2．31）mg／L、（4．45±1．54）mg／L]与PCT[（0．34±0．05）μg／L、（0．26±0．04）μg／L、（0．18±0．04）μg／L、（0．10±0．03）μg／L]水平呈下降趋势,不同时间点差异有统计学意义（均P＜0．05）;与稳定期患者相比,治疗前、治疗后1 d、7 d血清CRP与PCT水平均明显高,差异有统计学意义（均P＜0．05）;但治疗后14 d血清CRP与PCT水平差异无统计学意义（P＞0．05）。结论急性加重期COPD患者早期血清CRP及PCT水平明显增高,治疗后其水平明显下降,联合观察血清CRP及PCT水平可以更好地反映COPD急性加重期的炎性反应。     

COPD患者加重期与稳定期YKL40水平变化研究

目的探讨加重期和稳定期COPD患者在YKL40水平方面的差异。方法采用ELISA法检测2013年1月~2014年12月在我所住院的COPD加重期(AECOPD)的患者60例,COPD稳定期的患者59例及我所体检中心正常患者(对照组)50例血清中YKL-40的表达水平。分析COPD患者血清中YKL-40水平与COPD发病危险因素的相关性。结果实验结果显示,在对照组、COPD组和AECOPD组血清中YKL-40水平比较中,差异有统计学意义(P0.01),AECOPD组和COPD组血清中YKL-40水平比较显示,差异有统计学意义(P0.01);AECOPD组血清中YKL-40水平比COPD组血清中的YKL-40水平高,差异有统计学意义(P0.01)。AECOPD组患者血清YKL-40含量与FEV1/FVC呈负相关(r=-0.605,P0.001),与FEV1%呈负相关(相关系数r=-0.525,P=0.003)。COPD组患者血清YKL-40含量与FEV1/FVC呈负相关(r=-0.601,P0.001),与FEV1%呈负相关(r=-0.466,P=0.009)。结论 COPD患者患病不同时期体内YKL40水平不一,对于临床后续治疗和相关药物的使用具有指导意义,值得深入研究。     

血清降钙素原联合C反应蛋白检测对儿童川崎病并发冠状动脉损害的预测意义探讨

目的探讨分析血清降钙素原(PCT)联合C反应蛋白(CRP)检测预测儿童川崎病(KD)并发冠状动脉损害(CAL)的临床意义。方法80例KD患儿为研究对象,以是否并发CAL分为基础组(未并发CAL)及研究组(并发CAL),各40例。两组患儿均进行血清PCT、CRP检测。比较两组患儿的血清PCT、CRP水平,血清PCT、CRP阳性情况,分析血清PCT、CRP联合预测KD并发CAL的价值。结果研究组患儿的血清PCT(1.64±0.69)μg/L、CRP(23.66±6.21)mg/L均高于基础组的(1.01±0.77)μg/L、(14.21±6.74)mg/L,差异均具有统计学意义(P<0.05)。两组患儿的血清PCT阳性率比较,差异无统计学意义(P>0.05);研究组患儿的CRP阳性率为80.00%,高于基础组的57.50%,差异具有统计学意义(P<0.05)。血清PCT、CRP升高均为KD并发CAL的独立危险因素[OR=1.040,95%CI=(1.010,1.074),P=0.002<0.05]、[OR=1.074,95%CI=(1.024,1.211),P=0.000<0.05]。结论血清PCT联合CRP检测能够取得较为准确、有效的KD并发CAL参考数据,进而为早期预测KD并发CAL提供帮助,改善KD患儿预后,临床意义较大。     

CRRT辅助治疗脓毒性休克患者的临床疗效及其对血清PCT与CRP的影响

目的 评价分析连续性肾脏替代疗法(CRRT)辅助治疗脓毒性休克患者的临床疗效及其对血清降钙素原(PCT)与C反应蛋白(CRP)的影响。方法 68例脓毒性休克患者,采取随机数字表法分为对照组和观察组,每组34例。对照组患者采取常规方法治疗,观察组患者在对照组基础上采取CRRT辅助治疗。比较两组患者的临床疗效及治疗前后血清PCT和CRP水平。结果 观察组患者的治疗总有效率为94.12%,显著高于对照组的67.65%,差异具有统计学意义(P<0.05)。观察组患者治疗前血清PCT水平为(84.56±3.98)μg/L、CRP水平为(181.36±32.87)mg/L,治疗后分别为(37.36±3.07)μg/L、(12.31±3.79)mg/L;对照组患者治疗前血清PCT水平为(84.54±3.96)μg/L、CRP水平为(179.25±35.91)mg/L,治疗后分别为(67.34±3.25)μg/L、(26.34±5.12)mg/L;治疗前,两组患者的血清PCT、CRP水平比较,差异无统计学意义(P>0.05);治疗后,两组患者的血清PCT、CRP水平均低于治疗前,且观察组显著低于对照组,差异均具有统计学意义(P<0.05)。结论 对于脓毒性休克患者,给予CRRT辅助治疗具有显著的疗效,且有助于患者血清PCT与CRP水平的改善,值得采纳及应用。     

检测PCT和CRP在哮喘合并呼吸道感染诊断和治疗的意义

目的探讨血清降钙素原(PCT)和C反应蛋白(CRP)在哮喘合并呼吸道感染中的临床意义。方法哮喘合并呼吸道感染患者120例均分为哮喘合并细菌感染(A)组和哮喘合并病毒感染(B)组,单纯哮喘患者60例作为对照(C组),测定并比较血清PCT和CRP水平。结果 A组血清PCT和CRP浓度为(26.5±8.7)μg/L和(50.1±18.6)mg/L,均高于B组的(0.6±0.2)μg/L和(5.8±0.6)mg/L和C组的(0.3±0.1)μg/L和(4.1±0.3)mg/L(P<0.01);A组血清PCT和CRP阳性检出率高于B、C组(P<0.01),B组高于C组(P<0.05)。结论哮喘合并细菌感染患者血清PCT和CRP水平明显升高,检测PCT和CRP可为临床使用抗菌药物提供参考依据。     

淋巴细胞比值、 C反应蛋白及血清降钙素原在系统性红斑狼疮合并细菌感染病人早期诊断中的应用

目的 探讨淋巴细胞比值(NLCR)、C反应蛋白(CRP)及血清降钙素原(PCT)在系统性红斑狼疮(SLE)合并细菌感染病人早期诊断中的应用.方法 纳入2017年3月至2019年8月安庆市立医院收治的92例SLE病人的临床资料,将病例依据是否合并细菌感染分为SLE合并细菌感染组49例和SLE未合并细菌感染组43例.记录所有病人的性别、年龄、主诉、系统受累程度等一般资料,检测中性粒细胞、淋巴细胞数量、PCT及CRP水平.结果 SLE合并细菌感染组病人的肾炎、雷诺综合征发生率、NLCR(6.12±2.84)、PCT(1.92±1.56)μg/L及CRP(31.69±18.25)mg/L水平均高于SLE未合并细菌感染组病人[NLCR(4.42±2.28)、PCT(0.25±0.33)μg/L及CRP(17.45±11.58)mg/L],均差异有统计学意义(P<0.05).经Pearson相关性检验,结果 显示,NL-CR、CRP与PCT均呈低度正相关(r=0.219,P=0.035;r=0.231,P=0.027).NLCR、PCT及CRP诊断SLE合并细菌感染的ROC曲线结果 显示,NLCR、PCT、CRP及三者联合的AUC分别为:0.682、0.869、0.740、0.934;同时,三指标联合显著高于各单一指标.结论 NLCR、CRP与PCT均可用于SLE合并细菌感染病人早期诊断,且三指标联合检测可大大提高诊断价值.     

联合检测血清PCT、CRP在细菌感染引起AECOPD患者的诊断价值

目的探讨检测血清降钙素原（procalcitonin,PCT）、C-反应蛋白（C—reactiveprotein,CRP）水平对指导细菌感染引起慢性阻塞性肺疾病急性加重期（acuteexacerbationofchronicobstructivepulmonarydisease,AECOPD）患者临床诊治中的意义。方法对AECOPD患者58例（A组）及COPD稳定期患者31例（B组）,采用免疫发光法测定其血清PCT及CRP水平,并进行诱导痰细菌定量培养;以痰中下呼吸道潜在病原菌（PPM）浓度107CFU／mL作为诊断AECOPD细菌感染的标准,将AECOPD患者分为有细菌感染组（Al组35例）、无细菌感染组（A2组23例）。结果AECOPD有细菌感染组血清PCT（0．22±0．03）ng／mL,CRP（59．79±10．23）mg／mL水平高于无细菌感染组PCT（0．11±0．02）ng／mL,CRP（18．34±3．15）mg／mL及稳定期组PCT（0．08±0．01）ng／mL,CRP（10．06±1．63）mg／mL,差异有统计学意义（P〈0．05）。COPD急性加重期无细菌感染组PCT（0．11土0．02）ng／mL水平高于稳定期组PCT（0．08±0．01）ng／mL,差异无统计学意义（P〉0．05）,急性加重期无细菌感染组CRP（18．34±3．15）mg／mL水平高于CRP（10．06±1．63）mg／mL,差异有统计学意义（P〈0．05）。结论COPD患者PCT水平升高可能与细菌感染有关、而CRP水平升高可能提示急性加重,联合检测血清PCT及CRP可以帮助了解AECOPD的细菌感染及指导抗生素应用。     

血清降钙素原在新生儿感染早期诊断中的应用价值分析

目的 分析血清降钙素原(PCT)在新生儿感染早期诊断中的应用价值。方法 选取80例感染患儿作为观察组,另选同期80例非感染患儿作为对照组。对比两组患儿血清PCT、C反应蛋白(CRP)检测水平,计算PCT与CRP的曲线下面积、最佳临界值、灵敏度、特异度、阳性预测值及阴性预测值、约登指数,并进行比较。结果 观察组患儿PCT、CRP检测水平分别为(5.71±3.28)μg/L、(17.54±11.26)mg/L,均显著高于对照组的(0.41±0.28)μg/L、(3.42±2.85)mg/L,差异均有统计学意义(P<0.05)。PCT的ROC曲线下面积为0.876,最佳临界值为0.52μg/L, CRP的ROC曲线下面积为0.693,低于PCT,最佳临界值为7.98μg/L,高于PCT。PCT的灵敏度为91.28%,特异度为75.61%,阳性预测值为75.34%,阴性预测值为88.14%,约登指数为0.64;CRP的灵敏度为64.03%,特异度为84.85%,阳性预测值为80.12%,阴性预测值为66.35%,约登指数为0.47;比较差异有统计学意义(P<0.05)。结论 血清PCT在新生儿感染早期诊断中具有较高临床应用价值,值得采纳应用。     

降钙素原、C反应蛋白和D-二聚体水平检测在危重症患儿感染中的临床意义

目的探讨血清降钙素原（PCT）、C反应蛋白（CRP）和血浆D-二聚体（D-D）水平的检测在危重症患儿感染中的临床意义。方法采用免疫荧光法和免疫比浊法检测198例危重患儿血清PCT、CRP和血浆D-D。结果临床感染者（100例）患儿血清PCT、CRP和血浆D-D水平分别为（24.5±10.2）μg/L,（45.3±10.5）mg/L,（1.85±0.55）mg/L,均显著高于临床非感染者（98例）的（0.5±0.15）μg/L,（6.1±0.6）mg/L,（0.5±0.25）mg/L,差异有统计学意义（P〈0.05）。血清PCT、CRP和血浆D-D水平均与患儿的病情严重程度呈正相关（P〈0.05）。临床感染组患儿PCT、CRP、D-D阳性率分别为93（93%）、80（80%）、89（89%）,均高于临床非感染组35（35.7%）,25（25.5%）,40（40.5%）,差异有统计学意义（P〈0.05）。结论血清PCT、CRP和血浆D-D水平检测可作为早期诊断危重症患儿感染病情严重程度评估及疗效判断的重要敏感性指标。血浆D-D水平检测对早期判断危重患儿感染时凝血功能异常程度具有重要临床指导意义。     

老年慢性阻塞性肺炎急性加重患者外周血白细胞、中性粒细胞、C反应蛋白及降钙素原的测定及临床意义

目的：探讨外周血白细胞（ WBC）、中性粒细胞（ NEUT）、C反应蛋白（ CRP）和血清降钙素原（ PCT）在老年慢性阻塞性肺炎急性加重期（ AECOPD）水平发生变化的临床意义。方法测定320例老年AECOPD患者、40例对照组研究对象的WBC、NEUT、CRP及PCT,并测定老年AECOPD组抗感染治疗后上述指标。结果老年AECOPD组血WBC（10．98±8．88）×109· L－1、NEUT（9．23±9．19）×109· L－1、CRP（73．42±55．42） mg· L－1及PCT（2．76±9．41）μg· L－1水平高于对照组血WBC （6．54±1．88）×109· L－1、NEUT（3．78±0．97）×109· L－1、CRP （9．75±13．42）mg· L－1及PCT （0．14±0．14）μg· L－1,差异有统计学意义。抗感染治疗后WBC、NEUT、CRP和PCT分别降至（8．90±8．64）×109· L－1、（7．55±9．13）×109· L－1 L－1、（41．70±4．07） mg· L－1和（1．04±2．34）μg· L－1。结论 WBC、NEUT、CRP和PCT出现异常升高,是细菌感染的警示指标,对感染的早期识别有重要的参考价值。     

联合检测血清Hcy和Lp (a)与UA对冠心病患者预后评估的价值探讨

目的 探讨血清同型半胱氨酸(Hcy)、脂蛋白a[Lp(a)]和尿酸(UA)联合检测对冠状动脉粥样硬化性心脏病(CHD)的病情及预后的评估价值.方法 选取CHD患者96例(疾病组)和体检健康者50例(正常组),采集清晨空腹静脉血,检测血清Hcy、Lp(a)和UA.观察组(疾病组)随访1年,91例病情缓解或无进展,5例发展为急性心肌梗死(AMI).采用受试者工作特征曲线分析血清Hcy、Lp(a)和UA单独及联合检测对CHD患者预后的诊断价值.结果 疾病组血清Hcy、Lp(a)和UA水平均高于正常组(均P＜ 0.05);随访发展为AMI患者入院时血清Hcy、Lp(a)和UA水平均高于病情缓解或无进展者(均P＜ 0.01).Hcy、Lp(a)和UA联合检测对CHD患者预后评估的AUC为0.855、敏感性为89.4％、特异性为80.2％、阳性预测值86.4％,均高于3项单独检测.结论 冠状动脉粥样硬化性心脏病患者血清Hcy、Lp (a)和UA水平均升高,其水平变化可反映冠状动脉粥样硬化性心脏病病情,三者联合检测可提高对患者预后的判断价值.     

Comparison of the inhibition potential of parthenolide and micheliolide on various UDP-glucuronosyltransferase isoforms

Abstract

1. Parthenolide (PTL) and micheliolide (MCL) are sesquiterpene lactones with similar structures, and both of them have been reported to exhibit multiple biochemical and pharmacological activities. This study aims to investigate the inhibition of these two compounds on the activity of UDP-glucuronosyltransferases (UGTs).

2. In vitro incubation mixture for recombinant UGTs-catalyzed glucuronidation metabolism of 4-methylumbelliferone (4-MU) was utilized to investigate the inhibition potential. Inhibition kinetics (including inhibition type and parameters) were determined, and in silico docking was employed to elucidate the inhibition difference between PTL and MCL on UGT1A1.

3. MCL showed no inhibition toward all the UGT isoforms, and PTL showed strong inhibition toward UGT1A1. The half-maximal inhibitory concentration (IC₅₀) of PTL on the activity of UGT1A1 was determined to be 64.4?μM. Inhibition kinetics determination showed that PTL exerted noncompetitive inhibition toward UGT1A1, and the inhibition kinetic constant (K_i) was determined to be 12.1?μM. In silico docking method has been employed to show that hydrogen bonds between PTL and the activity cavity of UGT1A1 contributed to the stronger inhibition of PTL on the activity of UGT1A1 than MCL. In conclusion, PTL can more easily induce drug–drug interaction (DDI) with clinical drugs mainly undergoing UGT1A1-catalyzed glucuronidation.

     

The effect of halothane on the stability of Ca2+ transport activity of isolated fragmented sarcoplasmic reticulum

The effects of the inhalation anaesthetic agent, halothane (CF₃CHBrCl), on the stability of the calcium transport system of isolated rabbit white skeletal muscle sarcoplasmic reticulum have been studied. Calcium transport activity was unaffected when suspensions of sarcoplasmic reticulum vesicles were preincubated at 37° and pH 6.8 at concentrations of halothane below 5 mM, but was progressively inactivated at higher concentrations. (Ca²⁺,Mg²⁺)-ATPase activity was enhanced during inactivation of calcium transport. At pH 6.3 and 5.8, halothane increased the first order rate constants of inactivation and effects were noted in the anaesthetic range of concentration (1–2 mM). The inulin inaccessible space of membrane vesicles did not change appreciably during the period of treatment with halothane, excluding increased permeability as an explanation of the inhibition of calcium accumulation. Inactivation was irreversible and highly temperature dependent, with an activation energy of 52.7 kcal/mol. Calcium ions had a protective effect against inactivation (K_{0.5 (Ca2+) = 1.5 × 10^?6M}), as did ATP ($\text{K}{}_{\mathrm{<mtext>0.5\; (</mtext><mtext>Atp</mtext><mtext>)</mtext>}}\text{? 10}{}^{\mathrm{?6}}\text{M}$). It is concluded that mild acid conditions and halothane act synergistically during inactivation of the calcium transport system of sarcoplasmic reticulum membranes. These studies suggest that halothane interacts with the (Ca²⁺, Mg²⁺)-ATPase protein at the ATP-specific binding site or that it disrupts protein-lipid associations in the membrane. In either case the destabilizing effect of halothane may be modified by the conformational state of the protein.     

运动对糖尿病肾病早期相关指标的影响

目的研究运动对糖尿病肾病(DN)早期诊断指标如尿微量白蛋白(Alb)、尿转铁蛋白(TRF)、尿铜蓝蛋白(CP)、尿视黄醇结合蛋白(RBP)的影响。方法对45名2型糖尿病患者做次极量运动试验,运动前后分别测尿Alb、尿TRF、尿CP、尿RBP及尿肌酐(Cr),并以26名非糖尿病人做对照。结果糖尿病患者运动后尿Alb/Cr较运动前增加(P>0.01),尿TRF/Cr,CP/Cr,RBP/Cr运动前后无明显改变;非糖尿病人运动后尿Alb/Cr也较运动前增加(P>0.05),尿TRF/Cr,CP/Cr,RBP/Cr运动前后也无明显改变。而在相关分析中发现,尿RBP/Cr与Alb/Cr无显著相关性。尿TRF/Cr,CP/Cr与Alb/Cr有显著相关性。结论尿Alb/Cr受运动影响而尿TRF/Cr、CP/Cr不受运动影响,可作为DN早期诊断指标之一。     

Theoretical π-π* absorption and circular dichroic spectra of cyclic dipeptides

The dipole interaction model, treated by the partially dispersive normal mode method, is used to calculate circular dichroic spectra of cyclo(Gly-Gly), cyclo (Ala-Gly), cyclo(Ala-Ala), cyclo(Pro-Gly), cyclo(Pro-Ala), cyclo(Pro-Val), cyclo (Pro-D-Val), and cyclo(Pro-Pro) in the amide π-π* absorption band near 190 nm. Assuming a standard backbone geometry, spectra which are in fair to good agreement wth experiment are obtained for these molecules. The spectra are predicted to be sensitive to conformations of Pro and Val side chains. The effects of dipeptide ring folding on calculated CD spectra are mostly consistent with those found by other workers, except that it is found that a planar ring conformation of cyclo (Ala-Ala) and cyclo (Ala-Gly) gives predicted spectra comparable to experiment. The same model gives theoretical absorption spectra consistent with available experimental data.     

AFP、AFU、DSA-GGT联合检测对原发性肝癌的诊断价值

目的探讨甲胎蛋白(AFP)、α-L-岩藻糖苷酶(AFU)和欧蔓陀罗凝集素(DSA)强结合的γ-谷氨酰转移酶(DSA-GGT)联合检测对原发性肝癌(PHC)的诊断价值。方法对58例PHC和167例良性肝病患者,以及36例其它肿瘤患者,进行AFP、AFU和DSA-GGT指标联合检测,分析3项指标对PHC的诊断价值。结果PHC组的AFP、AFU、DSA-GGT的阳性率均明显高于良性肝病和其它肿瘤组(P<0.01);AFP、AFU和DSA-GGT对PHC的诊断敏感性分别为68.97%、67.24%和65.52%,特异性分别为90.64%,89.66%和91.13%;PHC患者血清AFP、AFU、DSA-GGT之间无相关性,联合检测对PHC有互补诊断价值,阳性率达96.55%。结论AFP、AFU和DSA-GGT等3个指标联合应用,可提高PHC的诊断灵敏度,提高诊断水平。     

GC法检测浙八味药材中有机氯农药的残留

目的 测定与分析白术、白芍、玄参等8种药材中9种有机氯农药残留量.方法 以混合溶剂超声提取样品、浓硫酸磺化净化,采用毛细管气相色谱,用HP-5弹性石英毛细管柱程序升温分离,微电子捕获检测器检测,峰面积外标法定量.结果 延胡索、麦冬和杭白菊均检出滴滴涕,含量分别为0.439、45.017、5.434 ng·g<'-1>;杭白菊检出五氯硝基苯,含量为1.030 ng·g<'-1>;其余药材均未检出有机氯农药残留.结论 浙八味药材大部分未检出有机氯农药残留或含量较低,均在规定的安全范围内.     

吸烟对血液过氧化与抗氧化平衡的影响

比较吸烟与不吸烟人静脉血血浆及红细胞脂质过氧化物-丙二醛（MDA）含量，血浆及红细胞超氧化物歧化酶（SOD）活力、全血谷胱甘肽过氧化物酶（GSH－Px）活力及血浆总抗氧化能力（AOA）．结果表明：吸烟组血浆及红细胞MDA的含量高于不吸烟组（P＜0．01），红细胞SOD含量高于不吸烟组（P＜0．01），血浆SOD、全血GSH－Px活力．血浆AOA均低0于不吸烟组（P＜0．01，P＜0．05，P＜0.05）．提示长期吸烟可使血中氧化与抗氧化失去平衡。     

HPLC法测定丝裂霉素C聚氰基丙烯酸正丁酯纳米粒中丝裂霉素C的含量

目的:建立高效液相色谱法测定丝裂霉素 C 聚氰基丙烯酸正丁酯纳米粒(MMC-PBCA-NP)中药物含量。方法:采用C_(18)柱(4.6 mm×150 mm,5 μm),以混合磷酸盐缓冲液-乙腈(85:15)为流动相,流速为1 mL·min~(-1),紫外检测器,检测波长为365 nm。结果:丝裂霉素 C(MMC)浓度在5～250 μg·mL~(-1)范围内与峰面积呈良好的线性关系,r=0.9998;平均回收率(n=6)为98.15%。结论:本法专属性强,操作简便,结果准确。适用于 MMC-PBCA-NP 的质量控制。     

Anti-ischemic effects of nimesulide, a cyclooxygenase-2 inhibitor on the ischemic model of rabbit induced by isoproterenol

The objective was to devise an animal model of myocardial infarction (MI) against which cardioprotective drugs might be tested. We describe the effects of nimesulide, a COX experience with development and validation of such a model. The rabbit was chosen in preference to rodents because its heart and cardiac circulation more closely resemble those of human. Thus, the cardiovascular system of anaesthetized male rabbits, 1 to 1.5 kg (n=11), was stressed by a single bolus intravenous injection of isoprenaline (ISP), 65 mg/kg. The effects of the injection were followed for sixteen days and were evaluated in four ways: 1) measurements of creatinine kinase isozyme and troponin-I (TPI) in serum 2) Electrocardiographic (ECG) changes (ST elevation and Q wave development) 3) Cardiac histopathology observed in tissue sections of the isolated of the heart. The histopathological analysis showed that rabbit heart on 2nd day after ISP injection showed changes of coagulation necrosis. Day 4 total coagulation with the loss of nuclear and striation associated with heavy interstitial infiltrate of neutrophils was found. Day 8 after infarction showed collagen deposition with capillary channels in between the remaining islands of myocytes in the infarcted area. On the 16th day scarring was complete. Coronary perfusion rates (CPR) and heart rate (HR) of the infarcted and nimesulide (a COX-2 inhibitor) treated rabbits displayed significant improvement (n=11) on each corresponding day after infarction as compared to the infarcted and saline treated rabbits (P<0.05). All four indices revealed similarities with effects commonly associated with MI in humans.