Inverse Association between Serum Selenium Level and Severity of Liver Fibrosis: A Cross-Sectional Study

Selenium has been well recognized for its important role in human health. Prior studies showed that low serum selenium was associated with various diseases, including cardiovascular disease, cancer, infertility, and cognitive decline. Recent studies demonstrated an association between selenium deficiency and liver cirrhosis. In our study, we aimed to explore the association between serum selenium levels and severity of liver fibrosis. In total, 5641 participants at an age of 12 and above, from the 2017–2018 United States National Health and Nutrition Examination Survey, were enrolled. The severity of liver fibrosis was determined by liver ultrasound transient elastography. There was a significant linear decrease in liver stiffness measurement (LSM) values in male groups with increased serum selenium levels. The beta coefficient (β) = −1.045 in male groups. A significantly negative association was also observed in the group of age ≥ 60. In addition, those in the highest quartile of serum selenium had lower LSM values (β = −0.416). This is the first study using LSM to demonstrate the correlation between selenium deficiency and severity of liver cirrhosis. Our findings suggest that a high plasma selenium concentration is negatively correlated with the severity of liver cirrhosis and there are gender and age differences.     

二硫化碳接触对粘胶工人血清铜、锌和硒含量的影响

目的 探讨CS₂接触与血清微量元素铜、锌、硒之间的关系,为CS₂接触致心血管系统疾病的毒作用机理提供依据.方法 选择某化纤厂纺丝车间年龄在45岁以下,接触CS₂5年以上76名CS₂作业工作为接触组;在同一工厂棉浆车间选择曾经接触过CS₂但现在已停止CS₂接触5年以上的33名工人作为干预组;从未接触过CS₂的23名工人作为对照组进行流行病学横断面调查.结果 接触组SCu、SZn和SSe含量均低于对照组(P=0.0001);多元逐步回归分析进一步显示,CS₂对SCu、SZn和SSe含量均有负贡献(偏回归系数β分别为-10.4173,-8.973和-15.5098).结论 提示CS₂接触能降低血清铜、锌、硒含量.     

Genetic Variation Interacts with Selenium Exposure Regarding Breast Cancer Risk: Assessing Dietary Intake,Serum Levels and Genetically Elevated Selenium Levels

Selenium has been suggested to be protective regarding breast cancer risk but no overall effect has been established. Genetics may modify the effect. This study compares the effect of selenium exposure on breast cancer risk between women with different alleles in single-nucleotide polymorphisms (SNPs). The Malmö Cancer and Diet Study, a cohort including 17,035 women and >25 years of follow-up on breast cancer diagnosis, was used. Five promising SNPs regarding interaction with selenium exposure were selected from the literature: rs1050450, rs4880, rs3877899, rs7579, and rs71304. Selenium exposure was assessed in three ways: genetically elevated (n = 16,429), dietary intake (n = 15,891) and serum levels (n = 2037) at baseline. Cox regression and logistic regression analyses evaluated breast cancer risk from selenium exposure, stratified for the SNPs and adjusted for risk factors. A total of 1946 women were diagnosed with breast cancer. Women with T/T alleles in rs1050450 had lower breast cancer risk compared with C/C, HR 0.81 (0.68–0.96). Interaction by rs1050450 limited a protective effect of higher selenium intake to T/T carriers, HR 0.68 (0.43–1.08) for intermediate intake and HR 0.63 (0.40–1.00) for high intake. No interactions or risk differences were seen for other SNPs or for serum selenium or genetically elevated selenium. The results indicate that genetic variation in rs1050450 might affect breast cancer risk and that selenium exposure could be a possible modifiable risk factor for breast cancer among women with that variation.     

护理干预对子宫内膜癌患者癌因性疲乏的影响

目的 探讨护理干预对子宫内膜癌术后病人癌因性疲乏的影响.方法 将36例子宫内膜癌术后患者随机分为常规组和干预组,常规组给予常规的一般护理及健康教育指导,干预组给予系统的护理干预,比较两组患者疲乏程度和生活质量.结果 干预组较常规组疲乏症状明显改善,总体生活质量显著高于常规组.结论 通过护理干预可以明显改善子宫内膜癌患者的癌因性疲乏.     

Selenium Fortification of an Italian Rice Cultivar via Foliar Fertilization with Sodium Selenate and Its Effects on Human Serum Selenium Levels and on Erythrocyte Glutathione Peroxidase Activity

Selenium food fortification could be a cost-effective strategy to counteract the inadequacy of selenium intake among the Italian population. In this study, the effect of foliar fertilization with sodium selenate of an Italian rice cultivar and the increase of serum selenium and of erythrocyte glutathione peroxidase (GPx) activity after intake of fortified rice, have been evaluated. The effect of foliar fertilization with sodium selenate (50 g Se/ha) vs. water was studied. Moreover, in a randomized, double-blind study, 10 healthy women supplemented their usual diet with a daily dose of 80 g of Se-enriched-rice and 10 matched-women with 80 g of regular rice. Before, after 5 and 20 days of supplementation, serum Se and GPx-activity were evaluated. The mean selenium content in Se-enriched-rice was 1.64 ± 0.28 μg/g, while in regular rice it was 0.36 ± 0.15 μg/g (p < 0.001). A significant increase of serum Se and GPx-activity was observed only in the intervention group and only after 20 days. The results show that selenium fortification of rice can be achieved with foliar fertilization with sodium selenate and that the 20 days intake of this Se-enriched-rice increases the serum selenium levels and GPx-activity.     

经阴道超声在子宫内膜癌及子宫内膜增生症鉴别中的应用

目的 本文旨在研究经阴道超声在鉴别子宫内膜癌及子宫内膜增生症中的应用效果情况,分析其在临床后续治疗中的应用价值.方法 回顾性选取2018年9月-2019年7月我院住院治疗的已知为子宫内膜病变的患者共94例,根据其病理结果将其分为A组(子宫内膜癌45例)及B组(子宫内膜增生症49例).两组患者均进行经阴道彩色多普勒超声检...     

Daily Intake and Serum Levels of Copper,Selenium and Zinc According to Glucose Metabolism: Cross-Sectional and Comparative Study

Trace elements play an important role in metabolism. We compared the daily intake and serum concentrations of copper (Cu), selenium (Se), and zinc (Zn) across a spectrum of glucose tolerance status in a representative U.S. population. Daily intake and serum concentrations of Cu, Zn and Se in 5087 adults from the 2011–2016 National Health and Nutrition Examination Survey (NHANES) were examined and compared to normal (NGT) and abnormal (AGT) glucose tolerance and the presence of diabetes mellitus (DM). Other than Zn deficiency (21.15%), the prevalence of Zn, Se, and Cu excess and Se and Cu deficiency were low (<4.00%). As compared to the NGT group, Cu and Se supplementation was higher in the AGT and DM groups (p < 0.0001 for all). Serum Se and Zn, but not Cu, concentrations were highly correlated with daily intake (p < 0.0001 for both). As compared to the NGT group, serum Cu concentration was highest in the AGT group (p = 0.03), serum Se concentration was highest in the DM group (p < 0.0001), and serum Zn concentration was highest in the AGT group (p < 0.0001). Serum Se and Zn concentration was correlated with daily Se and Zn intake. Even within the reference range for serum Cu, Se, and Zn concentrations, a higher serum concentration of Cu, Se, and Zn was associated with abnormal glucose metabolism. Although the casual relationship remains to be elucidated, these data suggest caution in Cu, Se and Zn supplementation in non-deficient individuals.     

深圳市孕妇血清微量元素分析

目的 :探讨深圳市孕妇在不同孕期血清微量元素变化规律。方法 :按孕周将 6 4 8名孕妇分为早孕 (小于16周 )、中孕 (16～ 2 8周 )、晚孕 (大于 2 8周 ) 3个实验组 ,132名正常体检妇女作为对照组 ,采用原子吸收光谱法测定孕妇及对照组妇女血清微量元素含量 ,比较实验组及对照组血清微量元素值。结果 :孕妇在早孕期血清铜含量及铜 /锌比值显著升高 ,并随孕周增加进一步升高 ;血清锌、钙、镁含量则显著下降 ,并随孕周增加进一步降低 ;血清铁含量在早孕期与对照组比较无显著差异 ,而从中孕期开始显著下降。随着孕周的增加 ,缺乏锌、铁、钙、镁的孕妇比例也升高。结论 :深圳市孕妇早孕期起即应注意锌、钙、镁元素的补充 ,中孕期起应注意加强铁营养的补充。     

硒对胃癌化疗敏感性影响的研究进展

胃癌因在我国各类恶性肿瘤中的高发病率及高死亡率而倍受关注.由于胃癌单纯的手术治愈率不高,且易出现复发或转移,因此,化疗是其主要的治疗手段之一.但化疗的不良反应太大,致使很多患者不能完成治疗.硒是人体所必需的营养元素,同时也是一种广泛的抗癌因子,目前人们对硒的研究,主要侧重于作为癌症化疗辅助剂的作用,硒能明显降低化疗药物的不良反应,并且在协同化疗药物抗肿瘤方向发挥着重要的作用.     

饲喂不同食物的小鼠血清微量元素水平变化的实验研究

目的 :研究饲喂不同食物的小鼠血清中微量元素含量水平的变化。方法 :将小鼠随机分成对照组、实验A组、实验B组 ,分别饲喂正常饲料、加喂鸡蛋、加喂鸡蛋及葵花子仁 ,应用原子吸收光谱法测定 3组小鼠血清中锌、铜、铁、钙、镁的含量。结果 :两个实验组雌、雄小鼠血清中的铁含量水平均对应高于对照组的雌、雄小鼠 ,锌均对应低于对照组 ,锌、铁之间呈负相关。实验B组雌性小鼠的铜、铁均高于实验A组和对照组的雌、雄性小鼠及同组的雄性小鼠。结论 :经常食用鸡蛋、葵花子仁等天然食品 ,可能提高人们特别是妇女体内一些必需微量元素的含量水平 ,有益于人体健康     

卵巢子宫内膜异位囊肿血清糖链抗原-125的测定及其临床意义

目的 探讨血清糖链抗原-125 (CA125)与卵巢子宫内膜异位囊肿(ECO)的关系.方法 选择2009年7月至2010年12月于本院妇科诊治的62例ECO患者为研究对象,纳入研究组;选择同期在本院住院手术治疗的卵巢良性囊肿[不合并子宫内膜异位症(EMs)]患者62例,纳入对照组,均经术后组织病理学检查结果证实(本研究遵循的程序符合本院人体试验委员会所制定的伦理学标准,得到该委员会批准,分组征得受试对象本人的知情同意,并与之签署临床研究知情同意书).两组患者年龄、体重指数(BMI)等比较,差异无统计学意义(P＞0.0 5).采用微粒子酶免疫分析法分别测定两组患者的血清CA125水平,并进行相关统计学分析.结果 研究组血清CA125水平显著高于对照组[(95.36±56.31)U/mL vs.(18.64±7.45) U/mL],两组比较,差异有统计学意义(P＜0.01).研究组血清CA125水平与EMs分期(病情严重程度)呈显著正相关关系(r=0.8832,P＜0.01),并且EMsⅡ～Ⅳ期患者的血清CA125水平较EMs Ⅰ期显著升高,差异有统计学意义(P＜0.01);EMsⅡ期与EMsⅢ期患者血清CA125水平比较,差异亦有统计学意义(P＜0.01).研究组血清CA125水平与痛经评分(DSS)呈正相关关系(r=0.5650,P＜0.01),并且DSS为1～3分患者与0分患者的血清CA125水平比较,差异均有统计学意义(P＜0.01).结论 血清CA125与ECO的发生发展及预后有关.ECO患者的血清CA125水平随着EMs分期(EMs严重程度)增加而升高,与DSS均呈正相关关系.测定血清CA125有助于对ECO的诊断及对病情、预后的判断.     

Associations between Maternal Selenium Status and Cord Serum Vitamin D Levels: A Birth Cohort Study in Wuhan,China

Serum selenium (Se) has been reported to be associated with serum 25-hydroxyvitamin D [25(OH)D], but epidemiological findings are limited in pregnant women. We aimed to assess the associations between maternal urinary Se concentrations and cord serum 25(OH)D levels. We measured urinary concentrations of Se in the first, second, and third trimesters and cord serum 25(OH)D of 1695 mother-infant pairs from a prospective cohort study in Wuhan, China. The results showed that each doubling of urinary Se concentrations in the first, second, third trimester, and whole pregnancy (average SG-adjusted concentrations across three trimesters) were associated with 8.76% (95% confidence interval (CI): 4.30%, 13.41%), 15.44% (95% CI: 9.18%, 22.06%), 11.84% (95% CI: 6.09%, 17.89%), and 21.14% (95% CI: 8.69%, 35.02%) increases in 25(OH)D levels. Newborns whose mothers with low (<10 μg/L) or medium (10.92–14.34 μg/L) tertiles of urinary Se concentrations in whole pregnancy were more likely to be vitamin D deficient (<20 ng/mL) compared with those with the highest tertile (>14.34 μg/L). Our study provides evidence that maternal Se levels were positively associated with cord serum vitamin D status.     

The Associations between Metalloestrogens,GSTP1, and SLC11A2 Polymorphism and the Risk of Endometrial Cancer

Background: The incidence of endometrial cancer (EC) is still rising. Numerous risk factors including patient characteristics and molecular instability have been identified for EC. The presence of specific molecular markers allows specific diagnostic and prognostic approaches. Several single nucleotide polymorphisms (SNPs) have been identified to influence endometrial cancer risk. Metalloestrogens are metal ions which can mimic estrogen activity; however, their role in uterine pathologies remains unknown. This study aimed to investigate total blood trace elements levels and evaluate the distribution of selected genotypes in GSTP1 and SLC11A2 genes. Methods: This retrospective case-control analysis was carried out in peripheral blood samples of 110 women with endometrial cancer (EC; n = 21), uterine fibroma (n = 25), endometrial polyp (n = 48), and normal endometrium (n = 16). Analysis included measurement of metals and phosphor in serum, and of genetic polymorphisms in GST (rs1695) and SLC11A2 (rs224589) in DNA from white blood cells. Serum trace elements were measured using ICP-OES spectrometry. SNPs were identified using Taq Man real-time PCR genotyping assays. Results: The study confirmed higher age (OR 2.19, 95% CI 1.69–2.24), post-menopausal status (OR 1.89, 95% CI 1.36–1.94), and diabetes type 2 (OR 1.54; 95% CI 0.97–1.72) as independent risk factors for EC. We also found a high level of Cd (OR 1.49; 95% CI 1.31–1.63) and a low level of Co (OR 0.76; 95% CI 0.53–0.59) to be independent risk factors of EC. None of the tested polymorphisms of GSTP1 and SLC11A2 were associated with EC risk. However, high Cd (OR 1.21, 95% CI 1.15–1.29) and Ni (OR 1.07, 95% CI 1.05–1.18) serum levels were significantly associated with a SLC1A2 TG genotype, and high Cd levels with GSTP1 (OR 1.05, 95% CI 1.01–1.13).     

氢化物-原子荧光法测定植物及人发中的痕量硒研究

目的研究在捕集剂覆盖下低温焙烧富集后测定植物及人发中痕量硒的效果,探索测定植物和人发中痕量硒的新方法。方法采用氢化物?原子荧光法。选择最佳仪器工作条件,用捕集剂覆盖,低温焙烧,富集分离,对植物和人发样品中的痕量硒进行检测。结果克服了使用混合酸分解样品时,有机物质不能彻底分解给测定带来的不利影响,拟订了测定植物、人发等生物样品中痕量硒的方法步骤。结论该方法精密度和准确度均能满足测定要求。     

硒与谷胱甘肽过氧化物酶在妊娠高血压综合征中的变化

妊娠高血压症 (妊高征 )一直是孕产期威胁母婴健康和生命的主要并发症之一 ,发病率较高 ,至今病因未明。有文献报道 [1]微量元素缺乏影响孕妇血浆中氧化与抗氧化失衡导致妊高征的发生 [2 ]。本研究应用原子吸收法及细胞化学发光检测法测定妊高征孕妇血浆中硒水平及红细胞内谷胱甘肽过氧化物酶(GSH- Px)的浓度。旨在探讨三者间的关系及对预防妊高征发生的价值。1　对　象　与　方　法1 .1　对象　　 2 0 0 1年 4月至 2 0 0 2年 4月间我院住院分娩产妇 83例 ,分为两组。(1 )妊高征组 49例 ,其中轻、中、重度妊高征分别为 8、1 2、2 9例。无…     

血清铜锌硒与卵巢癌关系的研究

对 40例卵巢癌患者进行血清铜、锌、硒及铜 /锌比值测定 ,并以 48例健康成年女性为对照。测定结果进行单因素分析和非条件 Logistic多因素分析。单因素分析结果表明 ,卵巢癌患者血清锌、硒低于正常组 ,铜 /锌比值升高 ,而血清铜无显著差异 ;有负荷患者铜 /锌比值明显高于无负荷患者 ,而血清锌、硒无显著差异。回归多因素分析表明 ,硒、锌是卵巢癌发病的保护因素 ,揭示锌、硒在卵巢癌预防及辅助治疗上均有一定价值。     

Selenium,Copper, Zinc Concentrations and Cu/Zn,Cu/Se Molar Ratios in the Serum of Patients with Acute Ischemic Stroke in Northeastern Poland—A New Insight into Stroke Pathophysiology

Background: In recent years, trace elements (TEs) have gained considerable attention in the course of treatment and diagnosis of ischemic stroke. The purpose of the conducted research was to determine the trace mineral status (Se, Cu, Zn, Cu/Zn ratio, and Cu/Se ratio) in patients with acute ischemic stroke compared to the population of healthy people in the northeastern region of Poland. Materials and methods: 141 patients with acute ischemic stroke (AIS) and 69 healthy control subjects were examined. The serum concentrations of mineral components were assessed by the atomic absorption spectrometry method. Clinical parameters were updated based on medical records. Results: The serum Se and Zn concentrations were significantly decreased (p < 0.0001; p < 0.0001) in patients with AIS compared with healthy control subjects. However, no significant differences were revealed in terms of the serum Cu concentration (p = 0.283). As expected, we found that the serum Cu/Zn and Cu/Se molar ratios were significantly higher (p = 0.001; p < 0.0001) in patients with AIS compared with healthy control subjects. Conclusions: Disturbed metal homeostasis is a significant contributor to AIS pathogenesis. Furthermore, marked disruption of the serum Cu/Zn and Cu/Se molar ratios could serve as a valuable indicator of AIS patients’ nutritional status and oxidative stress levels.     

Selenium Metabolism in Cancer Cells: The Combined Application of XAS and XFM Techniques to the Problem of Selenium Speciation in Biological Systems

Determining the speciation of selenium in vivo is crucial to understanding the biological activity of this essential element, which is a popular dietary supplement due to its anti-cancer properties. Hyphenated techniques that combine separation and detection methods are traditionally and effectively used in selenium speciation analysis, but require extensive sample preparation that may affect speciation. Synchrotron-based X-ray absorption and fluorescence techniques offer an alternative approach to selenium speciation analysis that requires minimal sample preparation. We present a brief summary of some key HPLC-ICP-MS and ESI-MS/MS studies of the speciation of selenium in cells and rat tissues. We review the results of a top-down approach to selenium speciation in human lung cancer cells that aims to link the speciation and distribution of selenium to its biological activity using a combination of X-ray absorption spectroscopy (XAS) and X-ray fluorescence microscopy (XFM). The results of this approach highlight the distinct fates of selenomethionine, methylselenocysteine and selenite in terms of their speciation and distribution within cells: organic selenium metabolites were widely distributed throughout the cells, whereas inorganic selenium metabolites were compartmentalized and associated with copper. New data from the XFM mapping of electrophoretically-separated cell lysates show the distribution of selenium in the proteins of selenomethionine-treated cells. Future applications of this top-down approach are discussed.     

富硒米喂养的大鼠全血硒水平

目的 :探讨富硒米喂养的大鼠全血硒水平与免疫功能的关系 ,为富硒米用于防癌保健提供科学根据。方法 :30天喂养试验 :2、3-二氨基萘荧光分析法测全血硒水平 ;3 H- Td R掺入液闪法检测淋巴细胞转化。结果 :低、中、高剂量组及对照组的大鼠全血硒水平分别为 0 .575± 0 .1 54,0 .686± 0 .2 0 6,0 .894± 0 .1 80 ,0 .479± 0 .0 88μg/ml,且中、高剂量组显著性地高于对照组( P<0 .0 5,P<0 .0 1 ) ;低、中、高剂量组的淋巴细胞转化分别为 0 .762± 0 .461 ,0 .887± 0 .474,0 .791± 0 .539,0 .42 4± 0 .2 2 1 cpm万 /1 0 6细胞 ,且中剂量组显著性地高于对照组 ( P<0 .0 5) ;受试动物进食、活动等正常 ,动物体重、血常规亦正常 ,未见动物中毒和死亡情况。结论 :富硒米可提高受试动物的全血硒水平 ,血硒水平的提高有利于增强机体的免疫功能。富硒米不引起动物中毒和死亡 ,提示富硒米为无毒食品 ,可用于防癌保健。     

高原红细胞增多症患者血清微量元素含量测定

目的 :探讨高原红细胞增多症患者血清中微量元素含量的变化与发病的相关性。方法 :以Varian -6 4 0AAS原子吸收分光光度仪测定 11例高原红细胞增多症患者血清Zn、Cu、Fe、Mg等微量元素水平。结果 :红细胞增多症患者血清中 ,Zn、Cu、Fe含量显著低于正常对照组 ,Mg无明显变化。结论 :微量元素含量改变可能与红细胞增多症病情发展有着密切的联系