胃癌组织中miR-451与MIF的表达及与临床病理的关系

目的探讨胃癌组织中microRNA-451(miR-451)与巨噬细胞游走抑制因子(MIF)的表达及与临床病理的关系。方法收集2013年3月至2014年3月该院接受治疗的30例胃癌患者,取其肿瘤及肿瘤旁相关组织,采用RT-PCR法检测组织内的miR-451与MIF的表达水平,并分析其与胃癌临床病理的关系。结果肿瘤组织中的miR-451相对表达为(0.287±0.143),显著低于肿瘤旁组织的(0.520±0.257)(t=-4.021,P0.05);肿瘤组织中的MIF相对表达为(1.542±0.023),显著高于肿瘤旁组织的(0.631±0.027)(t=-23.183,P0.05);miR-451与MIF的相对表达呈现显著负相关性(t=-0.721,P0.05);肿瘤组织中miR-451及MIF与胃癌的组织类型(高、中、低分化)、TNM分期(Ⅰ+Ⅱ、Ⅲ+Ⅳ)、浸润深度(T1+T2、T3+T4)及淋巴结转移(有、无)相关(均P0.05)。结论胃癌组织中miR-451及MIF的表达与胃癌的组织类型、TNM分期、浸润深度及有无淋巴结转移相关,推测miR-451反向抑制MIF,进一步调控胃癌在人体内的发生及发展。     

miR-145在胃黏膜癌变中的表达及其意义

背景:胃癌的死亡率居恶性肿瘤第二位,寻求新的胃癌标记物和潜在的生物治疗靶点是目前胃癌的研究热点。目的:探讨miR-145在胃癌形成不同阶段中的表达及其临床意义。方法:选取2011年9月～2012年3月长宁区中心医院37例胃癌及其相应癌旁组织、8例慢性非萎缩性胃炎组织和7例异型增生组织,以RT-PCR法检测miR-145表达,并分析其与胃癌临床病理特征的关系。结果:与癌旁组织相比,胃癌组织中miR-145表达明显下调(P<0.05);与慢性非萎缩性胃炎组织相比,异型增生组织中miR-145表达明显下调(P<0.05)。胃癌组织miR-145表达与患者的性别、年龄、肿瘤浸润深度和淋巴结转移均无关(P>0.05)。结论:miR-145表达下调可能与胃癌的发生、发展相关。     

miRNA181a在肝细胞癌中的表达及其临床意义

目的探讨miR-181a基因表达与肝细胞癌发生发展以及肿瘤病理学特征之间的关系。方法提取30例肝细胞癌组织及癌旁组织和10例肝血管瘤患者的肝脏组织的总RNA,用Real time-PCR法检测miR-181a在上述组织中的表达情况。结果肝细胞癌组织中miR-181a表达与癌旁组织相比差异无统计学意义(P>0.05);与正常肝脏组织相比,miR-181a在肝细胞癌组织中的表达水平显著低于正常对照组织,差异有统计学意义(P<0.01);miR-181a表达下调与肝细胞癌分化程度、临床分期和合并肝硬化明显相关(P<0.05),与肝细胞癌的大小、血AFP浓度、HBV感染均无明显相关性(P>0.05)。结论 miR-181a表达下调参与肝细胞癌的发生发展过程,miR-181a异常表达与肝细胞癌的肿瘤分化程度、临床分期和合并肝硬化相关。     

miR-145在胃癌中的表达及其意义

目的:探讨微小RNA-145(miR-145)在胃癌中的表达及与临床病理特征和预后的关系.方法:收集62例胃癌患者肿瘤组织和配对癌旁组织标本,采用实时荧光定量-聚合酶链反应(q RT-PCR)方法检测miR-145的相对表达量,并分析其与临床病理资料和预后的关系.生存分析采用Kaplan-Meier法,并以Log-rank法比较组间差异,应用Cox模型进行多因素分析.结果:miR-145在胃癌组织中的表达水平显著低于配对癌旁组织(P0.01).miR-145的表达水平与临床分期、浸润深度及淋巴结转移呈明显相关(P0.05),与患者的年龄、性别、肿瘤大小、分化程度、民族均无明显相关(P0.05).miR-145高表达者生存时间明显高于低表达组(P0.05).多因素分析未显示单一的因素与患者的生存相关.结论:miR-145在胃癌组织中呈低表达,且与预后相关,可能参与了胃癌的发生、发展过程,可成为胃癌诊断及预后判断的指标.     

胃癌组织TRAF3、TRAF6阳性表达及意义

目的探讨胃癌组织肿瘤坏死因子受体相关因子3(TRAF3)、肿瘤坏死因子受体相关因子6(TRAF6)阳性表达与肿瘤浸润、转移的关系。方法选择胃癌组织及配对癌旁正常组织标本各87份。制作组织芯片,采用免疫组化微阵列技术检测TRAF3、TRAF6表达,分析TRAF3、TRAF6阳性表达与胃癌浸润、转移的关系。结果胃癌组织TRAF3阳性表达率明显低于癌旁正常组织(P<0.01),TRAF6阳性表达高于癌旁正常组织(P<0.05)。TRAF3阳性表达与胃癌组织分化程度、TNM分期、淋巴结转移有关(P均<0.05),TRAF6阳性表达与胃癌组织分化程度、TNM分期有关(P均<0.05)。结论胃癌组织TRAF3阳性表达下调、TRAF6阳性表达上调,其表达变化可能参与胃癌的浸润及转移。     

前列腺癌组织miR-221和miR-222的表达及临床意义

目的探讨前列腺癌(PCa)组织中miR-221和miR-222的表达及其临床意义。方法采用茎环RT-PCR的方法检测8例正常前列腺组织、74例PCa组织及其对应的癌旁组织中miR-221和miR-222的表达情况,并结合临床病理资料进行统计学分析。结果 miR-221和miR-222在PCa组织中的表达显著高于其对应癌旁组织(P<0.05)及正常前列腺组织(P<0.05),其中miR-221的水平还与癌组织分化程度(P<0.05)以及远端转移情况(P<0.05)相关。结论 miR-221和miR-222在PCa组织中表达量升高,其中miR-221的水平还与癌组织分化程度与转移相关,可能作为PCa诊断和预后的指标。     

PTEN/PI3K/AKT蛋白、miR-21在青海藏族及汉族胃癌患者癌组织中的表达差异

目的探讨PTEN/PI3K/AKT蛋白、miR-21在青海藏族及汉族胃癌患者癌组织中的表达差异。方法选取2016年12月至2018年12月我院胃肠外科进行手术的78例胃癌患者为研究对象(藏族38例,汉族40例)。采用实时荧光定量PCR法检测癌组织标本及癌旁组织中miR-21水平,采用免疫组化染色法检测PTEN/PI3K/AKT蛋白,分析不同民族胃癌患者不同组织PTEN/PI3K/AKT蛋白、miR-21表达情况,同时分析其与临床病理特征之间的关系。结果汉族、藏族胃癌患者癌组织中miR-21表达水平高于癌旁组织(P<0.05),且汉族癌组织中miR-21表达水平明显高于藏族(P<0.05)。汉族、藏族胃癌患者癌组织中PTEN、PI3K、AKT蛋白阳性率比较,差异均有统计学意义(P<0.05),其中汉族胃癌组织中PTEN蛋白阳性率低于藏族,但PI3K、AKT蛋白阳性率高于藏族(P<0.05)。两民族胃癌组织中的PTEN、AKT蛋白、miR-21表达与TNM分期、分化程度、淋巴转移相关(P<0.05),PI3K蛋白表达与TNM分期、淋巴转移相关(P<0.05)。在汉族、藏族胃癌患者中,miR-21与PTEN蛋白均呈负相关,与PI3K、AKT蛋白均呈正相关(P<0.05)。结论PTEN在藏族、汉族胃癌患者中表达水平降低,且汉族表达低于藏族,且PTEN与PI3K、AKT呈负相关,miR-21可能通过抑制PTEN,激活PI3K、AKT信号通路从而参与胃癌患者的发生、发展。     

老年胃癌组织miR-183、miR-144-3p表达及其与预后的关系

目的 探讨老年胃癌患者胃癌组织微小RNA-183(miR-183)、miR-144-3p表达水平及其与预后的关系。方法 手术切除治疗的胃癌患者75例采用实时荧光定量聚合酶链反应(qRT-PCR)法检测癌组织及癌旁正常组织中miR-183、miR-144-3p表达水平,分析两者与临床病理特征的关系,采用Kaplan-Meier法进行生存分析,并采用Cox回归分析进行预后影响因素分析。结果 胃癌组织miR-183相对表达量(2.56±0.28)显著高于癌旁正常组织(1.05±0.17);胃癌组织miR-144-3p相对表达量(0.45±0.11)显著低于癌旁正常组织(0.96±0.18,均P<0.05)。胃癌组织miR-183、miR-144-3p表达水平与TNM分期、脉管癌栓、淋巴结转移均显著相关(P<0.05)。Kaplan-Meier生存分析显示,miR-183高表达组总生存(OS)率及无进展生存(DFS)率均显著低于miR-183低表达组,miR-144-3p低表达组OS率及DFS率均显著低于miR-144-3p高表达组(P<0.05)。Cox回归分析显示,淋巴结...     

葡萄糖转运蛋白-1和缺氧诱导因子-1α在胃癌组织中的表达及其与预后的关系

[目的]探讨葡萄糖转运蛋白-1(GLUT-1)、缺氧诱导因子-1α(HIF-1α)在胃癌组织中表达水平与临床病理特征及预后的关系。[方法]选择经我院术后病理确诊并有完整临床资料的胃癌标本80例设为观察组,将其癌旁组织设为对照组。采用免疫组织化学法检测GLUT-1、HIF-1α的表达情况,并分析其与临床病理特征的关系。采用COX回归模型分析预后影响因素。[结果]GLUT-1、HIF-1α在胃癌组织中的表达率(77.50%、73.75%)明显高于在癌旁组织中的表达率(11.25%、16.25%),差异有统计学意义(P<0.05)。胃癌组织中GLUT-1、HIF-1α的表达之间呈正相关(P<0.05)。胃癌组织中GLUT-1阳性表达与TNM分期、淋巴转移均相关(P<0.05),而与患者的年龄、性别、分化程度无明显关系。胃癌组织中HIF-1α阳性表达与肿瘤分化程度、TNM分期、淋巴转移均相关(P<0.05),而与患者的年龄、性别无明显关系。多因素COX回归分析显示GLUT-1、HIF-1α的表达水平是影响胃癌预后的独立危险因素。[结论]GLUT-1、HIF-1α可能与胃癌发生、进展密切相关,对胃癌的诊断、疗效及预后的评估有一定的临床价值。     

miR-618在非小细胞肺癌中的表达及临床意义

目的探讨微小RNA-618(miR-618)在非小细胞肺癌(NSCLC)中的表达水平及临床意义。方法收集2012年1月-2014年1月我院NSCLC患者手术标本85例,采用实时荧光定量PCR(qRT-PCR)检测miR-618在85例NSCLC组织和其配对的癌旁组织中的表达水平,Kaplan-Meier法绘制生存曲线,Log Rank检验分析高低表达水平组的生存率差异。分析miRNA-618的表达水平与NSCLC临床病理参数的关系;多因素Logistic回归分析miRNA-618表达水平的影响因素。结果miRNA-618在NSCLC组织中的表达为(0.78±0.48),显著低于癌旁组织中的表达水平(0.99±0.57)(P<0.05)。miR-618低表达的NSCLC患者的术后5年总生存率为15.22%(7/46),显著低于miR-618高表达的NSCLC患者的33.33%(13/39)。miR-618低表达组中位OS(<28个月),低于miR-618高表达组中位OS(>36个月)(P<0.05)。miR-618在NSCLC组织中的表达水平与患者的年龄、性别、组织类型无关(P>0.05),与肿瘤大小、分化程度、TNM分期和淋巴结转移有关(P<0.05)。Logistic回归显示:肿瘤大小、分化程度、TNM分期和淋巴结转移是miR-618表达的显著影响因素(P<0.05)。结论miRNA-618在NSCLC中表达下调,其表达与NSCLC发生发展、预后有关,miR-618可作为NSCLC诊断和预后预测新的靶点。     

microRNA-101和环氧合酶-2在胃癌中的表达及其临床意义

背景：microRNAs是一类对靶基因表达具有转录后调控作用的非编码小RNA。microRNA-101（miR-101）在多种恶性肿瘤中呈低表达,而过表达外源性miR-101可发挥肿瘤抑制作用。前期体内、外实验发现外源性miR-101可抑制胃癌细胞增殖和环氧合酶-2（COX-2）表达。目的：检测胃癌组织中的miR-101、COX-2表达并探讨其临床意义。方法：收集手术切除胃癌组织和配对癌旁非癌组织标本30例,以实时荧光定量RT—PCR检测miR-101、COX-2mRNA表达,分析两者间以及两者与胃癌主要临床病理特征的关系。结果：绝大多数胃癌组织中miR-101表达低下,COX-2mRNA则呈过表达。胃癌组织与癌旁非癌组织间miR-101、COX-2mRNA表达量差异显著（P〈0．01）,且两者表达在癌组织和癌旁组织中均呈负相关（癌组织：r=-0．767,P=0．000;癌旁组织：r=-0．718,P=0．000）。TNMⅢ、IV期胃癌和伴淋巴结转移的胃癌中,miR-101表达分别显著低于TNMI、Ⅱ期病例和无淋巴结转移病例（P〈0．05）,COX-2mRNA表达分别显著高于TNMI、Ⅱ期病例和无淋巴结转移病例（P〈0．05）。结论：miR-101与COX-2之间的负相关性可能有助于胃癌的临床诊断;miR-101表达低下伴COX-2过表达与胃癌临床进展和转移有关,对预后判断有一定参考价值。     

Transcription factor Sp1 expression in gastric cancer and its relationship to long-term prognosis

AIM: To explore the expression of Spl in gastric carcinoma as well as its association with other clinicopathologic features, and to evaluate the role of Spl as a prognostic indicator of gastric carcinoma.METHODS: By using immunohistochemistry, we examined the Sp1 expression patterns in 65 cases of human gastric cancer, and 40 normal gastric mucosa specimens. Simultaneously, the correlation between Sp1 expression and clinical outcome or clinicopathologic features was investigated.RESULTS: The percentage of Spl expression was 12.5% (5/40) in normal gastric mucosa, and the Sp1 protein was mainly expressed in the nuclei of cells located in the mucous neck region. In sharp contrast, strong Sp1 expression was detected in tumor cells, whereas no or faint Sp1 staining was detected in stromal cells and normal glandular cells surrounding the tumors. The expression rate of Sp1 in gastric cancer lesions was 53.85% (35/65). The medium survival duration in patients who had a tumor with negative, weak and strong Sp1 expressions was 1700, 1560 and 1026d, respectively (P&lt;0.05). Sp1 protein expression was closely related to the depth of tumor infiltration (x^2=13.223, P&lt;0.01) and TNM stage (x^2=11.009, P&lt;0.05), but had no relationship with the number of lymph nodes and Lauren‘s classification (P&gt;0.05). Cox regression model for multivariate analysis revealed that high Spl expression (P&lt;0.05) and advanced stage (P&lt;0.01) were independent predictors of poor survival.CONCLUSION: Normal and malignant gastric tissues have unique Sp1 expression patterns. Spl might serve as an independent prognostic factor, by influencing the tumor infiltration and progression.     

Significance of vascular endothelial growth factor messenger RNA expression in gastric cancer

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miR-1180在胃癌中的表达及其对胃癌增殖、凋亡的影响

目的研究miR-1180在胃腺癌组织及胃癌细胞系中的表达及其对胃癌细胞增殖、凋亡的影响,探讨miR-1180在胃癌中可能的作用机制。方法应用qRT-PCR检测58例胃腺癌及20例癌旁正常组织中miR-1180的表达,分析其表达与胃腺癌临床病理特征的关系。检测miR-1180在胃癌细胞系中的表达,慢病毒干扰技术下调SGC-7901中miR-1180的表达,检测下调后对SGC-7901增殖、凋亡及细胞周期的影响。结果与癌旁正常组织比较,58例胃腺癌组织中miR-1180的表达明显增加(t=16.463,P=0.000),miR-1180的表达与患者的肿瘤大小、TNM分期及淋巴结转移有关(P均<0.05)。miR-1180在胃癌细胞系中表达升高(P=0.000),下调SGC-7901中miR-1180的表达,可见细胞增殖减少(3113±74 vs 1673±51,P=0.000),凋亡增加(4.313±0.220 vs 7.717±0.125,P=0.000);细胞周期G 1期细胞明显增加(45.89±0.33 vs 60.44±0.390,P=0.000),S期细胞明显减少(35.523±0.354 vs 21.953±0.444,P=0.000),G 2期细胞变化不大(18.587±0.672 vs 17.603±0.731,P=0.162)。结论miR-1180的表达促进胃癌的进展,胃癌中miR-1180的高表达与预后不良有关。     

Correlation of microRNA-367 in the clinicopathologic features and prognosis of breast cancer patients

Breast cancer (BC) is a malignant tumor originating from cells of the breast. Notably, microRNAs have been recognized as biomarkers of BC metastasis. The present study is designed to evaluate the association between microRNA (miR)-367 expression and BC with the variance of clinicopathologic features and prognosis.Initially, 63 BC patients were allocated in the BC group, while the other 40 healthy volunteers were recruited as the control group. miR-367 expression in the serum of patients and healthy controls was detected using real-time polymerase chain reaction. Furthermore, the relation between miR-367 in serum and clinicopathologic features and prognosis of BC patients was accessed.miR-367 expression in serum of the BC group was evidently lower than that in the control group (all P < .001). Besides, miR-367 underexpression in the BC group was closely associated with the variance in tumor nodes metastasis advanced stage, tumor diameter, and lymph node metastasis of BC (all P < .001). In addition, compared with the control group, poorly expressed miR-367 BC group had short period of disease-free survival and overall survival (all P < .001).Our study demonstrated that miR-367 expression is associated with BC clinicopathologic features and prognosis. This investigation may offer new insight for BC treatment.     

Hypermethylation of Syk gene in promoter region associated with oncogenesis and metastasis of gastric carcinoma

AIM: To investigate the relationship between methylation of Syk (spleen tyrosine kinase) gene in promoter region and oncogenesis, metastasis of gastric carcinoma. The relation between silencing of the Syk gene and methylation of Syk promoter region was also studied. METHODS: By using methylation-specific PCR (MSP) technique, the methylation of Syk promoter region in specimens from 61 gastric cancer patients (tumor tissues and adjacent normal tissues) was detected. Meanwhile, RT-PCR was used to analyse syk expression exclusively. RESULTS: The expression of the Syk gene was detected in all normal gastric tissues. Syk expression in gastric carcinoma was lower in 14 out of 61 gastric cancer samples than in adjacent normal tissues (chi(2)=72.3, P<0.05). No methylation of Syk promoter was found in adjacent normal tissues. hypermethylation of Syk gene in promoter was detected 21 cases in 61 gastric carcinoma patients. The rate of methylation of Syk promoter in gastric carcinoma was higher than that in adjacent normal tissues (chi(2)=25.1, P<0.05). In 31 patients with lymph node metastasis, 17 were found with Syk promoter methylation. A significant difference was noted between two groups (chi(2)=11.4,P<0.05). CONCLUSION: Hypermethylation leads to silencing of the Syk gene in human gastric carcinoma. Methylation of Syk promoter is correlated to oncogenesis and metastasis of gastric carcinoma. Syk is considered to be a potential tumor suppressor and anti-metastasis gene in human gastric cancer.     

DNA ploidy analysis and expression of MMP-9, TIMP-2, and E-cadherin in gastric carcinoma

AIM: To investigate DNA ploidy and expression of MMP-9, TIMP-2, and E-cadherin in gastric carcinoma and to explore the mechanism of invasion and metastasis of gastric carcinoma. METHODS: Immunohistochemical methods were used to detect the expressions of MMP-9, TIMP-2, and E-cadherin in 156 cases, including 99 cases of gastric carcinoma, 16 cases of adjacent noncancerous mucosa, 16 cases of distant metastases and 25 cases of metastatic lymph node (LN) from gastric carcinoma. Flow cytometry DNA ploidy and S-phase fraction (SPF) analysis were performed on 57 cases, including 47 cases of gastric cancer, 6 cases of adjacent noncancerous mucosa, and 4 cases of distant metastatic cancer. RESULTS: The expression of MMP-9 was significantly correlated with Lauren's classification, Borrmann's classification, LN metastasis, tumor metastasis, and TNM stage, as well as depth of invasion (all P<0.05). The positive rate was lower in noncarcinoma than in carcinoma (31.3% vs66.7%, P<0.01). The expression of TIMP-2 was significantly correlated with Borrmann's classification, LN metastasis, and the depth of invasion (all P<0.05), The expression of E-cadherin was significantly correlated with differentiation, Lauren's classification, Borrmann's classification, and LN metastasis, as well as the depth of invasion (P<0,01 or P<0.05). E-cadherin was less expressed in carcinoma than in noncarcinoma (42.4% vs87.5%, P<0.01). There was a positive correlation between MMP-9 and TIMP-2 and a negative correlation between MMP-9 and E-cadherin, but no correlation between TIMP-2 and E-cadherin. Also there was a positive correlation between DNA aneuploid rate and differentiation and LN metastasis. SPF that was higher than 15% was positively correlated with tumor size, differentiation and LN metastasis. And there was a significant difference between carcinoma and noncarcinoma in DNA aneuploid rate and SPF. CONCLUSION: With tumor progression and development of heterogeneity, the abnormal expressions of MMP-9, TIMP-2, and E-cadherin or DNA aneuploid rate or high SPF gradually increases, suggesting that they play a crucial role in gastric carcinoma progression.