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1.
Salvatore Sauro Ashvin Babbar Borzo Gharibi Victor Pinheiro Feitosa Ricardo Marins Carvalho Lidiany Karla Azevedo Rodrigues Avijit Banerjee Timothy Watson 《Dental materials》2018,34(6):868-878
Objective
Materials for pulp protection should have therapeutic properties in order to stimulate remineralization and pulp reparative processes. The aim of this study was to evaluate the mechanical properties, biocompatibility, cell differentiation and bioactivity of experimental light-curable resin-based materials containing bioactive micro-fillers.Methods
Four calcium-phosphosilicate micro-fillers were prepared and incorporated into a resin blend: 1) Bioglass 45S5 (BAG); 2) zinc-doped bioglass (BAG-Zn); 3) βTCP-modified calcium silicate (β-CS); 4) zinc-doped β-CS (β-CS-Zn). These experimental resins were tested for flexural strength (FS) and fracture toughness (FT) after 24 h and 30-day storage in simulated body fluid (SBF). Cytotoxicity was evaluated using MTT assay, while bioactivity was evaluated using mineralization and gene expression assays (Runx-2 & ALP).Results
The lowest FS and FT at 24 h was attained with β-CS resin, while all the other tested materials exhibited a decrease in FS after prolonged storage in SBF. β-CS-Zn maintained a stable FT after 30-day SBF aging. Incorporation of bioactive micro-fillers had no negative effect on the biocompatibility of the experimental materials tested in this study. The inclusion of zinc-doped fillers significantly increased the cellular remineralization potential and expression of the osteogenic genes Runx2 and ALP (p < 0.05).Significance
The innovative materials tested in this study, in particular those containing β-CS-Zn and BAG-Zn may promote cell differentiation and mineralization. Thus, these materials might represent suitable therapeutic pulp protection materials for minimally invasive and atraumatic restorative treatments. 相似文献2.
Objectives
This study investigated the effect of matrix metalloproteinase 8 (MMP-8) on resin–dentin bonds, assessed the mechanical properties of the interfaces over time, and discussed the potential application of MMP-8 inhibitor I (MMP8-I) as a specific MMP-8 inhibitor to be incorporated into dental adhesives.Methods
The activation and inhibition of MMP-8 was detected by colorimetric assay. After 1 day, 6 months and 1 year of storage of Control, MMP8-I, and chlorhexidine (CHX) groups, the microtensile bond strengths (μTBS) were used to evaluate the bond strength and failure mode distributions, and nanoleakage analysis was used to evaluate the minor scattered silver particles.Results
Colorimetric assay showed that the activated MMP-8 was enhanced by adhesive procedures, while it was inhibited by the additional treatment of MMP8-I or CHX. Compared with the Control and CHX groups, the MMP8-I group had significantly higher bond strength and the hybrid layer from the MMP8-I-treated dentin exhibited structural integrity of the collagen network and decreased silver nitrate penetration after 1 year of storage.Significance
MMP-8 inhibition I protects against the degradation of resin–dentin bonds over time, which is better than broad-scale enzyme inhibitor CHX. It shows that MMP8-I may be used in dentistry for preventing collagen degradation within hybrid layers to extend the longevity of resin–dentin bonds. 相似文献3.
Noriko Hiraishi Takahiro Maruno Naoya Tochio Ryohei Sono Masayuki Otsuki Tsutomu Takatsuka Junji Tagami Yuji Kobayashi 《Dental materials》2017,33(1):33-42
Objective
Dentin collagen can be modified by some plant-derived flavonoids to improve properties of dentin organic matrix. Hesperidin (HPN), a hesperetin-7-O-rutinoside flavonoid, has a potential of dentin modification for being based on evidence that a treatment with HPN may resist collagenase degradation and arrest demineralization of human dentin. In this study, biophysical and molecular-level information on the interaction of HPN and collagen was investigated.Methods
HPN is extracted from citrus fruits. Sample collagenous solution was prepared using atelocollagen (ATCL) as a triple-helical peptide model. We have performed circular dichroism spectroscopic analysis, sedimentation velocity measurement by ultracentrifuge and saturation transfer difference measurement (STD) by NMR on HPN-collagen in solution state.Results
The circular dichroism and sedimentation velocity measurement showed the evidence for the molecular interaction between ATCL and HPN, while HPN did not induce any conformational change of ATCL. The STD-NMR study further confirmed this interaction and suggested that HPN interacted with ATCL through its aromatic part, not through its disaccharide moiety.Significance
These findings indicated that HPN is weakly bound to ATCL not causing structural modification of collagen. This interaction may contribute to the preservation of collagen by protecting from collagenase degradation. 相似文献4.
Background
Promising results of initial clinical trials with yttria-stabilized zirconia have led to more extensive use of zirconia in dental implant superstructures. The applications have extended to abutments and complex individually designed crown-abutment one-piece structures. Little is known about their clinical success and the primary cause of failures.Purpose
The aim of this study was to identify the cause of fracture of retrieved implant-retained one-piece prostheses that failed during clinical use.Methods
Nine fractured restorations were analyzed with fractographic methods and their fracture origins were identified.Results
All but two of the fractures originated in an area of tight contact between the implant or titanium screw and the abutment base. Results of the evaluation showed that zirconia-based implant restorations with very thin walls in the region connecting the prosthesis to the implant are vulnerable to damage from the screw retaining process and fracture from non-axial loads. Two restorations failed due to veneer fractures.Significance
The findings suggest that large crowns on narrow implants or implants with internal fixation should preferably not be made with zirconia abutments, or that a new design approach should be considered. 相似文献5.
Saad B. Qasim Shariq Najeeb Robin M. Delaine-Smith Andrew Rawlinson Ihtesham Ur Rehman 《Dental materials》2017,33(1):71-83
Objective
The regeneration of periodontal tissues lost as a consequence of destructive periodontal disease remains a challenge for clinicians. Guided tissue regeneration (GTR) has emerged as the most widely practiced regenerative procedure. Aim of this study was to electrospin chitosan (CH) membranes with a low or high degree of fiber orientation and examines their suitability for use as a surface layer in GTR membranes, which can ease integration with the periodontal tissue by controlling the direction of cell growth.Methods
A solution of CH-doped with polyethylene oxide (PEO) (ratio 95:5) was prepared for electrospinning. Characterization was performed for biophysiochemical and mechanical properties by means of scanning electron microscopy (SEM), Fourier Transform Infrared (FTIR) spectroscopy, swelling ratio, tensile testing and monitoring degradation using pH analysis, weight profile, ultraviolet–visible (UV–vis) spectroscopy and FTIR analysis. Obtained fibers were also assessed for viability and matrix deposition using human osteosarcoma (MG63) and human embryonic stem cell-derived mesenchymal progenitor (hES-MP) cells.Results
Random and aligned CH fibers were obtained. FTIR analysis showed neat CH spectral profile before and after electrospinning. Electropsun mats were conducive to cellular attachment and viability increased with time. The fibers supported matrix deposition by hES-MPs. Histological sections showed cellular infiltration as well.Significance
The surface layer would act as seal to prevent junctional epithelium from falling into the defect site and hence maintain space for bone regeneration. 相似文献6.
Ya-Hui Chan Wei-Zhen Lew Emily Lu Thomas Loretz Luke Lu Che-Tong Lin Sheng-Wei Feng 《Dental materials》2018,34(3):470-485
Objectives
The aim of this study was to evaluate the in vitro biocompatibility and in vivo osseointegration of three novel bioactive glass fiber reinforced composite (GFRC) implants and to compare these with metal (Ti6Al4V) implants.Methods
The surfaces of these experimental substrates were characterized by scanning electron microscopy (SEM), a 2D profilometer and by contact angle measurement. In vitro biological performance was assessed using MG-63 human osteoblast-like cell morphology, cell proliferation assays and the alkaline phosphatase (ALP) activity testing. Furthermore, in vivo osseointegration performance was examined by installing samples into rabbit femurs and evaluated the results using micro-CT, histology and histomorphometrical analysis; these assessments were carried out after 1, 2, 4 and 8 weeks of healing.Results
The results showed that moderate surface roughness, moderate hydrophilic exposure and moderate homogenous exposure of bioactive glass fibers were present for all of the GFRC substrates. Furthermore, MG-63 cells, when cultured on all of the GFRC substrates, grew well and exhibited a more differentiated phenotype than cells grown on titanium alloy (Ti6Al4V) substrate. Histological evaluation revealed more newly-formed bone regeneration within the thread of the GFRC implants during the initial healing period. In addition, the novel GFRC implants with a bioactive Bio-fiber structure and glass particles within the epoxy resin matrix showed better bone volume/tissue volume (BV/TV) values at 4 weeks and this was accompanied by bone-implant contact (BIC) values at 8 weeks comparable to the Ti6Al4V group.Significance
These findings demonstrated that novel GFRC implants seem to show improved osteogenesis and osseointegration functionality and have potential as a substitute for Ti6Al4V, or other metal-based materials, when used for clinically dental and orthopedic applications. 相似文献7.
M. Elashiry M.M. Meghil S. Kalathingal A. Buchanan R. Elrefai S. Looney M. Rajendran M. Ochieng N. Young A. Elawady R.M. Arce K.H. Sandhage C.W Cutler 《Dental materials》2018,34(4):619-628
Background
Periodontitis is an infectious/inflammatory disease most often diagnosed by deepening of the gingival sulcus, which leads to periodontal pockets (PPs) conventional manual periodontal probing does not provide detailed information on the three-dimensional (3-D) nature of PPs.Objectives
To determine whether accurate 3-D analyses of the depths and volumes of calibrated PP analogues (PPAs) can be obtained by conventional cone beam computed tomography (CBCT) coupled with novel radiopaque micro-particle fillers (described in the companion paper) injected into the PPAs.Methods
Two PPA models were employed: (1) a human skull model with artificial gingiva applied to teeth with alveolar bone loss and calibrated PPAs, and (2) a pig jaw model with alveolar bone loss and surgically-induced PPAs The PPAs were filled with controlled amounts of radiopaque micro-particle filler using volumetric pipetting Inter-method and intra-method agreement tests were then used to compare the PPA depths and volumes obtained from CBCT images with values obtained by masked examiners using calibrated manual methods.Results
Significant inter-method agreement (0.938–0.991) and intra-method agreement (0.94–0.99) were obtained when comparing analog manual data to digital CBCT measurements enabled by the radiopaque filler.Significance
CBCT imaging with radiopaque micro-particle fillers is a plausible means of visualizing and digitally assessing the depths, volumes, and 3-D shapes of PPs This approach could transform the diagnosis and treatment planning of periodontal disease, with particular initial utility in complex cases Efforts to confirm the clinical practicality of these fillers are currently in progress. 相似文献8.
Stefano Pieralli Ralf-Joachim Kohal Emilia Lopez Hernandez Sam Doerken Benedikt Christopher Spies 《Dental materials》2018,34(2):171-182
Objective
To determine the osseointegration rate of zirconium dioxide (ZrO2) dental implants in preclinical investigations.Data
Data on the osseointegration rate was extracted considering the bone to implant contact (BIC), removal torque analysis (RTQ) and push-in tests. Meta analyses were conducted using multilevel multivariable mixed-effects linear regression models. The ?idák method was used in case of multiple testing.Sources
An electronic screening of the literature (MEDLINE/Pubmed, Cochrane Library and Embase) and a supplementary manual search were performed. Animal investigations with a minimum sample size of 3 units evaluating implants made of zirconia (ZrO2) or its composites (ZrO2 > 50 vol.%) were included.Study selection
The search provided 4577 articles, and finally 54 investigations were included and analyzed. Fifty-two studies included implants made from zirconia, 4 zirconia composite implants and 37 titanium implants. In total, 3435 implants were installed in 954 animals.Conclusions
No significant influence of the evaluated bulk materials on the outcomes of interest could be detected. When comparing different animal models, significant differences for the evaluated variables could be found. These results might be of interest for the design of further animal investigations. 相似文献9.
Biodentine? is a novel tricalcium silicate based material used both as a coronal dentine replacement and in pulp therapy. Its multiple use in sealing perforations, pulp capping and as a temporary restoration arises from its ability to promote dentine formation and to confer an excellent marginal seal. However, there is still room for improvement of this cement as it lacks the anticariogenic effect typically conferred by fluoride ion release as seen in glass ionomer cement based dental materials. Therefore, this study was conducted to investigate the impact of bioactive glass addition to Biodentine?.
Objective
was to compare the apatite formation capacity, specificity of the apatite type formed and fluoride ion release by Biodentine? cements that have been modified by three different compositions of bioactive glasses.Methods
High fluoride, high strontium and high fluoride plus strontium containing bioactive glasses were synthesized, incorporated into Biodentine? powder and four types of cements prepared. These cements were immersed in phosphate buffered saline solution and incubated for a period of 3 and 24 h, 3, 7 and 14 days. Fourier transform infra-red spectroscopy, X-ray diffraction, magic angle spinning nuclear magnetic resonance and fluoride ion release studies were performed.Results
Bioactive glass addition to Biodentine? led to pronounced formation of apatite. Where the bioactive glass contained fluoride, fluorapatite and fluoride ion release were demonstrated.Significance
Eliciting fluorapatite formation and fluoride ion release from Biodentine? is an important development as fluoride is known to have antibacterial and anticariogenic effects. 相似文献10.
H. Xia P. Picart S. Montresor R. Guo J.C. Li O. Yusuf Solieman J.-C. Durand M. Fages 《Dental materials》2018,34(8):1222-1234
Objectives
CAD/CAM ceramic occlusal veneers are increasingly used as therapeutic options. However, little is known about their mechanical behavior under stress, as the response of the prepared tooth that supports it. The aim of this article is to use for the first time 3D color holography to evaluate the behavior of a molar occlusal veneer under stress and the response of the prepared tooth.Methods
The occlusal surface of a lower molar is prepared to receive a specific monolithic ceramic reconstruction manufactured with a chairside CAD/CAM system. Longitudinally cut samples are used to get a planar object observation and to “look inside” the tooth. A digital holographic set-up permits to obtain the contact-less and one-shot measurement of the three-dimensional displacement field at the surface of the tooth sample; stain fields are evaluated with low noise-sensitive computation.Results
Figures show the strain fields with micro-strain units and highlight the behavior of the ROI (region of interest) in the three directions of space. The ROI are: the ceramic, the glue junction, the dentin enamel junction, dentin and enamel. The results show an excellent behavior of the restored tooth without areas of excessive stress concentrations, but also a significant involvement of the dentin enamel junction.Significance
The ceramic occlusal veneer seems to behave in accordance with the biomechanical concepts ensuring the longevity of the reconstituted tooth. 3D holography is a highly recommended method for studying dental biomechanics. 相似文献11.
Anna Poimenova Efthymia Kitraki Afroditi Kakaboura Christos Rahiotis 《Dental materials》2018,34(4):e73-e82
Objective
Early responses of human pulp to Prime&Bond/phosphoric acid, Clearfil SE Bond, Clearfil S3 Bond and Dycal were investigated ex vivo.Materials and methods
The three adhesives, Dycal or buffer (DPBS) were applied directly onto the pulp of human teeth slices that were placed in culture for 4 days. Cell viability was monitored by the MTT assay during the culture period. After 4 days, tissue integrity was examined by hematoxylin–eosin staining. Vimentin levels were assessed by Western blotting. TUNEL assay was applied for apoptotic cell detection at specific pulp areas.Results
Profound reduction of cell viability and tissue integrity was observed in adhesive-treated groups, while the impact of Dycal was found to be less harmful. Extended apoptosis was caused mostly by the Clearfil SE and Prime&Bond. All adhesives reduced Vimentin levels.Significance
The study provides evidence that early pulp responses to direct capping with different adhesive systems or calcium hydroxide may vary significantly and underline the need for further studies in relevant ex vivo systems. 相似文献12.
Abdul Manaf Abdullah Tuan Noraihan Azila Tuan Rahim Wan Nur Fadilla Wan Hamad Dasmawati Mohamad Hazizan Md Akil Zainul Ahmad Rajion 《Dental materials》2018,34(11):e309-e316
Objective
To compare the mechanical and biological properties of newly developed hybrid ceramics filled and unfilled polyamide 12 (PA 12) for craniofacial reconstruction via a fused deposition modelling (FDM) framework.Methods
15 wt% of zirconia (ZrO2) as well as 30, 35, and 40 wt% of beta-tricalcium phosphate (β-TCP) were compounded with PA 12, followed by the fabrication of filament feedstocks using a single screw extruder. The fabricated filament feedstocks were used to print the impact specimens. The melt flow rate, tensile properties of fabricated filament feedstocks, and 3D printed impact properties of the specimens were assessed using melt flow indexer, universal testing machine, and Izod pendulum tester, respectively. The microstructure of selected filament feedstocks and broken impact specimens were analysed using a field emission scanning electron microscope and universal testing machine. Human periodontal ligament fibroblast cells (HPdLF) were used to evaluate the cytotoxicity of the materials by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromid) (MTT) assay.Results
Hybrid ceramics filled PA 12 indicated sufficient flowability for FDM 3D printing. The tensile strength of hybrid ceramics filled PA 12 filament feedstocks slightly reduced as compared to unfilled PA 12. However, the tensile modulus and impact strength of hybrid ceramics filled PA 12 increased by 8%–31% and 98%–181%, respectively. A significant increase was also detected in the cell viability of the developed composites at concentrations of 12.5, 25, 50 and 100 mg/ml.Significance
The newly developed hybrid ceramics filled PA 12 filament feedstock with improved properties is suitable for an FDM-based 3D printer, which enables the creation of patient-specific craniofacial implant at a lower cost to serve low-income patients. 相似文献13.
Amanda Maria de Oliveira Dal Piva João Paulo Mendes Tribst Alexandre Luiz Souto Borges Rodrigo Othávio de Assunção e Souza Marco Antonio Bottino 《Dental materials》2018,34(9):1342-1350
Objectives
To investigate the influence of different materials for monolithic full posterior crowns using 3D-Finite Element Analysis (FEA).Methods
Twelve (12) 3D models of adhesively-restored teeth with different crowns according to the material and its elastic modulus were analysed: Acrylic resin, Polyetheretherketone, Composite resin, Hybrid ceramic, pressable and machinable Zirconia reinforced lithium silicate, Feldspathic, Lithium disilicate, Gold alloy, Cobalt–Chromium alloy (Co–Cr), Zirconia tetragonal partially stabilized with yttria, and Alumina. All materials were assumed to behave elastically throughout the entire deformation. Results in restoration and cementing line were obtained using maximum principal stress. In addition, maximum shear stress criteria was used for the cementing line.Results
Restorative materials with higher elastic modulus present higher stress concentration inside the crown, mainly tensile stress on an intaglio surface. On the other hand, materials with lower elastic modulus allow stress passage for cement, increasing shear stress on this layer. Stiffer materials promote higher stress peak values.Significance
Materials with higher elastic modulus such as Co–Cr, zirconia and alumina enable higher tensile stress concentration on the crown intaglio surface and higher shear stress on the cement layer, facilitating crown debonding. 相似文献14.
Luca Boschini 《Giornale Italiano di Endodonzia》2018,32(2):76-79
Aim
As dental trauma is an unpredictable event, the patient is sometimes unable to receive dental care immediately in case of tooth fracture complicated by exposure to the pulp. It is conceivable that a long wait may favor bacterial contamination that can lead to necrosis of the pulp. The aim of this paper is to present a clinical case in which the pulp has been protected domiciliary to reduce post-traumatic hypersensitivity and the risk of pulpal necrosis.Materials and methods
In the presented clinical case, the nail polish was used as an emergency material for the direct capping of the pulp of two fractured incisors. In this way it was possible to protect the pulp and seal the dentinal tubules for 5 days, such it is the time between trauma and therapy.Results
The application of the nail polish led to a reduction in hypersensitivity; at 2-year follow-up both teeth were still vital.Conclusions
Based on this experience, it is suggested the possibility to recommend the use of nail polish as a protective material for the pulp and for reducing symptoms in case of complicated and uncomplicated fractures, if the dentist is consulted by telephone and the patient is not in condition to reach it quickly. 相似文献15.
Lorenzo Breschi Tatjana Maravic Sandra Ribeiro Cunha Allegra Comba Milena Cadenaro Leo Tjäderhane David H. Pashley Franklin R. Tay Annalisa Mazzoni 《Dental materials》2018,34(1):78-96
Objectives
Efforts towards achieving durable resin–dentin bonds have been made for decades, including the understanding of the mechanisms underlying hybrid layer (HL) degradation, manufacturing of improved adhesive systems, as well as developing strategies for the preservation of the HL.Methods
This study critically discusses the available peer-reviewed research concerning the formation and preservation of the HL, the mechanisms that lead to the degradation of the HL as well as the strategies to prevent it.Results
The degradation of the HL occurs through two main mechanisms: the enzymatic degradation of its collagen fibrils, and the leaching of the resin from the HL. They are enabled by residual unbound water between the denuded collagen fibrils, trapped at the bottom of the HL. Consequently, endogenous dentinal enzymes, such as the matrix metalloproteinases (MMPs) and cysteine cathepsins are activated and can degrade the denuded collagen matrix. Strategies for the preservation of the HL over time have been developed, and they entail the removal of the unbound water from the gaps between the collagen fibrils as well as different modes of silencing endogenous enzymatic activity.Significance
Although there are many more hurdles to be crossed in the field of adhesive dentistry, impressive progress has been achieved so far, and the vast amount of available research on the topic is an indicator of the importance of this matter and of the great efforts of researchers and dental material companies to reach a new level in the quality and longevity of resin–dentin bonds. 相似文献16.
Neha Saxena Maegan A. Cremer Evan S. Dolling Hamid Nurrohman Stefan Habelitz Grayson W. Marshall Laurie B. Gower 《Dental materials》2018,34(9):1378-1390
Objective
The polymer-induced liquid-precursor (PILP) mineralization process has been shown to remineralize artificial dentin lesions to levels consistent with those of native dentin. However, nanoindentation revealed that the moduli of those remineralized lesions were only ～50% that of native dentin. We hypothesize that this may be due to the PILP process having been previously optimized to obtain high amounts (～70 wt%) of intrafibrillar crystals, but without sufficient interfibrillar mineral, another significant component of dentin.Methods
Fluoride was added to the PILP-mineralization of collagen from rat tail tendon at varying concentrations to determine if a better balance of intra- versus inter-fibrillar mineralization could be obtained, as determined by electron microscopy. Nanoindentation was used to determine if fluoridated apatite could improve the mechanical properties of the composites.Results
Fluoride was successfully incorporated into the PILP-mineralization of rat tail tendon and resulted in collagen-mineral composite systems with the mineral phase of hydroxyapatite containing various levels of fluoridation. As the fluoride concentration increased, the crystals became larger and more rod-like, with an increasing tendency to form on the fibril surfaces rather than the interior. Nanomechanical testing of the mineralized tendons revealed that fluoride addition did not increase modulus over PILP mineralization alone. This likely resulted from the separated nature of collagen fibrils that comprise tendon, which does not provide lateral reinforcement and therefore may not be suited for the compressive loads of nanoindentation.Significance
This work contributes to the development of minimally invasive approaches to caries treatment by determining if collagen can be functionally mineralized. 相似文献17.
Athina Bakopoulou Αnthie Georgopoulou Ioannis Grivas Chryssa Bekiari Oleg Prymak Κateryna Loza Matthias Epple George C. Papadopoulos Petros Koidis Μaria Chatzinikolaidou 《Dental materials》2019,35(2):310-327
Objective
Biomimetic chitosan/gelatin (CS/Gel) scaffolds have attracted great interest in tissue engineering of several tissues. However, limited information exists regarding the potential of combining CS/Gel scaffolds with oral cells, such as dental pulp stem cells (DPSCs), to produce customized constructs targeting alveolar/orofacial bone reconstruction, which has been the aim of the present study.Methods
Two scaffold types, designated as CS/Gel-0.1 and CS/Gel-1, were fabricated using 0.1 and 1% (v/v) respectively of the crosslinker glutaraldehyde (GTA). Scaffolds (n = 240) were seeded with DPSCs with/without pre-exposure to recombinant human BMP-2. In vitro assessment included DPSCs characterization (flow cytometry), evaluation of viability/proliferation (live/dead staining, metabolic-based tests), osteo/odontogenic gene expression analysis (qRT-PCR) and structural/chemical characterization (scanning electron microscopy, SEM; energy dispersive X-ray spectroscopy, EDX; X-ray powder diffraction, XRD; thermogravimetry, TG). In vivo assessment included implantation of DPSC-seeded scaffolds in immunocompromised mice, followed by histology and SEM-EDX. Statistical analysis employed one/two-way ANOVA and Tukey’s post-hoc tests (significance for p < 0.05).Results
Both scaffolds supported cell viability/proliferation over 14 days in culture, showing extensive formation of a hydroxyapatite-rich nanocrystalline calcium phosphate phase. Differential expression patterns indicated GTA concentration to significantly affect the expression of osteo/odontogenic genes, with CS/Gel-0.1 scaffolds being more effective in upregulating DSPP, IBSP and Osterix. In vivo analysis demonstrated time-dependent production of a nanocrystalline, mineralized matrix at 6, 8 and 10 weeks, being more prominent in constructs bearing rhBMP-2 pre-treated cells. The latter showed higher amounts of osteoid and fully mineralized bone, as well as empty space reduction.Significance
These results reveal a promising strategy for orofacial bone tissue engineering. 相似文献18.
Akinari Inui Ryohei Itou Toshirou Oyama Yoshihiro Tamura Kosei Kubota Wataru Kobayashi 《Oral Science International》2017,14(2):37-39
Aims
To compare the clinical characteristics of sialolithiasis between pediatric and adult patients.Settings and Design
Subject characteristics, clinical manifestations, salivary calculi characteristics, and treatment modalities and outcomes from medical records were retrospectively compared between pediatric and adult patients with sialolithiasis visiting the Department of Oral and Maxillofacial Surgery, Hirosaki University Hospital, between 2005 and 2014.Subjects
We included 5 pediatric (all boys) and 45 adult (20 men; 25 women) patients.Results
Most patients had submandibular swelling. The calculi were located on the right in 100.0% of pediatric patients and 57.8% of adult patients. Moreover, 20.0% of pediatric patients and 35.6% of adult patients exhibited sialoliths in the glands. Calculi were <5 mm in size in 100.0% of pediatric patients and 33.3% of adult patients. Pediatric sialolithiasis treatment included intraoral retrieval under local anesthesia in 4 cases (80.0%) and spontaneous expulsion from the duct in 1 case (20.0%). Adult sialolithiasis treatment included gland resection under general anesthesia in 8 cases (17.8%), intraoral retrieval under general anesthesia in 6 cases (13.3%), intraoral retrieval under local anesthesia in 19 cases (42.2%), spontaneous expulsion in 6 cases (13.3%), and follow-up only in 6 cases (13.3%).Conclusions
A large number of relatively small and distal sialoliths in pediatric patients was removed using intraoral retrieval under local anesthesia. This difference in the choice of treatment between pediatric and adult sialolithiasis may be attributed to the size and palpability of the calculi. 相似文献19.
Yasaman Delaviz Mitchell A. Nascimento Michael W. Laschuk Timothy W. Liu Meilin Yang J. Paul Santerre 《Dental materials》2018,34(5):711-725
Objective
Two leading causes contributing to dental restoration replacement are the marginal breakdown at the composite/dentin interface and secondary caries mediated by bacteria. The objective of the present study was to synthesize oligomers which incorporated enhanced bio-stability but would also be able to generate antimicrobial function if they underwent degradation.Methods
Stability was incorporated into the oligomers by generating structural features that would physically hinder the availability of hydrolytically sensitive groups in the oligomers. As a proof-of concept for the antibacterial feature, antimicrobial function was achieved by covalently incorporating Ciprofloxacin (CF) into the backbone of cross-linking divinyl oligomers (referred to as EDV and HLH-CFPEG). The hydrolytic stability of the oligomers was studied in simulated human salivary esterase and compared to the commercial monomer 2,2-bis[4(2-hydroxy-3-methacryloxypropoxy)-phenyl]propane (BisGMA).Results
Both drug oligomers were found to be significantly more stable than BisGMA. Upon degradation, both drug oligomers released CF differentially in free form. Polymer synthesis from resin formulations containing 15 wt% HLH-CFPEG showed a high degree of vinyl group conversion and gel content, and under hydrolytic conditions showed the release of CF during a 28-day monitoring study period.Significance
HLH-CFPEG can be used in dental resin adhesive systems for local delivery of CF to the marginal interface. Minimizing the growth of Streptococcus mutans at the marginal site can improve longevity by reducing esterase activity derived specifically from S. mutans. 相似文献20.
Midori Nakamura Yuko Nakamichi Toshihide Mizoguchi Masanori Koide Teruhito Yamashita Toshiaki Ara Hiroshi Nakamura Josef M. Penninger Yuriko Furuya Hisataka Yasuda Nobuyuki Udagawa 《Journal of oral biosciences / JAOB, Japanese Association for Oral Biology》2017,59(3):146-151