雾化吸入布地奈德治疗特发性肺纤维化31例临床分析

目的观察布地奈德雾化吸入治疗特发性肺纤维化（IPF）的疗效和不良反应。方法选择我院收治的IPF患者62例,随机分为观察组和对照组各31例,观察组给予布地奈德雾化液1mg,3次／d,4周后改为布地奈德1mg,2冼／d,用8周。对照组给予泼尼松0．50rag／kg口服,1：k．／d,4周后改为泼尼松0．25mg／kg,口服,用8周,两组患者疗程均为3个月。两组患者的氧疗、化痰等基础治疗相同。比较治疗前、后患者的呼吸困难评分、肺功能、动脉血气分析以及空腹血糖、骨质疏松度等。结果两组患者治疗前及治疗后呼吸困难评分、肺功能、血气分析间差异均无统计学意义（P〉0．05）。两组治疗前与治疗后的空腹血糖、骨质疏松度差异均有统计学意义（P〈0．05）。结论布地奈德雾化吸入是治疗特发性肺纤维化的有益探索,可以用其代替口服糖皮质激素。     

瓣膜性心脏病伴慢性心房颤动患者心房组织呼吸链相关蛋白质定量蛋白质组学分析

目的 从蛋白质组学角度分析心房颤动（房颤）患者心房能量代谢状态,揭示房颤的发生基质.方法 2011年1月至8月于中南大学湘雅二医院获取患二尖瓣疾病需行外科换瓣手术的窦性心律患者（n=6）和房颤患者（n=8）左心耳组织.分离蛋白质,行同重元素标记的相对和绝对定量（iTRAQ）标记及质谱分析,通过分析软件行蛋白质鉴定和定量分析,运用蛋白印迹法分析确定结果准确性.结果 发现1 023个蛋白质,517个差异表达蛋白质,122个能量代谢相关,46个参与呼吸链过程.下调蛋白质在慢性房颤患者左心耳组织的能量代谢中占据主导优势.蛋白印迹分析证实了结果的可靠性.结论 瓣膜性心脏病患者慢性房颤过程心房肌细胞产能状态受损.     

基于iTRAQ的定量蛋白质组学技术探索 淡色库蚊越冬机制

目的　比较淡色库蚊越冬后与滞育准备阶段蛋白表达差异,探索淡色库蚊越冬休眠（滞育）的机制。方法　采用同位素标记相对和绝对定量（iTRAQ）技术,对越冬滞育及准备阶段淡色库蚊进行定量蛋白质组学分析。结果　淡色库蚊越冬滞育前后共鉴定出244种差异表达蛋白,其中上调蛋白126种、下调蛋白118种。生物信息学分析表明,这些差异表达蛋白与能量产生及转化、脂代谢、细胞骨架重塑、糖代谢、蛋白质转运、分子伴侣、应激耐受以及各种代谢酶等有关。结论　本研究首次采用现代蛋白质组学工具鉴定淡色库蚊越冬滞育相关蛋白,初步揭示了与淡色库蚊越冬滞育相关的KEGG通路及蛋白,进一步扩展了对蚊媒越冬滞育机制的理解。     

Comparative physiological and proteomic analyses reveal the actions of melatonin in the reduction of oxidative stress in Bermuda grass (Cynodon dactylon (L). Pers.)

The fact of melatonin as an important antioxidant in animals led plant researchers to speculate that melatonin also acts in the similar manner in plants. Although melatonin has significant effects on alleviating stress‐triggered reactive oxygen species (ROS), the involvement of melatonin in direct oxidative stress and the underlying physiological and molecular mechanisms remain unclear in plants. In this study, we found that exogenous melatonin significantly alleviated hydrogen peroxide (H₂O₂)‐modulated plant growth, cell damage, and ROS accumulation in Bermuda grass. Additionally, 76 proteins significantly influenced by melatonin during mock or H₂O₂ treatment were identified by gel‐free proteomics using iTRAQ (isobaric tags for relative and absolute quantitation). Metabolic pathway analysis showed that several pathways were markedly enhanced by melatonin and H₂O₂ treatments, including polyamine metabolism, ribosome pathway, major carbohydrate metabolism, photosynthesis, redox, and amino acid metabolism. Taken together, this study provides more comprehensive insights into the physiological and molecular mechanisms of melatonin in Bermuda grass responses to direct oxidative stress. This may relate to the activation of antioxidants, modulation of metabolic pathways, and extensive proteome reprograming.     

利用蛋白组学解析淡色库蚊对氯氰菊酯的抗性机制

目的 比较氯氰菊酯选育后抗性淡色库蚊与敏感淡色库蚊蛋白差异表达情况,揭示淡色库蚊抗氯氰菊酯杀虫剂的机制.方法 利用同位素相对和绝对定量(iTRAQ)技术,结合液相色谱/串联质谱(LC-MS/MS)分析技术,对氯氰菊酯敏感与抗性淡色库蚊进行蛋白组定量分析.结果 共鉴定出164种蛋白在氯氰菊酯抗性选育前后差异表达,其中上调...