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<正>根据有关读者举报并经本刊初步查证,近一段时间来有人冒充本刊名义和盗用本刊的合法刊号(ISSN1674-3806/CN45-1365/R)进行非法出版活动(该非法出版物的编辑部地址为:北京市100036信箱27分箱;邮政编号:100036;联系电话:010-87013678;网址:http://www.zglcxyx010.com;E-mail:zglcxyx010@126.com、ZGLCXYX@163.com),严重地侵犯本     

中华心血管病杂志2005年度继续教育园地思考多选题答案

第1期急性冠状动脉综合征的概念演化与治疗策略更新:1.A、B、C、D;2.A、D;3.A、B。第2期低分子肝素与血栓栓塞性疾病:1.A、B、C;2.B、C;3.D;4·A、C、D。第3期神经内分泌拮抗剂在慢性心力衰竭治疗中的常见问题:1.B;2.D;3.A;4.D。第4期心率与心血管病危险性:1.B;2.A;3.C;4.D。第5期神经内分泌拮抗治疗慢性心力衰竭的几个热点问题:1.A;2.C;3.E。第6期肾上腺髓质素与心血管病研究现状:1.B;2.C;3.D;4.D。第7期对中国2004年、欧洲2003年高血压防治指南和JNC7血压分类的比较及评价:1.B;2.C;3.B。第8期心肾综合征研究进展:1.B;2.A…     

2012年第二届亚太地区幽门螺杆菌会议

会议主题:科学向临床医学的转化:亚太地区新千年幽门螺杆菌感染特别工作组:早期胃癌的内镜诊断和治疗;H.pylori的微生物学、免疫学和分子遗传学会议热点:H.pylori与胃癌;H.pylori的分子流行病学;H.pylori的最佳治疗方法;H.pylori感染中具有争议的问题:胃食管反流病,消化不良,NSAID肠病以及胃外疾病。时间:2012年1月13～15日     

慢性肾衰血液透析患者血浆儿茶酚胺水平及其心率变异性分析

目的 :观察心透患者血浆儿茶酚胺水平与心率变异性 ,并探讨两者的相关性。方法 :测定慢性肾衰血透患者血浆儿茶酚胺水平及记录 2 4h动态心电图 ,分析其心率变异性。结果 :慢性肾衰血液透析患者血浆多巴胺和去甲肾上腺素水平较对照组明显升高 （分别为 P <0 .0 1和 P≤ 0 .0 5 ） ;心率变异性时域指标 SDNN,SDANN ,SDNNindex及 r MSSD均较对照组明显降低 （P<0 .0 1） ;血浆多巴胺水平与 HRV呈明显的负相关 （SDNN:r=- 0 .45 ,P≤ 0 .0 1;SDANN:r=- 0 .43,P≤ 0 .0 1;SDNN index:r=- 0 .5 1,P<0 .0 1;r MSSD:r=- 0 .49,P<0 .0 1） ,血浆去甲肾上腺素水平亦呈类似的相关性。结论 :尿毒症心脏交感神经病变以血浆儿茶酚胺水平升高和心率变异性降低为特点 ,提示交感神经作用代偿性增强     

本刊严正声明

<正>根据有关读者举报并经本刊初步查证,近一段时间来有人冒充本刊名义和盗用本刊的合法刊号(ISSN1674-3806/CN45-1365/R)进行非法出版活动(该非法出版物的编辑部地址为:北京市100036信箱27分箱;邮政编号:100036;联系电话:010-87013678;网址:http://www.zglcxyx010.com;E-mail:zglcxyx010@126.com、ZGLCXYX@163.com),严重地侵犯本刊的合法权益,损害了本刊的名义,在社会上造成     

本刊严正声明

根据有关读者举报并经本刊初步查证,近一段时间来有人冒充本刊名义和盗用本刊的合法刊号(ISSN1674-3806/CN45-1365/R)进行非法出版活动(该非法出版物的编辑部地址为:北京市100036信箱27分箱;邮政编号:100036;联系电话:010-87013678;网址:http://www.zglcxyx010.com;E-mail:zglcxyx010@126.com、ZGLCXYX@163.com),严重地侵犯本     

中华心血管病杂志2004年度继续教育园地思考多选题答案

第 1期心血管超声医学的研究进展: 1.D; 2.C; 3.B; 4 A。β-受体阻滞剂在心肌梗死中的应用: 1.D; 2.D; 3.A、B、C。第 2期贯彻循证医学的原则作好动脉粥样硬化的预: 1.D; 2.B、D; 3.C; 4.B、C。高血压病肾脏损害的诊断与防治: 1.A、B、C、D; 2.A、B、D; 3.D。第 3期易损斑块及易损患者的新定义及危险分层: 1.A、B、C、D; 2.A、B、C; 3.D; 4.A、B、C、D。β受体阻滞剂治疗慢性心力衰竭临床试验的启示: 1 C;2 B; 3 D; 4 A。第 4期冠心病整体防治中他汀类药物的重要地位: 1.A、B、C、D; 2.A; 3.C。高血压与心力衰…     

Levels of plasma des-γ-carboxy protein C and prothrombin in patients with liver diseases

AIM: To study the plasma des-γ-carboxy protein C activity, antigen and prothrombin levels in patients with liver diseases and their clinical significance. METHODS: Plasma protein C activity (PC:C) was detected by chromogenic assay and antigen (PC:Ag) and des-γ-carboxy protein C (DCPC) were detected by ELISA. Total prothrombin and unabsorbed prothrombin in plasma were detected by ecarin chromogenic assay. RESULTS: Compared with the control, the levels of PC:C and PC:Ag in patients with hepatocellular carcinoma (HCC) and liver cirrhosis (LC) were lower (PCC: 104.65&#177;23.0%,62.50&#177;24.89%, 56.75&#177;20.14%, PC:Ag: 5.31&#177;1.63 μg/mL, 2.28&#177;1.15 μg/mL, 2.43&#177;0.79 μg/mL, P&lt;0.05). The levels of PC:Ag in patients with acute viral hepatitis (AVH) also was lower (2.98&#177;0.91 μg/mL, P&lt;0.01), but PC:C was close to the control (93.76&#177;30.49%, P&gt;0.05). The levels of DCPC in patients with HCC were remarkably higher (0.69&#177;0.29 μg/mL,1.18&#177;0.63 μg/mL, 0.45&#177;0.21 μg/mL, P&lt;0.05) and its averagewas up to 50% of total PC:Ag. But those of DCPC in patients with AVH were not significantly different from the control. The levels of total prothrombin were lower in patients with LC, but higher in patients with HCC. The levels of unabsorbed prothrombin were predominantly higher than those of other groups. CONCLUSION: PC:C and PC:Ag in patients with liverdiseases (except PC:C in AVH) were lower. The total prothrombin was lower in patients with LC. The higher level of unabsorbed prothrombin may be used as a scanning marker for HCC. DCPC may be used as a complementary marker in the diagnosis of HCC.     

书籍及统计学符号的著录方法

<正>[书籍]著者(列名格式与期刊作者相同).书名.卷次:版次(如是第1版可不标出).出版者.年份:引文页码(如专著整体做为文献引用可不著录页码.或:析出文献作者.析出文献题名//专著责任者.书名.卷次:版次(如是第1版可不标出).出版地:出版者.年份:析出     

幽门螺杆菌HPAG1_1448基因生物信息学分析

目的 对幽门螺杆菌(HP)HPAG1_1148基因进行生物信息学分析,为HP疫苗和细菌学研究奠定基础.方法 应用Omiga 2.0等生物软件和Swiss-port、GenBank等数据库分析HPAG1_1148基因的变异性及其表达蛋白的化学性质、分子结构、保守区、抗原结构域、抗原表位.结果 该基因同源性为93.7%～97.4%,表达蛋白N端有长约23 aa的信号肽,为分泌型蛋白,具有抗原结构域、B细胞抗原表位及与胞膜孔道蛋白相似的保守区.结论 该基因具有较高保守性,表达蛋白是一种分泌型外膜蛋白,可能具有转运功能和抗原分子结构特征,有望成为HP疫苗.     

Cloning and sequence analysis of gene oipA encoding an outer membrane protein of human Helicobacter pylori

AIM: To construct a recombinant E. coli strain that would highly express the proinflammatory outer membrane protein of human Helicobacter pylori (H pylori). METHODS: The oipA DNA was amplified by PCR, inserted into pET-32a, and transformed into Top10 E. coli strain. This recombinant plasmid of Top10 was sent out for nucleotide sequence analysis. Finally this sequence AF479754 was compared with HP0638 and JHP0581. RESULTS: The sequence of the aim gene was obtained. It had 924 base pairs. The identity was 95.32% against HP0638, 95.02% against JHP0581, which was higher than the identity between HP0638 and JHP0581. CONCLUSION: Although the aim gene was obtained, but it was different from the published sequence of GenBank. It is not clear what makes this difference. Maybe it is because different strain was used or because there were some variations. So more researches are required to prove it.     

幽门螺杆菌热休克蛋白A亚单位编码基因的克隆与序列分析

目的 幽门螺杆菌(Helicobacter pylori)热休克蛋白A亚单位的编码基因(hspA)克隆和序列分析，为H.pyiori基因工程疫苗的研究奠定基础。方法 应用PCR方法获得国内分离H.pylori菌株MEI，HP27和国际标准参考株H.pylori的hspA基因，通过定向克隆的方法分别插入克隆载体pNEB193中，用质粒酶切电泳和特异PCR方法鉴定重组质粒。克隆基因经测序后进行核苷酸和氨基酸的同源性比较。结果 重组质粒经双酶切后得到0．35kb的hspA基因片段，特异PCR可扩增出hspA基因片段，证实H.pylori hspA基因的重组克隆质粒构建成功。经测序，国内分离HpMEL-HP27的hspA基因全长357bp(Genbank收录号：AY295084)，编码由118个氨基酸残基组成的肽链，hspA基因序列与GenBank公布的H.pylorl相应基因同源性高达95．20％～97．48％，氨基酸序列同源性在95．76％～97．46％之间。结论 克隆了H．pylori菌株MEL-HP27的hspA基因，其核酸序列与国际参考株NCTC11637同源性为97．48％。     

中国幽门螺杆菌强细胞毒株和弱细胞毒株vacA基因全长序列特征与VacA活性的关系

目的:探讨中国幽门螺杆菌(Helicobacter pylori,H.pylori)强细胞毒株和弱细胞毒株vacA基因序列差异对其VacA活性的影响.方法:从GenBank数据库下载4个强细胞毒株和4个弱细胞毒株vacA基因全长DNA和氨基酸序列,利用DNAMAN、lasergene 7.0、MEGA 5.03个生物信息学软件对其进行分析.结果:(1)中国H.pylori强细胞毒株、弱细胞毒株和西方强细胞毒株60190株3者之间在vacA基因序列上都存在明显的差异,这些差异主要集中在vacA基因p55结构域,表现为疏水性氨基酸与极性氨基酸之间的转换;(2)弱细胞毒株还存在多个插入变异位点;(3)强细胞毒株和弱细胞毒株,中国和西方分离株在系统发育树中分别聚类为不同的谱系.结论:vacA基因序列差异和插入突变可能是导致中国不同H.pylori VacA活性差异的重要原因.     

幽门螺杆菌oipA基因的开关状态及其与胃疾病的关系

目的:了解幽门螺杆菌(Hp)oipA基因的开关状态并探讨其与胃疾病的关系.方法:收集活检胃组织标本360份,对组织快速尿素酶实验阳性和部分阴性的活检标本进行Hp分离培养,分离菌经革兰染色镜检、尿素酶实验和16S rRNA PCR鉴定后,PCR扩增其oipA基因,测序分析oipA信号区的开关状态,分析功能性oipA与临床疾病之间的关联性.结果:组织标本分离培养获得106株Hp,其中72株cagA基因阳性,87株oipA信号区基因阳性.经测序分析80株oipA基因信号区呈开放状态,为功能性oipA.87株Hp中有1株oipA基因信号区呈关闭状态,6株开关状态不能确定.在胃溃疡和萎缩性胃炎中,功能性oipA的检出率[分别为12/12和78.26%(18/23)]均显著高于cagA[分别为58.33%switch status和39.13%switch status].结论:功能性oipA与萎缩性胃炎和胃溃疡关系密切.     

幽门螺杆菌尿素酶B亚单位编码基因的克隆与序列分析

目的 幽门螺杆菌(Helicobacter pylori)尿素酶B亚单位的编码基因(ureB)的克隆和序列分析，为H．pylori基因工程疫苗的研究奠定基础。方法应用PCR方法获得国内分离H．pylori菌株MEI，HP27和国际标准参考株H．pylori的ureB基因，通过定向克隆的方法分别插入克隆载体pNEB193中，用质粒酶切电泳和特异PCR方法鉴定重组质粒。克隆基因经测序后进行核苷酸和氨基酸的同源性比较。结果 重组质粒经双酶切后得到1．71kb的ureB基因片段，特异PCR可扩增出ureB基因片段，证实H．pylori ureB基因的重组克隆质粒构建成功。经测序，国内分离H．pylori MEL-HP27的ureB基因全长1710bp( Genbank收录号：AY295085)，编码由569个氨基酸残基组成的肽链，ureB基因序列与GenBank公布的H．pylori相应基因同源性高达96．08％～98．30％，氨基酸序列同源性在98．77％．99．82％之间。结论 成功克隆了MEL-HP27菌株的ureB基因，其核苷酸序列与国际参考株NCTC11637的同源性为97．67％。     

Importance of Helicobacter pylori oipA in clinical presentation,gastric inflammation,and mucosal interleukin 8 production

BACKGROUND & AIMS: Disease-associated virulence factors of Helicobacter pylori may not be independent of one another. The aim was to determine which H. pylori virulence factor(s) was the most important predictor of severity of gastric inflammation or clinical outcome. METHODS: cag Pathogenicity island (PAI), vacA babA2, and iceA status were determined by polymerase chain reaction (PCR). oipA functionality was based on switch status determined by PCR-based sequencing. A backward stepwise multiple regression analysis was performed to determine which factor(s) was the most discriminating for clinical outcome as well as the relationship to mucosal histology (H. pylori density, neutrophil infiltration, intestinal metaplasia, and gastric atrophy) and mucosal interleukin 8 (IL-8) production. RESULTS: H. pylori were obtained from 247 patients (86 with gastritis, 86 with duodenal ulcer, and 75 with gastric carcinoma). Although oipA status was closely linked to specific cag PAI, vacA, and babA2 genotypes, only oipA status remained in the final model to discriminate duodenal ulcer from gastritis (adjusted odds ratio [OR] = 5 and 95% confidence interval [CI] = 2.1-11.9). Among the factors, only a functional oipA was significantly associated with high H. pylori density, severe neutrophil infiltration, and high mucosal IL-8 levels (P < 0.001). oipA status had no relationship to gastric atrophic changes. CONCLUSIONS: oipA functional status was related to clinical presentation, H. pylori density, and gastric inflammation. cag PAI, babA2, or vacA status appear important only as surrogate markers for a functional oipA gene.     

Helicobacter pylori infection in mice: Role of outer membrane proteins in colonization and inflammation

BACKGROUND & AIMS: Mouse models of Helicobacter pylori infection have yielded variable results with respect to colonization and inflammation. We examined whether outer membrane proteins (OMPs) or the cag pathogenicity island (PAI) was responsible for some of this variability. METHODS: C57BL/6 mice received clinical H. pylori isolates with different genotypes for the cag PAI and OMP gene switch status, as well as isogenic gene knockout mutants for cagE, oipA, babA2, hopZ, cagE/oipA, or oipA/hopZ. Bacterial density, histology, and mucosal cytokine/chemokine levels were measured after 4 and 12 weeks. RESULTS: oipA, hopZ, hopO, and hopP switch status influenced both H. pylori density and colonization ability in mice. When 2 or more of the genes were "off," colonization rates were markedly reduced compared with those for strains with all genes "on" (from 58% to 0% after 12 weeks). oipA knockout mutants caused reduced inflammation and decreased mucosal interleukin 6 messenger RNA and KC messenger RNA and protein levels. H. pylori density and colonization ability were reduced if hopO or hopP switch status was changed from "on" to "off." There was a close relationship (r > 0.7) between the H. pylori density of the gastric mucosa of humans and mice when using the same H. pylori strains. CONCLUSIONS: Many of the differences reported with mouse models may reflect subtle unrecognized differences (e.g., switch status of an OMP gene), emphasizing the necessity of characterizing isolates before and after experiments. The mouse model may be suitable for investigating factors related to colonization ability, H. pylori density, and gastric mucosal inflammation.     

Prediction of promiscuous T-cell epitopes in the Zika virus polyprotein:An in silico approach

Objective:To predict immunogenic promiscuous T-cell epitopes from the polyprotein of the Zika virus using a range of bioinformatics tools.To date,no epitope data are available for the Zika virus in the IEDB database.Methods:We retrieved nearly 54 full length polyprotein sequences of the Zika virus from the NCBI database belonging to different outbreaks.A consensus sequence was then used to predict the promiscuous T cell epitopes that bind MHC 1 and MHC II alleles using Propred1 and Propred immunoinformatic algorithms respectively.The antigencity predicted score was also calculated for each predicted epitope using the Vaxi Jen 2.0 tool.Results:By using Pro Pred1,23 antigenic epitopes for HLA class I and 48 antigenic epitopes for HLA class II were predicted from the consensus polyprotein sequence of Zika virus.The greatest number of MHC class I binding epitopes were projected within the NS5(21%),followed by Envelope(17%).For MHC class II,greatest number of predicted epitopes were in NS5(19%) followed by the Envelope,NS1 and NS2(17% each).A variety of epitopes with good binding affinity,promiscuity and antigenicity were predicted for both the HLA classes.Conclusion:The predicted conserved promiscuous T-cell epitopes examined in this study were reported for the first time and will contribute to the imminent design of Zika virus vaccine candidates,which will be able to induce a broad range of immune responses in a heterogeneous HLA population.However,our results can be verified and employed in future efficacious vaccine formulations only after successful experimental studies.     

Cloning and sequencing of cagA gene fragment of Helicobacter pylori with coccoid form

AIM: To clone and sequence the cagA gene fragment of Helicobacter pylori (H pylori) with coccoid form. METHODS: H pylori strain NCTC11637 were transformed to coccoid form by exposure to antibiotics in subinhibitory concentrations. The coccoid H pylori was collected. cagA gene of the coccoid H pylori strain was amplified by PCR. After purified, the target fragment was cloned into plasmid pMD-18T. The recombinant plasmid pMD-18T-cagA was transformed into E.coli JM109. Positive clones were screened and identified by PCR and digestion with restriction endonucleases. The sequence of inserted fragment was then analysed. RESULTS: cagA gene of 3,444 bp was obtained from the coccoid H pylori genome DNA. The recombinant plasmid pMD-18T-cagA was constructed, then it was digested by BamH I+Sac I, and the product of digestion was identical with the predicted one. Sequence analysis showed that the homology of coccoid and the reported original sequence H pylori was 99.7%. CONCLUSION: The recombinant plasmid containing cagA gene from coccoid H pylori has been constructed successfully. The coccoid H pylori contain completed cagA gene, which may be related to pathogenicity of them.