高效液相法测定更昔洛韦的含量

目的:应用高效液相法测定更昔洛韦中的有关物质。方法:用PhenomenexLuna C18色谱柱,以0.02mol/L醋酸铵缓冲液(pH4.5)-甲醇(95:5)为流动相,流速为1ml/min,检测波长为254nm。结果:最低检出限鸟嘌呤为20ng,阿昔洛韦为12.5ng,其他有关物质等。从而更昔洛韦计为20ng。试验结果显示,更昔洛韦与阿昔洛韦、鸟嘌呤及其降解产物的分离度均能达到要求。结论:应用高效液相法灵敏度高,重现性好,结果准确,专属性强,可用已知杂质法测定更昔洛韦中的鸟嘌呤、阿昔洛韦,用自身对照法测定其他有关物质。     

HPLC测定注射用更昔洛韦的含量

注射用更昔洛韦是更昔洛韦加适量氢氧化钠溶液经冷冻干燥制成的无菌制品。卫生部药品标准[ws-349(X-273)-98]收载的注射用更昔洛韦含量测定方法是采用紫外分光光度法。因更昔洛韦的结构与阿昔洛韦相似,市场上已出现用阿昔洛韦冒充更昔洛韦的情况。紫外分光光度法专属性不强,不能准确辨别真伪。本实验室用HPLC法测定注射用更昔洛韦含量,具有快速准确、灵敏度高、专属性强的特点,可作为其可靠的检测方法。     

阿昔洛韦与更昔洛韦治疗小儿水痘的疗效对比

目的:分析比较阿昔洛韦与更昔洛韦治疗小儿水痘的疗效。方法:选取水痘患儿100例作为本次研究的对象,分为阿昔洛韦组(A组)与更昔洛韦组(B组),两组患儿在基础性治疗同时,分别注射阿昔洛韦和更昔洛韦,比较两组患儿疗效治疗。结果:两组患儿的总有效率、开始结痂日、完全结痂日、疼痛减轻日和疼痛消失日,B组患儿均明显优于A组(P<0.01)。结论:更昔洛韦治疗小儿水痘的临床效果明显优于阿昔洛韦治疗。     

HPLC测定注射用更昔洛韦的含量

注射用更昔洛韦是更昔洛韦加适量氢氧化钠溶液经冷冻干燥制成的无菌制品。卫生部药品标准[ws-349（X-273）-98]收载的注射用更昔洛韦含量测定方法是采用紫外分光光度法。因更昔洛韦的结构与阿昔洛韦相似，市场上已出现用阿昔洛韦冒充更昔洛韦的情况。紫外分光光度法专属性不强，不能准确辨别真伪。本实验室用HPLC法测定注射用更昔洛韦含量。具有快速准确、灵敏度高、专属性强的特点，可作为其可靠的检测方法。[第一段]     

更昔洛韦治疗成人病毒性脑炎的临床研究

目的 比较更昔洛韦与阿昔洛韦治疗成人病毒性脑炎的临床疗效.方法 66例患者随机分为两组,分别给予更昔洛韦和阿昔洛韦,比较其主要临床症状及体征恢复的时间.结果 更昔洛韦组取得显著临床疗效的时间比阿昔洛韦组缩短(P<0.05).结论 更昔洛韦治疗病毒性脑炎的疗效优于阿昔洛韦.     

更昔洛韦眼用凝胶和阿昔洛韦滴眼液治疗单疱病毒性角膜炎的效果分析

目的:比较更昔洛韦眼用凝胶和阿昔洛韦滴眼液治疗单疱病毒性角膜炎的疗效。方法:选取90例单疱病毒性角膜炎患者作为观察对象,根据入院时间先后将其分为观察组和对照组各45例。观察组给予更昔洛韦眼用凝胶治疗,对照组给予阿昔洛韦滴眼液治疗,比较两组疗效,统计两组不良反应发生率和6个月内的复发率。结果:观察组治疗有效率为93.33%(42/45),明显大于对照组的77.78%(35/45),差异具有统计学意义(P<0.05);观察组不良反应发生率和复发率均明显低于对照组,差异具有统计学意义(P<0.05)。结论:更昔洛韦眼用凝胶治疗单疱病毒性角膜炎效果优于阿昔洛韦滴眼液效果。     

更昔洛韦滴眼液治疗流行性急性角结膜炎的临床对比研究

目的:研究更昔洛韦滴眼液对流行性急性角结膜炎的临床疗效。方法:采用随机双盲的方法,将流行性急性角结膜炎患者随机分为2组,分别使用更昔洛韦滴眼液与阿昔洛韦滴眼液进行治疗,8次/日,治疗8天,观察两组患者的治疗效果。结果:82例完成本研究,更昔洛韦滴眼液治疗组总有效率明显高于B组,差异有显著性(P<0.05)。结论:更昔洛韦滴眼液是一种安全有效的治疗流行性急性角结膜炎的药物,同阿昔洛韦滴眼液相比,更昔洛韦滴眼液治疗流行性急性角结膜炎更有效。     

单用更昔洛韦与联合阿昔洛韦对小儿CMV肺炎疗效的比较

目的:比较单用更昔洛韦与联合阿昔洛韦治疗小儿巨细胞病毒肺炎的效果。方法:将确诊为巨细胞病毒肺炎的158例患儿随机分成A、B两组:A组(78例)更昔洛韦5 mg/(kg.次),每12小时静脉滴注1次,治疗14 d后改为1次/d,维持7 d;B组80例,在A组基础上加用阿昔洛韦10 mg/(kg.次),每8小时静脉滴注1次,持续14 d。结果:A组有效率为83.3%,不良反应率为16.7%;B组有效率为93.8%、不良反应率为20.0%,两组之间有效率比较,差异有统计学意义(P<0.05),不良反应率比较,差异无统计学意义(P>0.05)。结论:更昔洛韦联合阿昔洛韦治疗小儿巨细胞病毒肺炎的效果更好,且不良反应无比较差异无统计学意义(P>0.05)。     

更昔洛韦治疗成人水痘30例报告

目的了解更昔洛韦治疗成人水痘的临床效果。方法将30例成人水痘患者随机分成两组,每组15例。两组均同时外用5%硫磺炉甘石洗剂,观察组给予更昔洛韦250mg/d静脉滴注,共5天,对照组给予阿昔洛韦200mg口服,每天5次,共5天。结果观察组发热、止疱时间比对照组显著缩短(P均<0.01)。结论更昔洛韦治疗成人水痘效果明显优于阿昔洛韦。     

更昔洛韦联合醒脑静治疗病毒性脑炎

目的:探讨更昔洛韦联合醒脑静治疗病毒性脑炎的临床疗效。方法:将62例病毒性脑炎患者随机分成治疗组和对照组,治疗组予综合治疗外,加更昔洛韦与醒脑静,对照组予综合治疗加阿昔洛韦。结果:治疗组在退热、抽搐控制、头痛呕吐消失、意识转清等方面的平均时间较对照组明显缩短,差异有统计学意义(P<0.01)。结论:更昔洛韦联合醒脑静治疗病毒性脑炎较单独用更昔洛韦疗效更好。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.